Claims
- 1. A method of manufacturing a device for assay of a liquid sample having:a support composed of an organic macromolecule, said support having a surface divided into at least two areas located adjacent to each other; a divider on the surface defining a border between said two areas to separate the areas into a first area and a second area; a detection layer affixed the first and containing a reagent; and a water-swelling layer affixed to the second area, said water-swelling layer being capable of expanding by absorbing water when a water-containing liquid sample is applied to the water-swelling layer to extend a part of the water-swelling layer over the divider and into contact with the detection layer, said divider preventing the water-swelling layer from completely covering the detection layer so that a portion of the detection layer remains exposed, the liquid sample moving by capilarity from the water-swelling layer to the detection layer where it reacts with the reagent, thereby permitting the reaction in the detection layer to be identified optically, said method comprising the steps of: (a) reforming a perimeter of the first area on the surface of the support so as to render the perimeter hydrophilic; (b) forming the divider composed of a water-repellent material on the reformed perimeter; (c) reforming the first area and at least a portion of the second area adjacent to the divider so as to render them hydrophilic; and (d) affixing the detection layer and the water-swelling layer to the reformed first area and reformed portion of the second area respectively.
- 2. The method of claim 1, wherein ultraviolet irradiation is used for reforming to render said perimeter and said first and said portion of the second areas hydrophilic.
- 3. The method of claim 1, wherein the water-repellent material is a resin containing a functional group that is bondable with a carboxyl group or a hydroxyl group or is a surface active agent.
- 4. The method of claim 2, wherein the water-repellent material contains a fluorine group or a silicon group.
- 5. The method of claim 2, wherein the second area surrounds the first area.
- 6. The method of claim 1, wherein the water-swelling layer contains a second reagent that is different from said reagent in the detection layer.
- 7. The method of claim 1, wherein the organic macromolecule is a thermoplastic resin, a thermosetting resin, or a rubber, or a combination thereof.
- 8. The method of claim 1, wherein the support has a multiplicity of first areas each containing a detection layer and the water-swelling layer of the second area surrounds the multiplicity of detection layers of the first areas.
- 9. The method of claim 1, wherein the divider extends upwardly from said support to a point above the surface thereof and between said two areas.
Priority Claims (1)
Number |
Date |
Country |
Kind |
8-216170 |
Jul 1996 |
JP |
|
Parent Case Info
This is a division of application Ser. No. 08/895,036, filed Jul. 16,1997.
US Referenced Citations (3)
Number |
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Date |
Kind |
5260222 |
Patel et al. |
Nov 1993 |
A |
5622870 |
Sizto et al. |
Apr 1997 |
A |
5728350 |
Kinoshita et al. |
Mar 1998 |
A |
Foreign Referenced Citations (2)
Number |
Date |
Country |
0656420 |
Jun 1995 |
EP |
WO 9619565 |
Jun 1996 |
WO |