Claims
- 1. A method for diagnosing Alzheimer's Disease in an individual, the method comprising detecting carboxyl-terminal truncated apoE in a biological sample from the individual.
- 2. The method of claim 1, wherein the biological sample is blood.
- 3. The method of claim 1, wherein the biological sample is serum.
- 4. The method of claim 1, wherein the carboxyl-terminal truncated apoE has a molecular weight of about 14-20 kDa.
- 5. The method of claim 1, wherein the carboxyl-terminal truncated apoE comprises amino acids 244-260 of apoE.
- 6. The method of claim 1, wherein apoE is apoE4.
- 7. The method of claim 1, wherein apoE is apoE3.
- 8. The method of claim 1, wherein apoE is a mixture of apoE3and apoE4.
- 9. The method of claim 1, wherein said detecting step is detecting a level of carboxyl-terminal truncated apoE in the bodily fluid.
- 10. The method of claim 9, further comprising detecting a level of full length apoE in the biological sample from the individual; wherein a ratio of the level of carboxyl-terminal truncated apoE compared to the level of full length apoE in the biological sample that is greater than a ratio associated with a control biological sample from an individual not having Alzheimer's Disease is indicative of a diagnosis of Alzheimer's Disease.
- 11. The method of claim 10, wherein the carboxyl-terminal truncated apoE apoE has a molecular weight of about 14-20 kDa.
- 12. The method of claim 10, wherein the ratio is greater than about 1.5.
- 13. The method of claim 10, wherein the ratio is greater than about 2.
- 14. The method of claim 10, wherein the ratio is greater than about 3.
- 15. A kit for diagnosing Alzheimer's Disease, the kit comprising an antibody that binds to carboxyl-truncated apoE and instructions for using the antibody for diagnosing Alzheimer's Disease.
- 16. The kit of claim 15, further wherein the antibody is attached to a solid support.
- 17. The kit of claim 15, further comprising an antibody that specifically binds to a carboxyl-terminal portion of apoE.
- 18. The kit of claim 15, wherein the instructions for the diagnosis of Alzheimer's Disease direct the use of the kit to detect carboxyl-terminal truncated apoE in serum..
CROSS-REFERENCE
[0001] This application claims the benefit of U.S. Provisional Patent Application No. 60/400,016 filed Jul. 30, 2002, which application is incorporated herein by reference in its entirety.
Provisional Applications (1)
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Number |
Date |
Country |
|
60400016 |
Jul 2002 |
US |