Patent Grant
7122325
1. Field of the Invention
The basic unit of information transmitted from one part of the nervous system to another is a single action potential or nerve impulse. The “transmission line” for these impulses is the axon, or nerve fiber. The electrical excitability of the nerve membrane has been shown to depend on the membrane's voltage-sensitive ionic permeability system that allows it to use energy stored in ionic concentration gradients. Electrical activity of the nerve is triggered by a depolarization of the membrane, which opens channels through the membrane that are highly selective for sodium ions, which are then driven inward by the electrochemical gradient. Of the many ionic channels, the voltage-gated or voltage-sensitive sodium channel is one of the most studied. It is a transmembrane protein that is essential for the generation of action potentials in excitable cells.
The cDNAs for several Na+ channels have been cloned and sequenced. These studies have shown that the amino acid sequence of the Na+ channel has been conserved over a long evolutionary period. These studies have also revealed that the channel is a single polypeptide containing four internal repeats, or homologous domains (domains I-IV), having similar amino acid sequences. Each domain folds into six predicted transmembrane α-helices or segments: five are hydrophobic segments and one is highly charged with many lysine and arginine residues. This highly charged segment is the fourth transmembrane segment in each domain (the S4 segment) and is likely to be involved in voltage-gating. The positively charged side chains on the S4 segment are likely to be paired with the negatively charged side chains on the other five segments such that membrane depolarization could shift the position of one helix relative to the other, thereby opening the channel. Accessory subunits may modify the function of the channel.
There is a significant therapeutic utility in recombinant materials derived from the DNA of the numerous sodium channels that have been discovered. For example, the recombinant protein can be used to screen for potential therapeutics that have the ability to inhibit the sodium channel of interest. In particular, it would be useful to inhibit selectively the function of sodium channels in nerve tissues responsible for transmitting pain and pressure signals without simultaneously affecting the function of sodium channels in other tissues such as muscle, heart and brain. Such selectivity would allow for the treatment of pain without causing side effects due to cardiac, central nervous system or neuromuscular complications. Therefore, it would be useful to have DNA sequences coding for sodium channels that are selectively expressed in peripheral sensory nerve tissue. Though cDNAs from rat skeletal muscle, heart and brain are known, identification and isolation of cDNA from peripheral sensory nerve tissue, such as dorsal root ganglia, has been hampered by the difficulty of working with such tissue.
This invention relates to a cloned α-subunit of a voltage-gated tetrodotoxin-resistant sodium channel protein expressed in peripheral nerve tissue. This invention further relates to its production by recombinant technology and nucleic acid sequences encoding for this protein.
2. Summary of Related Art
An excellent review of sodium channels is presented in Catterall, TINS 16(12):500-506 (1993).
Purified Na+ channels have proven useful as therapeutic and diagnostic tools, Cherksey, U.S. Pat. No. 5,132,296.
The cDNAs for several Na+ channels have been cloned and sequenced. Numa, et al., Annals of the New York Academy of Sciences 479:338-355 (1986), describes cDNA from the electric organ of eel and two different ones from rat brain. Rogart, U.S. Pat. No. 5,380,836 describes cDNA from rat cardiac tissue. See also Rogart, Cribbs et al. Proc. Natl. Acad., Sci., 86:8170-8174 (1989). A peripheral nerve sodium channel, referred to as PN1, has been detected based on sodium current studies and hybridization to a highly conserved sodium channel probe by D'Arcangelo, et al., J. Cell Biol. 122:915-921 (1993). However, neither the DNA nor the protein were isolated and its complete nucleic acid and amino acid sequence remained unidentified. A partial amino acid sequence was presented at the 23rd Annual Meeting of the Society for Neuroscience, Nov. 7-12, 1993, Washington D.C., see Abstracts: Volume 19, Part 1: Abstract 121.7: “Nerve Growth Factor Treatment of PC12 Cells Induces the Expression of a Novel Sodium Channel Gene, Peripheral Nerve Type 1 (PN1)”, by B. L. Moss, J. Toledo-Aral and G. Mandel.
Tetrodotoxin (“TTX”), a highly potent toxin from the puffer or Fugu fish, blocks the conduction of nerve impulses along axons and in excitable membranes of nerve fibers, which leads to respiratory paralysis. TTX also binds very tightly to the Na+ channel and blocks the flow of sodium ions. The positively charged group of the toxin interacts with a negatively charged carboxylate at the mouth of the channel on the extracellular side of the membrane, thus obstructing the conductance pathway.
Studies using TTX as a probe have shed much light on the mechanism and structure of Na+ channels. There are three Na+ channel subtypes, defined by the affinity for TTX, which can be measured by the IC50 values: TTX-sensitive Na+ channels (IC50≈1 nM), TTX-insensitive Na+ channels (IC50≈1-5 μM), and TTX-resistant Na+ channels (IC50≧100 μM)
TTX-insensitive action potentials were first studied in rat skeletal muscle. Redfern, et al., Acta Physiol. Scand. 82:70-78 (1971). Subsequently, these action potentials were described in other mammalian tissues, including newborn mammalian skeletal muscle, mammalian cardiac muscle, mouse dorsal root ganglion cells in vitro and in culture, cultured mammalian skeletal muscle and L6 cells. Rogart, Ann. Rev. Physiol. 43:711-725 (1980).
Dorsal root ganglia neurons possess both TTX-sensitive (IC50≅0.3 nM) and TTX-resistant (IC50≅100 μM) sodium channel currents, as described in Roy, et al., J. Neurosci. 12:2104-2111 (1992).
TTX-resistant sodium currents have also been measured in rat nodose and petrosal ganglia, Ikeda, et al., J. Neurophysiol. 55:527-539 (1986) and Stea, et al., Neurosci. 47:727-736 (1992).
One aspect of the present invention is a purified and isolated DNA sequence encoding for a mammalian peripheral nerve sodium channel protein, in particular, the α-subunit of this protein. A preferred embodiment of the invention is a purified and isolated DNA sequence encoding a mammalian peripheral nerve TTX-resistant sodium channel.
Further aspects of the invention include expression vectors comprising the DNA of the invention, host cells transformed or transfected by these vectors, specifically mammalian cells, and a cDNA library of these host cells.
Another aspect of the present invention is a recombinant polynucleotide comprising a nucleic acid sequence derived from the DNA sequence of this invention.
Still another aspect of the invention is the rat and human peripheral nerve TTX-resistant sodium channel protein encoded by the DNA of this invention.
Also forming part of this invention is an assay for inhibitors of the peripheral nerve TTX-resistant sodium channel protein comprising contacting a compound suspected of being an inhibitor with expressed sodium channel and measuring the activity of the sodium channel.
Further provided is a method of inhibiting the activity of the peripheral nerve TTX-resistant sodium channel comprising administering an effective amount of a compound having an IC50 of 10 μM or less.
).
The present invention relates to sodium channel proteins present in peripheral nerve tissue. Specific embodiments include such sodium channels that are TTX-resistant and are expressed exclusively in sensory neurons. Degenerate oligonucleotide-primed RT-PCR analysis was performed on RNA from the rat central and peripheral nervous systems, in particular from rat dorsal root ganglia (“DRG”). The α-subunit of a voltage-gated, TTX-resistant sodium channel from rat DRG has been cloned and functionally expressed in Xenopus oocytes.
In particular, the present invention relates to a purified and isolated DNA sequence encoding for a rat peripheral nerve TTX-resistant sodium channel. The term “purified and isolated DNA” refers to DNA that is essentially free, i.e. contains less than about 30%, preferably less than about 10%, and even more preferably less than about 1% of the DNA with which the DNA of interest is naturally associated. Techniques for assessing purity are well known to the art and include, for example, restriction mapping, agarose gel electrophoresis, and CsCl gradient centrifugation. The term “DNA” is meant to include cDNA made by reverse transcription of mRNA or by chemical synthesis.
Specifically, the invention relates to DNA having the nucleotide sequence set forth in
The present invention also relates to a purified and isolated DNA sequence encoding a human peripheral nerve TTX-resistant sodium channel. Specifically, the invention includes DNA having the nucleotide sequence set forth in
The 1956-amino acid protein encoded by the cDNA of the invention is designated herein as peripheral nerve sodium channel type 3 (“PN3”). The DNA sequence in
The term “cDNA” or complementary DNA refers to single-stranded or double-stranded DNA sequences obtained by reverse transcription of mRNA isolated from a donor cell. For example, treatment of mRNA with a reverse transcriptase such as AMV reverse transcriptase or M-MuLV reverse transcriptase in the presence of an oligonucleotide primer will furnish an RNA-DNA duplex which can be treated with RNase H, DNA polymerase, and DNA ligase to generate double-stranded cDNA. If desired, the double-stranded cDNA can be denatured by conventional techniques such as heating to generate single-stranded cDNA. The term “cDNA” includes cDNA that is a complementary copy of the naturally occurring mRNA as well as complementary copies of variants of the naturally occurring mRNA, that have the same biological activity. Variants would include, for example, insertions, deletions, sequences with degenerate codons and alleles. Anexample of an insertion is a single additional Gln codon between the Pro584 and Ala585 codons of the full-length cDNA sequence of PN3, found in several clones.
The term “cRNA” refers to RNA that is a copy of the mRNA transcribed by a cell. CRNA corresponding to mRNA transcribed from a DNA sequence encoding the α-subunit of a mammalian peripheral nerve TTX resistant sodium channel protein is contemplated by this invention.
As mentioned above, it is believed that homologs of the rat TTX-resistant sodium channel described herein are also expressed in other mammalian peripheral nerve tissue, in particular, human tissue. The rat sodium channel cDNA of the present invention can be used as a probe to discover whether a voltage-gated TTX-resistant sodium channel exists in human peripheral nerve tissue and, if it does, to aid in isolating the cDNA for the human protein.
The human homologue of the rat TTX-resistant PN3 can be cloned using a human DRG cDNA library. Human DRG are obtained at autopsy. The frozen tissue is homogenized and the RNA extracted with guanidine isothiocyanate (Chirgwin, et al. Biochemistry 18:5294-5299, 1979). The RNA is size-fractionated on a sucrose gradient to enrich for large mRNAs because the sodium channel α-subunits are encoded by large (7-11 kb) transcripts. Double-stranded cDNA is prepared using the Superscript Choice cDNA kit (GIBCO BRL) with either oligo(dT) or random hexamer primers. EcoRI adapters are ligated onto the double-stranded cDNA which is then phosphorylated. The cDNA library is constructed by ligating the double-stranded cDNA into the bacteriophage-lambda ZAP II vector (Stratagene) followed by packaging into phage particles.
Phage are plated out on 150 mm plates on a lawn of XLI-Blue MRF1 bacteria (Stratagene) and plaque replicas are made on Hybond N nylon membranes (Amersham). Filters are hybridized to a rat PN3 cDNA or CRNA probe by standard procedures and detected by autoradiography or chemiluminescence. The signal produced by the rat PN3 probe hybridizing to positive human clones at high stringency should be stronger than obtained with rat brain sodium channel probes hybridizing to these clones. Positive plaques are further purified by limiting dilution and rescreened by hybridization or PCR. Restriction mapping and polymerase chain reaction will identify overlapping clones that can be assembled by standard techniques into the full-length human homologue of rat PN3. The human clone can be expressed by injecting CRNA transcribed in vitro from the full-length cDNA clone into Xenopus oocytes, or by transfecting a mammalian cell line with a vector containing the cDNA linked to a suitable promoter.
The human homologue of the rat TTX-resistant PN3 was cloned using the procedure outlined above. From human DRG, RNA was extracted and used to prepare cDNA and the cDNA library. The human PN3 was then obtained using a PN3 probe, and expressed as described above. A comparison of the human PN3 sequence (SEQ ID NO: 10) to other known human and rat voltage-gated sodium channels revealed that the greatest homology is with the rat PN3 channel, where the corresponding human gene is 83% homologous to the rat sequence. The most closely related human channel is the heart I channel, having 64% identity for the amino acid sequence. A similar relationship was observed for rat PN3 in that the most closely related channel was the rat heart channel. A variant of rat PN3 was detected in which an extra Gln residue was present in the interdomain I/II loop, however, no such variant was found in the human DRG. The PN3 and SNS rat DRG sodium channels are very closely related and differ by only seven residues. Six of these seven residues are identical in the human PN3 and rat PN3, so that the human PN3 is more similar to the rat PN3 than to the SNS channel.
Analysis of the open reading frame revealed that the human PN3 sequence has all the hallmark structural features of sodium channels that are predicted from the amino acid sequence: 24 transmembrane segments, charged residues in the S4 transmembrane segments, and the IFM sequence within the highly conserved interdomain II-IV region which constitutes the fast inactivation gate. In addition the human PN3 channel had the identical sequence as rat PN3 for the TTX-sensitivity site within the domain I S5-S6 loop, where there is a Ser in position 357 in human PN3 and position 356 in rat PN3, rather than a Cys residue which is present in all other, that is non-PN3 type, TTX-insensitive/resistant channels. The human and rat channels also shared N-glycosylation consensus sites and cAMP-dependent kinase sites which included several unusual sites in domain II and interdomain II-III.
The present invention also includes expression vectors comprising the DNA or the cDNA described above, host cells transformed with these expression vectors capable of producing the sodium channel of the invention, and cDNA libraries comprising such host cells.
The term “expression vector” refers to any genetic element, e.g., a plasmid, a chromosome, a virus, behaving either as an autonomous unit of polynucleotide expression within a cell or being rendered capable of replication by insertion into a host cell chromosome, having attached to it another polynucleotide segment, so as to bring about the replication and/or expression of the attached segment. Suitable vectors include, but are not limited to, plasmids, bacteriophages and cosmids. Vectors will contain polynucleotide sequences which are necessary to effect ligation or insertion of the vector into a desired host cell and to effect the expression of the attached segment. Such sequences differ depending on the host organism, and will include promoter sequences to effect transcription, enhancer sequences to increase transcription, ribosomal binding site sequences and transcription and translation termination sequences.
The term “host cell” generally refers to prokaryotic or eukaryotic organisms and includes any transformable or transfectable organism which is capable of expressing a protein and can be, or has been, used as a recipient for expression vectors or other transferred DNA. Host cells can also be made to express protein by direct injection with exogenous CRNA translatable into the protein of interest. A preferred host cell is the Xenopus oocyte.
The term “transformed” refers to any known method for the insertion of foreign DNA or RNA sequences into a host prokaryotic cell. The term “transfected” refers to any known method for the insertion of foreign DNA or RNA sequences into a host eukaryotic cell. Such transformed or transfected cells include stably transformed or transfected cells in which the inserted DNA is rendered capable of replication in the host cell. They also include transiently expressing cells which express the inserted DNA or RNA for limited periods of time. The transformation or transfection procedure depends on the host cell being transformed. It can include packaging the polynucleotide in a virus as well as direct uptake of the polynucleotide, such as, for example, lipofection or microinjection. Transformation and transfection can result in incorporation of the inserted DNA into the genome of the host cell or the maintenance of the inserted DNA within the host cell in plasmid form. Methods of transformation are well known in the art and include, but are not limited to, viral infection, electroporation, lipofection and calcium phosphate mediated direct uptake.
It is to be understood that this invention is intended to include other forms of expression vectors, host cells and transformation techniques which serve equivalent functions and which become known to the art hereto.
The term “cDNA library” refers to a collection of clones, usually in a bacteriophage, or less commonly in bacterial plasmids, containing cDNA copies of mRNA sequences derived from a donor cell or tissue.
In addition, the present invention contemplates recombinant polynucleotides, of about 15 to 20 kb, preferably 10 to 15 kb nucleotides in length, comprising a nucleic acid sequence segment of the DNA of SEQ ID NOs: 1 and 9. The invention also includes a recombinant polynucleotide comprising a nucleic acid subsequence derived from the DNA of SEQ ID NOs: 1 and 9. The term “subsequence” refers to a nucleic acid sequence having substantially the same DNA as the sequence of the invention, having certain nucleotide additions or deletions.
The term “polynucleotide” as used herein refers to a polymeric form of nucleotides of any length, either ribonucleotides or deoxyribonucleotides. This term refers only to the primary structure of the molecule. Thus, this term includes double- and single-stranded DNA, as well as double- and single-stranded RNA. It also includes modified, for example, by methylation and/or by capping, and unmodified forms of the polynucleotide. The term “derived from” a designated sequence, refers to a nucleic acid sequence that is comprised of a sequence of approximately at least 6-8 nucleotides, more preferably at least 10-12 nucleotides, and, even more preferably, at least 15-20 nucleotides that correspond to, i.e., are homologous or complementary to, a region of the designated sequence. The derived sequence is not necessarily physically derived from the nucleotide sequence shown, but may be derived in any manner, including for example, chemical synthesis or DNA replication or reverse transcription, which are based on the information provided by the sequences of bases in the region(s) from which the polynucleotide is derived. Further, the term “polynucleotide” is intended to include a recombinant polynucleotide, which is of genomic, cDNA, semisynthetic or synthetic origin which, by virtue of its origin or manipulation is not associated with all or a portion of the polynucleotide with which it is associated in nature and/or is linked to a polynucleotide other than that to which it is linked in nature.
The polynucleotides of the invention can be bound to a reporter molecule to form a polynucleotide probe useful for Northern and Southern blot analysis and in situ hybridization.
The term “reporter molecule” refers to a chemical entity capable of being detected by a suitable detection means, including, but not limited to, spectrophotometric, chemiluminescent, immunochemical, or radiochemical means. The polynucleotides of this invention can be conjugated to a reporter molecule by techniques well known in the art. Typically the reporter molecule contains a functional group suitable for attachment to or incorporation into the polynucleotide. The functional groups suitable for attaching the reporter group are usually activated esters or alkylating agents. Details of techniques for attaching reporter groups are well known in the art. See, for example, Matthews, J. A., Batki, A., Hynds, C., and Kricka, L. J., Anal. Biochem., 151:205-209 (1985) and Engelhardt et al., European Patent Application No. 0 302 175.
The invention not only includes the entire protein expressed by the cDNA sequence of
The polypeptides of the invention are highly useful for the development of antibodies against PN3. Such antibodies can be used in affinity chromatography to purify recombinant sodium channel proteins or polypeptides, or they can be used as a research tool. For example, antibodies bound to a reporter molecule can be used in histochemical staining techniques to identify other tissues and cell types where PN3 is present, or they can be used to identify epitopic or functional regions of the sodium channel protein of the invention.
The antibodies can be monoclonal or polyclonal and can be prepared by techniques that are well known in the art. Polyclonal antibodies are prepared as follows: an immunogenic conjugate comprising PN3 or a fragment thereof, optionally linked to a carrier protein, is used to immunize a selected mammal such as a mouse, rabbit, goat, etc. Serum from the immunized mammal is collected and treated according to known procedures to separate the immunoglobulin fraction. Monoclonal antibodies are prepared by standard hybridoma cell technology based on that reported by Kohler and Milstein in Nature 256:495-497 (1975): spleen cells are obtained from a host animal immunized with the PN3 protein or a fragment thereof, optionally linked to a carrier. Hybrid cells are formed by fusing these spleen cells with an appropriate myeloma cell line and cultured. The antibodies produced by the hybrid cells are screened for their ability to bind to expressed PN3 protein. A number of screening techniques well known in the art, such as, for example, forward or reverse enzyme-linked immunosorbent assay screening methods may be employed. The hybrid cells producing such antibodies are then subjected to recloning and high dilution conditions in order to select a hybrid cell that secretes a homogeneous population of antibodies specific to the PN3 protein. In addition, antibodies can be raised by cloning and expressing nucleotide sequences or mutagenized versions thereof coding at least for the amino acid sequences required for specific binding of natural antibodies, and these expressed proteins used as the immunogen. Antibodies may include the complete immunoglobulin or a fragment thereof. Antibodies may be linked to a reporter group such as is described above with reference to polynucleotides.
As mentioned above, the invention pertains to the cloning and functional expression, in Xenopus oocytes, of a rat peripheral nerve TTX-resistant sodium channel. Specifically, the α-subunit of the sodium channel was cloned and expressed. Accordingly, the invention encompasses a rat peripheral nerve TTX-resistant sodium channel α-subunit encoded by the cDNA set forth in
The sodium channel comprises an α- and a β-subunit. The β-subunit may modulate the function of the channel. However, since the α-subunit is all that is required for the channel to be fully functional, the expression of the cDNA in
Functional expression in Xenopus oocytes shows that PN3 is a voltage-gated sodium channel with a depolarized activation potential, slow inactivation kinetics, and resistant to a high concentration of TTX. PN3 may correspond to the sodium channel mediating TTX-resistant currents in small neurons of the DRG, that have been described in the literature. See for example, Kostyuk, et al., Neurosci. 6:2423-2430 (1981); McLean, et al., Molec. Cell. Biochem. 80:95-107 (1988); Roy, et al., supra; Caffrey, et al., Brain Res. 592:283-297 (1992); Elliott, et al., J. Physiol. 463:39-56 (1993); and Ogata, et al., J. Physiol. 466:9-37 (1993).
Northern blot analysis indicates that PN3 is encoded by an ˜7.5 kb transcript and nucleotide sequence analysis of the PN3 cDNA identifies a 5868-base open reading frame, shown in FIG. 1. The deduced amino acid sequence of PN3, shown in
The present invention also includes the use of the voltage-gated, TTX-resistant sodium channel α-subunit as a therapeutic target for compounds to treat disorders of the peripheral nervous system including, but not limited to, allodynia, hyperalgesia, diabetic neuropathy, traumatic injury and AIDS-associated neuropathy. The invention allows for the manipulation of genetic materials by recombinant technology to produce polypeptides that possess the structural and functional characteristics of the voltage-gated, TTX-resistant sodium channel α-subunit found in peripheral nerve tissue, particularly in sensory nerves. Site directed mutagenesis can be used to provide such recombinant polypeptides. For example, synthetic oligonucleotides can be specifically inserted or substituted into the portion of the gene of interest to produce genes encoding for and expressing a specific mutant. Random degenerate oligonucleotides can also be inserted and phage display techniques can be used to identify and isolate polypeptides possessing a functional property of interest.
Sodium channels in peripheral nerve tissue play a large role in the transmission of nerve impulses, and therefore are instrumental in understanding neuropathic pain transmission. Neuropathic pain falls into two categories: allodynia, where a normally non-painful stimulus becomes painful, and hyperalgesia, where a usually normal painful stimulus becomes extremely painful. The ability to inhibit the activity of these sodium channels, i.e., reduce the conduction of nerve impulses, will affect the nerve's ability to transmit pain. Selective inhibition of sodium channnels in sensory neurons such as dorsal root ganglia will allow the blockage of pain impulses without complicating side effects caused by inhibition of sodium channels in other tissues such as brain and heart. In addition, certain diseases are caused by sodium channels that produce impulses at an extremely high frequency. The ability to reduce the activity of the channel can then eliminate or alleviate the disease. Accordingly, potential therapeutic compounds can be screened by methods well known in the art, to discover whether they can inhibit the activity of the recombinant sodium channel of the invention. Barram, M., et al., Naun-Schmiedeberg's archives of Pharmacology, 347: 125-132 (1993) and McNeal, E. T. et al., J. Med. Chem., 28: 381-388 (1985). For similar studies with the acetyl choline receptor, see, Claudio et al., Science, 238: 1688-1694 (1987).
The sodium channel of the present invention has the most restrictive tissue distribution of the channels that have been studied. This is of significant value to develop therapeutics that will have a specific target, i.e., that will not inhibit a particular channel in a wide range of tissues. Seven main tissue types were screened by RT-PCR for expression of the unique 410 base amplicon corresponding to positions 5893-6302 of SEQ ID NO:1. PN3 was present in three of the tissues studied: DRG, nodose ganglia and sciatic nerve tissue. PN3 was not present in the remaining tissues studied: brain, spinal cord, heart or skeletal muscle tissue. In view of the previous detection of a sodium channel PN1 mRNA in the peripheral nervous system, (D'Arcangelo et al) other tissues were screened by RT-PCR for the presence of PN1. PN1 was detected in brain, heart, spinal cord and superior cervical ganglia, under conditions in which PN3 was not detected. A tissue distribution profile of human PN3 was determined by analysis of RNA from selected human tissues and commerically available cDNA libraries by RT-PCR. hPN3 was present in two of the tissues studied: DRG and sciatic nerve tissue. hPN3 was not present in the remaining tissues studied: brain, spinal cord, heart, or skeletal muscle tissue.
This invention is directed to inhibiting the activity of PN3 in DRG, nodose ganglia and sciatic nerve tissues. However, it is to be understood that further studies may reveal that PN3 is present in other tissues, and as such, those tissues can also be targeted areas. For example, the detection of PN3 mRNA in nodose ganglia suggests that PN3 may conduct TTX-resistant sodium currents in this and other sensory ganglia of the peripheral nervous system. In addition, it has been found that proteins not normally expressed in certain tissues, are expressed in a disease state. Therefore, this invention is intended to encompass the inhibition of PN3 in tissues and cell types where the protein is normally expressed, and in those tissues and cell types where the protein is only expressed during a disease state.
Another significant characteristic of PN3 is that it is TTX-resistant. It is believed that TTX-resistant sodium channels play a key role in transmitting nerve impulses relating to sensory inputs such as pain and pressure. This will also facilitate the design of therapeutics that can be targeted to a specific area such as the peripheral nerve tissue. Studies of the TTX-resistant site on the protein will facilitate the development of a selective inhibitor. This site is shown in
The invention also pertains to an assay for inhibitors of peripheral nerve TTX-resistant sodium channel protein comprising contacting a compound suspected of being an inhibitor with expressed sodium channel and measuring the activity of the sodium channel. The compound can be a substantially pure compound of synthetic origin combined in an aqueous medium, or the compound can be a naturally occurring material such that the assay medium is an extract of biological origin, such as, for example, a plant, animal, or microbial cell extract. PN3 activity can be measured by methods such as electrophysiology (two electrode voltage clamp or single electrode whole cell patch clamp), guanidinium ion flux assays and toxin-binding assays. An “inhibitor” is defined as generally that amount that results in greater than 50% decrease in PN3 activity, preferably greater than 70% decrease in PN3 activity, more preferably, greater than 90% decrease in PN3 activity.
In addition, the present invention encompasses a method of alleviating pain by inhibiting the activity of the peripheral nerve TTX-resistant sodium channel comprising administering a therapeutically effective amount of a compound having an IC50 of 10 μM or less, preferably ≦1 μM. Potential therapeutic compounds are identified based on their ability to inhibit the activity of PN3. Therefore, the aforementioned assay can be used to identify compounds having a therapeutically effective IC50.
The term “IC50” refers to the concentration of a compound that is required to inhibit by 50% the activity of expressed PN3 when activity is measured by electrophysiology, flux assays and toxin-binding, assays, as mentioned above.
The basic molecular biology techniques employed in accomplishing features of this invention, such as RNA and DNA and plasmid isolation, restriction enzyme digestion, preparation and probing of a cDNA library, sequencing clones, constructing expression vectors, transforming cells, maintaining and growing cell cultures and other general techniques are well known in the art, and descriptions of such techniques can be found in general laboratory manuals such as Molecular Cloning: A Laboratory Manual by Sambrook, et al. (Cold Spring Harbor Laboratory Press, 2nd edition, 1989). Accordingly, the following examples are merely illustrative of the techniques by which the invention can be practiced.
Materials
The plasmid, pBK-CMV, was obtained from Stratagene (La Jolla, Calif.); plasmid, pBSTA, was obtained from A. Goldin at the University of California, Irvine and is described by Goldin, et al., in Methods in Enzymology (Rudy & Iverson, eds) 207:279-297.
Lumbar DRG #4 and #5 (L4 and L5), brain and spinal cord were removed from anesthetized adult male Sprague-Dawley rats under a dissecting microscope. The tissues were frozen in dry ice and homogenized with a Polytron homogenizer; the RNA was extracted by the guanidine isothiocyanate procedure (Chomczynksi, et al., Anal. Biochemistry 162:156-159, 1987). Total RNA (5 μg of each sample) was dissolved in MEN buffer containing 50% formamide, 6.6% formaldehyde and denatured at 65° C. for 5-10 min. The RNA was electrophoresed through a 0.8% agarose gel containing 8.3% formaldehyde in MEN buffer. The electrode buffer was MEN buffer containing 3.7% formaldehyde; the gel was run at 50 V for 12-18 hr.
After electrophoresis, the gel was rinsed in 2×SSC and the RNA was transferred to a Duralose membrane (Stratagene) with 2O×SSC by capillary action; the membrane was baked under vacuum at 80° C. for 1 hr. The membrane was prehybridized in 50% formamide, 5×SSC, 50 mM sodium phosphate, pH 7.1, 1×Denhardt's solution, 0.5% SDS, and sheared, heat-denatured salmon sperm DNA (1 mg/ml) for 16 hr at 42° C. The membrane was hybridized in 50% formamide, 5×SSC, 50 mM sodium phosphate, pH 7.1, 1×Denhardt's solution, 0.5% SDS, and sheared, heat-denatured salmon sperm DNA (200 μg/ml) with a 32P-labeled cRNA probe (ca. 1-3×106 cpm/ml) that was complementary to nucleotides 4637-5868 of the rat bran IIA sodium channel μ-subunit sequence for 18 hr at 42° C.; the cRNA probe was synthesized in vitro with T7 RNA polymerase (Pharmacia) using pEAF8 template DNA, Noda et al., Nature, 320:188-192 (1986), (obtained from W. A. Canterall, University of Washington) that had been linearized with BstEII.
The membrane was rinsed with 2×SSC, 0.1% SDS at room temperature for 20 min and then washed sequentially with: 2×SSC, 0.1% SDS at 55° C. for 30 min, 0.2×SSC, 0.1% SDS at 65° C. for 30 min, 0.2×SSC, 0.1% SDS at 70° C. for 30 min, and 0.2×SSC, 0.1% SDS, 0.1% sodium pyrophosphate at 70° C. for 20 min. The filter was exposed against Kodak X-omat AR film at −80° C. with intensifying screens for up to 2 weeks.
Size markers, including ribosomal 18S and 28S RNAs and RNA markers (GIBCO BRL), were run in parallel lanes of the gel. Their positions were determined by staining the excised lane with ethidium bromide (0.5 μg/ml) followed by photography under UV light. The pEAF8 probe hybridized to mRNAs in the DRG sample with sizes of 11 kb, 9.5 kb, 7.3 kb, and 6.5 kb, estimated on the basis of their positions relative to the standards. When the membrane was reprobed with a cloned fragment corresponding to the novel sodium channel domain IV (SEQ ID NO:3), the 7.3 kb transcript is detected in the DRG mRNA, but this size mRNA is not detected in brain or spinal cord. The probe's sequence (SEQ ID NO:3) was as follows:
The probe was obtained as follows: RT-PCR was performed on RNA isolated from rat DRG using degenerate ologonucleotide primers that were designed based on the homologies between known sodium channels in domain IV. The domain IV products were cloned in to a plasmid vector, transformed into E. coli and single colonies isolated. The domain IV specific PCR products obtained from several of these colonies were individually sequenced. Cloned novel domain IV sequence (SEQ ID NO:3) was labelled with 32P by random priming and used to probe a Northern blot of rat brain, spinal cord and DRG RNA.
Nucleotides 16-689 of the probe's sequence corresponds to nucleotides 4502-5175 of
This result suggests that the 7.3 kb mRNA encoding PN3 is uniquely expressed in peripheral neurons and that SEQ ID NO:3 can be used to detect/isolate/differentiate peripheral nervous system sodium channels from others.
EcoRI-adapted cDNA was prepared from normal adult male Sprague-Dawley rat DRG poly(A)+ RNA using the SuperScript Choice System (GIBCO BRL). cDNA (>4 kb) was selected by sucrose gradient fractionation as described by Kieffer, Gene 109:115-119 (1991). The cDNA was then ligated into the Zap Express vector (Stratagene), and packaged with the Gigapack II XL lambda packaging extract (Stratagene). Phage (3.5×105) were screened by filter hybridization with a 32P-labelled probe (rBIIa, bases 4637-5868 of Auld, et al., Neuron 1:449-461 (1988)). Filters were hybridized in 50% formamide, 5×SSPE, 5×Denhardt's solution, 0.5% SDS, 250 μg/ml sheared, denatured salmon sperm DNA, and 50 mM sodium phosphate at 42° C. and washed in 0.5×SSC/0.1%. SDS at 50° C. Positive clones were excised in vivo into pBK-CMV using the ExAssist/XLOLR system (Stratagene). Southern blots of EcoRI-digested plasmids were hybridized with the 32P-labelled DNA probe, (SEQ ID NO: 3), representing a novel domain IV segment amplified from DRG RNA with degenerate oligonucleotide primers.
Southern filters were hybridized in 50% formamide, 6×SSC, 5×Denhardt's solution, 0.5% SDS, and 100 μg/ml sheared, denatured salmon sperm DNA at 42° C. and were washed in 0.1×SSC/0.1% SDS at 65° C.
A plasmid containing a full-length cDNA was identified, designated peripheral nerve sodium channel type 3 (“PN3”), and sequenced on both strands. For oocyte expression analysis, the PN3 cDNA was excised from the vector and, after blunting the ends subcloned into pBSTA.
The deduced amino acid sequence (SEQ ID NO:2) of PN3 is shown in FIG. 2. PN3 contains four homologous domains, represented as the regions marked I-IV. Each domain consists of six putative α-helical transmembrane segments, represented as S1-S6. The positively charged residues in the voltage sensor (S4 segments) and the inactivation gate between IIIS6 and IVS1 are highly conserved in PN3. Sites for cAMP-dependent phosphorylation and N-linked glycosylation shown experimentally to exist in other sodium channels (See Catterall, Physiol. Rev. 72:S15-S48 (1992)) are also present in PN3. This is shown in
Also identified were several PN3 partial clones which contained a single additional Gln between Pro584 and Ala585 () of the full-length PN3 sequence. The corresponding RNA had three additional nucleotides, thus establishing that the extra amino acid was not a cloning artifact.
Similar procedures have furnished partial clones coding for additional sodium channel proteins in dorsal root ganglia. Sequencing data of these clones revealed that one of these other clones had a sequence which encoded the disclosed partial amino acid sequence of the sodium channel protein, PN1.
Sequence analyses were done to compare the amino acid sequence of PN3 and selected cloned rat sodium channels, using the Gap, PileUp, and Distances programs of the Wisconsin Sequence Analysis Package (Genetics Computer Group, Inc.). The sodium channels evaluated were as follows:
where rBI and rBII are rat brain sodium channels I and II, respectively, described in Noda, et al., Nature 320:188-192 (1986); rBIII is rat brain sodium channel III, described in Joho, et al., Molec. Brain Res. 7:105-113 (1990); rSkMl is rat skeletal muscle, described in Trirmer, et al., Neuron 3:33-49 (1989); and rHl is rat heart sodium channel, described in Rogart, et al., PNAS 86:8170-8174 (1989). The sequence homology between PN3 and the TTX-insensitive cardiac channel and their slow kinetics suggest that they belong to a unique subfamily of sodium channels.
Brain, spinal cord, DRG, nodose ganglia, superior cervical ganglia, sciatic nerve, heart and skeletal muscle tissues were isolated from anesthetized, normal adult male Sprague-Dawley rats and were stored at −80° C. RNA was isolated from each tissue using RNAzol (Tel-Test, Inc.). Random-primed cDNA was reverse transcribed from 500 ng of RNA from each tissue. PCR primers corresponding to positions 5893-5912 of
5′ AAG GCA CTC AGG CAT GCA CA 3′ (SEQ ID NO:4) and antisense corresponding to positions 6282-6302 of
5′ TGG CCG ACT CAC AGG TAT TG 3′ (SEQ ID NO:5) targeted the 3′-untranslated region of PN3 and defined a 410 bp amplicon (SEQ ID NO:6) corresponding to positions 5893-6302 of FIG. 1:
Thermal cyclek parameters: 30 s/94° C., 30 s/57° C., 1 min/72° C. (24 cycles); 30 s/94° C., 30 s/57° C., 5 min/72° C. (1 cycle). A positive control (1 ng pBK-CMV/PN3) and a no-template control were also included. cDNA from each tissue was also PCR amplified using primers specific for glyceraldehyde-3-phosphate dehydrogenase to demonstrate template viability, as described by Tso, et al., Nucleic Acid Res. 13:2485-2502 (1985). PN3 PCR amplicons from nodose ganglia and sciatic nerve were confirmed by nucleotide sequence analysis.
Tissue distribution profile of PN3 by analysis of RNA from selected rat tissues by RT-PCR was as follows:
As can be seen from Table 2, RNA analysis suggests that PN3 mRNA expression is limited to DRG and nodose ganglia of the PNS. PN3 mRNA was readily detected in DRG and nodose ganglia by amplification for only 25 cycles; a small amount of PN3 mRNA was also detected in sciatic nerve after 25 cycles. PN3 mRNA was not detected in brain, spinal cord, heart, skeletal muscle, or superior cervical ganglia after 35 cycles of amplification.
Additional RT-PCR analyses of DRG mRNA detected rBI, RBII, RBIII, and rHl, along with peripheral nerve sodium channel type 1 (PN 1), described in D'Arcangelo, et al., supra. PN1 was also detected in brain, heart, spinal cord and superior cervical ganglia under conditions in which PN3 was not detected.
Oligonucleotide probe sequences were identified from the unique 3′-untranslated region of PN3 (sense and antisense probes were complementary to each other). The sense probe had the following sequence:
5′ AGG CAC TCA GGC ATG CAC AGG GCA GGT TCC AAT GTC TTT CTC
TGC T 3′ (SEQ ID NO:7) and the antisense had the following sequence:
3′ TCC GTG AGT CCG TAC GTG TCC CGT CCA AGG TTA CAG AAA GAG
ACG A 5′ (SEQ ID NO:8) both corresponding to positions 5894-5939.
Normal rats were perfused with 4% paraformaldehyde; lumbar DRG #4-#6 (L4-L6) were removed, postfixed in the same solution, and cryoprotected in 20% sucrose. Frozen sections (10 μm) were cut and hybridized overnight at 39° C. in a solution containing 35S-ATP labelled oligonucleotides (specific activity=5×107−1×108 cpm/μg), 50% formamide, 4×SSC, 0.5 mg/ml salmon sperm DNA, and 1×Denhardt's solution. Sections were washed over a period of 6 hours in 2×−0.2×SSC containing 0.1%-β-mercaptoethanol, dehydrated in a series of ethanols (50%-100%) containing 0.3 M ammonium acetate, and apposed to sheet film (Amersham Bmax) or emulsion (Amersham LM-1) for 2 and 5 weeks, respectively. The cell surface area of all neurons with a distinct nucleus was measured from stained sections obtained from 3 ganglia using a computerized image analysis system (Imaging Research, Inc.).
In situ hybridization of these PN3-specific oligonucleotide probes to rat DRG showed that PN3 mRNA is specifically expressed in neuronal cells. The labelled cells were distributed throughout the ganglia, but most labelled neurons were small in somal area (<1500 μm2). PN3 mRNA was not detected in the axons of L4 and L5 DRG neurons by in situ analysis; however, RT-PCR analysis detected PN3 mRNA in the sciatic nerve. This difference is attributed to the greater sensitivity of RT-PCR amplification versus in situ hybridization.
cRNA was prepared from PN3 subcloned into pBSTA using a T7 in vitro transcription kit (Ambion, mMessage mMachine) and was injected into stage V and VI Xenopus oocytes using a Nanojector (Drummond), as described in Goldin, supra. After 2.5 days at 20° C., the oocytes were impaled with agarose-cushion electrodes (0.3-0.8 MOhm) and voltage-clamped with a Geneclamp 500 amplifier (Axon Instruments) in TEV mode. See Schreibmayer, et al., Pflugers Arch. 426:453-458 (1994).
Stimulation and recording were controlled by a computer running pClamp (Axon Instruments), Kegel, et al. J. Neurosci. Meth. 12:317-330 (1982). Oocytes were perfused with a solution containing: 81 mM NaCl, 2 mM KC1, 1 mM MgCl2, 0.3 mM CaCl2, 20 mM Hepes-NaOH, pH 7.5.
Examination of the current/voltage relationship reveals a strikingly depolarized channel activation potential, as can be seen in
Sodium channels are distinctively sensitive or insensitive to neurotoxins such as TTX. The TTX-sensitive brain and skeletal muscle sodium channels are blocked by nanomolar TTX concentrations, whereas the TTX-insensitive cardiac sodium channels are blocked by micromolar TTX concentrations. In rat heart sodium channel 1 (RHl), Cys374 is a critical determinant of TTX-insensitivity, as shown in Satin, et al., Science 256:1202-1205(1992); in the TTX-sensitive rBI, RBII, RBIII, and rSkMl, the corresponding residue is either Phe or Tyr. In PN3, this position is occupied by a Ser residue (Ser356). When expressed in Xenopus oocytes, the PN3 sodium current is highly resistant to TTX (IC50≧100 μM).
A 15-mer peptide(CDPNLPNSNGSRGNC) was synthesized and and coupled to keyhole limpet hemocyanin prior to injection into rabbit. The sequence of the peptide corresponded to residues 1679-1693 of FIG. 2. Out of the two rabbits injected with the antigen only one yielded antiserum that is useful in characterizing the PN3 ion channel protein. The antiserum was then affinity purified by passage through a peptide affinity column. Immunohistochemical experiments substantiated earlier observations using PN3 antisense oligonucleotide probe(in situ hybridization) that PN3 was largely localized in the small sensory neurons of the dorsal root ganglia (DRG). In addition to the sensory neurons of DRG, a small number of transmission neurons in lamina 10 of the spinal cord showed immunoreactivity with the PN3 antibody. Because only a subset of neurons were positive for PN3 expression, PN3 mRNA could have been undetectable by RT-PCR assays using the entire spinal cord (dilution effect).
Immunoprecipitation experiments indicated that PN3 expressing Chinese hamster lung (CHL) cells had a ˜250 kD protein which corresponds to the α-subunit. Since the peptide sequence does not match with any other protein, particularly other sodium channels, the antibody could be very specific reagent to characterize the PN3 protein. In addition, the antibody could be used as a tool to understand the role of PN3 in nociceptive pathways. By infusing the antibody in rats so that it ‘soaks-up’ all available PN3 protein and testing the rats in pain models one could begin to investigate the role of PN3 function in pain pathways.
A variant of PN3, PN3a has been identified by sequencing a full-length cDNA clone. The sequence of PN3a is identical to that of PN3, including the 5′- and 3′-UTR, except for an additional amino acid, Gln, at Pro584 of PN3 set forth in SEQ ID NO: 2. The insertion of Gln in this region was previously reported from RT-PCR experiments. PN3a expressed at a higher level than PN3 in Xenopus oocytes and exhibited otherwise the same characteristics as PN3, such as resistance to high concentrations of TTX and depolarized activation potential.
Another cDNA clone had the same sequence as PN3 in the coding region, but the 5′-UTR sequence diverged 33 bp upstream of the start codon, ATG. Another cDNA clone had a longer 3′-UTR with an additional ˜1KB and a second polyadenylation signal. These sequence differences in the noncoding region indicate that regulation of the use of different polyadenylation signal and/or interaction with different transcription elements of the 5′-UTR could play a role in expression of PN3 in distinct subsets of sensory neurons.
Although the foregoing invention has been described in some detail by way of illustration and example for purposes of clarity and understanding, it will be obvious that certain changes and modifications may be practiced within the scope of the appended claims.
This application is a divisional of U.S. Ser. No. 09/527,013, filed on Mar. 16, 2000, now U.S. Pat. No. 6,479,259, which is a divisional of U.S. Ser. No. 08/843,417, filed on Apr. 15, 1997, now U.S. Pat. No. 6,184,349, which is a continuation-in-part of U.S. Ser. No. 08/511,828, filed on Oct. 11, 1995, now abandoned, all of which are incorporated by reference herein.
| Number | Name | Date | Kind |
|---|---|---|---|
| 5132296 | Cherksey | Jul 1992 | A |
| 5380836 | Rogart | Jan 1995 | A |
| Number | Date | Country |
|---|---|---|
| WO 9614077 | May 1996 | WO |
| WO 9701577 | Jan 1997 | WO |
| WO 9927097 | Jun 1999 | WO |
| Number | Date | Country | |
|---|---|---|---|
| 20030096360 A1 | May 2003 | US |
| Number | Date | Country | |
|---|---|---|---|
| Parent | 09527013 | Mar 2000 | US |
| Child | 10209776 | US | |
| Parent | 08843417 | Apr 1997 | US |
| Child | 09527013 | US |
| Number | Date | Country | |
|---|---|---|---|
| Parent | 08511828 | Oct 1995 | US |
| Child | 08843417 | US |
