Claims
- 1. A composition of matter containing at least one of the following sequences:
- 5'-CAT CTA CAA GCA GTC ACA-3' (SEQ ID NO:1)5'-GCA GTG CTC GCT TAG TGC-3' (SEQ ID NO:2)5'-ATT CAC TAC AAC TAC ATG-3' (SEQ ID NO:3)
- in an aqueous carrier.
- 2. A composition containing at least one of the following sequences:
- __________________________________________________________________________5'-ATC ATG TTT GAG ACC CTT CAA-3' (SEQ ID NO:4)5'-CAT CTC TTG CTC GAA GTC CA-3' (SEQ ID NO:5)5'-GAC CTG GCT GGC CCG GAC CTG ACT GAC TAC TAC-3' (SEQ ID NO:6)__________________________________________________________________________
- in an aqueous carrier.
- 3. At least one sequence chosen from among sequences of the formulas:
- __________________________________________________________________________5'-CAT CTA CAA GCA GTC ACA-3' (SEQ ID NO:1)5'-GCA GTG CTC GCT TAG TGC-3' (SEQ ID NO:2)5'-ATC CAC TAC AAC TAC ATG-3' (SEQ ID NO:3)5'-ATC ATG TTT GAG AC CTT CAA-3' (SEQ ID NO:4)5'-CAT CTC TTG CTC GAA GTC CA-3' (SEQ ID NO:5)5'-GAC CTG GCT GGC CCG GAC CTG ACT GAC TAC TAC-3'. (SEQ ID NO:6)__________________________________________________________________________
- 4. A method of determining the level of tumor suppressor gene p53 comprising the steps of:
- (1) performing reverse transcription of a sample of p53 mRNA to prepare a mix containing dNTPs and the 3' p53 oligonucleotide,
- (2) adding to the product of step (1) additional dNTPs and 5' p53 oligonucleotide,
- (3) subjecting the product obtain in step (2) to varying temperatures to provide for elongation,
- (4) applying the product of step (3) to gels,
- (5) blotting said gels obtained in step (4) onto filters,
- (6) prehybridizing the product of step (5), and
- (6) probing with a labelled internal oligonucleotide specific for a sequence of the p53 sequence and measuring the results against a .beta.-actin RT-PCR curve which has been standardized for cell number to determine the level of p53 in the sample.
Parent Case Info
This application takes priority from Provisional Application 60/014,807 filed Apr. 4, 1996.
US Referenced Citations (2)
Number |
Name |
Date |
Kind |
5726018 |
Pasternack |
Mar 1998 |
|
5811231 |
Farr et al. |
Sep 1998 |
|