Claims
- 1. A method of inhibiting vertebrate collagenase activity comprising treating a vertebrate collagenase-containing substrate with a collagenase inhibitory effective amount of a peptide hydroxamic acid derivative having the following structural formula:
- R--Pro--Leu--Gly--NHOH
- wherein R=H or N-protecting group or agarose.
- 2. The method of claim 1 in which the collagenase-containing substrate is human synovial collagenase.
- 3. The method of claim 1 in which the collagenase-containing substrate is human skin fibroblast collagenase.
- 4. A method of isolating vertebrate collagenase comprising treating a vertebrate collagenase-containing substrate with an affinity resin comprising agarose covalently coupled to the peptide hydroxamic acid derivative Pro--Leu--Gly--NHOH.
Parent Case Info
This is a division of application Ser. No. 789,271, filed Oct. 18, 1985, and now U.S. Pat. No. 4,687,841.
US Referenced Citations (4)
Non-Patent Literature Citations (3)
Entry |
Masui et al., Biochem. Med., 17, 215-221, (1977). |
Nishino and Powers, Biochem., 17, 2846, (1978). |
Nishino and Powers, Biochem., 18, 4340-4347, (1979). |
Divisions (1)
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Number |
Date |
Country |
Parent |
789271 |
Oct 1985 |
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