Patent Application
20240210435
The present disclosure relates generally to the field of sample preparation and testing, more particularly relates to methods, compositions, systems, and apparatuses for preparing and testing biological samples to aid, for example, in environmental, agricultural, scientific, veterinary or medical diagnosis based on detection of the presence or absence of specific analytes in a sample and/or determining their quantities in the sample.
The amplification of nucleic acids is important in many fields, including medical, biomedical, environmental, veterinary and food safety testing. Nucleic acids can be amplified by polymerase chain reaction (PCR) or isothermal amplification. After DNA amplification there will be a large number of copies of the target genetic sequences in the test solution. In a diagnostic test assay, specific markers can be designed that will link to the target sequences, and once bonded provide an optical signal or optical change that can be detected external to the test tube. This optical signal may be a change in the color and/or opacity of the sample as measured by a change in the optical absorption of the sample at specific optical wavelengths. The output signal may also be by way of direct light output from the sample, where the marker, when activated by target bonding event, triggers release of bioluminescence light output. The optical detection output may also be by a change in the fluorescence of the solution, which may be from a fluorescence marker beacon. In this case, each marker molecule can be configured with a florescence quencher in close proximity to a fluorescence atom or arrangement of atoms. This marker molecule can be configured such that when it selectively binds to a target DNA sequence in the test solution, the quencher and fluorophore are separated and a strong fluorescence signal can then be detected by the action of the fluorophore. In this arrangement, the overall florescence intensity of the target solution is indicative of the relative amount of target generic material in the test solution. This signal can then be used to form the basis of a diagnostic test to determine the presence or absence and the relative quantity of the target material in the sample under test.
Current sample testing systems and apparatuses, in particular nucleic acid amplification and detection instruments, are typically large, complex and costly, and require sample preparation steps that must be conducted independently of the instrument. These preparation steps typically require a trained technical operator, and this operator and the test preparation environment can be exposed to hazardous samples such as body fluids and infectious agents, and the process is at risk from incorrect manual operations, including spills and incorrect reagent additions. The resulting test sample must then be accurately subsampled and transferred by a manual transfer step, typically a skilled pipetting operation. This approach requires a trained technical operator and a number of separate tubes and transfer devices, all of which will be contaminated by the sample and must be correctly handled and disposed of individually. In these approaches, the test sample is not scaled from the environment during the process of sample preparation and transfer into test tubes in the test instrument. This exposure to the sample can present infection agent risks to users and others, and can also contaminate the test instrument and test area, resulting in incorrect diagnostic results in subsequent tests.
An alternative approach involves a cartridge body (e.g., sample preparation reservoir) reliably retaining sample preparation solution therein until such time as a sub-volume (e.g., a predetermined sub-volume of the sample fluid from the cartridge body) is dispensed through perforations in the otherwise scaled wall between the cartridge body and coupled reaction chambers (e.g., test tube(s)). However in order to achieve the desired performance of the chemistry, the amount of sample fluid dispensed into the reaction chambers from the cartridge body must be accurate within a small tolerance. There is a need for methods, compositions, systems, and apparatuses for improving the flow and accuracy of the dispensed sample fluid from a cartridge body into said reaction chamber(s).
Disclosed herein include sample testing systems. In some embodiments, the sample testing system comprises: a cartridge body to receive a biological or environmental sample into a sample preparation fluid contained in the cartridge body for preparation of a sample fluid therefrom. In some embodiments, the sample testing system comprises: at least one reaction chamber coupled to the cartridge body. In some embodiments, the sample testing system comprises: at least one seal between the cartridge body and the at least one reaction chamber to prevent fluid movement between the cartridge body and the at least one reaction chamber. In some embodiments, the sample testing system comprises: a sample dispensing mechanism for insertion into the cartridge body (e.g., after receipt of the biological or environmental sample therein). The sample testing system can comprise at least one seal to prevent fluid movement between the cartridge body and the at least one reaction chamber. In some embodiments, the at least one seal is situated between the cartridge body and the at least one reaction chamber. In some embodiments, the at least one seal is capable of being punctured by a piercing tip to thereby enable fluid movement between the cartridge body and the at least one reaction chamber.
In some embodiments, the sample dispensing mechanism is operable to disrupt the at least one seal to allow sample fluid to enter the at least one reaction chamber from the cartridge body, and to dispense a predetermined sub-volume of the sample fluid from the cartridge body into the at least one reaction chamber for testing therein while preventing further fluid movement between the cartridge body and the at least one reaction chamber. In some embodiments, the sample dispensing mechanism comprises a dispense rod comprising at least one piercing tip that disrupts the at least one seal by forming at least one opening therein, and wherein the at least one piercing tip comprises a geometry configured to generate a large opening in the at least one seal. In some embodiments, the cartridge body initially provides an open volume free of obstructions such that a swab carrying the biological or environmental sample can be used to stir the sample preparation fluid in the cartridge body and to wash the biological or environmental sample from the swab into the sample preparation fluid.
The sample testing system can comprise: a closure to seal the cartridge body after receipt of the biological or environmental sample and the sample dispensing mechanism therein. In some embodiments, at least one of the closure and the cartridge body is configured to prevent or at least inhibit removal of the closure from the cartridge body so that the fluids remain scaled within the sample testing system. In some embodiments, the sample dispensing mechanism is attached to the closure so that an act of applying the closure to the cartridge body also effects the insertion of the sample dispensing mechanism into the cartridge body. The seal can prevent fluid movement prior to the seal puncture. In some embodiments, the shape of the cartridge body prevents rotation when screwing the cap on, and orients to a specific position the plurality of reaction chambers. In some embodiments, a single action by a user causes the sample dispensing mechanism to disrupt the at least one seal and to dispense the sample fluid from the cartridge body into the at least one reaction chamber. In some embodiments, the single action by the user is a sustained screwing action applied to the closure relative to the cartridge body, and wherein the screwing action causes operation of the sample dispensing mechanism and seals the cartridge body. In some embodiments, the closure comprises a screw thread. The sample testing system can comprise: a second closure that seals the sample preparation fluid within the cartridge body prior to use, and that is removed to allow the biological or environmental sample to be added to the sample preparation fluid contained in the cartridge body. In some embodiments, the single action by the user is a downward force applied to the closure relative to the cartridge body. In some embodiments, the downward force forms a snap fit between the closure and the cartridge body. In some embodiments, the downward force causes operation of the sample dispensing mechanism and seals the cartridge body. In some embodiments, the downward force comprises the downward force of a lever means. In some embodiments, the closure comprises a snap-fit dispense cap comprising one or more snapping members configured to form a snap fit with a distal end of the cartridge body upon the single action by the user.
In some embodiments, the sample dispensing mechanism comprises: a dispensing chamber that forms a second seal against the at least one seal to trap the predetermined sub-volume of the sample fluid within the dispensing chamber; and a plunger mechanism that forms a sliding seal with an internal surface of the dispensing chamber, wherein the sliding seal is configured to slide along the internal surface of the dispensing chamber to dispense the predetermined sub-volume of the sample fluid therefrom, through the at least one opening, and into the at least one reaction chamber. In some embodiments, the dispensing chamber comprises an outer surface having mutually spaced chamber locating features extending therefrom and configured to align the dispensing chamber centrally of the cartridge body and allow sample fluid to flow between the chamber locating features as the sample dispensing mechanism is inserted into the cartridge body.
In some embodiments, the sample dispensing mechanism is configured so that a single action performed by a user causes two stages of operation of the sample dispensing mechanism, including a first stage of operation that traps the predetermined sub-volume of the sample fluid within the dispensing chamber, and a second stage of operation wherein the sample fluid is dispensed from the dispensing chamber. In some embodiments, the sample dispensing mechanism comprises a force sequencing component that is reconfigured or broken to allow the second stage of operation. In some embodiments, the force sequencing component comprises a breakable component that is configured to break to allow operation of the sample dispensing mechanism to proceed from the first stage of operation to the second stage of operation. In some embodiments, the force sequencing component comprises a collapsible or crushable spacer that presses against and causes the dispensing chamber to seal in the first stage of operation, and in the second stage of operation is collapsed or crushed to maintain the seal, perform the perforation action, and operate the plunger to dispense the sample fluid from the dispensing chamber.
In some embodiments, the at least one piercing tip comprises a ball point tip. In some embodiments, the at least one piercing tip comprises an arrow head tip. In some embodiments, the at least one piercing tip comprises a frustoconical tip. In some embodiments, the at least one piercing tip does not comprise a sharp tip. In some embodiments, the distal portion of the at least one piercing tip comprises a flat surface. In some embodiments, the flat surface is at an angle of less than about 20°, about 15°, about 10°, about 5°, or about 1°, relative to the surface of the at least one seal.
In some embodiments, the at least one piercing tip is fluted. In some embodiments, the at least one piercing tip comprises one or more flow channels. In some embodiments, the one or more flow channels are positioned at (i) the proximal end of the at least one piercing tip, (ii) the distal end of the at least one piercing tip, or (iii) across the length of the at least one piercing tip. In some embodiments, at least a portion of the predetermined sub-volume of the sample fluid flows through the at least one opening via the one or more flow channels. In some embodiments, the fluid flow is at a higher flow rate as compared to a sample testing system wherein the least one piercing tip does not comprise one or more flow channels. In some embodiments, the one or more flow channels comprise a longitudinal groove extending along the at least one piercing tip. In some embodiments, the at least one piercing tip disrupting the at least one seal comprises the at least one piercing tip penetrating the at least one seal and moving into at least a portion of the at least one reaction chamber. In some embodiments, the at least one opening grows larger in size as the at least one piercing tip moves into at least a portion of the at least one reaction chamber. In some embodiments, the at least one opening stays substantially the same size as the at least one piercing tip moves into at least a portion of the at least one reaction chamber.
In some embodiments, the at least one reaction chamber comprises trapped gas, wherein the cartridge body comprises a gaseous head space above the sample fluid. In some embodiments, the dispense rod is configured to equalize pressure between the gaseous head space and the at least one reaction chamber after the at least one seal is disrupted. In some embodiments, the at least one piercing tip comprises at least one vent opening leading to a vent lumen extending through the dispense rod, wherein the dispense rod comprises a vent port positioned in the gaseous headspace and in fluid communication with the vent lumen of the dispense rod. In some embodiments, the sample dispensing mechanism comprises at least one hydrophobic filter. In some embodiments, any fluid passing between the at least one vent port and the at least one vent opening must pass through the hydrophobic filter. In some embodiments, the vent opening is positioned at (i) the proximal end of the at least one piercing tip, (ii) the distal end of the at least one piercing tip, or (iii) across the length of the at least one piercing tip. In some embodiments, the trapped gas displaced by the at least one piercing tip and/or the predetermined sub-volume of the sample fluid is capable of escaping via the at least one vent opening to the gaseous head space.
In some embodiments, the at least one piercing tip disrupting the at least one seal is capable of generating one or more flaps, wherein the one or more flaps comprise portion(s) of the at least one seal disrupted by the at least one piercing tip. In some embodiments, the flaps do not adhere to the at least one piercing tip and/or do not disrupt fluid flow through the opening. In some embodiments, the piercing tip comprises a geometry configured to reduce wicking of the sample fluid to the at least one piercing tip and/or the one or more flaps.
The large opening can comprise, for example, a puncture of at least about 50%, about 55%, about 60%, about 65%, about 70%, about 75%, about 80%, about 85%, about 90%, about 95%, about 98%, or about 99%, of the surface area of the at least one seal. In some embodiments, at least about 50%, about 55%, about 60%, about 65%, about 70%, about 75%, about 80%, about 85%, about 90%, about 95%, about 98%, or about 99%, of the surface area of the at least one seal comes into contact with the at least one piercing tip. In some embodiments, at least about 70%, about 75%, about 80%, about 85%, about 90%, about 95%, about 98%, or about 99%, of the predetermined sub-volume of the sample fluid enters the at least one reaction chamber. In some embodiments, less than about 30%, about 25%, about 20%, about 15%, about 10%, about 5%, or about 1%, of the predetermined sub-volume of the sample fluid remains in or on the dispensing chamber, the at least one seal, and/or the at least one piercing tip after the at least one seal is disrupted. In some embodiments, the predetermined sub-volume of the sample fluid comprises at least about 10 μL, about 15 μL, about 20 μL, about 25 μL, about 30 μL, about 35 μL, about 40 μL, about 45 μL, about 50 μL, about 60 μL, about 70 μL, about 80 μL, about 90 μL, about 100 μL, about 110 μL, about 120 μL, about 128 μL, about 130 μL, about 140 μL, about 150 μL, about 160 μL, about 170 μL, about 180 μL, about 190 μL, or about 200 μL, of the sample fluid.
In some embodiments, the sample dispensing mechanism comprises an overmolded layer disposed on the surface of at least a portion of the dispense rod and/or the dispensing chamber. In some embodiments, the overmolded layer forms a seal. In some embodiments, a cylindrical seal. In some embodiments, the overmolded layer comprises a thermoplastic elastomer (TPE) of a different durometer than at least a portion of the dispense rod and/or the dispensing chamber. In some embodiments, the overmolded layer exhibits a Shore D durometer or a Shore A durometer of about 20-30.
In some embodiments, the dispensing chamber is initially configured so that, as the sample dispensing mechanism is inserted into the cartridge body, the sample fluid is forced to flow around the outside of the dispensing chamber before it can flow into the dispensing chamber, wherein the fluid that flows around the outside of the dispensing chamber is caused to flow through a filter or porous filler material that retains and/or traps particles and debris and/or incorporates biological or chemical components that bind to or capture components of the sample fluid that may otherwise inhibit or interfere with the sample testing.
In some embodiments, the cartridge body comprises one or more magnetic particles with the sample preparation fluid, the surface of the magnetic particles being coated or functionalized to bind with and capture at least one predetermined target species of the biological or environmental sample when the magnetic particles are mixed within the sample fluid, and the sample dispensing mechanism is configured so that, as the sample dispensing mechanism is inserted into the cartridge body, the sample fluid is forced to flow through the dispensing chamber, and one or more magnets are located in close proximity to the inside surface of the dispensing chamber so that magnetic particles contained within the sample fluid and have captured target species are attracted to and held against the internal surface of the dispensing chamber, such that the plunger mechanism that forms a sliding seal with the internal surface of the dispensing chamber collects the magnetic particles held against the internal surface and dispenses them into the at least one reaction chamber to provide an increased concentration of the at least one predetermined target species in the predetermined sub-volume of the sample fluid dispensed into the at least one reaction chamber.
In some embodiments, the at least one reaction chamber is two reaction chambers, and wherein the at least one piercing tip is two piercing tips. In some embodiments, the reaction chambers comprise polymerase chain reaction (PCR) tubes. In some embodiments, the two reaction chambers comprise a mixing bead. In some embodiments, the reaction chambers comprise different reagents selected to perform respective different tests and/or to detect respective different target entities. In some embodiments, the cartridge body comprises sample preparation reagents, and wherein at least one of the reaction chambers comprises one or more reagents for a reverse transcription reaction and/or an amplification reaction. In some embodiments, the cartridge body comprises one or more alignment features configured to align and engage with one or more mating slots of a testing apparatus. In some embodiments, the one or more alignment features prevent rotation of the cartridge body when it is in place in the testing apparatus. In some embodiments, the one or more alignment features enable a user to remove the second closure and/or perform the single action in a single-handed operation.
Disclosed herein include sample testing methods. In some embodiments, sample testing method comprises the steps of: adding a biological or environmental sample into a sample preparation fluid contained in a cartridge body of a sample testing system disclosed herein for preparation of a sample fluid therein; after the adding step, inserting a sample dispensing mechanism into the cartridge body and applying a closure thereto; and operating the sample dispensing mechanism to disrupt at least one seal between the cartridge body and at least one reaction chamber to allow sample fluid to enter the at least one reaction chamber from the cartridge body, and to dispense a predetermined sub-volume of the sample fluid from the cartridge body into the at least one reaction chamber for testing therein while preventing further fluid movement between the cartridge body and the at least one reaction chamber. The method can comprise: before the adding step, placing the sample testing system into a receiving port of a testing apparatus configured to perform a test on the biological or environmental sample therein.
There are provided, in some embodiments, testing apparatuses. A testing apparatus can comprise a receiving port configured to receive a sample testing system provided herein. In some embodiments, the testing apparatus is configured to perform a test on the biological or environmental sample therein. In some embodiments, the testing apparatus further comprises a lever means configured to apply a downward force to the sample testing system placed in the receiving port. In some embodiments, the single action is a downward force applied to the closure relative to the cartridge body via the lever means. In some embodiments, the downward force forms a snap fit between the closure and the cartridge body. In some embodiments, the lever means comprises a hinged lid. In some embodiments, the hinged lid comprises a ridge configured to contact the surface of the closure. In some embodiments, the testing apparatus further comprises a sleeve for storing the lever means. In some embodiments, the hinged lid is substantially parallel to the cartridge body when stored within the sleeve. In some embodiments, at least a portion of the hinged lid is configured to slide up and out of the sleeve when lifted by a user to expose a hinge of the hinged lid. In some embodiments, upon the hinge being exposed, the hinged lid is capable of being pivoted into a horizontal position substantially perpendicular to the cartridge body. In some embodiments, the testing apparatus comprises one or more mating slots configured to align and engage with one or more alignment features of a cartridge body. In some embodiments, the one or more mating slots are situated in the receiving port. In some embodiments, the one or more alignment features prevent rotation of the cartridge body when it is in place in the testing apparatus. In some embodiments, the one or more alignment features enable a user to remove the second closure and/or perform the single action in a single-handed operation.
In the following detailed description, reference is made to the accompanying drawings, which form a part hereof. In the drawings, similar symbols typically identify similar components, unless context dictates otherwise. The illustrative embodiments described in the detailed description, drawings, and claims are not meant to be limiting. Other embodiments may be utilized, and other changes may be made, without departing from the spirit or scope of the subject matter presented herein. It will be readily understood that the aspects of the present disclosure, as generally described herein, and illustrated in the Figures, can be arranged, substituted, combined, separated, and designed in a wide variety of different configurations, all of which are explicitly contemplated herein and made part of the disclosure herein.
All patents, published patent applications, other publications, and sequences from GenBank, and other databases referred to herein are incorporated by reference in their entirety with respect to the related technology.
Disclosed herein include sample testing systems. The sample testing system can comprise: a cartridge body to receive a biological or environmental sample into a sample preparation fluid contained in the cartridge body for preparation of a sample fluid therefrom. In some embodiments, the sample testing system comprises: at least one reaction chamber coupled to the cartridge body. In some embodiments, the sample testing system comprises: at least one seal between the cartridge body and the at least one reaction chamber to prevent fluid movement between the cartridge body and the at least one reaction chamber. In some embodiments, the sample testing system comprises: a sample dispensing mechanism for insertion into the cartridge body after receipt of the biological or environmental sample therein.
In some embodiments, the sample dispensing mechanism is operable to disrupt the at least one seal to allow sample fluid to enter the at least one reaction chamber from the cartridge body, and to dispense a predetermined sub-volume of the sample fluid from the cartridge body into the at least one reaction chamber for testing therein while preventing further fluid movement between the cartridge body and the at least one reaction chamber. In some embodiments, the sample dispensing mechanism comprises a dispense rod comprising at least one piercing tip that disrupts the at least one seal by forming at least one opening therein, and wherein the at least one piercing tip comprises a geometry configured to generate a large opening in the at least one seal. In some embodiments, the cartridge body initially provides an open volume free of obstructions such that a swab carrying the biological or environmental sample can be used to stir the sample preparation fluid in the cartridge body and to wash the biological or environmental sample from the swab into the sample preparation fluid.
Disclosed herein include sample testing methods. The sample testing method can comprise, for example, the steps of: adding a biological or environmental sample into a sample preparation fluid contained in a cartridge body of a sample testing system disclosed herein for preparation of a sample fluid therein; after the adding step, inserting a sample dispensing mechanism into the cartridge body and applying a closure thereto; and operating the sample dispensing mechanism to disrupt at least one seal between the cartridge body and at least one reaction chamber to allow sample fluid to enter the at least one reaction chamber from the cartridge body, and to dispense a predetermined sub-volume of the sample fluid from the cartridge body into the at least one reaction chamber for testing therein while preventing further fluid movement between the cartridge body and the at least one reaction chamber. The method can comprise: before the adding step, placing the sample testing system into a receiving port of a testing apparatus configured to perform a test on the biological or environmental sample therein.
Unless defined otherwise, technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which the present disclosure belongs. See, e.g. Singleton et al., Dictionary of Microbiology and Molecular Biology 2nd ed., J. Wiley & Sons (New York, NY 1994); Sambrook et al., Molecular Cloning, A Laboratory Manual, Cold Spring Harbor Press (Cold Spring Harbor, NY 1989). For purposes of the present disclosure, the following terms are defined below.
There are provided, in some embodiments, methods, compositions, systems, and apparatuses for sample testing. Disclosed herein include methods, compositions, systems, and apparatuses for piercing seals for patient sample assays and piercing sample assays. There is provided, in some embodiments, a dual rod piercing and fluid dispensing device. The device can be part of the consumable of a molecular point of care rapid test system. The consumable device can be composed of a two part system. The first part of the system can comprise the main cartridge, composed of a cap, an upper section called the cartridge body (e.g., sample preparation reservoir) filled with a diluent (e.g., sample preparation fluid), and a lower section called the dual tube which is a symmetrical pair of reaction chambers each containing dried down molecular reagents and a mixing bead, the upper and lower sections being separated by a foil seal and an elastomeric gasket. The second part of the system can comprise a dispense rod and/or cap assembly.
In some embodiments of the uses provided herein, a patient sample, typically collected with a swab, is mixed into the diluent in the cartridge. The swab can be removed, and then the dispense rod can be inserted into the cartridge. In some embodiments, screwing the cap of the dispense rod and cap assembly drives the dispense rod downward through the cartridge body, pierces the foil seal directly above each of the two reaction chambers, and drives the sample fluid through the seal and into the reaction chambers. In order to achieve the desired performance of the chemistry, the amount of fluid dispensed into the reaction chambers must be accurate within a small tolerance.
There are provided, in some embodiments, dispense rods configured to improve the flow and accuracy of the dispensed fluid. Various piercing tip geometries are provided herein that have at their central purpose creating a larger opening in the foil and creating a higher volume pathway through which to direct the liquid flow.
Currently available compositions and methods suffer from the cartridge design disadvantage that the liquid is forced into the scaled reaction chambers, compressing the gas in the chambers and creating pressure on the seals, increasing the opportunities for leaks to occur, which in turn reduces the accuracy of the dispensed volume. Embodiments provided herein contemplate various geometries to allow the gas in the reaction chamber to vent into the head space of the cartridge body, equalizing the pressure and thereby improving the reliability of the seals and the accuracy of the dispensed volume. Provided herein, in some embodiments, is a complex plastic disposable that pierces foil to push patient sample for assay and deliver dispense a predetermined sub-volume of the sample fluid (e.g., 100 μL) from the cartridge body into the at least one reaction chamber of a reagent dual tube region.
In some embodiments, the piercing tip geometry is such that a full opening is created to allow a full dispense to the reaction chambers. In some embodiments, the piercing tip geometry also is designed such to limit wicking of aliquot and/or vent trapped gas in the reagent chamber. Embodiments of the dispense rod and piercing tip geometry provided herein can be combined, e.g., adding a geometry to allow venting of trapped gas to increase accuracy of the dispensed aliquot. In some embodiments, the tip geometry provided herein allows for a greater puncture orifice for better volume dispense as well possible venting of gas trapped in an enclosed chamber.
A sharp tip can pierce a seal such as an AL seal, but can then close back round the shaft cause poor flow and adhesion of fluid. The ball point tip of the piercing tips described herein can allow a larger puncture to increase fluid flow and prevent the seal such as foil to want to grab onto the shaft of the dispensing rod. Fluted tip designs of the piercing tips described herein can also allow a larger puncture and at the same time vent and allow fluid to flow via the flutes. Arrow head designs of the piercing tips described herein can also improve fluid flow and increase accuracy of the dispensed aliquot.
Foil piercing rods with unique geometries to enhance fluid dispensing and dual piercing dispense rods for aliquoting are provided herein. There are provided, in some embodiments, PCR foil dispense rods. The methods, compositions, systems, and apparatuses provided herein, such as the dispensing rods disclosed herein, can be employed in a variety of dispensing contexts beyond PCR tubes. The compositions, systems, and methods described herein find utility in a variety of different environments where a predetermined sub-volume of the sample fluid is dispensed into a chamber (e.g., reaction chamber).
Without being bound by any particular theory, the methods, compositions, systems, and apparatuses disclosed herein yield improved performance relative to currently available methods and systems due to the improved fluid flow, reduced fluid adhesion, and/or venting of trapped gas of the embodiments provided herein.
Disclosed herein include sample testing systems. The sample testing system can comprise: a cartridge body to receive a biological or environmental sample into a sample preparation fluid contained in the cartridge body for preparation of a sample fluid therefrom. The sample testing system can comprise: at least one reaction chamber coupled to the cartridge body. The sample testing system can comprise: at least one seal between the cartridge body and the at least one reaction chamber to prevent fluid movement between the cartridge body and the at least one reaction chamber. The sample testing system can comprise: a sample dispensing mechanism for insertion into the cartridge body after receipt of the biological or environmental sample therein.
The sample dispensing mechanism can be operable to disrupt the at least one seal to allow sample fluid to enter the at least one reaction chamber from the cartridge body, and to dispense a predetermined sub-volume of the sample fluid from the cartridge body into the at least one reaction chamber for testing therein while preventing further fluid movement between the cartridge body and the at least one reaction chamber. The sample dispensing mechanism can comprise a dispense rod comprising at least one piercing tip that disrupts the at least one seal by forming at least one opening therein. The at least one piercing tip can comprise a geometry configured to generate a large opening in the at least one seal. The large opening can comprise a puncture of at least about 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59%, 60%, 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69%, 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 100%, or a number or a range between any two of these values, of the surface area of the at least one seal. In some embodiments, the cartridge body initially provides an open volume free of obstructions such that a swab carrying the biological or environmental sample can be used to stir the sample preparation fluid in the cartridge body and to wash the biological or environmental sample from the swab into the sample preparation fluid.
The sample dispensing mechanism can comprise: a dispensing chamber that forms a second seal against the at least one seal to trap the predetermined sub-volume of the sample fluid within the dispensing chamber. The sample dispensing mechanism can comprise: a plunger mechanism that forms a sliding seal with an internal surface of the dispensing chamber, wherein the sliding seal is configured to slide along the internal surface of the dispensing chamber to dispense the predetermined sub-volume of the sample fluid therefrom, through the at least one opening, and into the at least one reaction chamber. The dispensing chamber can comprise an outer surface having mutually spaced chamber locating features extending therefrom and configured to align the dispensing chamber centrally of the cartridge body and allow sample fluid to flow between the chamber locating features as the sample dispensing mechanism is inserted into the cartridge body.
The sample dispensing mechanism can be configured so that a single action performed by a user causes two stages of operation of the sample dispensing mechanism, including a first stage of operation that traps the predetermined sub-volume of the sample fluid within the dispensing chamber, and a second stage of operation wherein the sample fluid is dispensed from the dispensing chamber. The sample dispensing mechanism can comprise a force sequencing component that is reconfigured or broken to allow the second stage of operation. The force sequencing component can comprise a breakable component that is configured to break to allow operation of the sample dispensing mechanism to proceed from the first stage of operation to the second stage of operation. The force sequencing component can comprise a collapsible or crushable spacer that presses against and causes the dispensing chamber to seal in the first stage of operation, and in the second stage of operation is collapsed or crushed to maintain the seal, perform the perforation action, and operate the plunger to dispense the sample fluid from the dispensing chamber.
The at least one piercing tip can comprise a ball point tip. The at least one piercing tip can comprise an arrow head tip. The at least one piercing tip can comprise a frustoconical tip.
Some embodiments of the sample testing systems provided herein comprise one or more overmolded layers. One or more components of the cartridges provided herein can comprise a thermoplastic elastomer (TPE). Cartridges are provided herein that comprise one or more overmolded layers of different durometer. The choice of TPE and the durometer thereof can vary depending on the embodiment and the nature and purpose of the overmolded layer. In some embodiments, the sample dispensing mechanism comprises an overmolded layer disposed on the surface of at least a portion of the dispense rod and/or the dispensing chamber. The overmolded layer can form a seal, such as a cylindrical seal. The overmolded layer can comprise a thermoplastic elastomer (TPE) of a different durometer than a surface which it covers, such as, for example, at least a portion of the dispense rod and/or the dispensing chamber. The durometer of the TPE can be different in different embodiments. In some embodiments, the durometer of the TPE can be, or can be about, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, or a number or a range between any two of these values, on the Shore A scale or on the a Shore D scale. In some embodiments, the durometer of the TPE can be at least, or can be at most, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, on the Shore A scale or on the a Shore D scale. In some embodiments, the overmolded layer exhibits a Shore D durometer or a Shore A durometer of about 20-30.
A dispense rod can comprise at least one piercing tip. The number of piercing tips can vary. The number of piercing tips can correspond to the number of reaction chambers in the cartridge. For example, a dual reaction chamber cartridge can comprise a dispense rod with dual action piercing tips. The number of piercing tips on a dispense rod can be different in different embodiments. In some embodiments, the number of piercing tips on a dispense rod can be, or can be about, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 200, 300, 400, 500, 600, 700, 800, 900, 1000, or a number or a range between any two of these values. In some embodiments, the number of piercing tips on a dispense rod can be at least, or can be at most, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 200, 300, 400, 500, 600, 700, 800, 900, or 1000. The number of reaction chambers coupled to the cartridge body can be different in different embodiments. In some embodiments, the number of reaction chambers coupled to the cartridge body can be, or can be about, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 200, 300, 400, 500, 600, 700, 800, 900, 1000, or a number or a range between any two of these values. In some embodiments, the number of reaction chambers coupled to the cartridge body can be at least, or can be at most, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 200, 300, 400, 500, 600, 700, 800, 900, or 1000. In some embodiments, the number of seals can be at least, or can be at most, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 200, 300, 400, 500, 600, 700, 800, 900, or 1000.
The volume of the predetermined sub-volume of the sample fluid can be different in different embodiments. The predetermined sub-volume of the sample fluid comprises at least about 10 μL, about 15 μL, about 20 μL, about 25 μL, about 30 μL, about 35 μL, about 40 μL, about 45 μL, about 50 μL, about 60 μL, about 70 μL, about 80 μL, about 90 μL, about 100 μL, about 110 μL, about 120 μL, about 128 μL, about 130 μL, about 140 μL, about 150 μL, about 160 μL, about 170 μL, about 180 μL, about 190 μL, or about 200 μL, or a number or a range between any two of these values.
Reaction chambers coupled to the same cartridge can comprise different reagents selected to perform respective different tests and/or to detect respective different target entities. In some embodiments, the number of different types of reagents can be at least, or be at most, 2, 3, 4, 5, 6, 7, 8, 9, 10, 20, 30, 40, 50, 60, 70, 80, 90, 100, 200, 300, 400, 500, 600, 700, 800, 900, 1000, 10000, or 100000. The reagents can, for example, be lyophilized, heat dried, freeze dried, or in a stable buffer. The test reagents contained within the cartridge prior to test can be configured for other types of testing not necessary utilizing nucleic acid amplification. For example, direct chemical reaction detection can be used in some embodiments to detect the presence of trace elements or additives within a sample. Optionally, immunoassay detection methods can be used to directly bind to and provide detection of specific proteins within the sample material that has been diluted and dispensed into one or more reaction chambers (e.g., test tubes).
The at least one piercing tip disrupting the at least one seal can be capable of generating one or more flaps. The one or more flaps can comprise portion(s) of the at least one seal disrupted by the at least one piercing tip. In some embodiments, the flaps do not adhere to the at least one piercing tip and/or do not disrupt fluid flow through the opening. The piercing tip can comprise a geometry configured to reduce wicking of the sample fluid to the at least one piercing tip and/or the one or more flaps.
In some embodiments, at least about 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59%, 60%, 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69%, 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 100%, or a number or a range between any two of these values, of the surface area of the at least one seal comes into contact with the at least one piercing tip. In some embodiments, at least about 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 100%, or a number or a range between any two of these values, of the predetermined sub-volume of the sample fluid enters the at least one reaction chamber. In some embodiments, less than about 30%, about 25%, about 20%, about 15%, about 10%, about 5%, about 1%, or a number or a range between any two of these values, of the predetermined sub-volume of the sample fluid remains in or on the dispensing chamber, the at least one seal, and/or the at least one piercing tip after the at least one seal is disrupted.
In some embodiments, the at least one piercing tip does not comprise a sharp tip.
The at least one reaction chamber can comprise trapped gas. The cartridge body can comprise a gaseous head space above the sample fluid. The dispense rod can be configured to equalize pressure between the gaseous head space and the at least one reaction chamber after the at least one seal is disrupted. The at least one piercing tip can comprise at least one vent opening leading to a vent lumen extending through the dispense rod. The dispense rod can comprise a vent port positioned in the gaseous headspace and in fluid communication with the vent lumen of the dispense rod. The sample dispensing mechanism can comprise at least one hydrophobic filter. In some embodiments, any fluid passing between the at least one vent port and the at least one vent opening must pass through the hydrophobic filter. The vent opening can be positioned at (i) the proximal end of the at least one piercing tip, (ii) the distal end of the at least one piercing tip, or (iii) across the length of the at least one piercing tip. The trapped gas displaced by the at least one piercing tip and/or the predetermined sub-volume of the sample fluid can be capable of escaping via the at least one vent opening to the gaseous head space.
There are provided, in some embodiments, a (diagnostic) test apparatus (referred to as ‘instrument’), and a sample testing system (also referred to herein for convenience of reference as a ‘cartridge’) for use with the test instrument to perform a test on a biological or environmental sample. The cartridge and instrument described herein can be easy for a user to operate without requiring the facilities of a general test laboratory. Sample testing systems, including diagnostic test assemblies and diagnostic test apparatuses, have been described in U.S. Patent Application Publication No. 2020/0278368, the content of which is incorporated herein by reference in its entirety.
There are provided, in some embodiments, a test cartridge with a removable closure or cap to allow addition of a test sample, where the cartridge incorporates a cartridge body containing a sample preparation fluid such as a buffer or lysis solution to assist with preparation of the sample and can include separation of target DNA material from within the sample cells. The sample preparation fluid reservoir section (e.g., cartridge body) of the cartridge can be a closed volume to reliably retain the sample preparation solution until such time as a sub-volume (e.g., a predetermined sub-volume of the sample fluid from the cartridge body) is dispensed through perforations in the otherwise scaled wall between the reservoir and the coupled reaction chamber(s). Optionally, the cartridge incorporates the chemical and biological reagents required for sample preparation and testing. In some embodiments, said reagents include those configured for nucleic acid amplification, genetic sequence binding and optical output using iso-thermal nucleic acid amplification methods. Optionally, the cartridge incorporates the chemical and biological reagents required for sample preparation and nucleic acid amplification and genetic sequence detection using polymerase chain reaction, PCR, nucleic acid amplification methods.
In some embodiments, the sample testing system is provided in the form of a disposable diagnostic test cartridge that is produced prior to a test (e.g., diagnostic test), and already incorporates all of the precursor chemical components (e.g., reagents) to run a specific set of one or more diagnostic tests. In some embodiments, the sample testing system/cartridge is configured so that it can be safely handled without contamination from the environment, or causing contamination of the user or the environment with the test materials, or causing interference with these chemical components or otherwise affecting the subsequent operations of the cartridge, which can require interactions with an diagnostic test instrument.
A user of the sample testing system wishing to conduct a test on a biological or environmental sample introduces the sample into the cartridge. With its closure removed, at this step the cartridge provides an open volume that is free of obstructions, by which is meant that a swab carrying the biological or environmental sample can easily be used to stir the sample preparation fluid in the cartridge body and to wash the biological or environmental sample from the swab into the sample preparation fluid without encountering obstructions that would impede this step. However, although this characterizes the open volume within the cartridge, it will be apparent to those skilled in the art that it is not necessary that a swab be used at all, and samples in any suitable form can be added to the sample preparation fluid by any suitable means.
The sample testing system can comprise: a closure to seal the cartridge body after receipt of the biological or environmental sample and the sample dispensing mechanism therein. The at least one of the closure and the cartridge body can be configured to prevent or at least inhibit removal of the closure from the cartridge body so that the fluids remain scaled within the sample testing system. The sample dispensing mechanism can be attached to the closure so that an act of applying the closure to the cartridge body also effects the insertion of the sample dispensing mechanism into the cartridge body. In some embodiments, a single action by a user causes the sample dispensing mechanism to disrupt the at least one seal and to dispense the sample fluid from the cartridge body into the at least one reaction chamber. The single action by the user can be a sustained screwing action applied to the closure relative to the cartridge body, and wherein the screwing action causes operation of the sample dispensing mechanism and seals the cartridge body. The closure can comprise a screw thread. The sample testing system can comprise: a second closure that seals the sample preparation fluid within the cartridge body prior to use, and that is removed to allow the biological or environmental sample to be added to the sample preparation fluid contained in the cartridge body.
In the case of biological samples, the step of adding the sample to the sample preparation fluid within the cartridge body initiates a specific biological and chemical process of sample dilution and cell lysis to prepare the sample material, including its included RNA or DNA nucleic acid, for testing. However, the system is not limited to biological tests, and can, for example, be used to detect the presence of or measure the amounts of trace elements in any type of sample. Other suitable types of diagnostic tests will be apparent to those skilled in the art in light of this disclosure.
The cartridge protects the reagents in transport and storage prior to running a test, and supports the test process while the diagnostic test is underway. In some embodiments, the test reagents, amplification genetic products and contaminants are retained within the cartridge at all times, including at the completion of the test. The scaled cartridge can be removed for disposal at the completion of a test, and, in some embodiments, an instrument is protected from fluids and contamination at all times.
After the biological sample is added to and then scaled within the cartridge, the user can be protected from the biological or chemical hazards of the sample during the subsequent test process and after the cartridge is removed for disposal.
The systems provided herein (e.g., diagnostic test cartridge) can include one or more reaction chambers also referred to herein for convenience as ‘test tubes’ close coupled with a separating wall to a cartridge body within the cartridge. In some embodiments, the cartridge body is fully scaled from the coupled reaction chamber(s), and is typically supplied pre-filled with a volume of sample preparation fluid and a removable closure.
In use, the test cartridge can be supported and heated within the test apparatus, and the removable closure is removed to add a sample. In some embodiments, the sample can be any biological or chemical sample for which a suitable diagnostic test and test display chemistry reagents are incorporated within the coupled test tube(s).
In some embodiments, the test cartridge is supplied with an additional cap with an attached dispensing mechanism. In some embodiments, this additional cap incorporates a dispensing mechanism and is fitted after the initial cap has been removed and the sample added. In some embodiments, as the additional cap and dispensing mechanism is inserted and the cap is closed by an action such as screwing it closed, the dispensing mechanism perforates the base of the sample chamber and dispenses a measured volume of prepared sample fluid into the one or more reaction chamber(s). This cap then closes and seals the sample within the cartridge assembly
Alternatively, the first cap, once removed, can have the dispensing mechanism fitted to it, in a separate operation, to forming the additional cap with an included dispensing mechanism ready to be refitted to operate a dispense function and close the cartridge.
Optionally, after the sample is added, the dispensing mechanism itself is directly inserted and then a cap is fitted and the action of closing this cap, such as screwing the cap closed, operates the dispensing mechanism and close the cartridge.
There are provided, in some embodiments, sample testing methods. In some embodiments, sample testing method comprises the steps of: adding a biological or environmental sample into a sample preparation fluid contained in a cartridge body of a sample testing system disclosed herein for preparation of a sample fluid therein; after the adding step, inserting a sample dispensing mechanism into the cartridge body and applying a closure thereto; and operating the sample dispensing mechanism to disrupt at least one seal between the cartridge body and at least one reaction chamber to allow sample fluid to enter the at least one reaction chamber from the cartridge body, and to dispense a predetermined sub-volume of the sample fluid from the cartridge body into the at least one reaction chamber for testing therein while preventing further fluid movement between the cartridge body and the at least one reaction chamber. The method can comprise: before the adding step, placing the sample testing system into a receiving port of a testing apparatus configured to perform a test on the biological or environmental sample therein.
Within a single test well, it is possible to have several different markers present that will provide an optical output based on bonding to several different target genetic DNA sequences. In this case several different sensors are used or a sensor with more than one selective output is used. For example, in a two channel system, two different fluorescence markers may be employed, and these will be detected by two different fluorescence sensors configured to detect emissions in respective frequency ranges specific to the respective fluorescence markers to allow the channels to be discriminated.
Embodiments provided herein can be used to provide a control channel where the test assay chemistry is configured such that the control target should always be present if the test process is run correctly. In this case, the output of the control channel is used to confirm that the test process has been run correctly by the system, and to confirm that test results obtained by other channels measured by the system are valid. Embodiments provided herein can be also used to test for more than one target genetic sequence within each test well as a multiplexed test. Multiple test wells may be used, with each well running differently configured amplification chemistry and a different set of target markers. Control channels may operate in one or more wells and cover tests operated other wells in the test. By this arrangement a number of tests can be conducted on a single sample as a different approach to multiplexing.
Tests (e.g., amplification tests) within a single reaction chamber can be multiplexed in that more than one DNA or RNA target sequence and a control channel can be detected within a single reaction chamber. Where the system uses fluorescence as the detection method, the different targets can be detected with probes that emit at different florescence wavelengths, referred to in the art as detection channels. In an instrument described herein, two channels of detection can be included. Using the single tube (e.g., reaction chamber) cartridge described herein and a two channel detection instrument described herein, the system can provide detection of two different DNA or RNA targets. If additional targets are required to be multiplexed into the single diagnostic test from the same sample, additional reaction chambers can be provided in other embodiments. In this way, additional targets and control channels can be included while using the same number of detection sensors in the instrument. For example, in the case of an embodiment with two instrument sensor channels and two reaction chambers, the system is capable of 4 independent channels of DNA or RNA detection from a single sample that is prepared and dispensed from the cartridge body into the two reaction chambers. There are provided, in some embodiments, cartridges comprising one or more reaction chambers (e.g., test tubes). The number of reaction chambers per cartridge can vary, and can be about, can be at least, or can be at most, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 20, 30, 40, 50, 60, 70, 80, 90, 100, or a number or a range between any of these values. The amplifying step can comprise multiplex amplification of two or more target nucleic acid sequences. The detecting step can comprise multiplex detection of two more nucleic acid amplification products derived from said two or more target nucleic acid sequences. The two or more target nucleic acid sequences can be specific to two or more different organisms. The at least one reaction chamber can be two reaction chambers. The at least one piercing tip can be two or more piercing tips. The reaction chambers can comprise polymerase chain reaction (PCR) tubes. The two reaction chambers can comprise one or more a mixing bead. The reaction chambers can comprise different reagents selected to perform respective different tests and/or to detect respective different target entities. The cartridge body can comprise sample preparation reagents. At least one of the reaction chambers can comprise one or more reagents for a reverse transcription reaction and/or an amplification reaction.
The reaction chamber(s) can comprise one or more reagents, such as, for example, amplification reagents and nucleic acid detection reagents. Components of an amplification reaction (e.g., the one or more amplification reagents) may include, for example, one or more primers (e.g., individual primers, primer pairs, primer sets, oligonucleotides, multiple primer sets for multiplex amplification, and the like), nucleic acid target(s) (e.g., target nucleic acid from a sample), one or more polymerases, nucleotides (e.g., dNTPs and the like), and a suitable buffer (e.g., a buffer comprising a detergent, a reducing agent, monovalent ions, and divalent ions). An amplification reaction may further include a reverse transcriptase and/or a reverse transcription primer, in some embodiments. An amplification reaction may further include one or more detection agents, such as one or more of the detection agents described herein, in some embodiments. In some embodiments, the one or more amplification reagents comprise, or consist of, primers, target nucleic acid, a polymerase, nucleotides, and a suitable buffer. In some embodiments, the one or more amplification reagents comprise, or consist of, primers, target nucleic acid, a polymerase, a reverse transcriptase, a reverse transcription primer, nucleotides, and a suitable buffer. In some embodiments, the one or more amplification reagents consist of primers, target nucleic acid, a polymerase, a detection agent, nucleotides, and a suitable buffer. In some embodiments, the one or more amplification reagents comprise, or consist of, primers, target nucleic acid, a polymerase, a reverse transcriptase, a reverse transcription primer, a detection agent, nucleotides, and a suitable buffer. In some embodiments, the one or more amplification reagents consist essentially of primers, target nucleic acid, a polymerase, nucleotides, and a suitable buffer. In some embodiments, the one or more amplification reagents consist essentially of primers, target nucleic acid, a polymerase, a reverse transcriptase, a reverse transcription primer, nucleotides, and a suitable buffer. In some embodiments, the one or more amplification reagents consist essentially of primers, target nucleic acid, a polymerase, a detection agent, nucleotides, and a suitable buffer. In some embodiments, the one or more amplification reagents consist essentially of primers, target nucleic acid, a polymerase, a reverse transcriptase, a reverse transcription primer, a detection agent, nucleotides, and a suitable buffer. When the one or more amplification reagents consist essentially of certain components, additional components or features may be included that do not have a significant effect on the amplification and/or are not necessary for generating a detectable product. For example, additional components or features may be included that do not have a significant effect on the ability of the components and conditions herein to achieve amplification under isothermal conditions and generate a detectable amplification product within about 10 minutes or less. Such additional components or features may be referred to as non-essential components and may include typical reaction components and/or common additives such as salts, buffers, detergents, ions, oils, proteins, polymers and the like. In some embodiments, amplification conditions comprise an enzymatic activity. Typically, an enzymatic activity is provided by a polymerase, and in some embodiments, an enzymatic activity is provided by a polymerase and a reverse transcriptase. In some embodiments, an enzymatic activity consists of a polymerase activity. In some embodiments, an enzymatic activity consists of a polymerase activity and a reverse transcriptase activity. Accordingly, in some embodiments, enzymatic activity does not include enzymatic activity provided by other enzymes, for example, helicases, topoisomerases, ligases, exonucleases, endonucleases, restriction enzymes, nicking enzymes, recombinases, and the like. In some embodiments, a polymerase activity and a reverse transcriptase activity are provided by separate enzymes or separate enzyme types (e.g., polymerase(s) and reverse transcriptase(s)). In some embodiments, a polymerase activity and a reverse transcriptase activity are provided by a single enzyme or enzyme type (e.g., polymerase(s)). In some embodiments, the amplification comprises one or more of the following: loop-mediated isothermal Amplification (LAMP), helicase-dependent Amplification (HDA), recombinase polymerase amplification (RPA), strand displacement amplification (SDA), nucleic acid sequence-based amplification (NASBA), transcription mediated amplification (TMA), nicking enzyme amplification reaction (NEAR), rolling circle amplification (RCA), multiple displacement amplification (MDA), Ramification (RAM), circular helicase-dependent amplification (cHDA), single primer isothermal amplification (SPIA), signal mediated amplification of RNA technology (SMART), self-sustained sequence replication (3SR), genome exponential amplification reaction (GEAR) and isothermal multiple displacement amplification (IMDA).
In some embodiments, the one or more amplification reagents may include non-enzymatic components and enzymatic components. Non-enzymatic components may include, for example, primers, nucleotides, buffers, salts, reducing agents, detergents, and ions; and generally do not include proteins (e.g., nucleic acid binding proteins), enzymes, or proteins having enzymatic activity, for example, polymerases, reverse transcriptases, helicases, topoisomerases, ligases, exonucleases, endonucleases, restriction enzymes, nicking enzymes, recombinases and the like. In some embodiments, an enzymatic component may consist of a polymerase or may consist of a polymerase and a reverse transcriptase. Accordingly, such enzymatic components would exclude other proteins (e.g., nucleic acid binding proteins and/or proteins having enzymatic activity), for example, helicases, topoisomerases, ligases, exonucleases, endonucleases, restriction enzymes, nicking enzymes, recombinases, and the like.
The test reagents described herein may further comprise reagents for detecting and/or quantifying a nucleic acid amplification product. Suitable detection and quantification reagents can be selected by one of skill in the art based on the selected detection and/or quantification method. An amplification product may be detected and/or quantified by any suitable detection and/or quantification method including, for example, any detection method or quantification method described herein. Non-limiting examples of detection and/or quantification methods include molecular beacon (e.g., real-time, endpoint), lateral flow, fluorescence resonance energy transfer (FRET), fluorescence polarization (FP), surface capture, 5′ to 3′ exonuclease hydrolysis probes (e.g., TAQMAN), intercalating/binding dyes, absorbance methods (e.g., colorimetric, turbidity), electrophoresis (e.g., gel electrophoresis, capillary electrophoresis), mass spectrometry, nucleic acid sequencing, digital amplification, a primer extension method (e.g., iPLEX™), Molecular Inversion Probe (MIP) technology from Affymetrix, restriction fragment length polymorphism (RFLP analysis), allele specific oligonucleotide (ASO) analysis, methylation-specific PCR (MSPCR), pyrosequencing analysis, acycloprime analysis, Reverse dot blot, GeneChip microarrays, Dynamic allele-specific hybridization (DASH), Peptide nucleic acid (PNA) and locked nucleic acids (LNA) probes, AlphaScreen, SNPstream, genetic bit analysis (GBA), Multiplex minisequencing, SNAPshot, GOOD assay, Microarray miniseq, arrayed primer extension (APEX), Microarray primer extension, Tag arrays, Coded microspheres, Template-directed incorporation (TDI), colorimetric oligonucleotide ligation assay (OLA), sequence-coded OLA, microarray ligation, ligase chain reaction, padlock probes, invader assay, hybridization using at least one probe, hybridization using at least one fluorescently labeled probe, cloning and sequencing, the use of hybridization probes and quantitative real time polymerase chain reaction (QRT-PCR), nanopore sequencing, chips and combinations thereof. In some embodiments, detecting a nucleic acid amplification product comprises use of a real-time detection method (i.e., product is detected and/or continuously monitored during an amplification process). In some embodiments, detecting a nucleic acid amplification product comprises use of an endpoint detection method (i.e., product is detected after completing or stopping an amplification process). Nucleic acid detection methods may also employ the use of labeled nucleotides incorporated directly into a target sequence or into probes containing complementary sequences to a target. Such labels may be radioactive and/or fluorescent in nature and can be resolved in any of the manners discussed herein. In some embodiments, quantification of a nucleic acid amplification product may be achieved using one or more detection methods described below. In some embodiments, the detection method can be used in conjunction with a measurement of signal intensity, and/or generation of (or reference to) a standard curve and/or look-up table for quantification of a nucleic acid amplification product.
The sample prep reagent can be in a liquid form, and can provide the aqueous solution to dilute and expose the test sample DNA or RNA into solution and provide the fluid to dissolve or re-suspend the lyophilized or dried reagents once some of the sample reagent fluid is added to the reaction chamber. The testing reagents (e.g., amplification reagents) may be dried or lyophilized or in a gel or liquid format to best suit preparation, loading, storage and transport.
In some embodiments, the sample preparation fluid and the testing reagents (e.g., amplification and detection reagents) can be loaded and scaled within the cartridge at the time of manufacture prior to use.
In the shipping configuration prior to use, the cap can have a shorter length such that its lower edge does not contact the molded latching cams on the body of the cartridge. This configuration of the shipping cap can allow it to seal the sample preparation liquid reagents within the cartridge body, but for the cap to be removable by the user to start a test.
In some embodiments, the cartridge body has alignment features 110 that align and engage with mating slots in the instrument to prevent rotation of the cartridge when it is in place in the instrument. This anti-rotation feature allows the user to easily remove the shipping cap and later fit a test cap, all in single handed operation.
The cartridge body can contain the sample preparation fluid as it includes a seal on its base at location, such that with the cap fitted, it forms a scaled container or reservoir with no fluid communication to the reaction chamber(s).
In some embodiments, the reaction chamber(s) is supplied in separated packaging and is only clipped or screwed into place onto the cartridge body just prior to starting the test.
A diagnostic test assembly or ‘cartridge’ can include a sample reservoir or chamber, at least one test reservoir or reaction chamber (also referred to herein as the amplification reservoir or chamber), and at least one seal between the sample preparation reservoir and the at least one diagnostic test reservoir to prevent fluid movement between the sample preparation reservoir and the at least one diagnostic test reservoir. In some embodiments, the sample reservoir or chamber is in the form of a cylindrical cartridge body, and the amplification reservoir or chamber is in the form of an amplification tube coupled to the cartridge body by a securing ring or clip and an elastomer seal. The elastomer component can provide a seal between the amplification tube and the molded body of the cartridge such that the contents of the amplification tube will not be influenced by environmental contamination prior to use, and cannot escape during and after use. Other coupling and sealing arrangements and configurations will be apparent to those of skill in the art in light of this disclosure, and may be used in other embodiments. In some embodiments, its shipping configuration prior to use, the sample reservoir or chamber is scaled with a transport cap, and is partially filled with a sample preparation or reagent fluid, and the reaction chamber is partially filled with testing reagents (e.g., nucleic acid amplification and associated detection probe reagents). These reagents can be in liquid, gel, dried or lyophilized form. It can be advantageous in some embodiments for the sample reagent in a liquid form to provide the aqueous solution to dilute and expose the test sample DNA or RNA into solution and provide the fluid to dissolve or re-suspend the lyophilized or dried reagents once some of the sample reagent fluid is added to the amplification tube. The amplification reagents can be dried or lyophilized or in a gel or liquid format to best suit preparation, loading, storage and transport.
The cartridge body contains a sample preparation reagent 111, typically in a liquid form, and typically added during manufacture. It is an option to supply the sample reagent 111 in separate containers of one or more parts, and add these to the cartridge prior to the test when the cap is removed. The cartridge can operate in a similar manner to that for a single reaction chamber embodiment, where the sample preparation liquid 111 forms an aqueous solution to expose and carry the DNA or RNA from the sample once it is added, and to resuspend or dissolve the testing reagents (e.g., amplification reagents) in the tubes 107, 108 at the base of the cartridge once a sub-volume (e.g., a predetermined sub-volume) of the sample dilution fluid is added by the dispense action into these tubes 107, 108.
To conduct a test, the cartridge can be inserted into an instrument port to support and start heating the sample reagent fluids 111. This heating can assist, speed up or enable the sample preparation process, including cell lysis. In some embodiments, the cartridge is supported and operated within the instrument, but for the purposes of illustration, the instrument components are not shown in the drawings of the cartridge shown in
The sample can be one of many types, and may be included into the sample preparation fluid by any suitable method, such as, for example, pipette addition or droplet addition of a fluid sample, addition of a small tissue sample or a body fluid or environmental, veterinary, food or agricultural sample. In some embodiments, the test uses nucleic acid amplification, which can be very sensitive, and thus only a small amount of sample material is required to be effective in testing. A sample or test sample can be any specimen that is isolated or obtained from a subject or part thereof. Non-limiting examples of specimens include fluid or tissue from a subject, including, without limitation, blood or a blood product (e.g., serum, plasma, or the like), umbilical cord blood, bone marrow, chorionic villi, amniotic fluid, cerebrospinal fluid, spinal fluid, lavage fluid (e.g., bronchoalveolar, gastric, peritoneal, ductal, car, arthroscopic), biopsy sample, celocentesis sample, cells (e.g., blood cells) or parts thereof (e.g., mitochondrial, nucleus, extracts, or the like), washings of female reproductive tract, urine, feces, sputum, saliva, nasal mucous, prostate fluid, lavage, semen, lymphatic fluid, bile, tears, sweat, breast milk, breast fluid, hard tissues (e.g., liver, spleen, kidney, lung, or ovary), the like or combinations thereof. The term blood encompasses whole blood, blood product or any fraction of blood, such as serum, plasma, buffy coat, or the like as conventionally defined. Blood plasma refers to the fraction of whole blood resulting from centrifugation of blood treated with anticoagulants. Blood serum refers to the watery portion of fluid remaining after a blood sample has coagulated. Fluid or tissue samples often are collected in accordance with standard protocols hospitals or clinics generally follow. For blood, an appropriate amount of peripheral blood (e.g., between 3-40 milliliters) often is collected and can be stored according to standard procedures prior to or after preparation.
Suitable samples include but are not limited to saliva, blood, serum, plasma, urine, aspirate, and biopsy samples. Thus, the term “sample” with respect to a patient encompasses blood and other liquid samples of biological origin, solid tissue samples such as a biopsy specimen or tissue cultures or cells derived therefrom and the progeny thereof. The definition also includes samples that have been manipulated in any way after their procurement, such as by treatment with reagents; washed; or enrichment for certain cell populations, such as cancer cells. The definition also includes sample that have been enriched for particular types of molecules, e.g., RNAs. The term “sample” encompasses biological samples such as a clinical sample such as blood, plasma, serum, aspirate, cerebral spinal fluid (CSF), and also includes tissue obtained by surgical resection, tissue obtained by biopsy, cells in culture, cell supernatants, cell lysates, tissue samples, organs, bone marrow, and the like. A “biological sample” includes biological fluids derived therefrom (e.g., cancerous cell, infected cell, etc.), e.g., a sample comprising RNAs that is obtained from such cells (e.g., a cell lysate or other cell extract comprising RNAs).
In some embodiments, the source of the sample is a (or is suspected of being a diseased cell, fluid, tissue, or organ. In some embodiments, the source of the sample is a normal (non-diseased) cell, fluid, tissue, or organ. In some embodiments, the source of the sample is a (or is suspected of being a pathogen-infected cell, tissue, or organ. For example, the source of a sample can be an individual who may or may not be infected—and the sample could be any biological sample (e.g., blood, saliva, biopsy, plasma, serum, bronchoalveolar lavage, sputum, a fecal sample, cerebrospinal fluid, a fine needle aspirate, a swab sample (e.g., a buccal swab, a cervical swab, a nasal swab), interstitial fluid, synovial fluid, nasal discharge, tears, buffy coat, a mucous membrane sample, an epithelial cell sample (e.g., epithelial cell scraping), etc.) collected from the individual. In some embodiments, the sample is a cell-free liquid sample. In some embodiments, the sample is a liquid sample that can comprise cells. Pathogens include viruses, fungi, helminths, protozoa, malarial parasites, Plasmodium parasites, Toxoplasma parasites, Schistosoma parasites, and the like. “Helminths” include roundworms, heartworms, and phytophagous nematodes (Nematoda), flukes (Tematoda), Acanthocephala, and tapeworms (Cestoda). Protozoan infections include infections from Giardia spp., Trichomonas spp., African trypanosomiasis, amoebic dysentery, babesiosis, balantidial dysentery, Chaga's disease, coccidiosis, malaria and toxoplasmosis. Examples of pathogens such as parasitic/protozoan pathogens include, but are not limited to: Plasmodium falciparum, Plasmodium vivax, Trypanosoma cruzi and Toxoplasma gondii. Fungal pathogens include, but are not limited to: Cryptococcus neoformans, Histoplasma capsulatum, Coccidioides immitis, Blastomyces dermatitidis, Chlamydia trachomatis, and Candida albicans. Pathogenic viruses include, e.g, immunodeficiency virus (e.g., HIV); influenza virus; dengue; West Nile virus; herpes virus; yellow fever virus; Hepatitis Virus C; Hepatitis Virus A; Hepatitis Virus B; papillomavirus; and the like. Pathogenic viruses can include DNA viruses such as: a papovavirus (e.g., human papillomavirus (HPV), polyomavirus); a hepadnavirus (e.g., Hepatitis B Virus (HBV)); a herpesvirus (e.g., herpes simplex virus (HSV), varicella zoster virus (VZV), epstein-barr virus (EBV), cytomegalovirus (CMV), herpes lymphotropic virus, Pityriasis Rosea, kaposi's sarcoma-associated herpesvirus); an adenovirus (e.g., atadenovirus, aviadenovirus, ichtadenovirus, mastadenovirus, siadenovirus); a poxvirus (e.g., smallpox, vaccinia virus, cowpox virus, monkeypox virus, orf virus, pseudocowpox, bovine papular stomatitis virus; tanapox virus, yaba monkey tumor virus; molluscum contagiosum virus (MCV)); a parvovirus (e.g., adeno-associated virus (AAV), Parvovirus B19, human bocavirus, bufavirus, human parv4 G1); Geminiviridae; Nanoviridae; Phycodnaviridae; and the like. Pathogens can include, e.g., DNAviruses [e.g.: a papovavirus (e.g., human papillomavirus (HPV), polyomavirus); a hepadnavirus (e.g., Hepatitis B Virus (HBV)); a herpesvirus (e.g., herpes simplex virus (HSV), varicella zoster virus (VZV), epstein-barr virus (EBV), cytomegalovirus (CMV), herpes lymphotropic virus, Pityriasis Rosea, kaposi's sarcoma-associated herpesvirus); an adenovirus (e.g., atadenovirus, aviadenovirus, ichtadenovirus, mastadenovirus, siadenovirus); a poxvirus (e.g., smallpox, vaccinia virus, cowpox virus, monkeypox virus, orf virus, pseudocowpox, bovine papular stomatitis virus; tanapox virus, yaba monkey tumor virus; molluscum contagiosum virus (MCV)); a parvovirus (e.g., adeno-associated virus (AAV), Parvovirus B19, human bocavirus, bufavirus, human parv4 G1); Geminiviridae; Nanoviridae; Phycodnaviridae; and the like], Mycobacterium tuberculosis, Streptococcus agalactiae, methicillin-resistant Staphylococcus aureus, Legionella pneumophila, Streptococcus pyogenes, Escherichia coli, Neisseria gonorrhoeae, Neisseria meningitidis, Pneumococcus, Cryptococcus neoformans, Histoplasma capsulatum, Hemophilus influenzae B, Treponema pallidum, Lyme disease spirochetes, Pseudomonas aeruginosa, Mycobacterium leprae, Brucella abortus, rabies virus, influenza virus, cytomegalovirus, herpes simplex virus I, herpes simplex virus II, human serum parvo-like virus, respiratory syncytial virus, varicella-zoster virus, hepatitis B virus, hepatitis C virus, measles virus, adenovirus, human T-cell leukemia viruses, Epstein-Barr virus, murine leukemia virus, mumps virus, vesicular stomatitis virus, Sindbis virus, lymphocytic choriomeningitis virus, wart virus, blue tongue virus, Sendai virus, feline leukemia virus, Reovirus, polio virus, simian virus 40, mouse mammary tumor virus, dengue virus, rubella virus, West Nile virus, Plasmodium falciparum, Plasmodium vivax, Toxoplasma gondii, Trypanosoma rangeli, Trypanosoma cruzi, Trypanosoma rhodesiense, Trypanosoma brucei, Schistosoma mansoni, Schistosoma japonicum, Babesia bovis, Eimeria tenella, Onchocerca volvulus, Leishmania tropica, Mycobacterium tuberculosis, Trichinella spiralis, Theileria parva, Taenia hydatigena, Taenia ovis, Taenia saginata, Echinococcus granulosus, Mesocestoides corti, Mycoplasma arthritidis, M. hyorhinis, M. orale, M. arginini, Acholeplasma laidlawii, M. salivarium and M. pneumoniae. Pathogens can comprise one or more of SARS-COV-2, Influenza A, Influenza B, and/or Influenza C.
In some embodiments, a sample collection swab is introduced into the open cartridge. In the case of a sample collection swab being used, it is introduced into the sample chamber by a user and washed in the sample preparation fluid. The sample preparation fluid can be configured to wash the sample material from the swab, and may contain salts, dilution fluid or detergents that separate cells and cause the lysis of cell walls to, for example, expose nucleic acid components of the sample material, including DNA or RNA target material, into the sample chamber solution so that it will be suitable for subsequent nucleic acid amplification.
Other sample collection methods or sample types can be applied to the test cartridge as alternatives to a swab. These sample collection and sample addition methods may include but are not limited to: (i) use of pipette and add a sample fluid; (ii) use of a whole blood droplet directly from a finger prick; and (iii) use of an absorbent pad or membrane to collect a fluid sample such as whole blood and add it to the sample conditioning wash fluid.
Following addition of the sample, an instrument display, under control of the instrument software, can prompt the user to wait for a period of time to allow the sample preparation and cell lysis process to have sufficient time to be effective. Cell lysis procedures and reagents are known in the art and may generally be performed by chemical (e.g., detergent, hypotonic solutions, enzymatic procedures, and the like, or combination thereof), physical (e.g., French press, sonication, and the like), or electrolytic lysis methods. Any suitable lysis procedure can be utilized. For example, chemical methods generally employ lysing agents to disrupt cells and extract nucleic acids from the cells, followed by treatment with chaotropic salts. In some embodiments, cell lysis comprises use of detergents (e.g., ionic, nonionic, anionic, zwitterionic). In some embodiments, cell lysis comprises use of ionic detergents (e.g., sodium dodecyl sulfate (SDS), sodium lauryl sulfate (SLS), deoxycholate, cholate, sarkosyl).
Once the sample preparation time period has completed, the user can insert the dispensing cap assembly.
A non-limiting exemplary dispense assembly is shown in an exploded assembly view in
The piercing tips 307, 308 can comprise a geometry configured to assist fluid flow past the points 307, 308 during perforation. In the described embodiments, the piercing tips 307, 308 can comprise a geometry configured to generate a large opening in the at least one seal. The large opening can comprise a puncture of at least about 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59%, 60%, 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69%, 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 100%, or a number or a range between any two of these values, of the surface area of the at least one seal.
When the dispensing chamber 122 is assembled onto the dispense rod 121, the piercing tips 307, 308 of the dispense rod projections 305, 306 project into the cylindrical bores of the dispensing chamber 122 but do not fill its volume. These bores are shown in cross section in
In some embodiments, at this point, the piercing tips 307, 308 start to perforate the thin material section at the base of the cartridge body, and the piston or syringe features 303, 304 with “O”-ring seals progress to seal the tops of the two cylindrical bores 301, 302.
In some embodiments, with further travel, as the screw cap 120 is further closed by the user, the projection 310 deflects or breaks away to allow the O-rings 303 and 304 on the projections 305 and 306 to seal the tops of the dispense barrels, forming closed volumes of fluid within the respective dispense bores 301, 302. In some embodiments, as the user completes the cap closing action, the O-ring scaled pistons are forced by the action of the engaged threaded cap 120 and the dispensing chamber 122 being pressed into the cartridge body 101 to travel the full distance through the two dispensing insert bores 301, 302 to dispense the trapped sample fluid into each of the two amplification or test reservoirs 103.
In some embodiments, the cartridge body 101 will have around 1 to 3 milliliters of sample and sample dilution fluid 111 present, and the dispense action will dispense a small amount of this fluid, in the order of around 50 to 100 microliters, into each of the reaction chambers 103. Without change to the form of this embodiment, the scale of the parts used can be varied to vary the both the sample dilution volume and the volumes dispensed into each of the reaction chambers 103.
There is provided, in some embodiments, a dispense assembly with push-on cap (e.g., snap-fit cap). In some embodiments, the dispense cap is pushed on (e.g., a user applies force down) and snaps into place (on the cartridge body).
In some embodiments, the dispensing cap assembly is supplied fully assembled in a protective packet, and is removed and inserted by the user, but this need not be the case in other embodiments. For example, in some embodiments the sample dispensing mechanism can be attached to the removed transport cap by the user in order to form the cap assembly, and in some other embodiments the dispensing mechanism can be disposed within the sample preparation reservoir, and a cap (either the removed transport cap or a different cap) coupled to the dispensing mechanism by the act of applying the cap to the sample preparation reservoir. In some embodiments, the sample dispensing mechanism is operable to rupture or otherwise disrupt or open the at least one seal to allow sample fluid to enter the at least one diagnostic test reservoir from the sample preparation reservoir, and to dispense a predetermined sub-volume of the sample fluid from the sample preparation reservoir into the at least one diagnostic test reservoir for diagnostic testing and detection therein while preventing further fluid movement between the sample preparation reservoir and the at least one diagnostic test reservoir (e.g., reaction chamber).
In some embodiments, the dispense insert (e.g., dispensing chamber) is mounted to one end of the dispense rod. In some embodiments, the dispense rod incorporates a flange that constitutes a plunger or piston once it is inserted into a cylindrical bore or ‘cylinder’ of the dispense insert. In some embodiments, the piston forms a sliding seal by close fit with the cylindrical bore, but in other embodiments incorporates an elastomer seal to improve the seal. In some embodiments, an “0” ring is used to improve the seal for the piston as it slides within the cylindrical bore of the dispense insert.
In some embodiments, the dispense insert (e.g., dispensing chamber) incorporates openings in the form of slots in its upper section. In some embodiments, these slots are arranged such that, in the initial configuration of the dispense rod piston as the assembly is inserted into the cartridge, the internal o-ring is positioned above the base of the slots, and the slots extend out to the outer diameter of the insert such that fluid can flow both past the outside of the cylindrical bore of the insert and also through its cylindrical bore as the insert is pressed further into the sample preparation reservoir. In some embodiments, this configuration prevents pressure build up, and assists mixing of the sample fluid during insertion. Other suitable forms of the openings and configurations will be apparent to those skilled in the art, such as holes or grooves, for example, to achieve this function, where the insert does not form a seal with the internal wall of the sample preparation reservoir, and thus it can easily be moved through the sample fluid retained within the sample preparation reservoir. In some embodiments, the outer diameter and form of the dispense insert allows it to be positioned and aligned centrally within the sample preparation reservoir as it is pressed in, also to easily move down into the sample preparation reservoir as it is inserted (e.g., without significant resistance from the sample fluid). In some embodiments, this allows the base of the dispense insert to be accurately aligned with a mating recess in the base of the sample preparation reservoir.
In some embodiments, the dispense rod includes a piston flange with a sealing “O” ring and also piercing tip(s) at the end of the dispense rod. In some embodiments, once the dispense assembly is sufficiently inserted, the internal threads in the cap engage with the external thread on the body of the cartridge. In some embodiments, once these threads have mutually engaged, the user is prompted to and can progressively screw the cap closed. In some embodiments, the action of screwing the cap closed provides a mechanical advantage that facilitates the travel of the dispense assembly through the sample preparation reservoir to engage the internal components, perforate the seals at the base of the sample preparation reservoir, and dispense a sub-sample volume of sample fluid from the sample preparation reservoir into the diagnostic test reservoir.
In some embodiments, the dispense insert (e.g., dispensing chamber), after it has made contact with the base of the cartridge body, is retained on the dispense rod in such a way that some additional force is required before the dispense rod can move further into the bore of the dispense insert. In some embodiments, this additional force allows the base of the dispense insert to be pressed under friction or snapped into place into a mating or surface feature in the recess at the base of the sample preparation reservoir, forming a fluid seal therewith. In some embodiments, a small elastomer seal is included either on the dispense insert or the sample tube to assist the formation of this seal. However, in some embodiments, the injection molded form of the base of the dispense insert and the mating feature in the sample preparation reservoir are sufficient to form a fluid seal under the compression force applied as these parts come into mutual contact. In some embodiments, there is a detent formed by a circular groove in the dispense rod and a corresponding annular ring on the dispense insert. In some embodiments, this detent provides the initial break-away force to lock and seal the dispense insert into place in the base of the sample preparation reservoir prior to the completion of the dispense operation once the detent resistance is overcome and the dispense rod starts to travel through the dispense insert under the continued rotational action of the screw cap. Other arrangements for providing the dispense insert sealing force are available, and will be apparent to those of skill in the art in light of this disclosure.
In some embodiments, insert is fitted onto the dispense rod with a collapsible or crushable spacer component captured between both the dispense insert and an engagement feature extending from the dispense rod. In some embodiments, as the dispensing cap assembly is pressed into the cartridge by the screw cap action, the dispense insert makes contact with the base of the cartridge sample chamber. In some embodiments, the collapsible spacer allows the screw action to apply a force to engage the sealing action wherein the base of the cylindrical bore of the dispense insert is pressed into the mating feature in the base of the cartridge. In some embodiments, as the cap screw action applies additional force and travel, the spacer is configured to collapse in a controlled manner to press the dispense insert into place and then allow the “0” ring plunger on the dispense rod to enter the tubular bore section of the dispense mechanism. In some embodiments, after the dispense insert is held or locked in place, the “0” ring plunger on the dispense rod is caused to enter the tubular section of the dispense mechanism and form a piston and cylinder or syringe. In some embodiments, as the “0” ring plunger traps a fluid volume into the dispense tube, the piercing tip of the dispensing rod perforates the plastic section in the cartridge at the base of the tube in the dispense insert. In some embodiments, this perforation action punches a hole through the plastic section and also through the foil or plastic membrane over the top of the amplification tube. In some embodiments, continued travel of the plunger then dispenses the trapped fluid volume into the amplification tube. In some embodiments, the fluid trapped in this cylindrical section is a fixed and predetermined volume of the sample fluid that is dispensed through the perforation in the base of the sample chamber into the amplification tube mounted below.
In some embodiments, as the dispensing chamber (e.g., dispense insert) is inserted into the cartridge body 1, the sample fluid flows around it and through the open ended cylindrical dispensing bore, as shown in
In some embodiments, the dispensing chamber is initially configured so that, as the sample dispensing mechanism is inserted into the cartridge body, the sample fluid is forced to flow around the outside of the dispensing chamber before it can flow into the dispensing chamber, wherein the fluid that flows around the outside of the dispensing chamber is caused to flow through a filter or porous filler material that retains and/or traps particles and debris and/or incorporates biological or chemical components that bind to or capture components of the sample fluid that may otherwise inhibit or interfere with the sample testing.
In some embodiments, the filter components 322 physically capture and contain particles or material that would otherwise contaminate the sample fluid to be dispensed into the test reservoir. The filter components 322 can also incorporate biological or chemical components that bind to or capture components of the sample fluid that may otherwise inhibit or interfere with the test or amplification process. In some embodiments, fluid that has passed the filter components 322 will then fill the central cylindrical dispensing bore 320 from the top through the disperser slots as the part is submerged in the sample fluid. This filtered sample fluid can then be available within the dispenser bore 320 for subsequent sealing and dispensing through perforations into the attached test tube.
In some embodiments, the cartridge body comprises one or more magnetic particles with the sample preparation fluid, the surface of the magnetic particles being coated or functionalized to bind with and capture at least one predetermined target species of the biological or environmental sample when the magnetic particles are mixed within the sample fluid, and the sample dispensing mechanism is configured so that, as the sample dispensing mechanism is inserted into the cartridge body, the sample fluid is forced to flow through the dispensing chamber, and one or more magnets are located in close proximity to the inside surface of the dispensing chamber so that magnetic particles contained within the sample fluid and have captured target species are attracted to and held against the internal surface of the dispensing chamber, such that the plunger mechanism that forms a sliding seal with the internal surface of the dispensing chamber collects the magnetic particles held against the internal surface and dispenses them into the at least one reaction chamber to provide an increased concentration of the at least one predetermined target species in the predetermined sub-volume of the sample fluid dispensed into the at least one reaction chamber.
In some embodiments, the dispense insert or dispensing chamber is configured to have a sliding seal with the inside surface of the cartridge where a solid section of the insert 322 blocks fluid flowing past the outside of the central cylinder, such that all of the fluid in the sample chamber is forced to flow through the central cylindrical bore 320. Flow lines in
Once the dispense component has been pressed into a seal at the base of the sample chamber, and the piston components engage with and seal the top of the chamber, the perforation component breaks through the thin material at the base of the sample chamber 1. During the dispense process, the magnetic beads magnetically held against the inside walls of the cylinder are wiped down the bore 320 by the O-ring scaled plunger, and are thus mixed back into the sample fluid trapped within the dispense cylinder and all of this fluid and magnetic beads are dispensed into the coupled test tube by the progressive travel of the piston into the reaction chamber. This concentrates the DNA or RNA material within the sample fluid, and delivers it into the reaction chambers 107, 108. This has the advantage of concentrating and purifying the DNA or RNA nucleic acid material extracted from the sample resulting in a more sensitive and more reliable diagnostic test. The reagents within the test tube 107, 108, once eluted by the added sample fluid, can react with the molecules selectively bound to the magnetic particles. The test tube reagents can contain salts, or chemicals or a pH suitable for release of the captured material from the surface of the magnetic particles within the reaction chamber(s) 107, 108 to assist with reaction and detection of these components.
In some applications, the cartridge is used manually without an instrument. For example, in some embodiments, the cartridge is held in one hand, and the first cap removed with the other hand, the sample added and the second (dispensing) cap fitted and screwed shut. In some such embodiments, where the reaction chamber(s) are visually transparent, the dispensing of fluid into the reaction chamber(s) can be visually observed, and a color or turbidity change observed over time to provide a diagnostic test readout or display. This approach uses the advantages of operating with a fully scaled cartridge once the sample is added and internally dispensing a measured volume of diluted, prepared sample fluid into the test tube without the use of external fluid transfer steps.
Optionally, a simple stand may be provided to support the cartridge for the purpose of removing the first cap, adding the sample, and fitting and closing the dispensing cap and its associated mechanism.
Optionally, a heater block may be provided to provide temperature control of the sample and test tube chambers of the cartridge assembly, but the cartridge is manually withdrawn to observe the test result visible in one or more coupled reaction chamber(s).
In some embodiments provided herein, the cartridge can be operated within a testing apparatus or “instrument” to conduct a test (e.g., diagnostic test). The sample testing systems disclosed herein can include a testing apparatus/instrument. Details of the cartridge and the testing instrument in accordance with some embodiments of the compositions, systems, and methods provided herein are described below. By pre-loading sample preparation reagents and testing reagents into the cartridge, the sample testing system can be configured to run a specific predetermined set of one or more tests (e.g., diagnostic tests), and provide at least one indication of the test outcome(s) to a user. Different versions of the cartridge with the same physical configuration but different loaded reagents can be produced to cover a wide range of test types and diagnostic applications. In some embodiments, the instrument can automatically determine the type of diagnostic test to be performed from an identifier of the cartridge (visual or otherwise), perform the determined diagnostic test(s) and, at the completion of the diagnostic test(s), provide the diagnostic test result(s) to the user by displaying it/them on the user interface display, and/or providing it/them in the form of one or more electronic records or other form of electronic data via any of a number of communications interfaces of the instrument.
There are provided, in some embodiments, testing apparatuses. A testing apparatus can comprise a receiving port configured to receive a sample testing system provided herein. The testing apparatus can be configured to perform a test on the biological or environmental sample therein. In some embodiments, the testing apparatus further comprises a lever means configured to apply a downward force to the sample testing system placed in the receiving port. The single action can be a downward force applied to the closure relative to the cartridge body via the lever means. The downward force can form a snap fit between the closure and the cartridge body. There are provided, in some embodiments, instruments (e.g., testing apparatus) with a lever means (e.g., hinged lid). A hinged lid on the instrument can allow a user to push on the closure (e.g., dispense cap) with less force due to mechanical advantage. In some embodiments, the lid is stored vertically in a sleeve. In some embodiments, during operation, a user lifts up the hinged lid and pivots it into a horizontal position.
The testing apparatus can comprise one or more mating slots configured to align and engage with one or more alignment features of a cartridge body. The one or more mating slots can be situated in the receiving port. The one or more alignment features can prevent rotation of the cartridge body when it is in place in the testing apparatus. The one or more alignment features can enable a user to remove the second closure and/or perform the single action in a single-handed operation.
To assist with closure removal, warm-up, sample addition, sample preparation, sample dispensing, cartridge closure and test result measurement, the cartridge can supported by, aligned with, heated by and/or measured by the sample testing apparatus/instrument. In some embodiments, the instrument includes separate heater regions for independent temperature control of sample preparation and reaction chambers within the cartridge. In some embodiments of a test sequence, the cartridge with contained sample preparation fluid is inserted into the instrument, and the instrument detects the presence of the cartridge and begins warming the sample preparation fluid. When the sample preparation fluid has reached the desired temperature, the instrument can then prompt the user to add the biological or environmental sample to be analyzed. The heating of the sample preparation fluid can be useful to assist with rapid and efficient sample preparation.
Subsequently, or when prompted by the instrument, the user can then apply a closure to the cartridge body, and the action of operating the closure can not only seal the sample and sample preparation fluid within the cartridge, but also actuate a dispensing mechanism within the cartridge body to deliver a sub-sample of predetermined volume into one or more reaction chambers within the cartridge.
In some embodiments, the instrument then controls the temperature of the one or more reaction chambers and the sample fluid and testing reagents contained within them. This temperature control can be to maintain a fixed temperature, or to follow a predetermined time varying temperature profile, for example, or in the case of a PCR reaction, subjected to thermal cycling with heating and cooling between different fixed temperatures. In any case, a cycle series or a time series of optical measurements of the contents of the reaction chamber(s) can be acquired by the instrument. The instrument can process these measurements to determine a test result which can then be displayed or otherwise provided as an output to a user.
Provided herein are compositions, systems, and methods comprising and/or employing a testing instrument or apparatus which includes one or more of the following: (i) an instrument housing with an access port to accept a plastic cartridge assembly; (ii) a sensor or switch to detect the insertion or presence of the cartridge inserted into the apparatus; (iii) controller electronics and associated internal electronics, microprocessor and memory to run a software program and save data for future recall and use; (iv) electrical interface connectors for connection of USB, serial or Ethernet connected peripherals interfaces and external memory devices; (v) embedded software to provide functions to sequence processing of the instrument, the cartridge and acquire diagnostic test measurements for interpretation determination of test outcome; (vi) a temperature controlled sample chamber heater block to provide heating and temperature control of the upper sample chamber section of the cartridge assembly; (vii) a temperature controlled heater block to provide heating and temperature control of the upper contact specific reaction chamber(s) (e.g., amplification test wells) in an inserted cartridge where this block can apply controlled temperatures including temperature cycling to fluids within the cartridge wells; and/or (viii) sensors to detect and provide measurement of optical absorption, fluorescence or bioluminescence characteristics of the reagents and added sample fluid reactions within the reaction chamber(s) during the course of the test running and at the completion of the test.
The instrument apparatus can incorporate one or more optical sensors where these sensors can be scanned along a row of test wells to allow a multitude of measurements to be recorded for each test well using one or more different sensors. In some embodiments, the instrument controller may be located remotely from the physical body of the apparatus such as on a remote server, and manage and control the operation of the apparatus over a communication network such as the internet. In some embodiments, one or more of the sensors is a coaxial fluorescence sensor where optically filtered emissions from a light emitting diode, or laser illumination of a selective wavelength range is emitted from the sensor lens. In some embodiments, this illumination causes optical excitation of the sample in the test well and the same lens also captures florescence emission from the sample at a different shifted wavelength. In some embodiments, this sample fluorescence emission is measured and forms a measurement used in determining the diagnostic test result. In some embodiments, one or more of the sensors can detect fluorescence within the sample contained within each test well using a separated excitation illumination source to optically excite the test sample and a separated sensor to measure the resulting fluorescence emission. In some embodiments, one or more of the sensors uses reflectance or transmission of specific optical illumination wavelength ranges to measure optical reflectance or absorption within the test sample contained within each test well. In some embodiments, one or more of the sensors measures light emission from the test sample, where this emission is caused by luminescence or bio-luminescence within the test sample. In some embodiments, the sensors are scanned at constant speed past all of the wells, and a multitude of measurements acquired. Subsequent processing of this data set of measurements can determine the measurement values to assign to each test well. This analysis can consider such characteristics as the relative position or the acquisition time of each measurement and local peaks with an interpolated curve encompassing the acquired measurements.
In some embodiments, the instrument apparatus incorporates one or more ultraviolet light sources, where this ultraviolet illumination can be turned on or off by the instrument controller. In some embodiments, the instrument apparatus incorporates one or more reference targets within the field of view of the fluorescence or optical absorption sensors. In some embodiments, the test apparatus includes at least one sensing component configured to determine a degree of rotation and/or thread progression of the closure, and the test apparatus is configured to prompt a user to complete the closure operation if the at least one sensing component has determined that the closure operation is incomplete; and to automatically progress to a next stage of diagnostic testing if the closure operation has been determined as being complete.
In some embodiments, at least one of the at least one reaction chambers is transparent, and the test apparatus is configured to determine a test result in the at least one reaction chamber by detecting or measuring a change in emission and/or absorption at one or more wavelengths within the at least one reaction chamber, wherein the test apparatus is optionally configured to illuminate the at least one reaction chamber to enhance or produce the detecting or measuring. In some embodiments, the test apparatus and the sample testing system (e.g., diagnostic test assembly) include respective alignment and support features configured for mutual engagement to ensure that the sample testing system is received in a predetermined alignment with respect to the test apparatus and to maintain the alignment when the closure is applied to the cartridge body after receipt of the biological or environmental sample and the sample dispensing mechanism therein.
The test apparatus can include one or more components configured to apply a changing and/or moving magnetic field to the sample testing system to cause corresponding movements of magnetic particles within at least one of the cartridge body and the at least one reaction chamber, and thereby cause mixing of the sample and sample preparation fluid therein.
In some embodiments, the test apparatus and the sample testing system are configured to allow the test apparatus to independently control the temperatures of the cartridge body and the at least one reaction chamber.
In some embodiments, the test apparatus includes one or more image sensors configured to generate image data representing one or more images of at least a portion of the sample testing system, wherein the images represent at least one of: (i) fluid distribution within at least one of the at least one reaction chamber and the cartridge body, and the test apparatus is configured to process the image data to monitor dispensing of the sample fluid, and to proceed to a next stage of diagnostic testing if the monitoring has determined that the dispensing is complete; and (ii) a fluid volume contained within the at least one reaction chamber, and the test apparatus is configured to process the image data to allow compensation for the volume tolerances in the dispensed fluid to allow for improved test result determination. In some embodiments, the test apparatus includes one or more optical sensors mounted to a translation stage under control of a controller of the test apparatus so that the optical sensors can measure optical absorption or emission or fluorescence from one or more selected reaction chambers of the sample testing system.
In some embodiments, the test apparatus includes at least one ultra violet (UV) emission source to denature samples contained within the sample testing system following a diagnostic test to inhibit contamination in the event of sample fluid escaping from the sample testing system.
One or more image sensors can incorporated within the instrument can capture digital images of the cartridge and the progression of the dispensing mechanism components and the state and progress of the fluids contained within the cartridge. The image data acquired by the image sensor and in subsequent image analysis can be used by the controller to determine the levels of the dispensed sample fluid in each of the reaction chamber(s) 107, 108 and to use this level to determine that the sample fluid dispensing has completed correctly. The level of the fluid dispensed into each of the one or more reaction chamber(s) 107, 108 within the cartridge can be used to compensate the test result for tolerances in the dispensing operation. The level of the fluid with each test tube can be converted by the controller to a volume by using a mathematical model of the tube 107, 108 or by using a look up table. The volume of dispensed fluid can influence the concentration of the test regents within the test chamber fluid once they have dissolved into the dispensed fluid. By measuring the volume of the dispensed sample fluid, the concentration of reagents within each test tube 107, 108 can be calculated. From a series of previously conducted experiments or from a model of the test reactions, the effect of test reagent concentration on the test result and the interpretation of the time series measurements of the test to interpret the result can be known and adjusted or compensated for within the apparatus. The fluid sample preparation reagent stored in the cartridge can be colored with a dye. This dye can be used by the image sensor to visually image colored or contrasting fluid flow into the cartridge reaction chamber(s) 107, 108 to confirm the dispensing action and confirm the dispense volume. Image analysis of an image of the fluid dispensed into one or more of the coupled reaction chamber(s) 107, 108 can be used to measure the volume within the tube 107, 108, and this measurement can be used to compensate the test result calculation for the amplification volume. This compensation can be of particular significance for a quantitative test result where the concentration of reagents in the test tube 107, 108 can influence the measurements and reaction response.
Although some embodiments employ optical measurements of the reaction chamber to determine a test result, it is recognized that sensors with alternative measurement methods can operate in the same test apparatus/instrument and with the diagnostic test cartridges described herein. These sensors can use magnetic, electrical, atomic or physical properties of the test fluids to acquire measurements suitable to determine a test result.
Some embodiments of the compositions and methods provided herein contemplate mixing of the contents of either the cartridge body or the reaction chamber(s), which can, in some embodiments, improve test reliability or accuracy. To achieve mixing, magnetic inserts such as small steel or ferrite balls can be included in the cartridge body and/or the reaction chamber(s) 107, 108 during reagent loading of the cartridge (e.g., during its initial manufacture). Some embodiments contemplate applying an external magnetic field to the cartridge body (e.g., provided by a testing apparatus moving a permanent magnet or plurality of magnets into proximity) to induce mixing within the sample fluid and/or reaction chambers. Mixing within the cartridge body can be used to mix introduced sample material with the sample preparation fluid to dilute and prepare the sample material for amplification. This preparation mixing can also improve cell lysis and the extraction and preparation of the target DNA or RNA nucleic acid material within the sample.
In at least some of the previously described embodiments, one or more elements used in an embodiment can interchangeably be used in another embodiment unless such a replacement is not technically feasible. It will be appreciated by those skilled in the art that various other omissions, additions and modifications may be made to the methods and structures described above without departing from the scope of the claimed subject matter. All such modifications and changes are intended to fall within the scope of the subject matter, as defined by the appended claims.
With respect to the use of substantially any plural and/or singular terms herein, those having skill in the art can translate from the plural to the singular and/or from the singular to the plural as is appropriate to the context and/or application. The various singular/plural permutations may be expressly set forth herein for sake of clarity. As used in this specification and the appended claims, the singular forms “a,” “an,” and “the” include plural references unless the context clearly dictates otherwise. Any reference to “or” herein is intended to encompass “and/or” unless otherwise stated.
It will be understood by those within the art that, in general, terms used herein, and especially in the appended claims (e.g., bodies of the appended claims) are generally intended as “open” terms (e.g., the term “including” should be interpreted as “including but not limited to,” the term “having” should be interpreted as “having at least,” the term “includes” should be interpreted as “includes but is not limited to,” etc.). It will be further understood by those within the art that if a specific number of an introduced claim recitation is intended, such an intent will be explicitly recited in the claim, and in the absence of such recitation no such intent is present. For example, as an aid to understanding, the following appended claims may contain usage of the introductory phrases “at least one” and “one or more” to introduce claim recitations. However, the use of such phrases should not be construed to imply that the introduction of a claim recitation by the indefinite articles “a” or “an” limits any particular claim containing such introduced claim recitation to embodiments containing only one such recitation, even when the same claim includes the introductory phrases “one or more” or “at least one” and indefinite articles such as “a” or “an” (e.g., “a” and/or “an” should be interpreted to mean “at least one” or “one or more”); the same holds true for the use of definite articles used to introduce claim recitations. In addition, even if a specific number of an introduced claim recitation is explicitly recited, those skilled in the art will recognize that such recitation should be interpreted to mean at least the recited number (e.g., the bare recitation of “two recitations,” without other modifiers, means at least two recitations, or two or more recitations). Furthermore, in those instances where a convention analogous to “at least one of A, B, and C, etc.” is used, in general such a construction is intended in the sense one having skill in the art would understand the convention (e.g., “a system having at least one of A, B, and C” would include but not be limited to systems that have A alone, B alone, C alone, A and B together, A and C together, B and C together, and/or A, B, and C together, etc.). In those instances where a convention analogous to “at least one of A, B, or C, etc.” is used, in general such a construction is intended in the sense one having skill in the art would understand the convention (e.g., “a system having at least one of A, B, or C” would include but not be limited to systems that have A alone, B alone, C alone, A and B together, A and C together, B and C together, and/or A, B, and C together, etc.). It will be further understood by those within the art that virtually any disjunctive word and/or phrase presenting two or more alternative terms, whether in the description, claims, or drawings, should be understood to contemplate the possibilities of including one of the terms, either of the terms, or both terms.
In addition, where features or aspects of the disclosure are described in terms of Markush groups, those skilled in the art will recognize that the disclosure is also thereby described in terms of any individual member or subgroup of members of the Markush group.
As will be understood by one skilled in the art, for any and all purposes, such as in terms of providing a written description, all ranges disclosed herein also encompass any and all possible sub-ranges and combinations of sub-ranges thereof. Any listed range can be easily recognized as sufficiently describing and enabling the same range being broken down into at least equal halves, thirds, quarters, fifths, tenths, etc. As a non-limiting example, each range discussed herein can be readily broken down into a lower third, middle third and upper third, etc. As will also be understood by one skilled in the art all language such as “up to,” “at least,” “greater than,” “less than,” and the like include the number recited and refer to ranges which can be subsequently broken down into sub-ranges as discussed above. Finally, as will be understood by one skilled in the art, a range includes each individual member. Thus, for example, a group having 1-3 articles refers to groups having 1, 2, or 3 articles. Similarly, a group having 1-5 articles refers to groups having 1, 2, 3, 4, or 5 articles, and so forth.
While various aspects and embodiments have been disclosed herein, other aspects and embodiments will be apparent to those skilled in the art. The various aspects and embodiments disclosed herein are for purposes of illustration and are not intended to be limiting, with the true scope and spirit being indicated by the following claims.
This application is a continuation of PCT Application No. PCT/US2022/075949, filed Sep. 2, 2022, which claims priority to U.S. Provisional Application No. 63/241,033, filed Sep. 6, 2021, each of which is hereby incorporated by reference in its entirety.
| Number | Date | Country | |
|---|---|---|---|
| 63241033 | Sep 2021 | US |
| Number | Date | Country | |
|---|---|---|---|
| Parent | PCT/US2022/075949 | Sep 2022 | WO |
| Child | 18587777 | US |
