Method of Preferentially Inducing the Biosynthesis of Interferon

BACKGROUND

Certain compounds have been found to be useful as immune response modifiers (IRMs), rendering them useful in the treatment of a variety of disorders. However, there continues to be interest in and a need for compounds that have the ability to modulate the immune response, by induction of cytokine biosynthesis or other means.

SUMMARY

The present invention provides a method of preferentially inducing the biosynthesis of interferon (α) (IFN-α) in an animal comprising administering an effective amount of a compound of Formulas I, II, and/or III:

wherein R_A, R_B, R₁, G₁, G₂, and n are as defined below.

It has now surprisingly been discovered that the amount of TNF-α induced by the 2-(hydroxyalkyl) substituted compounds of Formula I is substantially less than the amount of TNF-α induced by closely related analogs having an alkyl or alkyl ether substituent at the 2-position and that the compounds of Formula I, which can be administered as Formula I, Formula II, and/or Formula III, and/or a pharmaceutically acceptable salt thereof, can still retain the ability to induce the biosynthesis of IFN-α. See, for example, FIGS. 1-8 below. The reduction in the amount of TNF-α induced is seen over a broad range of test concentrations. In some embodiments the amount of TNF-α induced by the compounds of Formulas I, II, and/or III is at least two-fold less than the amount of TNF-α induced by analogs having an alkyl or alkyl ether substituent at the 2-position. In other embodiments the amount of TNF-α induced by the compounds of Formulas I, II, and/or III is at least three-fold less than the amount of TNF-α induced by analogs having an alkyl or alkyl ether substituent at the 2-position. In still other embodiments the amount of TNF-α induced by the compounds of Formulas I, II, and/or III is at least four-fold less than the amount of TNF-α induced by analogs having an alkyl or alkyl ether substituent at the 2-position.

The compounds or salts of Formulas I, II, and III are especially useful as immune response modifiers due to their ability to preferentially induce interferon-α, thus providing a benefit over compounds that also induce pro-inflammatory cytokines (e.g. TNF-α) or that induce pro-inflammatory cytokines at higher levels.

A compound is said to preferentially induce IFN-α if, when tested according to the test methods described herein, the effective minimum concentration for IFN-α induction is less than the effective minimum concentration for TNF-α induction. In some embodiments, the effective minimum concentration for IFN-α induction is at least 3-fold less than the effective minimum concentration for TNF-α induction. In some embodiments, the effective minimum concentration for IFN-α induction is at least 6-fold less than the effective minimum concentration for TNF-α induction. In other embodiments, the effective minimum concentration for IFN-α induction is at least 9-fold less than the effective minimum concentration for TNF-α induction. In some embodiments, when tested according to the test methods described herein, the amount TNF-α induced by compounds of Formulas I, II, and/or III is at or below the background level of TNF-α in the test method.

The invention further provides a method of preferentially inducing the biosynthesis of IFN-α in an animal wherein an effective amount of the compound or salt of Formulas I, II, and/or III (or any one of the embodiments described herein) is administered as a pharmaceutical composition comprising a therapeutically effective amount of a compound or salt of Formulas I, II, and/or III (or any one of the embodiments described herein) and a pharmaceutically acceptable carrier.

The invention further provides a method of treating a viral infection or disease and/or treating a neoplastic disease in an animal comprising preferentially inducing the biosynthesis of IFN-α in the animal by administering an effective amount of a compound or salt of Formulas I, II, and/or III (or any one of the embodiments described herein) or a pharmaceutical composition containing an effective amount of a compound or salt of Formulas I, II, and/or III (or any one of the embodiments described herein) to the animal.

In addition, methods of synthesizing compounds of Formulas I, II, and III and intermediates useful in the synthesis of these compounds are provided.

As used herein, “a,” “an,” “the,” “at least one,” and “one or more” are used interchangeably.

The terms “comprises” and variations thereof do not have a limiting meaning where these terms appear in the description and claims.

The above summary of the present invention is not intended to describe each disclosed embodiment or every implementation of the present invention. The description that follows more particularly exemplifies illustrative embodiments. In several places throughout the description, guidance is provided through lists of examples, which examples can be used in various combinations. In each instance, the recited list serves only as a representative group and should not be interpreted as an exclusive list.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1 shows the IFN-α dose response curves (corresponding to values shown in Table 7 below) for Example 6, Analog 2, Analog 3, and Analog 5.

FIG. 2 shows the TNF-α dose response curves (corresponding to values shown in Table 7 below) for Example 6, Analog 2, Analog 3, and Analog 5.

FIG. 3 shows the IFN-α dose response curves (corresponding to values shown in Table 7 below) for Example 7, Analog 1, Analog 2, and Analog 4.

FIG. 4 shows the TNF-α dose response curves (corresponding to values shown in Table 7 below) for Example 7, Analog 1, Analog 2, and Analog 4.

FIG. 5 shows the IFN-α dose response curves (corresponding to values shown in Table 8 below) for Example 148, Example 149, Analog 6, and Analog 7.

FIG. 6 shows the TNF-α dose response curves (corresponding to values shown in Table 8 below) for Example 148, Example 149, Analog 6, and Analog 7.

FIG. 7 shows the IFN-α dose response curves (corresponding to values shown in Table 9 below) for Example 163, Analog 8, Analog 9, and Analog 10.

FIG. 8 shows the TNF-α dose response curves (corresponding to values shown in Table 9 below) for Example 163, Analog 8, Analog 9, and Analog 10.

DETAILED DESCRIPTION OF ILLUSTRATIVE EMBODIMENTS OF THE INVENTION

The present invention provides a method of preferentially inducing the biosynthesis of IFN-α in an animal comprising administering an effective amount of a compound of Formulas I, II, and/or III:

wherein R_A, R_B, R₁, G₁, G₂, and n are as defined below; and pharmaceutically acceptable salts thereof.

In one embodiment, the present invention provides a method of preferentially inducing the biosynthesis of IFN-α in an animal comprising administering an effective amount of a compound of the following Formula I:

wherein:

n is 1 or 2;

R_Aand R_Bare each independently selected from the group consisting of:

- hydrogen,
- halogen,
- alkyl,
- alkenyl,
- alkoxy,
- alkylthio and
- —N(R₉)₂;

or when taken together, R_Aand R_Bform a fused aryl ring or heteroaryl ring containing one heteroatom selected from the group consisting of N and S wherein the aryl or heteroaryl ring is unsubstituted or substituted by one or more R groups, or substituted by one R₃group, or substituted by one R₃group and one R group;

or when taken together, R_Aand R_Bform a fused 5 to 7 membered saturated ring, optionally containing one heteroatom selected from the group consisting of N and S, and unsubstituted or substituted by one or more R groups;

R is selected from the group consisting of:

- halogen,
- hydroxy,
- alkyl,
- alkenyl,
- haloalkyl,
- alkoxy,
- alkylthio, and
- —N(R₉)₂;

R₁is selected from the group consisting of:

- -R₄,
- -X-R₄,
- -X-Y-R₄,
- -X-Y-X-Y-R₄, and
- -X-R₅;

R₃is selected from the group consisting of:

- -Z-R₄,
- -Z-X-R₄,
- -Z-X-Y-R₄,
- -Z-X-Y-X-Y-R₄, and
- -Z-X-R₅;

X is selected from the group consisting of alkylene, alkenylene, alkynylene, arylene, heteroarylene, and heterocyclylene wherein the alkylene, alkenylene, and alkynylene groups can be optionally interrupted or terminated by arylene, heteroarylene or heterocyclylene and optionally interrupted by one or more —O— groups;

Y is selected from the group consisting of:

Z is a bond or —O—;

R₄is selected from the group consisting of hydrogen, alkyl, alkenyl, alkynyl, aryl, arylalkylenyl, aryloxyalkylenyl, alkylarylenyl, heteroaryl, heteroarylalkylenyl, heteroaryloxyalkylenyl, alkylheteroarylenyl, and heterocyclyl wherein the alkyl, alkenyl, alkynyl, aryl, arylalkylenyl, aryloxyalkylenyl, alkylarylenyl, heteroaryl, heteroarylalkylenyl, heteroaryloxyalkylenyl, alkylheteroarylenyl, and heterocyclyl groups can be unsubstituted or substituted by one or more substituents independently selected from the group consisting of alkyl, alkoxy, hydroxyalkyl, haloalkyl, haloalkoxy, halogen, nitro, hydroxy, mercapto, cyano, aryl, aryloxy, arylalkyleneoxy, heteroaryl, heteroaryloxy, heteroarylalkyleneoxy, heterocyclyl, amino, alkylamino, dialkylamino, (dialkylamino)alkyleneoxy, and in the case of alkyl, alkenyl, alkynyl, and heterocyclyl, oxo;

R₅is selected from the group consisting of

R₆is selected from the group consisting of ═O and ═S;

R₇is C_2-7alkylene;

R₈is selected from the group consisting of hydrogen, alkyl, alkoxyalkylenyl, hydroxyalkylenyl, arylalkylenyl, and heteroarylalkylenyl;

R₉is selected from the group consisting of hydrogen and alkyl;

R₁₀is C_3-8alkylene;

A is selected from the group consisting of —O—, —C(O)—, —CH₂—, —S(O)_0-2—, and —N(Q-R₄)—;

A′ is selected from the group consisting of —O—, —S(O)_0-2—, —N(-Q-R₄)—, and —CH₂—;

Q is selected from the group consisting of a bond, —C(R₆)—, —C(R₆)—C(R₆)—, —S(O)₂—, —C(R₆)—N(R₈)—W—, —S(O)₂—N(R₈)—, —C(R₆)—O—, —C(R₆)—S—, and —C(R₆)—N(OR₉)—;

V is selected from the group consisting of —C(R₆)—, —O—C(R₆)—, —N(R₈)—C(R₆)—, and —S(O)₂—;

W is selected from the group consisting of a bond, —C(O)—, and —S(O)₂—; and

a and b are independently integers from 1 to 6 with the proviso that a+b is ≦7;

or a pharmaceutically acceptable salt thereof to the animal.

In another embodiment, the present invention provides a method of preferentially inducing the biosynthesis of IFN-α in an animal comprising administering an effective amount of a compound of the following Formula II, which is a prodrug:

wherein:

G₁is selected from the group consisting of:

- —C(O)—R′,
- α-aminoacyl,
- α-aminoacyl-α-aminoacyl,
- C(O)—O—R′,
- —C(O)—N(R″)R′,
- —C(═NY′)—R′,
- —CH(OH)—C(O)—OY′,
- —CH(OC_1-4alkyl)Y₀,
- —CH₂Y₁, and
- —CH(CH₃)Y₁;

R′ and R″ are independently selected from the group consisting of C_1-10alkyl, C_3-7cycloalkyl, phenyl, benzyl, and 2-phenylethyl, each of which may be unsubstituted or substituted by one or more substituents independently selected from the group consisting of halogen, hydroxy, nitro, cyano, carboxy, C_1-6alkyl, C_1-4alkoxy, aryl, heteroaryl, aryl-C_1-4alkylenyl, heteroaryl-C_1-4alkylenyl, halo-C_1-4alkylenyl, halo-C_1-4alkoxy, —O—C(O)—CH₃, —C(O)—O—CH₃, —C(O)—NH₂, —O—CH₂—C(O)—NH₂, —NH₂, and —S(O)₂—NH₂, with the proviso that R″ can also be hydrogen;

α-aminoacyl is an α-aminoacyl group derived from an α-amino acid selected from the group consisting of racemic, D-, and L-amino acids;

Y′ is selected from the group consisting of hydrogen, C_1-6alkyl, and benzyl;

Y₀is selected from the group consisting of C_1-6alkyl, carboxy-C_1-6alkylenyl, amino-C_1-4alkylenyl, mono-N—C_1-6alkylamino-C_1-4alkylenyl, and di-N,N—C_1-6alkylamino-C_1-4alkylenyl;

Y₁is selected from the group consisting of mono-N—C_1-6alkylamino, di-N,N—C_1-6alkylamino, morpholin-4-yl, piperidin-1-yl, pyrrolidin-1-yl, and 4-C_1-4alkylpiperazin-1-yl;

n is 1 or 2;

R_Aand R_Bare each independently selected from the group consisting of:

- hydrogen,
- halogen,
- alkyl,
- alkenyl,
- alkoxy,
- alkylthio and
- —N(R₉)₂;

R is selected from the group consisting of:

- halogen,
- hydroxy,
- alkyl,
- alkenyl,
- haloalkyl,
- alkoxy,
- alkylthio, and
- —N(R₉)₂;

R₁is selected from the group consisting of:

- -R₄,
- -X-R₄,
- -X-Y-R₄,
- -X-Y-X-Y-R₄, and
- -X-R₅;

R₃is selected from the group consisting of:

- -Z-R₄,
- -Z-X-R₄,
- -Z-X-Y-R₄,
- -Z-X-Y-X-Y-R₄, and
- -Z-X-R₅;

Y is selected from the group consisting of:

Z is a bond or —O—;

R₅is selected from the group consisting of

R₆is selected from the group consisting of ═O and ═S;

R₇is C_2-7alkylene;

R₈is selected from the group consisting of hydrogen, alkyl, alkoxyalkylenyl, hydroxyalkylenyl, arylalkylenyl, and heteroarylalkylenyl;

R₉is selected from the group consisting of hydrogen and alkyl;

R₁₀is C_3-8alkylene;

A is selected from the group consisting of —O—, —C(O)—, —CH₂—, —S(O)_0-2—, and —N(Q-R₄)—;

A′ is selected from the group consisting of —O—, —S(O)_0-2—, —N(-Q-R₄)—, and —CH₂—;

V is selected from the group consisting of —C(R₆)—, —O—C(R₆)—, —N(R₈)—C(R₆)—, and —S(O)₂—;

W is selected from the group consisting of a bond, —C(O)—, and —S(O)₂—; and

a and b are independently integers from 1 to 6 with the proviso that a+b is ≦7;

or a pharmaceutically acceptable salt thereof to the animal.

wherein:

G₂is selected from the group consisting of:

- —X₂—C(O)—R′,
- α-aminoacyl,
- α-aminoacyl-α-aminoacyl,
- —X₂—C(O)—O—R′, and
- —C(O)—N(R″)R′;

X₂is selected from the group consisting of a bond; —CH₂—O—; —CH(CH₃)—O—, —C(CH₃)₂—O—; and, in the case of —X₂—C(O)—O—R′, —CH₂—NH—;

α-aminoacyl is an α-aminoacyl group derived from an α-amino acid selected from the group consisting of racemic, D-, and L-amino acids;

n is 1 or 2;

R_Aand R_Bare each independently selected from the group consisting of:

- hydrogen,
- halogen,
- alkyl,
- alkenyl,
- alkoxy,
- alkylthio and
- —N(R₉)₂;

R is selected from the group consisting of:

- halogen,
- hydroxy,
- alkyl,
- alkenyl,
- haloalkyl,
- alkoxy,
- alkylthio, and
- —N(R₉)₂;

R₁is selected from the group consisting of:

- -R₄
- -X-R₄,
- -X-Y-R₄,
- -X-Y-X-Y-R₄, and
- -X-R₅;

R₃is selected from the group consisting of:

- -Z-R₄,
- -Z-X-R₄,
- -Z-X-Y-X-Y-R₄, and
- -Z-X-R₅;

Y is selected from the group consisting of:

Z is a bond or —O—;

R₅is selected from the group consisting of

R₆is selected from the group consisting of ═O and —S;

R₇is C_2-7alkylene;

R₈is selected from the group consisting of hydrogen, alkyl, alkoxyalkylenyl, hydroxyalkylenyl, arylalkylenyl, and heteroarylalkylenyl;

R₉is selected from the group consisting of hydrogen and alkyl;

R₁₀is C_3-8alkylene;

A is selected from the group consisting of —O—, —C(O)—, —CH₂—, —S(O)_0-2—, and —N(Q-R₄)—;

A′ is selected from the group consisting of —O—, —S(O)_0-2—, —N(-Q-R₄)—, and —CH₂—;

Q is selected from the group consisting of a bond, —C(R₆)—, —C(R₆)—C(R₆)—, —S(O)₂—, —C(R₆)—N(R₈)—W—, —S(O)₂—N(R₅)—, —C(R₆)—O—, —C(R₆)—S—, and —C(R₆)—N(OR₉)—;

V is selected from the group consisting of —C(R₆)—, —O—C(R₆)—, —N(R₈)—C(R₆)—, and —S(O)₂—;

W is selected from the group consisting of a bond, —C(O)—, and —S(O)₂—; and

a and b are independently integers from 1 to 6 with the proviso that a+b is ≦7;

or a pharmaceutically acceptable salt thereof to the animal.

As used herein “substantially less than the amount of TNF-α” means that there is at least a two-fold reduction in the maximal TNF-α response as determined using the test methods described herein.

As used herein, the terms “alkyl”, “alkenyl”, “alkynyl” and the prefix “alk-” are inclusive of both straight chain and branched chain groups and of cyclic groups, e.g., cycloalkyl and cycloalkenyl. Unless otherwise specified, these groups contain from 1 to 20 carbon atoms, with alkenyl groups containing from 2 to 20 carbon atoms, and alkynyl groups containing from 2 to 20 carbon atoms. In some embodiments, these groups have a total of up to 10 carbon atoms, up to 8 carbon atoms, up to 6 carbon atoms, or up to 4 carbon atoms. Cyclic groups can be monocyclic or polycyclic and preferably have from 3 to 10 ring carbon atoms. Exemplary cyclic groups include cyclopropyl, cyclopropylmethyl, cyclobutyl, cyclobutylmethyl, cyclopentyl, cyclopentylmethyl, cyclohexyl, cyclohexylmethyl, adamantyl, and substituted and unsubstituted bornyl, norbornyl, and norbornenyl.

Unless otherwise specified, “alkylene”, “alkenylene”, and “alkynylene” are the divalent forms of the “alkyl”, “alkenyl”, and “alkynyl” groups defined above. The terms, “alkylenyl”, “alkenylenyl”, and “alkynylenyl” are use when “alkylene”, “alkenylene”, and “alkynylene”, respectively, are substituted. For example, an arylalkylenyl group comprises an alkylene moiety to which an aryl group is attached.

The term “haloalkyl” is inclusive of groups that are substituted by one or more halogen atoms, including perfluorinated groups. This is also true of other groups that include the prefix “halo-.” Examples of suitable haloalkyl groups are chloromethyl, trifluoromethyl, 2,2,2-trifluoroethyl, and the like.

The term “aryl” as used herein includes carbocyclic aromatic rings or ring systems. Examples of aryl groups include phenyl, naphthyl, biphenyl, fluorenyl and indenyl.

Unless otherwise indicated, the term “heteroatom” refers to the atoms O, S, or N.

The term “heteroaryl” includes aromatic rings or ring systems that contain at least one ring heteroatom (e.g., O, S, N). In some embodiments, the term “heteroaryl” includes a ring or ring system that contains 2-12 carbon atoms, 1-3 rings, 1-4 heteroatoms, and O, S, and N as the heteroatoms. Exemplary heteroaryl groups include furyl, thienyl, pyridyl, quinolinyl, isoquinolinyl, indolyl, isoindolyl, triazolyl, pyrrolyl, tetrazolyl, imidazolyl, pyrazolyl, oxazolyl, thiazolyl, benzofuranyl, benzothiophenyl, carbazolyl, benzoxazolyl, pyrimidinyl, benzimidazolyl, quinoxalinyl, benzothiazolyl, naphthyridinyl, isoxazolyl, isothiazolyl, purinyl, quinazolinyl, pyrazinyl, 1-oxidopyridyl, pyridazinyl, triazinyl, tetrazinyl, oxadiazolyl, thiadiazolyl, and so on.

The term “heterocyclyl” includes non-aromatic rings or ring systems that contain at least one ring heteroatom (e.g., O, S, N) and includes all of the fully saturated and partially unsaturated derivatives of the above mentioned heteroaryl groups. In some embodiments, the term “heterocyclyl” includes a ring or ring system that contains 2-12 carbon atoms, 1-3 rings, 1-4 heteroatoms, and O, S, and N as the heteroatoms. Exemplary heterocyclyl groups include pyrrolidinyl, tetrahydrofuranyl, morpholinyl, thiomorpholinyl, 1,1-dioxothiomorpholinyl, piperidinyl, piperazinyl, thiazolidinyl, imidazolidinyl, isothiazolidinyl, tetrahydropyranyl, quinuclidinyl, homopiperidinyl (azepanyl), 1,4-oxazepanyl, homopiperazinyl (diazepanyl), 1,3-dioxolanyl, aziridinyl, azetidinyl, dihydroisoquinolin-(1H)-yl, octahydroisoquinolin-(1H)-yl, dihydroquinolin-(2H)-yl, octahydroquinolin-(2H)-yl, dihydro-1H-imidazolyl, 3-azabicyclo[3.2.2]non-3-yl, and the like.

The term “heterocyclyl” includes bicylic and tricyclic heterocyclic ring systems. Such ring systems include fused and/or bridged rings and spiro rings. Fused rings can include, in addition to a saturated or partially saturated ring, an aromatic ring, for example, a benzene ring. Spiro rings include two rings joined by one spiro atom and three rings joined by two spiro atoms. When “heterocyclyl” contains a nitrogen atom, the point of attachment of the heterocyclyl group may be the nitrogen atom.

The terms “arylene”, “heteroarylene”, and “heterocyclylene” are the divalent forms of the “aryl”, “heteroaryl”, and “heterocyclyl” groups defined above. The terms, “arylenyl”, “heteroarylenyl”, and “heterocyclylenyl” are used when “arylene”, “heteroarylene”, and “heterocyclylene”, respectively, are substituted. For example, an alkylarylenyl group comprises an arylene moiety to which an alkyl group is attached.

When a group (or substituent or variable) is present more than once in any Formula described herein, each group (or substituent or variable) is independently selected, whether explicitly stated or not. For example, for the formula —N(R₈)—C(O)—N(R₈)— each R₈group is independently selected. In another example, when R₁and R₃each contain an R₄group then each R₄group is independently selected. In a further example, when two Y groups are present and each Y group contains one or more R₈groups, then each Y group and each R₈group is independently selected.

The compounds described herein can be administered according to the methods of the present invention in any of the compounds' pharmaceutically acceptable forms, including isomers (e.g., diastereomers and enantiomers), salts, solvates, polymorphs, and the like. In particular, if a compound is optically active, the methods of the invention specifically include the use each of the compound's enantiomers as well as racemic mixtures of the enantiomers. It should be understood that the term “compound” includes any or all of such forms, whether explicitly stated or not (although at times, “salts” are explicitly stated).

The term “prodrug” means a compound that can be transformed in vivo to yield an immune response modifying compound, including any of the salt, solvated, polymorphic, or isomeric forms described above. The prodrug, itself, may be an immune response modifying compound, including any of the salt, solvated, polymorphic, or isomeric forms described above. The transformation may occur by various mechanisms, such as through a chemical (e.g., solvolysis or hydrolysis, for example, in the blood) or enzymatic biotransformation. A discussion of the use of prodrugs is provided by T. Higuchi and W. Stella, “Pro-drugs as Novel Delivery Systems,” Vol. 14 of the A. C. S. Symposium Series, and in Bioreversible Carriers in Drug Design, ed. Edward B. Roche, American Pharmaceutical Association and Pergamon Press, 1987.

For any of the compounds presented herein, each one of the following variables (e.g., X, Y, Z, R_A, R_B, R₁, R₃, R₄, R₅, Q, G₁, G₂, and so on) in any of its embodiments can be combined with any one or more of the other variables in any of their embodiments and associated with any one of the formulas described herein, as would be understood by one of skill in the art. Each of the resulting combinations of variables describes a compound or compounds which can be administered according to any one of the methods of the present invention, and the resulting method is an embodiment of the present invention.

For certain embodiments of any one of the above methods, n is 1.

For certain embodiments of any one of the above methods, n is 2.

For certain embodiments of any one of the above methods, including any one of the above embodiments, R_Aand R_Bform a fused benzene ring which is unsubstituted or substituted by one or more R groups, or substituted by one R₃group, or substituted by one R₃group and one R group. For certain of these embodiments, the fused benzene ring is substituted by an R₃group at the 7-position.

For certain embodiments of any one of the above methods, including any one of the above embodiments except where R_Aand R_Bform the fused benzene ring, R_Aand R_Bform a fused pyridine ring which is unsubstituted or substituted by one or more R groups, or substituted by one R₃group, or substituted by one R₃group and one R group.

For certain embodiments of any one of the above methods, including any one of the above embodiments except where R_Aand R_Bform the fused benzene or pyridine ring, R_Aand R_Bform a fused 5 to 7 membered saturated ring, optionally containing one heteroatom selected from the group consisting of N and S, wherein the ring is unsubstituted or substituted by one or more R groups.

For certain embodiments of any one of the above methods, including any one of the above embodiments except where R_Aand R_Bform the fused benzene, pyridine, or 5 to 7 membered saturated ring, R_Aand R_Bare each independently selected from the group consisting of hydrogen, halogen, alkyl, alkenyl, alkoxy, alkylthio, and —N(R₉)₂. For certain of these embodiments, R_Aand R_Bare each methyl.

For certain embodiments of any one of the above methods, including any one of the above embodiments, R₁is selected from the group consisting of -R₄, -X-R₄, -X-Y-R₄, -X-Y-X¹-Y¹-R₄, and -X-R₅; wherein X is alkylene that is optionally interrupted or terminated by heterocyclylene and optionally interrupted by one —O— group; Y is selected from the group consisting of —O—, —S(O)₂—, —S(O)₂—N(R₈)—, —C(O)—, —C(O)—O—, —O—C(O)—, —N(R₈)-Q-, —C(O)—N(R₈)—,

X¹is selected from the group consisting of alkylene and arylene; Y¹is selected from the group consisting of —S—, —C(O)—, —C(O)—O—, —C(O)—N(R₈)—, —S(O)₂—N(R₈)—, and —N(R₈)—C(O)—; R₄is selected from the group consisting of hydrogen, alkyl, aryl, heterocyclyl, heteroaryl, heteroarylalkylenyl, alkynyl, arylalkylenyl, and arylalkenylenyl, wherein the alkyl, aryl, arylalkylenyl, heterocyclyl, heteroaryl, and heteroarylalkylenyl groups can be unsubstituted or substituted by one or more substituents independently selected from the group consisting of alkyl, alkoxy, haloalkyl, haloalkoxy, halogen, hydroxy, cyano, aryl, aryloxy, heteroaryl, heterocyclyl, amino, dialkylamino, and in the case of alkyl and heterocyclyl, oxo; R₅is selected from the group consisting of:

R₆is selected from the group consisting of ═O and ═S; R₇is C_2-7alkylene; R₈is selected from the group consisting of hydrogen, alkyl, alkoxyalkylenyl, hydroxyalkylenyl, arylalkylenyl, and heteroarylalkylenyl; R₁₀is C_3-8alkylene; A is selected from the group consisting of —O—, —C(O)—, and —N(R₄)—; Q is selected from the group consisting of a bond, —C(R₆)—, —S(O)₂—, —C(R₆)—N(R₈)—W—, —S(O)₂—N(R₈)—, —C(O)—O—, and —C(O)—S—; W is selected from the group consisting of a bond and —C(O)—; and a and b are independently integers from 1 to 6 with the proviso that a+b is ≦7.

For certain embodiments of any one of the above methods, including any one of the above embodiments, R₁is selected from the group consisting of C_1-5alkyl, C_2-5alkynyl, arylC_1-4alkylenyl, cycloalkylC_1-4alkylenyl, C_1-4alkyl-S(O)₂—C_1-4alkylenyl, aryl-S(O)₂—C_1-4alkylenyl, C_1-4alkyl-S(O)₂—C_1-4alkylenyl-O—C_1-4alkylenyl, C_1-4alkyl-S(O)₂—NH—C_1-4alkylenyl, hydroxyC_1-4alkylenyl, dihydroxyC_1-4alkylenyl, haloC_1-4alkylenyl, aminoC_1-4alkylenyl, C_1-4alkyl-C(O)—O—C_1-4alkylenyl, C_1-6alkyl-C(O)—NH—C_1-4alkylenyl, aryl-C(O)—NH—C_1-4alkylenyl wherein aryl is unsubstituted or substituted with one or two halogen groups, heteroaryl-C(O)—NH—C_1-4alkylenyl, di(C_1-4alkyl)amino-S(O)₂—NH—C_1-4alkylenyl, aryl-S(O)₂—NH-C_1-4alkylenyl, aryl-NH—C(O)—NH—C_1-4alkylenyl, heteroaryl-NH—C(S)—NH—C_1-4alkylenyl, di(C_1-4alkyl)amino-C(O)—NH—C_1-4alkylenyl, C_1-4alkylamino-C(O)—NH—C_1-4alkylenyl, di(C_1-4alkyl)amino-S(O)₂—C_1-4alkylenyl, C_1-4alkylamino-S(O)₂—C_1-4alkylenyl, amino-S(O)₂—C_1-4alkylenyl, heteroarylC_1-4alkylenyl wherein heteroaryl is unsubstituted or substituted by a substituent selected from the group consisting of aryl, heteroaryl, and alkyl, and heterocyclylC_1-4alkylenyl wherein heterocyclyl is unsubstituted or substituted by one, two, or three substituents selected from the group consisting of alkyl, aryl, heteroaryl, and oxo.

For certain embodiments of any one of the above methods, including any one of the above embodiments, R₁is selected from the group consisting of methyl, ethyl, propyl, 2-methylpropyl, 2,2-dimethylpropyl, butyl, pent-4-ynyl, 2-phenylethyl, 2-hydroxy-2-methylpropyl, 2-fluoro-2-methylpropyl, 2,3-dihydroxypropyl, 4-hydroxybutyl, 2-amino-2-methylpropyl, 2-aminoethyl, 4-aminobutyl, 2-(methylsulfonyl)ethyl, 2-(propylsulfonyl)ethyl, 4-(methylsulfonyl)butyl, 2,2-dimethyl-3-(methylsulfonyl)propyl, 3-(phenylsulfonyl)propyl, 2-methyl-2-[2-(methylsulfonyl)ethoxy]propyl, 4-acetoxybutyl, 2-[(methylsulfonyl)amino]ethyl, 4-[(methylsulfonyl)amino]butyl, 2-methyl-2-[(methylsulfonyl)amino]propyl, 2-{[(1-methylethyl)sulfonyl]amino}ethyl, 2-(benzenesulfonylamino)ethyl, 2-(dimethylaminosulfonylamino)ethyl, 4-(aminosulfonyl)butyl, 4-[(methylamino)sulfonyl]butyl, 4-[(dimethylamino)sulfonyl]butyl, 2-[(cyclohexylcarbonyl)amino]-2-methylpropyl, 2-[(cyclopropylcarbonyl)amino]ethyl, 4-[(cyclopropylcarbonyl)amino]butyl, 2-[(cyclopropylcarbonyl)amino]-2-methylpropyl, 2-methyl-2-{[(1-methylethyl)carbonyl]amino}propyl, 2-methyl-2-[(ethylcarbonyl)amino]propyl, 2-methyl-2-[(pyridin-3-ylcarbonyl)amino]propyl, 2-methyl-2-[(pyridin-4-ylcarbonyl)amino]propyl, 2-(acetylamino)-2-methylpropyl, 2-(benzoylamino)ethyl, 2-(benzoylamino)-2-methylpropyl, 2-[(4-fluorobenzoyl)amino]-2-methylpropyl, 2-[(3,4-difluorobenzoyl)amino]-2-methylpropyl, 2-[(pyridin-3-ylcarbonyl)amino]ethyl, 2-{[(1-methylethyl)carbonyl]amino}ethyl, 4-{[(1-methylethyl)carbonyl]amino}butyl, 2-methyl-2-({[(1-methylethyl)amino]carbonyl}amino)propyl, 2-({[(1-methylethyl)amino]carbonyl}amino)ethyl, 4-(4-pyridin-2-ylpiperazin-1-yl)butyl, tetrahydro-2H-pyran-4-ylmethyl, 4-(1,1-dioxidoisothiazolidin-2-yl)butyl, (2,2-dimethyl-1,3-dioxolan-4-yl)methyl, 3-(3-methylisoxazol-5-yl)propyl, 3-(3-isopropylisoxazol-5-yl)propyl, 3-(3-phenylisoxazol-5-yl)propyl, 3-(3-pyridin-3-ylisoxazol-5-yl)propyl, 4-(3,5,5-trimethyl-1,2,4-oxadiazol-4(5H)-yl)butyl, 4-(3-methyl-1-oxa-2,4-diazaspiro[4.4]non-2-en-4-yl)butyl, 2-{[(pyridin-3-ylamino)carbonothioyl]amino}ethyl, 2-{[(dimethylamino)carbonyl]amino}ethyl, and 2-{[(phenylamino)carbonyl]amino}ethyl.

For certain embodiments of any one of the above methods, including any one of the above embodiments, R₁is selected from the group consisting of alkyl, aminoalkyl, dihydroxyalkyl, haloalkyl, and hydroxyalkyl, except where R₁as defined does not include this definition. For certain of these embodiments, R₁is selected from the group consisting of methyl, ethyl, n-propyl, n-butyl, 2-methylpropyl, 2-amino-2-methylpropyl, 3-amino-2,2-dimethylpropyl, 2,3-dihydroxypropyl, 2-fluoro-2-methylpropyl, and 2-hydroxy-2-methylpropyl. Alternatively, for certain of these embodiments, R₁is selected from the group consisting of (1-hydroxycyclobutyl)methyl, (1-hydroxycyclopentyl)methyl, and (1-hydroxycyclohexyl)methyl.

For certain embodiments of any one of the above methods, including any one of the above embodiments, R₁is heterocyclylalkylenyl wherein heterocyclyl is unsubstituted or substituted by one or more substituents independently selected from the group consisting of alkyl, aryl, heteroaryl, hydroxy, and oxo, except where R₁as defined does not include this definition. For certain of these embodiments as well as any one of the above embodiments wherein R₁as defined includes heterocyclyl, heterocyclyl is selected from the group consisting of 1,3-dioxolanyl, tetrahydropyranyl, tetrahydrofuranyl, pyrrolidinyl, piperidinyl, and morpholinyl, each of which is unsubstituted or substituted by one, two, or three substituents selected from the group consisting of alkyl, aryl, heteroaryl, and oxo. For certain of these embodiments wherein R₁is heterocyclylalkylenyl, heterocyclyl is selected from the group consisting of 1,3-dioxolanyl, tetrahydropyranyl, tetrahydrofuranyl, pyrrolidinyl, piperidinyl, and morpholinyl, and alkylenyl is C_1-4alkylenyl. For certain of these embodiments, R₁is selected from the group consisting of tetrahydro-2H-pyran-4-ylmethyl and (2,2-dimethyl-1,3-dioxolan-4-yl)methyl. Alternatively, for certain of these embodiments, R₁is (4-hydroxytetrahydro-2H-pyran-4-yl)methyl.

For certain embodiments of any one of the above methods, including any one of the above embodiments, R₁is -X-Y-R₄, except where R₁as defined does not include this definition, wherein X is C_1-6alkylene which may be interrupted by one —O— group; Y is selected from the group consisting of —N(R₈)—C(O)—, —N(R₈)—S(O)₂—, —N(R₈)—C(O)—N(R₈)—, and —S(O)₂— wherein R₈is selected from hydrogen and methyl; and R₄is selected from the group consisting of C_1-6alkyl, isoquinolinyl, N-methylimidazolyl, pyridinyl, quinolinyl, phenyl, and phenyl substituted by a substituent selected from the group consisting of chloro, cyano, fluoro, hydroxy, and methyl. For certain of these embodiments, R₁is selected from the group consisting of 2-[(cyclopropylcarbonyl)amino]ethyl, 4-[(cyclopropylcarbonyl)amino]butyl, 2-[(cyclohexylcarbonyl)amino]-2-methylpropyl, 2-{[(1-methylethyl)carbonyl]amino}ethyl, 4-{[(1-methylethyl)carbonyl]amino}butyl, 2-methyl-2-{[(1-methylethyl)carbonyl]amino}propyl, 2-[(methylsulfonyl)amino]ethyl, 4-[(methylsulfonyl)amino]butyl, 2-methyl-2-[(methylsulfonyl)amino]propyl, 2-methyl-2-({[(1-methylethyl)amino]carbonyl}amino)propyl, 2-methyl-2-[2-(methylsulfonyl)ethoxy]propyl, and 2,2-dimethyl-3-(methylsulfonyl)propy.

wherein Q is —C(O)—, —C(O)—NH—, or S(O)₂—, R₁₀is pentylene, R₈is hydrogen or methyl; and R₄is selected from the group consisting of C_1-6alkyl, hydroxyC_1-6alkyl, isoquinolinyl, N-methylimidazolyl, pyridinyl, quinolinyl, benzyl, 1-phenylethyl, phenyl, and phenyl substituted by a substituent selected from the group consisting of chloro, cyano, fluoro, hydroxy, and methyl. For certain of these embodiments, X is C_1-6alkylene, Y is selected from the group consisting of —N(R₈)—C(O)—, —N(R₈)—S(O)₂—, and —N(R₈)—C(O)—N(R₈)—, and R₄is selected from the group consisting of C_1-4alkyl, hydroxyC_1-4alkyl, pyridinyl, benzyl, 1-phenylethyl, phenyl, and phenyl substituted by a substituent selected from the group consisting of chloro, cyano, fluoro, hydroxy, and methyl. Alternatively, for certain of these embodiments, X is C_1-6alkylene, Y is

wherein Q is —C(O)—, —C(O)—NH—, or S(O)₂—, and R₁₀is pentylene, and R₄is C_1-4alkyl. For certain of these embodiments where Y is

X is methylene. Alternatively, for certain of these embodiments, Y is —NH—S(O)₂—N(R₈)—, R₈is methyl, and R₄is C_1-4alkyl. For certain of these embodiments where Y is —NH—S(O)₂—N(R₈)—, X is C_2-6alkylene.

For certain embodiments of any one of the above methods, including any one of the above embodiments, R₁is -X-R₅, except where R₁as defined does not include this definition, wherein X is C_1-6alkylene, and R₅is

For certain of these embodiments, R₅is

wherein R₈is hydrogen, A is —O—, —CH₂—, or —N(Q-R₄)—, and a and b are each 2. For certain of these embodiments, Q-R₄is methyl. Alternatively, for certain of these embodiments, R₅is

For certain of these embodiments, R₁is selected from the group consisting of 4-(1,1-dioxidoisothiazolidin-2-yl)butyl, 4-[(4-morpholinecarbonyl)amino]butyl, and 2-[(4-morpholinecarbonyl)amino]ethyl.

wherein R₆is ═O, R₇is propylene, R₁₀is pentylene, A is —O—, and a and b are each 2. For certain of these embodiments, X is ethylene or butylene.

For certain embodiments of any one of the above methods, including any one of the above embodiments having an R₃group, R₃is selected from the group consisting of aryl, arylalkyleneoxy, heteroaryl, and heteroaryalkyleneoxy, wherein aryl, arylalkyleneoxy, heteroaryl, and heteroarylalkylenoxy are unsubstituted or substituted with one or more substituents selected from the group consisting of alkyl, alkoxy, halogen, hydroxy, and hydroxyalkyl. For certain of these embodiments, R₃is phenyl, pyridin-2-yl, pyridin-3-yl, pyridin-4-yl, quinolin-3-yl, or thiazol-4-ylmethoxy, any of which may be unsubstituted or substituted by one or more substituents selected from the group consisting of alkyl, alkoxy, halogen, hydroxy, and hydroxyalkyl. For certain of these embodiments, R₃is selected from the group consisting of pyridin-3-yl, pyridin-4-yl, 6-fluoropyridin-3-yl, 5-(hydroxymethyl)pyridin-3-yl, 2-ethoxyphenyl, quinolin-3-yl, and thiazol-4-ylmethoxy.

For certain embodiments of any one of the above methods, including any one of the above embodiments having an R₃group, R₃is thien-3-yl, phenyl, pyridin-2-yl, pyridin-3-yl, pyridin-4-yl, or quinolin-3-yl any of which may be unsubstituted or substituted by one or more substituents selected from the group consisting of alkyl, alkoxy, halogen, cyano, hydroxy, and hydroxyalkyl, except where R₃as defined does not include this definition.

For certain embodiments of any one of the above methods, including any one of the above embodiments having an R₃group, R₃is -Z-X-Y-R₄, except where R₃as defined does not include this definition, wherein Z is a bond, X is phenylene, Y is selected from the group consisting of —C(O)—, —C(O)—N(R₈)—, —N(R₉)—C(O)—, —N(R₈)—S(O)₂—, and —N(R₈)—C(O)—N(R₈)— wherein R₈is selected from hydrogen and methyl; and R₄is selected from the group consisting of C_1-6alkyl, morpholin-4-yl, phenyl, and phenyl substituted by a substituent selected from the group consisting of allyl, alkoxy, halogen, hydroxy, and hydroxyalkyl. For certain of these embodiments, R₃is 2-(4-morpholinecarbonyl)phenyl.

For certain embodiments of any one of the above methods, including any one of the above embodiments having an R₃group, except where R₃as defined does not include this definition, R₃is -X-Y-R₄, wherein X is phenylene, Y is selected from the group consisting of —C(O)—, —C(O)—N(R₈)—, —N(R₈)—C(O)—, —N(R₈)—S(O)₂—, and —N(R₈)—C(O)—N(R₈)— wherein R₈is selected from hydrogen and methyl; and R₄is selected from the group consisting of C_1-6alkyl, phenyl, and phenyl substituted by a substituent selected from the group consisting of alkyl, alkoxy, halogen, hydroxy, and hydroxyalkyl; with the proviso that when Y is —C(O)—N(R₈)— or —N(R₈)—C(O)—N(R₈)— then R₄can also be hydrogen; and with the further proviso that when Y is —C(O)— or —N(R₈)—C(O)— then R₄can also be morpholin-4-yl, piperidin-1-yl, or pyrrolidin-1-yl. For certain of these embodiments, Y is —C(O)—NH—, and R₄is hydrogen or C_1-4alkyl. For certain of these embodiments, R₄is hydrogen. Alternatively, for certain of these embodiments, Y is —NH—C(O)—, and R₄is C_1-4alkyl. Alternatively, for certain of these embodiments, Y is —C(O)—, and R₄is morpholin-4-yl, piperidin-1-yl, or pyrrolidin-1-yl. For certain of these embodiments, R₃is 3-(methylsulfonylamino)phenyl, 3-(pyrrolidin-1-ylcarbonyl)phenyl, or 3-(morpholin-4-ylcarbonyl)phenyl.

For certain embodiments of any one of the above methods, including any one of the above embodiments which includes an R group, R is not present.

For certain embodiments of any one of the above methods, including any one of the above embodiments which includes an R group and an R₃group, neither R₃nor R is present.

For certain embodiments of any one of the above methods, including any one of the above embodiments which includes an R group, R is selected from the group consisting of hydroxy and methoxy.

For certain embodiments of any one of the above methods, including any one of the above embodiments which includes an R₃group, R₃is not present.

For certain embodiments of any one of the above methods, including any one of the above embodiments, an effective amount of the compound or salt is administered as a pharmaceutical composition comprising a therapeutically effective amount of the compound or salt and a pharmaceutically acceptable carrier.

For certain embodiments, the present invention provides a method of treating a viral disease in an animal in need thereof comprising preferentially inducing the biosynthesis of IFN-α in the animal according to any one of the above methods.

For certain embodiments, the present invention provides a method of treating a neoplastic disease in an animal in need thereof comprising preferentially inducing the biosynthesis of IFN-α in the animal according to any one of the above methods.

For certain embodiments of any one of the above methods, including any one of the above embodiments, the compound or salt is administered systemically.

For certain embodiments, R_Aand R_Bform a fused aryl ring or heteroaryl ring containing one heteroatom selected from the group consisting of N and S wherein the aryl or heteroaryl ring is unsubstituted or substituted by one or more R groups, or substituted by one R₃group, or substituted by one R₃group and one R group.

For certain embodiments, R_Aand R_Bform a fused aryl ring which is unsubstituted or substituted by one or more R groups, or substituted by one R₃group, or substituted by one R₃group and one R group.

For certain embodiments R_Aand R_Bform a fused benzene ring which is unsubstituted or substituted by one or more R groups, or substituted by one R₃group, or substituted by one R₃group and one R group.

For certain embodiments R_Aand R_Bform a fused benzene ring which is unsubstituted.

For certain embodiments R_Aand R_Bform a fused benzene ring which is substituted by one or more R groups, or substituted by one R₃group, or substituted by one R₃group and one R group.

For certain embodiments R_Aand R_Bform a fused benzene ring which is substituted by one R₃group. For certain of these embodiments, the R₃group is at the 7-position.

For certain embodiments R_Aand R_Bform a fused benzene ring which is substituted by one or more R groups.

For certain embodiments, R_Aand R_Bform a fused heteroaryl ring containing one heteroatom selected from the group consisting of N and S wherein the heteroaryl ring is unsubstituted or substituted by one or more R groups, or substituted by one R₃group, or substituted by one R₃group and one R group.

For certain embodiments, R_Aand R_Bform a fused pyridine ring which is unsubstituted or substituted by one or more R groups, or substituted by one R₃group, or substituted by one R₃group and one R group.

For certain embodiments, R_Aand R_Bform a fused pyridine ring which is unsubstituted.

For certain embodiments, R_Aand R_Bform a fused pyridine ring which is substituted by one or more R groups, or substituted by one R₃group, or substituted by one

R₃group and one R group. For certain of these embodiments, the fused pyridine ring is

wherein the highlighted bond is the position where the ring is fused. For certain or these embodiments, R_Aand R_Bform a fused pyridine ring which is substituted by one R₃group. For certain of these embodiments, the fused pyridine ring is

wherein the highlighted bond is the position where the ring is fused. For certain of these embodiments, the R₃group is at the 7-position.

For certain embodiments, R_Aand R_Bform a fused pyridine ring which is substituted by one or more R groups.

For certain embodiments, R_Aand R_Bform a fused 5 to 7 membered saturated ring, optionally containing one heteroatom selected from the group consisting of N and S, and unsubstituted or substituted at a carbon atom by one or more R groups.

For certain embodiments, R_Aand R_Bform a fused 5 to 7 membered saturated ring, wherein the ring is unsubstituted or substituted by one or more R groups. For certain of these embodiments, the fused ring is a cyclohexene ring wherein the double bond is the position where the ring is fused. For certain of these embodiments, the fused cyclohexene ring is unsubstituted.

For certain embodiments, R_Aand R_Bform a fused 5 to 7 membered saturated ring, containing one nitrogen atom, and unsubstituted or substituted at a carbon atom by one or more R groups. For certain of these embodiments, the fused ring is a tetrahydropyridine ring. For certain of these embodiments, the fused tetrahydropyridine ring is

wherein the highlighted bond indicates the position where the ring is fused. For certain of these embodiments, the fused tetrahydropyridine ring is unsubstituted.

For certain embodiments, R_Aand R_Bare each independently selected from the group consisting of hydrogen, halogen, alkyl, alkenyl, alkoxy, alkylthio, and —N(R₉)₂. For certain of these embodiments, R_Aand R_Bare each independently alkyl. For certain of these embodiments, R_Aand R_Bare each methyl.

For certain embodiments, R is selected from the group consisting of halogen, hydroxy, alkyl, alkenyl, haloalkyl, alkoxy, alkylthio, and —N(R₉)₂.

For certain embodiments, R is selected from the group consisting of hydroxy and methoxy.

For certain embodiments, R is not present.

For certain embodiments, R₁is selected from the group consisting of -R₄, -X-R₄, -X-Y-R₄, -X-Y-X-Y-R₄, and -X-R₅.

For certain embodiments, R₁is selected from the group consisting of -R₄, -X-R₄, -X-Y-R₄, -X-Y-X¹-Y¹-R₄, and -X-R₅; wherein X is alkylene that is optionally interrupted or terminated by heterocyclylene and optionally interrupted by one —O— group; Y is selected from the group consisting of —O—, —S(O)₂—, —S(O)₂—N(R₈)—, —C(O)—, —C(O)—O—, —O—C(O)—, —N(R₈)-Q-, —C(O)—N(R₈)—,

For certain embodiments, R₁is selected from the group consisting of C_1-5alkyl, C_2-5alkynyl, arylC_1-4alkylenyl, cycloalkylC_1-4alkylenyl, C_1-4alkyl-S(O)₂—C_1-4alkylenyl, aryl-S(O)₂—C_1-4alkylenyl, C_1-4alkyl-S(O)₂—C_1-4alkylenyl-O—C_1-4alkylenyl, C_1-4alkyl-S(O)₂—NH—C_1-4alkylenyl, hydroxyC_1-4alkylenyl, dihydroxyC_1-4alkylenyl, haloC_1-4alkylenyl, aminoC_1-4alkylenyl, C_1-4alkyl-C(O)—O—C_1-4alkylenyl, C_1-6alkyl-C(O)—NH—C_1-4alkylenyl, aryl-C(O)—NH—C_1-4alkylenyl wherein aryl is unsubstituted or substituted with one or two halogen groups, heteroaryl-C(O)—NH—C_1-4alkylenyl, di(C_1-4alkyl)amino-S(O)₂—NH—C_1-4alkylenyl, aryl-S(O)₂—NH—C_1-4alkylenyl, aryl-NH—C(O)—NH—C_1-4alkylenyl, heteroaryl-NH—C(S)—NH—C_1-4alkylenyl, di(C_1-4alkyl)amino-C(O)—NH—C_1-4alkylenyl, C_1-4alkylamino-C(O)—NH—C_1-4alkylenyl, di(C_1-4alkyl)amino-S(O)₂—C_1-4alkylenyl, C_1-4alkylamino-S(O)₂—C_1-4alkylenyl, amino-S(O)₂—C_1-4alkylenyl, heteroarylC_1-4alkylenyl wherein heteroaryl is unsubstituted or substituted by a substituent selected from the group consisting of aryl, heteroaryl, and alkyl, and heterocyclylC_1-4alkylenyl wherein heterocyclyl is unsubstituted or substituted by one, two, or three substituents selected from the group consisting of alkyl, aryl, heteroaryl, and oxo.

For certain embodiments, R₁is selected from the group consisting of methyl, ethyl, propyl, 2-methylpropyl, 2,2-dimethylpropyl, butyl, pent-4-ynyl, 2-phenylethyl, 2-hydroxy-2-methylpropyl, 2-fluoro-2-methylpropyl, 2,3-dihydroxypropyl, 4-hydroxybutyl, 2-amino-2-methylpropyl, 2-aminoethyl, 4-aminobutyl, 2-(methylsulfonyl)ethyl, 2-(propylsulfonyl)ethyl, 4-(methylsulfonyl)butyl, 2,2-dimethyl-3-(methylsulfonyl)propyl, 3-(phenylsulfonyl)propyl, 2-methyl-2-[2-(methylsulfonyl)ethoxy]propyl, 4-acetoxybutyl, 2[(methylsulfonyl)amino]ethyl, 4-[(methylsulfonyl)amino]butyl, 2-methyl-2-[(methylsulfonyl)amino]propyl, 2-{[(1-methylethyl)sulfonyl]amino}ethyl, 2-(benzenesulfonylamino)ethyl, 2-(dimethylaminosulfonylamino)ethyl, 4-(aminosulfonyl)butyl, 4-[(methylamino)sulfonyl]butyl, 4-[(dimethylamino)sulfonyl]butyl, 2-[(cyclohexylcarbonyl)amino]-2-methylpropyl, 2-[(cyclopropylcarbonyl)amino]ethyl, 4-[(cyclopropylcarbonyl)amino]butyl, 2-[(cyclopropylcarbonyl)amino]-2-methylpropyl, 2-methyl-2-{[(1-methylethyl)carbonyl]amino}propyl, 2-methyl-2-[(ethylcarbonyl)amino]propyl, 2-methyl-2-[(pyridin-3-ylcarbonyl)amino]propyl, 2-methyl-2-[(pyridin-4-ylcarbonyl)amino]propyl, 2-(acetylamino)-2-methylpropyl, 2-(benzoylamino)ethyl, 2-(benzoylamino)-2-methylpropyl, 2-[(4-fluorobenzoyl)amino]-2-methylpropyl, 2-[(3,4-difluorobenzoyl)amino]-2-methylpropyl, 2-[(pyridin-3-ylcarbonyl)amino]ethyl, 2-{[(1-methylethyl)carbonyl]amino}ethyl, 4-{[(1-methylethyl)carbonyl]amino}butyl, 2-methyl-2-({[(1-methylethyl)amino]carbonyl}amino)propyl, 2-({[(1-methylethyl)amino]carbonyl}amino)ethyl, 4-(4-pyridin-2-ylpiperazin-1-yl)butyl, tetrahydro-2H-pyran-4-ylmethyl, 4-(1,1-dioxidoisothiazolidin-2-yl)butyl, (2,2-dimethyl-1,3-dioxolan-4-yl)methyl, 3-(3-methylisoxazol-5-yl)propyl, 3-(3-isopropylisoxazol-5-yl)propyl, 3-(3-phenylisoxazol-5-yl)propyl, 3-(3-pyridin-3-ylisoxazol-5-yl)propyl, 4-(3,5,5-trimethyl-1,2,4-oxadiazol-4(5H)-yl)butyl, 4-(3-methyl-1-oxa-2,4-diazaspiro[4.4]non-2-en-4-yl)butyl, 2-{[(pyridin-3-ylamino)carbonothioyl]amino}ethyl, 2-{[(dimethylamino)carbonyl]amino}ethyl, and 2-{[(phenylamino)carbonyl]amino}ethyl.

For certain embodiments, R₁is -R₄.

For certain embodiments, R₁is selected from the group consisting of alkyl, aminoalkyl, dihydroxyalkyl, haloalkyl, and hydroxyalkyl.

For certain embodiments, R₁is selected from the group consisting of methyl, ethyl, n-propyl, n-butyl, 2-methylpropyl, 2-amino-2-methylpropyl, 3-amino-2,2-dimethylpropyl, 2,3-dihydroxypropyl, 2-fluoro-2-methylpropyl, and 2-hydroxy-2-methylpropyl.

For certain embodiments, R₁is selected from the group consisting of (1-hydroxycyclobutyl)methyl, (1-hydroxycyclopentyl)methyl, and (1-hydroxycyclohexyl)methyl.

For certain embodiments, R₁is heterocyclylalkylenyl wherein heterocyclyl is unsubstituted or substituted by one or more substituents independently selected from the group consisting of alkyl, aryl, heteroaryl, hydroxy, and oxo.

For certain embodiments, R₁is heterocyclylalkylenyl wherein heterocyclyl is selected from the group consisting of 1,3-dioxolanyl, tetrahydropyranyl, tetrahydrofuranyl, pyrrolidinyl, piperidinyl, and morpholinyl, and alkylenyl is C_1-4alkylenyl.

For certain embodiments, R₁is selected from the group consisting of tetrahydro-2H-pyran-4-ylmethyl and (2,2-dimethyl-1,3-dioxolan-4-yl)methyl.

For certain embodiments, R₁is (4-hydroxytetrahydro-2H-pyran-4-yl)methyl.

For certain embodiments, R₁is -X-Y-R₄.

For certain embodiments, R₁is -X-Y-R₄wherein X is C_1-6alkylene which may be interrupted by one —O— group; Y is selected from the group consisting of —N(R₉)—C(O)—, —N(R₉)—S(O)₂—, —N(R₈)—C(O)—N(R₈)—, and —S(O)₂— wherein R₈is selected from hydrogen and methyl; and R₄is selected from the group consisting of C_1-6alkyl, isoquinolinyl, N-methylimidazolyl, pyridinyl, quinolinyl, phenyl, and phenyl substituted by a substituent selected from the group consisting of chloro, cyano, fluoro, hydroxy, and methyl.

For certain embodiments, R₁is selected from the group consisting of 2-[(cyclopropylcarbonyl)amino]ethyl, 4-[(cyclopropylcarbonyl)amino]butyl, 2-[(cyclohexylcarbonyl)amino]-2-methylpropyl, 2-{[(1-methylethyl)carbonyl]amino}ethyl, 4-{[(1-methylethyl)carbonyl]amino}butyl, 2-methyl-2-{[(1-methylethyl)carbonyl]amino}propyl, 2-[(methylsulfonyl)amino]ethyl, 4-[(methylsulfonyl)amino]butyl, 2-methyl-2-[(methylsulfonyl)amino]propyl, 2-methyl-2-({[(1-methylethyl)amino]carbonyl}amino)propyl, 2-methyl-2-[2-(methylsulfonyl)ethoxy]propyl, and 2,2-dimethyl-3-(methylsulfonyl)propyl.

For certain embodiments, R₁is -X-Y-R₄wherein X is C_1-6alkylene which may be interrupted by an —O— group; Y is selected from the group consisting of —N(R₈)—C(O)—, —N(R₈)—S(O)₂—, —N(R₈)—C(O)—N(R₈)—, —N(R₈)—S(O)₂—N(R₈)—, —S(O)₂—, and

For certain embodiments, R₁is -X-Y-R₄wherein X is C_1-4alkylene; Y is

and R₄is C_1-4alkyl. For certain of these embodiments, R₁₀is pentylene, and Q is selected from the group consisting of —S(O)₂—, —C(O)—, and —C(O)—NH—.

For certain embodiments, R₁is -X-Y-R₄wherein Y is —NH—S(O)₂—N(R₈)—, R₈is methyl, and R₄is C_1-4alkyl.

For certain embodiments, R₁is -X-R₅.

For certain embodiments, R₁is -X-R₅wherein X is C_1-6alkylene, and R₅is

wherein R₈is hydrogen, A is —O—, —CH₂—, or —N(Q-R₄)—, and a and b are each 2.

For certain embodiments, R₁is -X-R₅wherein X is C_1-6alkylene, R₅is

and R₇is propylene.

For certain embodiments, R₁is -X-R₅, wherein X is C_1-4alkylene, and R₅is

wherein R₆is ═O, R₇is propylene, R₁₀is pentylene, A is —O—, and a and b are each 2.

For certain embodiments, R₁is selected from the group consisting of 4-(1,1-dioxidoisothiazolidin-2-yl)butyl, 4-[(4-morpholinecarbonyl)amino]butyl, and 2-[(4-morpholinecarbonyl)amino]ethyl.

For certain embodiments, R₃is selected from the group consisting of -Z-R₄, -Z-X-R₄, -Z-X-Y-R₄, -Z-X-Y-X-Y-R₄, and -Z-X-R₅.

For certain embodiments, R₃is -Z-R₄.

For certain embodiments, R₃is selected from the group consisting of aryl, arylalkyleneoxy, heteroaryl, and heteroarylalkyleneoxy, wherein aryl, arylalkyleneoxy, heteroaryl, and heteroarylalkyleneoxy are unsubstituted or substituted with one or more substituents selected from the group consisting of alkyl, alkoxy, halogen, hydroxy, and hydroxyalkyl.

For certain embodiments, R₃is selected from the group consisting of aryl, arylalkyleneoxy, and heteroaryl, wherein aryl, arylalkyleneoxy, and heteroaryl are unsubstituted or substituted with one or more substituents selected from the group consisting of alkyl, alkoxy, halogen, hydroxy, and hydroxyalkyl.

For certain embodiments, R₃is thien-3-yl, phenyl, pyridin-2-yl, pyridin-3-yl, pyridin-4-yl, or quinolin-3-yl any of which may be unsubstituted or substituted by one or more substituents selected from the group consisting of alkyl, alkoxy, halogen, cyano, hydroxy, and hydroxyalkyl.

For certain embodiments, R₃is phenyl, pyridin-2-yl, pyridin-3-yl, pyridin-4-yl, quinolin-3-yl, or thiazol-4-ylmethoxy, any of which may be unsubstituted or substituted by one or more substituents selected from the group consisting of alkyl, alkoxy, halogen, hydroxy, and hydroxyalkyl.

For certain embodiments, R₃is selected from the group consisting of pyridin-3-yl, pyridin-4-yl, 6-fluoropyridin-3-yl, 5-(hydroxymethyl)pyridin-3-yl, 2-ethoxyphenyl, quinolin-3-yl, or thiazol-4-ylmethoxy.

For certain embodiments, R₃is -Z-X-Y-R₄.

For certain embodiments, R₃is -Z-X-Y-R₄wherein X is phenylene, Y is selected from the group consisting of —C(O)—, —C(O)—N(R₈)—, —N(R₈)—C(O)—, —N(R₈)—S(O)₂—, and —N(R₈)—C(O)—N(R₈)— wherein R₈is selected from hydrogen and methyl; Z is a bond; and R₄is selected from the group consisting of C_1-6alkyl, phenyl, morpholin-4-yl, and phenyl substituted by a substituent selected from the group consisting of alkyl, alkoxy, halogen, hydroxy, and hydroxyalkyl.

For certain embodiments, R₃is -Z-X-Y-R₄wherein X is phenylene, Y is selected from the group consisting of —N(R₈)—C(O)—, —N(R₈)—S(O)₂—, and —N(R₈)—C(O)—N(R₈)— wherein R₈is selected from hydrogen and methyl; Z is a bond; and R₄is selected from the group consisting of C_1-6alkyl, phenyl, and phenyl substituted by a substituent selected from the group consisting of alkyl, alkoxy, halogen, hydroxy, and hydroxyalkyl.

For certain embodiments, R₃is -Z-X-Y-R₄, wherein Z is a bond, X is phenylene, Y is selected from the group consisting of —C(O)—, —C(O)—N(R₈)—, —N(R₈)—C(O)—, —N(R₈)—S(O)₂—, and —N(R₈)—C(O)—N(R₈)— wherein R₈is selected from hydrogen and methyl; and R₄is selected from the group consisting of C_1-6alkyl, phenyl, and phenyl substituted by a substituent selected from the group consisting of alkyl, alkoxy, halogen, hydroxy, and hydroxyalkyl; with the proviso that when Y is —C(O)—N(R₈)— or —N(R₈)—C(O)—N(R₈)— then R₄can also be hydrogen; and with the further proviso that when Y is —C(O)— or —N(R₈)—C(O)— then R₄can also be morpholin-4-yl, piperidin-1-yl, or pyrrolidin-1-yl.

For certain embodiments, R₃is -Z-X-Y-R₄, wherein Z is a bond, X is phenylene, Y is —C(O)—NH—, and R₄is hydrogen or C_1-4alkyl.

For certain embodiments, R₃is -Z-X-Y-R₄, wherein Z is a bond, X is phenylene, Y is —NH—C(O)—, and R₄is C_1-4alkyl.

For certain embodiments, R₃is -Z-X-Y-R₄, wherein Z is a bond, X is phenylene, Y is —C(O)—, and R₄is morpholin-4-yl, piperidin-1-yl, or pyrrolidin-1-yl.

For certain embodiments, R₃is 3-(methylsulfonylamino)phenyl, 3-(pyrrolidin-1-ylcarbonyl)phenyl, or 3-(morpholin-4-ylcarbonyl)phenyl.

For certain embodiments, R₃is 2-(4-morpholinecarbonyl)phenyl.

For certain embodiments, R₃is not present.

For certain embodiments, neither R₃nor R is present.

For certain embodiments, R₄is selected from the group consisting of hydrogen, alkyl, alkenyl, alkynyl, aryl, arylalkylenyl, aryloxyalkylenyl, alkylarylenyl, heteroaryl, heteroarylalkylenyl, heteroaryloxyalkylenyl, alkylheteroarylenyl, and heterocyclyl wherein the alkyl, alkenyl, alkynyl, aryl, arylalkylenyl, aryloxyalkylenyl, alkylarylenyl, heteroaryl, heteroarylalkylenyl, heteroaryloxyalkylenyl, alkylheteroarylenyl, and heterocyclyl groups can be unsubstituted or substituted by one or more substituents independently selected from the group consisting of alkyl, alkoxy, hydroxyalkyl, haloalkyl, haloalkoxy, halogen, nitro, hydroxy, mercapto, cyano, aryl, aryloxy, arylalkyleneoxy, heteroaryl, heteroaryloxy, heteroarylalkyleneoxy, heterocyclyl, amino, alkylamino, dialkylamino, (dialkylamino)alkyleneoxy, and in the case of alkyl, alkenyl, alkynyl, and heterocyclyl, oxo.

For certain embodiments, R₄is selected from the group consisting of hydrogen, alkyl, alkenyl, aryl, arylalkylenyl, heteroaryl, heteroarylalkylenyl, and heterocyclyl, wherein the alkyl, alkenyl, aryl, arylalkylenyl, heteroaryl, heteroarylalkylenyl, and heterocyclyl groups can be unsubstituted or substituted by one or more substituents independently selected from the group consisting of alkyl, alkoxy, hydroxyalkyl, haloalkyl, haloalkoxy, halogen, nitro, hydroxy, mercapto, cyano, aryl, aryloxy, heteroaryl, heteroaryloxy, heterocyclyl, amino, alkylamino, dialkylamino, and, in the case of alkyl, alkenyl, and heterocyclyl, oxo.

For certain embodiments, R₄is selected from the group consisting of hydrogen, alkyl, alkenyl, aryl, arylalkylenyl, heteroaryl, and heteroarylalkylenyl, wherein the alkyl, alkenyl, aryl, arylalkylenyl, heteroaryl, and heteroarylalkylenyl, groups can be unsubstituted or substituted by one or more substituents independently selected from the group consisting of alkyl, alkoxy, hydroxyalkyl, haloalkyl, haloalkoxy, halogen, nitro, hydroxy, mercapto, cyano, aryl, aryloxy, heteroaryl, heteroaryloxy, heterocyclyl, amino, alkylamino, dialkylamino, and, in the case of alkyl and alkenyl, oxo.

For certain embodiments, R₄is selected from the group consisting of hydrogen, alkyl, aryl, heterocyclyl, heteroaryl, heteroarylalkylenyl, alkynyl, arylalkylenyl, and arylalkenylenyl, wherein the alkyl, aryl, arylalkylenyl, heterocyclyl, heteroaryl, and heteroarylalkylenyl groups can be unsubstituted or substituted by one or more substituents independently selected from the group consisting of alkyl, alkoxy, haloalkyl, haloalkoxy, halogen, hydroxy, cyano, aryl, aryloxy, heteroaryl, heterocyclyl, amino, dialkylamino, and in the case of alkyl and heterocyclyl, oxo.

For certain embodiments, R₄is selected from the group consisting of C_1-6alkyl, hydroxyC_1-6alkyl, isoquinolinyl, N-methylimidazolyl, pyridinyl, quinolinyl, benzyl, 1-phenylethyl, phenyl, and phenyl substituted by a substituent selected from the group consisting of chloro, cyano, fluoro, hydroxy, and methyl.

For certain embodiments, R₄is selected from the group consisting of C_1-6alkyl, isoquinolinyl, N-methylimidazolyl, pyridinyl, quinolinyl, phenyl, and phenyl substituted by a substituent selected from the group consisting of chloro, cyano, fluoro, hydroxy, and methyl.

For certain embodiments, R₄is selected from the group consisting of C_1-6alkyl, morpholin-4-yl, phenyl, and phenyl substituted by a substituent selected from the group consisting of alkyl, alkoxy, halogen, hydroxy, and hydroxyalkyl.

For certain embodiments, R₄is selected from the group consisting of C_1-6alkyl, phenyl, and phenyl substituted by a substituent selected from the group consisting of alkyl, alkoxy, halogen, hydroxy, and hydroxyalkyl.

For certain embodiments, R₄is morpholin-4-yl, piperidin-1-yl, or pyrrolidin-1-yl.

For certain embodiments, R₄is C_1-6alkyl.

For certain embodiments, R₄is hydrogen or C_1-4allyl.

For certain embodiments, R₄is C_1-4alkyl.

For certain embodiments, R₄is hydrogen.

For certain embodiments, R₅is selected from the group consisting of:

For certain embodiments, R₅is

wherein R₈is hydrogen, A is —O—, —CH₂—, or —N(Q-R₄)—, and a and b are each 2.

For certain embodiments, R₅is

and R₇is propylene.

For certain embodiments, R₅is

wherein R₆is ═O, R₇is propylene, R₁₀is pentylene, A is —O—, and a and b are each 2.

For certain embodiments, R₆is selected from the group consisting of ═O and ═S.

For certain embodiments, R₆is ═O—.

For certain embodiments, R₆is ═S.

For certain embodiments, R₇is C_2-7alkylene.

For certain embodiments, R₇is C_2-4alkylene.

For certain embodiments, R₈is selected from the group consisting of hydrogen, alkyl, alkoxyalkylenyl, hydroxyalkylenyl, arylalkylenyl, and heteroarylalkylenyl.

For certain embodiments, R₈is selected from the group consisting of hydrogen, C_1-4alkyl, and C_1-4alkoxyC_1-4alkylenyl.

For certain embodiments, R₈is hydrogen or C_1-4alkyl.

For certain embodiments, R₈is selected from hydrogen and methyl

For certain embodiments, R₈is methyl.

For certain embodiments, R₈is hydrogen.

For certain embodiments, R₉is selected from the group consisting of hydrogen and alkyl.

For certain embodiments, R₁₀is C_3-8alkylene.

For certain embodiments, R₁₀is C_4-6alkylene.

For certain embodiments, R₁₀is pentylene.

For certain embodiments, A is selected from the group consisting of —O—, —C(O)—, —CH₂—, —S(O)_0-2—, and —N(Q-R₄)—.

For certain embodiments, A is —O—, —CH₂—, —S—, or —S(O)₂—.

For certain embodiments, A is —O—, —CH₂—, or —N(Q-R₄)—.

For certain embodiments, A is —O— or —S(O)₂—.

For certain embodiments, A is —O—.

For certain embodiments, A is —CH₂—.

For certain embodiments, A is —N(Q-R₄)—.

For certain embodiments, A is —N(CH₃)—.

For certain embodiments, A′ is selected from the group consisting of —O—, —S(O)_0-2—, —N(-Q-R₄)—, and —CH₂—.

For certain embodiments, including any one of the above embodiments of Formula II, G₁is selected from the group consisting of —C(O)—R′, α-aminoacyl, α-aminoacyl-α-aminoacyl, —C(O)—O—R′, —C(O)—N(R″)R′, —C(═NY′)—R′, —CH(OH)—C(O)—OY′, —CH(OC_1-4alkyl)Y₀, —CH₂Y₁, and —CH(CH₃)Y₁; R′ and R″ are independently selected from the group consisting of C_1-10alkyl, C_3-7cycloalkyl, phenyl, benzyl, and 2-phenylethyl, each of which may be unsubstituted or substituted by one or more substituents independently selected from the group consisting of halogen, hydroxy, nitro, cyano, carboxy, C_1-6alkyl, C_1-4alkoxy, aryl, heteroaryl, aryl-C_1-4alkylenyl, heteroaryl-C_1-4alkylenyl, halo-C_1-4alkylenyl, halo-C_1-4alkoxy, —O—C(O)—CH₃, —C(O)—O—CH₃, —C(O)—NH₂, —O—CH₂—C(O)—NH₂, —NH₂, and —S(O)₂—NH₂, with the proviso that R′ can also be hydrogen; α-aminoacyl is an α-aminoacyl group derived from an α-amino acid selected from the group consisting of racemic, D-, and L-amino acids; Y′ is selected from the group consisting of hydrogen, C_1-6alkyl, and benzyl; Y₀is selected from the group consisting of C_1-6alkyl, carboxy-C_1-6alkylenyl, amino-C_1-4alkylenyl, mono-N—C_1-6alkylamino-C_1-4alkylenyl, and di-N,N—C_1-6alkylamino-C_1-4alkylenyl; and Y₁is selected from the group consisting of mono-N—C_1-6alkylamino, di-N,N—C_1-6alkylamino, morpholin-4-yl, piperidin-1-yl, pyrrolidin-1-yl, and 4-C_1-4alkylpiperazin-1-yl.

For certain embodiments, including any one of the above embodiments of Formula II, G₁is selected from the group consisting of —C(O)—R′, α-aminoacyl, and —C(O)—O—R′. For certain of these embodiments, R′ contains one to ten carbon atoms. For certain of these embodiments, α-aminoacyl is an α-C_2-11aminoacyl group derived from an α-amino acid selected from the group consisting of racemic, D-, and L-amino acids containing a total of at least 2 carbon atoms and a total of up to 11 carbon atoms, and may also include one or more heteroatoms selected from the group consisting of O, S, and N.

For certain embodiments, including any one of the above embodiments of Formula III, G₂is selected from the group consisting of —X₂—C(O)—R′, α-aminoacyl, α-aminoacyl-α-aminoacyl, —X₂—C(O)—O—R′, and —C(O)—N(R″)R′. For certain of these embodiments, X₂is selected from the group consisting of a bond; —CH₂—O—; —CH(CH₃)—O—; —C(CH₃)₂—O—; and, in the case of —X₂—C(O)—O—R′, —CH₂—NH—; R′ and R″ are independently selected from the group consisting of C_1-10alkyl, C_3-7cycloalkyl, phenyl, benzyl, and 2-phenylethyl, each of which may be unsubstituted or substituted by one or more substituents independently selected from the group consisting of halogen, hydroxy, nitro, cyano, carboxy, C_1-6alkyl, C_1-4alkoxy, aryl, heteroaryl, aryl-C_1-4alkylenyl, heteroaryl-C_1-4alkylenyl, halo-C_1-4alkylenyl, halo-C_1-4alkoxy, —O—C(O)—CH₃, —C(O)—O—CH₃, —C(O)—NH₂, O—CH₂—C(O)—NH₂, —NH₂, and —S(O)₂—NH₂, with the proviso that R″ can also be hydrogen; and α-aminoacyl is an α-aminoacyl group derived from an α-amino acid selected from the group consisting of racemic, D-, and L-amino acids.

For certain embodiments, including any one of the above embodiments of Formula III, G₂is selected from the group consisting of —C(O)—R′ and α-aminoacyl, wherein R′ is C_1-6alkyl or phenyl which is unsubstituted or substituted by one or more substituents independently selected from the group consisting of halogen, hydroxy, nitro, cyano, carboxy, C_1-6alkyl, C_1-4alkoxy, aryl, heteroaryl, aryl-C_1-4alkylenyl, heteroaryl-C_1-4alkylenyl, halo-C_1-4alkylenyl, halo-C_1-4alkoxy, —O—C(O)—CH₃, —C(O)—O—CH₃, —C(O)—NH₂, —O—CH₂—C(O)—NH₂, —NH₂, and —S(O)₂—NH₂.

For certain embodiments, including any one of the above embodiments of Formula III, G₂is selected from the group consisting of α-amino-C_2-5alkanoyl, C_2-6alkanoyl, C_1-6alkoxycarbonyl, and C_1-6alkylcarbamoyl.

For certain embodiments, including any one of the above embodiments which include an α-aminoacyl group, α-aminoacyl is an α-aminoacyl group derived from a naturally occurring α-amino acid selected from the group consisting of racemic, D-, and L-amino acids.

For certain embodiments, including any one of the above embodiments which include an α-aminoacyl group, α-aminoacyl is an α-aminoacyl group derived from an α-amino acid found in proteins, wherein the amino acid is selected from the group consisting of racemic, D-, and L-amino acids.

For certain embodiments, the hydrogen atom of the hydroxy group of Formula II (including any one of its embodiments) is replaced by G₂, wherein G₂is defined as in any one of the above embodiments of G₂.

For certain embodiments, Q is selected from the group consisting of a bond, —C(R₆)—, —C(R₆)—C(R₆)—, —S(O)₂—, —C(R₆)—N(R₈)—W—, —S(O)₂—N(R₈)—, —C(R₆)—O—, —C(R₆)—S—, and —C(R₆)—N(OR₉)—.

For certain embodiments, Q is selected from the group consisting of a bond, —C(R₆)—, —S(O)₂, —C(R₆)—N(R₈)—, —S(O)₂—N(R₈)—, —C(R₆)—O—, and —C(R₆)—S—.

For certain embodiments, Q is selected from the group consisting of a bond, —C(R₆)—, —S(O)₂—, and —C(R₆)—N(R₈)—.

For certain embodiments, Q is selected from the group consisting of —C(O)—, —S(O)₂—, and —C(O)—N(R₈)—. In certain of these embodiments, R₈is hydrogen or methyl.

For certain embodiments, Q is selected from the group consisting of —S(O)₂—, —C(O)—, and —C(O)—NH—.

For certain embodiments, Q is —C(O)—.

For certain embodiments, Q is —S(O)₂—.

For certain embodiments, Q is —C(R₆)—N(R₈)—.

For certain embodiments, Q is —C(O)—N(R₈)— wherein R₈is hydrogen or methyl.

For certain embodiments, V is selected from the group consisting of —C(R₆)—, —O—C(R₆)—, —N(R₉)—C(R₆)—, and —S(O)₂—.

For certain embodiments, V is —C(R₆)—.

For certain embodiments, W is selected from the group consisting of a bond, —C(O)—, and —S(O)₂—.

For certain embodiments, W is a bond.

For certain embodiments, X is selected from the group consisting of alkylene, alkenylene, alkynylene, arylene, heteroarylene, and heterocyclylene wherein the alkylene, alkenylene, and alkynylene groups can be optionally interrupted or terminated by arylene, heteroarylene or heterocyclylene and optionally interrupted by one or more —O— groups.

For certain embodiments, X is alkylene that is optionally interrupted or terminated by heterocyclylene and optionally interrupted by one —O— group.

For certain embodiments, X is C_1-6alkylene which may be interrupted by one —O— group.

For certain embodiments, X is C_1-6alkylene.

For certain embodiments, X is C_2-6alkylene.

For certain embodiments, X is C_1-4alkylene.

For certain embodiments, X is phenylene.

For certain embodiments, X is methylene.

For certain embodiments, X is ethylene,

For certain embodiments, X is butylene.

For certain embodiments, Y is selected from the group consisting of —O—, —S(O)_0-2—, —S(O)₂—N(R₈)—, —C(R₆)—, —C(R₆)—O—, —O—C(R₆)—, —O—C(O)—O—, —N(R₈)-Q-, —C(R₆)—N(R₈)—, —O—C(R₆)—N(R₈)—, —C(R₆)—N(OR₈)—, —O—N(R₈)-Q-, —O—N═C(R₄)—, —C(═N—O—R₈)—,

For certain embodiments, Y is selected from the group consisting of —O—, —S(O)₂—,

For certain embodiments, Y is selected from the group consisting of —O—, —C(R₆)—,

For certain embodiments, Y is selected from the group consisting of —N(R₈)—C(O)—, —N(R₈)—S(O)₂—, —N(R₈)—C(O)—N(R₈)—, —N(R₈)—S(O)₂—N(R₈)—, —S(O)₂—, and

In certain of these embodiments, Q is —C(O)—, —C(O)—NH—, or S(O)₂—, R₁₀is pentylene, and R₈is hydrogen or methyl.

For certain embodiments, Y is selected from the group consisting of —N(R₈)—C(O)—, —N(R₈)—S(O)₂—, —N(R₈)—C(O)—N(R₈)—, and —S(O)₂—. In certain of these embodiments, R₈is selected from hydrogen and methyl.

For certain embodiments, Y is selected from the group consisting of —N(R₈)—C(O)—, —N(R₈)—S(O)₂—, and —N(R₈)—C(O)—N(R₈)— wherein R₈is selected from hydrogen and methyl.

For certain embodiments, Y is selected from the group consisting of —C(O)—, —C(O)—N(R₈)—, —N(R₈)—C(O)—, —N(R₈)—S(O)₂—, and —N(R₈)—C(O)—N(R₈)—. In certain of these embodiments, R₈is selected from hydrogen and methyl.

For certain embodiments, Y is

For certain of these embodiments, R₁₀is pentylene, and Q is selected from the group consisting of —S(O)₂—, —C(O)—, and —C(O)—NH—.

For certain embodiments, Y is —NH—S(O)₂—N(R₈)—. In certain of these embodiments, R₈is methyl.

For certain embodiments, Y¹is selected from the group consisting of —S—, —C(O)—, —C(O)—O—, —C(O)—N(R₈)—, —S(O)₂—N(R₈)—, and —N(R₈)—C(O)—.

For certain embodiments, Z is a bond or —O—.

For certain embodiments, Z is a bond.

For certain embodiments, Z is —O—.

For certain embodiments, a and b are independently integers from 1 to 6 with the proviso that a+b is ≦7.

For certain embodiments, a and b are each independently 1 to 3.

For certain embodiments, a and b are each 2.

For certain embodiments, a is 1, 2, or 3, and b is 2.

For certain embodiments, n is 1 or 2.

For certain embodiments, n is 1.

For certain embodiments, n is 2.

For certain embodiments, the compound, 1-[4-amino-2-hydroxymethyl-7-(thiazol-4-ylmethoxy)-1H-imidazo[4,5-c]quinolin-1-yl]-2-methylpropan-2-ol, pharmaceutically acceptable salts thereof, pharmaceutical compositions containing this compound or salt thereof in combination with a pharmaceutically acceptable carrier, and the use of this compound in the methods described herein are provided.

Preparation of the Compounds

Compounds of the invention may be synthesized by synthetic routes that include processes analogous to those well known in the chemical arts, particularly in light of the description contained herein. The starting materials are generally available from commercial sources such as Aldrich Chemicals (Milwaukee, Wis., USA) or are readily prepared using methods well known to those skilled in the art (e.g., prepared by methods generally described in Louis F. Fieser and Mary Fieser, Reagents for Organic Synthesis, v. 1-19, Wiley, New York, (1967-1999 ed.); Alan R. Katritsky, Otto Meth-Cohn, Charles W. Rees, Comprehensive Organic Functional Group Transformations, v. 1-6, Pergamon Press, Oxford, England, (1995); Barry M. Trost and Ian Fleming, Comprehensive Organic Synthesis, v. 1-8, Pergamon Press, Oxford, England, (1991); or Beilsteins Handbuch der organischen Chemie, 4, Aufl. Ed. Springer-Verlag, Berlin, Germany, including supplements (also available via the Beilstein online database)).

For illustrative purposes, the reaction schemes depicted below provide potential routes for synthesizing the compounds of the present invention as well as key intermediates. For more detailed description of the individual reaction steps, see the EXAMPLES section below. Those skilled in the art will appreciate that other synthetic routes may be used to synthesize the compounds of the invention. Although specific starting materials and reagents are depicted in the reaction schemes and discussed below, other starting materials and reagents can be easily substituted to provide a variety of derivatives and/or reaction conditions. In addition, many of the compounds prepared by the methods described below can be further modified in light of this disclosure using conventional methods well known to those skilled in the art.

In the preparation of compounds of the invention it may sometimes be necessary to protect a particular functionality while reacting other functional groups on an intermediate. The need for such protection will vary depending on the nature of the particular functional group and the conditions of the reaction step. Suitable amino protecting groups include acetyl, trifluoroacetyl, tert-butoxycarbonyl (Boc), benzyloxycarbonyl, and 9-fluorenylmethoxycarbonyl (Fmoc). Suitable hydroxy protecting groups include acetyl and silyl groups such as the tert-butyl dimethylsilyl group. For a general description of protecting groups and their use, see T. W. Greene and P. G. M. Wuts, Protective Groups in Organic Synthesis, John Wiley & Sons, New York, USA, 1991.

Conventional methods and techniques of separation and purification can be used to isolate compounds of the invention, as well as various intermediates related thereto. Such techniques may include, for example, all types of chromatography (high performance liquid chromatography (HPLC), column chromatography using common absorbents such as silica gel, and thin layer chromatography), recrystallization, and differential (i.e., liquid-liquid) extraction techniques.

In some embodiments, compounds of the invention can be prepared according to Reaction Scheme I, wherein R₁, R, m, and n are as defined above and allyl is methyl or ethyl.

In Reaction Scheme I an ether substituted 1H-imidazo[4,5-c]quinolin-4-amine of Formula X is cleaved to provide a hydroxyalkyl substituted 1H-imidazo[4,5-c]quinolin-4-amine of Formula I. The reaction is conveniently carried out by adding a solution of boron tribromide in a suitable solvent such as dichloromethane to a solution or suspension of a compound of Formula X in a suitable solvent such as dichloromethane at ambient or at a sub-ambient temperature, for example, at 0° C. The product or pharmaceutically acceptable salt thereof can be isolated using conventional methods.

Numerous compounds of Formula X are known; others can be prepared using known synthetic methods. See, for example, U.S. Pat. Nos. 6,069,149; 6,331,539; 6,451,810; 6,541,485; 6,756,382; 6,677,349; 6,573,273; 6,664,264; 6,664,265; 6,677,347; 6,660,735; 6,683,088; and 6,667,312 and the references cited therein.

In some embodiments, compounds of the invention can be prepared according to Reaction Scheme II, wherein R₁, G₁, and n are as defined above. Compounds of Formula I can be prepared according to the method described above. The amino group of a compound of Formula I can be converted by conventional methods to a functional group such as an amide, carbamate, urea, amidine, or another hydrolyzable group. A compound of this type can be made by the replacement of a hydrogen atom in an amino group with a group such as —C(O)—R′, α-aminoacyl, α-aminoacyl-α-aminoacyl, —C(O)—O—R′, —C(O)—N(R″)R′, —C(═NY′)—R′, —CH(OH)—C(O)—OY′, —CH(OC_1-4alkyl)Y₀, —CH₂Y₁, and —CH(CH₃)Y₁; wherein R′ and R″ are independently selected from the group consisting of C_1-10alkyl, C_3-7cycloalkyl, phenyl, benzyl, and 2-phenylethyl, each of which may be unsubstituted or substituted by one or more substituents independently selected from the group consisting of halogen, hydroxy, nitro, cyano, carboxy, C_1-6alkyl, C_1-4alkoxy, aryl, heteroaryl, aryl-C_1-4alkylenyl, heteroaryl-C_1-4alkylenyl, halo-C_1-4alkylenyl, halo-C_1-4alkoxy, —O—C(O)—CH₃, —C(O)—O—CH₃, —C(O)—NH₂, —O—CH₂—C(O)—NH₂, —NH₂, and —S(O)₂—NH₂, with the proviso that R″ can also be hydrogen; each α-aminoacyl is an α-aminoacyl group derived from an α-amino acid selected from the group consisting of racemic, D-, and L-amino acids; Y′ is selected from the group consisting of hydrogen, C_1-6alkyl, and benzyl; Y₀is selected from the group consisting of C_1-6alkyl, carboxy-C_1-6alkylenyl, amino-C_1-4alkylenyl, mono-N—C_1-6alkylamino-C_1-4alkylenyl, and di-N,N—C_1-6alkylamino-C_1-4alkylenyl; and Y₁is selected from the group consisting of mono-N—C_1-6alkylamino, di-N,N—C_1-6alkylamino, morpholin-4-yl, piperidin-1-yl, pyrrolidin-1-yl, and 4-C_1-4alkylpiperazin-1-yl. Particularly useful compounds of Formula II are amides derived from carboxylic acids containing one to ten carbon atoms, amides derived from amino acids, and carbamates containing one to ten carbon atoms. The reaction can be carried out, for example, by combining a compound of Formula I with a chloroformate or acid chloride, such as ethyl chloroformate or acetyl chloride, in the presence of a base such as triethylamine in a suitable solvent such as dichloromethane at ambient temperature.

Alternatively, the hydroxy group on a compound of Formula I can be protected using a suitable silyl group such as tert-butyl dimethylsilyl using conventional methods. The G₁group may then be installed using conventional methods followed by the removal of the hydroxy protecting group under acidic conditions to provide a compound of Formula II.

In some embodiments, compounds of the invention can be prepared according to Reaction Scheme III, wherein R₁, G₂, and n are as defined above. Compounds of Formula I can be prepared according to the method described above. The hydrogen atom of the alcohol group of a compound of Formula I can be replaced using conventional methods with a group such as X₂—C(O)—R′, α-aminoacyl, α-aminoacyl-α-aminoacyl, —X₂—C(O)—O—R′, and —C(O)—N(R″)R′; wherein X₂is selected from the group consisting of a bond; —CH₂—O—; —CH(CH₃)—O—; —C(CH₃)₂—O—; and, in the case of —X₂—C(O)—O—R′, —CH₂—NH—; R′ and R″ are independently selected from the group consisting of C_1-10alkyl, C_3-7cycloalkyl, phenyl, benzyl, and 2-phenylethyl, each of which may be unsubstituted or substituted by one or more substituents independently selected from the group consisting of halogen, hydroxy, nitro, cyano, carboxy, C_1-6alkyl, C_1-4alkoxy, aryl, heteroaryl, aryl-C_1-4alkylenyl, heteroaryl-C_1-4alkylenyl, halo-C_1-4alkylenyl, halo-C_1-4alkoxy, —O—C(O)—CH₃, —C(O)—O—CH₃, —C(O)—NH₂, —O—CH₂—C(O)—NH₂, —NH₂, and —S(O)₂—NH₂, with the proviso that R_Wcan also be hydrogen; and each α-aminoacyl is an α-aminoacyl group derived from an α-amino acid selected from the group consisting of racemic, D-, and L-amino acids. Particularly useful compounds of Formula III are esters made from carboxylic acids containing one to six carbon atoms, unsubstituted or substituted benzoic acid esters, or esters made from naturally occurring amino acids. For example, the reaction can be carried out by treating a compound of Formula I with a carboxylic acid or amino acid under Mitsunobu reaction conditions by adding triphenylphosphine and a carboxylic acid to a solution or suspension of a compound of Formula I in a suitable solvent such as tetrahydrofuran and then slowly adding diisopropyl azodicarboxylate. The reaction can be run at a sub-ambient temperature such as 0° C.

In some embodiments, compounds of the invention can also be prepared using the synthetic methods described in the EXAMPLES below.

Pharmaceutical Compositions and Biological Activity

Pharmaceutical compositions of the invention contain a therapeutically effective amount of a compound or salt described above in combination with a pharmaceutically acceptable carrier.

The terms “a therapeutically effective amount” and “effective amount” mean an amount of the compound or salt sufficient to induce a therapeutic or prophylactic effect, such as cytokine induction, immunomodulation, antitumor activity, and/or antiviral activity. Cytokine induction can include preferentially inducing the biosynthesis of IFN-α. The exact amount of compound or salt used in a pharmaceutical composition of the invention will vary according to factors known to those of skill in the art, such as the physical and chemical nature of the compound or salt, the nature of the carrier, and the intended dosing regimen.

In some embodiments, the compositions of the invention will contain sufficient active ingredient or prodrug to provide a dose of about 100 nanograms per kilogram (ng/kg) to about 50 milligrams per kilogram (mg/kg), preferably about 10 micrograms per kilogram (μg/kg) to about 5 mg/kg, of the compound or salt to the subject.

In other embodiments, the compositions of the invention will contain sufficient active ingredient or prodrug to provide a dose of, for example, from about 0.01 mg/m²to about 5.0 mg/m², computed according to the Dubois method, in which the body surface area of a subject (m²) is computed using the subject's body weight: m²=(wt kg^0.425×height cm^0.725)×0.007184, although in some embodiments the methods may be performed by administering a compound or salt or composition in a dose outside this range. In some of these embodiments, the method includes administering sufficient compound to provide a dose of from about 0.1 mg/m²to about 2.0 mg/m²to the subject, for example, a dose of from about 0.4 mg/m²to about 1.2 mg/m².

A variety of dosage forms may be used, such as tablets, lozenges, capsules, parenteral formulations (e.g., intravenous formulations), syrups, creams, ointments, aerosol formulations, transdermal patches, transmucosal patches and the like. These dosage forms can be prepared with conventional pharmaceutically acceptable carriers and additives using conventional methods, which generally include the step of bringing the active ingredient into association with the carrier.

The compounds or salts of the invention can be administered as the single therapeutic agent in the treatment regimen, or the compounds or salts described herein may be administered in combination with one another or with other active agents, including additional immune response modifiers, antivirals, antibiotics, antibodies, proteins, peptides, oligonucleotides, etc.

Compounds or salts of the invention have been shown to induce the production of certain cytokines in experiments performed according to the tests set forth below. These results indicate that the compounds or salts are useful for modulating the immune response in a number of different ways, rendering them useful in the treatment of a variety of disorders. The compounds or salts of the invention are especially useful as immune response modifiers due to their ability to preferentially induce interferon-α, thus providing a benefit over compounds that also induce pro-inflammatory cytokines (e.g. TNF-α) or that induce pro-inflammatory cytokines at higher levels. While interferon-α and pro-inflammatory cytokines are beneficial in treating certain conditions, interferon-α preferentially induced is believed to be better tolerated by patients, because the significantly lower levels of pro-inflammatory cytokines can result in fewer or less severe adverse side effects experienced by patients. For example, if a subject is treated for a disease (e.g., hepatitis C, metastatic cancer) with a compound that induces significant levels of pro-inflammatory cytokines, while treating the disease, the compound may also cause side effects, such as severe and/or widespread inflammation, tissue destruction, or emesis, that render the subject unable or unwilling to receive the treatment. Alternatively, if a subject is treated with a compound that preferentially induces interferon-α then the compound may treat the disease with less risk of adverse side effects from pro-inflammatory cytokines such as TNF-α. Therefore, by maintaining the ability to treat a condition and reducing adverse side effects, compounds that preferentially induce IFN-α provide an advantage over compounds that would also induce pro-inflammatory cytokines, such as TNF-α, at higher levels.

The ability of the compounds or salts of the invention to preferentially induce the biosynthesis of IFN-α may be particularly advantageous when administered systemically, since adverse side effects, including for example widespread inflammation, may be reduced or even eliminated. Compounds of the invention may be administered systemically in a number of ways, including but not limited to oral and intravenous administration.

Cytokines whose biosynthesis may be induced by compounds or salts of the invention include IFN-α, IP-10, MCP-1, and a variety of other cytokines. In some instances, cytokines such as TNF-α, IL-12 may be induced, albeit at significantly reduced levels. Among other effects, these and other cytokines can inhibit virus production and tumor cell growth, making the compounds or salts useful in the treatment of viral diseases and neoplastic diseases. Accordingly, the invention provides a method of inducing cytokine biosynthesis in an animal comprising administering an effective amount of a compound or salt of the invention to the animal. The animal to which the compound or salt is administered for induction of cytokine biosynthesis may have a disease as described infra, for example a viral disease or a neoplastic disease, and administration of the compound or salt may provide therapeutic treatment. Alternatively, the compound or salt may be administered to the animal prior to the animal acquiring the disease so that administration of the compound or salt may provide a prophylactic treatment.

In addition to the ability to induce the production of cytokines, compounds or salts of the invention can affect other aspects of the innate immune response. For example, the compounds or salts may cause maturation of dendritic cells or proliferation and differentiation of B-lymphocytes.

Whether for prophylaxis or therapeutic treatment of a disease, and whether for effecting innate or acquired immunity, the compound or salt or composition may be administered alone or in combination with one or more active components as in, for example, a vaccine adjuvant. When administered with other components, the compound or salt or composition and other component or components may be administered separately; together but independently such as in a solution; or together and associated with one another such as (a) covalently linked or (b) non-covalently associated, e.g., in a colloidal suspension.

Conditions for which compounds or salts or compositions identified herein may be used as treatments include, but are not limited to:

(a) viral diseases such as, for example, diseases resulting from infection by an adenovirus, a herpesvirus (e.g., HSV-I, HSV-II, CMV, or VZV), a poxvirus (e.g., an orthopoxvirus such as variola or vaccinia, or molluscum contagiosum), a picornavirus (e.g., rhinovirus or enterovirus), an orthomyxovirus (e.g., influenzavirus), a paramyxovirus (e.g., parainfluenzavirus, mumps virus, measles virus, and respiratory syncytial virus (RSV)), a coronavirus (e.g., SARS), a papovavirus (e.g., papillomaviruses, such as those that cause genital warts, common warts, or plantar warts), a hepadnavirus (e.g., hepatitis B virus), a flavivirus (e.g., hepatitis C virus or Dengue virus), or a retrovirus (e.g., a lentivirus such as HIV);

(b) bacterial diseases such as, for example, diseases resulting from infection by bacteria of, for example, the genus Escherichia, Enterobacter, Salmonella, Staphylococcus, Shigella, Listeria, Aerobacter, Helicobacter, Klebsiella, Proteus, Pseudomonas, Streptococcus, Chlamydia, Mycoplasma, Pneumococcus, Neisseria, Clostridium, Bacillus, Corynebacterium, Mycobacterium, Campylobacter, Vibrio, Serratia, Providencia, Chromobacterium, Brucella, Yersinia, Haemophilus, or Bordetella;

(c) other infectious diseases, such as chlamydia, fungal diseases including but not limited to candidiasis, aspergillosis, histoplasmosis, cryptococcal meningitis, or parasitic diseases including but not limited to malaria, pneumocystis carnii pneumonia, leishmaniasis, cryptosporidiosis, toxoplasmosis, and trypanosome infection;

(d) neoplastic diseases, such as intraepithelial neoplasias, cervical dysplasia, actinic keratosis, basal cell carcinoma, squamous cell carcinoma, renal cell carcinoma, Kaposi's sarcoma, melanoma, leukemias including but not limited to acute myeloid leukemia, acute lymphocytic leukemia, chronic myeloid leukemia, chronic lymphocytic leukemia, multiple myeloma, Hodgkin's lymphoma, non-Hodgkin's lymphoma, cutaneous T-cell lymphoma, B-cell lymphoma, and hairy cell leukemia, and other cancers;

(e) T_H2-mediated, atopic diseases, such as atopic dermatitis or eczema, eosinophilia, asthma, allergy, allergic rhinitis, and Ommen's syndrome;

(f) certain autoimmune diseases such as systemic lupus erythematosus, essential thrombocythaemia, multiple sclerosis, discoid lupus, alopecia areata; and

(g) diseases associated with wound repair such as, for example, inhibition of keloid formation and other types of scarring (e.g., enhancing wound healing, including chronic wounds).

Additionally, a compound or salt identified herein may be useful as a vaccine adjuvant for use in conjunction with any material that raises either humoral and/or cell mediated immune response, such as, for example, live viral, bacterial, or parasitic immunogens; inactivated viral, tumor-derived, protozoal, organism-derived, fungal, or bacterial immunogens; toxoids; toxins; self-antigens; polysaccharides; proteins; glycoproteins; peptides; cellular vaccines; DNA vaccines; autologous vaccines; recombinant proteins; and the like, for use in connection with, for example, BCG, cholera, plague, typhoid, hepatitis A, hepatitis B, hepatitis C, influenza A, influenza B, parainfluenza, polio, rabies, measles, mumps, rubella, yellow fever, tetanus, diphtheria, hemophilus influenza b, tuberculosis, meningococcal and pneumococcal vaccines, adenovirus, HIV, chicken pox, cytomegalovirus, dengue, feline leukemia, fowl plague, HSV-1 and HSV-2, hog cholera, Japanese encephalitis, respiratory syncytial virus, rotavirus, papilloma virus, yellow fever, and Alzheimer's Disease.

Compounds or salts identified herein may be particularly helpful in individuals having compromised immune function. For example, compounds or salts may be used for treating the opportunistic infections and tumors that occur after suppression of cell mediated immunity in, for example, transplant patients, cancer patients and HIV patients.

Thus, one or more of the above diseases or types of diseases, for example, a viral disease or a neoplastic disease may be treated in an animal in need thereof (having the disease) by administering a therapeutically effective amount of a compound or salt of the invention to the animal.

An animal may also be vaccinated by administering an effective amount of a compound or salt described herein, as a vaccine adjuvant. In one embodiment, there is provided a method of vaccinating an animal comprising administering an effective amount of a compound or salt described herein to the animal as a vaccine adjuvant.

An amount of a compound or salt effective to induce cytokine biosynthesis is an amount sufficient to cause one or more cell types, such as dendritic cells and B-cells to produce an amount of one or more cytokines such as, for example, IFN-α, IP-10, and MCP-1 that is increased (induced) over a background level of such cytokines. The precise amount will vary according to factors known in the art but is expected to be a dose of about 100 ng/kg to about 50 mg/kg, preferably about 10 μg/kg to about 5 mg/kg. In other embodiments, the amount is expected to be a dose of, for example, from about 0.01 mg/m²to about 5.0 mg/m², (computed according to the Dubois method as described above) although in some embodiments the induction of cytokine biosynthesis may be performed by administering a compound or salt in a dose outside this range. In some of these embodiments, the method includes administering sufficient compound or salt or composition to provide a dose of from about 0.1 mg/m²to about 2.0 mg/m²to the subject, for example, a dose of from about 0.4 mg/m²to about 1.2 mg/m².

The invention provides a method of treating a disease which is responsive to the induction of cytokine biosynthesis, particularly the preferential induction of IFN-α, including a method of treating a viral infection in an animal and a method of treating a neoplastic disease in an animal, comprising administering an effective amount of a compound or salt or composition of the invention to the animal. An amount effective to treat or inhibit a viral infection is an amount that will cause a reduction in one or more of the manifestations of viral infection, such as viral lesions, viral load, rate of virus production, and mortality as compared to untreated control animals. The precise amount that is effective for such treatment will vary according to factors known in the art but is expected to be a dose of about 100 ng/kg to about 50 mg/kg, preferably about 10 μg/kg to about 5 mg/kg. An amount of a compound or salt effective to treat a neoplastic condition is an amount that will cause a reduction in tumor size or in the number of tumor foci. Again, the precise amount will vary according to factors known in the art but is expected to be a dose of about 100 ng/kg to about 50 mg/kg, preferably about 10 μg/kg to about 5 mg/kg. In other embodiments, the amount is expected to be a dose of, for example, from about 0.01 mg/m²to about 5.0 mg/m², (computed according to the Dubois method as described above) although in some embodiments either of these methods may be performed by administering a compound or salt in a dose outside this range. In some of these embodiments, the method includes administering sufficient compound or salt to provide a dose of from about 0.1 mg/m²to about 2.0 mg/m²to the subject, for example, a dose of from about 0.4 mg/m²to about 1.2 mg/m².

In addition to the formulations and uses described specifically herein, other formulations, uses, and administration devices suitable for compounds of the present invention are described in, for example, International Publication Nos. WO 03/077944 and WO 02/036592, U.S. Pat. No. 6,245,776, and U.S. Publication Nos. 2003/0139364, 2003/185835, 2004/0258698, 2004/0265351, 2004/076633, and 2005/0009858.

Objects and advantages of this invention are further illustrated by the following examples, but the particular materials and amounts thereof recited in these examples, as well as other conditions and details, should not be construed to unduly limit this invention.

EXAMPLES

In the examples below normal high performance flash chromatography (prep HPLC) was carried out using a COMBIFLASH system (an automated high-performance flash purification product available from Teledyne Isco, Inc., Lincoln, Nebr., USA) or a HORIZON HPFC system (an automated high-performance flash purification product available from Biotage, Inc, Charlottesville, Va., USA). The eluent used for each purification is given in the example. In some chromatographic separations, the solvent mixture 80/18/2 v/v/v chloroform/methanol/concentrated ammonium hydroxide (CMA) was used as the polar component of the eluent. In these separations, CMA was mixed with chloroform in the indicated ratio.

Example 1
N-{3-[4-Amino-2-(2-hydroxyethyl)-1H-imidazo[4,5-c]quinolin-1-yl]propyl}-4-methylbenzenesulfonamide

Boron tribromide (5.50 mL of 1 M in dichloromethane) was added dropwise to a chilled (0° C.) suspension of N-{3-[4-amino-2-(2-methoxyethyl)-1H-imidazo[4,5-c]quinolin-1-yl]propyl}-4-methylbenzenesulfonamide (1.0 g, 2.2 mmol; U.S. Pat. No. 6,677,349, Example 253) in dichloromethane (20 mL). The reaction mixture was stirred at 0° C. for 3 hours. The reaction mixture was quenched with methanol. Hydrochloric acid (about 10 mL of 6 N) was added and the mixture was stirred at 50° C. overnight. The mixture was diluted with water (50 mL) and ethyl acetate (100 mL) and then brought to neutral pH with solid sodium hydroxide. The layers were separated and the aqueous was extracted with ethyl acetate (×2). The combined organics were dried over magnesium sulfate, filtered, and then concentrated under reduced pressure to provide a yellow solid. This material was purified by prep HPLC (COMBIFLASH system eluting first with a gradient of 0 to 5% methanol in dichloromethane containing 1% ammonium hydroxide and then with a gradient of 5 to 10% methanol in dichloromethane containing 1% ammonium hydroxide) to provide a white solid. This material was suspended in hot acetonitrile, allowed to cool, and then the solvent was decanted. The resulting material was dried under vacuum to provide about 200 mg of N-{3-[4-amino-2-(2-hydroxyethyl)-1H-imidazo[4,5-c]quinolin-1-yl]propyl}-4-methylbenzenesulfonamide as a white solid, m.p. 231-232° C. Anal, calcd for C₂₂H₂₅N₅O₃S.0.20 CH₄O: % C, 59.79; % H, 5.85; % N, 15.70. Found: % C, 59.44; % H, 5.89; % N, 15.52.

Example 2
N-{3-[4-Amino-2-(2-hydroxyethyl)-1H-imidazo[4,5-c]quinolin-1-yl]propyl}isoquinoline-3-carboxamide

Boron tribromide (5.50 mL of 1 M in dichloromethane) was added dropwise to a chilled (0° C.) suspension of N-{3-[4-amino-2-(2-methoxyethyl)-1H-imidazo[4,5-c]quinolin-1-yl]propyl}isoquinoline-3-carboxamide (1.0 g, 2.2 mmol; U.S. Pat. No. 6,756,382, Example 192) in dichloromethane (20 mL). The reaction mixture was stirred at 0° C. for 45 minutes and then allowed to warm to ambient temperature. After 5 hours the reaction mixture was concentrated under reduced pressure and the residue was allowed to stand over the weekend. The residue was diluted with methanol (20 mL) and then heated to 50° C. Hydrochloric acid (about 10 mL of 6 N) was added and the mixture was stirred for about 2.5 hours. The mixture was made basic with aqueous sodium hydroxide and then extracted with ethyl acetate (×2). The combined extracts were dried over magnesium sulfate, filtered, and then concentrated under reduced pressure to provide a yellow solid. This material was purified by prep HPLC (COMBIFLASH system eluting first with a gradient of 0 to 5% methanol in dichloromethane containing 1% ammonium hydroxide and then with a gradient of 5 to 10% methanol in dichloromethane containing 1% ammonium hydroxide) to provide a white solid. This material was suspended in hot acetonitrile, allowed to cool, and then the solvent was decanted. The resulting material was dried under vacuum to provide about 400 mg of N-{3-[4-amino-2-(2-hydroxyethyl)-1H-imidazo[4,5-c]quinolin-1-yl]propyl}isoquinoline-3-carboxamide as a white solid, mp 245-246° C. Anal calcd for C₂₅H₂₄N₆O₂: % C, 67.73; % H, 5.59; % N, 18.80. Found: % C, 67.38; % H, 5.54; % N, 18.84.

Example 3
N-{4-[4-Amino-2-(2-hydroxyethyl)-1H-imidazo[4,5-c]quinolin 1-yl]butyl}methanesulfonamide

Part A

3-Methoxypropionyl chloride (15.4 g, 126 mmol) was added dropwise over a period of 20 minutes to a chilled (ice bath) solution of tert-butyl N-{4-[(3-aminoquinolin-4-yl)amino]butyl}carbamate (38 g, 115 mmol, U.S. Pat. No. 6,541,485, Example 2, Part B) in pyridine. The reaction mixture was stirred for 4 hours and then allowed to stand at ambient temperature over the weekend. Pyridine hydrochloride (3.9 g, 34 mmol) was added and the reaction mixture was heated at reflux overnight. The reaction mixture was concentrated under reduced pressure and the residue was diluted with dichloromethane (250 mL) and aqueous sodium bicarbonate (250 mL). The layers were separated. The separatory funnel was rinsed with a small amount of methanol to remove a residue coating the walls. The combined organics were concentrated under reduced pressure. The residue was purified by prep HPLC (COMBIFLASH system eluting first with a gradient of 0 to 5% methanol in dichloromethane containing 1% ammonium hydroxide and then with a gradient of 5 to 10% methanol in dichloromethane containing 1% ammonium hydroxide) to provide 18 g of tert-butyl N-{4-[2-(2-methoxyethyl)-1H-imidazo[4,5-c]quinolin-1-yl]butyl}carbamate.

Part B

3-Chloroperoxybenzoic acid (20 g of 77%) was added in a single portion to a solution of the material from Part A (18 g, 45.2 mmol) in dichloroethane (170 mL). After 2 hours concentrated ammonium hydroxide (150 mL) was added and the reaction mixture was stirred until the phases were mixed well. Para-Toluenesulfonyl chloride (10.6 g, 54 mmol) was added in a single portion along with a small amount of dichloroethane. The reaction mixture was stirred overnight at ambient temperature and then diluted with water and dichloromethane. The layers were separated and the aqueous layer was extracted with dichloromethane (×2). The combined organics were dried over magnesium sulfate, filtered, and then concentrated under reduced pressure to provide 23 g of crude tert-butyl N-{4-[4-amino-2-(2-methoxyethyl)-1H-imidazo[4,5-c]quinolin-1-yl]butyl}carbamate as a red tar.

Part C

The material from Part B was combined with a solution of hydrochloric acid in dioxane (325 mL of 4 M) and stirred at ambient temperature for 3 hours. The reaction mixture was concentrated under reduced pressure. The residue was dissolved in methanol (30 mL) and 6 M sodium hydroxide was added with stirring to about pH 9. Attempts to extract with dichloromethane and ethyl acetate were not successful. The organic and aqueous layers were concentrated under reduced pressure and combined to provide a dark orange solid. This material was purified by prep HPLC (COMBIFLASH system eluting first with a gradient of 0 to 8% methanol in dichloromethane containing 1% ammonium hydroxide and then with a gradient of 9 to 35% methanol in dichloromethane containing 1% ammonium hydroxide) to provide 10.65 g of 1-(4-aminobutyl)-2-(2-methoxyethyl)-1H-imidazo[4,5-c]quinolin-4-amine as an orange solid.

Part D

Triethylamine (10.5 mL, 75.0 mmol) was added to a mixture of a portion (4.7 g, 15 mmol) of the material from Part C in pyridine (50 mL). The reaction mixture was stirred for several minutes and then methanesulfonyl chloride (1.27 mL, 16.5 mmol) was added dropwise. The reaction mixture was stirred at ambient temperature for 2 hours and then at 50° C. for 2 hours. More methanesulfonyl chloride (0.5 eq) was added and the reaction mixture was stirred at 50° C. for 2 hours. Another portion of methanesulfonyl chloride (0.25 eq) was added and the reaction mixture was stirred at ambient temperature overnight. The reaction mixture was diluted with dichloromethane and water. The layers were separated and the aqueous layer was extracted with dichloromethane (×3). The combined organics were dried over magnesium sulfate, filtered, and then concentrated under reduced pressure to provide 5 g of crude N-{4-[4-amino-2-(2-methoxyethyl)-1H-imidazo[4,5-c]quinolin-1-yl]butyl}methanesulfonamide as a red oil.

Part E

Boron tribromide (22.4 mL of 1 M in dichloromethane) was added slowly to a chilled (ice bath) mixture of a portion of the material from Part D (3.5 g, about 8.9 mmol) and dichloromethane (50 mL). After the addition was complete the ice bath was removed and the reaction mixture was allowed to stir at ambient temperature for 3 hours. The reaction mixture was concentrated under reduced pressure. The residue was dissolved in methanol and then combined with hydrochloric acid (50 mL of 6 M). The mixture was stirred at 50° C. for 2 hours and then concentrated under reduced pressure. The residue was combined with ammonia in methanol (about 50 mL of 7 M) to neutralize the acid and then concentrated. This procedure was repeated 3 times. The crude product was purified by prep HPLC (COMBIFLASH system eluting with a gradient of 0 to 10% methanol in dichloromethane containing 1% ammonium hydroxide). The product was stirred with hot acetonitrile, allowed to stand overnight, and then isolated by filtration, washed with acetonitrile, and dried in a vacuum oven to provide 1.1 g of N-{4-[4-amino-2-(2-hydroxyethyl)-1H-imidazo[4,5-c]quinolin-1-yl]butyl}methanesulfonamide, mp 206-208° C. Anal calcd for C₁₇H₂₃N₅O₃S: % C, 54.09; % H, 6.14; % N, 18.55. Found: % C, 53.83; % H, 6.29; % N, 18.29.

Example 4
1-(2-Amino-2-methylpropyl)-2-hydroxymethyl-1H-imidazo[4,5-c]quinolin-4-amine

Part A

Under a nitrogen atmosphere, triethylamine (6.6 mL, 47 mmol) was added slowly to a solution of 2,4-dichloro-3-nitroquinoline (10.0 g, 41.1 mmol) in anhydrous 1-methyl-2-pyrrolidinone (40 mL). The reaction mixture was cooled to 0° C. with an ice bath. A solution of 1,2-diamino-2-methylpropane (4.1 g, 47.3 mmol) in anhydrous 1-methyl-2-pyrrolidinone (5 mL) was added dropwise over a period of 15 minutes while maintaining the temperature of the reaction mixture below 4° C. After the addition was completed the ice bath was removed and the reaction mixture was allowed to stir at ambient temperature for 4 hours. The reaction mixture was slowly poured into vigorously stirred warm water (300 mL). The resulting suspension was stirred for 1 hour and then cooled to 13° C. by adding ice. The solid was isolated by filtration and then washed with cold water until the filtrate was clear to provide 12.1 g of N¹-(2-chloro-3-nitroquinolin-4-yl)-2-methylpropane-1,2-diamine as a damp yellow solid.

Part B

A solution of sodium hydroxide (1.8 g of solid sodium hydroxide dissolved in 45 mL of water) was added slowly to a solution of the material from Part A (41.1 mmol) in tetrahydrofuran (96 mL). A solution of di-tert-butyl dicarbonate (10.8 g, 49.4 mmol) in tetrahydrofuran (30 mL) was added dropwise over a period of 15 minutes. The reaction solution was stirred at ambient temperature. After 6 hours 10% sodium hydroxide (2 mL) and additional di-tert-butyl dicarbonate (1.5 g) were added and the reaction solution was stirred at ambient temperature overnight. The layers were separated and the tetrahydrofuran was removed under reduced pressure to provide a mixture. The mixture was diluted with water (200 mL) and then extracted with dichloromethane (2×100 mL). The organics were combined, washed sequentially with aqueous sodium carbonate (2×150 mL) and brine (100 mL), dried over sodium sulfate and magnesium sulfate, filtered, and then concentrated under reduced pressure. The residue was triturated with heptane (75 mL) for 15 minutes at 65° C. and then filtered while hot. The isolated solids were washed with heptane (20 mL) to provide 13.2 g of tert-butyl N-{2-[(2-chloro-3-nitroquinolin-4-yl)amino]-1,1-dimethylethyl}carbamate as a yellow powdery solid.

Part C

A Parr vessel was charged with 5% Pt/C (0.5 g) and acetonitrile (10 mL). A solution of the material from Part B in acetonitrile (450 mL) was added. The vessel was placed on a Parr shaker under hydrogen pressure (40 psi, 2.8×10⁵Pa) for 5 hours. The reaction mixture was filtered through a layer of CELITE filter aid to remove the catalyst. The filtrate was carried on to the next step.

Part D

The solution of tert-butyl N-{2-[(3-amino-2-chloroquinolin-4-yl)amino]-1,1-dimethylethyl}carbamate in acetonitrile from Part C was cooled to 5° C. using an ice bath. A solution of acetoxyacetyl chloride (4.8 g, 35.1 mmol) in acetonitrile (20 mL) was added dropwise at a rate such that the temperature of the reaction mixture was maintained at 5° C. After the addition was complete the ice bath was removed and the reaction mixture was allowed to stir at ambient temperature for 5 hours. The reaction mixture was concentrated under reduced pressure to provide 16.7 g of N-{2-[(3-acetoxyacetylamino-2-chloroquinolin-4-yl)amino]-1,1-dimethylethyl}carbamate hydrochloride as a yellow powder.

Part E

A mixture of the material from Part D (15.7 g) and ammonia in methanol (235 mL of 7 N) was divided into equal portions and placed in pressure vessels. The vessels were sealed, heated at 160° C. for 20 hrs, and then allowed to cool to ambient temperature overnight. The reaction mixtures were filtered. The isolated solids were washed with water and dried in a vacuum oven at 60° C. overnight to provide 6.0 g of a tan powder. A portion (1 g) was treated with activated charcoal and recrystallized from ethanol (75 mL) to provide 0.5 g of 1-(2-amino-2-methylpropyl)-2-hydroxymethyl-1H-imidazo[4,5-c]quinolin-4-amine as a white granular solid, mp 248-250° C. Anal calcd for C₁₅H₁₉N₅O: % C, 63.14; % H, 6.71; % N, 24.54. Found: % C, 63.13; % H, 6.81; % N, 24.64.

Example 5
N-[2-(4-Amino-2-hydroxymethyl-1H-imidazo[4,5-c]quinolin-1-yl)-1,1-dimethylethyl]cyclohexanecarboxamide

A solution of 1-(2-amino-2-methylpropyl)-2-hydroxymethyl-1H-imidazo[4,5-c]quinolin-4-amine (2.0 g, 7.0 mmol) in 1-methyl-2-pyrrolidinone (30 mL) was cooled to −20° C. Triethylamine (1.1 mL, 7.7 mmol) was added in a single portion. A chilled (−5° C.) solution of cyclohexanecarbonyl chloride (1.03 g, 7.0 mmol) in 1-methyl-2-pyrrolidinone (2 mL) was added dropwise over a period of 20 minutes while maintaining the reaction mixture at −20° C. The reaction mixture was stirred at ambient temperature overnight. Additional cyclohexanecarbonyl chloride (0.1 g) was added and the reaction mixture stirred for 2 hours. The reaction mixture was poured into water with vigorous stirring. The resulting precipitate was isolated by filtration to provide 1.7 g of an ivory powder. Analysis by high performance liquid chromatography and NMR indicated that the powder was a mixture of the desired product and an ester formed from the reaction of the hydroxy group of the desired product with cyclohexanecarbonyl chloride.

The powder was dissolved in ethanol (25 mL), combined with a solution of sodium hydroxide (0.21 g) in water (25 mL), and then heated at 50° C. for 3 hours. The ethanol was removed under reduced pressure and the solids were isolated by filtration to provide 1.2 g of a light tan powder. The powder was dissolved in a mixture of acetonitrile (100 mL), water (2 mL) and ethanol (25 mL). The solution was allowed to stand overnight and was then concentrated to a volume of 5 mL to provide a white paste. The paste was triturated with warm (70° C.) acetonitrile (50 mL) for 30 minutes, heated to reflux, and then allowed to cool to ambient temperature. The resulting solid was isolated by filtration to provide 1.05 g of N-[2-(4-amino-2-hydroxymethyl-1H-imidazo[4,5-c]quinolin-1-yl)-1,1-dimethylethyl]cyclohexanecarboxamide as a light yellow powder, mp 248-250° C. Anal calcd for C₂₂H₂₉N₅O₂: % C, 66.81; % H, 7.39; % N, 17.71. Found: % C, 66.56; % H, 7.60; % N, 17.82.

Example 6
N-{2-[4-Amino-2-(2-hydroxyethyl)-1H-imidazo[4,5-c]quinolin-1-yl]-1,1-dimethylethyl}methanesulfonamide

Part A

Triethylamine (39.3 mL, 0.282 mol) was added to a chilled (ice bath) solution of N¹-(2-chloro-3-nitroquinolin-4-yl)-2-methylpropane-1,2-diamine (41.42 g, 0.141 mol) in dichloromethane (about 500 mL). Under a nitrogen atmosphere a solution of methanesulfonic anhydride in (29.47 g, 0.169 mol) in dichloromethane (100 mL) was added via a cannula to the reaction mixture over a period of 45 minutes. After the addition was complete the ice bath was removed and the reaction mixture was allowed to stir at ambient temperature overnight. The reaction mixture was washed sequentially with saturated aqueous sodium bicarbonate (×2) and brine, dried over a mixture of sodium sulfate and magnesium sulfate, filtered, and then concentrated under reduced pressure to provide 46.22 g of an orange solid. This material was recrystallized from toluene (about 1 L), isolated by filtration, rinsed with cold toluene, and dried under high vacuum at 60° C. to provide 33.09 g of N-{2-[(2-chloro-3-nitroquinolin-4-yl)amino]-1,1-dimethylethyl}methanesulfonamide.

Part B

A hydrogenation vessel was charged with 5% Pt/C (4.14 g) and a solution of N-{2-[(2-chloro-3-nitroquinolin-4-yl)amino]-1,1-dimethylethyl}methanesulfonamide (54.59 g, 0.147 mol) in acetonitrile (1800 mL). The vessel was placed under hydrogen pressure (48 psi, 3.3×10⁵Pa) overnight. An additional portion (4.25 g) of catalyst was added and the vessel was placed under hydrogen pressure (48 psi, 3.3×10⁵Pa) for 4 hours. The reaction mixture was filtered through a layer of CELITE filter aid and the filter cake was rinsed with fresh acetonitrile until the washes were clear.

Part C

Under a nitrogen atmosphere, 3-methoxypropionyl chloride (17.6 mL, 0.162 mol) was added dropwise to the solution of N-{2-[(3-amino-2-chloroquinolin-4-yl)amino]-1,1-dimethylethyl}methanesulfonamide (0.147 mol) in acetonitrile (2.2 L) from Part B. The reaction mixture was allowed to stir at ambient temperature over the weekend. The resulting precipitate was isolated by filtration, rinsed with a small amount of acetonitrile, and then dried under high vacuum at 60° C. to provide 55.84 g of N-{2-chloro-4-[2-(methanesulfonylamino)-2-methylpropyl]quinolin-3-yl}-3-methoxypropionamide.

Part D

A Parr bomb was charged with 25.0 g of N-{2-chloro-4-[2-(methanesulfonylamino)-2-methylpropyl]aminoquinolin-3-yl}-3-methoxypropionamide and ammonia in methanol (300 mL of 7 N). A second vessel was charged with 30.21 g of N-{2-chloro-4-[2-(methanesulfonylamino)-2-methylpropyl]quinolin-3-yl}-3-methoxypropionamide and ammonia in methanol (400 mL of 7 N). Both vessels were sealed and then heated at 170° C. for 14 hours. The reaction mixtures were combined and the solvent was removed under reduced pressure. The residue was partitioned between dichloromethane and saturated aqueous sodium bicarbonate. The organic layer was washed sequentially with saturated aqueous sodium bicarbonate and brine, dried over sodium sulfate, filtered, and then concentrated under reduced pressure to provide 38.16 g of N-{2-[4-amino-2-(2-methoxyethyl)-1H-imidazo[4,5-c]quinolin-1-yl]-1,1-dimethylethyl}methanesulfonamide as an off white foam.

Part E

Under a nitrogen atmosphere, boron tribromide (3.5 mL of 1 M in dichloromethane) was added dropwise to a chilled (0° C.) solution of N-{2-[4-amino-2-(2-methoxyethyl)-1H-imidazo[4,5-c]quinolin-1-yl]-1,1-dimethylethyl}methanesulfonamide (0.55 g, 1.40 mmol) in dichloromethane (20 mL). The reaction was allowed to warm to ambient temperature overnight. The reaction was quenched with methanol (10 mL) and the solvent was removed under reduced pressure. The residue was dissolved in hydrochloric acid (6 N), stirred at 50° C. for about 2.5 hours, and then allowed to cool to ambient temperature. The reaction mixture was adjusted to pH 11 with ammonium hydroxide and then extracted with dichloromethane (×10). The combined organics were washed with brine, dried over sodium sulfate, filtered, and then concentrated under reduced pressure to provide 0.47 g of a white solid. This material was purified by prep HPLC (HORIZON HPFC system, eluting with a gradient of 30-50% CMA in chloroform for 15 column volumes followed by 50% CMA in chloroform for 5 column volumes) and then dried under high vacuum to provide 250 mg of N-{2-[4-amino-2-(2-hydroxyethyl)-1H-imidazo[4,5-c]quinolin-1-yl]-1,1-dimethylethyl}methanesulfonamide as white solid, m.p. 209-212° C. ¹H NMR (500 MHz, DMSO-d₆) δ 8.30 (d, J=8.2 Hz, 1H), 7.60 (d, J=8.2 Hz, 1H), 7.39 (m, 1H), 7.27 (s, 1H), 7.21 (m, 1H), 6.49 (s, 2H), 4.84 (t, J=5.4 Hz, 2H), 4.82 (br s, 1H), 3.88 (m, 2H), 3.18 (br s, 2H), 3.00 (s, 3H), 1.27 (br s, 6H); ¹³C NMR (125 MHz, DMSO-d₆) δ 153.6, 152.0, 145.4, 133.5, 126.9, 126.8, 126.5, 121.3, 120.8, 115.6, 60.5, 57.9, 54.1, 44.8, 31.4, 25.8; MS (ESI) m/z 378 (M+H)⁺; Anal. calcd for C₁₇H₂₃N₅O₃S: % C, 54.09; % H, 6.14; % N, 18.55. Found: % C, 53.76; % H, 6.02; % N, 18.32.

Example 7
N-[2-(4-Amino-2-hydroxymethyl-1H-imidazo[4,5-c]quinolin-1-yl)-1,1-dimethylethyl]methanesulfonamide

Part A

A pressure vessel was charged with a solution of N-{2-[(2-chloro-3-nitroquinolin-4-yl)amino]-1,1-dimethylethyl}methanesulfonamide (5 g, 13 mmol) in acetonitrile (150 mL). Catalyst was added (0.5 g of 5% Pt/C) and the vessel was placed under hydrogen pressure (50 psi, 3.4×10⁵Pa) for 2 hours. The reaction mixture was filtered through a layer of CELITE filter aid.

Part B

The solution of N-{2-[(3-amino-2-chloroquinolin-4-yl)amino]-1,1-dimethylethyl}methanesulfonamide in acetonitrile from Part A was chilled in an ice bath. Acetoxyacetyl chloride (1.5 mL, 14 mmol) was added over a period of 5 minutes. The reaction mixture was allowed to stir for 3 hours. A precipitate was isolated by filtration and rinsed with acetonitrile to provide crude N-{2-chloro-4-[2-(methanesulfonylamino)-2-methylpropyl]quinolin-3-yl}acetoxyacetamide hydrochloride.

Part C

A solution of sodium hydroxide (0.8 g) in water (15 mL) was added to a suspension of the material from Part B in ethanol (60 mL) until all of the solid dissolved. The reaction mixture was heated at 60° C. overnight and then concentrated under reduced pressure. The residue was dissolved in water (50 mL), sodium chloride (10 g) was added, and the mixture was extracted with chloroform (3×300 mL). The extracts were concentrated under reduced pressure to provide about 4 g of crude N-[2-(4-chloro-2-hydroxymethyl-1H-imidazo[4,5-c]quinolin-1-yl)-1,1-dimethylethyl]methanesulfonamide.

Part D

The material from Part C was combined with a solution of ammonia in methanol (50 mL of 7 N) and heated at 150° C. for 10 hours. The reaction mixture was allowed to cool to ambient temperature. A precipitate was isolated by filtration, rinsed with methanol (20 mL), slurried with water (50 mL), isolated by filtration, washed with water (20 mL), and dried to provide 2.7 g of a brown crystalline solid. This material was combined with methanol (50 mL), heated at 50° C. overnight, and then isolated by filtration to provide 2.3 g of N-[2-(4-amino-2-hydroxymethyl-1H-imidazo[4,5-c]quinolin-1-yl)-1,1-dimethylethyl]methanesulfonamide, mp 262-265° C. Anal. calcd for C₁₆H₂₁N₅O₃S: % C, 52.88; % H, 5.82; % N, 19.27. Found: % C, 52.64; % H, 5.95; % N, 19.50.

Examples 8-72
Part A

A reagent (1.1 eq) from Table 1 below was added to a test tube containing a solution of 1-(4-aminobutyl)-2-(2-methoxyethyl)-1H-imidazo[4,5-c]quinolin-4-amine (73 mg) in N,N-dimethylacetamide (1 mL) containing N,N-diisopropylethylamine (2 eq). The test tube was placed on a shaker overnight. The solvent was removed by vacuum centrifugation. The reaction mixtures were separated by solid-supported liquid-liquid extraction according to the following procedure. Each sample was dissolved in chloroform (1 mL) then loaded onto diatomaceous earth that had been equilibrated with de-ionized water (600 μL) for about 20 minutes. After 10 minutes chloroform (500 μL) was added to elute the product from the diatomaceous earth into a well of a collection plate. After an additional 10 minutes the process was repeated with additional chloroform (500 μL). The solvent was then removed by vacuum centrifugation.

Part B

The residue (in a test tube) was combined with dichloromethane (1 mL) and the mixture was sonicated to dissolve the solids. The solution was cooled (0° C.) and then combined with boron tribromide (400 μL of 1 M in heptane). The mixture was shaken for 5 minutes, placed in an ice bath for 30 minutes, and then shaken overnight. The solvents were removed by vacuum centrifugation. The residue was diluted with methanol (1 mL) and hydrochloric acid (500 μL of 6 N). The mixture was shaken for 30 minutes and then the solvents were removed by vacuum centrifugation. The compounds were purified by preparative high performance liquid chromatography (prep HPLC) using a Waters FractionLynx automated purification system. The prep HPLC fractions were analyzed using a Waters LC/TOF-MS, and the appropriate fractions were centrifuge evaporated to provide the trifluoroacetate salt of the desired compound. Reversed phase preparative liquid chromatography was performed with non-linear gradient elution from 5-95% B where A is 0.05% trifluoroacetic acid/water and B is 0.05% trifluoroacetic acid/acetonitrile. Fractions were collected by mass-selective triggering. Table 1 below shows the reagent used for each example, the structure of the resulting compound, and the observed accurate mass for the isolated trifluoroacetate salt.

TABLE 1

Measured

Mass

Example
Reagent
R
(M + H)

8
None

300.1840

9
Cyclopropanecarbonyl chloride

368.2063

10
Isobutyryl chloride

370.2224

11
Pivaloyl chloride

384.2390

12
Benzoyl chloride

404.2103

13
Phenyl chloroformate

420.2056

14
3-Cyanobenzoyl chloride

429.2031

15
Hydrocinnamoyl chloride

432.2377

16
Isonicotinoyl chloridehydrochloride

405.2071

17
Nicotinoyl chloridehydrochloride

405.2058

18
Methanesulfonyl chloride

378.1592

19
Ethanesulfonyl chloride

392.1729

20
1-Propanesulfonyl chloride

406.1899

21
Isopropylsulfonyl chloride

406.1888

22
Dimethylsulfamoyl chloride

407.1853

23
1 -Butanesulfonyl chloride

420.2050

24
Benzenesulfonyl chloride

440.1741

25
1-Methylimidazole-4-sulfonylchloride

444.1806

26
3-Methylbenzenesulfonylchloride

454.1895

27
alpha-Toluenesulfonyl chloride

454.1923

28
o-Toluenesulfonyl chloride

454.1944

29
p-Toluenesulfonyl chloride

454.1907

30
2-Fluorobenzenesulfonyl chloride

458.1664

31
3-Fluorobenzenesulfonyl chloride

458.1652

32
4-Fluorobenzenesulfonyl chloride

458.1639

33
3-Cyanobenzenesulfonyl chloride

465.1678

34
4-Cyanobenzenesulfonyl chloride

465.1668

35
beta-Styrene sulfonyl chloride

466.1895

36
2,5-Dimethylbenzenesulfonylchloride

468.2063

37
3,5-Dimethylbenzenesulfonylchloride

468.2046

38
2-Chlorobenzenesulfonylchloride

474.1351

39
3-Chlorobenzenesulfonylchloride

474.1385

40
4-Chlorobenzenesulfonylchloride

474.1390

41
1-Naphthalenesulfonyl chloride

490.1891

42
2-Naphthalenesulfonyl chloride

490.1885

43
2-(Trifluoromethyl)benzenesulfonylchloride

508.1592

44
3-(Trifluoromethyl)benzenesulfonylchloride

508.1612

45
4-(Trifluoromethyl)benzenesulfonylchloride

508.1640

46
2,3-Dichlorobenzenesulfonylchloride

508.0967

47
2,4-Dichlorobenzenesulfonylchloride

508.0979

48
2,5-Dichlorobenzenesulfonylchloride

508.0987

49
2,6-Dichlorobenzenesulfonylchloride

508.0968

50
3,4-Dichlorobenzenesulfonylchloride

508.0961

51
3,5-Dichlorobenzenesulfonylchloride

508.0985

52
Methyl isocyanate

357.2073

53
Ethyl isocyanate

371.2203

54
Isopropyl isocyanate

385.2347

55
n-Propyl isocyanate

385.2349

56
n-Butyl isocyanate

399.2494

57
sec-Butyl isocyanate

399.2517

58
Cyclopentyl isocyanate

411.2516

59
Cyclopropylmethylisothiocyanate

413.2133

60
Phenyl isocyanate

419.2226

61
Cyclohexyl isocyanate

425.2701

62
Benzyl isocyanate

433.2374

63
m-Tolyl isocyanate

433.2344

64
Benzoyl isocyanate

447.2126

65
2-Phenyl ethylisocyanate

447.2512

66
4-Chlorophenyl isocyanate

453.1797

67
trans-2-Phenylcyclopropylisocyanate

459.2518

68
N,N-Dimethylcarbamoyl chloride

371.2185

69
1-Pyrrolidinecarbonyl chloride

397.2382

70
1-Piperidinecarbonyl chloride

411.2526

71
4-Morpholinylcarbonyl chloride

413.2330

72
N-Methyl-N-phenylcarbamoylchloride

433.2364

Examples 73-110
Part A

Tert-Butyl 3-[4-amino-2-(2-methoxyethyl)-1H-imidazo[4,5-c]quinolin-1-yl]propylcarbamate (5 g, U.S. Pat. No. 6,573,273, example 148) and hydrochloric acid in dioxane (100 mL of 4 M) were combined and stirred for 4 hours at ambient temperature. The reaction mixture was concentrated under reduced pressure. The residue was dissolved in methanol (30 mL). The pH was adjusted to pH 8 with 6 M sodium hydroxide. The solution was diluted with dichloromethane, ethyl acetate, triethylamine, and brine. The organic layer was concentrated under reduced pressure to provide an orange solid. This material was purified by prep HPLC (COMBIFLASH system eluting first with a gradient of 0 to 10% methanol in dichloromethane containing 1% ammonium hydroxide and then with a gradient of 9 to 30% methanol in dichloromethane containing 1% ammonium hydroxide) to provide 1.58 g of 1-(3-aminopropyl)-2-(2-methoxyethyl)-1H-imidazo[4,5-c]quinolin-4-amine as a yellow solid.

Part B

A reagent (1.1 eq) from Table 2 below was added to a test tube containing a solution of 1-(3-aminopropyl)-2-(2-methoxyethyl)-1H-imidazo[4,5-c]quinolin-4-amine (30 mg) in chloroform (1 mL) containing N,N-diisopropylethylamine (1.5 eq). The test tube was placed on a shaker overnight. The reaction mixtures were separated by solid-supported liquid-liquid extraction according to the following procedure. Each reaction mixture was loaded onto diatomaceous earth that had been equilibrated with de-ionized water (600 μL) for about 20 minutes. After 10 minutes chloroform (500 μL) was added to elute the product from the diatomaceous earth into a well of a collection plate. After an additional 10 minutes the process was repeated with additional chloroform (500 μL). The solvent was then removed by vacuum centrifugation.

Part C

The ether was cleaved and the resulting product was purified using the method of Part B in Examples 8-72. Table 2 below shows the reagent used for each example, the structure of the resulting compound, and the observed accurate mass for the isolated trifluoroacetate salt.

TABLE 2

Measured Mass

Example
Reagent
R
(M + H)

73
None

286.1689

74
Propionyl chloride

342.1956

75
Cyclopropanecarbonylchloride

354.1946

76
Butyryl chloride

356.2122

77
Isobutyryl chloride

356.2119

78
Cyclobutanecarbonylchloride

368.2120

79
3-Chlorobenzoylchloride

424.1570

80
4-Chlorobenzoylchloride

424.1583

81
Nicotinoyl chloridehydrochloride

391.1913

82
trans-2-Phenyl-1-cyclopropanecarbonylchloride

430.2257

83
Methanesulfonylchloride

364.1479

84
Ethanesulfonylchloride

378.1639

85
1-Propanesulfonylchloride

392.1783

86
Isopropylsulfonylchloride

392.1788

87
Dimethylsulfamoylchloride

393.1715

88
1-Butanesulfonylchloride

406.1946

89
Benzenesulfonylchloride

426.1633

90
2,2,2-Trifluoroethanesulfonylchloride

432.1355

91
3-Methylbenzenesulfonylchloride

440.1774

92
alpha-Toluenesulfonylchloride

440.1762

93
p-Toluenesulfonylchloride

440.1790

94
3-Fluorobenzenesulfonylchloride

444.1523

95
4-Fluorobenzenesulfonylchloride

444.1545

96
3-Cyanobenzenesulfonylchloride

451.1554

97
4-Cyanobenzenesulfonylchloride

451.1582

98
Ethyl isocyanate

357.2050

99
Isopropyl isocyanate

371.2234

100
n-Butyl isocyanate

385.2364

101
Cyclopentyl isocyanate

397.2359

102
Cyclopropylmethylisothiocyanate

399.1979

103
Phenyl isocyanate

405.2040

104
Cyclohexyl isocyanate

411.2526

105
Benzyl isocyanate

419.2239

106
trans-2-Phenylcyclopropylisocyanate

445.2388

107
1-Piperidinecarbonylchloride

397.2384

108
4-Morpholinylcarbonylchloride

399.2173

109
4-Methyl-1-piperazinecarbonylchloride

412.2485

110
N-Methyl-N-phenylcarbamoylchloride

419.2229

Examples 111-140

Boron tribromide (400 μL of 1 M in heptane) was added to a tube containing a chilled (0° C.) solution of a compound of Formula Xa (about 25 mg) in dichloromethane (1 mL). The tube was vortexed, maintained at 0° C. for 0.5 hour, and then shaken overnight at ambient temperature. The reaction mixture was diluted with methanol (1 mL) and hydrochloric acid (250 μL of 6 N), vortexed, and then the solvents were removed by vacuum centrifugation. The compounds were purified by prep HPLC as described in Examples 8-72. Table 3 shows the structure of the starting material, a reference for the starting material, the structure of the resulting compound, and the observed accurate mass for the isolated trifluoroacetate salt.

TABLE 3

Reference

Measured

Example
Formula Xa
R₁
Mass (M + H)

111
U.S. Pat. No.6,756,382Example 57

455.2222

112
U.S. Pat. No.6,331,539Example 121

458.1657

113
U.S. Pat. No.6,331,539Example 111

378.1599

114
Example 3Part C

300.1853

115
U.S. Pat. No.6,541,485Example 121

413.2301

116
U.S. Pat. No.6,756,382Example 182

455.2198

117
U.S. Pat. No.6,756,382Example 183

456.2161

118
U.S. Pat. No.6,573,273Example 145

475.2829

119
U.S. Pat. No.6,677,349Example 243

434.2253

120
Example 73Part A

286.1683

121
U.S. Pat. No.6,756,382Example 187

460.2737

122
U.S. Pat. No.6,677,349Example 247

364.1446

123
U.S. Pat. No.6,573,273Example 158

411.2505

124
U.S. Pat. No.6,756,382Example 190

418.2275

125
U.S. Pat. No.6,664,264Example 16

377.1655

126
U.S. Pat. No.6,573,273Example 162

385.2358

127
U.S. Pat. No.6,677,349Example 253

440.1720

128
U.S. Pat. No.6.573,273Example 163

399.2145

129
U.S. Pat. No.6,677,349^#

314.1980

130
U.S. Pat. No.6,573,273Example 169

433.2321

131
U.S. Pat. No.6,677,349Example 256

392.1757

132
U.S. Pat. No.6,756,382Example 196

390.1929

133
U.S. Pat. No.6,683,088Example 3

408.1714

134
U.S. Pat. No.6,664,265Example 8

434.2197

135
U.S. Pat. No.6,664,265Example 73

440.2672

136
U.S. Pat. No.6,677,349^#

350.1316

137
U.S. Pat. No.6,573,273^#

343.1884

138
U.S. Pat. No.6,451,810^#

356.2078

139
U.S. Pat. No.6,677,349^#

378.1595

140
U.S. PatentPublication2004/0091491IRM3

554.4064

^#Although not specifically exemplified the compound can be readily prepared using the disclosed synthetic routes.

Example 141
N-{3-[4-Amino-2-(2-hydroxyethyl)-1H-imidazo[4,5-c]quinolin-1-yl]propyl}-2-methylpropionamide

Part A

1-(3-Aminopropyl)-2-(2-methoxyethyl)-1H-imidazo[4,5-c]quinolin-4-amine dihydrochloride (6 g, 16 mmol) was combined with triethylamine (11.2 mL, 80 mmol) and pyridine (100 mL). Isobutyryl chloride (1.9 g, 18 mmol) was added dropwise and the reaction mixture was stirred at ambient temperature for 1 hour. The reaction mixture was combined with saturated aqueous sodium bicarbonate and extracted with dichloromethane (3×200 mL). The combined organics were dried over magnesium sulfate, filtered through a layer of CELITE filter aid, and then concentrated under reduced pressure to provide 6.2 g of crude N-{3-[4-amino-2-(2-methoxyethyl)-1H-imidazo[4,5-c]quinolin-1-yl]propyl}-2-methylpropionamide as a brown solid.

Part B

The material from Part A was combined with dichloromethane (40 mL), stirred until homogeneous, and then chilled in an ice bath. Boron tribromide (40 mL of 1 M in dichloromethane) was slowly added. The ice bath was removed and the reaction mixture was stirred overnight at ambient temperature. The reaction mixture was concentrated under reduced pressure. The residue was combined with methanol (50 mL) and hydrochloric acid (50 mL of 6 N) and heated at 50° C. for 2 hours. The solution was adjusted to pH 9 with sodium hydroxide (6 M) and then extracted first with ethyl acetate (3×100 mL) and then with dichloromethane. The organics were dried over magnesium sulfate, filtered through a layer of CELITE filter aid, and then concentrated under reduced pressure. The residue was purified by prep HPLC (HORIZON HPFC system, eluting with a gradient of 0-10% methanol in dichloromethane), recrystallized from acetonitrile, and then dried in a vacuum oven to provide 208 mg of N-{3-[4-amino-2-(2-hydroxyethyl)-1H-imidazo[4,5-c]quinolin-1-yl]propyl}-2-methylpropionamide as an off-white solid, mp 196-198° C. Anal. calcd for C₁₉H₂₅N₅O₂: % C, 64.20; % H, 7.09; % N, 19.70. Found: % C, 63.99; % H, 7.28; % N, 19.63.

Example 142
1-[2-(4-Amino-2-hydroxymethyl-1H-imidazo[4,5-c]quinolin-1-yl)-1,1-dimethylethyl]-3-(1-methylethyl)urea

Part A

Under a nitrogen atmosphere, a solution of 1,2-diamino-2-methylpropane (52.20 mL, 503.3 mmol), triethylamine (131.8 mL, 958.8 mmol), and dichloromethane (1.0 L) was chilled in an ice water bath. 4-Chloro-3-nitroquinoline (100.0 g, 479.4 mmol) was added in portions over a period of 5 minutes. The reaction mixture was stirred at 0° C. for 2 hours and then allowed to slowly warm to ambient temperature. After 16 hours the reaction mixture was concentrated under reduced pressure. The residue was triturated with water (500 mL) for 1 hour. The resulting solid was isolated by filtration and dried overnight in a vacuum desiccator to provide 124.6 g of N′-(3-nitroquinolin-1-yl)-2-methylpropane-1,2-diamine as a yellow crystalline solid.

Part B

Under a nitrogen atmosphere, a suspension of N′-(3-nitroquinolin-1-yl)-2-methylpropane-1,2-diamine (60.0 g, 231 mmol) in dichloromethane (1.0 L) was chilled in an ice bath. Isopropyl isocyanate (23.8 mL, 242 mmol) was added dropwise over a period of 10 minutes. The reaction was allowed to slowly warm to room temperature. After 17 hours additional isopropyl isocyanate (about 2 mL) was added. After an additional 3 hours more isopropyl isocyanate (1 mL) was added. After 2 more hours the reaction mixture was concentrated under reduced pressure to provide 79.8 g of 1-{1,1-dimethyl-2-[(3-nitroquinolin-1-yl)amino]ethyl}-3-(1-methylethyl)urea as a bright yellow solid.

Part C

A pressure vessel was charged with the material from Part B, 5% Pt/C (4.24 g), and acetonitrile (1.5 L). The mixture was placed under hydrogen pressure for 20 hours and then filtered through a layer of CELITE filter aid. The filter cake was rinsed with additional acetonitrile. The filtrate was concentrated under reduced pressure. The residue was dissolved in toluene (750 mL) and then concentrated under reduced pressure to remove residual water. The toluene concentration was repeated. The residue was dissolved in dichloromethane (about 1 L), concentrated under reduced pressure, and then dried under high vacuum to provide 66.4 g of 1-{1,1-dimethyl-2-[(3-aminoquinolin-1-yl)amino]ethyl}-3-(1-methylethyl)urea as an orange foam.

Part D

Under a nitrogen atmosphere, a solution of 1-{1,1-dimethyl-2-[(3-aminoquinolin-1-yl)amino]ethyl}-3-(1-methylethyl)urea (66.0 g, 209 mmol) and triethylamine (32.1 mL, 230 mmol) in dichloromethane (1.0 L) was chilled in an ice bath. Ethoxyacetyl chloride (23.6 mL, 291 mmol) was added dropwise over a period of 10 minutes. The reaction mixture was allowed to slowly warm to ambient temperature overnight. The reaction mixture was concentrated under reduced pressure. The residue was combined with 1-butanol (800 mL) and triethylamine (87 mL, 627 mmol) and heated at 140° C. for 3 hours. The reaction mixture was cooled to ambient temperature and then concentrated under reduced pressure to provide a light brown foam. This material was purified by column chromatography (silica gel, eluting with 98/2/0.5 chloroform/methanol/ammonium hydroxide) to provide 29.36 g of 1-[2-(2-ethoxymethyl-1H-imidazo[4,5-c]quinolin-1-yl)-1,1-dimethylethyl]-3-(1-methylethyl)urea as a light yellow foam.

Part E

3-Chloroperoxybenzoic acid (26.33 g of 60%, 91.56 mmol) was added in portions over a period of 5 minutes to a chilled solution of the material from Part D in chloroform (350 mL). The reaction mixture was allowed to slowly warm to ambient temperature. After 2 hours the reaction mixture was chilled in an ice bath and ammonium hydroxide (100 mL) was added with vigorous stirring to homogenize. Para-toluenesulfonyl chloride (15.27 g, 80.12 mmol) was added in portions over a period of 10 minutes. The ice bath was removed and the reaction mixture was stirred for 30 minutes. The reaction mixture was diluted with water (100 mL) and chloroform (250 mL). The layers were separated. The organic layer was washed with 10% sodium carbonate (200 mL) and water (200 mL). The combined aqueous was back extracted with chloroform (100 mL). The combined organics were washed with brine (200 mL), dried over magnesium sulfate, filtered, and then concentrated under reduced pressure to provide a light brown foam. The foam was purified by column chromatography (silica gel, eluting with 95/5 chloroform/methanol) and then recrystallized from acetonitrile to provide 3.75 g of 1-[2-(4-amino-2-ethoxymethyl-1H-imidazo[4,5-c]quinolin-1-yl)-1,1-dimethylethyl]-3-(1-methylethyl)urea as an off white solid.

Part F

Under a nitrogen atmosphere, a suspension of 1-[2-(4-amino-2-ethoxymethyl-1H-imidazo[4,5-c]quinolin-1-yl)-1,1-dimethylethyl]-3-(1-methylethyl)urea (1.19 g, 2.99 mmol) in dichloromethane (30 mL) was chilled in an ice bath. Boron tribromide (7.47 mL of 1 M in dichloromethane) was added. The reaction mixture was allowed to warm slowly to ambient temperature and then stirred for 18 hours. Additional boron tribromide (2 eq) was added. After 2 hours the reaction mixture was diluted with acetonitrile (10 mL) and the reaction mixture was stirred overnight. The reaction mixture was diluted with dichloromethane (10 mL) and acetonitrile (10 mL), stirred for an additional 16 hours, quenched with methanol (25 mL), and then concentrated under reduced pressure to provide an orange foam. The foam was dissolved in hydrochloric acid (25 mL of 6 N) and heated at 50° C. for 2 hours. The solution was neutralized with 50% sodium hydroxide. The resulting gummy precipitate was extracted with chloroform (3×15 mL). The combined organics were washed with brine (15 mL), dried over magnesium sulfate, filtered, and then concentrated under reduced pressure to provide an off white solid. This material was purified by prep HPLC (HORIZON HPFC system, eluting with a gradient of 15-50% CMA in chloroform) and then recrystallized from acetonitrile to provide 335 g of 1-[2-(4-amino-2-hydroxymethyl-1H-imidazo[4,5-c]quinolin-1-yl)-1,1-dimethylethyl]-3-(1-methylethyl)urea as a white crystalline solid, mp 196-199° C.; ¹H NMR (300 MHz, DMSO-d₆) δ 8.38 (d, J=8.0 Hz, 1H), 7.59 (d, J=7.5 Hz, 1H), 7.43-7.38 (m, 1H), 7.24-7.19 (m, 1H), 6.54 (s, 2H), 5.72 (s, 1H), 5.63 (d, J=7.6 Hz, 1H), 5.46 (t, J=5.7 Hz, 1H), 5.01 (s, 2H), 4.78 (s, 2H), 3.78-3.67 (m, 1H), 1.17 (bs, 6H), 1.05 (d, J=6.9 Hz, 6H); ¹³C NMR (75 MHz, DMSO-d₆) δ 157.2, 154.2, 152.3, 145.6, 134.3, 126.8, 126.7, 121.5, 120.9, 115.8, 56.5, 54.2, 52.1, 26.4, 23.6; MS (APCI) m/z 371 (M+H)⁺; Anal. Calcd for C₁₉H₂₆N₆O₂.0.3H₂O: % C, 60.72; % H, 7.13; % N, 22.36. Found: % C, 60.44; % H, 7.42; % N, 22.52.

Example 143
N-[2-(4-Amino-2-hydroxymethyl-1H-imidazo[4,5-c][1,5]naphthyridin-1-yl)-1,1-dimethylethyl]cyclohexanecarboxamide

Part A

1,2-Diamino-2-methylpropane (8.4 mL, 80.0 mmol) was added to a chilled (0° C.) solution of 4-chloro-3-nitro[1,5]naphthyridine (15.2 g, 72.7 mmol) and triethylamine (20.2 mL, 145 mmol) in dichloromethane (350 mL). The reaction mixture was stirred overnight and then concentrated under reduced pressure. The residue was combined with water (300 mL) and heated at reflux with stirring for 1 hour. The reaction mixture was cooled and filtered. The isolated solid was washed with water and then dried under high vacuum to provide 18.5 g of N¹-(3-nitro[1,5]naphthyridin-4-yl)-2-methylpropane-1,2-diamine as a bright yellow powder.

Part B

Under a nitrogen atmosphere, a solution of sodium hydroxide (3.12 g, 78.0 mmol) in water (50 mL) was added to a solution of the material from Part A (18.5 g, 70.9 mmol) in tetrahydrofuran (200 mL). A solution of di-tert-butyl dicarbonate (17.0 g, 78.0 mmol) in tetrahydrofuran (100 mL) was added dropwise over a period of 30 minutes. Two (2) days later additional di-tert-butyl dicarbonate (2.0 g) was added. The reaction mixture was stirred for another 8 hours and then concentrated under reduced pressure. The residue was dissolved in ethyl acetate (250 mL), washed sequentially with water (×2) and brine, dried over sodium sulfate, filtered, and then concentrated under reduced pressure. The residue was dissolved in warm 1/1 ethyl acetate/hexanes. The solution was allowed to slowly cool. The resulting precipitate was isolated by filtration and washed with hexanes to provide 17.7 g of tert-butyl N-{2-[(3-nitro[1,5]naphthyridin-4-yl)amino]-1,1-dimethylethyl}carbamate as a bright yellow crystalline solid.

Part C

A Parr vessel was charged with a solution of tert-butyl N-{2-[(3-nitro[1,5]naphthyridin-4-yl)amino]-1,1-dimethylethyl}carbamate (12.62 g, 34.9 mmol) in acetonitrile (100 mL) and 5% Pt/C (2.00 g). The vessel was placed under hydrogen pressure (50 psi, 3.4×10⁵Pa) until hydrogen uptake ceased. The reaction mixture was filtered through a layer of CELITE filter aid and the filter cake was rinsed with acetonitrile. The filtrate was concentrated under reduced pressure to provide 11.07 g of tert-butyl N-{2-[(3-amino[1,5]naphthyridin-4-yl)amino]-1,1-dimethylethyl]carbamate as a bright yellow foam.

Part D

Under a nitrogen atmosphere, a solution of the material from Part C (11.07 g, 33.4 mmol) in dichloromethane (330 mL) was cooled to 0° C. Triethylamine (5.11 mL, 36.7 mmol) and ethoxyacetyl chloride (3.70 mL, 36.7 mmol) were added sequentially. The reaction mixture was stirred overnight while warming to ambient temperature and then concentrated under reduced pressure. The residue was dissolved in ethanol (300 mL). Triethylamine (16 mL) was added and the solution was heated at reflux under a nitrogen atmosphere over the weekend. The reaction mixture was allowed to cool to ambient temperature and then concentrated under reduced pressure. The residue was dissolved in dichloromethane (250 mL), washed sequentially with water and brine, dried over magnesium sulfate, filtered, and then concentrated under reduced pressure. The residue was purified by flash chromatography (6×12 cm silica gel column eluting with ethyl acetate) to provide 11.5 g of a purple foam. This material was purified by flash chromatography (eluting with 2.5% methanol in chloroform) to provide 10.07 g of tert-butyl N-[2-(2-ethoxymethyl-1H-imidazo[4,5-c][1,5]naphthyridin-1-yl)-1,1-dimethylethyl]carbamate a purple foam.

Part E

3-Chloroperoxybenzoic acid (7.50 g of 57-86%) was added to a solution of the material from Part D in dichloromethane (250 mL). After 2.5 hours, additional 3-chloroperoxybenzoic acid (250 mg) was added and the reaction mixture was stirred for 1.5 hours. The reaction mixture was washed sequentially with 1% sodium carbonate (4×75 mL), water, and brine, dried over sodium sulfate, filtered, and then concentrated under reduced pressure to provide 10.32 g of tert-butyl N-[2-(2-ethoxymethyl-5N-oxide-1H-imidazo[4,5-c][1,5]naphthyridin-1-yl)-1,1-dimethylethyl]carbamate as a purple foam.

Part F

Concentrated ammonium hydroxide (20 mL) was added to a solution of the material from Part E (10.32 g, 24.9 mmol) in dichloromethane (200 mL). Toluenesulfonyl chloride (5.02 g, 26.3 mmol) was added in small portions over a period of 2 minutes. The reaction mixture was stirred for 2 hours and then diluted with water. The layers were separated. The organic layer was washed sequentially with 1% sodium carbonate (×3), water, and brine, dried over sodium sulfate, filtered, and then concentrated under reduced pressure. The residue was purified by flash chromatography (6×15 cm column of silica gel, eluting with 10% CMA in chloroform) to provide about 8 g of a purple foam. The foam was dissolved in ethanol, combined with activated charcoal (2 g), heated at reflux for 15 minutes, filtered, and then concentrated under reduced pressure to provide 7.59 g of tert-butyl N-[2-(4-amino-2-ethoxymethyl-1H-imidazo[4,5-c][1,5]naphthyridin-1-yl)-1,1-dimethylethyl]carbamate as a violet foam.

Part G

A solution of hydrochloric acid in ethanol (17 mL of 4.3 M) was added to a solution of the material from Part F in ethanol (100 mL). The reaction mixture was heated at 90° C. for 2 hours, allowed to cool, and then concentrated under reduced pressure. The residue was dissolved in water (100 mL) and extracted with chloroform (2×25 mL). The extracts were discarded. The aqueous was made basic with concentrated ammonium hydroxide and then extracted with chloroform (4×50 mL). The combined extracts were dried over sodium sulfate, filtered, and then concentrated under reduced pressure. The residue was crystallized from ethyl acetate/hexanes (about 100 mL). The solid was isolated by filtration, rinsed with cold 20% ethyl acetate in hexanes, and dried under vacuum. A second crop was obtained and combined with the first crop to provide 3.82 g of 1-(2-amino-2-methylpropyl)-2-ethoxymethyl-1H-imidazo[4,5-c][1,5]naphthyridin-4-amine as a gray crystalline solid.

Part H

Under a nitrogen atmosphere, a solution of 1-(2-amino-2-methylpropyl)-2-ethoxymethyl-1H-imidazo[4,5-c][1,5]naphthyridin-4-amine (1.552 g, 4.94 mmol) in dichloromethane (50 mL) was cooled to 0° C. Triethylamine (1.38 mL, 9.92 mmol) and cyclohexylcarbonyl chloride (661 μL, 4.94 mmol) were added sequentially. Two (2) days later the reaction mixture was cooled and additional cyclohexylcarbonyl chloride (40 μL) was added. The reaction mixture was stirred overnight and then diluted with saturated sodium bicarbonate and dichloromethane (50 mL). The layers were separated. The organic layer was washed sequentially with water (×2) and brine, dried over sodium sulfate, filtered, and then concentrated under reduced pressure. The residue was purified by flash chromatography (4×13 cm silica gel column, eluting with 3% methanol in chloroform). The purified material was dissolved in refluxing propyl acetate (80 mL) with the aid of methanol, the methanol was boiled off, and the solution was allowed to slowly cool. The resulting precipitate was isolated by filtration, rinsed with cold propyl acetate, and dried under high vacuum at 70° C. to provide 1.37 g of N-[2-(4-amino-2-ethoxymethyl-1H-imidazo[4,5-c][1,5]naphthyridin-1-yl)-1,1-dimethylethyl]cyclohexanecarboxamide as a colorless crystalline solid, mp 210-211° C. Anal. calcd for C₂₃H₃₂N₆O₂: % C, 65.07; % H, 7.60; % N, 19.80. Found: % C, 64.93; % H, 7.76; % N, 19.97.

Part I

Boron tribromide (1.24 mL of 1 M in dichloromethane) was added dropwise to a chilled (ice bath) suspension of N-[2-(4-amino-2-ethoxymethyl-1H-imidazo[4,5-c][1,5]naphthyridin-1-yl)-1,1-dimethylethyl]cyclohexanecarboxamide (500 mg, 1.18 mmol) in dichloromethane (15 mL). The reaction mixture was allowed to slowly warm to ambient temperature and then stirred over the weekend. Additional boron tribromide (1 mL) was added and the reaction mixture was stirred for 24 hours. The reaction was quenched with methanol (10 mL) and then concentrated under reduced pressure. The residue was combined with hydrochloric acid (15 mL of 6 M), heated to 50° C., and stirred for 2 hours. The resulting solution was cooled to ambient temperature and then neutralized (pH 7) with 10% sodium hydroxide. The resulting gummy precipitate was extracted with chloroform (3×15 mL). The combined extracts were washed with brine (15 mL), dried over sodium sulfate, filtered, and then concentrated under reduced pressure to provide an off white solid. This material was purified by prep HPLC (HORIZON HPFC system, eluting with a gradient of 10-50% CMA in chloroform) to provide a white solid. The solid was triturated with hot acetonitrile, allowed to cool, isolated by filtration, and dried under vacuum to provide 233 mg of N-[2-(4-amino-2-hydroxymethyl-1H-imidazo[4,5-c][1,5]naphthyridin-1-yl)-1,1-dimethylethyl]cyclohexanecarboxamide as a fine white solid, mp 230-232° C.; ¹H NMR (300 MHz, DMSO-d₆, 350 K) δ 8.53 (dd, J=4.3, 1.6 Hz, 1H), 7.95 (dd, J=8.4, 1.5 Hz, 1H), 7.87 (s, 1H), 7.47 (dd, J=8.4, 4.4 Hz, 1H), 6.55 (s, 2H), 5.31 (s, 1H), 5.15 (s, 2H), 4.79 (d, J=5.4 Hz, 2H), 1.90-1.80 (m, 1H), 1.67-1.43 (m, 5H), 1.31 (s, 6H), 1.24-1.02 (m, 5H); ¹³C NMR (75 MHz, DMSO-d₆) δ 175.9, 154.6, 152.8, 142.8, 140.8, 134.2, 133.5, 133.3, 129.3, 122.5, 56.4, 55.0, 52.3, 44.9, 29.4, 25.7, 25.6, 24.9; MS (ESI) m/z 397 (M+H)⁺; Anal, Calcd for C₂₁H₂₈N₆O₂: C, 63.62; H, 7.12; N, 21.20. Found: C, 63.77; H, 7.34; N, 21.50.

Example 144
N-[2-(4-Amino-2-hydroxymethyl-1H-imidazo[4,5-c][1,5]naphthyridin-1-yl)-1,1-dimethylethyl]methanesulfonamide

Part A

Under a nitrogen atmosphere, a solution of 1-(2-amino-2-methylpropyl)-2-ethoxymethyl-1H-imidazo[4,5-c][1,5]naphthyridin-4-amine (1.588 g, 5.06 mmol) in dichloromethane (50 mL) was cooled to 0° C. Triethylamine (1.41 mL, 10.12 mmol) and methanesulfonyl chloride (392 μL, 5.06 mmol) were added sequentially. The reaction mixture was allowed to slowly warm to ambient temperature overnight. Additional methanesulfonyl chloride (40 μL) was added and the reaction mixture was stirred at ambient temperature for an additional 5 hours. The reaction mixture was diluted with aqueous saturated sodium bicarbonate and the layers were separated. The organic layer was washed sequentially with water and brine, dried over sodium sulfate, filtered, and then concentrated under reduced pressure. The residue was purified by flash chromatography (4×15 cm silica gel column, eluting with a gradient of 5-7.5% methanol in chloroform). The purified material was dissolved in refluxing propyl acetate (80 mL) with the aid of methanol, the methanol was boiled off, and the solution was allowed to slowly cool. The resulting precipitate was isolated by filtration, rinsed with cold propyl acetate, and dried under high vacuum at 70° C. to provide 1.35 g of N-[2-(4-amino-2-ethoxoxymethyl-1H-imidazo[4,5-c][1,5]naphthyridin-1-yl)-1,1-dimethylethyl]methanesulfonamide as colorless needles, mp 209-210° C. Anal. calcd for C₁₇H₂₄N₆O₃S: % C, 52.02; % H, 6.16; % N, 21.41. Found: % C, 52.09; % H, 6.35; % N, 21.60.

Part B

Boron tribromide (1.34 mL of 1 M in dichloromethane) was added dropwise to a chilled (ice bath) suspension of N-[2-(4-amino-2-ethoxoxymethyl-1H-imidazo[4,5-c][1,5]naphthyridin-1-yl)-1,1-dimethylethyl]methanesulfonamide (500 mg, 1.27 mmol) in dichloromethane (15 mL). The reaction mixture was allowed to slowly warm to ambient temperature and then stirred over the weekend. Additional boron tribromide (1.5 mL) was added and the reaction mixture was stirred for 4 hours. Additional boron tribromide (1.5 mL) was added and the reaction mixture was stirred overnight. The reaction was quenched with methanol (15 mL) and then concentrated under reduced pressure. The residue was combined with hydrochloric acid (15 mL of 6 M), heated to 50° C., and stirred for 2 hours. The resulting solution was cooled to ambient temperature and then neutralized (pH 7) with 10% sodium hydroxide. The resulting precipitate was isolated by filtration and rinsed with water to provide a white solid. This material was purified by prep HPLC (HORIZON HPFC system, eluting with a gradient of 10-50% CMA in chloroform) to provide a white solid. This material was recrystallized from acetonitrile and dried in a vacuum oven to provide 103 mg of N-[2-(4-amino-2-hydroxymethyl-1H-imidazo[4,5-c][1,5]naphthyridin-1-yl)-1,1-dimethylethyl]methanesulfonamide as a white crystalline solid, mp 268-271° C.; ¹H NMR (300 MHz, DMSO-d₆) δ 8.50 (dd, J=4.4, 1.6 Hz, 1H), 7.95 (dd, J=8.4, 1.5 Hz, 1H), 7.90 (s, 1H), 7.48 (dd, J=8.4, 4.4 Hz, 1H), 6.91 (s, 2H), 5.62 (t, J=5.9 Hz, 1H), 5.10 (bs, 2H), 4.92 (s, 2H), 2.87 (s, 3H), 1.35 (s, 6H); ¹³C NMR (75 MHz, DMSO-d₆) δ 154.2, 152.3, 142.3, 140.3, 133.4, 133.1, 132.9, 128.8, 122.1, 57.2, 56.4, 54.3, 44.1, 25.1; MS (APCI) m/z 365 (M+H)⁺; Anal. Calcd for C₁₅H₂₀N₆O₃S: C, 49.44; H, 5.53; N, 23.06. Found: C, 49.48; H, 5.40; N, 23.31.

Example 145
N-{4-{4-Amino-2-(2-hydroxyethyl)-1H-imidazo[4,5-c][1,5]naphthyridin-1-yl]butyl}methanesulfonamide

Part A

3-Methoxypropionyl chloride (2.7 g, 22 mmol) was added dropwise to a chilled (ice bath) solution of tert-butyl N-{4-[(3-amino[1,5]naphthyridin-4-yl)amino]butyl}carbamate 6.7 g, 20 mmol, U.S. Pat. No. 6,194,425, Example 42) in anhydrous pyridine (75 mL). The reaction mixture was heated at 120° C. overnight. The reaction was repeated on the same scale. The reaction mixtures were combined and concentrated under reduced pressure to provide 28 g of crude tert-butyl N-((4-{([3-(3-methoxypropionyl)amino[1,5]naphthyridin-4-yl]amino}butyl))carbamate as a red oil.

Part B

The crude material from Part A was dissolved in pyridine (150 mL). Pyridine hydrochloride (2.1 g) was added and the reaction mixture was heated at reflux overnight. The reaction mixture was concentrated under reduced pressure. The residue was diluted with dichloromethane and washed with brine. The aqueous layer was extracted with dichloromethane (×4). The combined organics were concentrated under reduced pressure. The residue was purified by prep HPLC (COMBIFLASH system eluting with a gradient of 0-7% methanol in dichloromethane containing 1% ammonium hydroxide) to provide 9.72 g of tert-butyl N-{4-[2-(2-methoxyethyl)-1H-imidazo[4,5-c][1,5]naphthyridin-1-yl]butyl}carbamate as a brown glassy solid.

Part C

3-Chloroperoxybenzoic acid (7.8 g of 77%) was added in a single portion to a solution of tert-butyl N-{4-[2-(2-methoxyethyl)-1H-imidazo[4,5-c][1,5]naphthyridin-1-yl]butyl}carbamate (7 g) in dichloroethane (100 mL). The reaction mixture was stirred at ambient temperature for 3 hours, Concentrated ammonium hydroxide (100 mL) was added and the reaction mixture was stirred until a suspension formed. Para-toluenesulfonyl chloride (3.6 g) was added in a single portion. The reaction mixture was stirred at ambient temperature for 2 hours and then diluted with dichloromethane and brine. The organic layer was separated, washed with brine (×2), dried over magnesium sulfate, filtered through a layer of CELITE filter aid, and then concentrated under reduced pressure to provide 8.83 g of crude tert-butyl N-{4-[4-amino-2-(2-methoxyethyl)-1H-imidazo[4,5-c][1,5]naphthyridin-1-yl]butyl}carbamate as a brown solid.

Part D

The material from Part C was diluted with a small amount of dichloromethane and then hydrochloric acid in dioxane (126 mL of 4 M) was slowly added. The reaction mixture was stirred at ambient temperature overnight and then concentrated under reduced pressure. The residue was purified by prep HPLC (COMBIFLASH system eluting with a gradient of 0-7% methanol in dichloromethane containing 1% ammonium hydroxide) to provide 8 g of crude 1-(4-aminobutyl)-2-(2-methoxyethyl)-1H-imidazo[4,5-c][1,5]naphthyridin-4-amine.

Part E

Triethylamine (3.9 mL) was added to a solution of a portion (1.8 g) of the material from Part D in pyridine (20 mL). Methanesulfonyl chloride (485 μL) was added dropwise. The reaction mixture was stirred at ambient temperature for 2 hours, quenched with water (25 mL), and the stirred overnight. The reaction mixture was concentrated under reduced pressure and then diluted with dichloromethane. The organic layer was washed with brine (×2) and then concentrated under reduced pressure. The residue was purified by prep HPLC (COMBIFLASH system eluting with a gradient of 0-5% methanol in dichloromethane containing 1% ammonium hydroxide for 5 minutes and then holding at 5%) to provide 400 mg of N-{4-{4-amino-2-(2-methoxyethyl)-11H-imidazo[4,5-c][1,5]naphthyridin-1-yl]butyl}methanesulfonamide.

Part F

Boron tribromide (2.55 mL of 1 M in dichloromethane) was slowly added to a chilled mixture of the material from Part E in dichloromethane (10 mL). The reaction mixture was stirred at ambient temperature overnight and then concentrated under reduced pressure. The residue was dissolved in methanol, combined with hydrochloric acid (50 mL of 6 M), heated at 50° C. for 2 hours, and concentrated under reduced pressure. The residue was combined with a solution of ammonia in methanol (about 50 mL of 7 M) and then concentrated again. This procedure was repeated 3 times. The residue from the final concentration was purified by prep HPLC (COMBIFLASH system eluting with a gradient of 0-10% methanol in dichloromethane containing 1% ammonium hydroxide for 10 minutes). The combined fractions were concentrated and then distributed onto solid phase extraction cartridges. The cartridges were eluted with ammonia in methanol (7 M). The resulting material was triturated with hot acetonitrile, cooled, isolated, and then dried in a vacuum oven to provide 111 mg of N-{4-{4-amino-2-(2-hydroxyethyl)-1H-imidazo[4,5-c][1,5]naphthyridin-1-yl]butyl}methanesulfonamide, nip 194-195° C. Anal. calcd for C₁₆H₂₂N₆O₃S: % C, 50.78; % H, 5.86; % N, 22.21. Found: % C, 50.83; % H, 6.12; % N, 21.70.

Example 146
N-[2-(4-Amino-2-hydroxymethyl-1H-imidazo[4,5-c][1,5]naphthyridin-1-yl)ethyl]methanesulfonamide

Part A

Methoxyacetyl chloride (5.9 g, 54 mmol) was added dropwise to a chilled (ice bath) solution of tert-butyl N-{2-[(3-amino[1,5]naphthyridin-4-yl)amino]ethyl}carbamate (15.0 g, 49.5 mmol, U.S. Pat. No. 6,194,425, Example 87) in anhydrous pyridine (100 mL). The reaction mixture was heated at reflux until analysis by liquid chromatography/mass spectroscopy (LCMS) indicated that the reaction was complete. The reaction mixture was concentrated under reduced pressure. The residue was diluted with ethanol (100 mL), combined with potassium carbonate solution (200 mL of 2 M), and heated at reflux for 4 hours. The reaction mixture was cooled and then concentrated under reduced pressure. The residue was partitioned between water and dichloromethane. The aqueous layer was extracted with dichloromethane. The combined organics were dried over magnesium sulfate, filtered, and then concentrated under reduced pressure to provide 14 g of tert-butyl N-[2-(2-methoxymethyl-1H-imidazo[4,5-c][1,5]naphthyridin-1-yl)ethyl]carbamate.

Part B

Using the method of Example 145 Part C, the material from Part A was oxidized and then aminated to provide 17 g of crude tert-butyl N-[2-(4-amino-2-methoxymethyl-1H-imidazo[4,5-c][1,5]naphthyridin-1-yl)ethyl]carbamate as a sticky amber solid.

Part C

The material from Part B was dissolved in a mixture of dichloromethane (20 mL) and methanol (5 mL). Hydrochloric acid in dioxane (28 mL of 4 M) was added. More dichloromethane was added to facilitate stirring. The reaction mixture was stirred at ambient temperature overnight and then concentrated under reduced pressure to provide crude 1-(2-aminoethyl)-2-methoxymethyl)-1H-imidazo[4,5-c][1,5]naphthyridin-4-amine as an orange solid.

Part D

Triethylamine (35.6 mL) was added to a mixture of the material from Part C and pyridine (100 mL). The reaction mixture was cooled in an ice bath and then methanesulfonyl chloride (4.3 mL) was added dropwise. The reaction mixture was stirred at ambient temperature for 1 hour. Twice, more methanesulfonyl chloride (0.43 mL) was added and the reaction mixture was stirred at ambient temperature for 2 hours. The reaction mixture was partitioned between water and dichloromethane. The aqueous layer was extracted with dichloromethane (×2). The combined organics were dried over magnesium sulfate, filtered through a layer of CELITE filter aid, and then concentrated under reduced pressure to provide 14 g of N-[2-(4-amino-2-methoxymethyl-1H-imidazo[4,5-c][1,5]naphthyridin-1-yl)ethyl]methanesulfonamide.

Part E

Boron tribromide (71.4 mL of 1 M in dichloromethane) was slowly added to a chilled (ice bath) mixture of the material from Part D in dichloromethane (50 mL). The reaction mixture was stirred at ambient temperature for 2 hours. Additional boron tribromide (0.5 eq) was added and the reaction mixture was stirred at ambient temperature overnight. The reaction mixture was concentrated under reduced pressure. The residue was dissolved in methanol, combined with hydrochloric acid (50 mL of 6 M), heated at 50° C. for 2 hours, and concentrated under reduced pressure. The residue was combined with a solution of ammonia in methanol (about 40 mL of 7 M) and then concentrated again. This procedure was repeated 3 times. The residue from the final concentration was purified by prep HPLC (COMBIFLASH system eluting with a gradient of 0-5% methanol in dichloromethane containing 1% ammonium hydroxide with a 10 minute ramp and a 20 minute hold, then with gradient of 6-10% methanol in dichloromethane containing 1% ammonium hydroxide with a 10 minute ramp and a 20 minute hold, and finally with gradient of 11-20% methanol in dichloromethane containing 1% ammonium hydroxide with a 10 minute ramp and a 20 minute hold) to provide 2.4 g of a brown solid. A small portion of this material was combined with hot acetonitrile containing a small amount of methanol, cooled, and then isolated by filtration. This procedure was carried out 3 times. After the final isolation the material was rinsed with ether and dried in a vacuum oven to provide 75 mg of N-[2-(4-amino-2-hydroxymethyl-1H-imidazo[4,5-c][1,5]naphthyridin-1-yl)ethyl]methanesulfonamide as a beige solid, mp 239-242° C. Anal. calcd for C₁₃H₁₆N₆O₃S: % C, 46.42; % H, 4.79; % N, 24.98. Found: % C, 46.35; % H, 4.70; % N, 24.70.

Example 147
N-{2-[4-Amino-2-(2-hydroxyethyl)-1H-imidazo[4,5-c][1,5]naphthyridin-1-yl]ethyl}methanesulfonamide

Part A

Using the general method of Example 146 Part A, tert-butyl N-{2-[(3-amino[1,5]naphthyridin-4-yl)amino]ethyl}carbamate (17.0 g, 56.1 mmol) was reacted with 3-methoxypropionyl chloride (7.5 g, 61.7 mmol) to provide 9.0 g of crude product. Analysis by LCMS indicated that the crude product was about a 1:1 mixture of tert-butyl N-{2-[2-(2-methoxyethyl)-1H-imidazo[4,5-c][1,5]naphthyridin-1-yl]ethyl}carbamate and 1-(2-aminoethyl)-2-(2-methoxyethyl)-1H-imidazo[4,5-c][1,5]naphthyridine.

Part B

Triethylamine (13.8 mL) was added to a mixture of the material from Part A and dichloromethane (70 mL). The resulting solution was chilled in an ice bath. Di-tert-butyl dicarbonate (8.6 g) was added. The reaction mixture was stirred at ambient temperature for 2 hours and then quenched with water. The layers were separated. The organic layer was washed with sodium carbonate, dried over magnesium sulfate, filtered through a layer of CELITE filter aid, and then concentrated under reduced pressure to provide 11 g of tert-butyl N-{2-[2-(2-methoxyethyl)-1H-imidazo[4,5-c][1,5]naphthyridin-1-yl]ethyl}carbamate as a tan solid.

Part C

3-Chloroperoxybenzoic acid (13.2 g of 77%) was added in a single portion to a solution of the material from Part B (11 g, 29.6 mmol) in dichloroethane (50 mL). The reaction mixture was stirred at ambient temperature for 1.5 hours, then diluted with dichloromethane and washed with aqueous ammonium hydroxide (25 mL of concentrated ammonium hydroxide in 250 mL of water). The aqueous layer was extracted with dichloromethane. The combined organics were concentrated under reduced pressure. The residue was dissolved in dichloroethane (100 mL). Concentrated ammonium hydroxide (70 mL) was added and the reaction mixture was stirred until a suspension formed. Para-Toluenesulfonyl chloride (6.2 g, 32.5 mmol) was added in a single portion. The reaction mixture was stirred at ambient temperature for 1.5 hours, then diluted with aqueous sodium bicarbonate and extracted with dichloromethane (×3). The combined organics were dried over magnesium sulfate, filtered through a layer of CELITE filter aid, and then concentrated under reduced pressure. The residue was purified by prep HPLC (COMBIFLASH system eluting with a gradient of 0-5% methanol in dichloromethane containing 1% ammonium hydroxide over 6 minutes and then holding at 5%) to provide 3.5 g of tert-butyl N-{2-[4-amino-2-(2-methoxyethyl)-1H-imidazo[4,5-c][1,5]naphthyridin-1-yl]ethyl}carbamate as an orange solid.

Part D

A solution of hydrochloric acid in dioxane (58 mL of 4 M) was added to a solution of tert-butyl N-{2-[4-amino-2-(2-methoxyethyl)-1H-imidazo[4,5-c][1,5]naphthyridin-1-yl]ethyl}carbamate (3 g) in a small amount of dichloromethane/methanol. The reaction mixture was stirred overnight at ambient temperature and then concentrated under reduced pressure to provide 3.7 g of crude 1-(2-aminoethyl)-2-(2-methoxyethyl)-1H-imidazo[4,5-c][1,5]naphthyridine-4-amine hydrochloride.

Part E

Using the general method of Example 146 Part D, a portion (1.1 g) of the material from Part D was reacted with methanesulfonyl chloride (322 μL) to provide 1.0 g of N-{2-[4-amino-2-(2-methoxyethyl)-1H-imidazo[4,5-c][1,5]naphthyridin-1-yl]ethyl}methanesulfonamide as a red solid.

Part F

Boron tribromide (7 mL of 1 M in dichloromethane) was slowly added to a chilled (ice bath) mixture of the material from Part E in dichloromethane (25 mL). The reaction mixture was stirred at ambient temperature overnight and then concentrated under reduced pressure. The residue was dissolved in methanol, combined with hydrochloric acid (50 mL of 6 M), heated at 50° C. for 2 hours, and concentrated under reduced pressure. The residue was combined with a solution of ammonia in methanol (about 30 mL of 7 M) and then concentrated again. This procedure was repeated 3 times. The residue from the final concentration was purified by prep HPLC (COMBIFLASH system eluting with a gradient of 0-10% methanol in dichloromethane containing 1% ammonium hydroxide). The residue was combined with hot acetonitrile, cooled, and the acetonitrile was decanted off. This procedure was carried out 3 times. The material was isolated by filtration, rinsed with ether and dried in a vacuum oven to provide 950 mg of N-{2-[4-amino-2-(2-hydroxyethyl)-1H-imidazo[4,5-c][1,5]naphthyridin-1-yl]ethyl}methanesulfonamide, mp 136-138° C. Anal. calcd for C₁₄H₁₈N₆O₃S: % C, 47.99; % H, 5.18; % N, 23.98. Found: % C, 47.69; % H, 5.36; % N, 23.77.

Example 148
1-(4-Amino-2-hydroxymethyl-1H-imidazo[4,5-c][1,5]naphthyridin-1-yl)-2-methylpropan-2-ol

Part A

Under a nitrogen atmosphere, 1-amino-2-methylpropan-2-ol (25.5 g, 0.28 mol) was added over a period of 30 minutes to a solution of 4-chloro-3-nitro[1,5]naphthyridine (54.5 g, 0.26 mol) in dichloromethane (1 L). A water bath was used to control the exotherm and maintain the temperature of the reaction at or below 27° C. The reaction mixture was stirred at ambient temperature overnight. The resulting precipitate (crop 1) was isolated by filtration. The filtrate was concentrated under reduced pressure to provide crop 2. The two crops were slurried separately with de-ionized water for 2 hours and then isolated by filtration. Crop 1: 40.53 g of 2-methyl-2-[(3-nitro[1,5]naphthyridin-4-yl)amino]propan-2-ol as a yellow solid. Crop 2: tan solid. Crop 2 was dissolved in dichloromethane and loaded onto an alumina column. The column was eluted first with 1% methanol in dichloromethane and then with acetone. The combined eluents were concentrated under reduced pressure. The residue was recrystallized from ethanol (10 mL/g) to provide 6.95 g of 2-methyl-1-[(3-nitro[1,5]naphthyridin-4-yl)amino]propan-2-ol.

Part B

A Parr vessel was charged with 2-methyl-1-[(3-nitro[1,5]naphthyridin-4-yl)amino]propan-2-ol (44.12 g, 0.17 mol), 5% Pt/C (4.4 g) and isopropyl alcohol (890 mL). The vessel was placed under hydrogen pressure (35 psi, 2.4×10⁵Pa) until hydrogen uptake ceased. The reaction mixture was filtered through a layer of filter aid. The filter cake was rinsed with additional isopropyl alcohol. The filtrate was concentrated under reduced pressure to provide 1-[(3-amino[1,5]naphthyridin-4-yl)amino]-2-methylpropan-2-ol as a thick oil.

Part C

Under a nitrogen atmosphere, ethoxyacetyl chloride (19.1 g, 0.156 mol) was added over a period of 12 minutes to a mixture of 1-[(3-amino[1,5]naphthyridin-4-yl)amino]-2-methylpropan-2-ol (28.95 g, 0.125 mol) in pyridine (300 mL). The reaction mixture was stirred at ambient temperature for 4 hours and then at reflux for 4 hours. The reaction mixture was allowed to cool to ambient temperature overnight and then concentrated under high vacuum. The residue was dissolved in 5% potassium carbonate (200 mL) and then extracted with dichloromethane (200 mL). The extract was filtered to remove some insoluble material, dried over magnesium sulfate, filtered, and then concentrated under high vacuum. The residue was dissolved in dichloromethane (150 mL) and eluted through a short column of alumina. The eluent was concentrated under reduced pressure and air dried to provide 31.9 g of 1-(2-ethoxymethyl-1H-imidazo[4,5-c][1,5]naphthyridin-1-yl)-2-methylpropan-2-ol.

Part D

A flask containing a solution of 1-(2-ethoxymethyl-1H-imidazo[4,5-c][1,5]naphthyridin-1-yl)-2-methylpropan-2-ol (29.94 g, 83 mmol) in dichloromethane (300 mL) was covered with aluminum foil. 3-Chloroperoxybenzoic acid (28.65 g of 50%) was added in portions over a period of 50 minutes. The reaction mixture was stirred for an additional 40 minutes, then diluted with 5% aqueous potassium carbonate and stirred. The organic layer was separated, washed with brine (100 mL), dried over magnesium sulfate, filtered, and then concentrated under reduced pressure to provide a yellow paste. This material was combined with ether (100 mL) and stirred overnight. The resulting solid was isolated by filtration to provide 11.84 g of 1-(2-ethoxymethyl-5-oxo-1H-imidazo[4,5-c][1,5]naphthyridin-1-yl)-2-methylpropan-2-ol. The aqueous potassium carbonate layer was partially concentrated, saturated with additional potassium carbonate, and then extracted with dichloromethane. The extract was dried over magnesium sulfate, filtered, and then concentrated under reduced pressure to provide 15.23 g of a dark oil. The oil was combined with ether (100 mL) and stirred overnight. The resulting solid was isolated by filtration to provide 11.51 g of 1-(2-ethoxymethyl-5-oxo-1H-imidazo[4,5-c][1,5]naphthyridin-1-yl)-2-methylpropan-2-ol.

Part E

Concentrated ammonium hydroxide (241 mL) was added to a solution of 1-(2-ethoxymethyl-5-oxo-1H-imidazo[4,5-c][1,5]naphthyridin-1-yl)-2-methylpropan-2-ol (23.35 g, 74 mmol) in dichloromethane (300 mL). A solution of para-toluenesulfonyl chloride (15.52 g, 81 mmol) in dichloromethane (50 mL) was added with rapid stirring over a period of 25 minutes. The reaction mixture was stirred overnight. Concentrated ammonium hydroxide (25 mL) and a solution of para-toluenesulfonyl chloride (2 g) in dichloromethane (10 mL) was added and the reaction mixture was stirred for 5 hours. The organic phase was separated, washed with a solution of potassium carbonate (16 g) in water (300 mL), dried over magnesium sulfate, filtered, and concentrated under reduced pressure to provide 30.17 g of crude product. This material was combined with acetonitrile (300 mL), stirred, heated to reflux, and then allowed to cool with stirring to ambient temperature. The resulting solid was isolated by filtration and then dried at 75° C. under vacuum to provide 14.4 g of a solid. This material was recrystallized from ethyl acetate (17.5 mL/g), isolated by filtration, and then dried under vacuum at 75° C. for 22 hours to provide 12.29 g of 1-(4-amino-2-ethoxymethyl-1H-imidazo[4,5-c][1,5]naphthyridin-1-yl)-2-methylpropan-2-ol as an off white solid, mp 157-159° C. Anal. calcd for C₁₆H₂₁N₅O₂: % C, 60.94; % H, 6.71; % N, 22.21. Found: % C, 61.06; % H, 6.67; % N, 22.37.

Part F

A solution of boron tribromide in dichloromethane (11.8 mL of 1 M) was added to a chilled (0° C.) suspension of 1-(4-amino-2-ethoxymethyl-1H-imidazo[4,5-c][1,5]naphthyridin-1-yl)-2-methylpropan-2-ol hydrobromide (1.24 g, 3.93 mmol) in dichloromethane (30 mL). The reaction mixture was allowed to come to ambient temperature with stirring for 16 hours. Methanol (15 mL) and hydrochloric acid (10 mL of 6 N) were added and the reaction mixture was heated at reflux for 2.5 hours. The reaction mixture was made basic with sodium hydroxide and the layers were separated. The aqueous layer was extracted with ethyl acetate (3×100 mL). The combined extracts were washed sequentially with water and brine, dried over magnesium sulfate, and then concentrated under reduced pressure to provide a white solid. This material was crystallized from ethyl acetate and then dried under vacuum at 95° C. for 16 hours to provide 0.55 g of 1-(4-amino-2-hydroxymethyl-1H-imidazo[4,5-c][1,5]naphthyridin-1-yl)-2-methylpropan-2-ol as a white powder, mp 235-237° C. Anal. calcd for C₁₄H₁₇N₅O₂: % C, 58.52; % H, 5,96; % N, 24.37. Found: % C, 58.40; % H, 5.82; % N, 24.45.

Example 149
1-[4-Amino-2-(2-hydroxyethyl)-1H-imidazo[4,5-c][1,5]naphthyridin-1-yl]-2-methylpropan-2-ol

Part A

A mixture of triethyl orthoformate (10 mL, 60.1 mmol) and 2,2-dimethyl-[1,3]-dioxane-4,6-dione (40.9 g, 0.23 mol) (Meldrum's acid) was heated at 92° C. for 90 minutes and then cooled to 70° C. over one hour. 3-Amino-5-bromopyridine (40.9 g, 0.20 mol) was slowly added over 10 minutes with an ethanol rinse while maintaining the reaction temperature between 60 and 70° C. The reaction was then heated for an additional 20 minutes and allowed to cool to room temperature. The reaction mixture was filtered and washed with ethanol (150 mL) yielding a tan solid. The solid was dried under vacuum for 2 hours to yield 59.14 g of 5-{[(5-bromopyridin-3-yl)imino]methyl}-2,2-dimethyl-1,3-dioxane-4,6-dione as a light yellow crystalline solid, mp 200-202° C.

Part B

5-{[(5-Bromopyridin-3-yl)imino]methyl}-2,2-dimethyl-1,3-dioxane-4,6-dione (59 g, 0.18 mol) was slowly added to DOWTHERM A heat transfer fluid (2000 mL) over a period of 5 minutes at 235-238° C. Following addition, the reaction was maintained for an additional 5 minutes and then allowed to cool to 40° C. A brown precipitate formed, which was filtered and washed with hexanes (150 mL). The brown solid was suspended in an ethanol/water mixture (90:10, 1500 mL), heated to a boil for 30 minutes, isolated by filtration, and washed with ethanol (200 mL) to yield 30.8 g of 7-bromo[1,5]naphthyridin-4-ol as a dark brown powder.

Part C

A mixture of 7-bromo[1,5]naphthyridin-4-ol (33 g, 0.147 mol) and fuming nitric acid (350 mL) was heated at reflux (90° C. internal reaction vessel temperature) for 3 hours. The reaction mixture was cooled to 50° C., poured over 1 L of ice and neutralized to pH 2-3 with a solution of 50% aqueous sodium hydroxide. The resulting precipitate was filtered, washed with water, and dried over vacuum for 3 days to yield 25.1 g of 7-bromo-3-nitro[1,5]naphthyridin-4-ol as a yellow crystalline solid.

Part D

Phosphorous oxychloride (16.76 g, 10.19 mL, 109.3 mmol) was added slowly dropwise to a suspension of 7-bromo-3-nitro[1,5]naphthyridin-4-ol (21.09 g, 78.1 mmol) in N,N-dimethylformamide (250 mL) (DMF) at ambient temperature and maintained overnight. The reaction mixture was then added to ice water (400 mL) with stirring. A solid precipitate formed, which was isolated by vacuum filtration and washed with water. The material was dried under high vacuum at ambient temperature overnight to yield 20.79 g of 7-bromo-4-chloro-3-nitro[1,5]naphthyridine as a tan solid.

Part E

Triethylamine (35.95 mL, 257.9 mmol) was added to a suspension of 7-bromo-4-chloro-3-nitro[1,5]naphthyridine (49.6 g, 172 mmol) in dichloromethane (500 mL). 1-Amino-2-methylpropan-2-ol (16.86 g, 189 mmol) was added dropwise. The reaction mixture was stirred at ambient temperature for 16 hours and then concentrated under reduced pressure. The residue was triturated with water and stirred for 1 hour. The precipitated solid was isolated by filtration, washed with water, and dried. This material was suspended in diethyl ether (400 mL), sonicated, isolated by filtration, and then dried in a vacuum oven at 40° C. for 16 hours to provide 58.1 g of 1-[(7-bromo-3-nitro[1,5]naphthyridin-4-yl)amino]-2-methylpropan-2-ol as a yellow solid, mp 189-190° C.

Part F

A Parr vessel was charged with 5% Pt/C (5.8 g) and a suspension of 1-[(7-bromo-3-nitro[1,5]naphthyridin-4-yl)amino]-2-methylpropan-2-ol (58.00 g) in acetonitrile (800 mL) and methanol (400 mL). The vessel was placed under hydrogen pressure (30 psi, 2.1×10⁵Pa) for 8 hours. The reaction mixture was filtered through a layer of CELITE filter aid. The filtrate was concentrated under reduced pressure to provide 52.70 g of 1-[(3-amino-7-bromo[1,5]naphthyridin-4-yl)amino]-2-methylpropan-2-ol as a yellow foam.

Part G

3-Methoxypropionyl chloride (24.90 g, 203 mmol) was added over a period of 5 minutes to a mixture of 1-[(3-amino-7-bromo[1,5]naphthyridin-4-yl)amino]-2-methylpropan-2-ol (52.70 g, 169 mmol), chloroform (100 mL), and acetonitrile (530 mL). The reaction mixture was stirred at ambient temperature overnight. The precipitated solid was isolated by filtration, washed well with acetonitrile, and then dried to provide 60.10 g of N-{7-bromo-4-[(2-hydroxy-2-methylpropyl)amino][1,5]naphthyridin-3-yl}-3-methoxypropionamide hydrochloride as a brown solid, mp 206-208° C.

Part H

A mixture of N-{7-bromo-4-[(2-hydroxy-2-methylpropyl)amino][1,5]naphthyridin-3-yl}-3-methoxypropionamide hydrochloride (60.00 g, 138 mmol), potassium carbonate (60 g), water (300 mL), and ethanol (900 mL) was heated at reflux for 16 hours and then concentrated under reduced pressure. The precipitated solid was isolated by filtration, washed sequentially with water and methanol, and dried to provide a brown solid. This material was dissolved in a 3/1 mixture of chloroform/methanol and decolorized with activated charcoal to provide 38.5 g of 1-[7-bromo-2-(2-methoxyethyl)-1H-imidazo[4,5-c][1,5]naphthyridin-1-yl]-2-methylpropan-2-ol as a white solid, mp 125° C. Anal. calcd for C₁₆H₁₉BrN₄O₂: % C, 50.67; % H, 5.05; % N, 14.77. Found: % C, 50.86; % H, 4.94; % N, 15.01.

Part I

3-Chloroperoxybenzoic acid (34.77 g of 75%, 151 mmol) was added to a solution of 1-[7-bromo-2-(2-methoxyethyl)-1H-imidazo[4,5-c][1,5]naphthyridin-1-yl]-2-methylpropan-2-ol (38.2 g, 101 mmol) in dichloromethane (450 mL) and the reaction mixture was stirred for 3 hours. The reaction mixture was diluted with dichloromethane (200 mL), washed sequentially with 4% aqueous sodium carbonate (2×150 mL) and brine (1×150 mL), and concentrated under reduced pressure to provide the N-oxide derivative. The N-oxide derivative was combined with dichloromethane (450 mL) and concentrated ammonium hydroxide (200 mL) and the mixture was cooled in an ice bath. Para-Toluenesulfonyl chloride (24 g) was added in portions. After the addition was complete the ice bath was removed and the reaction mixture was stirred at ambient temperature for 16 hours. The reaction mixture was diluted with dichloromethane (200 mL). Suspended solids were isolated by filtration, washed with water, and dried to provide 7.60 g of 1-[4-amino-7-bromo-2-(2-methoxyethyl)-1H-imidazo[4,5-c][1,5]naphthyridin-1-yl]-2-methylpropan-2-ol as an off white solid, mp 210-211° C. Anal. calcd for C₁₆H₂₀BrN₅O₂: % C, 48.74; % H, 5.11; % N, 17.76. Found: % C, 48.63; % H, 5.10; % N, 17.80.

Part J

A Parr vessel was charged with 10% Pd/C (0.6 g) and a suspension of 1-[4-amino-7-bromo-2-(2-methoxyethyl)-1H-imidazo[4,5-c][1,5]naphthyridin-1-yl]-2-methylpropan-2-ol (4.0 g) in acetonitrile (150 mL) and methanol (50 mL). The vessel was placed under hydrogen pressure (50 psi, 3.4×10⁵Pa) for 3 hours. The reaction mixture was diluted with 1/1 chloroform/methanol (100 mL), filtered through a layer of CELITE filter aid, and concentrated under reduced pressure. The residue was triturated with acetonitrile to provide 3.55 g of 1-[4-amino-2-(2-methoxyethyl)-1H-imidazo[4,5-c][1,5]naphthyridin-1-yl]-2-methylpropan-2-ol hydrobromide as a white powder, mp 234-235° C. Anal. calcd for C₁₆H₂₂BrN₅O₂: % C, 48.49; % H, 5.60; % N, 17.67. Found: % C, 48.64; % H, 5.69; % N, 17.62.

Part K

A solution of boron tribromide in dichloromethane (22.71 mL of 1 M) was added to a chilled (0° C.) suspension of 1-[4-amino-2-(2-methoxyethyl)-1H-imidazo[4,5-c][1,5]naphthyridin-1-yl]-2-methylpropan-2-ol hydrobromide (3.00 g, 7.57 mmol) in dichloromethane (100 mL). The reaction mixture was allowed to come to ambient temperature with stirring for 16 hours. Methanol (30 mL) and hydrochloric acid (30 mL of 6 N) were added and the reaction mixture was heated at reflux for 2.5 hours. The reaction mixture was made basic with sodium hydroxide and the layers were separated. The aqueous layer was extracted with dichloromethane (100 mL). The extract was washed sequentially with water and brine, dried over magnesium sulfate, and then concentrated under reduced pressure to provide a pink solid. This material was crystallized from acetonitrile to provide 0.68 g of 1-[4-amino-2-(2-hydroxyethyl)-1H-imidazo[4,5-c][1,5]naphthyridin-1-yl]-2-methylpropan-2-ol. The aqueous layer was combined with the water and brine washings and allowed to stand overnight. A precipitate was isolated by filtration, washed with water, and dried under vacuum at 95° C. for 3 hours to provide 1.16 g of 1-[4-amino-2-(2-hydroxyethyl)-1H-imidazo[4,5-c][1,5]naphthyridin-1-yl]-2-methylpropan-2-ol as a pink crystalline solid, mp 194-195° C. Anal. calcd for C₁₅H₁₉N₅O₂: % C, 59.79; % H, 6.36; % N, 23.24. Found: % C, 59.51; % H, 6.59; % N, 23.34.

Examples 150-155
Preparation of N-[2-(4-amino-2-ethoxymethyl-1H-imidazo[4,5-c][1,5]naphthyridin-1-yl)-1,1-dimethylethyl]-2-methylpropionamide

Triethylamine (556 μL, 4.00 mmol) and isobutyryl chloride (230 μL, 2.20 mmol) were added sequentially to a chilled (0° C.) solution of 1-(2-amino-2-methylpropyl)-2-ethoxymethyl-1H-imidazo[4,5-c][1,5]naphthyridin-4-amine (628 mg, 2.00 mmol) in dichloromethane (20 mL). The reaction mixture was allowed to warm slowly to ambient temperature overnight. The reaction mixture was quenched with aqueous saturated sodium bicarbonate and diluted with dichloromethane (50 mL). The organic layer was separated, washed sequentially with water and brine, dried over sodium sulfate, filtered, and then concentrated under reduced pressure to an amber foam. This material was dissolved in hot propyl acetate (10 mL) and then allowed to cool overnight. Hexanes were added and the now cloudy solution was heated until clear and then allowed stand until crystals formed. The solvent was removed by pipette. The crystals were rinsed with cold propyl acetate/hexanes and then dried under high vacuum at 70° C. to provide 464 mg of N-[2-(4-amino-2-ethoxymethyl-1H-imidazo[4,5-c][1,5]naphthyridin-1-yl)-1,1-dimethylethyl]-2-methylpropionamide as an off white crystalline solid, mp 154.5-155.5° C. Anal. calcd for C₂₀H₂₈N₆O₂: % C, 62.48; % H, 7.34; % N, 21.86. Found: % C, 62.14; % H, 7.62; % N, 21.71.

Preparation of 1-[2-(4-amino-2-ethoxymethyl-1H-imidazo[4,5-c][1,5]naphthyridin-1-yl)-1,1-dimethylethyl]-3-(1-methylethyl)urea

Under a nitrogen atmosphere, isopropyl isocyanate (206 μL, 2.10 mmol) was added to a chilled (0° C.) solution of 1-(2-amino-2-methylpropyl)-2-ethoxymethyl-1H-imidazo[4,5-c][1,5]naphthyridin-4-amine (628 mg, 2.00 mmol) in dichloromethane (20 mL). The reaction mixture was allowed to warm slowly to ambient temperature overnight. The resulting precipitate was isolated by filtration and then dried under vacuum at 70° C. to provide 669 mg of 1-[2-(4-amino-2-ethoxymethyl-1H-imidazo[4,5-c][1,5]naphthyridin-1-yl)-1,1-dimethylethyl]-3-(1-methylethyl)urea as white powder, mp 172.5-173.5° C. Anal. calcd for C₂₀H₂₉N₇O₂: % C, 60.13; % H, 7.32; % N, 24.54. Found: % C, 59.88; % H, 7.55; % N, 24.51.

A solution of boron tribromide in dichloromethane (about 4 eq of 1 M) was added to a tube containing a chilled (0° C.) solution of a compound of Formula Xb (25 mg, 1 eq) in dichloromethane (1 mL). The tube was vortexed, maintained at 0° C. for 0.5 hour, and then shaken overnight at ambient temperature. The reaction mixture was diluted with methanol (1 mL) and hydrochloric acid (500 μL of 6 N), vortexed, and then the solvents were removed by vacuum centrifugation. The compounds were purified by preparative high performance liquid chromatography (prep HPLC) using a Waters FractionLynx automated purification system. The prep HPLC fractions were analyzed using a Waters LC/TOF-MS, and the appropriate fractions were centrifuge evaporated to provide the trifluoroacetate salt of the desired compound. Reversed phase preparative liquid chromatography was performed with non-linear gradient elution from 5-95% B where A is 0.05% trifluoroacetic acid/water and B is 0.05% trifluoroacetic acid/acetonitrile. Fractions were collected by mass-selective triggering. Table 4 shows the structure of the starting material, the structure of the resulting compound, and the observed accurate mass for the isolated trifluoroacetate salt.

TABLE 4

Measured Mass

Example
R₁
(M + H)

150

288.1440

151

365.1378

152

397.2348

153

287.1607

154

357.2055

155

372.2157

Examples 156-161

A solution of boron tribromide in heptane (400 μL of 1 M) was added to a tube containing a chilled (0° C.) solution of a compound of Formula Xc (about 25 mg) in dichloromethane (1 mL). The tube was vortexed, maintained at 0° C. for 0.5 hour, and then shaken overnight at ambient temperature. The reaction mixture was diluted with methanol (1 mL) and hydrochloric acid (250 μL of 6 N), vortexed, and then the solvents were removed by vacuum centrifugation. The compounds were purified by preparative high performance liquid chromatography (prep HPLC) using a Waters FractionLynx automated purification system. The prep HPLC fractions were analyzed using a Waters LC/TOF-MS, and the appropriate fractions were centrifuge evaporated to provide the trifluoroacetate salt of the desired compound. Reversed phase preparative liquid chromatography was performed with non-linear gradient elution from 5-95% B where A is 0.05% trifluoroacetic acid/water and B is 0.05% trifluoroacetic acid/acetonitrile. Fractions were collected by mass-selective triggering. Table 5 shows the structure of the starting material, a reference for the starting material, the structure of the resulting compound, and the observed accurate mass for the isolated trifluoroacetate salt.

TABLE 5

Reference

Measured

Example
Formula Xc
R₁
R₃
Mass (M + H)

156
U.S. PatentPublication2004/0147543Example 206

430.2227

157
U.S. PatentPublication2004/0147543Example 136

377.1985

158
U.S. PatentPublication2004/0147543Example 145

362.2008

159
U.S. PatentPublication2004/0147543Example 146

392.2104

160
U.S. PatentPublication2004/0147543Example 183

431.2209

161
U.S. PatentPublication2004/0147543Example 184

431.2220

Examples 162-186
Part A

1-(4-Amino-7-bromo-2-ethoxymethyl-1H-imidazo[4,5-c]quinolin-1-yl)-2-methylpropan-2-ol (2 g, U.S. Patent Publication 2004/0147543 Example 125) was dissolved in 7:3 volume:volume chloroform:methanol (100 mL). Aliquots (2 mL, 1.0 eq.) were added to test tubes and the solvent was removed by vacuum centrifugation. A tube was charged with a boronic acid (1.1 eq) from the table below. n-Propanol (1.6 mL) was added to each tube, the tube was purged with nitrogen, and then sonicated until the contents were well mixed. Each tube was then charged sequentially with 150 μL of a solution of palladium (II) acetate in toluene (60 mg of palladium (II) acetate dissolved in 15 mL of toluene), 600 μL of 2 M aqueous sodium carbonate solution, 113 μL of water, and 53 μL of a 15 mole % solution of triphenylphosphine in n-propanol. The tubes were purged with nitrogen and then heated at 80° C. overnight.

The reaction mixtures were purified by solid phase extraction. Sufficient hydrochloric acid (1 N) was added to each reaction mixture to adjust the pH to <5. Each reaction mixture was loaded onto a cartridge (Waters Oasis Samples Extraction Cartridges MCX 6 cc). Methanol (5 mL) was added to each cartridge. The cartridge was placed in a clean test tube. The cartridge was eluted with two successive 5 mL portions of 1 N ammonia in methanol. The solvent was removed by vacuum centrifugation.

Part B

Dichloromethane (1 mL) was added to each tube, the tube was sonicated to dissolve the solids, and then the tube was chilled to 0° C. in an ice bath. A solution of boron tribromide in heptane (600 μL of 1 M) was added to each tube. The tube was vortexed, maintained at 0° C. for 0.5 hour, and then shaken overnight at ambient temperature. The solvents were removed by vacuum centrifugation. Methanol (1 mL) and hydrochloric acid (1 mL of 6 N) were added to each tube, the tubes were vortexed, and then the solvents were removed by vacuum centrifugation. The compounds were purified as described above for Examples 156-161. Table 6 shows the boronic acid, the structure of the resulting compound, and the observed accurate mass for the isolated trifluoroacetate salt.

TABLE 6

Measured

Mass

Example
Reagent
R₃
(M + H)

162
Phenylboronic acid

363.1847

163
Pyridine-3-boronic acid

364.1779

164
3-Methylphenylboronic acid

377.2001

165
4-Methylphenylboronic acid

377.1979

166
o-Tolylboronic acid

377.1990

167
(2-Hydroxyphenyl)boronic acid

379.1776

168
3-Hydroxyphenylboronic acid

379.1755

169
3,5-Dimethylphenylboronic acid

391.2130

170
4-(Hydroxymethyl)phenylboronic acid

393.1935

171
3-Chlorophenylboronic acid

397.1432

172
2-Chlorophenylboronic acid

397.1447

173
4-Chlorophenylboronic acid

397.1431

174
2,4-Difluorophenylboronic acid

399.1642

175
Benzol[b]furan-2-boronic acid

403.1812

176
(3-Aminocarbonylphenyl)boronic acid

406.1889

177
4-(N,N-Dimethylamino)phenylboronicacid

406.2255

178
(3-Aminomethylphenyl)boronic acidhydrochloride

392.2108

179
3,4-Dichlorophenylboronic acid

431.1061

180
4-(Ethylsulfonyl)phenylboronic acid

455.1771

181
3-(Methylsulfonylamino)phenylboronicacid

456.1727

182
3-(Pyrrolidine-1-carbonyl)phenylboronic acid

460.2364

183
4-(Pyrrolidine-1-carbonyl)phenylboronic acid

460.2395

184
3-(Butylaminocarbonyl)phenylboronicacid

462.2488

185
3-(Isobutylaminocarbonyl)phenylboronicacid

462.2527

186
4′-(4,4,5,5-Tetramethyl-1,3,2-dioxaborolan-2-yl)acetanilinde

420.2022

Example 187
1-[4-Amino-2-hydroxymethyl-7-(thiazol-4-ylmethoxy)-1H-imidazo[4,5-c]quinolin-1-yl]-2-methylpropan-2-ol

Under a nitrogen atmosphere, a solution of 1-[4-amino-2-ethoxymethyl-7-(thiazol-4-ylmethoxy)-1H-imidazo[4,5-c]quinolin-1-yl]-2-methylpropan-2-ol (400 mg, 0.94 mmol, which can be prepared as described in International Application No. PCT/US04/28021 Example 137) in dichloromethane (50 mL) was cooled to 0° C. in an ice bath. A solution to of boron tribromide in dichloromethane (3.76 mL of 1.0 M) was added slowly. The reaction mixture was allowed to slowly warm to ambient temperature overnight. The reaction mixture was diluted with hydrochloric acid (20 mL of 6 N) and stirred for 30 minutes. The layers were separated. The organic layer was washed with 6 N hydrochloric acid (3×20 mL) and then discarded. The aqueous layer was made basic by the addition of solid potassium carbonate. A precipitate was isolated by filtration, dissolved in hot chloroform, and then purified by prep HPLC (HORIZON HPFC system eluting with 0-10% CMA in chloroform over 192 mL and then with 10-40% CMA in chloroform over 1400 mL) to provide a solid. This material was crystallized from acetonitrile to provide 181 mg of 1-[4-amino-2-hydroxymethyl-7-(thiazol-4-ylmethoxy)-1H-imidazo[4,5-c]quinolin-1-yl]-2-methylpropan-2-ol as a white solid, mp 260-262° C. Anal. calcd for C₁₉H₂₁N₅O₃S: % C, 56.81; % H, 5.41; % N, 17.26. Found: % C, 56.82; % H, 5.54; % N, 17.23.

Example 188
[4-Amino-7-pyridin-3-yl-1-(tetrahydro-2H-pyran-4-ylmethyl)-1H-imidazo[4,5-c]quinolin-2-yl]methanol

Part A

To a mixture of 1-tetrahydro-2H-pyran-4-ylmethanamine HCl (19 g, 120 mmol), dichloromethane (626 mL), and triethyl amine (43.7 mL, 313 mmol) was added 4-chloro-3-nitroquinoline at 0° C. The resulting bright yellow solution was stirred at ambient temperature for 18 hours. The reaction was then concentrated under reduced pressure. The resulting solid was stirred in water (100 mL) and filtered to give 43 g of 7-bromo-3-nitro-N-(tetrahydro-2H-pyran-4-ylmethyl)quinolin-4-amine as a yellow powder.

Part B

7-Bromo-3-nitro-N-(tetrahydro-2H-pyran-4-ylmethyl)quinolin-4-amine (20 g, 55 mmol) was dissolved in a mixture of acetonitrile (500 mL) and isopropyl alcohol (50 mL) and the solution was placed in a pressure bottle. Platinum on carbon (5%, 2 g) was then added and the reaction mixture was shaken under H₂at 48 PSI (3.3×10⁵Pa). After 2 hours, the reaction mixture was filtered through a pad of CELITE filter agent. The pad was rinsed with acetonitrile and the combined filtrates were concentrated under reduced pressure to give 7-bromo-N-(tetrahydro-2H-pyran-4-ylmethyl)quinoline-3,4-diamine which was carried forward without further purification assuming quantitative yield.

Part C

Chloroacetyl chloride (5.2 mL, 65 mmol) was added to 7-bromo-N⁴-(tetrahydro-2H-pyran-4-ylmethyl)quinoline-3,4-diamine (55 mmol) dissolved in 273 mL of dichloromethane at 0° C. A solid formed after adding half of the chloroacetyl chloride at which point additional dichloromethane (100 mL) was added. The reaction was stirred for 1 hour at ambient temperature. The yellow suspension was quenched first with aqueous saturated sodium bicarbonate followed by 50% aqueous sodium hydroxide until a pH of 14 was reached. Filtration provided 10 g of N-{7-bromo-4-[(tetrahydro-2H-pyran-4-ylmethyl)amino]quinolin-3-yl}-2-chloroacetamide as a tan solid. The filtrate was placed in a separatory funnel and the layers were separated. The aqueous layer was extracted with additional dichloromethane. The combined organic extracts were combined, dried over sodium sulfate, filtered, and concentrated under reduced pressure to afford additional N-{7-bromo-4-[(tetrahydro-2H-pyran-4-ylmethyl)amino]quinolin-3-yl}-2-chloroacetamide as a yellow oil. The yellow oil was carried forward without further purification assuming a 50% yield (27.3 mmol). The oil was combined with ethanol (100 mL) and triethylamine (7.5 mL, 54 mmol). The resulting yellow solution was refluxed for 2 hours. The reaction was cooled to ambient temperature and the solvent was removed under reduced pressure to provide 7-bromo-2-(chloromethyl)-1-(tetrahydro-2H-pyran-4-ylmethyl)-1H-imidazo[4,5-c]quinoline as a brown oil that was used without further purification assuming quantitative yield.

Part D

Potassium acetate (5.3 g, 55 mmol) was added to 7-bromo-2-(chloromethyl)-1-(tetrahydro-2H-pyran-4-ylmethyl)-1H-imidazo[4,5-c]quinoline (27.3 mmol) dissolved in dimethylformamide (100 mL). The resulting suspension was stirred at 90° C. for 1 hour. The reaction was cooled to ambient temperature and water (200 mL) was added. The aqueous layer was extracted with chloroform. The combined organic extracts were combined, dried over sodium sulfate, filtered, and concentrated under reduced pressure to afford an orange oily solid. Chromatography (SiO₂, O-30% 80/18/2 v/v/v CHCl₃/CH₃OH/concentrated NH₄OH (CMA)/CHCl₃) gave material that was stirred in acetonitrile and filtered to provide 2.3 g of [7-bromo-1-(tetrahydro-2H-pyran-4-ylmethyl)-1H-imidazo[4,5-c]quinolin-2-yl]methyl acetate as a tan solid.

Part E

3-Chloroperoxybenozic acid (2.4 g, 50% pure, 7.0 mmol) was added to a mixture of [7-bromo-1-(tetrahydro-2H-pyran-4-ylmethyl)-1H-imidazo[4,5-c]quinolin-2-yl]methyl acetate (2.3 g, 5.4 mmol) and chloroform (27 mL) at ambient temperature. The reaction was stirred at this temperature for 18 hours. Saturated aqueous sodium bicarbonate (50 mL) and water (50 mL) were then added to the reaction and the layers were separated. The aqueous layer was extracted with additional dichloromethane. The organic layers were combined, dried over sodium sulfate, and concentrated under reduced pressure to a dark oil. This oil was dissolved in methanol (27 mL) and to this solution was added 15 M ammonium hydroxide (3.6 mL, 54 mmol) and benzene sulfonyl chloride (2.9 mL, 23 mmol). The resulting reaction mixture was stirred at ambient temperature for 2 hours before adding additional 15 M ammonium hydroxide (3.6 mL, 54 mmol) and benzene sulfonyl chloride (2.9 mL, 23 mmol). The reaction was stirred 18 hours. The reaction was then concentrated under reduced pressure and diluted with saturated aqueous sodium bicarbonate and chloroform. A suspension resulted that was filtered to afford a solid that was stirred with saturated aqueous sodium bicarbonate and filtered to give 1.1 g of [4-amino-7-bromo-1-(tetrahydro-2H-pyran-4-ylmethyl)-1H-imidazo[4,5-c]quinolin-2-yl]methanol as a white solid.

Part F

To a mixture of [4-amino-7-bromo-1-(tetrahydro-2H-pyran-4-ylmethyl)-1H-imidazo[4,5-c]quinolin-2-yl]methanol (500 mg, 1.28 mmol), 3-pyridyl boronic acid (233 mg, 1.90 mmol), potassium carbonate (579 mg, 4.20 mmol), dimethoxyethane (5 mL), and water (2.5 mL) under a nitrogen atmosphere was added Pd(PPh₃)₂Cl₂(18 mg, 0.026 mmol). The resulting suspension was refluxed for 2 hours. The reaction was cooled to ambient temperature. The reaction mixture was diluted with chloroform and placed directly onto a silica gel column. Chromatography (SiO₂, 0-40% CMA/CHCl₃) gave material that was stirred in methanol and filtered to provide 263 mg of [4-amino-7-pyridin-3-yl-1-(tetrahydro-2H-pyran-4-ylmethyl)-1H-imidazo[4,5-c]quinolin-2-yl]methanol as tan crystals, m.p. 260-262° C. MS (APCI) m/z 500.3 (M+H)⁺; Anal. calcd for C₂₂H₂₃N₅O₂: C, 67.85; H, 5.95; N, 17.98. Found: C, 67.49; H, 5.87; N, 17.83.

Examples 189-207

The compounds in the table below were prepared according to the following general procedure. The ether analog was dissolved or suspended in a solvent such as dichloromethane and the reaction mixture was stirred at 0° C. or at ambient temperature. Boron tribromide (2.5-10 equivalents, 1 M solution in dichloromethane) was added dropwise to the reaction mixture. The reaction was stirred at ambient temperature for 4 h -6 days after which it was quenched by the careful addition of methanol or water and the solvent was removed under reduced pressure. The product was isolated by a procedure similar to that described below. The residue was combined with 2-6 M hydrochloric acid, heated to 50° C., and stirred for 1-2 hours. The resulting solution was cooled (ice bath) and then free-based (pH 9) with the addition of 2-6 M aqueous sodium hydroxide. The desired material was extracted from the aqueous using an organic solvent such as dichloromethane, ethyl acetate, or chloroform. The organic layer was separated, dried (MgSO₄), filtered, and the solvent was evaporated under reduced pressure to afford the crude product. The final compound was isolated by prep HPLC (ISCO Combiflash Separation System or Analogix Purification System).

Example
Structure
Analytical Data

189

Off-white needles, mp 180-182° C.Anal. calcd for C₂₁H₂₃N₅O₃•2.60H₂O: C,57.29; H, 6.46; N, 15.91. Found: C, 57.32;H, 6.15; N, l5.73; MS (APCI) m/z 394(M + H)⁺.

190

Off-white needles, mp 196-198° C.Anal. calcd for C₂₃H₂₆N₆O₃S: C, 59.21; H,5.62; N, 18.01. Found: C, 59.16; H, 5.84;N, 17.98; MS (APCI) m/z 467 (M + H)⁺.

191

Off-white needles, mp 154-157° C.Anal. calcd for C₂₆H₃₀N₆O₂•0.25H₂O: C,67.44; H, 6.64; N, 18.15. Found: C, 67.48;H, 6.55; N, 18.00; MS (APCI) m/z 459(M + H)⁺.

192

Off-white needles, mp 182-184° C.Anal. calcd for C₂₆H₃₁N₇O₂: C, 65.94; H,6.60; N, 20.70. Found: C, 65.70; H, 6.49;N, 20.39); MS (APCI) m/z 474 (M + H)⁺.

193

Beige needles, mp 111-114° C.Anal. calcd for C₂₀H₂₀FN₅O₂•2.0 H₂O: C,57.55; H, 5.79; N, 16.78. Found: C, 57.33;H, 5.57; N, 16.76MS (APCI) m/z 382 (M + H)⁺

194

Off-white solid, mp 188-190° C.Anal. calcd for C₂₁H₂₄N₆O₃S•1.70H₂OC: 53.53, H: 5.86, N: 17.84. Found:C: 53.23, % H: 5.62, N: 17.81.MS (APCI) m/z 459 (M + H)⁺

195

Green solid, mp 206-209° C.Anal. calcd for C₂₄H₂₉N₇O₂•0.27H₂OC: 63.72, H: 6.58, N: 21.67.Found: C: 63.97, H: 6.26, N: 21.64.MS (APCI) m/z 448 (M + H)⁺

196

Off-white solid, mp 211-212° C.Anal. calcd for C₂₄H₂₈N₆O₂•0.25H₂OC: 65.96, H: 6.57, N: 19.23.Found: C: 65.52H: 6.38, N: 19.38MS (APCI) m/z 433 (M + H)⁺

197

Yellow solid, mp 225-227° C.Anal. calcd for C₂₆H₃₁N₇O₂•0.38H₂OC: 65.00, H: 6.66, N: 20.41.Found: C: 65.26, H: 6.53, N: 20.42.MS (APCI) m/z 474 (M + H)⁺

198

White solid, mp 241-242° C.Anal. calcd for C₂₆H3₀N₆O₂C: 68.10, H: 6.59, N: 18.33.Found: C: 67.85, H: 6.48, N: 18.32.MS (APCI) m/z 459 (M + H)⁺

199

White solid, mp 225-227° C.Anal. calcd for C₂₄H₂₈N₆O₂•0.38H₂OC: 65.61, H: 6.60, N: 19.13.Found: C: 65.19, H: 6.74, N: 18.96.MS (APCI) m/z 433 (M + H)⁺

200

White solid, mp >300° C.Anal. calcd for C₂₄H₂₈N₆O₄S•HBr•0.2H₂O:C: 49.61; H, 5.10; N, 14.46, Found: C,49.26; H, 4.84; N, 14.29MS (APCI) m/z 497 (M + H)⁺

201

Tan solid, mp >300° C.Anal. calcd for C₂₇H₃₂N₆O₃•HBr: C, 56.94;H, 5.84; N, 14.76. Found: C, 56.66; H,5.69; N, 14.63.MS (APCI) m/z 489 (M + H)⁺

202

Off-white solid, mp >300° C.Anal. calcd for C₂₇H₃₃N₇O₃•HBr: C, 55.14;H, 5.90; N, 16.67. Found: C, 54.86; H,5.60; N, 16.64.MS (APCI) m/z 504 (M + H)⁺

203

Off white needles, mp 218-221° C.Anal. calcd for C₂₆H₂₉N₅O₂•1.25 H₂O: C,67.00; H, 6.81; N, 15.03. Found: C, 67.04;H, 6.78, N, 14.90.MS (APCI) m/z 444 (M + H)⁺

204

Off white solid, mp >250° C.Anal. calcd for C₂₅H₂₇N₅O₃•0.75 H₂O: C,65.41; H, 6.26; N, 15.26. Found: C, 65.48;H, 6.40; N, 15.07.MS (APCI) m/z 446 (M + H)⁺

205

Off-white solid, mp 166-170° C.Anal. calcd for C₂₄H₂₇N₅O₂•0.9 H₂O: C,66.46; H, 6.69; N, 16.15. Found: C, 66.09;H, 6.73; N, 15.97.MS (APCI) m/z 4l8 (M + H)⁺

206

Off-white solid, mp 260-264° C.Anal. calcd for C₂₉H₃₃N₅O₃•0.6 H₂O•1.0HCl: C, 63.69; H, 6.49; N, 12.81. Found:C, 63.37; H, 6.23; N, 12.62.MS (APCI) m/z 500 (M + H)⁺

207

Off-white needles, mp 141-143° C.Anal. calcd for C₂₀H₂₁N₅O₂•1.00CH₄O•1.0H₂O: C, 61.15 H, 6.35 N, 16.98. Found:C, 61.15 H, 6.06 N, 17.34.MS (APCI) m/z 364 (M + H)⁺

Examples 208-318
Part A

A solution of 1-(4-aminobutyl)-2-ethoxymethyl-7-(pyridin-3-yl)-1H-imidazo[4,5-c]quinoline-4-amine (43 mg, 0.10 mmol, 1 eq, U.S. Patent Application Publication 2004/0147543, Example 372) and triethylamine (5 eq) in chloroform (1 mL) was added to a tube containing a reagent (1.1 eq) from the table below. The reaction mixture was vortexed overnight and then purified by solid-supported liquid-liquid extraction according to the following procedure. The reaction mixture was loaded onto diatomaceous earth that had been equilibrated with 1 N sodium hydroxide (600 μL) for about 20 minutes. After 10 minutes chloroform (300 μL) was added to elute the product from the diatomaceous earth into a well of a collection plate. After an additional 10 minutes the process was repeated with additional chloroform (500 μL). The solvent was then removed by vacuum centrifugation.

Part B

The material from Part A was dissolved in dichloromethane (600 μL) and the solution was cooled to 0° C. Boron tribromide (400 μL of 1 M in dichloromethane) was added, the reaction mixture was vortexed, chilled for 15 minutes, and then vortexed at ambient temperature overnight. The solvent was removed by vacuum centrifugation. Methanol (300 μL) and 6 N hydrochloric acid (300 μL) were added and the reaction mixture was vortexed for 10 minutes. The solvent was removed by vacuum centrifugation. The compounds were purified as described above for Examples 156-161. The table below shows the reagent used for each example, the structure of the resulting compound, and the observed accurate mass for the isolated trifluoroacetate salt.

Measured Mass

Example
Reagent
R
(M + H)

208
None

363.1964

209
Propionyl chloride

419.2168

210
Cyclopropanecarbonylchloride

431.2213

211
Butyryl chloride

433.2345

212
Isobutyryl chloride

433.2346

213
Methoxyacetyl chloride

421.1982

214
Cyclobutanecarbonylchloride

445.2338

215
Isovaleryl chloride

447.2536

216
Cyclohexanecarbonylchloride

473.2679

217
Phenylacetyl chloride

481.2368

218
4-Cyanobenzoyl chloride

492.2143

219
3-Methoxybenzoylchloride

483.2121

220
p-Anisoyl chloride

483.2115

221
2-Chlorobenzoylchloride

501.1813

222
3-Chlorobenzoylchloride

501.1812

223
Nicotinoyl chloridehydrochloride

468.2122

224
Picolinoyl chloridehydrochloride

468.2124

225
1-Propanesulfonylchloride

469.2039

226
Dimethylsulfamoylchloride

470.1961

227
1-Butanesulfonylchloride

483.2160

228
3-Methylbenzenesulfonylchloride

517.2044

229
o-Toluenesulfonylchloride

517.2071

300
p-Toluenesulfonylchloride

517.2020

301
2-Fluorobenzenesulfonylchloride

521.1786

302
3-Cyanobenzenesulfonylchloride

528.1805

303
3-Methoxybenzenesulfonylchloride

519.1829

304
4-Methoxybenzenesulfonylchloride

519.1799

305
3-Pyridinesulfonylchloride hydrochloride

504.1852

306
Ethyl isocyanate

434.2307

307
Isopropyl isocyanate

448.2498

308
n-Propyl isocyanate

448.2448

309
Cyclopentyl isocyanate

474.2629

310
Phenyl isocyanate

482.2338

311
Cyclohexyl isocyanate

488.2759

312
2-Fluorophenylisocyanate

500.2209

313
3-Fluorophenylisocyanate

500.2206

314
4-Fluorophenylisocyanate

500.2209

315
(R)-(+)-alpha-Methylbenzyl isocyanate

510.2580

316
(S)-(−)-alpha-Methylbenzyl isocyanate

510.2588

317
1-Piperidinecarbonylchloride

474.2606

318
4-Methyl-1-piperazinecarbonylchloride

489.2725

Examples 319-345

The compounds in the table below were prepared and purified according to the general method of Examples 162-186 using N-{4-[4-amino-7-bromo-2-ethoxymethyl-1H-imidazo[4,5-c]quinolin-1-yl]butyl}methanesulfonamide (U.S. Patent Application Publication 2004/0147543, Example 612) in lieu of 1-(4-amino-7-bromo-2-ethoxymethyl-1H-imidazo[4,5-c]quinolin-1-yl)-2-methylpropan-2-ol. Prior to purification by solid phase extraction, the reaction mixture for Example 345 was combined with water (500 μL), glacial acetic acid (500 μL), and tetrahydrofuran (500 μL) and then heated at 60° C. for 2 hours. The table below shows the boronic acid, the structure of the resulting compound, and the observed accurate mass for the isolated trifluoroacetate salt.

Measured

Mass

Example
Reagent
R
(M + H)

319
Phenylboronic acid

440.1745

320
Pyridine-3-boronic acid

441.1745

321
Pyridine-4-boronic acid

441.1679

322
Thiophene-3-boronic acid

446.1307

323
2-Fluorophenylboronic acid

458.1668

324
3-Fluorophenylboronic acid

458.1671

325
4-Fluorophenylboronie acid

458,1674

326
4-Cyanophenylboronic acid

465.1684

327
3-(Hydroxymethyl)phenylboronic acid

470.1882

328
4-(Hydroxymethyl)phenylboronic acid

470.1909

329
3-Chlorophenylboronic acid

474.1408

330
2-Chlorophenylboronic acid

474.1366

331
4-Chlorophenylboronic acid

474.1384

332
(2-Aminocarbonylphenyl)boronic acid

483.1796

333
(3-Aminocarbonylphenyl)boronic acid

483.1812

334
(2-Acetylaminophenyl)boronic acid

497.1938

335
[3-(3-Hydroxypropyl)phenyl]boronicacid

498.2136

336
3,4-Dichlorophenylboronic acid

508.0989

337
3-(N-Isopropylaminocarbonyl)phenylboronicacid

525.2331

338
3-(N-Propylaminocarbonyl)phenylboronicacid

525.2284

339
3-(Methylsulfonylamino)phenylboronicacid

533.1659

340
3-(Pyrrolidine-1-carbonyl)phenylboronic acid

537.2320

341
4-(Pyrrolidine-1-carbonyl)phenylboronic acid

537.2271

342
3-(Isobutylaminocarbonyl)phenylboronicacid

539.2418

343
4-(Isobutylaminocarbonyl)phenylboronicacid

539.2429

344
3-(Piperidine-1-carbonyl)phenylboronic acid

551.2483

345
5-tert-butyldimethylsilanyloxy-methyl)pyridine-3-boronic acid

471.1819

Examples 346-362

The compounds in the table below were prepared according to the following method. A test tube containing a solution of the corresponding ether analog (ethoxymethyl or methoxyethyl) in dichloromethane (1 mL) was cooled to 0° C. in an ice bath. Boron tribromide (4 eq of 1 M in dichloromethane) was added. The tube was vortexed, maintained at 0° C. for 0.5 hr, and then stirred at ambient temperature for 9 hours. Methanol (1 mL) and 6 N hydrochloric acid (500 μL) were added and the tube was vortexed for 5 minutes. The solvent was removed by vacuum centrifugation. The compounds were purified as described above for Examples 156-161. The table below shows a reference for the starting ether, the structure of the resulting compound, and the observed accurate mass for the isolated trifluoroacetate salt.

Reference for ether

Measured Mass

Example
starting material
R₁
R₂
R₃
(M + H)

346
Example 102

347.1904

347
Example 111

372.1819

348
Example 201

440.1755

349
Example 113

348.1810

350
Example 194

458.2540

351
Example 139

470.1832

352
Example 152

466.1897

353
Example 180

502.2554

354
Example 129

460.2326

355
Example 130

476.2285

356
Example 376

469.2024

357
Example 438

388.2130

358
Example 492

467.1852

359
Example 488

366.1574

360
Example 422

433.2374

361
Example 480

482.1815

362
*

476.2383

*Although not specifically exemplified, the compound is readily prepared using the disclosed synthetic methods. All references are to U.S. Patent Application Publication 2004/0147543.

Example 363
[4-Amino-7-[3-(pyrrolidin-1-ylcarbonyl)phenyl]-1-(tetrahydro-2H-pyran-4-ylmethyl)-1H-imidazo[4,5-c]quinolin-2-yl]methanol

To a mixture of [4-amino-7-bromo-1-(tetrahydro-2H-pyran-4-ylmethyl)-1H-imidazo[4,5-c]quinolin-2-yl]methanol (400 mg, 1.00 mmol), 3-pyrrolidinylcarbonyl phenyl boronic acid (328 mg, 1.50 mmol), potassium carbonate (455 mg, 3.30 mmol), dimethoxyethane (4 mL), and water (2 mL) under a nitrogen atmosphere was added Pd(PPh₃)₂Cl₂(14 mg, 0.02 mmol). The resulting suspension was refluxed for 18 hours. The reaction was cooled to ambient temperature. The reaction mixture was diluted with water and extracted with chloroform. The organic layer was dried over sodium sulfate, filtered and concentrated under reduced pressure. Chromatography (SiO₂, 0-40% CMA/CHCl₃) gave material that was stirred in acetonitrile and filtered to provide 100 mg of [4-amino-7-[3-(pyrrolidin-1-ylcarbonyl)phenyl]-1-(tetrahydro-2H-pyran-4-ylmethyl)-1H-imidazo[4,5-c]quinolin-2-yl]methanol as a white powder, m.p. 281-284° C. MS (APCI) m/z 486.3 (M+H)⁺; Anal. calcd for C₂₈H₃₁N₅O₃: C, 69.26; H, 6.43; N, 14.42. Found: C, 68.99; H, 6.16; N, 14.46.

Example 364
{4-Amino-1-[2,2-dimethyl-3-(methylsulfonyl)propyl]-1H-imidazo[4,5-c]quinolin-2-yl}methanol

To a suspension of 1-[2,2-dimethyl-3-(methylsulfonyl)propyl]-2-(ethoxymethyl)-1H-imidazo[4,5-c]quinolin-4-amine (0.4 g, 1.02 mmol) in dichloromethane (5 mL) was added boron tribromide (5.1 mL, 1M solution in dichloromethane). An exotherm was observed upon addition and the mixture turned light purple. After stirring at ambient temperature for 20 hours, the remaining starting material was consumed by adding boron tribromide (2.5 mL, 1M solution in dichloromethane). The reaction was quenched with aqueous hydrochloric acid (1N, 20 mL) to afford a homogeneous mixture. The layers were separated and the aqueous layer washed with dichloromethane (20 mL). The pH of the aqueous layer was adjusted to 12 by addition of aqueous sodium hydroxide (50%) at which time a solid precipitated out of solution. The solid was stirred for 18 hours, collected by filtration and washed with water. The crude product was purified by chromatography over silica gel (eluting with CMA) to afford a white powder. The powder was triturated with methanol (20 mL). The resulting solid was isolated by filtration, washed with methanol and dried for 4 hours at 65° C. to provide 150 mg of {4-amino-1-[2,2-dimethyl-3-(methylsulfonyl)propyl]-1H-imidazo[4,5-c]quinolin-2-yl}methanol as a white powder, mp 230-232° C.

Anal. Calcd for C₁₇H₂₂N₄O₃S: % C, 56.33; % H, 6.12; % N, 15.46. Found: % C, 56.33; % H, 6.31; % N, 15.27.

Example 365
N-{2-[4-amino-2-(hydroxymethyl)-1H-imidazo[4,5-c]quinolin-1-yl]ethyl}-N′-isopropylurea

A stirring solution of N-{2-[4-amino-2-(ethoxymethyl)-1H-imidazo[4,5-c]quinolin-1-yl]ethyl}-N′-isopropylurea (400 mg, 1.1 mmol) in dichloromethane (50 mL) was sealed with a septum and purged with nitrogen gas. The solution was cooled in an ice/water bath and a 1.0 M solution of boron tribromide in dichloromethane (2.2 mL) was added via syringe. The resulting mixture was stirred for 2 hours while warming to ambient temperature. The mixture was cooled back to 0° C. in an ice/water bath and the second portion of boron tribromide (1.0 M, 5.5 mL) was added. The reaction was stirred for 18 hours while warming to ambient temperature. Aqueous hydrochloric acid (6N, 10 ml) was added and the mixture was stirred for 1 hour. The layers were separated and the aqueous fraction was neutralized by the slow addition of solid sodium hydroxide until the pH reached 14. A fine precipitate formed. The aqueous mixture was extracted with chloroform (2×50 mL) and filtered. The resulting solid (filter cake) was combined with the organic extracts, methanol (50 mL), and silica gel (5 g). The mixture was concentrated under reduced pressure. The crude product absorbed on silica was purified by chromatography using a HORIZON HPFC system (silica cartridge, eluting with 0-35% CMA in chloroform over 2.6 L) followed by recrystallization from acetonitrile to provide 170 mg of N-{2-[4-amino-2-(hydroxymethyl)-1H-imidazo[4,5-c]quinolin-1-yl]ethyl}-N-isopropylurea as an off-white solid, mp>240° C.

¹H NMR (500 MHz, DMSO-d₆) δ 8.30 (d, J=7.9 Hz, 1H), 7.61 (dd, J=8.3, 0.9 Hz, 1H), 7.43 (m, 1H), 7.24 (m, 1H), 6.53 (br s, 2H), 5.99 (t, J=5.8 Hz, 1H), 5.82 (d, J=7.8 Hz, 1H), 5.67 (d, J=5.8 Hz, 1H), 4.75 (d, J=5.8 Hz, 2H), 4.66 (t, J=6.7 Hz, 2H), 3.69 (m, 1H), 3.48 (q, J=6.4 Hz, 2H), 1.01 (d, J=6.5 Hz, 6H);

MS (APCI) m/z 343 (M+H)⁺;

Anal. Calcd. for C₁₇H₂₂N₆O₂: % C, 59.63; % H, 6.48; % N, 24.54. Found: % C, 59.64; % H, 6.59; % N, 24.58.

Example 366
N-{4-[4-amino-2-(hydroxymethyl)-1H-imidazo[4,5-c]quinolin-1-yl]butyl}cyclopentanecarboxamide

Boron tribromide (2.5 equivalents, 14.6 mL of 1 M solution in dichloromethane) was added dropwise to a cooled (ice bath) suspension of N-{4-[4-amino-2-(ethoxymethyl)-1H-imidazo[4,5-c]quinolin-1-yl]butyl}cyclopentanecarboxamide (2.4 g, 5.8 mmol) in dichloromethane (25 mL). The reaction mixture was allowed to slowly warm to ambient temperature and then stirred for 6 days. Additional boron tribromide (5 equivalents, 29 mmol, 29 mL) was added and the reaction was stirred at ambient until starting material was consumed. The reaction was quenched slowly with methanol (100 mL) and then concentrated under reduced pressure. The residue was combined with 6 M hydrochloric acid (100 mL), heated to 50° C., and stirred for 2 hours. The resulting solution was cooled (ice bath) and then free-based (pH 9) with the addition of 6 M aqueous sodium hydroxide. A brown gummy solid formed in the basic aqueous solution. The aqueous liquid was decanted from the solid and acetonitrile was added (30 mL). A white precipitate formed and was isolated by filtration. The white precipitate was then triturated with hot acetonitrile, allowed to cool, isolated by filtration, washed with ether, and dried under vacuum to provide N-{4-[4-amino-2-(hydroxymethyl)-1H-imidazo[4,5-c]quinolin-1-yl]butyl}cyclopentanecarboxamide (0.48 g) as a fine white solid, mp 183-186° C.; MS (ESI) m/z 382 (M+H)⁺; Anal. Calcd for C₂₁H₂₇N₅O₂: C, 65.35; H, 7.18; N, 18.14. Found C, 65.06; H, 6.90; N, 18.13.

Example 367
N-[4-(4-amino-2-hydroxymethyl-1H-imidazo[4,5-c]quinolin-1-yl)butyl]isobutyramide

Boron tribromide (2.5 equivalents, 15.6 mL of 1 M solution in dichloromethane) was added dropwise to a cooled (ice bath) suspension of N-[4-(4-amino-2-ethoxymethyl-1H-imidazo[4,5-c]quinolin-1-yl)butyl]isobutyramide (2.4 g, 6.2 mmol) in dichloromethane (25 mL). The reaction mixture was allowed to slowly warm to ambient temperature and then stirred for 1 day. Additional boron tribromide (5 equivalents, 31 mmol, 31 mL) was added to the mixture. The reaction was quenched slowly with methanol (100 mL) and then concentrated under reduced pressure. The residue was combined with 6 M hydrochloric acid (100 mL), heated to 5° C., and stirred for 2 hours. The resulting solution was cooled (ice bath) and then free-based (pH 9) with the addition of 6 M sodium hydroxide. A brown gummy solid formed in the basic aqueous solution. The resulting solid was extracted with dichloromethane (6×50 mL). The combined extracts were washed with brine (100 mL), dried with magnesium sulfate, filtered, and then concentrated under reduced pressure. This material was purified by prep HPLC (Analogix Separation System, Biotage Si 40+M column, eluted with a gradient of 0-20% methanol in dichloromethane with 1% ammonium hydroxide) to provide a light brown solid. The solid was triturated with hot acetonitrile, allowed to cool, isolated by filtration, washed with ether, and dried under vacuum to provide N-[4-(4-amino-2-hydroxymethyl-1H-imidazo[4,5-c]quinolin-1-yl)butyl]isobutyramide (0.049 g) as a white solid, mp 222-224° C.; MS (ESI) m/z 356 (M+H)⁺; Anal. Calcd for C₁₉H₂₅N₅O₂.0.25HBr.0.10H₂O: C, 60.46; H, 6.80; N, 18.55. Found C, 60.26; H, 6.64; N, 18.43.

Example 368
N-[4-(4-amino-2-hydroxymethyl-1H-imidazo[4,5-c]quinolin-1-yl)butyl]methanesulfonamide

Boron tribromide (2.5 equivalents, 20 mL of 1 M solution in dichloromethane) was added dropwise to a cooled (ice bath) suspension of N-[4-(4-amino-2-ethoxymethyl-1H-imidazo[4,5-c]quinolin-1-yl)butyl]methanesulfonamide (3 g, 7.92 mmol) in dichloromethane (20 mL). The reaction mixture was allowed to slowly warm to ambient temperature and then stirred for 4 hours. Additional boron tribromide (2 mL) was added and the mixture was stirred for 3 hours. The reaction was quenched slowly with methanol (20 mL) and then concentrated under reduced pressure. The residue was combined with 6 M hydrochloric acid (50 mL), heated to 50° C., and stirred for 2 hours. The resulting solution was concentrated under reduced pressure to a slurry that cooled (ice bath) and then free-based with the addition of 7 M ammonia in methanol (40 mL). The mixture was concentrated under reduced pressure and the addition of 7 M ammonia in methanol (40 mL) was repeated 2 more times. The concentrated brown sludge like material was purified by prep HPLC (ISCO Combiflash Separation System, Biotage Si 40+M column, eluted with a gradient of methanol in dichloromethane with 1% ammonium hydroxide) to provide a light brown solid. The solid was triturated with hot acetonitrile, allowed to cool, isolated by filtration, washed with ether, and dried under vacuum to provide N-[4-(4-amino-2-hydroxymethyl-1H-imidazo[4,5-c]quinolin-1-yl)butyl]methanesulfonamide (0.1 g) as a fine beige solid, mp 216-219° C.; MS (ESI) m/z 364 (M+H)⁺; Anal. Calcd for C₁₆H₂₁N₅O₃S: C, 52.88; H, 5.82; N, 19.27. Found C, 52.62; H, 5.71; N, 19.02.

Example 369
(4-Amino-1-{4-[(methylsulfonyl)amino]butyl}-1H-imidazo[4,5-c]quinolin-2-yl)methyl N-[(benzyloxy)carbonyl]-L-valinate

To a stirred suspension of N-[4-(4-amino-2-hydroxymethyl-1H-imidazo[4,5-c]quinolin-1-yl)butyl]methanesulfonamide (2.1 g, 5.8 mmol) in THF was added triphenylphosphine (1.5 equivalents, 8.7 mmol, 2.2 g) followed by CBZ-L-valine (1.5 equivalents, 8.7 mmol, 2.3 g). The suspension was stirred for 5 min after which it was cooled in an ice-bath. To this cooled reaction mixture diisopropyl azodicarboxylate (DIAD, 1.8 equivalents, 10.4 mmol, 2.0 mL) was added and the reaction was warmed to room temperature and stirred overnight. The solvent was evaporated under reduced pressure and the crude solid was purified by prep HPLC (ISCO Combiflash Separation System, Biotage Si 40+M column, eluted with a gradient of 0-8% methanol in dichloromethane with 1% ammonium hydroxide) to provide a solid. The solid was heated in diethyl ether and filtered to afford (4-amino-1-{4-[(methylsulfonyl)amino]butyl}-1H-imidazo[4,5-c]quinolin-2-yl)methyl N-[(benzyloxy)carbonyl]-L-valinate (2 g) as a beige solid, mp 99-100° C.; MS (ESI) m/z 597 (M+H)⁺; Anal. Calcd for C₂₉H₃₆N₆O₆S: C, 58.37; H, 6.08; N, 14.08. Found C, 57.98; H, 6.31; N, 13.82.

Example 370
(4-Amino-1-{4-[(methylsulfonyl)amino]butyl}-1H-imidazo[4,5-c]quinolin-2-yl)methyl L-valinate

To a hydrogenation bottle was added (4-amino-1-{4-[(methylsulfonyl)amino]butyl}-1H-imidazo[4,5-c]quinolin-2-yl)methyl N-[(benzyloxy)carbonyl]-L-valinate (1.5 g, 2.5 mmol) followed by a mixture of methanol (30 mL), THF (15 mL) and water (5 mL) and conc HCl (5 mL). To this was added Pd/C (90 mg) and the reaction was hydrogenated at 40 psi (2.8×10⁵Pa) overnight. To the reaction mixture was added conc. HCl (5 mL) and Pd/C (90 mg) and the reaction was hydrogenated at 40 psi (2.8×10⁵Pa) for 18 hours. The reaction was filtered through CELITE filter aid and the filtrate was evaporated to afford a clear oil. The product was isolated by prep HPLC (ISCO Combiflash Separation System, Biotage Si 40+M column, eluted with a gradient of 0-8% methanol in dichloromethane with 1% ammonium hydroxide) to provide (4-amino-1-{4-[(methylsulfonyl)amino]butyl}-1H-imidazo[4,5-c]quinolin-2-yl)methyl L-valinate (0.495 g) as an off white solid, mp 161-163° C.; MS (ESI) m/z 463 (M+H)⁺; Anal. Calcd for C₂₁H₃₀N₆O₄S: C, 54.53; H, 6.54; N, 18.17. Found C, 53.96; H, 6.62; N, 17.85, delta C=0.57.

Example 371
[4-Amino-1-(tetrahydro-2H-pyran-4-ylmethyl)-1H-imidazo[4,5-c]quinolin-2-yl]methanol

Part A

Under a nitrogen atmosphere THF (90 mL) and triethylamine (17.5 mL, 125.6 mmol) were added sequentially to a mixture of crude 4-chloro-3-nitroquinoline (13.10 g, 62.81 mmol) and 1-tetrahydro-2H-pyran-4-ylmethylamine hydrochloride (10.0 g, 65.95 mmol). The reaction mixture was placed in an oil bath at 45° C. for 1 hour and then concentrated under reduced pressure. The residue was diluted with THF (30 mL) and water (200 mL). The THF was removed under reduced pressure. A solid was isolated by filtration and dried to provide 16.10 g of 3-nitro-N-(tetrahydro-2H-pyran-4-ylmethyl)quinolin-4-amine as a light yellow solid.

Part B

A mixture of 3-nitro-N-(tetrahydro-2H-pyran-4-ylmethyl)quinolin-4-amine (2.50 g), 10% palladium on carbon (0.25 g), and ethanol (40 mL) was placed under hydrogen pressure on a Parr apparatus. When the reaction was complete, the mixture was filtered through a layer of CELITE filter agent. The filter cake was washed with ethanol. The filtrate was concentrated under reduced pressure to provide 2.23 g of N⁴-(tetrahydro-2H-pyran-4-ylmethyl)quinoline-3,4-diamine as a yellowish-orange oil.

Part C

Chloroacetyl chloride (12 mL, 151 mmol) was dissolved in dichloromethane (30 mL) and added via addition funnel, over 20 minutes, to a stirring solution of N4-(tetrahydro-2H-pyran-4-ylmethyl)quinoline-3,4-diamine (35.3 g, 137 mmol) in dichloromethane (300 mL). The resulting solution was stirred at ambient temperature under nitrogen for 24 hours at which point the solution was heated to 40° C. for an additional 24 hours. The mixture was cooled to ambient temperature, diluted with dichloromethane (150 mL) and transferred to a separatory funnel. The organic layer was washed with water (2×200 mL) and brine (2×200 mL), dried over magnesium sulfate, filtered and concentrated under reduced pressure to provide 38.3 g of 2-(chloromethyl)-1-(tetrahydro-2H-pyran-4-ylmethyl)-1H-imidazo[4,5-c]quinoline as a light brown solid.

Part D

3-Chloroperoxybenzoic acid (mCPBA) (3.8 g of 77% pure material, 14.2 mmol) was added to a stirring solution of 2-(chloromethyl)-1-(tetrahydro-2H-pyran-4-ylmethyl)-1H-imidazo[4,5-c]quinoline (3.0 g, 9.50 mmol) in dichloromethane (60 mL). After 15.5 hours, ammonium hydroxide (12 mL) and then p-toluenesulfonyl chloride (2.2 g, 11.4 mmol) were added to the stirring solution and the biphasic mixture was stirred at ambient temperature for 3 hours. The reaction was diluted with water (50 mL) and then transferred to a separatory funnel. The aqueous layer was extracted with dichloromethane (3×100 mL) and the combined organic fractions dried over magnesium sulfate, filtered and concentrated under reduced pressure. The residue was purified by column chromatography using a HORIZON HPFC system (silica cartridge, eluting with 3-20% methanol in dichloromethane) to provide 1.6 g of 2-(chloromethyl)-1-(tetrahydro-2H-pyran-4-ylmethyl)-1H-imidazo[4,5-c]quinolin-4-amine as a yellow solid.

Part E

Potassium acetate (0.41 g, 4.16 mmol) and potassium iodide (0.28 g, 1.66 mmol) were added to a stirring solution of 2-(chloromethyl)-1-(tetrahydro-2H-pyran-4-ylmethyl)-1H-imidazo[4,5-c]quinolin-4-amine (0.55 g, 1.66 mmol) and the resulting suspension was heated to 50° C. After 17 hours, the suspension was cooled to ambient temperature and concentrated under reduced pressure. The residue was suspended in methanol (10 mL) and water (5 mL) and lithium hydroxide monohydrate (0.35 g, 8.31 mmol) was added in one portion. The resulting solution was stirred at ambient temperature 18 hours and concentrated under reduced pressure. The residue was diluted with water (20 mL) and neutralized with hydrochloric acid (6 N in water). The aqueous layer was extracted with dichloromethane (2×50 mL) and ethyl acetate (50 mL). The combined organic fractions were concentrated to a yellow solid which was crystallized from acetonitrile. The crystals were isolated by filtration and dried in a vacuum oven at 65° C. to provide 0.20 g of [4-amino-1-(tetrahydro-2H-pyran-4-ylmethyl)-1H-imidazo[4,5-c]quinolin-2-yl]methanol as an off-white solid, mp 239-241° C.

Anal. calcd for C₁₇H₂₀N₄O₂.0.2H₂O: C, 64.62; H, 6.51; N, 17.73. Found: C, 64.45; H, 6.69; N, 17.62.

Examples 372-450
Part A

A solution of 1-(4-aminobutyl)-2-methoxymethyl-1H-imidazo[4,5-c]quinoline-4-amine (30 mg, 1 eq, prepared according to the general method of Example 3 using methoxyacetyl chloride in lieu of 3-methoxypropionyl chloride) and N,N-diisopropylethylamine (2 eq) in N,N-dimethylacetamide (1 mL) was added to a tube containing a reagent (1.1 eq) from the table below. The reaction mixture was vortexed overnight and then quenched with water (100 μL). The solvents were removed by vacuum centrifugation. The residue was purified by solid-supported liquid-liquid extraction according to the following procedure. The sample was dissolved in chloroform (1 mL) then loaded onto diatomaceous earth that had been equilibrated with 1 M sodium hydroxide (600 μL) for about 20 minutes. After 10 minutes chloroform (500 μL) was added to elute the product from the diatomaceous earth into a well of a collection plate. After an additional 10 minutes the process was repeated with additional chloroform (500 μL). The solvent was then removed by vacuum centrifugation.

Part B

The residue (in a test tube) was combined with dichloromethane (500 μL) and the tube was vortexed to dissolve the solids. The solution was cooled (0° C.) and then combined with boron tribromide (400 μL of 1 M in dichloromethane). The mixture was vortexed for 5 minutes, chilled for 30 minutes, and then vortexed at ambient temperature for 64 hours. Additional dichloromethane (500 μL) and boron tribromide (400 μL of 1 M in dichloromethane) were added and the mixture was vortexed overnight. The solvent was then removed by vacuum centrifugation. The residue was diluted with methanol (500 μL) and hydrochloric acid (500 μL of 6 N). The solvents were removed by vacuum centrifugation. The compounds were purified according to the method described in Examples 8-72. The table below shows the reagent used for each example, the structure of the resulting compound, and the observed accurate mass for the isolated trifluoroacetate salt.

Measured Mass

Example
Reagent
R
(M + H)

372
None

286.1658

373
Cyclopropanecarbonylchloride

354.1907

374
Methoxyacetyl chloride

344.1699

375
Cyclobutanecarbonylchloride

368.2050

376
Isovaleryl chloride

370.2206

377
Pentanoyl chloride

370.2208

378
Benzoyl chloride

390.1909

379
Cyclohexanecarbonylchloride

396.2412

380
Cyclopentylacetylchloride

396.2411

381
m-Toluoyl chloride

404.2069

382
o-Toluoyl chloride

404.2072

383
p-Toluoyl chloride

404.2108

384
Phenylacetyl chloride

404.2056

385
Dimethylaminoacetylchloride hydrochloride

371.2157

386
2-Fluorobenzoyl chloride

408.1819

387
3-Fluorobenzoyl chloride

408.1811

388
4-Fluorobenzoyl chloride

408.1819

389
3-Cyanobenzoyl chloride

415.1847

390
Hydrocinnamoyl chloride

418.2200

391
2-Methoxybenzoylchloride

406.1880

392
3-Methoxybenzoylchloride

406.1876

293
p-Anisoyl chloride

406.1860

394
3-Chlorobenzoyl chloride

424.1517

395
4-Chlorobenzoyl chloride

424.1525

396
Isonicotinoyl chloridehydrochloride

391.1874

397
Nicotinoyl chloridehydrochloride

391.1895

398
Picolinoyl chloridehydrochloride

391.1846

399
trans-2-Phenyl-1-cyclopropanecarbonylchloride

430.2213

400
Methanesulfonyl chloride

364.1421

401
Ethanesulfonyl chloride

378.1595

402
1-Propanesulfonylchloride

392.1753

403
Dimethylsulfamoylchloride

393.1685

404
1-Butanesulfonyl chloride

406.1881

405
Benzenesulfonyl chloride

426.1591

406
1-Methylimidazole-4-sulfonyl chloride

430.1668

407
2-Thiophenesulfonylchloride

432.1135

408
3-Methylbenzenesulfonylchloride

440.1728

409
o-Toluenesulfonylchloride

440.1758

410
p-Toluenesulfonylchloride

440.1766

411
2-Fluorobenzenesulfonylchloride

444.1479

412
3-Fluorobenzenesulfonylchloride

444.1517

413
4-Fluorobenzenesulfonylchloride

444.1496

414
3-Cyanobenzenesulfonylchloride

451.1568

415
4-Cyanobenzenesulfonylchloride

451.1579

416
beta-Styrenesulfonylchloride

452.1725

417
3-Methoxybenzenesulfonylchloride

442.1534

418
4-Methoxybenzenesulfonylchloride

442.1557

419
2-Chlorobenzenesulfonylchloride

460.1173

420
3-Chlorobenzenesulfonylchloride

460.1242

421
4-Chlorobenzenesulfonylchloride

460.1191

422
3-Pyridinesulfonylchloride hydrochloride

427.1530

423
3,4-Dimethoxybenzenesul-fonyl chloride

458.1452

424
3,4-Dichloro-benzenesulfonylchloride

494.0806

425
Methyl isocyanate

343.1862

426
Ethyl isocyanate

357.2018

427
Isopropyl isocyanate

371.2181

428
n-Propyl isocyanate

371.2187

429
n-Butyl isocyanate

385.2314

430
Cyclopentyl isocyanate

397.2312

431
Pentyl isocyanate

399.2512

432
Phenyl isocyanate

405.2047

433
Cyclohexyl isocyanate

411.2473

434
2-Fluorophenylisocyanate

423.1959

435
3-Fluorophenylisocyanate

423.1924

436
4-Cyanophenylisocyanate

430.1979

437
(R)-(+)-alpha-Methylbenzyl isocyanate

433.2370

438
(S)-(−)-alpha-Methylbenzyl isocyanate

433.2327

439
2-Phenylethylisocyanate

433.2333

440
2-Methoxyphenylisocyanate

421.2006

441
4-Methoxyphenylisocyanate

421.1958

442
2-Chlorophenylisocyanate

439.1650

443
4-Chlorophenylisocyanate

439.1656

444
trans-2-Phenylcyclopropylisocyanate

445.2328

445
N,N-Dimethylcarbamoylchloride

357.2005

446
1-Pyrrolidinecarbonylchloride

383.2168

447
1-Piperidinecarbonylchloride

397.2329

448
4-Morpholinylcarbonylchloride

399.2112

449
4-Methyl-1-Piperazinecarbonylchloride

412.2439

450
N-Methyl-N-phenylcarbamoyl chloride

419.2167

Examples 451-466
Part A

A solution of 1-(2-amino-2-methylpropyl)-2-methoxymethyl-1H-imidazo[4,5-c]quinoline-4-amine (31 mg, 1 eq, prepared according to the general method of Example 3 using methoxyacetyl chloride in lieu of 3-methoxypropionyl chloride and tert-butyl N-{2-[(3-aminoquinolin-4-yl)amino]-1,1-dimethylethyl}carbamate in lieu of tert-butyl N-{4-[(3-aminoquinolin-4-yl)amino]butyl}carbamate) and N,N-diisopropylethylamine (2 eq) in N,N-dimethylacetamide (1 mL) was placed in a test tube. A reagent (1.1 eq) from the table below was added and the reaction mixture was vortexed overnight. The reaction was quenched with concentrated ammonium hydroxide (100 μL) and the solvents were removed by vacuum centrifugation.

Part B

The residue (in a test tube) was combined with dichloromethane (1 mL) and the tube was vortexed to dissolve the solids. The solution was cooled (0° C.) and then combined with boron tribromide (400 μL of 1 M in dichloromethane). The reaction was maintained at about 0° C. for 20 minutes. Methanol (1 mL) and hydrochloric acid (500 μL of 6 N) were added and the tube was vortexed for about 30 minutes. The solvents were removed by vacuum centrifugation. The compounds were purified according to the method described in Examples 8-72. The table below shows the reagent used for each example, the structure of the resulting compound, and the observed accurate mass for the isolated trifluoroacetate salt.

Measured Mass

Example
Reagent
R
(M + H)

451
None

286.1687

452
Cyclopropanecarbonylchloride

354.1936

453
Butyryl chloride

356.2094

454
Isobutyryl chloride

356.2119

455
Cyclopentanecarbonylchloride

382.2259

456
Benzoyl chloride

390.1908

457
Nicotinoyl chloridehydrochloride

391.1844

458
Methanesulfonylchloride

364.1414

459
Benzenesulfonylchloride

426.1617

460
2,2,2-Trifluoroethane-sulfonyl chloride

432.1339

461
3-Fluorobenzenesul-fonyl chloride

444.1523

462
n-Propyl isocyanate

371.2215

463
Cyclopentylisocyanate

397.2327

464
Phenyl isocyanate

405.2063

465
Cyclohexyl isocyanate

411.2515

466
3-Fluorophenylisocyanate

423.1955

Examples 467-478
Part A

To a round-bottomed flask containing 1-(4-aminobutyl)-2-methoxymethyl-1H-imidazo[4,5-c]quinolin-4-amine (10.0 g, 33.4 mmol) was added methanol (160 mL) followed by acetic acid (40 mL). The reaction was stirred for 5 minutes and pyridine 3-carboxaldehyde (5.4 g, 50.1 mmol) was added and the reaction was stirred overnight at ambient temperature. Sodium cyanoborohydride (1 M in THF, 33.4 mL, 33.4 mmol) was added to the resultant imine in portions over 10 minutes. After 45 minutes the solvent was evaporated to afford an oil. To the oil was added saturated aqueous sodium bicarbonate (200 mL) and the aqueous layer was washed with ethyl acetate (200 mL) and dichloromethane (200 mL). The product was extracted from the aqueous with 20% methanol (2×100 mL) in dichloromethane. The organic layers were combined and the solvent evaporated to afford crude 2-methoxymethyl-1-{4-[(pyridin-3-ylmethyl)amino]butyl}-1H-imidazo[4,5-c]quinolin-4-amine (about 2 g). The aqueous layer was again extracted with 20% dimethylformamide (2×100 mL) in dichloromethane. The organic layers were combined and the solvent evaporated to afford crude 2-methoxymethyl-1-{4-[(pyridin-3-ylmethyl)amino]butyl}-1H-imidazo[4,5-c]quinolin-4-amine (about 2 g).

Part B

A solution of 2-methoxymethyl-1-{4-[(pyridin-3-ylmethyl)amino]butyl}-1H-imidazo[4,5-c]quinolin-4-amine (40 mg, 1 eq) and N,N-diisopropylethylamine (2 eq) in N,N-dimethylacetamide (1 mL) was added to a tube containing a reagent (1.1 eq) from the table below. The reaction mixture was vortexed for 4 hours and then quenched with water (50 μL). The solvents were removed by vacuum centrifugation. The residue was purified by solid-supported liquid-liquid extraction according to the following procedure. The sample was dissolved in chloroform (1 mL) then loaded onto diatomaceous earth that had been equilibrated with 1 M sodium hydroxide (600 μL) for about 20 minutes. After 10 minutes chloroform (500 μL) was added to elute the product from the diatomaceous earth into a well of a collection plate. After an additional 10 minutes the process was repeated with additional chloroform (500 μL). The solvent was then removed by vacuum centrifugation.

Part C

The residue (in a test tube) was combined with dichloromethane (500 μL) and the tube was vortexed to dissolve the solids. The solution was cooled (0° C.) and then combined with boron tribromide (400 μL of 1 M in dichloromethane). The mixture was vortexed for 10 minutes, chilled for 30 minutes, and then vortexed at ambient temperature overnight. The solvent was then removed by vacuum centrifugation. The residue was diluted with methanol (500 μL) and hydrochloric acid (500 μL of 6 N) and the mixture was vortexed for about 30 minutes. The solvents were removed by vacuum centrifugation. The compounds were purified according to the method described in Examples 8-72. The table below shows the reagent used for each example, the structure of the resulting compound, and the observed accurate mass for the isolated trifluoroacetate salt.

Measured

Exam-

Mass

ple
Reagent
R
(M + H)

467
None

377.2087

468
Isobutyryl chloride

447.2468

469
Cyclohexanecarbonylchloride

487.2783

470
Phenylacetyl chloride

495.2465

471
4-Fluorobenzoylchloride

499.2272

472
3-Methoxybenzoylchloride

497.2263

473
1-Methylimidazole-4-sulfonyl chloride

521.2071

474
2,2,2-Trifluoroethanesulfonylchloride

523.1717

475
alpha-Toluenesulfonylchloride

531.2134

476
3-Methoxybenzene-sulfonyl chloride

533.1941

477
Isopropyl isocyanate

462.2611

478
3-Fluorophenylisocyanate

514.2357

Examples 479-543

The compounds in the table below were prepared and purified according to the methods of Parts B and C of Examples 467-478 using 1-(4-benzylaminobutyl)-2-ethoxymethyl-1H-imidazo[4,5-c]quinolin-4-amine in lieu of 2-methoxymethyl-1-{4-[(pyridin-3-ylmethyl)amino]butyl}-1H-imidazo[4,5-c]quinolin-4-amine. 1-(4-Benzylaminobutyl)-2-ethoxymethyl-1H-imidazo[4,5-c]quinolin-4-amine was prepared according to the general method of Part A of Examples 467-478 using benzaldehyde in lieu of pyridine 3-carboxaldehyde and 1-(4-aminobutyl)-2-ethoxymethyl-1H-imidazo[4,5-c]quinolin-4-amine in lieu of 1-(4-aminobutyl)-2-methoxymethyl-1H-imidazo[4,5-c]quinolin-4-amine. The table below shows the reagent used for each example, the structure of the resulting compound, and the observed accurate mass for the isolated trifluoroacetate salt.

Measured Mass

Example
Reagent
R
(M + H)

479
Cyclobutanecarbonylchloride

458.2550

480
DL-2-Methylbutyrylchloride

460.2707

481
Isovaleryl chloride

460.2714

482
Pentanoyl chloride

460.2730

483
Pivaloyl chloride

460.2714

484
Cyclopentanecarbonylchloride

472.2712

485
tert-Butylacetyl chloride

474.2879

486
Benzoyl chloride

480.2398

487
Thiophene-2-carbonylchloride

486.1971

488
Cyclohexanecarbonylchloride

486.2893

489
Cyclopentylacetylchloride

486.2818

490
m-Toluoyl chloride

494.2577

491
o-Toluoyl chloride

494.2531

492
p-Toluoyl chloride

494.2527

493
3-Fluorobenzoyl chloride

498.2307

494
4-Fluorobenzoyl chloride

498.2326

495
3-Cyanobenzoyl chloride

505.2378

496
4-Cyanobenzoyl chloride

505.2387

497
Hydrocinnamoyl chloride

508.2715

498
2-Methoxybenzoylchloride

496.2311

499
3-Methoxybenzoylchloride

496.2314

500
p-Anisoyl chloride

496.2365

501
3-Chlorobenzoyl chloride

514.2026

502
4-Chlorobenzoyl chloride

514.2041

503
Picolinoyl chloridehydrochloride

481.2361

504
trans-2-Phenyl-1-cyclopropanecarbonylchloride

520.2695

505
4-Dimethylaminobenzoylchloride

523.2802

506
1-Propanesulfonylchloride

482.2232

507
Dimethylsulfamoylchloride

483.2196

508
2-Thiophenesulfonylchloride

522.1613

509
alpha-Toluenesulfonylchloride

530.2239

510
o-Toluenesulfonylchloride

530.2197

511
4-Fluorobenzenesulfonylchloride

534.2028

512
3,5-Dimethylisoxazole-4-sulfonyl chloride

535.2106

513
2-Cyanobenzenesulfonylchloride

541.1968

514
3-Cyanobenzenesulfonylchloride

541.2035

515
beta-Styrene sulfonylchloride

542.2234

516
3-Methoxybenzenesulfonylchloride

532.2052

517
4-Methoxybenzenesulfonylchloride

532.2037

518
3-Pyridine sulfonylchloride hydrochloride

517.2015

519
2,5-Dimethoxybenzene-sulfonyl chloride

548.1964

520
2,3-Dichloro-benzenesulfonylchloride

584.1294

521
3,5-Dichlorobenzene-sulfonyl chloride

584.1282

522
Methyl isocyanate

433.2361

523
Ethyl isocyanate

447.2538

524
Isopropyl isocyanate

461.2663

525
n-Propyl isocyanate

461.2691

526
n-Butyl isocyanate

475.2860

527
sec-Butyl isocyanate

475.2849

528
Pentyl isocyanate

489.3005

529
Phenyl isocyanate

495.2511

530
Cyclohexyl isocyanate

501.2978

531
Benzyl isocyanate

509.2675

532
3-Fluorophenylisocyanate

513.2467

533
4-Fluorophenylisocyanate

513.2388

534
Cycloheptyl isocyanate

515.3081

535
Cyclohexanemethylisocyanate

515.3163

536
4-Cyanophenylisocyanate

520.2483

537
3,4-Dimethylphenylisocyanate

523.2786

538
(S)-(−)-alpha-Methylbenzyl isocyanate

523.2786

539
2-Methylbenzylisocyanate

523.2860

540
N,N-Dimethylcarbamoylchloride

447.2511

541
Diethylcarbamyl chloride

475.2828

542
1-Piperidinecarbonylchloride

487.2839

543
N-(4-Chlorobutyl)-N-methylcarbamyl chloride

523.2588

Examples 544-550

The compounds in the table below were prepared according to the general method of Examples 111-140. The table shows a reference for the ether starting material, the structure of the resulting compound, and the observed accurate mass for the isolated trifluoroacetate salt.

Measured

Reference

Mass

Example
(ether)
R₁
R₂
(M + H)

544
U.S. Pat. No.6,667,312*

335.1158

545
U.S. Pat. No.6,677,349*

336.1098

546
U.S. Pat. No.6,677,349*

364.1454

547
U.S. Pat. No.6,677,347Example 57

380.1391

548
U.S. Pat. No.6,756,382*

444.0999

549
U.S. Pat. No.6,683,088Example 1

394.1588

550
U.S. Pat. No.6,677,349Example 242

496.2401

*Although not specifically exemplified, the compound is readily prepared using the disclosed synthetic methods.

Example 551
[4-Amino-1-(2-fluoro-2-methylpropyl)-1H-imidazo[4,5-c][1,5]naphthyridin-2-yl]methanol

Part A

A solution of 1-amino-2-methylpropan-2-ol (23.4 g, 263 mmol) dissolved in 150 mL of THF was treated with 150 mL of 1.8 M aqueous NaOH solution and the mixture was placed in an ice bath. A solution of di-tert-butyl dicarbonate (57.3 g, 263 mmol) in 150 mL THF was then added drop-wise over 45 min. The mixture was allowed to warm to ambient temperature overnight. The THF was removed under reduced pressure and the remaining aqueous solution was treated with 1 M H₂SO₄until the pH reached 3. The mixture was extracted with 200 mL of EtOAc. The organic portion was washed with H₂O and brine, dried over Na₂SO₄, and concentrated under reduced pressure give tert-butyl 2-hydroxy-2-methylpropylcarbamate (50.4 g) as a colorless syrup which solidified on standing.

Part B

A stirred solution of tert-butyl 2-hydroxy-2-methylpropylcarbamate (7.81 g, 41.3 mmol) dissolved in 300 mL of anhydrous CH₂Cl₂was cooled to −78° C. under an atmosphere of N₂. The reaction mixture was treated with diethylaminosulfur trifloride (DAST) (6.2 mL, 47 mmol) and allowed to warm to ambient temperature overnight. The reaction mixture was treated with saturated NaHCO₃solution and the layers were separated. The organic portion was washed successively with saturated NaHCO₃solution, H₂O and brine. The organic portion was dried over Na₂SO₄, filtered and concentrated under reduced pressure. Chromatography (SiO₂, 10% EtOAc/hexanes) gave 6.27 g of tert-butyl 2-fluoro-2-methylpropylcarbamate as an amber oil which solidified on standing.

Part C

tert-Butyl 2-fluoro-2-methylpropylcarbamate (6.27 g, 32.8 mmol) was treated with 45 mL of 3.0 M HCl in ethanol and the mixture was heated to 90° C. for 2 hours. The reaction mixture was then concentrated under reduced pressure to give 4.02 g of 2-fluoro-2-methylpropan-1-amine hydrochloride as a white solid.

Part D

2-Fluoro-2-methylpropan-1-amine hydrochloride (4.02 g, 31.4 mmol) was dissolved in 80 mL of dry CH₂Cl₂. Triethylamine (13.1 mL, 94.2 mmol) and 4-chloro-3-nitro[1,5]naphthyridine (6.56 g, 31.4 mmol) were then added and the reaction was stirred under N₂for 2 days. The reaction mixture was then concentrated under reduced pressure to give a dark-yellow solid. The solid was treated with 200 mL of H₂O and the mixture was heated to reflux with rapid stirring. The mixture was cooled and the yellow solid was isolated by filtration. The material was washed with H₂O and the dried under vacuum to give N-(2-fluoro-2-methylpropyl)-3-nitro[1,5]naphthyridin-4-amine (8.36 g) as a yellow powder.

Part E

A solution of N-(2-fluoro-2-methylpropyl)-3-nitro[1,5]naphthyridin-4-amine (2.64 g, 10.0 mmol) dissolved in 80 mL of acetonitrile was placed in a pressure bottle. Platinum on carbon (5%, 500 mg) was then added and the reaction mixture was shaken under H₂at 50 PSI (3.4×10⁵Pa). After 5 hours, the reaction mixture was filtered through a pad of CELITE filter agent. The pad was rinsed with acetonitrile and the combined filtrates were concentrated under reduced pressure to give 2.12 g of N⁴-(2-fluoro-2-methylpropyl)[1,5]naphthyridine-3,4-diamine as a brown foam.

Part F

N⁴-(2-Fluoro-2-methylpropyl)[1,5]naphthyridine-3,4-diamine (2.12 g, 9.06 mmol) was dissolved in 90 mL of anhydrous CH₂Cl₂and the stirred solution was cooled to 0° C. under N₂. Triethylamine (1.39 mL, 10.0 mmol) and acetoxyacetyl chloride (1.07 mL, 10.0 mmol) were then added and the reaction mixture was stirred overnight. The reaction mixture was concentrated under reduced pressure and the resulting material was dissolved in 90 mL of ethanol and treated with 5 mL of triethylamine. The mixture was heated at reflux for 4 days. The reaction mixture was then cooled and concentrated under reduced pressure to give a purple solid. The purple solid was partitioned between CH₂Cl₂(75 mL) and H₂O (75 mL). The layers were separated and the aqueous portion was extracted with CH₂Cl₂(2×20 mL). The combined organic portions were washed with brine, dried over Na₂SO₄, filtered and concentrated under reduced pressure to give a purple solid. The resulting material was dissolved in 50 mL of methanol and treated with 1 mL of saturated aqueous K₂CO₃solution. After 1 hour, the mixture was treated with 3.5% NaH₂PO₄solution and the methanol was removed by evaporation under reduced pressure. A brown solid precipitated out of the aqueous solution and was isolated by filtration. The brown solid was rinsed with H₂O and then dried to give 1.81 g of [1-(2-fluoro-2-methylpropyl)-1H-imidazo[4,5-c][1,5]naphthyridin-2-yl]methanol.

Part G

A solution of [1-(2-fluoro-2-methylpropyl)-1H-imidazo[4,5-c][1,5]naphthyridin-2-yl]methanol (1.53 g, 5.58 mmol) dissolved in 50 mL of CH₂Cl₂was treated with triethylamine (1.55 mL, 11.2 mmol), acetic anhydride (663 μL, 6.70 mmol), and 10 mg of 4-(dimethylamino)pyridine (DMAP). After stirring for 2 hours, the reaction mixture was treated with saturated NaHCO₃solution and the layers were separated. The organic portion was washed successively with 3.5% NaH₂PO₄solution, H₂O and brine. The organic portion was dried over Na₂SO₄, filtered and concentrated under reduced pressure. Chromatography (SiO₂, 40-60% acetone/hexanes) gave 1.59 g of [1-(2-fluoro-2-methylpropyl)-1H-imidazo[4,5-c][1,5]naphthyridin-2-yl]methyl acetate as an off-white powder.

Part H

[1-(2-Fluoro-2-methylpropyl)-1H-imidazo[4,5-c][1,5]naphthyridin-2-yl]methyl acetate (1.59 g, 5.03 mmol) was dissolved in 50 mL of CH₂Cl₂and treated with 3-chloroperoxybenzoic acid (1.52 g, 57-86% purity). After stirring for 2 hours, the reaction mixture was treated with 25 mL of CH₂Cl₂and 20 mL of 5% Na₂CO₃solution and the layers were separated. The organic layer was washed with H₂O (20 mL) and brine (20 mL), dried over Na₂SO₄, filtered, and concentrated under reduced pressure to give 1.67 g of [1-(2-fluoro-2-methylpropyl)-5-oxido-1H-imidazo[4,5-c][1,5]naphthyridin-2-yl]methyl acetate as an off-white solid.

Part I

[1-(2-Fluoro-2-methylpropyl)-5-oxido-1H-imidazo[4,5-c][1,5]naphthyridin-2-yl]methyl acetate (1.67 g, 5.03 mmol) was dissolved in 50 mL of CH₂Cl₂and treated with 5 mL of concentrated aqueous NH₄OH solution. The mixture was stirred rapidly and then p-toluenesulfonyl chloride (1.05 g, 5.53 mmol) was carefully added. Rapid stirring was continued for 1 hour. The reaction mixture was then treated with 20 mL of H₂O. The layers were separated and the organic portion was washed successively with 5% Na₂CO₃solution, H₂O and brine. The organic portion was dried over Na₂SO₄, filtered, and concentrated under reduced pressure. Chromatography (SiO₂, 2.5% methanol/CHCl₃) gave 1.13 g of [4-amino-1-(2-fluoro-2-methylpropyl)-1H-imidazo[4,5-c][1,5]naphthyridin-2-yl]methyl acetate as a light-yellow solid.

Part J

A solution of [4-amino-1-(2-fluoro-2-methylpropyl)-1H-imidazo[4,5-c][1,5]naphthyridin-2-yl]methyl acetate (1.13 g, 3.41 mmol) dissolved in 10 mL of methanol was treated with 10 mL of a 7% solution of ammonia in methanol. The mixture was stirred for 2 hours and then concentrated under reduced pressure. The resulting solid was treated with H₂O and the mixture was heated to reflux for 15 minutes. The mixture was cooled and the resulting light-yellow solid was isolated by filtration. The light-yellow solid was then treated with 20 mL of CH₂Cl₂and the mixture was stirred rapidly for several minutes. The mixture was filtered and the resulting white solid was washed with several portions of cold CH₂Cl₂and dried with suction. Crystallization from ethanol/H₂O gave 477 mg of [4-amino-1-(2-fluoro-2-methylpropyl)-1H-imidazo[4,5-c][1,5]naphthyridin-2-yl]methanol as fluffy cream colored crystals, mp 240-241° C. ¹H NMR (300 MHz, DMSO-d₆) δ 8.50 (dd, J=1.5, 4.3 Hz, 1H), 7.93 (dd, J=1.5, 8.4 Hz, 1H), 7.46 (dd, J=4.3, 8.4 Hz, 1H), 6.92 (s, 2H), 5.62 (t, J=5.8 Hz, 1H), 5.33 (br s, 2H), 4.83 (d, J=4.7 Hz, 2H), 1.33 (d, J=20.3 Hz, 6H); ¹³C NMR (125 MHz, DMSO-d₆) δ 154.3, 152.7, 143.1, 140.8, 134.2, 133.4, 133.2, 128.7, 122.5, 96.8 (d, J=170 Hz), 56.7 (d, J=9.5 Hz), 52.7 (d, J=21.4 Hz), 24.5; MS (ESI) m/z 290 (M+H)⁺; Anal. calcd for C₁₄H₁₆FN₅O: C, 58.12; H, 5.57; N, 24.21. Found: C, 58.19; H, 5.54; N, 24.16.

Example 552
2-[4-Amino-1-(2-fluoro-2-methylpropyl)-1H-imidazo[4,5-c][1,5]naphthyridin-2-yl]ethanol

Part A

N⁴-(2-Fluoro-2-methylpropyl)[1,5]naphthyridine-3,4-diamine (2.34 g, 10.0 mmol) was dissolved in 80 mL of anhydrous CH₂Cl₂and the stirred solution was cooled to 0° C. under N₂. Triethylamine (2.78 mL, 10.0 mmol) and 3-(benzyloxy)propanoyl chloride, prepared by the method of Li, J. Med. Chem., 42, pp. 706-721, (2.13 g, 10.0 mmol), were then added and the reaction mixture was stirred overnight. The reaction mixture was concentrated under reduced pressure. The resulting material was dissolved in 80 mL of ethanol and combined with 5 mL of triethylamine and the mixture was heated to reflux for 4 days. The reaction mixture was then cooled and concentrated under reduced pressure. The resulting solid was partitioned between CH₂Cl₂(75 mL) and H₂O (75 mL). The layers were separated and the organic portion was washed with brine, dried over Na₂SO₄, filtered, and concentrated under reduced pressure to give a solid. Chromatography (SiO₂, 1-2% CMA/CHCl₃) gave 0.83 g of uncyclized amide (3-(benzyloxy)-N-{4-[(2-fluoro-2-methylpropyl)amino][1,5]naphthyridin-3-yl}propanamide) and the desired 2-[2-(benzyloxy)ethyl]-1-(2-fluoro-2-methylpropyl)-1H-imidazo[4,5-c][1,5]naphthyridine. Additional chromatography (10% methanol/CHCl₃) of the desired material gave 1.39 g of a light-orange syrup. The isolated amide was converted to the desired imidazole by dissolving the material in 10 mL of 7% ammonia in methanol. The mixture was placed in a stainless-steel pressure vessel and the vessel was sealed and heated to 150° C. overnight. The reaction mixture was cooled and concentrated under reduced pressure. Chromatography (SiO₂, 2% CMA/CHCl₃) gave 0.50 g of the desired product which was combined with the first batch of material for the next reaction.

Part B

2-[2-(Benzyloxy)ethyl]-1-(2-fluoro-2-methylpropyl)-1H-imidazo[4,5-c][1,5]naphthyridine (1.89 g, 5.0 mmol) was dissolved in 50 mL of CH₂Cl₂and treated with 3-chloroperoxybenzoic acid (1.50 g, 57-86% purity). After stirring for 2 hours, the reaction mixture was treated with 50 mL of 2% Na₂CO₃solution and the layers were separated. The aqueous portion was extracted with an additional 25 mL of CH₂Cl₂. The combined organic layers were washed successively with 2% Na₂CO₃, H₂O and brine, dried over Na₂SO₄, filtered, and concentrated under reduced pressure to give 1.97 g of 2-[2-(benzyloxy)ethyl]-1-(2-fluoro-2-methylpropyl)-1H-imidazo[4,5-c][1,5]naphthyridine 5-oxide as an off-white solid.

Part C

2-[2-(Benzyloxy)ethyl]-1-(2-fluoro-2-methylpropyl)-1H-imidazo[4,5-c][1,5]naphthyridine 5-oxide (1.97 g, 5.00 mmol) was dissolved in 50 mL of CH₂Cl₂and treated with 5 mL of concentrated aqueous NH₄OH solution. The mixture was stirred rapidly and then p-toluenesulfonyl chloride (1.00 g, 5.33 mmol) was carefully added. Rapid stirring was continued for 1 hour. The reaction mixture was then treated with 20 mL of H₂O. The layers were separated and the organic portion was washed successively with 5% Na₂CO₃solution, H₂O and brine. The organic portion was dried over Na₂SO₄, filtered, and concentrated under reduced pressure. Chromatography (SiO₂, 10% CMA/CHCl₃) gave 0.90 g of 2-[2-(benzyloxy)ethyl]-1-(2-fluoro-2-methylpropyl)-1H-imidazo[4,5-c][1,5]naphthyridin-4-amine as a yellow solid.

Part D

A solution of 2-[2-(benzyloxy)ethyl]-1-(2-fluoro-2-methylpropyl)-1H-imidazo[4,5-c][1,5]naphthyridin-4-amine (0.78 g, 1.98 mmol) dissolved in 20 mL of methanol was treated with 10% palladium on carbon (200 mg) and 0.68 mL of 3 M HCl in ethanol. The mixture was shaken under H₂at 50 PSI (3.4×10⁵Pa) overnight. Additional 10% palladium on carbon (200 mg) and 3 M HCl in ethanol (0.33 mL) were added and shaking was continued for 24 hours. The reaction mixture was filtered through a pad of CELITE filter agent. The pad was rinsed with methanol and the combined filtrates were concentrated under reduced pressure. The resulting material was treated with 20 mL of H₂O and 2 mL of concentrated NH₄OH solution and extracted into CHCl₃(3×25 mL). The combined organic layers were concentrated under reduced pressure. Chromatography (SiO₂, 15-30% CMA/CHCl₃) gave a white powder. Crystallization from ethanol/H₂O gave 276 mg of 2-[4-amino-1-(2-fluoro-2-methylpropyl)-1H-imidazo[4,5-c][1,5]naphthyridin-2-yl]ethanol as white needles, mp 224-225° C.

¹H NMR (300 MHz, DMSO-d₆, 354 K) δ 8.470 (dd, J=1.3, 4.0 Hz, 1H), 7.91 (d, J=8.4 Hz, 1H), 7.40 (dd, J=4.1, 8.3 Hz, 1H), 6.46 (s, 2H), 5.25 (d, J=22.7 Hz, 2H), 4.57 (s, 1H), 3.91 (d, J=5.4 Hz, 2H), 3.14 (t, J=6.4 Hz, 2H), 1.33 (d, J=21.7 Hz, 6H); ¹³C NMR (125 MHz, DMSO-d₆) δ 154.0, 152.3, 143.0, 140.4, 134.1, 133.1, 132.6, 129.0, 122.2, 96.7 (d, J=170 Hz), 60.2, 52.5 (d, J=20.9 Hz), 30.6 (d, J=6.6 Hz), 24.4; MS (ESI) m/z 304 (M+H)⁺; Anal. calcd for C₁₅H₁₈FN₅O: C, 59.39; H, 5.98; N, 23.09. Found: C, 59.57; H, 5.75; N, 23.07.

Examples 553-593
Part A

A solution of 1-(2-aminoethyl)-2-(2-methoxyethyl)-1H-imidazo[4,5-c][1,5]naphthyridin-4-amine (57 mg, 0.1 mmol, 1 eq, prepared according to the general method of Example 146 using methoxypropionyl chloride in lieu of methoxyacetyl chloride) and N,N-diisopropylethylamine (87 μL) in N,N-dimethylacetamide (1 mL) was added to a tube containing a reagent (1.1 eq) from the table below. The reaction mixture was vortexed overnight, the reaction was quenched with water (2 drops), and the solvent was removed by vacuum centrifugation. The reaction mixture was purified by solid-supported liquid-liquid extraction according to the following procedure. The sample was dissolved in chloroform (1 mL) then loaded onto diatomaceous earth that had been equilibrated with 2 M sodium carbonate solution (600 μL) for about 20 minutes. After 10 minutes chloroform (500 μL) was added to elute the product from the diatomaceous earth into a well of a collection plate. After an additional 10 minutes the process was repeated with additional chloroform (500 μL). The solvent was then removed by vacuum centrifugation.

Part B

The material from Part A was dissolved in dichloromethane (1 mL) and the solution was cooled to 0° C. Boron tribromide (400 μL of 1 M in dichloromethane) was added and the reaction mixture was vortexed overnight. Methanol (1 mL) and 6 N hydrochloric acid (500 μL) were added and the reaction mixture was vortexed for 15 minutes. The solvent was removed by vacuum. The compounds were purified as described for Examples 150-155. The table below shows the reagent used for each example, the structure of the resulting compound, and the observed accurate mass for the isolated trifluoroacetate salt.

Measured Mass

Example
Reagent
R
(M + H)

553
None

273.1479

554
Cyclopropanecarbonylchloride

341.1730

555
Isobutyryl chloride

343.1909

556
Cyclobutanecarbonylchloride

355.1909

557
Cyclopentanecarbonylchloride

369.2062

558
Benzoyl chloride

377.1747

559
Cyclohexanecarbonylchloride

383.2206

560
3-Cyanobenzoyl chloride

402.1702

561
4-Cyanobenzoyl chloride

402.1700

562
Cinnamoyl chloride

403.1890

563
Hydrocinnamoylchloride

405.2044

564
2-Methoxybenzoylchloride

393.1672

565
3-Methoxybenzoylchloride

393.1689

566
p-Anisoyl chloride

393.1678

567
2-Chlorobenzoylchloride

411.1306

568
3-Chlorobenzoylchloride

411.1369

569
4-Chlorobenzoylchloride

411.1368

570
Isonicotinoyl chloridehydrochloride

378.1698

571
Nicotinoyl chloridehydrochloride

378.1676

572
Methanesulfonylchloride

351.1256

573
Ethanesulfonyl chloride

365.1386

574
1-Propanesulfonylchloride

379.1534

575
Dimethylsulfamoylchloride

380.1512

576
Benzenesulfonylchloride

413.1436

577
1-Methylimidazole-4-sulfonyl chloride

417.1462

578
2,2,2-Trifluoroethanesulfonylchloride

419.1139

579
alpha-Toluenesulfonylchloride

427.1569

580
3-Cyanobenzenesulfonylchloride

438.1380

581
3-Methoxybenzenesulfonylchloride

429.1349

582
2-Chlorobenzenesulfonylchloride

447.0996

583
4-Chlorobenzenesulfonylchloride

447.1031

584
Isopropyl isocyanate

358.1994

585
Phenyl isocyanate

392.1794

586
Cyclohexyl isocyanate

398.2305

587
(R)-(+)-alpha-Methylbenzyl isocyanate

420.2178

588
(S)-(−)-alpha-Methylbenzyl isocyanate

420.2149

589
2-Chlorophenylisocyanate

426.1453

590
4-Chlorophenylisocyanate

426.1460

591
N,N-Dimethylcarbamoylchloride

344.1856

592
1-Piperidinecarbonylchloride

384.2137

593
4-Morpholinylcarbonylchloride

386.1976

Examples 594-632

The compounds in the table below were prepared and purified according to the methods described in Examples 553-593 using 1-(2-aminoethyl)-2-methoxymethyl-1H-imidazo[4,5-c][1,5]naphthyridin-4-amine in lieu of 1-(2-aminoethyl)-2-(2-methoxyethyl)-1H-imidazo[4,5-c][1,5]naphthyridin-4-amine. The table below shows the reagent used for each example, the structure of the resulting compound, and the observed accurate mass for the isolated trifluoroacetate salt.

Measured Mass

Example
Reagent
R
(M + H)

594
Cyclopropanecarbonylchloride

327.1581

595
Isobutyryl chloride

329.1709

596
Cyclopentanecarbonylchloride

355.1859

597
Benzoyl chloride

363.1563

598
Cyclohexanecarbonylchloride

369.2019

599
3-Cyanobenzoylchloride

388.1517

600
Hydrocinnamoylchloride

391.1868

601
3-Methoxybenzoylchloride

379.1512

602
p-Anisoyl chloride

379.1526

603
2-Chlorobenzoylchloride

397.1193

604
3-Chlorobenzoylchloride

397.1198

605
Isonicotinoyl chloridehydrochloride

364.1515

606
Nicotinoyl chloridehydrochloride

364.1535

607
Picolinoyl chloridehydrochloride

364.1512

608
trans-2-Phenyl-1-cyclopropanecarbonylchloride

403.1852

609
Methanesulfonylchloride

337.1070

610
Ethanesulfonylchloride

351.1212

611
1-Propanesulfonylchloride

365.1386

612
Isopropylsulfonylchloride

365.1433

613
Dimethylsulfamoylchloride

366.1355

614
Benzenesulfonylchloride

399.1214

615
1-Methylimidazole-4-sulfonyl chloride

403.1311

616
2,2,2-Trifluoroethanesulfonylchloride

405.0953

617
3-Cyanobenzenesulfonylchloride

424.1229

618
2-Chlorobenzenesulfonylchloride

433.0872

619
3-Chlorobenzenesulfonylchloride

433.0867

620
4-Chlorobenzenesulfonylchloride

433.0853

621
Methyl isocyanate

316.1528

622
Ethyl isocyanate

330.1660

623
Isopropyl isocyanate

344.1819

624
n-Propyl isocyanate

344.1809

625
Cyclopentyl isocyanate

370.1994

626
Cyclohexyl isocyanate

384.2152

627
3-Chlorophenylisocyanate

412.1300

628
4-Chlorophenylisocyanate

412.1273

629
N,N-Dimethylcarbamoylchloride

330.1686

630
1-Piperidinecarbonylchloride

370.1979

631
4-Morpholinylcarbonylchloride

372.1811

632
4-Methyl-1-piperazinecarbonylchloride

385.2098

Example 633
[4-Amino-1-(2-methylpropyl)-1H-imidazo[4,5-c][1,5]naphthyridin-2-yl]methanol

To a chilled solution (ice bath) of 2-(ethoxymethyl)-1-(2-methylpropyl)-1H-imidazo[4,5-c][1,5]naphthyridin-4-amine (2.0 g, 6.69 mmol, prepared according to the general methods of Example 148 using 2-methylpropan-1-amine in lieu of 1-amino-2-methylpropan-2-ol) in dichloromethane (50 mL) was added boron tribromide (20 mL, 1M solution in dichloromethane). The mixture turned light purple and was stirred at ambient temperature for 44 hours. The reaction was quenched with methanol (20 mL) and aqueous hydrochloric acid (6N, 10 mL). After stirring for 4 hours, the pH was adjusted to 10 by the addition of aqueous sodium hydroxide (50%). Dichloromethane (50 mL) was added with stirring and the layers were separated. The aqueous fraction was extracted with chloroform (2×250 mL). The combined organic fractions were concentrated to provide 1.4 g of [4-amino-1-(2-methylpropyl)-1H-imidazo[4,5-c][1,5]naphthyridin-2-yl]methanol as a white powder, mp 226-228° C.

¹H NMR (300 MHz, DMSO-d₆) δ 8.52-8.51 (dd, J=1.6, 4.3 Hz, 1H), 7.93-7.89 (dd, J=1.6, 8.4 Hz, 1H), 7.46-7.42 (dd, J=4.3, 8.4 Hz, 1H), 6.83 (s, 2H), 5.69-5.65 (t, J=5.8 Hz, 1H), 4.79-4.77 (d, J=5.8 Hz, 2H), 4.74-4.71 (d, J=7.6 Hz, 2H), 2.44-2.39 (m, 1H), 0.91-0.88 (d, J=6.7 Hz, 6H);

Anal. calcd for C₁₄H₁₇N₅O: C, 61.98; H, 6.31; N, 25.81. Found: C, 61.26; H, 6.07; N, 25.75.

Example 634
2-(4-Amino-1-isobutyl-1H-imidazo[4,5-c]quinolin-2-yl)ethyl acetate

Part A

To a stirred suspension of N⁴-isobutylquinoline-3,4-diamine (13.0 g, 60.46 mmol) in toluene was added pyridine hydrochloride (2.1 g, 8.14 mmol) followed by 3-chloropropionyl chloride (1.1 equivalents). The creamy suspension was stirred for 4 hours at ambient temperature and the solvent was then evaporated under reduced pressure. The tan solid obtained was dissolved in chloroform and the solution was transferred to a separatory funnel. The organic layer was washed with water (1×) and brine (2×). The organic layer was separated, dried (MgSO₄), filtered, and the solvent was evaporated under reduced pressure to afford a tan solid (21 g). A portion of the tan solid (7 g) was taken up in acetic acid (110 mL) and the reaction was stirred at ambient temperature for 4 hours. The reaction was cooled in an ice-bath and 6M NaOH (300 mL) was added in portions to afford a creamy suspension. The reaction mixture was transferred to a separatory funnel and the product was extracted with chloroform (150 mL×2). The organic layers were combined, dried (MgSO₄), and filtered; and the filtrate was evaporated under reduced pressure to afford 2-(1-isobutyl-1H-imidazo[4,5-c]quinolin-2-yl)ethyl acetate as a brown oil (10 g) which was taken forward to the next step.

Part B

To a stirred solution of 2-(1-isobutyl-1H-imidazo[4,5-c]quinolin-2-yl)ethyl acetate (7.41 g, 22.9 mmol) in chloroform was added 3-chloroperoxybenzoic acid (77%, 10.3 g, 49.9 mmol) and the reaction was stirred at ambient temperature for 4 hours. The reaction was then transferred to a separatory funnel and washed with brine (2×). The organic layers were combined, dried (MgSO₄), and filtered; and the filtrate was evaporated under reduced pressure to afford the N-oxide (15 g) as a brown solid. The brown solid was dissolved in chloroform, cooled in an ice-bath, and trichloroacetyl isocyanate (6.4 g mL, 34.3 mmol) was added in a dropwise manner. The reaction was stirred for 1 hour after which an additional 1.5 equivalents of trichloroacetyl isocyanate was added and the reaction was stirred at ambient temperature overnight. The reaction was concentrated under reduced pressure and the residue was suspended in ethanol. Potassium ethoxide was added to this suspension and the reaction was stirred for 1 hour. The reaction was concentrated under reduced pressure, taken up in dichloromethane (250 mL) and transferred to a separatory funnel. The organic layer was washed with water (250 mL), separated from the aqueous, dried (MgSO₄), and filtered; and the filtrate was evaporated under reduced pressure to afford a brown solid. The product was isolated by prep HPLC (ISCO Combiflash Separation System, Biotage Si 40+M column, eluted with a gradient of 0-10% methanol in dichloromethane with 1% ammonium hydroxide) to provide a solid (about 7 g). A portion of this solid was recrystallized from acetonitrile to afford 2-(4-amino-1-isobutyl-1H-imidazo[4,5-c]quinolin-2-yl)ethyl acetate as a white solid (0.109 g), mp 187-189° C.; MS (ESI) m/z 327 (M+H); Anal. Calcd for C₁₈H₂₂N₄O₂.0.40H₂O: C, 64.81; H, 6.89; N, 16.79. Found C, 64.54; H, 6.46; N, 16.90.

Example 635
[4-Amino-1-(2-hydroxy-2-methylpropyl)-1H-imidazo[4,5-c]quinolin-2-yl]methyl acetate

To a round-bottomed flask with stir bar was added 1-(4-amino-2-hydroxymethyl-1H-imidazo[4,5-c]quinolin-1-yl)-2-methylpropan-2-ol (1 g, 3.5 mmol) followed by dichloroethane (20 mL) and pyridine (3 mL). To the stirred suspension was added acetyl chloride (0.27 mL, 1.1 equivalents) and the reaction was stirred at ambient temperature for 30 min. The solvent was evaporated under reduced pressure to afford a solid. The product was isolated by two purifications by prep HPLC (ISCO Combiflash Separation System, Biotage Si 40+M column, eluted with a gradient of 0-7% methanol in dichloromethane with 1% ammonium hydroxide) to provide [4-amino-1-(2-hydroxy-2-methylpropyl)-1H-imidazo[4,5-c]quinolin-2-yl]methyl acetate as an off white solid (130 mg), mp 203-205° C.; MS (ESI) m/z 329 (M+H); Anal. Calcd for C₁₇H₂₀N₄O₃.0.25CH₄O: C, 61.59; H, 6.29; N, 16.66. Found C, 61.24; H, 6.22; N, 16.97.

Example 636
[4-Amino-1-(2-hydroxy-2-methylpropyl)-1H-imidazo[4,5-c]quinolin-2-yl]methyl L-valinate

[4-Amino-1-(2-hydroxy-2-methylpropyl)-1H-imidazo[4,5-c]quinolin-2-yl]methyl L-valinate was prepared according to the general method used to prepare (4-amino-1-{4-{(methylsulfonyl)amino]butyl}-1H-imidazo[4,5-c]quinolin-2-yl)methyl L-valinate using 1-(4-amino-2-hydroxymethyl-1H-imidazo[4,5-c]quinolin-1-yl)-2-methylpropan-2-ol in lieu of N-[4-(4-amino-2-hydroxymethyl-1H-imidazo[4,5-c]quinolin-1-yl)butyl]methanesulfonamide. The product was provided as off-white needles, mp 190-192° C.; MS (ESI) m/z 386 (M+H); Anal. Calcd for C₂₀H₂₇N₅O₃: C, 62.32; H, 7.06; N, 18.17. Found C, 62.08; H, 7.11; N, 17.96.

Exemplary Compounds Useful in Practicing Methods of the Invention

Certain exemplary compounds, including some of those described above in the Examples, have the following Formulas Ia, Ib, Ic, Id, Ie, If, Ig, Ih, Ii, Ij, Ik, Im, In, Io, or Ip and the following substituents n and R₁wherein each line of the table is matched to Formula Ia, Ib, Ic, Id, Ie, If, Ig, Ih, Ii, Ij, Ik, Im, In, Io, or Ip to represent a specific compound which is useful in practicing methods of the invention.

Ia

Ib

Ic

Id

Ie

If

Ig

Ih

Ii

Ij

Ik

Im

In

Io

Ip

n
R₁

1
2-[(cyclohexylcarbonyl)amino]-2-methylpropyl

1
2-[(cyclopropylcarbonyl)amino]ethyl

1
4-[(cyclopropylcarbonyl)amino]butyl

1
2,3-dihydroxypropyl

1
2,2-dimethyl-3-(methylsulfonyl)propyl

1
2-fluoro-2-methylpropyl

1
2-hydroxy-2-methylpropyl

1
2-methylpropyl

1
2-methyl-2-({[(1-methylethyl)amino]carbonyl}amino)propyl

1
2-{[(1-methylethyl)carbonyl]amino}ethyl

1
4-{[(1-methylethyl)carbonyl]amino}butyl

1
2-methyl-2-[(methylsulfonyl)amino]propyl

1
4-[(methylsulfonyl)amino]butyl

1
2-[(methylsulfonyl)amino]ethyl

1
4-[(4-morpholinecarbonyl)amino]butyl

1
2-[(4-morpholinecarbonyl)amino]ethyl

1
tetrahydro-2H-pyran-4-ylmethyl

1
(4-hydroxytetrahydro-2H-pyran-4-yl)methyl

1
(1-hydroxycyclobutyl)methyl

1
(1-hydroxycyclopentyl)methyl

1
(1-hydroxycyclohexyl)methyl

2
2-[(cyclohexylcarbonyl)amino]-2-methylpropyl

2
2-[(cyclopropylcarbonyl)amino]ethyl

2
4-[(cyclopropylcarbonyl)amino]butyl

2
2,3-dihydroxypropyl

2
2,2-dimethyl-3-(methylsulfonyl)propyl

2
2-fluoro-2-methylpropyl

2
2-hydroxy-2-methylpropyl

2
2-methylpropyl

2
2-methyl-2-({[(1-methylethyl)amino]carbonyl}amino)propyl

2
2-{[(1-methylethyl)carbonyl]amino}ethyl

2
4-{[(1-methylethyl)carbonyl]amino}butyl

2
2-methyl-2-[(methylsulfonyl)amino]propyl

2
4-[(methylsulfonyl)amino]butyl

2
2-[(methylsulfonyl)amino]ethyl

2
4-[(4-morpholinecarbonyl)amino]butyl

2
2-[(4-morpholinecarbonyl)amino]ethyl

2
tetrahydro-2H-pyran-4-ylmethyl

2
(4-hydroxytetrahydro-2H-pyran-4-yl)methyl

2
(1-hydroxycyclobutyl)methyl

2
(1-hydroxycyclopentyl)methyl

2
(1-hydroxycyclohexyl)methyl

Cytokine Induction in Human Cells

An in vitro human blood cell system is used to assess cytokine induction. Activity is based on the measurement of interferon (α) and tumor necrosis factor (α) (IFN-α and TNF-α, respectively) secreted into culture media as described by Testerman et. al. in “Cytokine Induction by the Immunomodulators Imiquimod and S-27609”, Journal of Leukocyte Biology, 58, 365-372 (September, 1995).

Blood Cell Preparation for Culture

Whole blood from healthy human donors is collected by venipuncture into vacutainer tubes or syringes containing EDTA. Peripheral blood mononuclear cells (PBMC) are separated from whole blood by density gradient centrifugation using HISTOPAQUE-1077 (Sigma, St. Louis, Mo.) or Ficoll-Paque Plus (Amersham Biosciences Piscataway, N.J.). Blood is diluted 1:1 with Dulbecco's Phosphate Buffered Saline (DPBS) or Hank's Balanced Salts Solution (HBSS). Alternately, whole blood is placed in Accuspin (Sigma) or LeucoSep (Oreiner Bio-One, Inc., Longwood, Fla.) centrifuge frit tubes containing density gradient medium. The PBMC layer is collected and washed twice with DPBS or HBSS and re-suspended at 4×10⁶cells/mL in RPMI complete. The PBMC suspension is added to 96 well flat bottom sterile tissue culture plates containing an equal volume of RPMI complete media containing test compound.

Compound Preparation

The compounds are solubilized in dimethyl sulfoxide (DMSO). The DMSO concentration should not exceed a final concentration of 1% for addition to the culture wells. The compounds are generally tested at concentrations ranging from 30-0.014 μM. Controls include cell samples with media only, cell samples with DMSO only (no compound), and cell samples with reference compound.

Incubation

The solution of test compound is added at 60 μM to the first well containing RPMI complete and serial 3 fold dilutions are made in the wells. The PBMC suspension is then added to the wells in an equal volume, bringing the test compound concentrations to the desired range (usually 30-0.014 μM). The final concentration of PBMC suspension is 2×10⁶cells/mL. The plates are covered with sterile plastic lids, mixed gently and then incubated for 18 to 24 hours at 37° C. in a 5% carbon dioxide atmosphere.

Separation

Following incubation the plates are centrifuged for 10 minutes at 1000 rpm (approximately 200×g) at 4° C. The cell-free culture supernatant is removed and transferred to sterile polypropylene tubes. Samples are maintained at −30 to −70° C. until analysis. The samples are analyzed for IFN-α by ELISA and for TNF-α by IGEN/BioVeris Assay.

Interferon (α) and Tumor Necrosis Factor (α) Analysis

IFN-α concentration is determined with a human multi-subtype calorimetric sandwich ELISA (Catalog Number 41105) from PBL Biomedical Laboratories, Piscataway, N.J. Results are expressed in pg/mL.

The TNF-α concentration is determined by ORIGEN M-Series Immunoassay and read on an IGEN M-8 analyzer from BioVeris Corporation, formerly known as IGEN International, Gaithersburg, Md. The immunoassay uses a human TNF-α capture and detection antibody pair (Catalog Numbers AHC3419 and AHC3712) from Biosource International, Camarillo, Calif. Results are expressed in pg/mL.

Assay Data and Analysis

In total, the data output of the assay consists of concentration values of TNF-α and IFN-α (y-axis) as a function of compound concentration (x-axis).

Analysis of the data has two steps. First, the greater of the mean DMSO (DMSO control wells) or the experimental background (usually 20 pg/mL for IFN-α and 40 pg/mL for TNF-α) is subtracted from each reading. If any negative values result from background subtraction, the reading is reported as “*”, and is noted as not reliably detectable. In subsequent calculations and statistics, “*”, is treated as a zero. Second, all background subtracted values are multiplied by a single adjustment ratio to decrease experiment to experiment variability. The adjustment ratio is the area of the reference compound in the new experiment divided by the expected area of the reference compound based on the past 61 experiments (unadjusted readings). This results in the scaling of the reading (y-axis) for the new data without changing the shape of the dose-response curve. The reference compound used is 2-[4-amino-2-ethoxymethyl-6,7,8,9-tetrahydro-α,α-dimethyl-1H-imidazo[4,5-c]quinolin-1-yl]ethanol hydrate (U.S. Pat. No. 5,352,784; Example 91) and the expected area is the sum of the median dose values from the past 61 experiments.

The minimum effective concentration is calculated based on the background-subtracted, reference-adjusted results for a given experiment and compound. The minimum effective concentration (μmolar) is the lowest of the tested compound concentrations that induces a response over a fixed cytokine concentration for the tested cytokine (usually 20 pg/mL for IFN-α and 40 pg/mL for TNF-α). The maximal response (pg/mL) is the maximal response attained in the dose response curve.

Compounds used in the methods of the invention and close analogs were tested for their ability to induce cytokine biosynthesis using the test method described above. The analogs used are shown in the table below.

Analog
Chemical Name
Reference

1
N-[2-(4-Amino-2-methyl-1H-imidazo[4,5-c]quinolin-1-
U.S. Pat. No. 6,677,349^#

yl)-1,1-dimethylethyl]methanesulfonamide

2
N-[2-(4-Amino-2-ethyl-1H-imidazo[4,5-c]quinolin-1-
U.S. Pat. No. 6,677,349^#

yl)-1,1-dimethylethyl]methanesulfonamide

3
N-[2-(4-Amino-2-propyl-1H-imidazo[4,5-c]quinolin-1-
U.S. Pat. No. 6,677,349^#

yl)-1,1-dimethylethyl]methanesulfonamide

4
N-[2-(4-Amino-2-ethoxymethyl-1H-imidazo[4,5-
U.S. Pat. No. 6,677,349

c]quinolin-1-yl)-1,1-dimethylethyl]methanesulfonamide
Example 268

5
N-{2-[4-Amino-2-(2-methoxyethyl)-1H-imidazo[4,5-
Example 6 Part D

c]quinolin-1-yl]-1,1-dimethylethyl}methanesulfonamide

^#This compound is not specifically exemplified but can be readily prepare using the synthetic methods disclosed in the cited reference

The compounds of Examples 6 and 7 and several closely related analogs were tested using the test method described above. The IFN-α dose response curves for Example 6, Analog 2, Analog 3 and Analog 5 are shown in FIG. 1. The TNF-α dose response curves for Example 6, Analog 2, Analog 3 and Analog 5 are shown in FIG. 2. The IFN-α dose response curves for Example 7, Analog 1, Analog 2 and Analog 4 are shown in FIG. 3. The TNF-α dose response curves for Example 7, Analog 1, Analog 2 and Analog 4 are shown in FIG. 4. The minimum effective concentration for the induction of IFN-α, minimum effective concentration for the induction of TNF-α, the maximal response for IFN-α, and the maximal response for TNF-α are shown in Table 7 below where # is the number of separate experiments in which the compound was tested. When a compound was tested in more than one experiment the values shown are the median values.

TABLE 7

Minimum

Effective
Maximal

Concentration
Response

(μM)
(pg/mL)

Compound
R₂
IFN
TNF
IFN
TNF
#

Example 7
—CH₂OH
3.330
30.00
2250
121
5

Example 6
—(CH₂)₂OH
1.11
>30
7521
—
3

Analog 1
—CH₃
0.370
3.330
1846
1518
7

Analog 2
—CH₂CH₃
0.120
1.110
831
3670
4

Analog 3
—(CH₂)₂CH₃
0.120
0.370
832
7245
9

Analog 4
—CH₂OCH₂CH₃
0.040
0.370
889
10125
22

Analog 5
—(CH₂)₂OCH₃
0.014
0.12
825
12518
6

Further compounds used in the methods of the invention and close analogs were tested for their ability to induce cytokine biosynthesis using the test method described above. The analogs used are shown in the table below.

Analog
Chemical Name
Reference

6
1-(4-amino-2-ethoxymethyl-1H-imidazo[4,5-
Example 148

c][1,5]naphthyridin-1-yl)-2-methylpropan-2-ol
Part E

7
1-[4-amino-2-(2-methoxyethyl)-1H-imidazo[4,5-
Example 149

c][1,5]naphthyridin-1-yl]-2-methylpropan-2-ol
Part J

The compounds of Examples 148 and 149 and several closely related analogs were tested using the test method described above. The IFN-α dose response curves for Example 148, Example 149, Analog 6, and Analog 7 are shown in FIG. 5. The TNF-α dose response curves for Example 148, Example 149, Analog 6, and Analog 7 are shown in FIG. 6. The minimum effective concentration for the induction of IFN-α, minimum effective concentration for the induction of TNF-α, the maximal response for IFN-α, and the maximal response for TNF-α are shown in Table 8 below where # is the number of separate experiments in which the compound was tested. When a compound was tested in more than one experiment the values shown are the median values.

TABLE 8

Minimum

Effective
Maximal

Concentration
Response

(μM)
(pg/mL)

Compound
R₂
IFN
TNF
IFN
TNF
#

Example 148
—CH₂OH
1.11
10.0
3038
684
2

Example 149
—(CH₂)₂OH
3.33
30.0
1849
342
1

Analog 6
—CH₂OCH₂CH₃
0.12
1.11
658
4921
1

Analog 7
—(CH₂)₂OCH₃
0.04
0.37
4143
7762
1

A further compound used in the methods of the invention and close analogs were tested for their ability to induce cytokine biosynthesis using the test method described above. The analogs used are shown in the table below.

Analog
Chemical Name
Reference

8
1-(4-amino-2-ethyl-7-pyridin-3-yl-1H-imidazo[4,5-
U.S. Patent Publication

c]quinolin-1-yl)-2-methylpropan-2-ol
2004/0147543

Example 142

9
1-(4-amino-2-propyl-7-pyridin-3-yl-1H-
U.S. Patent Publication

imidazo[4,5-c]quinolin-1-yl)-2-methylpropan-2-ol
2004/0147543

Example 418

10
1-(4-amino-2-ethoxymethyl-7-pyridin-3-yl-1H-
U.S. Patent Publication

imidazo[4,5-c]quinolin-1-yl)-2-methylpropan-2-ol
2004/0147543

Example 126

The compound of Example 163 and several closely related analogs were tested using the test method described above. The IFN-α dose response curves are shown in FIG. 7. The TNF-α dose response curves are shown in FIG. 8. The minimum effective concentration for the induction of IFN-α, minimum effective concentration for the induction of TNF-α, the maximal response for IFN-α, and the maximal response for TNF-α are shown in Table 9 below where # is the number of separate experiments in which the compound was tested. When a compound was tested in more than one experiment the values shown are the median values.

TABLE 9

Minimum

Effective
Maximal

Concentration
Response

(μM)
(pg/mL)

Compound
R₂
IFN
TNF
IFN
TNF
#

Example 163
—CH₂OH
1.11
>30
2251
*
1

Analog 8
—CH₂CH₃
0.12
0.37
1118
3234
4

Analog 9
—(CH₂)₂CH₃
0.04
0.37
597
3951
1

Analog 10
—CH₂OCH₂CH₃
0.04
0.12
840
0.12
5

* Below experimental background level of 40 pg/mL.

Compounds of the invention and close analogs were tested for their ability to induce cytokine biosynthesis using the test method described above. The minimum effective concentration for the induction of IFN-α, minimum effective concentration for the induction of TNF-α, the maximal response for IFN-α, and the maximal response for TNF-α are shown in Table 10 below where # is the number of separate experiments in which the compound was tested. When a compound was tested in more than one experiment the values shown are the median values.

TABLE 10

Minimum Effective
Maximal Response

Concentration (μM)
(pg/mL)

Compound
R₂
R₁
R₃
IFN
TNF
IFN
TNF
#

Example 163

0.37
10
2886
51
3

Analog 8

0.12
0.37
1652
3571
6

Analog 9

0.04
0.37
597
3951
1

Analog 10

0.04
0.12
840
7867
7

Analog 11

0.37
1.11
829
3445
4

Analog 12

0.014
0.014
1065
8386
8

Example 189

0.37
>30
4357
*
3

Analog 13

0.12
3.33
1771
8000
4

Analog 14

0.014
0.12
6308
18284
4

Analog 15

0.014
1.11
2084
10087
5

Analog 16

0.014
0.04
5868
16296
2

Analog 17

0.014
0.12
1079
16482
2

Example 191

1.11
>30
969
*
1

Analog 18

0.12
0.37
2979
1449
2

Analog 19

0.12
1.11
1686
619
8

Analog 20

0.12
0.37
1157
1054
2

Example 156

>30
>30
*
*
1

Analog 21

0.12
1.11
1880
201
2

Analog 22

0.37
1.11
1665
62
1

Example 157

0.37
3.33
1274
67
1

Analog 23

0.014
0.014
260
2296
1

Analog 24

0.014
0.12
440
2238
1

Example 158

0.37
3.33
1180
42
1

Analog 25

0.014
0.04
1199
3151
3

Analog 26

0.014
0.12
591
647
1

Example 159

0.12
10
1891
349
1

Analog 27

0.014
0.04
1332
9563
2

Analog 28

0.04
0.37
1263
3885
3

Example 195

0.37
30
5089
81
1

Analog 29

0.04
1.11
936
1059
2

Analog 30

0.37
0.37
531
5284
1

Example 196

0.12
>30
3516
*
1

Analog 31

0.12
1.11
965
991
2

Analog 32

0.12
0.37
862
1647
2

Example 197

0.04
10
4373
600
1

Analog 33

0.014
1.11
925
1618
2

Analog 34

0.014
0.37
649
9019
1

Example 198

0.12
3.33
2745
410
1

Analog 35

0.04
0.37
969
1366
2

Analog 36

0.12
0.37
521
2222
1

Example 199

0.37
10
5880
217
1

Analog 37

0.12
1.11
1194
728
2

Analog 38

0.12
0.37
1610
960
2

Example 160

30
>30
109
*
1

Analog 39

0.12
1.11
753
380
3

Example 161

>30
>30
*
*
1

Analog 40

0.37
3.33
1179
943
3

Example 164

30
>30
87
*
1

Analog 41

0.014
0.12
541
10184
1

Example 165

0.37
0.37
1681
7423
1

Analog 42

0.12
0.12
650
4456
1

Example 168

0.37
10
12641
352
1

Analog 43

0.04
0.04
740
3955
1

Example 201

>30
>30
*
*
1

Analog 44

0.04
1.11
1382
3128
1

Example 205

3.33
>30
1087
*
1

Analog 45

0.014
1.11
1062
2865
2

Example 206

1.11
>30
1266
*
1

Analog 46

0.014
0.37
815
1054
1

* Below experimental background level

All analogs are either specifically exemplified in or are readily prepared using the synthetic methods disclosed in U.S. Patent Application Publication 2004/0147543

Compounds of the invention and close analogs were tested for their ability to induce cytokine biosynthesis using the test method described above. The minimum effective concentration for the induction of IFN-α, minimum effective concentration for the induction of TNF-α, the maximal response for IFN-α, and the maximal response for TNF-α are shown in Table 11 below where # is the number of separate experiments in which the compound was tested. When a compound was tested in more than one experiment the values shown are the median values.

TABLE 11

Minimum Effective
Maximal Response

Concentration (μM)
(pg/mL)

Compound
R₁
R₂
IFN
TNF
IFN
TNF
#

Example 7
—CH₂C(CH₃)₂NHS(O)₂CH₃
—CH₂OH
3.33
30
1670
154
6

Example 6
—CH₂C(CH₃)₂NHS(O)₂CH₃
—(CH₂)₂OH
1.11
30
6527
*
4

Analog 1
—CH₂C(CH₃)₂NHS(O)₂CH₃
—CH₃
0.37
3.33
1846
1518
9

Analog 2
—CH₂C(CH₃)₂NHS(O)₂CH₃
—CH₂CH₃
0.12
1.11
1096
9675
6

Analog 3
—CH₂C(CH₃)₂NHS(O)₂CH₃
—CH₂CH₂CH₃
0.12
0.37
832
9780
11

Analog 4
—CH₂C(CH₃)₂NHS(O)₂CH₃
—CH₂OCH₂CH₃
0.04
0.37
1138
10665
33

Analog 5
—CH₂C(CH₃)₂NHS(O)₂CH₃
—(CH₂)₂OCH₃
0.014
0.12
1308
13908
8

Analog 47
—CH₂C(CH₃)₂NHS(O)₂CH₃
—CH₂OCH₃
0.37
3.33
1638
7151
1

Example 368
—(CH₂)₄NHS(O)₂CH₃
—CH₂OH
0.37
>30
7220
*
3

Example 3
—(CH₂)₄NHS(O)₂CH₃
—(CH₂)₂OH
0.37
>30
2340
*
4

Analog 48
—(CH₂)₄NHS(O)₂CH₃
—CH₃
0.12
10
7293
526
13

Analog 49
—(CH₂)₄NHS(O)₂CH₃
—CH₂CH₃
0.04
3.33
2712
679
79

Analog 50
—(CH₂)₄NHS(O)₂CH₃
—CH₂CH₂CH₃
0.12
1.11
2184
850
22

Analog 51
—(CH₂)₄NHS(O)₂CH₃
—CH₂OCH₂CH₃
0.04
1.11
2581
1439
10

Analog 52
—(CH₂)₄NHS(O)₂CH₃
—(CH₂)₂OCH₃
0.014
0.37
7594
1931
13

Example 115

—(CH₂)₂OH
1.11
>30
8361
*
1

Analog 53

—CH₃
0.12
10
1538
1400
1

Analog 54

—CH₂CH₃
0.37
3.33
4975
2570
1

Analog 55

—CH₂CH₂CH₃
0.12
1.11
11255
1298
3

Analog 56

—CH₂OCH₂CH₃
0.12
1.11
3433
1580
2

Analog 57

—(CH₂)₂OCH₃
0.014
0.04
8889
3494
8

Example 122
—(CH₂)₃NHS(O)₂CH₃
—(CH₂)₂OH
3.33
>30
9651
*
3

Aanalog 58
—(CH₂)₃NHS(O)₂CH₃
—CH₃
1.11
30
2778
*
11

Analog 59
—(CH₂)₃NHS(O)₂CH₃
—CH₂CH₃
1.11
30
1912
238
2

Analog 60
—(CH₂)₃NHS(O)₂CH₃
—CH₂CH₂CH₃
1.11
10
2148
109
3

Analog 61
—(CH₂)₃NHS(O)₂CH₃
—CH₂OCH₂CH₃
0.37
10
1338
463
9

Analog 62
—(CH₂)₃NHS(O)₂CH₃
—(CH₂)₂OCH₃
0.014
1.11
3995
954
9

Example 131
—CH₂C(CH₃)₂CH₂NHS(O)₂CH₃
—(CH₂)₂OH
0.37
>30
8361
*
1

Analog 63
—CH₂C(CH₃)₂CH₂NHS(O)₂CH₃
—CH₃
0.37
10
1019
805
2

Analog 64
—CH₂C(CH₃)₂CH₂NHS(O)₂CH₃
—CH₂CH₃
0.12
3.33
1431
1453
3

Analog 65
—CH₂C(CH₃)₂CH₂NHS(O)₂CH₃
—CH₂CH₂CH₃
0.12
10
1711
1929
2

Analog 66
—CH₂C(CH₃)₂CH₂NHS(O)₂CH₃
—CH₂OCH₂CH₃
0.12
0.37
561
3768
5

Analog 67
—CH₂C(CH₃)₂CH₂NHS(O)₂CH₃
—(CH₂)₂OCH₃
0.014
0.04
1805
5467
10

Example 36
—(CH₂)₂NHS(O)₂CH₃
—(CH₂)₂OH
10
>30
3316
*
1

Analog 68
—(CH₂)₂NHS(O)₂CH₃
—CH₃
0.12
10
1610
820
3

Analog 69
—(CH₂)₂NHS(O)₂CH₃
—CH₂CH₃
0.12
10
1610
820
3

Analog 70
—(CH₂)₂NHS(O)₂CH₃
—CH₂CH₂CH₃
30
10
2003
11432
2

Analog 71
—(CH₂)₂NHS(O)₂CH₃
—CH₂OCH₂CH₃
0.12
3.33
1465
4918
9

Analog 72
—(CH₂)₂NHS(O)₂CH₃
—(CH₂)₂OCH₃
0.014
0.04
5858
8547
6

Example 125
—(CH₂)₅S(O)₂CH₃
—(CH₂)₂OH
0.37
>30
8361
*
1

Analog 73
—(CH₂)₅S(O)₂CH₃
—CH₃
0.37
3.33
1294
771
21

Analog 74
—(CH₂)₅S(O)₂CH₃
—CH₂CH₃
0.12
1.11
1062
1545
7

Analog 75
—(CH₂)₅S(O)₂CH₃
—CH₂CH₂CH₃
0.12
1.11
828
848
3

Analog 76
—(CH₂)₅S(O)₂CH₃
—(CH₂)₂OCH₃
0.014
1.11
2695
6169
2

Example 133
—(CH₂)₂O(CH₂)₂N(CH₃)S(O)₂CH₃
—(CH₂)₂OH
0.37
>30
8361
*
1

Analog 77
—(CH₂)₂O(CH₂)₂N(CH₃)S(O)₂CH₃
—CH₃
0.12
1.11
1001
3571
1

Analog 78
—(CH₂)₂O(CH₂)₂N(CH₃)S(O)₂CH₃
—CH₂CH₃
0.12
1.11
1803
2525
1

Analog 79
—(CH₂)₂O(CH₂)₂N(CH₃)S(O)₂CH₃
—CH₂CH₂CH₃
0.37
3.33
1055
1312
2

Analog 80
—(CH₂)₂O(CH₂)₂N(CH₃)S(O)₂CH₃
—(CH₂)₂OCH₃
0.014
0.37
1630
2191
4

Example 99
—(CH₂)₃NHC(O)NHCH(CH₃)₂
—(CH₂)₂OH
0.37
>30
21829
*
1

Analog 81
—(CH₂)₃NHC(O)NHCH(CH₃)₂
—CH₃
3.33
10
1134
490
1

Analog 82
—(CH₂)₃NHC(O)NHCH(CH₃)₂
—CH₂CH₂CH₃
0.12
1.11
6571
3740
2

Analog 83
—(CH₂)₃NHC(O)NHCH(CH₃)₂
—(CH₂)₂OCH₃
0.12
1.11
1289
1259
1

Example 120
—(CH₂)₃NH₂
—(CH₂)₂OH
3.33
>30
5636
*
1

Analog 84
—(CH₂)₃NH₂
—CH₃
3.33
>30
421
*
1

Analog 85
—(CH₂)₃NH₂
—CH₂OCH₂CH₃
0.12
30
1325
411
1

Analog 86
—(CH₂)₃NH₂
—(CH₂)₂OCH₃
0.04
1.11
3433
1674
1

Example 128

—(CH₂)₂OH
30
>30
75
*
3

Analog 87

—CH₃
0.37
30
4843
463
2

Analog 88

—CH₂OCH₂CH₃
0.12
1.11
6670
1379
2

Analog 89

—(CH₂)₂OCH₃
0.014
0.014
5915
6169
2

Example 130

—(CH₂)₂OH
0.014
3.33
8361
2001
1

Analog 90

—CH₂CH₃
0.014
0.12
922
2098
2

Analog 91

—CH₂OCH₂CH₃
0.014
0.04
1133
3618
2

Analog 92

—(CH₂)₂OCH₃
0.014
0.04
570
6449
2

Example 5

—CH₂OH
0.37
10
17274
1130
1

Analog 93

—CH₂OCH₂CH₃
0.37
0.37
1052
12173
13

Analog 94

—CH₂OCH₃
1.11
3.33
2518
9721
1

Example 124

—(CH₂)₂OH
0.12
3.33
3980
1446
1

Analog 95

—CH₂OCH₂OCH₃
0.04
0.37
832
1820
5

Analog 96

—(CH₂)₂OCH₃
0.014
0.014
2133
1812
1

Example 126
—(CH₂)₃NHC(O)NH(CH₂)₃CH₃
—(CH₂)₂OH
1.11
>30
8361
*
1

Analog 97
—(CH₂)₃NHC(O)NH(CH₂)₃CH₃
—CH₂OCH₂CH₃
0.37
3.33
827
963
5

Analog 98
—(CH₂)₃NHC(O)NH(CH₂)₃CH₃
—(CH₂)₂OCH₃
0.014
0.04
5915
6169
2

Example 129
—CH₂C(CH₃)₂CH₂NH₂
—(CH₂)₂OH
0.37
30
2702
85
1

Analog 99
—CH₂C(CH₃)₂CH₂NH₂
—CH₂CH₃
0.04
0.37
405
13846
1

Analog 100
—CH₂C(CH₃)₂CH₂NH₂
—(CH₂)₂OCH₃
0.014
0.04
571
17626
1

Example 132

—(CH₂)₂OH
0.37
>30
8361
*
1

Analog 101

—CH₃
1.11
3.33
571
156
3

Analog 102

—(CH₂)₂OCH₃
0.014
1.11
1504
3080
2

Example 137
—(CH₂)₂NHC(O)NHCH₂CH₃
—(CH₂)₂OH
30
30
801
73
1

Analog 103
—(CH₂)₂NHC(O)NHCH₂CH₃
—CH₂CH₃
3.33
10
1031
3250
2

Analog 104
—(CH₂)₂NHC(O)NHCH₂CH₃
—(CH₂)₂OCH₃
0.014
0.12
2587
7719
4

Example 138
—(CH₂)₂NHC(O)CH₂CH(CH₃)₂
—(CH₂)₂OH
3.33
>30
36
*
1

Analog 105
—(CH₂)₂NHC(O)CH₂CH(CH₃)₂
—CH₂CH₃
3.33
30
851
587
2

Analog 106
—(CH₂)₂NHC(O)CH₂CH(CH₃)₂
—(CH₂)₂OCH₃
0.12
3.33
1204
5694
5

Example 142
—CH₂C(CH₃)₂NHC(O)NHCH(CH₃)₂
—CH₂OH
1.11
>30
1554
*
1

Analog 107
—CH₂C(CH₃)₂NHC(O)NHCH(CH₃)₂
—CH₂CH₂CH₃
1.11
3.33
1428
6363
3

Analog 108
—CH₂C(CH₃)₂NHC(O)NHCH(CH₃)₂
—CH₂OCH₂CH₃
0.37
1.11
966
10587
4

Example 1

—(CH₂)₂OH
0.37
10
1072
143
1

Analog 109

—(CH₂)₂OCH₃
0.04
0.37
638
6169
2

Example 2

—(CH₂)₂OH
3.33
3.33
507
45
1

Analog 110

—(CH₂)₂OCH₃
0.12
1.11
647
6169
2

Example 4
—CH₂C(CH₃)₂NH₂
—CH₂OH
0.37
3.33
1893
41
2

Analog 111
—CH₂C(CH₃)₂NH₂
—CH₂OCH₂CH₃
0.12
0.37
656
11475
7

Example 111

—(CH₂)₂OH
0.12
1.11
7753
983
1

Analog 112

—(CH₂)₂OCH₃
0.014
0.04
2127
1462
7

Example 112

—(CH₂)₂OH
1.11
30
8361
76
1

Analog 113

—(CH₂)₂OCH₃
0.014
0.04
6032
3786
4

Example 114
—(CH₂)₄NH₂
—(CH₂)₂OH
30
>30
23
*
1

Analog 114
—(CH₂)₄NH₂
—(CH₂)₂OCH₃
0.04
0.37
127231
724
1

Example 116

—(CH₂)₂OH
0.37
30
8361
1112
1

Analog 115

—(CH₂)₂OCH₃
0.014
0.04
7545
9340
2

Example 117

—(CH₂)₂OH
0.37
3.33
5520
1938
1

Analog 116

—(CH₂)₂OCH₃
0.014
0.04
1129
7261
3

Example 118

—(CH₂)₂OH
0.37
>30
5177
*
1

Analog 117

—(CH₂)₂OCH₃
0.014
0.12
1257
1372
1

Example 119
—(CH₂)₈NHS(O)₂CH₃
—(CH₂)₂OH
0.04
3.33
8361
693
1

Analog 118
—(CH₂)₈NHS(O)₂CH₃
—(CH₂)₂OCH₃
0.014
0.014
1914
1853
2

Example 121

—(CH₂)₂OH
0.37
3.33
2441
180
1

Analog 119

—(CH₂)₂OCH₃
0.014
0.014
1584
1995
1

Example 134

—(CH₂)₂OH
3.33
30
8361
315
1

Analog 120

—(CH₂)₂OCH₃
0.04
0.37
1394
3317
1

Example 135

—(CH₂)₂OH
3.33
30
2464
146
1

Analog 121

—(CH₂)₂OCH₃
0.37
1.11
1234
4849
2

Example 140
—(CH₂)₂O(CH₂)₂NHC(O)(CH₂)₁₄CH₃
—(CH₂)₂OH
1.11
>30
673
*
1

Analog 121
—(CH₂)₂O(CH₂)₂NHC(O)(CH₂)₁₄CH₃
—(CH₂)₂OCH₃
0.014
0.014
2556
11033
9

Example 141
—(CH₂)₃NHC(O)CH(CH₃)₂
—(CH₂)₂OH
0.04
30
14046
243
1

Analog 123
—(CH₂)₃NHC(O)CH(CH₃)₂
—CH₃
1.11
10
3011
405
2

Example 364
—CH₂C(CH₃)₂CH₂S(O)₂CH₃
—CH₂OH
1.11
30
5343
164
1

Analog 124
—CH₂C(CH₃)₂CH₂S(O)₂CH₃
—CH₂OCH₂CH₃
0.12
0.37
1924
9513
4

Example 365
—(CH₂)₂NHC(O)NHCH(CH₃)₂
—CH₂OH
0.37
3.33
1488
74
1

Analog 125
—(CH₂)₂NHC(O)NHCH(CH₃)₂
—CH₂OCH₂CH₃
0.37
10
2045
7512
7

*TNF below experimental background of 40 pg/mL

Analogs 1-5, 47-52, 58-74, 109, 113, and 118 are either specifically exemplified in or are readily prepared using the synthetic methods disclosed in U.S. Pat. Nos. 6,331,539 and 6,677,349.

Analogs 53-57, 81-83, 87-91, 97, 98, 103, 104, 107, and 108 are either specifically exemplified in or are readily prepared using the synthetic methods disclosed in U.S. Pat. Nos. 6,541,485 and 6,573,273.

Analogs 73-76 and 124 are either specifically exemplified in or are readily prepared using the synthetic methods disclosed in U.S. Pat. No. 6,664,264.

Analogs 77-80 are either specifically exemplified in or are readily prepared using the synthetic methods disclosed in U.S. Pat. No. 6,683,088.

Analogs 84-86, 99, 100, 111, and 114 are either specifically exemplified in or are readily prepared using the synthetic methods disclosed in U.S. Pat. Nos. 6,069,149 and 6,677,349.

Analogs 93-96, 101, 102, 105, 106, 110, 112, 115, 116, 119, and 123 are either specifically exemplified in or are readily prepared using the synthetic methods disclosed in U.S. Pat. Nos. 6,451,810 and 6,756,382.

Analogs 120-122 are either specifically exemplified in or are readily prepared using the synthetic methods disclosed in U.S. Pat. No. 6,664,265.

Compounds of the invention and in some instances, close analogs (Table 13 below), were tested for their ability to induce cytokine biosynthesis using the test method described above. The minimum effective concentration for the induction of IFN-α, minimum effective concentration for the induction of TNF-α, the maximal response for IFN-α, and the maximal response for TNF-α are shown in Table 12 below where # is the number of separate experiments in which the compound was tested. When a compound was tested in more than one experiment the values shown are the median values.

TABLE 12

Minimum Effective
Maximal Response

Concentration (μM)
(pg/mL)

Compound
R₁
R₂
IFN
TNF
IFN
TNF
#

Example 148
—CH₂C(CH₃)₂OH
—CH₂OH
1.11
10
2290
1316
3

Example 149
—CH₂C(CH₃)₂OH
—(CH₂)₂OH
3.33
30
2063
331
2

Analog 6
—CH₂C(CH₃)₂OH
—CH₂OCH₂CH₃
0.12
1.11
1674
7275
2

Analog 7
—CH₂C(CH₃)₂OH
—(CH₂)₂OCH₃
0.04
0.37
3142
7503
2

Analog 126
—CH₂C(CH₃)₂OH
—CH₃
0.37
3.33
1952
6519
1

Analog 127
—CH₂C(CH₃)₂OH
—CH₂CH₃
0.37
3.33
2150
3863
1

Analog 128
—CH₂C(CH₃)₂OH
—CH₂CH₂CH₃
0.12
1.11
2484
5526
1

Example 143

—CH₂OH
1.11
10
1467
798
1

Analog 129

—CH₂OCH₂CH₃
0.014
0.014
1647
8691
1

Example 144
—CH₂C(CH₃)₂NHS(O)₂CH₃
—CH₂OH
10
30
1914
170
1

Analog 130
—CH₂C(CH₃)₂NHS(O)₂CH₃
—CH₂OCH₂CH₃
0.04
0.37
2465
9234
1

Example 551
—CH₂CF(CH₃)₂
—CH₂OH
1.11
3.33
1833
1922
1

Example 552
—CH₂CF(CH₃)₂
—(CH₂)₂OH
1.11
10
1646
84
1

Analog 131
—CH₂CF(CH₃)₂
—CH₃
0.37
10
2120
1679
2

Example 633
—CH₂CH(CH₃)₂
—CH₂OH
1.11
30
1592
363
1

Analog 132
—CH₂CH(CH₃)₂
—CH₂OCH₂CH₃
0.12
1.11
1524
3160
2

Analog 133
—CH₂CH(CH₃)₂
—CH₃
0.37
1.11
1117
699
12

Example 145
—(CH₂)₄NHS(O)₂CH₃
—(CH₂)₂OH
1.11
30
3008
7
2

Example 146
—(CH₂)₄NHS(O)₂CH₃
—CH₂OH
10
>30
1520
*
1

Example 147
—(CH₂)₄NHS(O)₂CH₃
—(CH₂)₂OH
30
>30
49
*
1

* Below the experimental background of 40 pg/mL

TABLE 13

Analog
Chemical Name
Reference

6
1-(4-amino-2-ethoxymethyl-1H-imidazo[4,5-
Example 148 Part E

c][1,5]naphthyridin-1-yl)-2-methylpropan-2-ol

7
1-[4-amino-2-(2-methoxyethyl)-1H-imidazo[4,5-
Example 149 Part J

c][1,5]naphthyridin-1-yl]-2-methylpropan-2-ol

126
1-(4-amino-2-methyl-1H-imidazo[4,5-
U.S. Pat. No.

c][1,5]naphthyridin-1-yl)-2-methylpropan-2-ol
6,194,425**

127
1-(4-amino-2-ethyl-1H-imidazo[4,5-
U.S. Pat. No.

c][1,5]naphthyridin-1-yl)-2-methylpropan-2-ol
6,194,425**

128
1-(4-amino-2-propyl-1H-imidazo[4,5-
U.S. Pat. No.

c][1,5]naphthyridin-1-yl)-2-methylpropan-2-ol
6,194,425**

129
N-[2-(4-amino-2-ethoxymethyl-1H-imidazo[4,5-
Example 143 Part H

c][1,5]naphthyridin-1-yl)-1,1-

dimethylethyl]cyclohexanecarboxamide

130
N-[2-(4-amino-2-ethoxoxymethyl-1H-imidazo[4,5-
Example 144 Part A

c][1,5]naphthyridin-1-yl)-1,1-

dimethylethyl]methanesulfonamide

131
1-(2-fluoro-2-methylpropyl)-2-methyl-1H-imidazo[4,5-
U.S. Pat. No.

c][1,5]naphthyridin-4-amine
6,194,425**

132
2-ethoxymethyl-1-(2-methylpropyl)-1H-imidazo[4,5-
U.S. Pat. No.

c][1,5]naphthyridin-4-amine
6,194,425**

133
2-methyl-1-(2-methylpropyl)-1H-imidazo[4,5-
U.S. Pat. No.

c][1,5]naphthyridin-4-amine
6,194,425

Example 36

**Although not a working example, the compound is readily prepared using the disclosed synthetic methods.

The complete disclosures of the patents, patent documents, and publications cited herein are incorporated by reference in their entirety as if each were individually incorporated. Various modifications and alterations to this invention will become apparent to those skilled in the art without departing from the scope and spirit of this invention. It should be understood that this invention is not intended to be unduly limited by the illustrative embodiments and examples set forth herein and that such examples and embodiments are presented by way of example only with the scope of the invention intended to be limited only by the claims set forth herein as follows.

Number	Date	Country
60655452	Feb 2005	US
60655508	Feb 2005	US
60655380	Feb 2005	US
60655495	Feb 2005	US

Method of Preferentially Inducing the Biosynthesis of Interferon

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Publication Number

Date Filed

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Inventors

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CPC

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International Classifications

Abstract

Description

Claims

CROSS REFERENCE TO RELATED APPLICATIONS

PCT Information

Provisional Applications (4)