Claims
- 1. A method of producing a transgenic livestock animal, said method comprising:
(a) introducing a nucleoprotein made up of nucleic acid and a recombinase into a totipotent or pluripotent cell to produce a recombinant totipotent or pluripotent cell; and (b) growing said recombinant totipotent or pluripotent cell to produce said transgenic, non-human animal.
- 2. The method according to claim 1, wherein said livestock animal is an ungulate.
- 3. The method according to claim 2, wherein said ungulate is chosen from pigs, goats, sheep, cows and horses.
- 4. The method according to claim 3, wherein said livestock animal is chosen from goats and pigs.
- 5. The method according to claim 1, wherein said nucleic acid of said nucleoprotein includes a homology clamp.
- 6. The method according to claim 1, wherein said nucleic acid of said nucleoprotein does not include a homology clamp.
- 7. The method according to claim 1, wherein said growing step (b) comprises introducing said recombinant totipotent or pluripotent cell into a pseudopregnant non-human animal so that said cell develops into said transgenic, non-human animal.
- 8. The method according to claim 1, wherein said method has an efficiency of generation of for said transgenic non-human animal that is at least two-fold higher as compared to the efficiency of generation of said transgenic non-human animal in the absence of a recombinase.
- 9. The method according to claim 1, wherein said nucleic acid of said nucleoprotein is a double-stranded deoxyribonucleic acid.
- 10. The method according to claim 1, wherein said nucleic acid of said nucleoprotein is a single-stranded deoxyribonucleic acid.
- 11. The method according to claim 1, wherein said recombinase of said nucleoprotein is a RecA protein.
- 12. The method according to claim 1, wherein said recombinase of said nucleoprotein is a RAD51 protein.
- 13. The method according to claim 1, wherein said introducing step (a) comprises pronuclear microinjection of said nucleoprotein into a fertilized pronuclear-stage embryo.
- 14. A transgenic animal produced according to the method of claim 1.
- 15. A nucleoprotein comprising a recombinase and a nucleic acid, wherein said nucleic acid includes a homology clamp for a livestock genome.
- 16. A kit for generating a transgenic, non-human animal, said kit comprising:
a recombinase; and instructions for practicing the method of claim 1.
- 17. The kit according to claim 16, wherein said recombinase is a RecA recombinase.
- 18. The kit according to claim 16, wherein said recombinase is a Rad51 recombinase.
- 19. The kit according to claim 16, wherein said kit further comprises a buffer.
- 20. The kit according to claim 16, wherein said kit further comprises a microinjection device.
CROSS-REFERENCE TO RELATED APPLICATIONS
[0001] Pursuant to 35 U.S.C. §119 (e), this application claims priority to the filing date of the United States Provisional Patent Application Serial No.: (a) 60/317,915 filed Sep. 7, 2001; the disclosure of which are herein incorporated by reference.
Provisional Applications (1)
|
Number |
Date |
Country |
|
60317915 |
Sep 2001 |
US |