Patent Application
20210330589
The invention relates to a method of encapsulating active ingredients in liposomes, comprising
Liposomes, which are also referred to as vesicles, are understood to mean membrane bubbles that may generally be in colloidally suspended form in a hydrophilic, especially aqueous, medium, and enclose a liquid phase, where the liquid phase is typically, albeit not necessarily, a hydrophilic, usually aqueous phase. The membrane shell that encloses the liquid phase is formed from a double layer or bilayer composed of two at least monomolecular layers, composed either of one and the same molecule or of different molecules, each of which have both a nonpolar, i.e. hydrophobic or lipophilic, component and a polar, i.e. hydrophilic or lipophobic, component and are referred to as amphiphilic on account of these properties. The amphiphilic compounds that are used here as membrane-forming molecules are usually lipids, for example phospholipids, sphingolipids, glycolipids, fatty acids or the like, although it is also possible to use other amphiphilic compounds as membrane components, for example lipopolysaccharides, tocopherols, squalenes, sterins or sterols, cholesterols etc. At the time of creation of the bilayer of liposomes, the amphiphilic compounds are arranged in accordance with their hydrophilic/hydrophobic properties, such that the hydrophobic components of the amphiphilic compounds are each directed toward one another, in order to have minimum contact with the phase to be encapsulated, for example hydrophilic or aqueous phase, while the hydrophilic components of the amphiphilic compounds are directed toward the hydrophilic or aqueous phase, for example, within and outside the liposome. On account of the thermodynamic drive to assume an energetically favorable form with a minimum surface area in such an alignment, liposomes typically have an essentially ball-shaped or spherical form. The amphiphilic compounds that form the bilayer(s) of the membrane of liposomes consequently hold together merely by virtue of noncovalent bonding forces, and for that reason the membrane has a primarily fluid character.
Over and above the customary scientific use of the term “liposome” or of the synonymous terms “vesicle”, liposomes in the context of the present disclosure also include colloid-chemical aggregates in the form of nanocapsules of any amphiphilic substances, polymer liposomes, lipid nanoparticles and mixtures of such aggregate formations with pure liposomes. Therefore, such colloid-chemical aggregates are always included in the context of the present disclosure, even though only the term “liposome” is used.
The size and exact shape of liposomes depend to a crucial degree on the chemical structure of the amphiphilic compound(s) that form the bilayer(s) of their membrane, and on the physicochemical properties of the typically hydrophilic or aqueous phase to be encapsulated, for example on its ionic strength, pH, osmolality and the like, and the respective production method. Liposomes may either have just a single bilayer, such liposomes being referred to as unilamellar (“unilamellar vesicles”, ULV), or they may have multiple bilayers in a respectively concentric arrangement, such liposomes being referred to as multilamellar (“multilamellar vesicles”, MLV). The average diameter here is generally between about 20 nm and about 100 μm, especially between about 25 nm and about 30 μm.
As already mentioned, the amphiphilic compounds that form the bilayer(s) of the membrane of liposomes, according to the desired pharmacodynamic profile of action, pharmacokinetic behavior, chemical and physical properties, for example size, size distribution, lamellarity, fluidity, permeability, zeta potential, phase transition temperature of the membrane etc., may be formed from the same or different molecules, for example from the group of lipids, where the bilayer may either have been formed from the same amphiphilic compound or from the same mixtures of multiple amphiphilic compounds, or the individual at least monomolecular layers of the bilayer may each have been formed from different amphiphilic compounds or from mixtures of different amphiphilic compounds. While reference is made in the former case to symmetric liposomes (the at least monomolecular layers of the bilayer of the membrane are of the same structure, but where the molecules of the layers are in opposite alignment with regard to their polar and nonpolar components), reference is made in the latter case to asymmetric liposomes (the at least monomolecular layers of the bilayer of the membrane are of different structure, where the molecules of the layers are again in opposite alignment with regard to their polar and nonpolar components).
While liposomes may also find use, for example, for studies of the biophysical properties of biomembranes, they are used primarily in the cosmetic sector and especially in the medical sector. It is especially possible here, by virtue of the liposomal formulation of active ingredients, such as medicaments and the like, to protect sensitive active ingredients from possible metabolization after application and to specifically lead them to the cells of the organism where the active ingredient is to display its effect, such that any side effects of the active ingredient in a liposomal formulation are reduced and efficacy can be increased, in order to be able to administer lower doses of the active ingredient. Furthermore, the encapsulation of active ingredients in liposomes can increase the plasma half-life. The active ingredient here is typically in the form of a usually hydrophilic, especially aqueous or else alcoholic for example, active ingredient solution in the liposome. Primarily hydrophilic active ingredients may be encapsulated more or less completely in the liposomes, whereas primarily lipophilic substances are more likely to be incorporated into the bilayer of the amphiphilic compound(s).
Accordingly, liposomes play a significant role in modern pharmacy, cosmetics and food technology as transport vehicle for active pharmaceutical or other ingredients, for improvement of skin moisture level or active ingredient absorption, or for high-grade food additives. Further fields of use mentioned merely by way of example for liposomes include drug delivery, synthetic chemistry in general, nanoscale reaction chambers and general technological developments in the fields of energy, optics, electronics, microfluidics, colloid chemistry, biosensors or related fields in which liposomes can be used.
Liposomes may additionally be coated with a polymer layer, for example based on polyethylene glycol (PEG), and/or at least the amphiphilic compound of the outer layer of the bilayer(s) that form(s) the membrane may have been modified with such a polymer, in which case reference is made to “PEGylated liposomes”. The polymer layer serves for steric protection of the membrane, and protects and reduces labeling (opsonization) and elimination by the immune system, as a result of which the liposomes can circulate for longer in the organism and be enriched, for example, in tumor tissue. While, as already mentioned above, the pharmacokinetics are influenced by the nature of the liposomes, especially by the amphiphilic compound(s) that form(s) the bilayer(s) of their membrane, and barely at all by the nature of the encapsulated active ingredient itself, drug targeting can be further optimized by PEGylation, which, for tumor therapy, for example, means maximum enrichment of the active ingredients in the tumor tissue. For that reason, many chemotherapeutics are currently administered as liposomal formulations (e.g. DaunoXome®, DepoCyt®, Doxil®/Caelyx® Marqibo®, Mepact®, Myocet®), in order to increase the therapeutic effects through the enrichment in the target tissue, but at the same time to reduce side effects as a result of reduced release in healthy tissue and organs.
Existing commercial production techniques for liposomes are based essentially on the following three alternative methods:
What is common to all the commercial production techniques for liposomes that have been developed to date is that, in the encapsulation, the typically hydrophilic or aqueous or possibly even alcoholic interior is identical to the exterior. Given a customary encapsulation capacity of about 1% to 15%, this means that 99% to 85% of the active ingredient has to be removed subsequently from the exterior and preferably reused. Subsequent remote loading of the liposomes by means of thermal methods or pH gradients is possible only for particular active ingredients and entails other disadvantages, for example reduced storage stability, additional processing steps and the like. Furthermore, a multitude of biogenic active pharmaceutical ingredients, especially proteins, are denatured by these methods.
A further particular shortcoming of the commercial production methods developed to date is the uniformity of the membrane, i.e. the inside and outside of the bilayer are more or less identical; the liposomes produced are consequently symmetric liposomes. In all naturally occurring biogenic membranes, however, the insides and outsides of the membrane are always different, which is true both with regard to their lipid composition and their protein composition. The asymmetry of biological membranes has an important physiological function for various cellular recognition and transport mechanisms. Accordingly, unlimited manipulability of the composition of the inside and outside of liposome membranes would be desirable, for example for the accumulation of active ingredients on the inside and of steric protective layers or receptors on the outside, which is currently possible to a limited degree only by subsequent processing steps.
It should be pointed out here that the term “monolayer” is typically understood to mean monomolecular layers of amphiphilic or interface-active compounds, for example of lipids, and an emulsion droplet consisting solely of a monomolecular or single layer of an amphiphilic compound or a group of amphiphilic compounds differs from liposomes in that the latter have at least one bilayer of two at least monomolecular layers of amphiphilic compounds, the polar and nonpolar regions of which have mutually opposite orientations in the monolayers that form the bilayer. In addition to this definition, in the context of the present disclosure, the term “monolayer” always also addresses all substances and substance classes capable of forming thin layers, for example polymers and proteins, which is especially also the case when these substance classes and substances, if appropriate, include or may include more than a single molecular layer. Analogously, “bilayer” in the context of the present disclosure is always also understood to mean the combination of two monolayers in the above sense. “Pre-liposomes” in the context of the present disclosure shall be understood to mean emulsion droplets having a monomolecular or single layer of an amphiphilic compound or of a group of amphiphilic compounds.
The option that underlies the invention of synthesizing monolayers from amphiphilic compounds to give a bilayer and dividing them off by means of centrifugal forces to form liposomes is fundamentally known from the prior art.
For instance, H. Träuble and E. Grell: “The formation of asymmetrical spherical lecithin vesicles”, Neuroscience Research Program Bulletin, 9, 373-3801 (1971), describes a method of encapsulating active ingredients in liposomes, wherein, in a step (a), an aqueous active ingredient solution is provided, which, in a step (b), is dispersed in a phase having zero or sparing miscibility therewith in the presence of one or more amphiphilic compounds in the form of lipids, such that at least one of the amphiphilic compounds accumulates as a monomolecular layer on the emulsified droplets of the active ingredient solution, i.e. forms pre-liposomes in the sense of the present disclosure. Moreover, in a step (c), a phase having zero or sparing miscibility with the continuous phase of the emulsion—an aqueous phase here—is provided, and the pre-liposomes thus produced are transferred from the emulsion to the aqueous phase by centrifugal forces. At the phase transition, the pre-liposomes are surrounded by a second monolayer of at least one of the amphiphilic compounds that has been enriched at the phase boundary, such that the liposome or vesicle is produced to obtain a bilayer. While the method is undoubtedly suitable for production of symmetric liposomes, the supposition is set out in the paper that it is also possible thereby to form liposomes having an asymmetric composition of the bilayer constituted from the various first amphiphilic compounds, but no evidence is supplied as to the implementability of this idea. This is because the unsolved problem in this concept is that the amphiphilic compounds that show the phase boundaries of a) the dispersed droplets and b) the phase boundary between the two immiscible phases cannot be separated from one another. On account of a lack of separation, the amphiphilic compounds can thus reach all interfaces by diffusion, which means that adequate asymmetry of the bilayer cannot be achieved. A further problem with this concept lies in the propensity of almost all amphiphilic compounds, for example phospholipids and the like, to form what are called organogels together with an organic phase and an aqueous phase (in this regard see also, for example, P. L. Luisi, R. Scartazzini, G. Haering, P. Schurtenberger: “Organogels from water-in-oil microemulsions”, Colloid Polym Sci, 268: 356-374 (1990)). These organogels become enriched at the phase boundary between the organic phase and the aqueous phase, and form a barrier layer which becomes increasingly more difficult to overcome and hinders the phase transfer.
A further development of the above-described method of Träuble and Grell is the method according to the paper by S. Pautot, B. J. Frisken, D. A. Weitz: “Engineering asymmetric vesicles”, Proceedings of the National Academy of Sciences USA, 100: 10718-10721 (2003a), which is based on the process designed by Träuble and Grell, but extends it with an organic interphase present during the centrifugation step between the emulsion containing the pre-liposomes that have already been provided with a monomolecular layer of at least one amphiphilic compound and the aqueous phase into which the pre-liposomes have been transferred from at least one amphiphilic compound with accumulation of the second monomolecular layer. Although this interphase is miscible with the organic water-in-oil emulsion with the pre-liposomes, it is separable in the centrifugal field on account of its difference in density and contains at least one amphiphilic compound of the outer layer of the bilayer, which is consequently more or less separated from the amphiphilic compound of the inner monolayer. In this way, the interphase gives rise to a sufficient diffusion boundary for the amphiphilic compounds at the interfaces, which makes it possible to form asymmetric bilayers. Here too, however, there is the problem of formation of organogels, especially at this phase boundary between the aqueous phase and the interphase. Moreover, particularly for the reason that the pre-liposomes provided with the (inner) monolayer of at least one amphiphilic compound must pass through the interphase before the (outer) monolayer of the further amphiphilic compound can accumulate, there is the risk of contamination of the liposomes with the organic interphase which is virtually impossible to remove subsequently. Finally, the process according to Pautot et al. is unsuitable for the formation of asymmetric bilayers in which further hydrophilic conjugates, for example polyethylene glycol (PEG), are bonded to the hydrophilic head groups on one side, because, in the method, the amphiphilic compounds that serve for incorporation into the outer monolayer first have to be dissolved in the organic phase, which limits the solubility of conjugates having large hydrophilic molecular components.
Moreover, aside from the unsolved problems described, none of the processes mentioned in the form presented is suitable for employment in a continuous process, in order thus to be able to use it on the industrial scale in an economically viable manner. In principle, the methods are all based on centrifuging a hydrophobic phase together with an aqueous phase. On conclusion of the production process for the liposomes, the two phases should be separated such that the aqueous phase can be utilized as product and the hydrophobic phase as auxiliary for the performance of the process.
It is therefore an object of the invention to provide, with at least very substantial avoidance of the aforementioned disadvantages, a simple and inexpensive method of encapsulating active ingredients in liposomes which permits the production of asymmetric liposomes with more or less freely adjustable different compositions of the monolayers of amphiphilic compounds that form the bilayer of their membrane, wherein the formation of organogels from the amphiphilic compounds that form the bilayer is minimized and continuous production of liposomes should advantageously also be enabled.
According to the invention, this object is achieved with a method of encapsulating active ingredients in liposomes of the type specified at the outset, which comprises the following steps:
The method according to the invention for production of liposomes is suitable both for production of symmetric liposomes (the first amphiphilic compound(s) is/are identical to the second amphiphilic compound(s)) and especially for production of asymmetric liposomes (the first amphiphilic compound(s) is/are different from the second amphiphilic compound(s)), wherein the first and second amphiphilic compound(s) can be chosen virtually freely in order to produce a monolayer of individually adjustable first or second amphiphilic compounds in each case.
In the prior art cited at the outset, it is thus the case, at first, in a manner known as such, in a step (a), that one or more active ingredient(s) to be encapsulated are dissolved in a solvent or in a solvent mixture in order to produce an active ingredient solution of the at least one active ingredient to be encapsulated. The at least one solvent may for example, albeit not necessarily, especially be a hydrophilic solvent, in which case possible options in practice are primarily physiologically benign water-based solvents including water, or isotonic solutions, and, for example, also alcohol-based solvents, especially based on ethanol.
In a step (b)—in this respect again analogously to the prior art outlined at the outset—this active ingredient solution is then emulsified in a first liquid having zero or sparing miscibility with the at least one solvent of the active ingredient solution or with a mixture of such first liquids in the presence of one or more first amphiphilic compound(s) to obtain a first emulsion, such that an at least monomolecular inner layer of the at least one first amphiphilic compound is accumulated on the droplets of the active ingredient solution emulsified in the first liquid. In this way, it is again the case that pre-liposomes are first generated in the context of the present disclosure, wherein the monomolecular layer of the at least one first amphiphilic compound forms the inner layer of the bilayer of the liposomes to be generated in the subsequent steps. Because the first emulsion, however, in a departure from the prior art, contains only the at least one first amphiphilic compound of the inner monolayer of the bilayer, the formation of pre-liposomes (also) containing the at least one second amphiphilic compound envisaged for the outer monolayer of the bilayer is reliably avoided, such that the greatest possible asymmetry of the bilayer can be achieved merely to that extent. The average droplet size and distribution of this first emulsion and consequently the average diameter of the pre-liposomes or of the liposomes to be generated therefrom may be adjusted in a customary manner, for example by corresponding input of mechanical forces, for example shear forces, in the generation of the first emulsion, and may be varied within broad intervals. During a period of a few minutes up to a few hours, the at least one first amphiphilic compound capable of pre-liposome or membrane formation is then able, as the (inner) monolayer, to form by diffusion at the phase boundary between the active ingredient solution droplets and the first liquid of the first emulsion. If the active ingredient solution is a customarily hydrophilic, for example aqueous and/or alcoholic, solution, the first liquid(s) is/are hydrophobic liquid(s) of zero or only very sparing miscibility therewith. The first amphiphilic substance may especially be lipids, for example phospholipids and the like, or any other amphiphilic compounds known for production of liposomes, or mixtures of such compounds, including those of the type specified at the outset.
As well as the provision of this first emulsion with the pre-liposomes composed of droplets of the active ingredient solution enclosed within the first layer of the at least one first amphiphilic compound, also provided in a step (c) is a mixture of zero or sparing miscibility with the first liquid of this first emulsion, i.e. with the continuous phase thereof, where this mixture is formed from a liquid phase of zero or sparing miscibility with the first liquid of the first emulsion in the above step (b) with the at least one second amphiphilic compound. In a departure from the prior art, consequently, what is provided is not just a “pure” liquid phase but rather a mixture of such a liquid phase with the at least one second amphiphilic compound which forms the at least monomolecular outer layer of the bilayer of the liposomes to be generated in the subsequent method steps, wherein the at least one second amphiphilic compound is consequently kept spatially separate from the at least one first amphiphilic compound which is present in the first emulsion and forms the at least monomolecular inner layer of the pre-liposomes emulsified therein. Because this mixture contains only the at least one second amphiphilic compound of the outer monolayer of the bilayer, the formation of pre-liposomes (also) containing the at least one first amphiphilic compound intended for the inner monolayer of the bilayer is avoided, such that, in this respect too, the greatest possible asymmetry of the bilayer can be achieved. The liquid phase of this mixture is especially a hydrophilic phase if the at least one first liquid of the first emulsion containing the pre-liposomes, i.e. the continuous phase thereof, is hydrophobic or the active ingredient solution is hydrophilic.
As soon as the (inner) monomolecular layer of the at least one first amphiphilic compound in the first emulsion generated in the above step (b) has attained a minimum density with which it will be capable of forming a bilayer at a later stage, in a subsequent step (d), the first emulsion with the droplets of the active ingredient solution emulsified therein having the at least monomolecular inner layer of the at least one first amphiphilic compound accumulated thereon, i.e. with the pre-liposomes generated in the above step (b), is contacted with the mixture of the liquid phase with the at least one second amphiphilic compound from the above step (c) to form a phase boundary between this first emulsion and this mixture, wherein at least the at least one second amphiphilic compound is enriched at this phase boundary. Any enrichment of an excess of the at least one first amphiphilic compound from the first emulsion at the phase boundary as well can be very substantially avoided here in a simple manner by adding the at least one second amphiphilic compound to the mixture in excess and/or adding the at least one first amphiphilic compound to the first emulsion in a proportion corresponding roughly to the proportion capable of being accumulated as the inner monolayer on the droplets of the active ingredient solution that are emulsified there.
A concluding step, step (e), finally comprises the centrifuging of the first emulsion from the above step (b) and the mixture from the above step (c) that is in contact therewith via the phase boundary in order to convert the droplets of the active ingredient solution that is present in the first emulsion and has the at least monomolecular inner layer of the at least one first amphiphilic compound accumulated thereon, i.e. the pre-liposomes prefabricated in the above manner in the context of the present disclosure, from the first liquid of the first emulsion into the liquid phase of the mixture, wherein, when it passes through the phase boundary, the at least one second amphiphilic compound enriched there is accumulated on the at least monomolecular inner layer of the at least one first amphiphilic compound of the droplets of the active ingredient solution to form an at least monomolecular outer layer thereof, in order to create the bilayer composed of the two at least monomolecular layers of the at least one first and second amphiphilic compounds. The pre-liposomes composed of the droplets of the active ingredient solution with the monomolecular (inner) layer of the at least one first amphiphilic compound accumulated thereon are consequently moved in the centrifugal force field, on account of their difference in density, from the first liquid, especially the hydrophobic liquid, of the first emulsion, i.e. from the continuous phase thereof, to the liquid phase, especially the hydrophilic phase, adjoining the phase boundary via the phase boundary. At this phase boundary, the at least one second amphiphilic compound is then accumulated continuously as monomolecular (outer) layer on the pre-liposomes, in order to form the finished liposomes provided with a bilayer. In this case, the twoat least monomolecular (inner and outer) layers of the at least one first amphiphilic compound and the at least one second amphiphilic compound approach one another initially with sufficient proximity that they form the finished bilayer with virtually freely adjustable composition of the inner and outer faces on account of interactions, especially hydrophobic interactions, between the same first and second amphiphilic compounds of the monolayers (if symmetric liposomes are to be generated) or especially different first and second amphiphilic compounds of the monolayers (if asymmetric liposomes are to be generated). Then the encapsulated droplets of the active ingredient solution from the first emulsion, on account of the difference in density, are pressed against the newly formed bilayer with sufficient force that the latter is deformed and ultimately surrounds the entire active ingredient solution droplet, which ultimately becomes divided from the bilayer and breaks away from the phase boundary, such that the envelope with a bilayer transforms the active ingredient solution droplet into a liposome. If the at least one second amphiphilic compound used here is a compound other than the at least one first amphiphilic compound, it is possible in a simple manner to generate asymmetric liposomes having a different structure of the inner and outer, at least monomolecular layer of their bilayer.
For the sake of completeness, it should be pointed out here that what is being addressed by “of zero or sparing miscibility” in relation to the (hydrophilic) active ingredient solution with the (hydrophobic) first liquid of the first emulsion or in relation to the (hydrophobic) first liquid of the first emulsion with the mixture of the (hydrophilic) liquid phase and the at least one second amphiphilic compound is sufficiently sparing miscibility that leads to formation of a phase boundary on combination of the aforementioned components.
In an advantageous configuration of the method of the invention, it may be the case that
Rather than a mere mixture or suspension of the at least one second amphiphilic compound with the liquid phase from step (c), what is used is consequently a second emulsion, in that the liquid phase, especially the hydrophilic liquid phase, of this mixture is emulsified with the at least one second liquid, especially hydrophobic second liquid, of zero or sparing miscibility therewith. In this way, it is possible to produce emulsion droplets in the second emulsion—in a corresponding manner to that in the first emulsion—formed from the droplets of the second liquid emulsified in the liquid phase and an at least monomolecular layer of the at least one second amphiphilic compound accumulated thereon. The at least one second amphiphilic compound may be immobilized in this way and is consequently no more or less free in a mere mixture or suspension. If this second emulsion is contacted in step (d) with the first emulsion to form a phase boundary between the continuous phases thereof, the subsequent centrifugation of the first and second emulsions according to step (e) transfers the droplets of the second liquid with the at least monomolecular layer of the at least one second amphiphilic compound accumulated thereon from the liquid phase of the second emulsion constantly to the phase boundary between the first emulsion and the second emulsion, such that excessive enrichment of the at least one second amphiphilic compound, let alone the formation of an organogel therefrom, at the phase boundary can be reliably avoided because the at least one second amphiphilic compound immobilized on the emulsified droplets of the second liquid is supplied constantly to the phase boundary in this way and is continuously enriched at this phase boundary, in order to accumulate on the inner layer when the droplets of the active ingredient solution with the at least monomolecular (inner) layer of the at least one first amphiphilic compound accumulated thereon pass through the phase boundary as the outer layer and to form the bilayer. Furthermore, it is especially possible in this way to keep the first and second amphiphilic compounds ideally separated from one another (each amphiphilic compound is accumulated and consequently immobilized on emulsion droplets of the first and second emulsions that are in contact with one another solely via the phase boundary), such that highly asymmetric liposomes can be produced if the second amphiphilic compound(s) is/are chosen differently than the first amphiphilic compound(s).
The droplets of the second liquid, especially the hydrophobic second liquid, emulsified in the second emulsion and having the at least monomolecular layer of the at least one second amphiphilic compound accumulated thereon are also referred to hereinafter, for the sake of simplicity, as “amphiphile carriers”.
In an advantageous configuration of the method of the invention, it may furthermore be the case that the first liquid of the first emulsion according to step (b) above is chosen in such a way that the solubility of the at least one first amphiphilic compound in the first liquid is not more than about 1×10−5 mol/l, preferably not more than about 0.5×10−5 mol/l, most preferably not more than about 1×10−6 mol/l, especially not more than about 1×10−7 mol/l. In this connection, it has been found that, surprisingly, enrichment of the at least one first amphiphilic compound at the phase boundary between the first emulsion and the liquid phase of the mixture with the at least one second amphiphilic compound, or the second emulsion, which is in contact therewith, let alone the formation of an organogel, as can be found to be problematic in the prior art outlined at the outset, can be effectively minimized by choosing the first liquid of the first emulsion in the above step (b) such that the solubility of the at least one first amphiphilic compound in the first liquid is very low. In this way, it is possible not to form a “barrier layer” of the at least one first amphiphilic compound of the phase boundary that could block the phase boundary and hence prevent the formation of liposomes from the active ingredient solution droplets on passage through the phase boundary, such that a high yield of liposomes provided with a bilayer of the at least one first and second amphiphilic compound is obtained and the efficiency of the process is considerably improved in that the active ingredient solution waste, which should be recovered for economic reasons, is minimized.
In order also to prevent excessive enrichment of the at least one second amphiphilic compound at the aforementioned phase boundary and consequently the formation of an organogel therefrom, it may additionally be a particular option for the first liquid of the first emulsion in step (b) to be chosen such that the solubility both of the at least one first amphiphilic compound and of the at least one second amphiphilic compound in the first liquid is not more than about 1×10−5 mol/l, preferably not more than about 0.5×10−5 mol/l, most preferably not more than about 1×10−6 mol/l, especially not more than about 1×10−7 mol/l.
The second liquid, especially the hydrophobic second liquid, which has zero or sparing miscibility with the liquid phase, especially the hydrophilic liquid phase, of the second emulsion, which forms the “core” of the amphiphile carrier in the context of the present disclosure with the at least one second amphiphilic compound accumulated thereon, may advantageously be chosen to correspond to the first liquid, especially the hydrophobic first liquid, of the first emulsion from step (b) that forms the continuous phase of the first emulsion. If the first liquid, accordingly, corresponds to the second liquid, this can be recovered without difficulty in a composition that is always constant after, during the centrifuging, the amphiphile carrier from the second liquid and the at least one second amphiphilic compound accumulated thereon have reached the phase boundary between the first emulsion and the second emulsion, the second amphiphilic compound is enriched at the phase boundary and has been accumulated as outer layer on the pre-liposomes composed of the active ingredient solution with the at least one first amphiphilic compound accumulated thereon, and the droplets of the second liquid of the second emulsion have been transferred through the phase boundary into the same first liquid.
The mixture in the form of the second emulsion with the at least one second amphiphilic compound immobilized on the amphiphile carriers according to step (c) may preferably be produced by
In a corresponding manner, the first emulsion can advantageously be produced in step (b) by first
As already mentioned, in the selection of the first liquid, especially the hydrophobic first liquid, of the first emulsion from step (b), it may prove advantageous if the solubility of the at least one first amphiphilic compound and preferably also of the at least one second amphiphilic compound should be not more than about 1×10−5 mol/l, such that the formation of organogel structures at the phase boundary between the first liquid of the first emulsion and the mixture of the liquid phase, especially the hydrophilic liquid phase, and the at least one second amphiphilic compound or the second emulsion is reliably avoided. If the active ingredient solution has a hydrophilic character and is in aqueous and/or alcoholic form, for example, the first liquid used in the first emulsion in step (b) may appropriately be a hydrophobic liquid especially selected from the group of the
where each “R” is a hydrogen atom or an alkyl group and “n” is a natural number.
It is consequently not only in this case that the solvent used in the active ingredient solution in step (a) is preferably a hydrophilic solvent, especially a water-based hydrophilic solvent, including water, for example isotonic solutions, and/or an alcohol-based hydrophilic solvent, especially based on ethanol or glycerin, or glycerol.
In one development of the method of the invention, it may be the case that
It is advantageously an option here for the active ingredient solution, especially the hydrophilic active ingredient solution, having the at least monomolecular inner layer of the at least one first amphiphilic compound accumulated thereon to be kept in the solid state during the centrifuging, in order to move it, on account of a resultant difference in density, from the phase boundary in the direction of the mixture or of the second emulsion.
Consequently, the first emulsion is preferably cooled to such a degree as to freeze the active ingredient solution droplets, for example aqueous and/or alcoholic active ingredient solution droplets, but to leave the first liquid, especially the hydrophobic first liquid, surrounding it, i.e. the continuous phase of the first emulsion, still in liquid form. This may contribute to prevention of excessive or even complete deformation of the active ingredient solution droplets with the (inner) at least monomolecular layer of the at least one first amphiphilic compound accumulated thereon, without penetrating the phase boundary and hence in turn forming a barrier layer at the phase boundary. If, more particularly, a first liquid, especially a hydrophobic first liquid, which is heavier than the aqueous and/or alcoholic active ingredient solution droplets, for example, is chosen, the frozen active ingredient solution droplets, as a result of centrifugal forces, not only move more easily or quickly within the first liquid of the first emulsion but also in the liquid phase, especially the hydrophilic liquid phase, of the mixture with the at least one second amphiphilic compound or in the second emulsion, after they have passed through the phase boundary and the enveloping with a bilayer to form the finished liposomes has taken place, because the frozen active ingredient solution droplets have a lower density than the unfrozen liquid phase, especially the hydrophilic unfrozen liquid phase of the mixture with the at least one second amphiphilic compound or of the second emulsion, where this liquid phase, just like the solvent of the active ingredient solution droplets, may especially be in aqueous or alcoholic form or in the form of water. For this purpose, at least the first emulsion should be at a temperature slightly below the freezing point of the active ingredient solution droplets in order that they remain frozen in the surrounding first liquid of the first emulsion.
As already mentioned, the active ingredients incorporated into a liposome are protected against the destructive effect of enzymes and from premature secretion from the body by the bilayer composed of amphiphilic compounds, such as in the form of a bilayer composed of the same or especially of different lipids, on the way to its intended site in the organism. In some pharmaceutical products, however, the liposomes must also be protected by a surface polymer layer, typically formed on the basis of polyethylene glycol (PEG), in order to prevent opsonization and phagocytosis by immune cells, for example in the liver, before the active ingredient has reached its intended site. With the aid of extraneous molecules, for example antibodies, that become attached to the outside of the liposomes, it is additionally possible to attempt to precisely determine the intended site of the active ingredient by binding to a specific receptor (“drug targeting”). It is thought here that liposomes, on account of their cell membrane-like chemical characteristics, are fused relatively easily to the cell membrane or, after pinocytosis or endocytosis, to the endosomal and lysosomal membrane, and then release their contents into the cell interior. Accordingly, in an advantageous development of the method of the invention, it may be a case that the bilayer consisting of the two at least monomolecular layers of the at least one first amphiphilic compound and of the at least one second amphiphilic compound,
especially exclusively the outer at least monomolecular layer of the at least one second amphiphilic compound, is modified by reaction with hydrophilic polymer conjugates. This may firstly be accomplished, for example, in that the ready-produced liposome is modified by such polymer conjugates being accumulated on the at least monomolecular outer layer of the bilayer, for example by means of electrostatic charging thereof, as is known as such from the prior art. Furthermore, especially the method of the invention opens up the option of using at least one second amphiphilic compound, for example in the form of lipids, to which the polymer conjugates have already been bound beforehand for the at least monomolecular outer layer of the bilayer.
Furthermore, the method of the invention may be performed
In the case of batchwise performance of the method in a batchwise centrifugation device, it may be the case, for example, that firstly the first emulsion from step (b) and secondly the mixture or second emulsion from step (c) is supplied to the centrifugation device, and then centrifuged, after which firstly the liquid phase comprising the liposomes provided with the bilayer composed of the two at least monomolecular layers of the at least one first amphiphilic compound and the at least one second amphiphilic compound and secondly the first liquid are withdrawn from the centrifugation device. In the case of semicontinuous performance of the method in a batchwise centrifugation device, it may be the case that, for example, firstly the first emulsion from step (b) and secondly the mixture or second emulsion from step (c) are supplied continuously to the centrifugation device over a period of time, during which they are centrifuged, and then firstly the liquid phase comprising the liposomes provided with the bilayer composed of the two at least monomolecular layers of the at least one first amphiphilic compound and the at least one second amphiphilic compound and secondly the first liquid are withdrawn from the centrifugation device.
As also elucidated in detail further down with reference to
It may preferably be the case here that
In addition, it may be advantageous in this context when
Further features and advantages of the invention will be apparent from the description that follows of a working example with reference to the drawings. The drawings show:
As is additionally apparent in the right-hand upper section of
As apparent from the bottom left-hand section of
As is additionally apparent from all four sections of
Step (e) of the method finally envisages centrifugation of the first emulsion 4 and the second emulsion 9 that are in contact with one another via the phase boundary 6, in order firstly to transfer the droplets of the active ingredient solution 1 present in the first emulsion 4 with the monomolecular inner layer of the first amphiphilic compound 2 accumulated thereon in the direction of the arrows P1 from the first liquid 3 of the first emulsion 4 through the phase boundary 6 with the molecules of the second amphiphilic compound 8 enriched thereon into the liquid continuous phase of the second emulsion 9, wherein, when it passes through the phase boundary 6, the second amphiphilic compound 8 is accumulated on the monomolecular inner layer of the first amphiphilic compound 2 of the droplets of the active ingredient solution 1 to form a further monomolecular—outer—layer thereof, in order to produce the bilayer composed of two monomolecular layers, namely firstly of the first amphiphilic compound 2 (inner layer) and secondly of the second amphiphilic compound 8 (outer layer), i.e. in order to form the finished liposomes L from the pre-liposomes M. The outer layer of the bilayer of the second amphiphilic compound 8 has an opposite orientation from the inner layer composed of the first amphiphilic compound 2, i.e. the nonpolar regions of the second amphiphilic compound 8 of the outer layer point in the direction of the polar regions of the first amphiphilic compound 2 of the inner layer, i.e. in the direction of the hydrophilic active ingredient solution 1 now encapsulated in a liposome L, while the polar regions of the second amphiphilic compound 8 of the outer layer of the bilayer point in the direction of the—hydrophilic—liquid phase of the second emulsion 9 surrounding the liposome L (in this regard see the two upper sections of
Since the pre-liposomes M composed of the droplets of the (hydrophilic) active ingredient solution 1 with the inner layer of the first amphiphilic compound 2 accumulated thereon have a lower density than the surrounding (hydrophobic) first liquid 3 of the first emulsion 4, they experience a force acting in the direction of the arrows P1 in the centrifugal field, which accelerates them in centripetal direction and brings them to the phase boundary 6 covered with a very substantially monomolecular layer of the second amphiphilic compound 8. The pre-liposome M is pushed against this layer of the second amphiphilic compound 8 enriched at the phase boundary 6 with such a force that the monomolecular layer of the second amphiphilic compound 8 enriched at the phase boundary 6 adjoins the inner layer of the first amphiphilic compound 2 accumulated on the droplets of the active ingredient solution 1, and hydrophobic interaction of two monomolecular layers gives rise to the bilayer of the finished liposome L, which, after further movement in the direction of the arrows P1, is dispersed in the (hydrophilic) liquid phase of the second emulsion 9 in the centrifugal field. In the case of a primarily ball-shaped or spherical shell, the droplets of the active ingredient solution 1 or the pre-liposome M consequently forms a liposome L having, in the bilayer that forms its membrane, firstly an inner layer of the first amphiphilic compound 2 and secondly an outer layer of the second amphiphilic compound 8. Consequently, it is firstly possible to produce symmetric liposomes L when the first amphiphilic compound 2 is chosen to correspond to the second amphiphilic compound 8; secondly, it is especially possible to produce asymmetric liposomes L when the first amphiphilic compound 2 is chosen differently than the second amphiphilic compound 8. At the same time, it should be pointed out here that, rather than a (single) first amphiphilic compound 2 and/or second amphiphilic compound 8, it is of course also possible to use a mixture of such compounds, for example a mixture of multiple lipids, which then respectively form the inner and outer layers of the bilayer of the liposome L (in this regard likewise see the two upper sections of
As also apparent from the two lower sections of
Since the amphiphile carriers M′ composed of the droplets of the (hydrophobic) second liquid 10 with the second amphiphilic compound 8 accumulated thereon have a higher density than the surrounding (hydrophilic) liquid phase of the second emulsion 9, they experience a force that acts in the direction of the arrows P2 in the centrifugal field, which accelerates them in centrifugal direction and brings them to the phase boundary 6. As they do so, they shed their monomolecular layer of the second amphiphilic compound 8 as they pass into the first (hydrophobic) liquid 3, i.e. into the continuous phase of the first emulsion 4, at the phase boundary 6. In this way, the layer of the second amphiphilic compound 8 deposited at the phase boundary 6 is constantly renewed as a result of the phase transfer of the droplets of the second (hydrophobic) liquid, or the second amphiphilic compound 8 is continuously “replenished” at the phase boundary 6, whereas it is continuously consumed as the outer layer by the above-described accumulation to form the liposomes L.
After more or less complete formation of the liposomes L or after more or less complete consumption of the amphiphile carriers M′ composed of the second liquid 10 with the second amphiphilic compound 8 accumulated thereon that were originally provided in the second emulsion 9, it is ultimately possible to separate the liquid phase in which the liposomes L are dispersed from the first liquid 3, which is possible in a simple manner because the miscibility of the hydrophilic liquid phase with the hydrophobic first liquid 3 is very sparing at most, and because of their different density.
For the reasons mentioned above, it is additionally possible that the first emulsion 4 is cooled down to a temperature between the melting point of the (hydrophobic) first liquid 3 and the melting point of the (hydrophilic) active ingredient solution 1, in order to convert the active ingredient solution 1 of the droplets having the monomolecular inner layer of the first amphiphilic compound 2 accumulated thereon that are emulsified in the first liquid 3 into the solid state, after which the first emulsion 4 in the solid state of the droplets of the active ingredient solution 1 is contacted with the (hydrophilic) liquid phase of the second emulsion 9, i.e. with the continuous phase thereof, to form the phase boundary 6, and the first emulsion 4 and the second emulsion 9 that are in contact with one another via the phase boundary 6 are centrifuged, especially keeping the droplets of the active ingredient solution 1 constantly in the solid state of matter.
In addition, it is firstly possible if required to modify the finished liposomes L with polymer conjugates, for example those based on polyethylene glycol (PEG), for example by attaching them electrostatically to the second amphiphilic compound 8 of the outer layer of the bilayer (not shown). Secondly, the method of the invention especially offers the option that the second amphiphilic compound 8 used, which forms the monomolecular outer layer of the bilayer of liposomes L, is one or more lipids, where polymer conjugates have already been attached beforehand to at least some molecules of these lipids.
While the embodiment of a method of the invention for encapsulation of active ingredients in symmetric or especially asymmetric liposomes L that has been elucidated above with reference to
On its right-hand side in
In the centrifuge chamber 13, in the region of the section on the outlet side thereof, but upstream of the outflow weir 19, there is also a first retaining weir 20 that extends essentially in radial direction thereof, which is configured essentially in the form of a circular ring, for example, and extends from the outer circumferential wall 12 of the centrifuge device inward by a radial distance R1, where this radial distance R1, i.e. the radial width of the first retaining weir 20, appropriately corresponds at least to the radial width of the passage orifice between the radially outer end of the outlet weir 19 and the circumferential wall 12, or preferably at least slightly exceeds it. In addition, in the centrifuge chamber 13, in the region of the section on the outlet side thereof, but once again upstream of the outlet weir 19, there is a second retaining weir 21 that likewise extends essentially in radial direction thereof, which, in the present case, is in the form of a circular ring, for example, and extends between a section of the centrifuge chamber 13 which is roughly in the middle in radial terms to close to the central axis of rotation 11 of the centrifuge device, although it may instead also be of essentially circular configuration and consequently may have no central passages (not shown). The radial distance R2 of the second retaining weir from the central axis of rotation 11, i.e. the radial width of the second retaining weir 21, appropriately corresponds at least to the radial width of the passage orifice between the radially inner end of the outlet weir 19 and the central axis of rotation 11, or preferably exceeds it at least slightly.
For continuous performance of the embodiment of the method of the invention described above with reference to
In the centrifuge chamber 13, through which the flow direction is axial, i.e. from left to right in
In addition, the droplets of the hydrophobic second liquid 10 that are emulsified in the hydrophilic liquid phase of the second emulsion 9 and have been provided with a monomolecular layer of the second amphiphilic compound 8 may be accumulated in the centrifuge chamber 13 by means of the second retaining weir 21. One function of the second retaining weir 21 is consequently primarily that the emulsified droplets of the second hydrophobic liquid 10 provided with a monolayer of the second amphiphilic compound 8 in the second emulsion 9 which has not yet separated out the second amphiphilic compound 8 at the phase boundary 6 are not entrained out of the centrifuge chamber 13 via the second outlet 18, but reach the phase boundary 6 beforehand by virtue of the centrifugal forces induced, in order to very substantially enrich the second amphiphilic compound 8 there.
Finally, firstly the continuous hydrophilic liquid phase of the second emulsion 9 comprising the liposomes L provided with the bilayer composed of the first amphiphilic compound 2 (inner layer) and the second amphiphilic compound 8 (outer layer) (cf.
The invention is elucidated in detail hereinafter by working examples.
The hydrophilic liquid phase (15 mM PP in water) with the finished liposomes suspended therein is analyzed by means of photon correlation spectroscopy (PCS, Malvern Zetasizer Nano ZS90) in order to determine the size of the liposomes produced in this way. The liposomes produced in this way, which have a different composition of the inner and outer monomolecular layer of their bilayer, have a particle size of 367 nm±61 nm, a particle count of 3207±52 kcounts/s (derived count rate) and a polydispersity index (PDI) of 0.30.
The hydrophilic liquid phase (150 mM PP in water) with the finished asymmetric liposomes suspended therein is analyzed by means of photon correlation spectroscopy analogously to example 1, in order to determine the average size of the liposomes produced thereby. The liposomes produced in this way, which have a different composition of the inner and outer monomolecular layer of their bilayer, have a particle size of 221 nm±2 nm, a particle count of 97 kcounts/s±2 kcounts/s (derived count rate) and a polydispersity index (PDI) of 0.05.
Evidence of Asymmetry:
The liposomes produced according to the above working example 2 were analyzed as follows with regard to the degree of asymmetry of the first and second monomolecular layers of their bilayer:
Example 2 was conducted firstly in the above manner, except that 1 mol % of a marker substance (N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)-1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine, NBD-PE), had been added solely to the first amphiphilic compound DPPC intended for the inner monomolecular layer. Secondly, example 2 was conducted in the above manner, except that 1 mol % of the marker substance (NBD-PE) had been added solely to the second amphiphilic compound DSPC intended for the outer monomolecular layer.
In order to determine the distribution of the NBD-PE labeling substance in the inner or outer layer of the bilayer of the liposomes produced according to working example 2, in addition, in the two experiments in which firstly only the inner monomolecular layer (“I”) of the first amphiphilic compound (DPPC) and secondly only the outer monomolecular layer (“A”) of the second amphiphilic compound (DSPC) had been labeled with the NBD-PE labeling substance, firstly the NBD-PE incorporated in the outer layer of the bilayer was destroyed in each case by addition of sodium dithionite in order to extinguish the signal emitted by NBD-PE in the outer layer of the bilayer of the liposomes. By later addition of Octoxinol 9 (“Triton-X detergent”™), the membrane of the liposomes was rendered permeable in each case, such that the sodium dithionite also penetrates into the inner layer of the bilayer and also destroys the NBD-PE incorporated therein, in order also to extinguish the signal emitted by NBD-PE in the inner layer of the bilayer of the liposomes.
Whereas
| Number | Date | Country | Kind |
|---|---|---|---|
| 10 2018 006 439.6 | Aug 2018 | DE | national |
| Filing Document | Filing Date | Country | Kind |
|---|---|---|---|
| PCT/EP2019/000132 | 4/29/2019 | WO | 00 |
