Claims
- 1. A method of preventing particle aggregation of lipid:nucleic acid complex particles, said method comprising the step of incorporating an non-cationic lipid into lipid:nucleic acid complex particles comprising a cationic lipid and a nucleic acid polymer, wherein said additional lipid is a polyethylene glycol-based polymer.
- 2. The method of claim 1, wherein the polyethylene glycol-based polymer is selected from the group consisting of polyethylene glycol lipids, polyethylene glycol ceramides and ganglioside-modified lipids.
- 3. The method of claim 1, wherein the amount of additional lipid is from 1 to 15% of the particles.
- 4. The method of claim 1, wherein said nucleic acid is selected from the group consisting of DNA and RNA.
- 5. The method of claim 1, wherein said nucleic acid is DNA.
- 6. The method of claim 1, wherein said lipid:nucleic acid complex is lyophilized.
- 7. The method of claim 1, wherein the nucleic acid is linked to an expression vector to facilitate gene expression after entry into a cell.
- 8. A method of preparing a lipid:nucleic acid complex, comprising the steps of:
(a) combining a nucleic acid with a cationic lipid to produce a lipid:nucleic acid complex; and (b) mixing the lipid:nucleic acid complex with a non-cationic lipid which is a polyethylene glycol-based polymer, wherein the polyethylene glycol-based lipid reduces the tendency of the lipid:nucleic acid complex to aggregate.
- 9. The method of claim 8, wherein the polyethylene glycol-based polymer is selected from the group consisting of polyethylene glycol lipids, polyethylene glycol ceramides and ganglioside-modified lipids.
- 10. The method of claim 8, wherein the amount of non-cationic lipid is from 1 to 15%.
- 11. The method of claim 8, wherein said nucleic acid is selected from the group consisting of DNA and RNA.
- 12. The method of claim 8, wherein said nucleic acid is DNA.
- 13. The method of claim 8, wherein said lipid:nucleic acid complex is lyophilized.
- 14. The method of claim 8, wherein the nucleic acid is linked to an expression vector to facilitate gene expression after entry into a cell.
Parent Case Info
[0001] This application is a continuation of U.S. patent application Ser. No. 09/431,594, filed Jan. 11, 1999, and Ser. No. 09/566,700, filed Aug. 5, 2000, which are continuations of U.S. patent application Ser. No. 08/660,025, filed Jun. 6, 1996, now U.S. Pat. No. 5,976,567, which is a continuation-in-part of U.S. application Ser. No. 08/485,458, filed Jun. 7, 1995, and of U.S. application Ser. No. 08/484,282, filed on Jun. 7, 1995, now U.S. Pat. No. 5,705,385, all of which are incorporated herein by reference. .
Continuations (3)
|
Number |
Date |
Country |
Parent |
09431594 |
Nov 1999 |
US |
Child |
09875805 |
Jun 2001 |
US |
Parent |
09566700 |
May 2000 |
US |
Child |
09875805 |
Jun 2001 |
US |
Parent |
08660025 |
Jun 1996 |
US |
Child |
09566700 |
May 2000 |
US |
Continuation in Parts (2)
|
Number |
Date |
Country |
Parent |
08485458 |
Jun 1995 |
US |
Child |
08660025 |
Jun 1996 |
US |
Parent |
08484282 |
Jun 1995 |
US |
Child |
08660025 |
Jun 1996 |
US |