CROSS-REFERENCE TO RELATED PATENT APPLICATION
This application claims the benefit of Korean Patent Application No. 10-2007-0002643, filed on Jan. 9, 2007, in the Korean Intellectual Property Office, the disclosure of which is incorporated herein in its entirety by reference.
BACKGROUND OF THE INVENTION
1. Field of the Invention
The present invention relates to a method of predicting risk of lung cancer recurrence in a lung cancer patient or after a patient has lung cancer treatment, a method of preparing a report on the risk of lung cancer recurrence in a lung cancer patient or after a patient has lung cancer treatment, a report prepared by the same, and a composition, kit and microarray for diagnosing the risk of lung cancer recurrence in a lung cancer patient or after a patient has lung cancer treatment.
2. Description of the Related Art
Lung cancer is the leading cause of death due to cancer in the world. Lung cancer is categorized into two types, small cell lung cancer (SCLC) and non-small cell lung cancer (NSCLC), and about 80% of lung cancer cases are categorized as NSCLC. NSCLC is categorized into three sub-types: 40% of adenocarcinoma, 40% of squamous cell carcinoma and 20% of large cell carcinoma. Currently, a TMN staging system is widely accepted in the management of lung cancer.
In the TMN staging system, the primary tumor is subdivided into four T categories (T1-T4) depending upon the tumor size, site and local involvement. Lymph node spread is subcategorized into bronchio/pulmonary within the lung (N1), mediastinal spread on the same side of the lung as the primary tumor (N2) and mediastinal spread on the side of the lung opposite to the side having the primary tumor or supraclavicular involvement (N3). Distal or metastatic spread is either absent or present (M0 or M1). In general, lung cancer that does not metastasize is treated by being removed through a surgical operation. However, recurrence rate after a lung cancer removal operation is as high as 20 to 50% (Cancer: Principles & Practice of Oncology, 56th. ed. In: Devita D V, Hellman S. Rosenberg S A, eds. Philadelphia, Pa.: Lippincott Williams & Wilkins, 2001).
Conventionally, a method of diagnosing lung cancer using a marker gene specific to lung cancer is known. For example, U.S. Patent Publication No. 2006025057 discloses a method of diagnosing lung cancer using a marker specific to lung cancer. Further, U.S. Patent Publication No. 20050272061 discloses a method of diagnosing cancer in an individual, comprising measuring an L gene that is specifically and distinctively expressed in lung cancer tissues and cells, and its products.
However, there is still a need for developing a method of effectively predicting the risk of lung cancer recurrence in a lung cancer patient or a patient who has had lung cancer treatment to the extent that the method is applied to clinical practices.
SUMMARY OF THE INVENTION
The present invention provides a method of predicting risk of lung cancer recurrence in a lung cancer patient or after a patient has lung cancer treatment.
The present invention also provides a method of preparing a report on the risk of lung cancer recurrence in a lung cancer patient or after a patient has lung cancer treatment and a report prepared by the same.
The present invention also provides a composition, kit and microarray for diagnosing the risk of lung cancer recurrence in a lung cancer patient or after a patient has lung cancer treatment.
According to an aspect of the present invention, there is provided a method of predicting risk of lung cancer recurrence in a lung cancer patient or after a patient has lung cancer treatment, the method comprising:
obtaining a biological sample from a lung cancer patient;
measuring an expression level of at least one marker gene from the biological sample, the marker gene being selected from the group consisting of marker genes of Table 1, 2 or 3 to obtain data for the expression level of the marker gene; and
determining whether the expression level of the marker gene corresponds to an expression level of a recurrence group or an expression level of a non-recurrence group.
The method of predicting risk of lung cancer recurrence in a lung cancer patient or after a patient has lung cancer treatment includes obtaining a biological sample from a lung cancer patient.
The obtaining a biological sample may include any operation that obtains a sample including an arbitrary cell from a lung cancer patient. For example, the biological sample may be blood, plasma, serum, urine, tissue, cell, organ, bone marrow, saliva, expectoration, cerebrospinal fluid and the like, but is not limited thereto. The biological sample may be preferably lung cancer tissue. The biological sample may be lung cancer tissue removed during a lung cancer removal operation, but is not necessarily obtained by the lung cancer removal operation. The obtainment of the lung cancer tissue may be physically conducted or optically conducted through a laser or the like.
The method of predicting a risk of lung cancer recurrence in a lung cancer patient or a patient after a lung cancer treatment includes measuring an expression level of at least one marker gene selected from the group consisting of marker genes of Table 1, 2 or 3 in the sample to obtain data for the expression level of the marker gene.
The measuring an expression level of the marker gene may be performed by measuring an expression level of at least one marker gene selected from the group consisting of marker genes of Table 1. Preferably, in this operation, expression levels of at least 2, 4, 6, 8, 10, 15, 20, 30, 70, 100, 150 or a total of 166 marker genes selected from the group consisting of marker genes of Table 1 may be measured. In this case, the lung cancer may be adenocarcinoma or squamous cell carcinoma.
When the lung cancer is adenocarcinoma, the measuring an expression level of the marker gene may be performed by measuring an expression level of at least one marker gene selected from the group consisting of marker genes of Table 2. Preferably, in this operation, expression levels of at least 2, 4, 6, 8, 10, 15, 20, 30, 70, 100, 150, 200, 250 or a total of 300 marker genes selected from the group consisting of marker genes of Table 2 may be measured.
When the lung cancer is squamous cell carcinoma, the measuring an expression level of the marker gene may be performed by measuring an expression level of at least one marker gene selected from the group consisting of marker genes of Table 3. Preferably, in this operation, an expression level of at least 2, 4, 6, 8, 10, 15, 20, 30, 70, 100, 150, or a total of 166 marker genes selected from the group consisting of marker genes of Table 3 may be measured.
The measuring an expression level of the marker gene includes measuring an arbitrary expression product expressed from the maker gene. For example, this operation may be measuring a level of mRNA or protein derived from the marker gene.
The “measurement of a level of mRNA” may be analyzed using a conventional method including RT-PCR, competitive RT-PCR, real-time RT-PCR, RNase protection assay, northern blotting, DNA microarray and the like. Preferably, the measurement of a level of mRNA may be carried out by hybridizing mRNA isolated from the biological sample or cDNA derived therefrom on a microarray on which a probe specific to at least one marker gene selected from the group consisting of marker genes of Tables 1, 2 and 3 is immobilized to measure a degree of the obtained hybridization. The degree of the hybridization may be measured using an arbitrary measurement method known to those of ordinary skill in the art, such as fluorescence measurement and electrical measurement. In this case, the probe or target nucleic acid may be labeled with a detectable appropriate marker. Herein, the cDNA may be directly amplified by RT-PCR using sense and anti-sense primer pair targeted to at least one marker gene selected from the group consisting of marker genes of Tables 1, 2 and 3 as a primer.
The “measurement of a level of protein” may be conducted using any conventional protein measuring or detecting method. For example, the measurement of a level of protein may be conducted using an analysis method that uses an antibody that specifically binds with protein expressed from at least one marker gene selected from the group consisting of marker genes of Tables 1, 2 and 3. Examples of the protein analysis method using an antibody may include western blotting, enzyme-linked immunosorbent assay (ELISA), radioimmunoassay, radioimmunodiffusion, Ouchterlony immunodiffusion, rocket immunoelectrophoresis, immunoprecipitation assay, complement fixation analysis, Fluorescence Activated Cell Sorting (FACS) and the like, but are not limited thereto. Examples of the ELISA include a direct ELISA, an indirect ELISA, a direct sandwich ELISA, an indirect sandwich ELISA and the like. The western blotting is a method in which total protein is isolated and electrophoresized to separate protein according to their size, the separated proteins are then moved into a nitrocellulose membrane to be reacted with an antibody, and a generated amount of the antigen-antibody complex is confirmed using a labeled antibody. In addition, the level of protein may be measured using enzyme, substrate, coenzyme, ligand or the like that specifically binds with the target protein.
The expression level of the marker gene may be determined by measuring an amount of an amplification product obtained by nucleic acid amplification that is carried out by a reverse transcriptase-polymerase chain reaction (RT-PCR) using RNA isolated from the sample as a template.
In addition, the method of predicting a risk of lung cancer recurrence in a lung cancer patient or a patient after a lung cancer treatment includes determining whether the expression level of the marker gene corresponds to an expression level of a recurrence group or an expression level of a non-recurrence group.
The term “recurrence group” refers to a group of patients with lung cancer recurrence within a certain period after a lung cancer treatment among lung cancer patients. Preferably, the term “recurrence group” may refer to a group of patients with lung cancer recurrence within one year after a lung cancer removal operation among lung cancer patients. However, types of lung cancer treatment and a period which is a basis of recurrence may be appropriately adjusted by those of ordinary skill in the art. In addition, the term “non-recurrence group” refers to a group of patients without lung cancer recurrence even after a certain period passes by after a lung cancer treatment among lung cancer patients. Preferably, the term “non-recurrence group” refers to a group of patients without lung cancer recurrence even after three years after a lung cancer removal operation among lung cancer patients. However, types of lung cancer treatment and a period which is a basis of non-recurrence may be appropriately adjusted by those of ordinary skill in the art.
The “expression level of recurrence group” or “expression level of non-recurrence group” corresponds to a standard expression level. Through preliminary experiment, a biological sample of a lung cancer patient, for example, lung cancer tissue is collected in advance. An expression level of at least one marker gene selected from the group consisting of marker genes of Tables 1, 2 and 3 in the lung cancer tissue is then measured. Patients after lung cancer treatment are divided into a recurrence group and a non-recurrence group in which recurrence and non-recurrence respectively occur as time passes by. Next, each of expression levels of the marker gene measured in the recurrence and non-recurrence groups is divided into an expression level of the recurrence group or the non-recurrence group.
The determining whether the expression level of the marker gene corresponds to an expression level of a recurrence group or an expression level of a non-recurrence group may be performed using a statistical forecasting model. In this case, whether the expression level of the marker gene corresponds to an expression level of a recurrence group or an expression level of a non-recurrence group is determined by whether the expression levels show a statistically meaningful difference from each other.
Whether there is a statistically meaningful difference may be determined using a statistical analysis model known to those of ordinary skill in the art. Preferably, the statistical analysis model may be a statistical forecasting model selected from the group consisting of a Linear Discrimination Analysis (LDA) model, a Quadratic Discrimination Analysis (QDA) prediction model, a Neural Network model, a Decision Tree model, a Support Vector Machine model and a Naive Bayes model, but is not limited thereto.
Examples of the determining whether the expression level of the marker gene corresponds to an expression level of a recurrence group or an expression level of a non-recurrence group include determining to correspond to a non-recurrence group if the expression level of the marker gene shows a statistically meaningful difference from the expression level of the recurrence group, and determining to correspond to a recurrence group if the expression level of the marker gene shows a statistically meaningful difference from the expression level of the non-recurrence group. In addition, examples of the determining whether the expression level of the marker gene corresponds to an expression level of a recurrence group or an expression level of a non-recurrence group include determining to correspond to a recurrence group if the expression level of the marker gene does not show a statistically meaningful difference from the expression level of the recurrence group, and determining to correspond to a non-recurrence group if the expression level of the marker gene does not show a statistically meaningful difference from the expression level of the non-recurrence group.
The statistically meaningful difference may have p values that are statistically meaningfully higher or lower than the expression level of the recurrence group or non-recurrence group. Preferably, the p value may be less than 0.05.
In the method of predicting a risk of lung cancer recurrence in a lung cancer patient or a patient after a lung cancer treatment, if the expression level of the marker gene is determined to correspond to the expression level of the recurrence group, a risk of lung cancer recurrence in a patient can be predicted to be high. In addition, if the expression level of the marker gene is determined to correspond to the expression level of the non-recurrence group, a risk of lung cancer recurrence in a patient can be predicted to be low.
In the method of predicting a risk of lung cancer recurrence in a lung cancer patient or a patient after a lung cancer treatment, specificity may be at least 50%, preferably 60%, more preferably at least 70%, far more preferably at least 80%, and most preferably 90%.
According to another aspect of the present invention, there is provided a method of preparing a report on the risk of lung cancer recurrence in a lung cancer patient or after a patient has lung cancer treatment, the method comprising preparing a report representing predicted results according to the method of predicting risk of lung cancer recurrence in a lung cancer patient or after a patient has lung cancer treatment.
The report may include probability of recurrence according to time.
According to another aspect of the present invention, there is provided a report on a risk of lung cancer recurrence in a lung cancer patient or a patient after a lung cancer treatment, which is prepared by the method of preparing a report on the risk of lung cancer recurrence in a lung cancer patient or after a patient has lung cancer treatment.
According to another aspect of the present invention, there is provided a composition for diagnosing the risk of lung cancer recurrence in a lung cancer patient or after a patient has lung cancer treatment, comprising at least one probe or probe set selected from marker genes selected from the group consisting of marker genes of Tables 1, 2 and 3.
The composition may further comprise a reagent required for hybridization reaction with the marker gene in a sample or nucleic acid products expressed therefrom. In addition, the composition may further comprise a buffer, a solvent or the like that stabilizes the probe and acts as a medium of the reaction.
The term “probe” used through the present application refers to a nucleic acid strand that is partially or completely complementary to a target nucleic acid, and refers to oligonucleotide that can bind with the target nucleic acid by a base-specific method. Preferably, the probe may be oligonucleotide that is completely complementary to the target nucleic acid. The probe can be a conventionally known arbitrary nucleic acid derivative that can complementarily bind to the target nucleic acid, such as peptide nucleic acid as well as nucleic acid.
The binding of the probe with the target nucleic acid (in general, referred to as hybridization) may be sequence-dependently carried out under various conditions. In general, the hybridization is performed in a specific ion intensity at specific pH at a temperature that is about 5° C. lower than Tm with respect to a specific sequence. The Tm refers to a state at which 50% of probe complementary to a target sequence is bound to the target sequence. Examples of the conditions of the hybridization may include a pH in the range of 7.0-8.3 and a Na+ ion concentration of 0.01-1.0 M. In addition, to raise specificities of the target nucleic acid and the probe, the hybridization may be carried out under conditions that make the binding of the probe with the target nucleic acid unstable, for example, at a high temperature and in the presence of a high concentration of an unstabilizing agent (for example formamide).
The probe may be any length of polynucleotide that can sequence-specifically be bound to the target nucleic acid. For example, the length of the probe may be 7-200 nucleotides, 7-150 nucleotides, 7-100 nucleotides, 7-50 nucleotides, or a full-length strand of gene, but is not limited thereto.
The probe may be labeled with a detectable marker. The detectable marker may be a fluorescent marker such as Cy3 or Cy5, a radioactive material marker, enzyme that converts a substrate to chromogen, or the like, but is not limited thereto.
According to another aspect of the present invention, there is provided a kit for diagnosing the risk of lung cancer recurrence in a lung cancer patient or after a patient has lung cancer treatment, comprising at least one probe or probe set selected from marker genes selected from the group consisting of marker genes of Tables 1, 2 and 3.
The probe is the same as defined above. The probe may be labeled with a detectable marker. The detectable marker may be a fluorescent marker such as Cy3 or Cy5, a radioactive material marker, enzyme that converts a substrate to chromogen, or the like, but is not limited thereto.
In the kit, the probe or probe set may be immobilized on a microarray. A target nucleic acid in a sample is hybridized with the probe on the microarray, and the presence and concentration of the target nucleic acid may be determined by measuring the hybridized results. During the hybridization, the target nucleic acid may be labeled with a detectable marker.
The kit may further include a manual that describes a process of measuring a risk of lung cancer recurrence in a lung cancer patient or a patient after a lung cancer treatment.
According to another aspect of the present invention, there is provided a kit for diagnosing the risk of lung cancer recurrence in a lung cancer patient or after a patient has lung cancer treatment, comprising sense and anti-sense primer pair with respect to at least one marker gene selected from the group consisting of marker genes of Tables 1, 2 and 3.
The term “primer” used herein refers to a nucleic acid having a free 3′ hydroxy group that is partially or completely complementary to a target nucleic acid and can bind with the template nucleic acid by a sequence-specific method, and refers to oligonucleotide that functions as a starting point for template strand transcription in polymerization.
The kit may further comprise a reagent required for PCR or RT-PCR using the primer described above as a primer and the target nucleic acid as a template. The reagent may include a buffer solution, a DNA polymerase (and/or reverse transcriptase), and 4 types of dNTPs.
The primer may be any length of polynucleotide that can sequence-specifically be bound to the template nucleic acid and function as a starting point for template strand transcription in polymerization. For example, the length of the primer may be 7-200 nucleotides, 7-150 nucleotides, 7-100 nucleotides, 7-50 nucleotides, or a full-length strand of a gene, but is not limited thereto.
The primer may be labeled with a detectable marker. The detectable marker may be a fluorescent marker such as Cy3 or Cy5, a radioactive material marker, enzyme that converts a substrate to chromogen, or the like, but is not limited thereto.
According to another aspect of the present invention, there is provided a microarray for diagnosing a risk of lung cancer recurrence in a lung cancer patient or a patient after a lung cancer treatment, in which at least one probe or probe set selected from marker genes selected from the group consisting of marker genes of Tables 1, 2 and 3.
The term “microarray” refers to a polynucleotide group immobilized on a substrate in a high concentration. The polynucleotide group is respectively immobilized on a certain region. Such microarray is well-known to those of ordinary skill in the art. The microarray is, for example, disclosed in U.S. Pat. Nos. 5,445,934 and 5,744,305, and contents of these patents are included in the present application by reference. The substrate may have various shapes such as plate, film and microsphere (or bead).
The probe is the same as defined above. The probe may be labeled with a detectable marker. The detectable marker may be a fluorescent marker such as Cy3 or Cy5, a radioactive material marker, enzyme that converts a substrate to chromogen, or the like, but is not limited thereto.
The gene expression pattern of the lung cancer cell after lung cancer tissue removal operation is analyzed through a hybridization with the probe on the microarray, and a marker gene that is determined to have a difference in an expression level between a patient with lung cancer recurrence within one year (recurrence group) and a patient without lung cancer recurrence even after three years (non-recurrence group) is selected. The results are shown in Table 1 below. A total number of patients was 60. Among them, the number of patients with lung cancer recurrence within one year after lung cancer tissue removal operation was 19, and the number of patients without lung cancer recurrent even after three years was 41.
TABLE 1
|
|
Genbank
T-test
Fold change
|
NO.
Probe Set ID
Gene Name
Gene Symbol
Accession #
p-value
(abs)
|
|
|
001
1552486_s_at
lactamase, beta
LACTB
NM_171846
0.005162234
1.522293
|
002
1553105_s_at
desmoglein 2
DSG2
NM_001943
0.019467462
2.3323212
|
003
1553530_a_at
integrin, beta 1 (fibronectin receptor, beta polypeptide,
ITGB1
NM_033669
0.01684671
1.7791877
|
antigen CD29 includes MDF2 MSK12)
|
004
1553678_a_at
integrin, beta 1 (fibronectin receptor, beta polypeptide,
ITGB1
NM_133376
0.012459265
1.7374801
|
antigen CD29 includes MDF2, MSK12)
|
005
1554087_at
hypothetical protein FLJ32549
FLJ32549
BC036246
0.002290308
1.5143739
|
006
1554761_a_at
hypothetical protein FLJ20397
FLJ20397
BC010850
0.001210456
1.6267678
|
007
1555326_a_at
ADAM metallopeptidase domain 9 (meltrin gamma)
ADAM9
AF495383
0.012324799
2.1980886
|
008
1555564_a_at
I factor (complement)
IF
BC020718
0.007528743
2.5875902
|
009
1555705_a_at
chemokine-like factor superfamily 3
CKLFSF3
AY168714
0.004961676
1.8587251
|
010
1557987_at
PI-3-kinase-related kinase SMG-1-like locus
LOC641298
BC042832
0.010989661
1.7944587
|
011
1558678_s_at
metastasis associated lung adenocarcinoma transcript 1
MALAT1
BE708432
0.00670648
1.6990829
|
(non-coding RNA)
|
012
160020_at
matrix metallopeptidase 14 (membrane-inserted)
MMP14
Z48481
0.005463324
1.5193439
|
013
200604_s_at
protein kinase, cAMP-dependent, regulatory,
PRKAR1A
M18468
0.017312625
1.5803499
|
type I, alpha (tissue specific extinguisher 1)
|
014
200615_s_at
adaptor-related protein complex 2, beta 1 subunit
AP2B1
AL567295
0.007407852
1.6839108
|
015
200864_s_at
RAB11A, member RAS oncogene family
RAB11A
NM_004663
0.000163535
1.5653288
|
016
200922_at
KDEL (Lys-Asp-Glu-Leu) endoplasmic reticulum
KDELR1
NM_006801
0.004791257
1.638207
|
protein retention receptor 1
|
017
201020_at
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase
YWHAH
NM_003405
0.009279575
1.5148095
|
activation protein, eta polypeptide
|
018
201179_s_at
guanine nucleotide binding protein (G protein),
GNAI3
J03005
0.014834337
1.5069977
|
alpha inhibiting activity polypeptide 3
|
019
201309_x_at
chromosome 5 open reading frame 13
C5orf13
U36189
0.011555359
2.1326842
|
020
201363_s_at
influenza virus NS1A binding protein
IVNS1ABP
AB020657
0.00119686
1.5838884
|
021
201505_at
laminin, beta 1
LAMB1
NM_002291
0.000568398
1.8073287
|
022
201506_at
transforming growth factor, beta-induced, 68 kDa
TGFBI
NM_000358
0.008768089
1.9059453
|
023
201548_s_at
Jumonji, AT rich interactive domain 1B (RBP2-like)
JARID1B
W02593
0.010550437
1.5276276
|
024
201559_s_at
chloride intracellular channel 4
CLIC4
AF109196
0.002245945
2.1570368
|
025
201564_s_at
fascin homolog 1, actin-bundling protein
FSCN1
NM_003088
0.007795681
2.1724482
|
(Strongylocentrotus purpuratus)
|
026
201578_at
podocalyxin-like
PODXL
NM_005397
0.00303411
1.8943018
|
027
201617_x_at
caldesmon 1
CALD1
NM_004342
0.01926877
1.8294148
|
028
201646_at
scavenger receptor class B, member 2
SCARB2
AA885297
0.006063032
1.6768507
|
029
201647_s_at
scavenger receptor class B, member 2
SCARB2
NM_005506
0.015885489
1.6841809
|
030
201695_s_at
nucleoside phosphorylase
NP
NM_000270
0.018524641
1.6833633
|
031
201722_s_at
UDP-N-acetyl-alpha-D-galactosamine:polypeptide
GALNT1
AV692127
0.009770202
1.5369248
|
N-acetylgalactosaminyltransferase 1 (GalNAc-T1)
|
032
201918_at
Solute carrier family 25, member 36
SLC25A36
AI927944
0.00259865
1.6228764
|
033
201942_s_at
carboxypeptidase D
CPD
D85390
0.017363481
1.7431495
|
(melanoma growth stimulating activity, alpha)
|
034
202267_at
laminin, gamma 2
LAMC2
NM_005562
0.004330024
2.8191426
|
035
202543_s_at
glia maturation factor, beta
GMFB
BC005359
0.008048828
1.5254242
|
036
202604_x_at
ADAM metallopeptidase domain 10
ADAM10
NM_001110
0.002003783
1.767903
|
037
202627_s_at
serpin peptidase inhibitor, clade E (nexin,
SERPINE1
AL574210
0.00091248
3.0523725
|
plasminogen activator inhibitor type 1), member 1
|
038
202628_s_at
serpin peptidase inhibitor, clade E (nexin,
SERPINE1
NM_000602
0.00504642
2.6835847
|
plasminogen activator inhibitor type 1), member 1
|
039
202817_s_at
synovial sarcoma translocation, chromosome 18
SS18
NM_005637
0.005462693
1.5148987
|
040
202859_x_at
interleukin 8
IL8
NM_000584
0.014948112
2.1844351
|
041
202936_s_at
SRY (sex determining region Y)-box 9
SOX9
NM_000346
0.019816045
2.2876046
|
(campomelic dysplasia, autosomal sex-reversal)
|
042
202949_s_at
four and a half LIM domains 2
FHL2
NM_001450
0.006776552
2.2249734
|
043
202998_s_at
lysyl oxidase-like 2
LOXL2
NM_002318
0.006687925
2.0231075
|
044
203066_at
B cell RAG associated protein
GALNAC4S-6ST
NM_014863
0.00419499
1.5032523
|
045
203072_at
myosin IE
MYO1E
NM_004998
0.000449373
1.5877136
|
046
203293_s_at
lectin, mannose-binding, 1
LMAN1
NM_005570
0.002661762
1.9762497
|
047
203294_s_at
lectin, mannose-binding, 1
LMAN1
U09716
0.000473367
1.9764429
|
048
203414_at
monocyte to macrophage differentiation-associated
MMD
NM_012329
0.001585437
1.6128623
|
049
203553_s_at
mitogen-activated protein kinase kinase kinase kinase 5
MAP4K5
NM_006575
0.010453912
1.5251595
|
050
203924_at
glutathione S-transferase A1
GSTA1
NM_000846
0.004046575
4.2017674
|
051
203988_s_at
fucosyltransferase 8 (alpha (1,6) fucosyltransferase)
FUT8
NM_004480
0.01139016
1.6090198
|
052
204426_at
transmembrane emp24 domain trafficking protein 2
TMED2
NM_006815
0.015985437
1.6165011
|
053
204470_at
chemokine (C-X-C motif) ligand 1
CXCL1
NM_001511
0.001788037
3.218731
|
054
204702_s_at
nuclear factor (erythroid-derived 2)-like 3
NFE2L3
NM_004289
0.015985157
1.7023398
|
055
204790_at
SMAD, mothers against DPP homolog 7 (Drosophila)
SMAD7
NM_005904
0.013379821
1.7179344
|
056
204944_at
protein tyrosine phosphatase, receptor type, G
PTPRG
NM_002841
0.004963213
1.769544
|
057
204989_s_at
integrin, beta 4
ITGB4
BF305661
0.012746719
2.1320713
|
058
205120_s_at
sarcoglycan, beta (43 kDa dystrophin-associated glycoprotein)
SGCB
U29586
0.013908542
1.7317705
|
059
205180_s_at
ADAM metallopeptidase domain 8
ADAM8
NM_001109
0.000473816
2.054043
|
060
205479_s_at
plasminogen activator, urokinase
PLAU
NM_002658
0.003415823
2.4370956
|
061
206025_s_at
tumor necrosis factor, alpha-induced protein 6
TNFAIP6
AW188195
0.013965369
2.1515768
|
062
206113_s_at
RAB5A, member RAS oncogene family
RAB5A
NM_004162
0.010821017
1.571063
|
063
206116_s_at
tropomyosin 1 (alpha)
TPM1
NM_000366
0.000283653
2.0841253
|
064
206245_s_at
Influenza virus NS1A binding protein
IVNS1ABP
NM_006469
0.003607815
1.5105128
|
065
206323_x_at
oligophrenin 1
OPHN1
NM_002547
0.018292218
1.5056778
|
066
208510_s_at
peroxisome proliferative activated receptor, gamma
PPARG
NM_015869
0.002361554
1.882336
|
067
208613_s_at
filamin B, beta (actin binding protein 278)
FLNB
AV712733
0.001033398
1.7958127
|
068
208637_x_at
actinin, alpha 1
ACTN1
BC003576
0.000448714
1.631627
|
069
208653_s_at
CD164 antigen, sialomucin
CD164
AF263279
0.017487219
1.5380286
|
070
208853_s_at
calnexin
CANX
L18887
0.011792572
1.5100785
|
071
209131_s_at
synaptosomal-associated protein, 23 kDa
SNAP23
U55936
0.001730693
1.8878508
|
072
209209_s_at
pleckstrin homology domain containing, family C
PLEKHC1
AW469573
0.009551367
1.9820172
|
(with FERM domain) member 1
|
073
209314_s_at
HBS1-like (S. cerevisiae)
HBS1L
AK024258
0.00507411
1.6641864
|
074
209316_s_at
HBS1-like (S. cerevisiae)
HBS1L
BC001465
0.006051209
1.6464524
|
075
209409_at
growth factor receptor-bound protein 10
GRB10
DB6962
0.01098607
1.7481923
|
076
209410_s_at
growth factor receptor-bound protein 10
GRB10
AF000017
0.013879589
1.701537
|
077
209537_at
exostoses (multiple)-like 2
EXTL2
AF000416
0.003979554
1.5687809
|
078
210845_s_at
plasminogen activator, urokinase receptor
PLAUR
U08839
0.007479298
1.7924315
|
079
210892_s_at
general transcription factor II, i
GTF2I
BC004472
0.003141172
1.619537
|
080
210933_s_at
fascin homolog 1, actin-bundling protein
FSCN1
BC004908
0.00342191
1.906748
|
(Strongylocentrotus purpuratus)
|
081
210987_x_at
tropomyosin 1 (alpha)
TPM1
M19267
0.004614187
1.6935222
|
082
211299_s_at
flotillin 2
FLOT2
BC003683
0.015057402
1.5387125
|
083
211506_s_at
interleukin 8
IL8
AF043337
0.005428782
2.867063
|
084
211559_s_at
cyclin G2
CCNG2
L49506
0.010491861
1.8367761
|
085
211599_x_at
met proto-oncogene (hepatocyte growth factor receptor)
MET
U19348
0.019789577
1.9247686
|
086
211651_s_at
laminin, beta 1
LAMB1
M20206
0.000418344
1.997547
|
087
211668_s_at
plasminogen activator, urokinase
PLAU
K03226
0.00240352
2.8568754
|
088
211864_s_at
fer-1-like 3, myoferlin (C. elegans)
FER1L3
AF207990
0.011889962
1.7860718
|
089
211924_s_at
plasminogen activator, urokinase receptor
PLAUR
AY029180
0.011789334
1.8189595
|
090
211981_at
collagen, type IV, alpha 1
COL4A1
NM_001845
0.007531395
1.8490748
|
091
212012_at
peroxidasin homolog (Drosophila)
PXDN
BF342851
0.016265145
1.8463359
|
092
212660_at
PHD finger protein 15
PHF15
AI735639
0.007391165
1.5595657
|
093
212720_at
poly(A) polymerase alpha
PAPOLA
AI670847
0.016607396
1.5904158
|
094
212907_at
Solute carrier family 30 (zinc transporter), member 1
SLC30A1
AI972416
0.002460855
1.63999
|
095
213288_at
O-acyltransferase (membrane bound) domain containing 2
OACT2
AI761250
0.010427832
1.6232696
|
096
213457_at
malignant fibrous histiocytoma amplified sequence 1
MFHAS1
BF739959
0.003050241
1.8505166
|
097
213624_at
sphingomyelin phosphodiesterase, acid-like 3A
SMPDL3A
AA873600
0.005912889
1.8562527
|
098
213742_at
splicing factor, arginine/serine-rich 11
SFRS11
AW241752
0.006011819
1.9170463
|
099
214121_x_at
PDZ and LIM domain 7 (enigma)
PDLIM7
AA086229
5.50514E−05
1.5048952
|
100
214196_s_at
tripeptidyl peptidase I
TPP1
AA602532
0.015398935
1.5939685
|
101
214544_s_at
synaptosomal-associated protein, 23 kDa
SNAP23
NM_003825
0.003539713
1.8040004
|
102
214581_x_at
tumor necrosis factor receptor superfamily, member 21
TNFRSF21
BE568134
0.002274355
2.2189345
|
103
214701_s_at
fibronectin 1
FN1
AJ276395
0.001182322
2.071262
|
104
214866_at
plasminogen activator, urokinase receptor
PLAUR
X74039
0.003173471
1.7340106
|
105
214895_s_at
ADAM metallopeptidase domain 10
ADAM10
AU135154
0.004170008
1.9890832
|
106
215501_s_at
dual specificity phosphatase 10
DUSP10
AK022513
0.018290011
1.5388945
|
107
216035_x_at
transcription factor 7-like 2 (T-cell specific, HMG-box)
TCF7L2
AV721430
0.000657631
1.7091621
|
108
216511_s_at
transcription factor 7-like 2 (T-cell specific, HMG-box)
TCF7L2
AJ270770
0.004103699
1.5264177
|
109
216915_s_at
protein tyrosine phosphatase, non-receptor type 12
PTPN12
S69182
0.005493577
1.6935816
|
110
216971_s_at
plectin 1, intermediate filament binding protein 500 kDa
PLEC1
Z54367
0.01826363
1.7186335
|
111
217188_s_at
chromosome 14 open reading frame 1
C14orf1
AC007182
0.011925477
1.6185476
|
112
217448_s_at
chromosome 14 open reading frame 92
C14orf92
AL117508
0.007782524
1.5433311
|
similar to Epidermal Langerhans cell protein LCP1
LOC285412
|
113
217492_s_at
phosphatase and tensin homolog
PTEN
AF023139
0.007220107
1.5624946
|
(mutated in multiple advanced cancers 1)
|
114
218000_s_at
pleckstrin homology-like domain, family A, member 1
PHLDA1
NM_007350
0.016502094
1.6960312
|
115
218077_s_at
zinc finger, DHHC-type containing 3
ZDHHC3
BE542551
0.01684034
1.5417765
|
116
218078_s_at
zinc finger, DHHC-type containing 3
ZDHHC3
NM_016598
0.010970607
1.5836283
|
117
218435_at
DnaJ (Hsp40) homolog, subfamily C, member 15
DNAJC15
NM_013238
0.019865552
1.7292447
|
118
218644_at
pleckstrin 2
PLEK2
NM_016445
0.000675608
2.7071812
|
119
218748_s_at
SEC10-like 1 (S. cerevisiae)
SEC10L1
NM_006544
0.012352341
1.7368068
|
120
218815_s_at
transmembrane protein 51
TMEM51
NM_018022
0.000753902
1.6477742
|
121
218826_at
solute carrier family 35, member F2
SLC35F2
NM_017515
0.009280122
1.6340361
|
122
218854_at
squamous cell carcinoma
SART2
NM_013352
0.014419112
1.6285655
|
antigen recognized by T cells 2
|
123
218856_at
tumor necrosis factor receptor superfamily, member 21
TNFRSF21
NM_016629
0.01292243
1.617686
|
124
218885_s_at
UDP-N-acetyl-alpha-D-galactosamine:polypeptide
GALNT12
NM_024642
0.014052196
1.6402073
|
N-acetylgalactosaminyltransferase 12 (GalNAc-T12)
|
125
219410_at
transmembrane protein 45A
TMEM45A
NM_018004
0.018847797
2.0938365
|
126
219603_s_at
zinc finger protein 226
ZNF226
NM_015919
0.005593323
1.5408667
|
127
220199_s_at
chromosome 1 open reading frame 80
C1orf80
NM_022831
0.016323
1.5315142
|
128
220617_s_at
zinc finger protein 532
ZNF532
NM_018181
0.001976648
1.5441327
|
129
221268_s_at
sphingosine-1-phosphate phosphatase 1
SGPP1
NM_030791
0.008873873
1.9432548
|
130
221881_s_at
chloride intracellular channel 4
CLIC4
AI638420
0.004401053
1.7742935
|
131
222399_s_at
SM-11044 binding protein
SMBP
BG104571
0.00011337
1.5270268
|
132
222449_at
tranamembrane, prostate
TMEPAI
AL035541
0.005303006
2.2757804
|
androgen induced RNA
|
133
222528_s_at
solute carrier family 25, member 37
SLC25A37
BG251467
0.014745607
1.738053
|
134
222540_s_at
hepatitis B virus x associated protein
HBXAP
BG286920
0.005694628
1.5068418
|
135
222692_s_at
fibronectin type III domain containing 3B
FNDC3B
BF444916
0.001075083
1.5835624
|
136
222693_at
fibronectin type III domain containing 3B
FNDC3B
BF444916
0.000622161
1.7766397
|
137
222773_s_at
UDP-N-acetyl-alpha-D-galactosamine:polypeptide
GALNT12
AA554045
0.003090952
1.8790901
|
N-acetylgalactosaminyltransferase 12 (GalNAc-T12)
|
138
223577_x_at
PRO1073 protein
PRO1073
AA827878
0.003659447
1.6790042
|
139
223940_x_at
metastasis associated lung adenocarcinoma
MALAT1
AF132202
0.016841894
1.9524238
|
transcript 1 (non-coding RNA)
|
140
224558_s_at
metastasis associated lung adenocarcinoma
MALAT1
AI446756
0.012874936
1.6367766
|
transcript 1 (non-coding RNA)
|
141
224674_at
tweety homolog 3 (Drosophila)
TTYH3
AI934753
0.002428954
1.6452742
|
142
224733_at
chemokine-like factor superfamily 3
CKLFSF3
AL574900
0.013543638
1.5199631
|
143
224802_at
Nedd4 family interacting protein 2
NDFIP2
AA019338
0.013437813
1.5261155
|
144
225021_at
zinc finger protein 532
ZNF532
AA861416
0.002285053
1.6213596
|
145
225140_at
Kruppel-like factor 3 (basic)
KLF3
BF438116
0.016804362
1.5368354
|
146
225168_at
FERM domain containing 4A
FRMD4A
T78406
0.006987929
1.5712297
|
147
225424_at
glycerol-3-phosphate acyltransferase, mitochondrial
GPAM
AB046780
0.000390623
1.7006425
|
148
225503_at
dehydrogenase/reductase (SDR family) X-linked
DHRSX
AL547782
0.005000754
1.770981
|
149
225567_at
Hypothetical LOC388114
LOC388114
BE207755
0.003047524
1.6990312
|
150
225609_at
glutathione reductase
GSR
AI888037
0.004693668
1.8490914
|
151
225842_at
Pleckstrin homology-like domain, family A, member 1
PHLDA1
AK026181
0.014052763
1.8735564
|
152
226084_at
microtubule-associated protein 1B
MAP1B
AA554833
0.016480966
1.9064581
|
153
226352_at
Junction-mediating and regulatory protein
JMY
BF447037
0.001219355
1.5196482
|
154
226726_at
O-acyltransferase (membrane bound) domain containing 2
OACT2
W63676
0.005363467
1.8277074
|
155
226780_s_at
hypothetical protein HSPC268
HSPC268
BF540829
0.001859941
1.5185972
|
156
227257_s_at
chromosome 10 open reading frame 46
C10orf46
AW973842
0.000646104
1.6094143
|
157
227628_at
similar to RIKEN cDNA 2310016C16
LOC493869
AL571557
0.006222301
2.0978951
|
158
227808_at
DnaJ (Hsp40) homolog, subfamily C, member 15
DNAJC15
AI091398
0.01153802
1.7936606
|
159
230206_at
Dedicator of cytokinesis 5
DOCK5
AI692645
0.005127667
1.6694399
|
160
231735_s_at
PRO1073 protein
PRO1073
NM_014086
0.004784999
1.72546
|
161
231823_s_at
KIAA1295
KIAA1295
BG054798
0.002478401
1.5713933
|
162
235587_at
hypothetical protein LOC202781
LOC202781
BG400596
0.018314553
1.5202585
|
163
235879_at
Muscleblind-like (Drosophila)
MBNL1
AI697540
0.002645486
2.0540323
|
164
238558_at
Muscleblind-like (Drosophila)
MBNL1
AI445833
0.004576562
1.805269
|
165
238563_at
Ab1-interactor 1
ABI1
AV762916
0.012934915
1.6069295
|
166
238701_x_at
FLJ45803 protein
FLJ45803
BE176566
0.01719282
1.5133282
|
|
The gene expression pattern of the lung cancer cell classified into adenocarcinoma after lung cancer tissue removal operation is analyzed through a hybridization with the probe on the microarray, and a marker gene that is determined to have a difference in an expression level between a patient with lung cancer recurrence within one year (recurrence group) and a patient without lung cancer recurrence even after three years (non-recurrence group) is selected. The results are shown in Table 2 below. A total number of adenocarcinoma patients was 23. Among them, the number of patients with lung cancer recurrence within one year after lung cancer tissue removal operation was 8, and the number of patients without lung cancer recurrent even after three years was 15.
The gene expression pattern of the lung cancer cell classified into squamous cell carcinoma after lung cancer tissue removal operation is analyzed through a hybridization with the probe on the microarray, and a marker gene that is determined to have a difference in an expression level between a patient with lung cancer recurrence within one year (recurrence group) and a patient without lung cancer recurrence even after three years (non-recurrence group) is selected. The results are shown in Table 3 below. A total number of squamous cell carcinoma patients was 37. Among them, the number of patients with lung cancer recurrence within one year after lung cancer tissue removal operation was 11, and the number of patients without lung cancer recurrent even after three years was 26.
TABLE 2
|
|
T-test
Fold
|
Genbank
p-
change
|
NO
Probe Set ID
Gene Name
Gene Symbol
Accession #
value
(abs)
|
|
|
001
1553105_s_at
desmoglein 2
DSG2
NM_001943
0.01
5.339528
|
002
1553589_a_at
PDZK1 interacting protein 1
PDZK1IP1
NM_005764
0.02
3.608417
|
003
1553768_a_at
discoidln, CUB and LCCL domain containing 1
DCBLD1
NM_173674
0.01
1.9046342
|
004
1553928_at
ELMO domain containing 2
ELMOD2
NM_153702
0.02
1.7168769
|
005
1554327_a_at
calcium activated nucleotidase 1
CANT1
AF328554
0.02
1.6306834
|
006
1558685_a_at
hypothetical protein BC009467
LOC158980
BC009467
0.03
1.6841992
|
007
1559399_s_at
zinc finger, CCHC domain containing 10
ZCCHC10
BC015988
0.02
1.5219704
|
008
1568578_s_at
FGFR1 oncogene partner
FGFR1OP
BC037785
0.01
2.4856193
|
009
160020_at
matrix metallopeptidase 14 (membrane-inserted)
MMP14
Z48481
0.03
1.8354192
|
010
200730_s_at
protein tyrosine phosphatase type IVA, member 1
PTP4A1
BF576710
0.03
2.6575127
|
011
200733_s_at
protein tyrosine phosphatase type IVA, member 1
PTP4A1
U48296
0.02
1.5593889
|
012
200864_s_at
RAB11A, member RAS oncogene family
RAB11A
NM_004663
0.02
1.6270655
|
013
200890_s_at
signal sequence receptor, alpha
SSR1
AW006345
0.01
1.8127153
|
(translocon-associated protein alpha)
|
014
200931_s_at
vinculin
VCL
NM_014000
0.01
1.7692009
|
015
201011_at
ribophorin I
RPN1
NM_002950
0.01
1.6075972
|
016
201106_at
glutathione peroxidase 4
GPX4
NM_002085
0.02
1.6833277
|
(phospholipid hydroperoxidase)
|
017
201143_s_at
eukaryotic translation initiation factot 2.
EIF2S1
BC002513
0.02
2.298374
|
subunit 1 alpha, 35 kDa
|
018
201207_at
tumor necrosis factor, alpha-induced protein 1 (endothelial)
TNFAIP1
NM_021137
0.01
1.6828994
|
019
201250_s_at
solute carrier family 2
SLC2A1
NM_006516
0.02
4.009399
|
(facilitated glucose transporter), member 1
|
020
201392_s_at
insulin-like growth factor 2 receptor
IGF2R
BG031974
0.02
1.6488191
|
021
201393_s_at
insulin-like growth factor 2 receptor
IGF2R
NM_000876
0.02
1.5784883
|
022
201456_s_at
BUB3 budding uninhibited by
BUB3
AU160695
0.01
1.7238452
|
benzimidazoles 3 homolog (yeast)
|
023
201458_s_at
BUB3 budding uninhibited by
BUB3
NM_004725
0.01
1.5530633
|
benzimidazoles 3 homolog (yeast)
|
024
201525_at
apolipoprotein D
APOD
NM_001647
0.03
4.186704
|
025
201564_s_at
fascin homolog 1, actin-bundling protein
FSCN1
NM_003088
0.01
3.2328043
|
(Strongylocentrotus purpuratus)
|
026
201631_s_at
immediate early response 3
IER3
NM_003897
0.01
3.0016828
|
027
201656_at
integrin, alpha 6
ITGA6
NM_000210
0.01
2.3616688
|
028
201700_at
cyclin D3
CCND3
NM_001760
0.02
1.6460308
|
029
202047_s_at
chromobox homolog 6
CBX6
AI458128
0.01
1.9611783
|
030
202048_s_at
chromobox homolog 6
CBX6
NM_014292
0.02
1.6010046
|
031
202086_at
myxovirus (influenza virus) resistance 1,
MX1
NM_002462
0.02
2.4754105
|
interferon-inducible protein p78 (mouse)
|
032
202130_at
RIO kinase 3 (yeast)
RIOK3
AA725102
0.01
1.6167943
|
033
202131_s_at
RIO kinase 3 (yeast)
RIOK3
NM_003831
0.02
1.7833867
|
034
202233_s_at
ubiquinol-cytochrome c reductase hinge protein
UQCRH
NM_006004
0.03
1.5353662
|
035
202267_at
laminin, gamma 2
LAMC2
NM_005562
0.01
3.9229517
|
036
202293_at
stromal antigen 1
STAG1
AW168948
0.01
1.7993419
|
037
202604_x_at
ADAM metallopeptidase domain 10
ADAM10
NM_001110
0.02
2.0231702
|
038
202696_at
oxidative-stress responsive 1
OXSR1
NM_005109
0.03
1.5418515
|
039
202816_s_at
synovial sarcoma translocation, chromosome 18
SS18
AW292882
0.01
2.0899003
|
040
202856_s_at
solute carrier family 16 (monocarboxylic acid transporters),
SLC16A3
NM_004207
0.01
2.8914852
|
member 3
|
041
202869_at
2′,5′-oligoadenylate synthetase 1, 40/46 kDa
OAS1
NM_016816
0.02
3.431309
|
042
202887_s_at
DNA-damage-inducible transcript 4
DDIT4
NM_019058
0.02
2.74081
|
043
202904_s_at
LSM5 homolog, U6 small nuclear RNA associated (S. cerevisiae)
LSM5
NM_012322
0.03
1.8907431
|
044
202934_at
hexokinase 2
HK2
AI761561
0.01
2.1517375
|
045
203072_at
myosin IE
MYO1E
NM_004998
0.01
2.039332
|
046
203177_x_at
transcription factor A, mitochondrial
TFAM
NM_003201
0.02
1.8601428
|
047
203256_at
cadherin 3, type 1, P-cadherin (placental)
CDH3
NM_001793
0.01
2.6757588
|
048
203287_at
ladinin 1
LAD1
NM_005558
0.03
1.9237865
|
049
203311_s_at
ADP-ribosylation factor 6
ARF6
M57763
0.02
1.9452083
|
050
203313_s_at
TGFB-induced factor (TALE family homeobox)
TGIF
NM_003244
0.01
1.5528815
|
051
203344_s_at
retinoblastoma binding protein 8
RBBP8
NM_002894
0.01
1.7286093
|
052
203395_s_at
hairy and enhancer of split 1, (Drosophila)
HES1
NM_005524
0.02
1.6101321
|
053
203430_at
heme binding protein 2
HEBP2
NM_014320
0.02
1.822933
|
054
203476_at
trophoblast glycoprotein
TPBG
NM_006670
0.03
2.0313597
|
055
203499_at
EPH receptor A2
EPHA2
NM_004431
0.01
2.4758015
|
056
203501_at
plasma glutamate carboxypeptidase
PGCP
NM_006102
0.02
1.742001
|
057
203535_at
S100 calcium binding protein A9 (calgranulin B)
S100A9
NM_002965
0.02
5.647521
|
058
203554_x_at
pituitary tumor-transforming 1
PTTG1
NM_004219
0.02
2.1384234
|
059
203642_s_at
COBL-like 1
COBLL1
NM_014900
0.02
1.7199888
|
060
203690_at
tubulin, gamma complex associated protein 3
TUBGCP3
NM_006322
0.01
1.6228286
|
061
203906_at
IQ motif and Sec7 domain 1
IQSEC1
AI652645
0.01
1.7168043
|
062
203964_at
N-myc (and STAT) interactor
NMI
NM_004688
0.01
1.8720082
|
063
203988_s_at
fucosyltransferase 8 (alpha (1,6) fucosyltransferase)
FUT8
NM_004480
0.01
2.0948534
|
064
204136_at
collagen, type VII, alpha 1 (epidermolysis bullosa,
COL7A1
NM_000094
0.01
2.2071517
|
dystrophic, dominant and recessive)
|
065
204401_at
potassium intermediate/small conductance
KCNN4
NM_002250
0.01
3.260382
|
calcium-activated channel, subfamily N, member 4
|
066
204415_at
interferon, alpha-inducible protein (clone IFI-6-16)
G1P3
NM_022873
0.02
4.0747566
|
067
204470_at
chemokine (C-X-C motif) ligand 1
CXCL1
NM_001511
0.01
6.7313213
|
(melanoma growth stimulating activity, alpha)
|
068
204580_at
matrix metallopeptidase 12 (macrophage elastase)
MMP12
NM_002426
0.02
7.360193
|
069
204587_at
solute carrier family 25 (mitochondrial carrier, brain), member 14
SLC25A14
NM_003951
0.02
1.5086871
|
070
204616_at
ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase)
UCHL3
NM_006002
0.03
1.8766123
|
071
204635_at
ribosomal protein S6 kinase, 90 kDa, polypeptide 5
RPS6KA5
NM_004755
0.01
1.853935
|
072
204747_at
interferon-induced protein with tetratricopeptide repeats 3
IFIT3
NM_001549
0.02
2.588765
|
073
204809_at
ClpX caseinolytic peptidase X homolog (E. coli)
CLPX
NM_006660
0.02
1.5264844
|
074
204857_at
MAD1 mitotic arrest deficient-like 1 (yeast)
MAD1L1
NM_003550
0.03
1.6594671
|
075
204875_s_at
GDP-mannose 4,6-dehydratase
GMDS
NM_001500
0.02
2.5758607
|
076
204990_s_at
integrin, beta 4
ITGB4
NM_000213
0.01
3.176456
|
077
205004_at
NF-kappaB repressing factor
NKRF
NM_017544
0.02
1.5878501
|
078
205016_at
transforming growth factor, alpha
TGFA
NM_003236
0.01
2.1914852
|
079
205120_s_at
sarcoglycan, beta (43 kDa dystrophin-associated glycoprotein)
SGCB
U29586
0.01
2.5721073
|
080
205157_s_at
keratin 17
KRT17
NM_000422
0.01
5.252511
|
081
205180_s_at
ADAM metallopeptidase domain 8
ADAM8
NM_001109
0.01
2.1361954
|
082
205202_at
protein-L-isoaspartate (D-aspartate) O-methyltransferase
PCMT1
NM_005389
0.01
1.5924072
|
083
205339_at
TAL1 (SCL) interrupting locus
SIL
NM_003035
0.02
2.043193
|
084
205455_at
macrophage stimulating 1 receptor (c-met-related tyrosine kinase)
MST1R
NM_002447
0.02
2.835629
|
085
205479_s_at
plasminogen activator, urokinase
PLAU
NM_002658
0.01
3.8200433
|
086
205518_s_at
cytidine monophosphate-N-acetylneuraminic acid
CMAH
NM_003570
0.01
2.596108
|
hydroxy (CMP-N-acetylneuraminate monooxygenase)
|
087
205945_at
interleukin 6 receptor
IL6R
NM_000565
0.03
1.8261979
|
088
206055_s_at
small nuclear ribonucleoprotein polypeptide A′
SNRPA1
NM_003090
0.01
1.5232844
|
089
206323_x_at
oligophrenin 1
OPHN1
NM_002547
0.01
2.3268037
|
090
206414_s_at
development and differentiation enhancing factor 2
DDEF2
NM_003887
0.01
2.089077
|
091
207079_s_at
mediator of RNA polymerase II transcription,
MED6
NM_005466
0.03
1.8905708
|
subunit 6 homolog (yeast)
|
092
207850_at
chemokine (C-X-C motif) ligand 3
CXCL3
NM_002090
0.02
4.294934
|
093
208091_s_at
EGFR-coamplified and overexpressed protein
ECOP
NM_030796
0.02
2.2340379
|
094
208613_s_at
filamin B, beta (actin binding protein 278)
FLNB
AV712733
0.01
2.3647172
|
095
208636_at
Actinin, alpha 1
ACTN1
AI082078
0.01
1.8102713
|
096
208637_x_at
actinin, alpha 1
ACTN1
BC003576
0.01
2.062581
|
097
208819_at
RAB8A, member RAS oncogene family
RAB8A
BC002977
0.01
1.6729795
|
098
208840_s_at
Ras-GTPase activating protein
G3BP2
AU149503
0.02
1.8072606
|
SH3 domain-binding protein 2
|
099
208875_s_at
p21 (CDKN1A)-activated kinase 2
PAK2
BF796470
0.01
2.1095228
|
100
208876_s_at
p21 (CDKN1A)-activated kinase 2
PAK2
AI076186
0.02
1.6706929
|
101
208878_s_at
p21 (CDKN1A)-activated kinase 2
PAK2
AF092132
0.02
1.5662557
|
102
209022_at
stromal antigen 2
STAG2
AK026678
0.01
1.5019888
|
103
209025_s_at
synaptotagmin binding, cytoplasmic RNA interacting protein
SYNCRIP
AF037448
0.01
1.748127
|
104
209314_s_at
HBS1-like (S. cerevisiae)
HBS1L
AK024258
0.01
2.2400491
|
105
209417_s_at
interferon-induced protein 35
IFI35
BC001356
0.02
1.9908478
|
106
209476_at
thioredoxin domain containing
TXNDC
AL080080
0.02
1.5641398
|
107
209487_at
RNA binding protein with multiple splicing
RBPMS
D84109
0.02
1.5929683
|
108
209537_at
exostoses (multiple)-like 2
EXTL2
AF000416
0.03
2.019564
|
109
209627_s_at
oxysterol binding protein-like 3
OSBPL3
AY008372
0.03
1.9842228
|
110
209791_at
peptidyl arginine deiminase, type II
PADI2
AL049569
0.02
1.5902214
|
111
210092_at
mago-nashi homolog,
MAGOH
AF067173
0.03
1.7290384
|
proliferation-associated (Drosophila)
|
112
210093_s_at
mago-nashi homolog, proliferation-associated (Drosophila)
MAGOH
AF067173
0.01
1.5214177
|
113
210104_at
mediator of RNA polymerase II transcription.
MED6
AF074723
0.01
1.7416326
|
subunit 6 homolog (yeast)
|
114
210273_at
BH-protocadherin (brain-heart)
PCDH7
AB006757
0.03
1.5068512
|
115
210933_s_at
fascin homolog 1, actin-bundling protein (Strongylocentrotus purpuratus)
FSCN1
BC004908
0.01
2.660472
|
116
211160_x_at
actinin, alpha 1
ACTN1
M95178
0.01
1.6758434
|
117
211668_s_at
plasminogen activator, urokinase
PLAU
K03226
0.03
4.548989
|
118
211737_x_at
pleiotrophin
PTN
BC005916
0.02
2.2613049
|
(heparin binding growth factor 8, neurite growth-promoting factor 1)
|
119
212203_x_at
interferon induced transmembrane protein 3 (1-8U)
IFITM3
BF338947
0.01
1.5134683
|
120
212221_x_at
iduronate 2-sulfatase (Hunter syndrome)
IDS
AV703259
0.01
1.8884305
|
121
212236_x_at
keratin 17
KRT17
Z19574
0.01
3.7909358
|
122
212268_at
serpin peptidase inhibitor, clade B (ovalbumin), member 1
SERPINB1
NM_030666
0.02
1.9949495
|
123
212312_at
BCL2-like 1
BCL2L1
AL117381
0.02
1.5705433
|
124
212322_at
sphingosine-1-phosphate lyase 1
SGPL1
BE999972
0.01
1.6549215
|
125
212330_at
transcription factor Dp-1
TFDP1
R60866
0.02
2.1620867
|
126
212531_at
lipacalin 2 (oncogene 24p3)
LCN2
NM_005564
0.02
6.2857018
|
127
212657_s_at
interleukin 1 receptor antagonist
IL1RN
U65590
0.02
3.7755005
|
128
212858_at
progestin and adipoQ receptor family member IV
PAQR4
AL520675
0.01
2.2580597
|
129
212992_at
chromosome 14 open reading frame 78
C14orf78
AI935123
0.01
5.9573503
|
130
213088_s_at
DnaJ (Hsp40) homolog, subfamily C, member 9
DNAJC9
BE551340
0.02
1.784215
|
131
213288_at
O-acyltransferase (membrane bound) domain containing 2
OACT2
AI761250
0.02
2.1144574
|
132
214121_x_at
PDZ and LIM domain 7 (enigma)
PDLIM7
AA086229
0.01
1.7699668
|
133
214453_s_at
interferon-induced protein 44
IFI44
NM_006417
0.03
2.8858101
|
134
214697_s_at
ROD1 regulator of differentiation 1 (S. pombe)
ROD1
AW190873
0.01
2.048636
|
135
214974_x_at
chemokine (C-X-C motif) ligand 5
CXCL5
AK026546
0.02
6.4936213
|
136
215223_s_at
superoxide dismutase 2, mitochondrial
SOD2
W46388
0.01
3.1782749
|
137
215230_x_at
eukaryotic translation initiation factor 3, subunit 8, 110 kDa
EIF3S8
AA679705
0.02
1.6019442
|
138
215411_s_at
TRAF3 interacting protein 2
TRAF3IP2
AL008730
0.03
1.72815
|
139
216153_x_at
reversion-inducing-cysteine-rich
RECK
AK022897
0.01
1.9417262
|
protein with kazal motifs
|
140
216841_s_at
superoxide dismutase 2, mitochondrial
SOD2
X15132
0.01
2.8182118
|
141
216905_s_at
suppression of tumorigenicity 14 (colon carcinoma, matriptase, epithin)
ST14
U20428
0.02
1.8127093
|
142
216977_x_at
small nuclear ribonucleoprotein polypeptide A′
SNRPA1
AJ130972
0.01
1.5991035
|
143
217834_s_at
synaptotagmin binding, cytoplasmic RNA interacting protein
SYNCRIP
NM_006372
0.03
1.7178055
|
144
217867_x_at
beta-site APP-cleaving enzyme 2
BACE2
NM_012105
0.01
2.5611665
|
145
217901_at
Desmoglein 2
DSG2
BF031829
0.01
3.4549432
|
146
218012_at
TSPY-like 2
TSPYL2
NM_022117
0.01
1.6316599
|
147
218288_s_at
hypothetical protein MDS025
MDS025
NM_021825
0.01
1.7013886
|
148
218294_s_at
nucleoporin 50 kDa
NUP50
AF267865
0.01
1.5833666
|
149
218400_at
2′-5′-oligoadenylate synthetase 3, 100 kDa
OAS3
NM_006187
0.01
3.0217175
|
150
218451_at
CUB domain containing protein 1
CDCP1
NM_022842
0.01
3.0102131
|
151
218460_at
hypothetical protein FLJ20397
FLJ20397
NM_017802
0.02
1.6881874
|
152
218498_s_at
ERO1-like (S. cerevisiae)
ERO1L
NM_014584
0.01
2.5205412
|
153
218573_at
melanoma antigen family H, 1
MAGEH1
NM_014061
0.02
1.6212198
|
154
218585_s_at
denticleless homolog (Drosophila)
DTL
NM_016448
0.03
2.4223747
|
155
218644_at
pleckstrin 2
PLEK2
NM_016445
0.01
4.898943
|
156
218796_at
chromosome 20 open reading frame 42
C20orf42
NM_017671
0.02
3.3694396
|
157
218826_at
solute carrier family 35, member F2
SLC35F2
NM_017515
0.03
2.0183008
|
158
218943_s_at
DEAD (Asp-Glu-Ala-Asp) box polypeptide 58
DDX58
NM_014314
0.02
2.4575703
|
159
218950_at
centaurin, delta 3
CENTD3
NM_022481
0.02
1.5173771
|
160
219146_at
chromosome 17 open reading frame 42
C17orf42
NM_024683
0.02
1.5234692
|
161
219296_at
zinc finger, DHHC-type containing 13
ZDHHC13
NM_019028
0.03
1.5033884
|
162
219303_at
chromosome 13 open reading frame 7
C13orf7
NM_024546
0.03
1.5534021
|
163
219332_at
MICAL-like 2
MICAL-L2
NM_024723
0.02
1.8410143
|
164
219399_at
lin-7 homolog C (C. elegans)
LIN7C
NM_018362
0.03
1.5852816
|
165
219421_at
osmosis responsive factor
OSRF
NM_012382
0.01
1.531867
|
166
219439_at
core 1 synthase, glycoprotein-N-acetylgalactosamine
C1GALT1
NM_020156
0.02
2.2143774
|
3-beta-galactosyltransferase, 1
|
167
219517_at
elongation factor RNA polymerase II-like 3
ELL3
NM_025165
0.02
1.6594616
|
168
219549_s_at
reticulon 3
RTN3
NM_006054
0.02
1.6491096
|
169
219603_s_at
zinc finger protein 226
ZNF226
NM_015919
0.01
1.8911394
|
170
219630_s_at
PDZK1 interacting protein 1
PDZK1IP1
NM_005764
0.02
3.5720232
|
171
219691_at
sterile alpha motif domain containing 9
SAMD9
NM_017654
0.01
2.2009485
|
172
219787_s_at
epithelial cell transforming sequence 2 oncogene
ECT2
NM_018098
0.02
3.414079
|
173
219799_s_at
dehydrogenase/reductase (SDR family) member 9
DHRS9
NM_005771
0.02
1.7866958
|
174
219959_at
molybdenum cofactor sulfurase
MOCOS
NM_017947
0.01
3.192601
|
175
220232_at
stearoyl-CoA desaturase 5
SCD5
NM_024906
0.01
3.2719014
|
176
220368_s_at
KIAA2010
KIAA2010
NM_017936
0.02
1.6052217
|
177
220725_x_at
Dynein, axonemal, heavy polypeptide 3
DNAH3
NM_025095
0.01
1.8525391
|
178
221477_s_at
hypothetical protein MGC5618
MGC5618
BF575213
0.01
2.2014346
|
179
221482_s_at
cyclic AMP phosphoprotein, 19 kD
ARPP-19
BC003418
0.02
1.711658
|
180
221732_at
calcium activated nucleotidase 1
CANT1
AK026161
0.02
1.6711121
|
181
221752_at
Slingshot homolog 1 (Drosophila)
SSH1
AL041728
0.02
1.678051
|
182
221922_at
G-protein signalling modulator 2 (AGS3-like, C. elegans)
GPSM2
AW195581
0.01
2.2638144
|
183
222392_x_at
PERP, TP53 apoptosis effector
PERP
AJ251830
0.02
1.8814404
|
184
222399_s_at
SM-11044 binding protein
SMBP
BG104571
0.02
1.6986449
|
185
222424_s_at
nuclear casein kinase and cyclin-dependent kinase substrate 1
NUCKS1
BC000805
0.01
1.6469624
|
186
222446_s_at
beta-site APP-cleaving enzyme 2
BACE2
AF178532
0.01
1.9711965
|
187
222492_at
pyridoxal (pyridoxine, vitamin B6) kinase
PDXK
AW262867
0.01
1.5873553
|
188
222502_s_at
ubiquitin-fold modifier 1
UFM1
BC005193
0.02
1.7238611
|
189
222523_at
SUMO1/sentrin/SMT3 specific peptidase 2
SENP2
BE622841
0.03
1.7830018
|
190
222528_s_at
solute carrier family 25, member 37
SLC25A37
BG251467
0.02
2.6761055
|
191
222561_at
LanC lantibiotic synthetase component C-like 2 (bacterial)
LANCL2
AJ278245
0.03
2.2797666
|
192
222587_s_at
UDP-N-acetyl-alpha-D-galactosamine:polypeptide
GALNT7
BF699855
0.03
1.7439753
|
N-acetylgalactosaminyltransferase 7(GalNAc-T7)
|
193
222689_at
phytoceramidase, alkaline
PHCA
N51263
0.01
1.7877864
|
194
222692_s_at
fibronectin type III domain containing 3B
FNDC3B
BF444916
0.01
1.9685304
|
195
222693_at
fibronectin type III domain containing 3B
FNDC3B
BF444916
0.02
2.1501522
|
196
222793_at
DEAD (Asp-Glu-Ala-Asp) box polypeptide 58
DDX58
AK023661
0.01
2.2502613
|
197
223219_s_at
CCR4-NOT transcription complex, subunit 10
CNOT10
BC002931
0.01
1.5173706
|
198
223278_at
gap junction protein, beta 2, 26 kDa (connexin 26)
GJB2
M86849
0.02
5.1083236
|
199
223374_s_at
UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase, polypeptide 3
B3GALT3
AF154848
0.02
2.124231
|
200
223421_at
cysteine/histidine-rich 1
CYHR1
BC005073
0.01
1.7838429
|
201
223467_at
RAS, dexamethasone-induced 1
RASD1
AF069506
0.01
3.1274104
|
202
223626_x_at
family with sequence similarity 14, member A
FAM14A
AF208232
0.01
1.5701514
|
203
223631_s_at
chromosome 19 open reading frame 33
C19orf33
AF213678
0.02
3.90325
|
204
224159_x_at
tripartite motif-containing 4
TRIM4
AF220023
0.01
2.2881489
|
205
224493_x_at
chromosome 18 open reading frame 45
C18orf45
BC006280
0.02
1.571958
|
206
224494_x_at
dehydrogenase/reductase (SDR family) member 10
DHRS10
BC006283
0.02
1.9102337
|
207
224564_s_at
reticulon 3
RTN3
BE544689
0.01
1.583082
|
208
224595_at
solute carrier family 44, member 1
SLC44A1
AK022549
0.01
1.601491
|
209
224596_at
solute carrier family 44, member 1
SLC44A1
AI634866
0.01
1.5728544
|
210
224598_at
mannosyl (alpha-1,3-)-glycoprotein beta-1,4-N-
MGAT4B
BF570193
0.03
1.5535489
|
acetylglucosaminyltransferase, isoenzyme B
|
211
224674_at
tweety homolog 3 (Drosophila)
TTYH3
AI934753
0.02
2.123153
|
212
224675_at
mesoderm development candidate 2
MESDC2
AK026606
0.01
1.6605617
|
213
224679_at
mesoderm development candidate 2
MESDC2
BE963495
0.01
1.65804
|
214
224681_at
guanine nucleotide binding protein (G protein) alpha 12
GNA12
BG028884
0.01
1.6103705
|
215
224799_at
Nedd4 family interacting protein 2
NDFIP2
AW290956
0.02
1.9774225
|
216
224802_at
Nedd4 family interacting protein 2
NDFIP2
AA019338
0.02
1.6960912
|
217
224827_at
Dendritic cell-derived ubiquitin-like protein
DC-UbP
AK022894
0.01
1.5073498
|
218
224902_at
pyruvate dehydrogenase phosphatase regulatory subunit
PDPR
BE644918
0.02
1.6357323
|
219
224950_at
prostaglandin F2 receptor negative regulator
PTGFRN
BF476250
0.03
1.9777663
|
220
225071_at
chromosome 6 open reading frame 68
C6orf68
BG168247
0.03
1.6909997
|
221
225272_at
spermidine/spermine N1-acetyltransferase 2
SAT2
AA128261
0.01
1.6911607
|
222
225331_at
chromosome 3 open reading frame 6
C3orf6
BF941088
0.02
2.126105
|
223
225342_at
adenylate kinase 3-like 1
AK3L1
AK026966
0.01
7.1160383
|
224
225366_at
phosphoglucomutase 2
PGM2
AI652855
0.03
1.527827
|
225
225375_at
chromosome 17 open reading frame 32
C17orf32
AW975808
0.02
1.8780395
|
226
225380_at
hypothetical protein BC007901
LOC91461
BF528878
0.02
2.6365216
|
227
225383_at
zinc finger protein 275
ZNF275
BF793625
0.01
1.639558
|
228
225547_at
HBII-276 host gene
HBII-276HG
BG169443
0.01
1.6269366
|
229
225550_at
AV700816
0.01
1.6167612
|
230
225571_at
leukemia inhibitory factor receptor
LIFR
AA701657
0.03
3.5799398
|
231
225575_at
leukemia inhibitory factor receptor
LIFR
AI680541
0.01
3.1433964
|
232
225578_at
similar to RIKEN cDNA 2410129H14
LOC440145
AI885466
0.01
1.8692675
|
233
225750_at
ERO1-like (S. cerevisiae)
ERO1L
BE966748
0.02
2.0413787
|
234
225842_at
Pleckstrin homology-like domain, family A, member 1
PHLDA1
AK026181
0.02
2.5619717
|
235
225847_at
arylacetamide deacetylase-like 1
AADACL1
AB037784
0.02
1.6796919
|
236
226060_at
RFT1 homolog (S. cerevisiae)
RFT1
BF475369
0.02
1.5211235
|
237
226112_at
sarcoglycan, beta (43 kDa dystrophin-associated glycoprotein)
SGCB
AI678717
0.01
1.5416645
|
238
226278_at
hypothetical protein DKFZp313A2432
DKFZp313A2432
AI150224
0.02
1.6910942
|
239
226335_at
ribosomal protein S6 kinase, 90 kDa, polypeptide 3
RPS6KA3
BG498334
0.01
1.8176109
|
240
226352_at
Junction-mediating and regulatory protein
JMY
BF447037
0.01
2.4128768
|
241
226488_at
RCC1 domain containing 1
RCCD1
AW007826
0.03
1.777583
|
242
226568_at
hypothetical protein LOC284611
LOC284611
AI478747
0.01
2.1426997
|
243
226609_at
discoidin, CUB and LCCL domain containing 1
DCBLD1
N22751
0.01
2.0089936
|
244
226702_at
hypothetical protein LOC129607
LOC129607
AI742057
0.01
2.5539525
|
245
226722_at
family with sequence similarity 20, member C
FAM20C
BE874872
0.01
2.2937167
|
246
226726_at
O-acyltransferase (membrane bound) domain containing 2
OACT2
W63676
0.01
2.8518102
|
247
226778_at
Chromosome 8 open reading frame 42
C8orf42
AI632224
0.02
1.9250498
|
248
226780_s_at
hypothetical protein HSPC268
HSPC268
BF540829
0.01
1.8384567
|
249
226781_at
hypothetical protein HSPC268
HSPC268
BF540829
0.01
1.7917764
|
250
226784_at
TWIST neighbor
TWISTNB
AA121481
0.01
1.750498
|
251
226832_at
Hypothetical LOC389188
LOC389188
BF978778
0.01
1.538109
|
252
226863_at
Full-length cDNA clone CS0DJ001YJ05 of T cells
AI674565
0.01
3.1555974
|
(Jurkat cell line) Cot 10-normalized of Homo sapiens (human)
|
253
226926_at
dermokine
ZD52F10
AA706316
0.02
3.190141
|
254
227141_at
chromosome 1 open reading frame 171
C1orf171
AW205739
0.02
1.6063374
|
255
227148_at
pleckstrin homology domain containing, family H
PLEKHH2
AI913749
0.03
2.1525955
|
(with MyTH4 domain) member 2
|
256
227172_at
hypothetical protein BC000282
LOC89894
BC000282
0.02
1.9858925
|
257
227249_at
AI857685
0.01
1.9229563
|
258
227314_at
Integrin, alpha 2 (CD49B, alpha 2 subunit of VLA-2 receptor)
ITGA2
N95414
0.03
3.3500278
|
259
227393_at
transmembrane protein 16J
TMEM16J
AW084755
0.01
1.6880668
|
260
227466_at
hypothetical protein LOC285550
LOC285550
BF108695
0.02
1.5282669
|
261
227771_at
leukemia inhibitory factor receptor
LIFR
AW592684
0.01
2.7902896
|
262
227808_at
DnaJ (Hsp40) homolog, subfamily C, member 15
DNAJC15
AI091398
0.03
1.8649827
|
263
227998_at
S100 calcium binding protein A16
S100A16
AA045184
0.01
2.2575665
|
264
228152_s_at
hypothetical protein FLJ31033
FLJ31033
AK023743
0.02
2.2769616
|
265
228275_at
CDNA FLJ32438 fis,
AI200555
0.02
1.813842
|
clone SKMUS2001402
|
266
228531_at
sterile alpha motif domain containing 9
SAMD9
AA741307
0.02
2.303081
|
267
228562_at
Zinc finger and BTB domain containing 10
ZBTB10
N29918
0.01
2.046323
|
268
228600_x_at
hypothetical protein MGC72075
MGC72075
BE220330
0.02
1.6221175
|
269
228640_at
BH-protocadherin (brain-heart)
PCDH7
BE644809
0.03
3.3346767
|
270
228713_s_at
dehydrogenase/reductase (SDR family) member 10
DHRS10
AI742586
0.02
1.9451209
|
271
228854_at
Transcribed locus
AI492388
0.03
4.4617124
|
272
228972_at
AI028602
0.02
1.6522069
|
273
229573_at
Transcribed locus
AI659456
0.02
1.5438964
|
274
229582_at
chromosome 18 open reading frame 37
C18orf37
AI758919
0.01
1.6219943
|
275
229997_at
vang-like 1 (van gogh, Drosophila)
VANGL1
AA789332
0.02
1.6355668
|
276
230206_at
Dedicator of cytokinesis 5
DOCK5
AI692645
0.01
1.7685658
|
277
230329_s_at
nudix (nucleoside diphosphate linked moiety X)-type motif 6
NUDT6
AI580268
0.02
1.5125636
|
278
230655_at
Homo sapiens, clone IMAGE: 5418468, mRNA
AW025928
0.01
2.44095
|
279
230972_at
ankyrin repeat domain 9
ANKRD9
AW194999
0.01
1.875526
|
280
231828_at
Homo sapiens, clone IMAGE: 5218355, mRNA
AL117474
0.02
2.1623232
|
281
231832_at
UDP-N-acetyl-alpha-D-galactosamine:polypeptide
GALNT4
AI890347
0.01
1.8446548
|
N-acetylgalactosaminyltransferase 4 (GalNAc-T4)
|
282
234675_x_at
CDNA: FLJ23566 fis, clone LNG10880
AK027219
0.01
2.4514613
|
283
234725_s_at
sema domain, immunoglobulin domain (lg), transmembrane domain
SEMA4B
AK026133
0.01
1.9406958
|
(TM) and short cytoplasmic domain, (semaphorin) 4B
|
284
235015_at
Zinc finger, DHHC-type containing 9
ZDHHC9
AL529434
0.01
2.4835925
|
285
235019_at
carboxypeptidase M
CPM
BE878495
0.02
3.833762
|
286
235096_at
Leo1, Paf1/RNA polymerase II complex component,
LEO1
AA074729
0.01
1.5779704
|
homolog (S. cerevisiae)
|
287
235648_at
zinc finger protein 567
ZNF567
AA742659
0.02
1.6336213
|
288
235911_at
hypothetical gene supported by BC034933; BC068085
LOC440995
AI885815
0.01
4.651685
|
289
238063_at
hypothetical protein FLJ32028
FLJ32028
AA806283
0.01
2.002421
|
290
238523_at
chromosome 16 open reading frame 44
C16orf44
BF941204
0.03
1.5099897
|
291
238701_x_at
FLJ45803 protein
FLJ45803
BE176566
0.01
2.3077648
|
292
238778_at
membrane protein, palmitoylated 7 (MAGUK p55 subfamily member 7)
MPP7
AI244661
0.02
3.0538154
|
293
239896_at
Similar to RAB guanine nucleotide exchange factor (GEF) 1
LOC402671
AW190479
0.02
1.6268736
|
294
241994_at
Xanthine dehydrogenase
XDH
BG260086
0.02
3.2672102
|
295
241996_at
AI669591
0.01
1.7369617
|
296
244495_x_at
chromosome 18 open reading frame 45
C18orf45
AL521157
0.01
1.8056976
|
297
36553_at
acetylserotonin O-methyltransferase-like
ASMTL
AA669799
0.02
1.6164968
|
298
36829_at
period homolog 1 (Drosophila)
PER1
AF022991
0.01
1.9640467
|
299
55081_at
MICAL-like 1
MICAL-L1
W46406
0.02
1.5616423
|
300
60474_at
chromosome 20 open reading frame 42
C20orf42
AA469071
0.01
3.1548133
|
|
TABLE 3
|
|
Fold
|
Genbank
T-test
change
|
NO.
Probe Set ID
Gene Name
Gene Symbol
Accession #
p-value
(abs)
|
|
|
001
117_at
heat shock 70 kDa protein 6 (HSP70B$$)
HSPAB
X51757
0.03
1.7216957
|
002
1552486_s_at
lactamase, beta
LACTB
NM_171846
0.02
1.5217854
|
003
1553530_a_at
integrin, beta 1 (fibronectin receptor,
ITGB1
NM_033669
0.01
2.0436814
|
beta polypeptide, antigen CD29 includes MDF2, MSK12)
|
004
1553694_a_at
phosphoinositide-3-kinase, class 2, alpha polypeptide
PIK3C2A
NM_002645
0.03
1.6315013
|
005
1553715_s_at
hypothetical protein MGC15416
MGC15416
NM_032371
0.02
1.5123988
|
006
1554747_a_at
septin 2
02-Sep
BC033559
0.01
1.560747
|
007
1555326_a_at
ADAM metallopeptidase domain 9 (meltrin gamma)
ADAM9
AF495383
0.03
2.140922
|
008
1555060_a_at
KIAA1702 protein
KIAA1702
AK027074
0.01
1.5686767
|
009
1557987_at
PI-3-kinase-related kinase SMG-1 - like locus
LOC641298
BC042832
0.01
2.2149343
|
010
1558678_s_at
metastasis associated lung adenocarcinoma transcript 1 (non-coding
MALAT1
BE708432
0.01
2.2265985
|
RNA)
|
011
1560622_at
TPA regulated locus
TPARL
AK000203
0.03
1.5656745
|
012
1564053_a_at
YTH domain family, member 3
YTHDF3
AK093081
0.02
1.8976958
|
013
1569106_s_at
hypothetical protein FLJ10707
FLJ10707
BI087313
0.02
1.5838199
|
014
200604_s_at
protein kinase, cAMP-dependent, regulatory, type I, alpha
PRKAR1A
M18468
0.02
1.5480618
|
(tissue specific extinguisher 1)
|
015
200864_s_at
RAB11A, member RAS oncogene family
RAB11A
NM_004663
0.01
1.5156919
|
016
200927_s_at
RAB14, member RAS oncogene family
RAB14
AA919115
0.01
1.607915
|
017
201152_s_at
muscleblind-like (Drosophila)
MBNL1
N31913
0.01
1.5028459
|
018
201194_at
selenoprotein W, 1
SEPW1
NM_003009
0.01
1.8139104
|
019
201362_at
influenza virus NS1A binding protein
IVNS1ABP
AF205218
0.02
1.5876002
|
020
201363_s_at
influenza virus NS1A binding protein
IVNS1ABP
AB020657
0.01
1.6949687
|
021
201376_s_at
heterogeneous nuclear ribonucleoprotein F
HNRPF
AI591354
0.01
1.5007194
|
022
201386_s_at
DEAH (Asp-Glu-Ala-His) box polypeptide 15
DHX15
AF279891
0.01
1.7872009
|
023
201399_s_at
translocation associated membrane protein 1
TRAM1
NM_014294
0.01
1.6199075
|
024
201505_at
laminin, beta 1
LAMB1
NM_002291
0.01
2.091507
|
025
201548_s_at
Jumonji, AT rich interactive domain 1B (RBP2-like)
JARID1B
W02593
0.02
1.5838325
|
026
201549_x_at
Jumonji, AT rich interactive domain 1B (RBP2-like)
JARID1B
NM_006618
0.02
1.6096623
|
027
201559_s_at
chloride intracellular channel 4
CLIC4
AF109196
0.02
2.2302318
|
028
201578_at
podocalyxin-like
PODXL
NM_005397
0.01
2.138917
|
029
201617_x_at
caldesmon 1
CALD1
NM_004342
0.02
2.0084002
|
030
201619_at
peroxiredoxin 3
PRDX3
NM_006793
0.01
1.5513384
|
031
201646_at
scavenger receptor class B, member 2
SCARB2
AA885297
0.02
1.6010221
|
032
201647_s_at
scavenger receptor class B, member 2
SCARB2
NM_005506
0.03
1.5906466
|
033
201661_s_at
acyl-CoA synthetase long-chain family member 3
ACSL3
NM_004457
0.01
1.6001148
|
034
201678_s_at
DC12 protein
DC12
NM_020187
0.03
1.5643462
|
035
201787_at
fibulin 1
FBLN1
NM_001996
0.03
1.910708
|
036
201798_s_at
fer-1-like 3, myoferlin (C. elegans)
FER1L3
NM_013451
0.02
1.6354269
|
037
201918_at
Solute carrier family 25, member 36
SLC25A36
AI927944
0.03
1.6411883
|
038
201942_s_at
carboxypeptidase D
CPD
D85390
0.02
1.6134206
|
039
202007_at
nidogen 1
NID1
BF940043
0.03
1.784865
|
040
202143_s_at
COP9 constitutive photomorphogenic homolog subunit 8 (Arabidopsis)
COPS8
NM_006710
0.02
1.5126611
|
041
202374_s_at
RAB3 GTPase activating protein subunit 2 (non-catalytic)
RAB3GAP2
NM_012414
0.02
1.5766535
|
042
202429_s_at
protein phosphatase 3 (formerly 2B), catalytic subunit,
PPP3CA
AL353950
0.01
1.7161785
|
alpha isoform (calcineurin A alpha)
|
043
202444_s_at
SPFH domain family, member 1
SPFH1
NM_006459
0.01
1.8896967
|
044
202457_s_at
protein phosphatase 3 (formerly 2B), catalytic subunit,
PPP3CA
AA911231
0.01
1.552117
|
alpha isoform (calcineurin A alpha)
|
045
202536_at
chromatin modifying protein 2B
CHMP2B
AK002165
0.01
1.5160311
|
046
202593_s_at
membrane interacting protein of RGS16
MIR16
NM_016641
0.02
1.5102472
|
047
202627_s_at
serpin peptidase inhibitor, clade E
SERPINE1
AL574210
0.02
3.9358664
|
(nexin, plasminogen activator inhibitor type 1), member 1
|
048
202628_s_at
serpin peptidase inhibitor, clade E
SERPINE1
NM_000602
0.02
3.6850758
|
(nexin, plasminogen activator inhibitor type 1), member 1
|
049
202770_s_at
cyclin G2
CCNG2
NM_004354
0.03
1.5435082
|
050
202923_s_at
glutamate-cysteine ligase, catalytic subunit
GCLC
NM_001498
0.02
2.9063768
|
051
202946_s_at
BTB (POZ) domain containing 3
BTBD3
NM_014962
0.01
1.6240557
|
052
202955_s_at
ADP-ribosylation factor guanine nucleotide-exchange factor 1
ARFGEF1
AF084520
0.02
1.5484247
|
(brefeldin A-inhibited)
|
053
203066_at
B cell RAG associated protein
GALNAC4S-6ST
NM_014863
0.03
1.5839539
|
054
203085_s_at
transforming growth factor, beta 1 (Camurati-Engelmann disease)
TGFB1
BC000125
0.03
2.1608279
|
055
203293_s_at
lectin, mannose-binding, 1
LMAN1
NM_005570
0.02
1.9789635
|
056
203294_s_at
lectin, mannose-binding, 1
LMAN1
U09716
0.02
2.082541
|
057
203404_at
armadillo repeat containing. X-linked 2
ARMCX2
NM_014782
0.02
2.0663633
|
058
203748_x_at
RNA binding motif, single stranded interacting protein 1
RBMS1
NM_016839
0.01
1.6428717
|
059
204053_x_at
phosphatase and tensin homolog (mutated in multiple advanced
PTEN
U96180
0.02
1.7072555
|
cancers 1)
|
060
204066_s_at
centaurin, gamma 2
CENTG2
NM_014914
0.03
1.6650882
|
061
204605_at
cell growth regulator with ring finger domain 1
CGRRF1
NM_006568
0.02
1.5059351
|
062
204790_at
SMAD, mothers against DPP homolog 7 (Drosophila)
SMAD7
NM_005904
0.03
1.7849346
|
063
205180_s_at
ADAM metallopeptidase domain 8
ADAM8
NM_001109
0.03
1.8976016
|
064
205436_s_at
H2A histone family, member X
H2AFX
NM_002105
0.01
1.542324
|
065
205527_s_at
gem (nuclear organelle) associated protein 4
GEMIN4
NM_015487
0.03
1.5615736
|
066
206042_x_at
small nuclear ribonucleoprotein polypeptide N SNRPN upstream
SNRPN
NM_022804
0.02
1.6762362
|
reading frame
|
067
206113_s_at
RAB5A, member RAS oncogene family
RAB5A
NM_004162
0.02
1.7590842
|
068
206116_s_at
tropomyosin 1 (alpha)
TPM1
NM_000366
0.01
2.168161
|
069
206245_s_at
influenza virus NS1A binding protein
IVNS1ABP
NM_006469
0.01
1.5090567
|
070
207266_x_at
RNA binding motif, single stranded interacting protein 1
RBMS1
NM_016837
0.01
1.6106415
|
071
207431_s_at
degenerative spermatocyte homolog 1, lipid desaturase (Drosophila)
DEGS1
NM_003676
0.01
1.542273
|
072
207821_s_at
PTK2 protein tyrosine kinase 2
PTK2
NM_005607
0.01
1.6032615
|
073
208097_s_at
thioredoxin domain containing
TXNDC
NM_030755
0.02
1.7288516
|
074
208643_s_at
X-ray repair complementing defective repair in Chinese hamster cells 5
XRCC5
J04977
0.02
1.5489099
|
(double-strand-break rejoining; Ku autoantigen, 80 kDa)
|
075
208859_s_at
alpha thalassemia/mental retardation syndrome X-linked
ATRX
AI650257
0.02
1.6250781
|
(RAD54 homolog, S. cerevisiae)
|
076
209131_s_at
synaptosomal-associated protein, 23 kDa
SNAP23
U55936
0.01
1.8967965
|
077
209209_s_at
pleckstrin homology domain containing, family C (with FERM domain)
PLEKHC1
AW469573
0.02
2.2543647
|
member 1
|
078
209409_at
growth factor receptor-bound protein 10
GRB10
D86962
0.02
1.7913702
|
079
209647_s_at
suppressor of cytokine signaling 5
SOCS5
AW664421
0.01
1.5314134
|
080
209868_s_at
RNA binding motif, single stranded interacting protein 1
RBMS1
D28482
0.01
1.757919
|
081
210154_at
malic enzyme 2, NAD(+)-dependent, mitochondrial
ME2
M55905
0.03
1.658911
|
082
210337_s_at
ATP citrate lyase
ACLY
U18197
0.03
1.6132175
|
083
210809_s_at
periostin, osteoblast specific factor
POSTN
D13665
0.03
1.9660459
|
084
211202_s_at
Jumonji, AT rich interactive domain 1B (RBP2-like)
JARID1B
AF087481
0.03
1.6053953
|
085
211559_s_at
cyclin G2
CCNG2
L49506
0.03
2.0475583
|
086
211651_s_at
laminin, beta 1
LAMB1
M20206
0.01
2.44758
|
087
211864_s_at
fer-1-like 3, myoferlin (C. elegans)
FER1L3
AF207990
0.02
1.9618642
|
088
211981_at
collagen, type IV, alpha 1
COL4A1
NM_001845
0.03
2.0343637
|
089
211985_s_at
calmodulin 1 (phosphorylase kinase, delta)
CALM1
AI653730
0.03
1.5034102
|
090
211992_at
WNK lysine deficient protein kinase 1
WNK1
AI445745
0.02
1.5539628
|
091
212298_at
neuropilin 1
NRP1
BE620457
0.02
1.7827071
|
092
212660_at
PHD finger protein 15
PHF15
AI735639
0.02
1.7572457
|
093
212720_at
poly(A) polymerase alpha
PAPOLA
AI670847
0.02
1.6408824
|
094
212907_at
Solute carrier family 30 (zinc transporter), member 1
SLC30A1
AI972416
0.01
1.739024
|
095
213012_at
neural precursor cell expressed, developmentally down-regulated 4
NEDD4
D42055
0.02
1.6585234
|
096
213061_s_at
N-terminal asparagine amidase
NTAN1
AA643304
0.02
1.5069518
|
097
213901_x_at
RNA binding motif protein 9
RBM9
AW149379
0.02
1.5630468
|
098
214196_s_at
tripeptidyl peptidase I
TPP1
AA602532
0.02
1.8428509
|
099
214544_s_at
synaptosomal-associated protein, 23 kDa
SNAP23
NM_003825
0.02
1.8551272
|
100
214581_x_at
tumor necrosis factor receptor superfamily, member 21
TNFRSF21
BE568134
0.01
1.9035177
|
101
214701_s_at
fibronectin 1
FN1
AJ276395
0.01
2.180369
|
124
222540_s_at
hepatitis B virus x associated protein
HBXAP
BG286920
0.01
1.678279
|
125
222693_at
fibronectin type III domain containing 3B
FNDC3B
BF444916
0.02
1.5484349
|
126
223010_s_at
OCIA domain containing 1
OCIAD1
AA454649
0.01
1.638761
|
127
223110_at
KIAA1429
KIAA1429
BC003701
0.02
1.555597
|
128
223276_at
putative small membrane protein NID67
NID67
AF313413
0.02
1.8129323
|
129
223577_x_at
PRO1073 protein
PRO1073
AA827878
0.02
2.037919
|
130
223940_x_at
metastasis associated lung adenocarcinoma transcript 1 (non-coding
MALAT1
AF132202
0.01
2.7140348
|
RNA)
|
131
224567_x_at
metastasis associated lung adenocarcinoma transcript 1 (non-coding
MALAT1
BG534952
0.02
2.436764
|
RNA)
|
132
224726_at
mindbomb homolog 1 (Drosophila)
MIB1
W80418
0.03
1.5452155
|
133
224819_at
transcription elongation factor A (SII)-like 8
TCEAL8
AI743979
0.01
1.5945034
|
134
224859_at
CD276 antigen
CD276
AL360136
0.03
1.5041374
|
135
225021_at
zinc finger protein 532
ZNF532
AA861416
0.02
1.6210703
|
136
225032_at
fibronectin type III domain containing 3B
FNDC3B
AI141784
0.01
1.5388452
|
137
225168_at
FERM domain containing 4A
FRMD4A
T78406
0.01
1.8072422
|
138
225239_at
AI355441
0.02
2.2125103
|
139
225285_at
branched chain aminotransferase 1, cytosolic
BCAT1
AK025615
0.02
2.027126
|
140
225424_at
glycerol-3-phosphate acyltransferase, mitochondrial
GPAM
AB046780
0.02
1.740033
|
141
225567_at
Hypothetical LOC388114
LOC388114
BE207755
0.01
1.888815
|
142
225609_at
glutathione reductase
GSR
AI888037
0.02
2.144665
|
143
225974_at
transmembrane protein 64
TMEM64
BF732480
0.02
1.5707608
|
144
226280_at
BCL2/adenovirus E1B 19 kDa interacting protein 2
BNIP2
AA133277
0.02
1.5715192
|
145
226558_at
Full-length cDNA clone CS0DI062YC15 of Placenta Cot 25-normalized
BE856637
0.02
1.6961281
|
of Homo sapiens (human)
|
146
226675_s_at
metastasis associated, lung adenocarcinoma transcript 1 (non-coding
MALAT1
W80468
0.01
2.2176015
|
RNA)
|
147
226850_at
sulfatase modifying factor 1
SUMF1
AA683501
0.02
1.582926
|
148
227062_at
trophoblast-derived noncoding RNA
TncRNA
AU155361
0.01
3.1964853
|
149
227072_at
rotatin
RTTN
BG167480
0.02
1.6342819
|
150
227080_at
zinc finger protein 697
ZNF697
AW003092
0.01
2.047982
|
151
227257_s_at
chromosome 10 open reading frame 46
C10orf46
AW973842
0.02
1.8308182
|
152
227456_s_at
chromosome 6 open reading frame 136
C6orf136
BF224092
0.02
1.5313978
|
153
229586_at
chromodomain helicase DNA binding protein 9
CHD9
AW300405
0.01
1.6146306
|
154
229606_at
Protein phosphatase 3 (formerly 2B), catalytic subunit, alpha isoform
PPP3CA
AI827550
0.02
1.5514666
|
(calcineurin A alpha)
|
155
229982_at
hypothetical protein FLJ21924
FLJ21924
AW195525
0.03
1.5703845
|
156
231735_s_at
PRO1073 protein
PRO1073
NM_014086
0.02
2.0209107
|
157
231823_s_at
KIAA1295
KIAA1295
BG054798
0.03
1.527874
|
158
234989_at
trophoblast-derived noncoding RNA
TncRNA
AV699657
0.02
2.0119648
|
159
235138_at
Pumilio homolog 2 (Drosophila)
PUM2
AA565051
0.01
1.7716993
|
160
235879_at
Muscleblind-like (Drosophila)
MBNL1
AI697540
0.01
2.2558458
|
161
236841_at
CXYorf1-related protein
FLJ25222
BE464132
0.01
1.7994804
|
162
238549_at
core-binding factor, runt domain, alpha subunit 2; translocated to, 2
CBFA2T2
AI420611
0.01
1.928193
|
163
239742_at
Tubby like protein 4
TULP4
H15278
0.03
1.5802637
|
164
242121_at
AW973232
0.03
1.7029374
|
165
243768_at
SUMO1/sentrin specific peptidase 6
SENP6
AA026388
0.01
2.2681193
|
166
244804_at
Sequestosome 1
SQSTM1
AW293441
0.01
1.5338039
|
|
In Tables 1, 2 and 3, gene name denotes a name of a gene, gene symbol denotes a symbol representing a gene, and Genbank Accession # denotes a number accessing Genbank which is a database that the public can access. T-test p value is obtained by statistically analyzing the degree of difference between an average expression level in a patient with lung cancer recurrence and an average expression level in a patient without lung cancer recurrence after lung cancer tissue removal operation.
Here, an expression level was calculated by Affymetrix GeneChip Operating Software (GCOS) Version 1.3 after a hybridization analysis using a microarray on which a probe is immobilized. Fold change (abs) indicates a ratio between an average expression level in a patient with lung cancer recurrence and an average expression level in a patient without lung cancer recurrence after lung cancer tissue removal operation in a hybridization analysis using a microarray on which a probe is immobilized.
As shown in Tables 1, 2 and 3, expression values of at least one marker gene selected from the group consisting of marker genes of Genbank Accession No. shown in Tables 1, 2 and 3 showed statistically meaningful differences such that both T-test p values of the patient with lung cancer recurrence and the patient without lung cancer recurrence were less than 0.05. Therefore, at least one marker gene selected from the group consisting of marker genes of Genbank Accession No. shown in Tables 1, 2 and 3 can be used as a marker gene that can predict whether lung cancer is recurred afterwards with respect to the patients with a lung cancer removal operation. In addition, at least one marker gene selected from the group consisting of marker genes of Genbank Accession No. shown in Tables 1, 2 and 3 had showed that all the ratios of an expression average of the patients with lung cancer recurrence to an expression average of the patients without lung cancer recurrence was at least 1.5:1. Accordingly, it was confirmed that the expression of the marker gene was significantly increased in the patients with lung cancer recurrence.
DETAILED DESCRIPTION OF THE INVENTION
Hereinafter, the present invention will be described more specifically with reference to the following Examples. The following Examples are for illustrative purposes and are not intended to limit the scope of the invention.
EXAMPLE
Example 1
Selection of Marker Gene Related to Lung Cancer Recurrence
Primary lung cancer tissue having a tumor size of less than 3 cm and without lymph node metastase (that is, N0M0T1 stage) was collected. Total RNA was then immediately isolated from the collected lung cancer tissue. All the collected tumor tissue was lightly dyed with hematoxylin in order to improve visualization prior to RNA extraction. Each finely cut sample comprised at least 90% of tumor cells.
To avoid a necrotic region, one or two pieces of tumor tissue having a size of 5 mm×5 mm from the edge of tumor mass was immediately stored at −80□.
The finely cut tumor tissue was added to 1 ml of a Trizol reagent (Life Technologies, Rockville, Md.), and immediately homogenized by vortexing. Total RNA was isolated according to Trizol reagent protocol. The quality of the isolated total RNA was analyzed by electrophoresis using 1% agarose gel comprising 0.6 M formamide and ethidium bromide. An amount of total RNA was analyzed using a Nanodrop spectrometer (Nanodrop Technologies, Rockland, Del.).
The quality and amount of the isolated total RNA were confirmed to be excellent, and a reverse transcription reaction was performed using the RNA as a template and oligo dT as a primer to obtain cDNA. The obtained cDNA was used as a template that synthesizes cRNA through an in vitro transcription reaction. At this time, cRNA synthesized by adding UTP modified with biotin to a reaction solution was labeled with biotin. Next, the synthesized biotin-labeled cRNA was reacted with a hydroxyl radical to be fragmentized with a size of 50-200 bp. 10 μg of the fragmentized cRNA sample was injected onto an Affymetrix GeneChip array (human 133 plus ver 2) and hybridized at 45□ for 16 hours. The hybridization mixture was then removed and the microarrays were washed, stained with phycoerythrin-labeled Streptavidin, washed, incubated with biotinylated anti-streptavidin, and then restained with phycoerythrin-labeled Streptavidin to amplify the signals. Arrays were scanned using the GeneChip Scanner 3000 7G scanner (Affymetrix), controlled by Affymetrix GeneChip Operating System (GCOS) software. The Affymetrix Microarray Suite version 5 (MAS5) algorithm were utilized to analyze the hybridization intensity data from the microarrays and calculate a set of matrixes that describe probe set performance.
The obtained data was analyzed using an ArrayAssist™ (Stratagene, Inc., San Diego, USA) program. Data preprocessing was performed using a GCRMA (log 2 transformation) method that is a normalization method of multi-microarray level, in which fluorescence intensity values with respect to total microarrays used in analysis were substituted with log 2, and a fluorescence intensity average with respect to the total microarrays was adjusted taking into consideration of a GC amount of a nucleic acid sequence. Comparison between groups was performed under conditions of unpaired t-test, permutation=100, corrected p-value, Number of False Discovery Rate (NO/FDR). Data filtering was performed by selecting only data that satisfied an expression level (recurrence and non-recurrence, group average)>5 and fold change≧1.5. A count for each probeset_id was defined as the number of probe sets that showed a gene expression difference that satisfies the filtering standard in ADC, SQC, or in the recurrence group and non-recurrence group regardless of cell types.
As a result of analysis, the number of markers selected as positive expression with respect to adenocarcinoma (ADC) and squamous cell carcinoma (SQC) are shown in Table 4 below.
TABLE 4
|
|
total
squamous cell
|
lung cancer tissue
adenocarcinoma
carcinoma
|
|
|
number of probe
166
300
166
|
|
Data related to expression of each gene that was obtained by the measurement of fluorescence intensity was obtained. To confirm correlation between the collected data related to expression of gene and lung cancer recurrence, patients with a lung cancer removal operation were monitored for five years to confirm lung cancer recurrence or non-recurrence. In the case of patients with lung cancer recurrence within one year after a lung cancer removal operation, they were grouped into a lung cancer recurrence group. In the case of patients without lung cancer recurrence even after three years after a lung cancer removal operation, they were grouped into a non-recurrence group. Data with respect to the obtained recurrence group and non-recurrence group among patients with a lung cancer removal operation was obtained.
Next, correlation between an expression pattern of each gene which was analyzed during the lung cancer removal operation, and the recurrence and non-recurrence groups that were subsequently obtained by monitoring the patients with a lung cancer removal operation was analyzed. The results are shown in Tables 1, 2 and 3.
Table 1 represents the results in which the gene expression pattern of the lung cancer cell after lung cancer tissue removal operation is analyzed through hybridization with a probe on a microarray, and a marker gene is selected, the marker gene being determined to have a difference in an expression level between a patient with lung cancer recurrence within one year and a patient without lung cancer recurrence even after three years. The total number of patients was 60. Among them, the number of patients with lung cancer recurrence within one year after lung cancer tissue removal operation was 19, and the number of patients without lung cancer recurrent even after three years was 41.
Table 2 represents the results in which the gene expression pattern of the lung cancer cell which was classified into adenocarcinoma after lung cancer tissue removal operation is analyzed through hybridization with a probe on a microarray, and a marker gene is selected, the marker gene being determined to have a difference in an expression level between a patient with lung cancer recurrence within one year and a patient without lung cancer recurrence even after three years. A total number of adenocarcinoma patients was 23. Among them, the number of patients with lung cancer recurrence within one year after lung cancer tissue removal operation was 8, and the number of patients without lung cancer recurrent even after three years was 15.
Table 3 represents the results in which the gene expression pattern of the lung cancer cell which was classified into squamous cell carcinoma after lung cancer tissue removal operation is analyzed through hybridization with a probe on a microarray, and a marker gene is selected, the marker gene being determined to have a difference in an expression level between a patient with lung cancer recurrence within one year and a patient without lung cancer recurrence even after three years. The total number of squamous cell carcinoma patients was 37. Among them, the number of patients with lung cancer recurrence within one year after lung cancer tissue removal operation was 11, and the number of patients without lung cancer recurrent even after three years was 26.
As shown in Tables 1, 2 and 3, expression values of at least one marker gene selected from the group consisting of marker genes of Genbank Accession No. shown in Tables 1, 2 and 3 showed statistically meaningful differences such that both T-test p values of the patient with lung cancer recurrence and the patient without lung cancer recurrence were less than 0.05. Therefore, at least one marker gene selected from the group consisting of marker genes of Genbank Accession No. shown in Tables 1, 2 and 3 can be used as a marker gene that can predict whether lung cancer is likely to recur with respect to the patients that have had a lung cancer removal operation. In addition, at least one marker gene selected from the group consisting of marker genes of Genbank Accession No. shown in Tables 1, 2 and 3 showed that all the ratios of an expression average of the patients with lung cancer recurrence to an expression average of the patients without lung cancer recurrence were at least 1.5:1. Accordingly, it was confirmed that the expression of the marker gene was significantly increased in the patients with lung cancer recurrence.
The relationships between lung cancer recurrence in patients after lung cancer removal operation and conditions of the patients such as age, sex, smoking, cell type, pstage, and tumor size were analyzed, and the results are shown in Tables 5, 6 and 7.
TABLE 5
|
|
variation
statistical analysis method
result
|
|
sex
chi-square test
no difference: p value = 0.552
|
age
2-sample t-test
no difference: p value = 0.559
|
smoking
chi-square test
no difference: p value = 0.813
|
cell type
chi-square test
no difference: p value = 0.682
|
pstage
Fisher's exact test
no difference: p value = 0.305
|
tumor size
2-sample t-test
difference: p value = 0.039
|
metastasis
—
no metastasis
|
|
Table 5 shows results of analyzing 60 patients without classifying them according to cell types of lung cancer. Among 60 patients, the number of patients with lung cancer recurrence was 19, and the number of patients without lung cancer recurrence was 41. As shown in Table 5, the clinical indexes from the all patients looked no statistically meaningful difference in the recurrence group and the non-recurrence group. That is, the analyzed result can be regarded as a gene list that represents statistically meaningful difference in expression only with respect to the recurrence.
TABLE 6
|
|
variation
statistical analysis method
result
|
|
sex
Fisher's exact test
no difference: p value = 1.000
|
age
2-sample t-test
no difference: p value = 0.618
|
smoking
chi-square test
no difference: p value = 0.6570
|
cell type
—
adenocarcinoma (ADC)
|
pstage
Fisher's exact test
no difference: p value = 0.085
|
tumor size
2-sample t-test
no difference: p value = 0.051
|
metastasis
—
no metastasis
|
|
Table 6 shows results of analyzing 23 patients having adenocarcinoma when they are classified according to cell types of lung cancer. Among 23 patients, the number of patients with lung cancer recurrence was 8, and the number of patients without lung cancer recurrence was 15. As shown in Table 6, clinical information except the recurrence and tumor size which may induce confounding in other analysis may not have any statistically meaningful difference in the recurrence group and the non-recurrence group. That is, the analyzed result can be regarded as a gene list that represents statistically meaningful difference in expression only with respect to the recurrence.
TABLE 7
|
|
variation
statistical analysis method
result
|
|
sex
—
man
|
age
2-sample t-test
no difference: p value = 0.328
|
smoking
chi-square test
no difference: p value = 1.000
|
cell type
—
squamous cell carcinoma
|
(SQC)
|
pstage
Fisher's exact test
no difference: p value = 1.000
|
tumor size
2-sample t-test
no difference: p value = 0.417
|
metastasis
—
no metastasis
|
|
Table 7 shows results of analyzing 37 patients having squamous cell carcinoma when they are classified according to cell types of lung cancer. Among 23 patients, the number of patients with lung cancer recurrence was 11, and the number of patients without lung cancer recurrence was 26. As shown in Table 7, clinical information except the recurrence and tumor size which may induce confounding in other analysis may not have any statistically meaningful difference in the recurrence group and the non-recurrence group. That is, the analyzed result can be regarded as a gene list that represents statistically meaningful difference in expression only with respect to the recurrence.
Example 2
Prediction of Risk of Lung Cancer Recurrence Using Statistical Model
Based on the expression level of marker genes collected from the patients with lung cancer recurrence and non-recurrence which were obtained in Example 1, it was confirmed whether a risk of lung cancer recurrence could be predicted using a statistical analysis model.
In the analysis, a portion of each of data obtained with respect to total lung cancer tissue, adenocarcinoma and squamous cell carcinoma was used as a learning set to establish a basis on the prediction accuracy of the statistical model, the other portion of the data was used to identify whether the establish prediction accuracy is actually accurate using the leaning set
Data of learning sets and test sets with respect to the total lung cancer tissue, adenocarcinoma and squamous cell carcinoma are shown in Tables 8, 9 and 10.
TABLE 8
|
|
total lung cancer tissue
non-recurrence
recurrence
total
|
|
|
learning set
28
15
43
|
test set
13
4
17
|
total
41
19
60
|
|
TABLE 9
|
|
adenocarcinoma
non-recurrence
recurrence
total
|
|
|
learning set
9
6
15
|
test set
6
2
8
|
total
16
8
23
|
|
TABLE 10
|
|
squamous cell
|
carcinoma
non-recurrence
recurrence
total
|
|
|
learning set
17
7
24
|
test set
9
4
13
|
total
26
11
37
|
|
Results of predicting the test set with respect to the lung cancer tissue, adenocarcinoma and squamous cell carcinoma using a QDA prediction model are shown in Tables 11, 12 and 13 below. As shown in Tables 11, 12 and 13, the overall accuracy was at least 76.4%.
TABLE 11
|
|
Predicted results of the total lung cancer tissue using a QDA
|
prediction model
|
predicted class
|
classification
non-recurrence
recurrence
total
|
|
true class
non-recurrence
10
1
11
|
recurrence
3
3
6
|
overall accuracy
76.4%
|
|
The overall accuracy in Table 11 is a percentage of predicted class which corresponds to true class per total sample. That is, the overall accuracy is (17−4)×100/17=76.4%. The total is calculated in the same manner described above.
TABLE 12
|
|
Predicted results of adenocarcinoma tissue using
|
a QDA prediction model
|
predicted class
|
classification
non-recurrence
recurrence
total
|
|
true class
non-recurrence
6
0
6
|
recurrence
0
2
2
|
overall accuracy
100%
|
|
TABLE 13
|
|
Predicted results of squamous cell carcinoma
|
tissue using a QDA prediction model
|
predicted class
|
classification
non-recurrence
recurrence
total
|
|
true class
non-recurrence
9
2
11
|
recurrence
0
2
2
|
overall accuracy
84.6%
|
|
Results of predicting the test set with respect to the lung cancer tissue, adenocarcinoma and squamous cell carcinoma using a Linear Discrimination Analysis (LDA) prediction model are shown in Tables 14, 15 and 16 below. As shown in Tables 14, 15 and 16, the overall accuracy was at least 76.4%.
TABLE 14
|
|
Predicted results of the total lung cancer tissue using a LDA
|
prediction model
|
predicted class
|
classification
non-recurrence
recurrence
total
|
|
true class
non-recurrence
10
1
11
|
recurrence
3
3
6
|
overall accuracy
76.4%
|
|
TABLE 15
|
|
Predicted results of adenocarcinoma tissue using a LDA prediction
|
model
|
predicted class
|
classification
non-recurrence
recurrence
total
|
|
true class
non-recurrence
6
0
6
|
recurrence
0
2
2
|
overall accuracy
100%
|
|
TABLE 16
|
|
Predicted results of squamous cell carcinoma tissue using a LDA
|
prediction model
|
predicted class
|
classification
non-recurrence
recurrence
total
|
|
true class
non-recurrence
9
1
10
|
recurrence
0
3
3
|
overall accuracy
92.3%
|
|
Results of predicting the test set with respect to the lung cancer tissue, adenocarcinoma and squamous cell carcinoma using a Neural network prediction model are shown in Tables 17, 18 and 19 below. As shown in Tables 17, 18 and 19, the overall accuracy was at least 59.46%.
TABLE 17
|
|
Predicted results of the total lung cancer tissue using a Neural
|
network prediction model
|
predicted class
|
classification
non-recurrence
recurrence
total
|
|
true class
non-recurrence
40
1
41
|
recurrence
18
1
19
|
overall accuracy
68.33%
|
|
TABLE 18
|
|
Predicted results of adenocarcinoma tissue using a Neural
|
network prediction model
|
predicted class
|
classification
non-recurrence
recurrence
total
|
|
true class
non-recurrence
14
1
15
|
recurrence
1
7
8
|
overall accuracy
91.3%
|
|
TABLE 19
|
|
Predicted results of squamous cell carcinoma tissue using
|
a Neural network prediction model
|
predicted class
|
classification
non-recurrence
recurrence
total
|
|
true class
non-recurrence
20
6
26
|
recurrence
9
2
11
|
overall accuracy
59.46%
|
|
Results of predicting the test set with respect to the lung cancer tissue, adenocarcinoma and squamous cell carcinoma using a Decision tree prediction model are shown in Tables 20, 21 and 22 below. As shown in Tables 20, 21 and 22, the overall accuracy was at least 61.67%.
TABLE 20
|
|
Predicted results of the total lung cancer tissue using a Decision
|
tree prediction model
|
predicted class
|
classification
non-recurrence
recurrence
total
|
|
true class
non-recurrence
35
6
41
|
recurrence
17
2
19
|
overall accuracy
61.67%
|
|
TABLE 21
|
|
Predicted results of adenocarcinoma tissue using a Decision tree
|
prediction model
|
predicted class
|
classification
non-recurrence
recurrence
total
|
|
true class
non-recurrence
15
0
15
|
recurrence
8
0
8
|
overall accuracy
65.22%
|
|
TABLE 22
|
|
Predicted results of squamous cell carcinoma tissue using a
|
Decision tree prediction model
|
predicted class
|
classification
non-recurrence
recurrence
total
|
|
true class
non-recurrence
25
1
26
|
recurrence
2
9
11
|
overall accuracy
91.89%
|
|
Results of predicting the test set with respect to the lung cancer tissue, adenocarcinoma and squamous cell carcinoma using a Support vector machine prediction model are shown in Tables 23, 24 and 25 below. As shown in Tables 23, 24 and 25, the overall accuracy was at least 65%.
TABLE 23
|
|
Predicted results of the total lung cancer tissue using a Support
|
vector machine prediction model
|
predicted class
|
classification
non-recurrence
recurrence
total
|
|
true class
non-recurrence
37
4
41
|
recurrence
17
2
19
|
overall accuracy
65%
|
|
TABLE 24
|
|
Predicted results of adenocarcinoma tissue using a Support vector
|
machine prediction model
|
predicted class
|
classification
non-recurrence
recurrence
total
|
|
true class
non-recurrence
15
0
15
|
recurrence
1
7
8
|
overall accuracy
95.65%
|
|
TABLE 25
|
|
Predicted results of squamous cell carcinoma tissue using a
|
Support vector machine prediction model
|
predicted class
|
classification
non-recurrence
recurrence
total
|
|
true class
non-recurrence
24
2
26
|
recurrence
1
10
11
|
overall accuracy
91.89%
|
|
Results of predicting the test set with respect to the lung cancer tissue, adenocarcinoma and squamous cell carcinoma using a Naive Bayes prediction model are shown in Tables 26, 27 and 28 below. As shown in Tables 26, 27 and 28, the overall accuracy was at least 58.33%.
TABLE 26
|
|
Predicted results of the total lung cancer tissue using a Naive Bayes
|
prediction model
|
predicted class
|
classification
non-recurrence
recurrence
total
|
|
true class
non-recurrence
26
15
41
|
recurrence
10
9
19
|
overall accuracy
58.33%
|
|
TABLE 27
|
|
Predicted results of adenocarcinoma tissue using a Naive Bayes
|
prediction model
|
predicted class
|
classification
non-recurrence
recurrence
total
|
|
true class
non-recurrence
15
0
15
|
recurrence
1
7
8
|
overall accuracy
95.65%
|
|
TABLE 28
|
|
Predicted results of squamous cell carcinoma tissue using a Naive
|
Bayes prediction model
|
predicted class
|
classification
non-recurrence
recurrence
total
|
|
true class
non-recurrence
24
2
26
|
recurrence
1
10
11
|
overall accuracy
91.89%
|
|
The prediction models utilized in Examples of the present invention could have been easily understood by one of ordinary skill in the art (SAS Language: Reference, Version 6, First Edition by the SAS Institute.).
According to the method of predicting risk of lung cancer recurrence in a lung cancer patient or after a patient has lung cancer treatment according to the present invention, the risk of lung cancer recurrence in a lung cancer patient after a lung cancer removal operation can be predicted with high accuracy.
According to the method of preparing a report on the risk of lung cancer recurrence in a lung cancer patient or after a patient has lung cancer treatment according to the present invention, the report can be prepared to include results predicting the risk of lung cancer recurrence in a lung cancer patient after a lung cancer removal operation with high accuracy.
The report on the risk of lung cancer recurrence in a lung cancer patient or after the patient has lung cancer treatment according to the present invention includes highly accurate results predicting the risk of lung cancer recurrence in a lung cancer patient after a lung cancer removal operation.
According to the composition, kit and microarray for diagnosing the risk of lung cancer recurrence in a lung cancer patient or after a patient has lung cancer treatment according to the present invention, diagnosis efficiency of risk of lung cancer recurrence of a lung cancer patient after a lung cancer treatment can be increased.
While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it will be understood by those of ordinary skill in the art that various changes in form and details may be made therein without departing from the spirit and scope of the present invention as defined by the following claims.