Claims
- 1. A method of immunologically determining human acid glutathione S-transferase in a human assay sample, which comprises bringing the assay sample into contact with a first antibody bound to an insoluble solid carrier and a labelled second antibody, either the first or second antibody being a polyclonal antibody capable of binding to human acid glutathione S-transferase or an Fab, Fab' or F(ab').sub.2 fragment of the polyclonal antibody, and the other antibody being a monoclonal antibody capable of specifically binding to human acid glutathione S-transferase or an Fab, Fab' or F(ab,).sub.2 fragment of the monoclonal antibody, wherein said monoclonal antibody is a monoclonal antibody 6A produced by hybridoma FERM BP-3023.
- 2. A method of diagnosing cancer in a human digestive organ, which comprises
- (i) using a first antibody bound to an insoluble solid carrier and a labelled second antibody, either the first or second antibody being a polyclonal antibody capable of binding to human acid glutathione S-transferase or an Fab, Fab' or F(ab').sub.2 fragment of the polyclonal antibody, and the other antibody being a monoclonal antibody capable of specifically binding to human acid glutathione S-transferase or an Fab, Fab' or F(ab').sub.2 fragment of the monoclonal antibody,
- (ii) bringing a human assay sample into contact with the first and second antibodies,
- (iii) determining the amount of human acid glutathione S-transferase contained in the sample, and
- (iv) diagnosing the onset of cancer in a human digestive organ, the state of its progress or its regression on the basis of the determined amount of human acid glutathione S-transferase,
- wherein said monoclonal antibody is a monoclonal antibody 6A produced by hybridoma FERM BP-3023.
- 3. The method of claim 1 or 2 in which the first antibody is a polyclonal antibody capable of binding to human placenta-derived glutathione S-transferase or an Fab, Fab' or F(ab').sub.2 fragment thereof, and the second antibody is a monoclonal antibody capable of specifically binding to human placenta-derived glutathione S-transferase.
- 4. The method of claim 1 or 2 in which the first antibody is a monoclonal antibody capable of specifically binding to human placenta-derived glutathione S-transferase or an Fab, Fab' or F(ab').sub.2 fragment thereof, and the second antibody is a polyclonal antibody capable of binding to human placenta-derived glutathione S-transferase.
- 5. The method of any one of claims 1 or 2 in which the polyclonal antibody binds to a region of the amino acid sequence of a human placenta-derived glutathione S-transferase molecule which ranges from the 92nd amino acid residue Val to the 209th (C-terminus) amino acid residue Gln from the N-terminus.
- 6. The method of claim 3 in which the polyclonal antibody binds to a region of the amino acid sequence of a human placenta-derived glutathione S-transferase molecule which ranges from the 92nd amino acid residue Val to the 209th (C-terminus) amino acid residue Gln from the N-terminus.
- 7. The method of claim 4 in which the polyclonal antibody binds to a region of the amino acid sequence of a human placenta-derived glutathione S-transferase molecule which ranges from the 92nd amino acid residue Val to the 209th (C-terminus) amino acid residue Gln from the N-terminus.
- 8. The method of any one of claims 1 or 2 in which the human assay sample is a human serum or plasma sample.
- 9. The method of claim 3 in which the human assay sample is a human serum or plasma sample.
- 10. The method of claim 4 in which the human assay sample is a human serum or plasma sample.
- 11. The method of any one of claims 1 or 2 in which the immune reaction is carried out in the presence of a protein having an average molecular weight of 16,000 to 50,000 and an isoelectric point of 1.0 to 5.0 or a mixture containing the protein as an antigen-antibody reaction regulating agent, the final concentration of the antigen-antibody reaction regulating agent being adjusted to 0.02 to 0.9% by weight.
- 12. The method of claim 3 in which the immune reaction is carried out in the presence of a protein having an average molecular weight of 16,000 to 50,000 and an isoelectric point of 1.0 to 5.0 or a mixture containing the protein as an antigen-antibody reaction regulating agent, the final concentration of the antigen-antibody reaction regulating agent being adjusted to 0.02 to 0.9% by weight.
- 13. The method of claim 4 in which the immune reaction is carried out in the presence of a protein having an average molecular weight of 16,000 to 50,000 and an isoelectric point of 1.0 to 5.0 or a mixture containing the protein as an antigen-antibody reaction regulating agent, the final concentration of the antigen-antibody reaction regulating agent being adjusted to 0.02 to 0.9% by weight.
- 14. The method of claim 11 in which the antigen-antibody reaction regulating agent is skimmed milk.
- 15. The method of claim 12 in which the antigen-antibody reaction regulating agent is skimmed milk.
- 16. The method of claim 13 in which the antigen-antibody reaction regulating agent is skimmed milk.
- 17. A reagent system for immunological determination of human acid glutathione S-transferase in a human assay sample, comprising a first antibody bound to an insoluble solid carrier and a labelled second antibody, either the first or second antibody being a polyclonal antibody capable of binding to human placenta-derived gluthathione S-transferase or an Fab, Fab' or F(ab').sub.2 fragment of the polyclonal antibody, and the other antibody being a monoclonal antibody capable of specifically binding to human placenta-derived glutathione S-transferase or an Fab, Fab' or F(ab').sub.2 fragment of the monoclonal antibody,
- wherein said monoclonal antibody is a monoclonal antibody 6A produced by hybridoma FERM BP-3023.
- 18. A kit for immunological determination of human acid glutathione S-transferase in a human assay sample, comprising (a) a first antibody bound to an insoluble solid carrier, (b) a labelled second antibody, either the first or second antibody being a polyclonal antibody capable of binding to human placenta-derived glutathione S-transferase or an Fab, Fab' or F(ab').sub.2 fragment of the polyclonal antibody, and the other antibody being a monoclonal antibody capable of specifically binding to human placenta-derived glutathione S-transferase or an Fab, Fab' or F(ab').sub.2 fragment of the monoclonal antibody, (c) a dissolving agent, (d) a washing agent, and when an enzyme-labelled antibody is used, (e) a substrate for measuring the enzyme activity and a reaction stopper therefor,
- wherein said monoclonal antibody is a monoclonal antibody 6A produced by the hybridoma FERM BP-3023.
- 19. The system of claim 17 or the kit of claim 18 in which the first antibody is a polyclonal antibody capable of binding to human acid glutathione S-transferase or an Fab, Fab' or F(ab').sub.2 fragment thereof, and the second antibody is a monoclonal antibody capable of specifically binding to human acid glutathione S-transferase.
- 20. The system of claim 17 or the kit of claim 18 in which the first antibody is a monoclonal antibody capable of specifically binding to human acid glutathione S-transferase or an Fab, Fab' or F(ab').sub.2 thereof, and the second antibody is a polyclonal antibody capable of binding to human acid glutathione S-transferase or an Fab, Fab' or F(ab').sub.2 fragment thereof.
- 21. The system of claim 17 or the kit of claim 18 in which the polyclonal antibody binds to a region of the amino acid sequence of a glutathione S-transferase molecule derived from human placenta which ranges from the 92nd amino acid residue Val to the 209th (C-terminus) amino acid residue Gln from the N-terminus.
Priority Claims (3)
Number |
Date |
Country |
Kind |
63-19602 |
Feb 1988 |
JPX |
|
63-268292 |
Oct 1988 |
JPX |
|
63-282546 |
Nov 1988 |
JPX |
|
Parent Case Info
This application is a continuation of now abandoned application, Ser. No. 07/731,367, filed Jul. 16, 1991, which is a continuation-in-part of now abandoned application, Ser. No. 07/304,648 filed Feb. 1, 1989.
US Referenced Citations (2)
Number |
Name |
Date |
Kind |
4486530 |
David et al. |
Dec 1984 |
|
5298393 |
Urushizaki et al. |
Mar 1994 |
|
Foreign Referenced Citations (1)
Number |
Date |
Country |
0245520 |
Jun 1987 |
EPX |
Non-Patent Literature Citations (2)
Entry |
I. Y. Wang et al., "Multiple Ya Subunits of Glutathione S-Transferase Detected by Monoclonal Antibodies", Articles of Bichemistry and Biophysics, vol. 245, No. 2, Mar. 1986, pp. 543-547. |
Shiratori, et al., Cancer Res., 1987, 47(24, Pt. 1), 6806-9, "Immunohistochemical Detection of the Placental Form of Glutathione S-transferase in Dysplastic and Neoplastic Human Uterine Cervix Lesions". |
Continuations (1)
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Number |
Date |
Country |
Parent |
731367 |
Jul 1991 |
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Continuation in Parts (1)
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Number |
Date |
Country |
Parent |
304648 |
Feb 1989 |
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