Claims
- 1. A method of doing business, comprising the steps of:
using a 13C labeled molecule to determine information on how carbon atoms of the labeled molecule are repositioned when acted on in a living system; analyzing the determined information and applying the analysis to select a lead compound for clinical trials; conducting clinical trials on the selected lead compound; and marketing the lead compound as a pharmaceutical drug after completing the clinical trials and obtaining governmental approval.
- 2. The method of claim 1, wherein the living system comprises a living cell.
- 3. The method of claim 1, wherein the living system comprises a plurality of living cells in a cell culture.
- 4. The method of claim 1, wherein the living system comprises living tissue.
- 5. The method of claim 1, wherein the living system comprises a multi-cellular organism.
- 6. The method of claim 1, wherein the 13C labeled molecule is 13C labeled glucose.
- 7. The method of claim 6, wherein the 13C labeled glucose is chosen from [1,2-13C2] glucose, [1,2,5,6-13C4] glucose and [5,6-13C2] glucose.
- 8. The method of claim 1, wherein the 13C labeled molecule is [1,2-13C2] glucose and information is determined by comparing data obtained when the living system is in contact with a candidate compound to data obtained when the living system is not in contact with the candidate compound.
- 9. The method of claim 1, wherein the determining information comprises determining molecular weight of 13C labeled molecules acted on by the living system.
- 10. The method of claim 8, wherein the determining information comprises determining positions of at least two carbon atoms of 13C labeled molecules acted on by the living system and tracking changes in those positions as the labeled molecule moves through a metabolic pathway.
- 11. The method of claim 8, wherein the determined information is analyzed to obtain data on how the candidate compound changes metabolic pathways of the living system involved in assembling components of a new living system.
- 12. The method of claim 1, wherein information is determined on molecular weight and 13C labeled carbon positions of the 13C labeled molecule acted on by both a control living system and a living system acted on by a candidate compound.
- 13. The method of claim 1, wherein the using of a 13C labeled molecule to determine information and the analyzing of the determined information is carried out by
adding 13C labeled molecules to a system which labels change the molecular weight of molecules in the system which incorporated the 13C label; analyzing molecules in the system to determine changes to molecular weights; analyzing molecules in the system to determine positions of 12C and 13C carbons; comparing determined changes to molecular weights and 13C carbon positions in a control versus a compound treated system.
- 14. The method of claim 13, wherein the comparing is carried out to reveal effects of the compound on a specific metabolic pathway of the system; and
wherein the system is comprised of organisms chosen from cells and viruses which system is producing new organisms and the metabolic pathway is one which is involved in production of the new organisms; and wherein the 13C labeled molecules are 13C labeled glucose chosen from [1,2-13C2] glucose, [1,2,5,6-13C4]glucose, [5,6-13C2]glucose.
- 15. The method of claim 13, wherein the system is chosen from a plurality of living cells in a cell culture, living tissue, a multi-cellular organism, bacteria cells, plant cells, bacteria hosting phages, cells hosting viruses and cells hosting virus particles.
- 16. The method of claim 13, further comprising:
separating from the system molecules which have incorporated 13C label wherein the separating is carried out by a means chosen from centrifugation, physical/chemical purification, chemical derivatization, liquid chromatography and gas chromatography; and wherein the analyzing is carried out by spectrometry selected from mass spectrometry and nuclear magnetic resonance.
- 17. A method of doing business, comprising the steps of:
determining a metabolic step involved in the formation of a glucose based metabolite of a living organism by: adding 13C labeled glucose to a living cellular test system in the presence of a test compound; allowing the test compound to interact with the test system for a given period of time under known conditions; separating 13C labeled molecules away from the test system; analyzing the separated 13C labeled molecules; comparing analysis results obtained from the test system with results obtained from a known system; using results of the comparing to determine if the test compound should be tested further; conducting further testing on the test compound; and marketing the test compound.
- 18. The method of claim 17, wherein the living cellular system is chosen from a known cell culture grown on known cellular nutrients, a transgenic mouse, a genetically normal wild type animal, and a human.
- 19. A method, comprising the steps of:
adding 13C labeled molecules to a test system which test system acts on the molecules and which 13C label changes molecule weights of molecules which the label is added to; analyzing 13C labeled molecules in the system after the system has acted on the 13C labeled molecule added to the system and obtaining information from the analyzing; comparing the information obtained with information on a known system.
- 20. The method of claim 19, wherein the known system is a control system substantially identical to the test system except for a compound added to the test system.
- 21. A method of determining the specific metabolic steps involved in the formation of a glucose based metabolite of a living organism, comprising the steps of:
adding 13C labeled glucose to a living cellular system; allowing the system to act on the labeled glucose for a given period of time under known conditions; separating 13C labeled molecules away from the system; analyzing the separated 13C labeled molecules; and comparing analysis results to analysis results obtained from a known system.
- 22. A method, comprising the step of:
adding 13C labeled molecules to a system which 13C label replaces 12C in molecules increasing the molecular weight of the molecules where the 13C replaces a 12C; analyzing molecules incorporating 13C labels to determine changes to molecular weights relative to when the molecule was comprised of 12C; analyzing 13C labeled molecules to determine the positions of 12C and 13C labeled carbons; comparing determined changes to molecular weights and 13C labeled carbon positions in control versus a drug treated system in order to reveal specific drug action on molecules of the system.
- 23. The method of claim 22, wherein the comparing is a comparing of changes to molecular weights and 13C labeled carbon positions in an organism chosen from a bacteria, cell, virus and phage to reveal metabolic pathways involved in the assembly of a progeny of the organism.
- 24. A method, comprising the steps of:
labeling precursor molecules with a 13C isotope at a known position; adding the labeled precursor molecules to a changing test system; analyzing molecules in the test system which molecules have incorporated the 13C label, wherein the analyzing is carried out at a first point in time; comparing information obtained from the analyzing with information chosen from a control system information and reference information.
- 25. The method of claim 24, wherein the comparing of information is used to determine how a metabolic pathway of the test system is changed relative to the control system information or reference information.
- 26. The method of claim 24 further comprising:
analyzing molecules in the test system which have incorporated the 13C label at a second point in time after the first point in time.
CROSS-REFERENCE
[0001] This application claims the benefit of U.S. Provisional Application No. 60/367,142, filed Mar. 22, 2002, which application is incorporated herein by reference.
GOVERNMENT RIGHTS
[0002] This invention was made with government support under federal grant PO 1 CA42710-15 awarded by the National Institutes of Health to the University of California at Los Angeles (UCLA) Clinical Nutrition Research Unite (CNRU). The United States Government may have certain rights in this invention. This grant was awarded based on a competitive peer review in order to support academic research in the stable isotope core laboratory of this CNRU.
Provisional Applications (1)
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Number |
Date |
Country |
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60367142 |
Mar 2002 |
US |