Claims
- 1. A method of maturing cotyledonary stage pine embryos on a medium, comprising (a) distributing embryogenic plant material onto a solid medium in a vessel, (b) placing a filter over the vessel containing the embryogenic plant material wherein said filter allows a controlled rate of water loss and a controlled rate of exchange of oxygen and is impermeable to microorganisms.
- 2. A method as claimed in claim 1 wherein said maturing cotyledonary stage pine embryos are Pinus radiata embryos.
- 3. A method as claimed in claim 1 wherein the filter is a film.
- 4. A method as claimed in claim 1 wherein the filter allows the transmission of water vapor at the rate of between 90 to 150 grams/square meter/day.
- 5. A method as claimed in claim 4 wherein the filter has a water vapor transmission rate of 118 grams/square meter/day.
- 6. A method as claimed in claim 4 wherein the filter allows oxygen to be exchanged at the rate of between 7200 and 32000 cubic centimeters/square meter/day.
- 7. A method as claimed in claim 6 wherein the filter allows the rate of oxygen exchange in the order of 16700 cubic centimeters/square meter/day.
- 8. A method of growing plant material on a medium as claimed in claim 1 and including the further step of controlling the atmosphere outside of the filer.
Priority Claims (1)
Number |
Date |
Country |
Kind |
241054 |
Dec 1991 |
NZX |
|
Parent Case Info
This application is a continuation application under 37 C.F.R. 1.62 of prior application Ser. No. 07/991,994, filed on Dec. 17, 1992 now abandoned.
US Referenced Citations (2)
Continuations (1)
|
Number |
Date |
Country |
Parent |
991994 |
Dec 1992 |
|