Claims
- 1. A method of screening a hair follicle for growth by observing label-retaining cells located in the bulge region comprising:
- (a) isolating a hair follicle
- (b) inducing hair follicle cells into a proliferative phase;
- (c) continuously labeling said cells with a selected radioisotope for a selected period;
- (d) collecting label-retaining cells from the bulge region located at the midportion of a hair follicle at the arrector pili muscle attachment site; and
- (e) observing said bulge cells for labeling to determine the number and viability of said bulge cells relative to established controls.
- 2. The method of claim 1 wherein the cells are continuously labeled for at least 7 days.
- 3. The method of claim 2 wherein the cells are continuously labeled for 14 days.
- 4. The method of claim 1 wherein the cells are induced into a proliferative phase in response to hyperplastic stimuli.
- 5. The method of claim 4 wherein the cells are induced into a proliferative phase by exposure to TPA or a cytokine.
- 6. The method of claim 1, following inducing said bulge cells into a proliferative phase, further comprising exposing said induced cells to colcemide and then observing the cells for labeled mitotic figures.
Parent Case Info
This application is a continuation of application Ser. No. 07/971,687, filed Nov. 4, 1992, and now U.S. Pat. No. 5,279,969, issued Jan. 18, 1994, which is a continuation of application Ser. No. 07/676,185, filed Mar. 27, 1991, now abandoned.
Government Interests
This invention was made in the course of work supported by NIH grants AR39674, EY06769, AR34511 and EY4722. The Government has certain rights in this invention.
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Kind |
4806476 |
Coons et al. |
Feb 1989 |
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5229271 |
Philpott |
Jul 1993 |
|
Non-Patent Literature Citations (3)
Entry |
Cotsarelis et al., An Improved Method for Detection of Epithelial Stem Cells, J. Invest. Dermol., 92(3), 1989a. |
Cotsarelis et al., Existence of Slow-Cycling Limbal Epithelial Basal Cells That Can be Preferentially Stimulated to Proliferate: Implications on Epithelial Stem Cells, Cell, 57:201-209 1989b. |
Cotsarelis et al., Label-Retaining Cells Reside in the Bulge Area of Pilosebaceous Unit: Implications for Follicular Stem Cells, Hair Cycle, and Skin Carcinogenesis, Cell, 61:1329-1337 1990. |
Continuations (2)
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Number |
Date |
Country |
Parent |
971687 |
Nov 1992 |
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Parent |
676185 |
Mar 1991 |
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