Claims
- 1. A method for quantifying cholesterol in high density lipoprotein, comprising a first step of erasing cholesterol in lipoproteins other than high density lipoprotein in a test sample by contacting the test sample with cholesterol esterase and cholesterol oxidase in the absence of a surfactant which acts on high density lipoprotein in a buffer with a pH of 5 to 8 in the presence of a divalent metal cation, and removing generated hydrogen peroxide, and a second step of adding a surfactant having an HLB of 13 to 14, which surfactant specifically acts on high density lipoprotein product of said first step and enzymatically quantifying cholesterol in high density lipoprotein by quantifying hydrogen peroxide generated by actions of said cholesterol esterase and said cholesterol oxidase.
- 2. The method according to claim 1, wherein said surfactant which specifically acts on high density lipoprotein is a polyalkylene oxide derivative.
Priority Claims (1)
Number |
Date |
Country |
Kind |
8-344649 |
Dec 1996 |
JP |
|
Parent Case Info
This application is the national phase under 35 U.S.C. §371 of prior PCT International Application No. PCT/JP97/04442 which has an International filing date of Dec. 4, 1997 which designated the United States of America, the entire contents of which are hereby incorporated by reference.
PCT Information
Filing Document |
Filing Date |
Country |
Kind |
PCT/JP97/04442 |
|
WO |
00 |
Publishing Document |
Publishing Date |
Country |
Kind |
WO98/26090 |
6/18/1998 |
WO |
A |
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A |
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