Method of producing microbial transglutaminase

Abstract

The present invention relates to a method of secretory production of transglutaminase by a microorganism.

Description

FIELD OF THE INVENTION

[0001] The present invention relates to a method for secretory production of actynomycetes-derived transglutaminase by genetic recombinant techniques using the host-vector system of Streptomyces lividans (also referred to as S. lividans hereinafter. Transglutaminase). Transglutaminase produced and secreted according to the present invention is widely used in the food processing or medicines.

[0002] Transglutaminase which is produced and secreted according to the present invention is an enzyme which catalyzes the acyl transfer reaction of γ-calboxylamide groups located in the peptide chain of proteins. When the enzyme is reacted with a protein, ε-(γ-Glu)-Lys cross linking reaction, the substitution reaction from Gln to Glu by deamination of Gln can be occurred. Transglutaminase is used for producing gelatinized foods such as jelly, or yogurt, cheese, gelatinized cosmetics or improving the quality of meat (Publication of unexamined Japanese patent application (referred to as JP-Kokai hereinafter) No. 1-50382). It is also used for producing materials for thermostable microcapsules or carriers for immobilized enzymes. Thus transglutaminase is an enzyme highly useful in the industry.

[0003] It is previously know that there are animal-derived transglutaminase and bacteria-derived transglutaminase (microbial transglutaminase, which may be referred to as MTG herein after). The former is a calcium dependent enzyme and distributes in the animal organs, skin or blood and the like. The examples are, for example, human keratinocyte transglutaminase (M. A. Phillips et al. Proc. Natl. Acad. Sci. USA, 87, 9333 (1990)), human blood coagulation factor XIII (A. Ichinose et al., Biochemistry, 25, 6900 (1990)). Regarding to the latter, calcium independent one is discovered from Streptoverticillium bacteria. The examples are, for example, Streptoverticillium griseocarneum IFO 12776, Streptoverticillium cinnamoneum sub sp. cinnamoneum (which may be referred to as S. cinnamoneum herein after) IFO 12852 and Streptoverticillium mobaraense (which may be referred to as S. mobaraense hereinafter) IFO 13819 (JP-Kokai No. 64-27471). Peptide mapping and genetic structural analysis revealed that transglutaminases produced by these bacteria have no homology with the enzymes from animals (EP 0 481 504 A1).

[0004] Microbial transglutaminases (MTGs) had problems in the amount or yield because they are produced through purification procedures from the culture of bacteria such as above described bacteria. The production of transglutaminase using genetic engineering techniques is also attempted. Transglutaminase proteins and genes thereof are described in, for example, JP-Kokai No. 64-27471, Biosci. Biotech. Biochem., 58, 82-87 (1994), Biosci. Biotech. Biochem., 58, 88-92 (1994), JP-Kokai No. 5-199883, Biochimie, 80, 313-319 (1998), Eur. J. Biochem., 257, 570-576 (1998), WO 9606931 and WO 9622366, which describe the reports for the production by host-vector system such as S. lividans, Aspergillus oryzae or Escherichia coli (which may be referred to as E. coli hereinafter). A producing method using secretory expression in microorganisms such as E. coli or yeast (JP-Kokai No. 5-199883) and a method for producing functional MTG by expressing MTG as an inactive fused protein inclusion body, resolubilizing the protein inclusion body by protein denaturing agent and then reconstituting the protein (JP-Kokai No.6-30771) are reported. However, in the secretory expression by conventional techniques using microorganisms, the problem of very poor expression level has been indicated. For secretory production of transglutaminase in Streptomyces, there is an report which describes an example including the specific description about secretory accumulation (Biosci. Biotech. Biochem., 58, 82-87 (1994); JP-Kokai No.5-199883), where transglutaminase gene from Streptoverticillium mobaraense was introduced into Streptoverticillium lividans as a host using genetic recombination techniques, however, the amount of the secretion was only about 0.1 mg/l.

SUMMARY OF THE INVENTION

[0005] It is an object of the present invention to provide a method of producing a large amount of transglutaminase by secretory production of transglutaminase in Streptomyces bacteria.

[0006] The method of the present-invention is the method of producing a large amount or transglutaminase characterized in that a host-vector system for Streptomyces is used, where an expression plasmid which can highly express a gene in Streptomyces bacteria is constructed using transglutaminase gene from Streptomyces, that is, the signal peptide region and the pro-structural region and mature structural region and the native (natural) promoter region controlling the expression of transglutaminase, and the expression plasmid is introduced into a Streptomyces bacterium, the bacterium is cultured, and the bacterium is directed to secrete transglutaminase having additional pro-structural part (pro-transglutaminase) during the initial phase to the middle phase of culturing and then mature (active) transglutaminase is obtained in the late phase of culturing by cleaving and removing the pro-structure, for example, with proteases derived from Streptomyces.

BRIEF DESCRIPTION OF THE DRAWINGS

[0007] FIG. 1. shows the construction procedure of the expression plasmid pUJ-MTG.

DETAILED DESCRIPTION OF THE INVENTION

[0008] According to the present invention, a large amount of mature (active form) transglutaminase are obtained by using a Streptomyces bacterium as a host-vector system and generating an expression construct containing the gene for transglutaminase having the pro-structural part (pro-transglutaminase) linked to the down stream of the native promoter for transglutaminase gene, and by introducing the construct into a Streptomyces bacterium, expressing it and cleaving the extracellulary secreted pro-transglutaminase, for example, with proteases produced by the same Streptomyces bacterium.

[0009] Secretory proteins are generally known to be translated as pre-peptides or prepro-peptides and then undergo post-translational modifications to generate mature proteins. Namely, it is generally known that secretory proteins are translated as pre-peptides or prepro-peptides, and then, the signal peptides (pre-part) thereof are cleaved to convert them into mature peptides or pro-peptides and the pro-parts of the pro-peptides are cleaved to generate the mature peptides. Transglutaminase is one of such proteins. As used herein, transglutaminase having both the signal peptide and the pro-part, namely the primary translational product, may be referred to as “prepro-transglutaminase” and transglutaminase having the pro-part but not the signal peptide may be referred to as “pro-transglutaminase”. The pro-part of pro-transglutaminase may be referred to as “pro-structural part” or simply “pro-structure”. As used herein, the “pro-structural part/pro-structure” of transglutaminase and the “pro-part” of the protein are used interchangeably. Thus, “pro-transglutaminase” may also be referred to as “transglutaminase with additional pro-structural part”.

[0010] As used herein, a protein of which pro-part is “cleaved and removed” refer to a protein wherein one or more amino acid constituting the pro-part is removed by cleaving peptide bond, and it includes a protein having the identical N-terminal region to the native mature protein and also a protein having one or more additional amino acid derived from the pro-part at its N-terminal compared to the native protein or a protein having shorter amino acid sequence than that of the native mature protein. As used herein, “mature transglutaminase” and “active form transglutaminase” are use in the same meaning.

[0011] Generally, the genetic constructs used in the present invention are those having suitable sequences containing promoters, nucleotide fragments encoding prepro-transglutaminase and regulatory sequences required to express the intended proteins in Streptomyces bacteria, at the appropriate location. The Vector which can be used for the constructs are not limited and any vector can be used which can function in Streptomyces bacteria, and the vectors may be vectors which autonomously replicate extrachromosomally such as plasmids or may be vectors which can integrated into the bacterial chromosome. Plasmids from Streptomyces are preferable, and the examples include, for example, pIJ702 (J. Gen. Microbiol., 129, 2703-2714, (1983)) and the plasmids obtained by improving it.

[0012] The promoters which can be used in the present invention to express transglutaminase genes in Streptomyces bacteria are the native promoters of transglutaminase genes from Actinomycetes. However, in some cases the promoter region cannot be identified, because the consensus sequence is not established in Actinomycetes in contrast with E.coli. In such cases, the genetic fragment containing 5′-upstream region large enough to covering the structural gene for transglutaminase and the promoter region required to regulate its expression may be used.

[0013] Transglutaminase genes which can be used in the present invention are not particularly limited, and, for example, genes for secretory transglutaminase derived from S. cinnamoneum IFO 12852, S. grosepcarneum IFO 12776, S. mobaraence IFO 13819 and Streptoverticillium lydicus (WO96/06931) are preferable. Transglutaminase genes from S. cinnamoneum or S. mobaraense are particularly preferable, which are used with the native promoter of the respective transglutaminase genes.

[0014] The entire nucleotide sequence of transglutaminase gene from S. cinnamoneum IF012852 containing the 5′-upstream region, which was identified by the inventors of the present invention, is shown in SEQ ID NO:1, and the amino acid sequence encoded by the nucleotide sequence is shown in SEQ ID NO:2. It was assumed that from amino acid at position 1 to position 32 in the amino acid sequence was the sequence for pre-part, from amino acid at position 33 to position 86 was the sequence for pro-part and from amino acid at position 87 to position 416 was the sequence for mature transglutaminase. The entire nucleotide sequence of transglutaminase gene containing the 5′-upstream region from S. mobaraense is also shown in SEQ ID NO:3, and the amino acid sequence encoded by the nucleotide sequence is shown in SEQ ID NO:4. From amino acid at position 1 to position 31 in the amino acid sequence is the sequence for pre-part, from amino acid at position 32 to position 76 is the sequence for pro-part and from amino acid at position 77 to position 407 is the sequence for mature transglutaminase.

[0015] Methods for introducing the genetic constructs used in the present invention are not particularly limited, and the protoplast process (Gene, 39, 281-286 (1985); JP-Kokai No. 3-251182), electroporation process (Bio/Technology, 7, 1067-1070 (1989)) may be used. The thus obtained transformants may be cultured by using conventional methods and conditions. The medium for culturing these microorganisms may be a conventional medium, for example, a medium containing carbon sources, nitrogen sources and inorganic ions. It is preferable to add vitamins, organic micronutrients such as amino acids or natural materials such as polypeptone or yeast extract. As carbon sources, carbohydrates such as solubilized starch, glucose or sucrose, organic acids and alcohols are properly used. The culture is conducted under the aerobic condition for one day to 2 weeks appropriately maintaining pH ranging from 5.0 to 8.5 and the temperature ranging from 15° C. to 37° C.

[0016] As nitrogen sources, ammonia gas, aqueous ammonia, ammonium salts and the like are used. As inorganic ions, magnesium ion, phosphate ion, potassium ion or iron ions and the like are properly used. By culturing the transformants under these conditions, prepro-transglutaminase is largely produced in the bacterial cells and extracellularly secreted as pro-transglutaminase and then pro-transglutaminase is cleaved in the medium by a protease which is produced and secreted by a Streptomyces bacterium itself under these condition, then mature (active form) transglutaminase is largely accumulated in the medium.

[0017] Transglutaminases produced and secreted according to the present invention can be purified from the medium after culturing, depending on their properties, using the methods well known to those skilled in the art. Transglutaminase may be purified, for example, by using known appropriate techniques such as ammonium sulfate precipitation or ethanol precipitation, as well as ion-exchange column chromatography, isoelectric focusing or gel filtration or the combination thereof, after removing cells, for example, by centrifugation.

EXAMPLES

Example 1

Acquisition of Transglutaminase Gene from S. cinnamoneum IFO 12852

[0018] The sequence of transglutaminase from S. cinnamoneum CBS683.68 has been already determined (Biochimie., 80, 313-319 (1998)). The primer according to SEQ ID NO:5 and SEQ ID NO:6 are synthesized based on this sequence, and the region encoding mature transglutaminase was amplified by PCR from chromosomal DNA of S. cinnamoneum IFO 12852 prepared according to the method of Saito and Miura (Biochem., biophys., Act., 72, 619 (1963)). PCR was conducted using Pyrobest DNA polymerase (TAKARA Co.) under the condition according to its protocol.

[0019] SEQ ID NO:5 5′-TCCGATGACCGGGAAACTCCTCCCGCCGAG-3′

[0020] SEQ ID NO:6 5′-CGGCCAGCCTTGCTCCACCTTGGCGGGGGC-3′

[0021] [Free text in the sequence listing]

[0022] SEQ ID NO:5 :PCR primer for amplifying transglutaminase gene from S. cinnamoneum

[0023] SEQ ID NO:6 :PCR primer for amplifying transglutaminase gene from S. cinnamoneum

[0024] The amplified 960 bp DNA fragment are then reacted with [α-32P]dCTP using Random Primer DNA Labeling Kit Ver.2 (Takara Co.), according to the protocol attached to the kit, to generate the DNA probe. Using the generated probe and the chromosomal DNA from S. cinnamoneum IFO 12852, Southern blot hybridization was conducted according to the conventional procedures such as those described in Molecular Cloning 2nd Edition (J. Sambrook, E. F. Fritsch and T. Maniatis, Cold Spring Harbor Laboratory Press, p9.31 (1989)), which confirmed that transglutaminase gene was present in the 3.5 kb fragment which excised by BamHI restriction enzyme. The 3.5 kb BamHI digested fragment from S. cinnamoneum IFO 12852 chromosomal DNA was recovered by agarose electrophoresis using EASYTRP Ver. 2 (Takara), inserted into BamHI site of pUC18, and introduced into competent Escherichia coli JM109 cells (Takara) to generate a library. Using the previously prepared DNA probe, the library was screened by colony hybridization according to the conventional procedures such as those described in Molecular Cloning 2nd Edition (J. Sambrook, E. F. Fritsch and T. Maniatis, Cold Spring Harbor Laboratory Press, p9.31 (1989)) to obtain the strain harboring the plasmid where the fragment of transglutaminase gene was cloned. The plasmid was recovered from the strain, which was designated as pB3.5.

[0025] The sequencing of the cloned fragment in pB3.5 confirmed that the transglutaminase gene from S. cinnamoneum IFO 12852 had almost identical nucleotide sequence to the transglutaminase gene from S. cinnamoneum CBS683.68. The transglutaminase gene was inserted such that the gene was transcribed in the direction from EcoRI to HindIII site within the multi-cloning site of pUC18. The sequencing was performed using Di-terminator Cycle Sequencing Kit (PE Applied Biosystems) and DNA Sequencer 373A (PE Applied Biosystems).

[0026] The determined nucleotide sequence and the amino acid sequence encoded by the nucleotide sequence are shown in SEQ ID NO:1 and SEQ ID NO:2, respectively. It was assumed that from amino acid at position 1 to position 32 in the amino acid sequence is the sequence for pre-part, from amino acid position 33 to position 86 is the sequence for pro-part and from amino acid at position 87 to position 416 is the sequence for mature transglutaminase.

Example 2

Construction of the Expression Plasmid for Transglutaminase Gene

[0027] 1) Acquisition of plasmid vector (plJ702)

[0028] plJ702 was prepared according to [J. Bacteriol., 162, 406-412 (1985); J. Bacteriol., 169, 1929-1937 (1987)]. More specifically, Streptomyces lividans 3131 (ATCC 35287)(J. Gen. Microbiol., 129, 2703-2714 (1983)) obtained by transforming Streptomyces lividans 66 with plJ702 was cultured under the following medium condition at 30° C. for 2 days.

[YEME medium + 0.5% Glycine + 50 μg/ml thiostrepton]
0.3%  Yeast Extract
0.5%  Peptone
0.3%  Malto Extract
0.1%  Magnesium chloride
1.0%  Glucose
34.0% Sucrose
0.5%  Glycine
0.1%  50 mg/ml thiostrepton solution
      (Sigma: dimethylsulfoxide solution)(pH 7.0)

[0029] 200 ml of the broth cultured under above condition was centrifuged (12,000 g, 4° C., 10 min.), washed with 50 mM Tris-HCl (pH(8.0)—5 mM EDTA—50 mM NaCl, and the resulting bacterial cells were suspended in 10 ml of Tris-HCl (pH8.0)—10 mM EDTA—25% Sucrose ( TE-Sucrose). 2 ml of TE-Sucrose containing 30 mg/ml of Lysozyme (Sigma) and 4 ml of 0.25M EDTA was added, the mixture was incubated at 37° C. for 30 minutes, then 2 ml of 20% SDS was added. 5 ml of 5M NaCl was further added, gently mixed, and then, it was incubated overnight. After centrifugation (100,000 g, 4° C., 40 min. ), 30% polyethylene glycol 6000 was added to the resulting supernatant at the final concentration of 10% and the mixture was incubated at 0° C. for 4.5 hours. Then the mixture was centrifuged (900 g, 40° C., 5 min.) and the resulting precipitation was dissolved in 10 mM Tris-HCl (pH 8.0)—1 mM EDTA—50 mM NaCl. To the mixture 1.2 ml of the solution prepared to contain 16.8 g of cesium chloride and 10 mg/ml of ethidium bromide in 10 mM Tris-HCl(pH8.0)—1 mM EDTA (referred to as “TE”, hereinafter) was added, the residue was remove by centrifugation (1,300 g, room temperature, 15 min.), and then the mixture was centrifuged (230,000 g, 200° C., 12 hours). After centrifugation, the plasmid layer was drawn and isolated under UV lamp, the solution was repeatedly extracted with TE-saturated buthanol 3 times to remove ethidium bromide. The solution was dialyzed against TE at 4° C. overnight, extracted once with TE-saturated phenol and twice with chloroform/isoamyl alcohol, and then the aqueous layer was recovered. Then, 1/10 volume of 3M sodium acetate (pH5.2) solution and 2 volume of ethanol were added to the solution, and the mixture was allowed to stand at −80° C. for 30 minutes. The precipitation was recovered by centrifugation (12,0000 g, 4° C., 15 minutes), washed with 70% ethanol, dried and dissolved in 200 μl of TE. About 10 μg of the plasmid was obtained.

[0030] 2) Construction of the expression plasmid

[0031] A shuttle vector was firstly constructed which can replicate both in actinomycetes (Streptomyces) host and E.coli host. Multicopy plasmid plJ702 for actinomycetes was digested with SacI and PstI to prepare large 5.1 kb mel (tyrosine kinase gene) fragment from which the promoter region was deleted. pOSΔB-Ap1 (about 7.9 kb)(Appl. Environ, Microbiol., 60, 3566-3572 (1994)) into which the fusion gene of protease inhibitor SSI (Streptomyces subtilisin inhibitor) gene and microbial peptide (apidaecin) gene was digested with HindIII and PstI to prepare the fragment of about 2 kb. Multicopy plasmid pUC18 (Takara) for Escherichia coli was digested with EcoRI, blunted with T4 DNA polymerase (Takara), and self ligated. Plamids which could not be digested with EcoRI were selected and the plasmids were digested with SacI and HindIII to prepare the 2.7 kb fragment. Then, shuttle vector pUJS (about 9.8 kb) was constructed by the ternary ligation of the 5.1 kb SacI-PstI fragment from plJ702, the 2 kb HindIII-PstI fragment from pOSΔB-Ap1 and the 2.7 kb SacI-HindIII fragment from pUC1 8.

[0032] pUJS was digested with HindIII and EcoRI to recover the large 8.6 kb fragment. pB3.5 (about 6.2 kb) containing transglutaminase gene from S. cinnamoneum IFO 12852 which was cloned according to (1) was digested with HindIII and EcoRI and the 3.5 kb HindIII-EcoRI fragment was recovered. The 3.5 kb HindIII-EcoRI fragment was inserted into HindIII and EcoRI site of pUJS to construct pUJ-MTG (about 12.1 kb). The above described construction procedures are shown in FIG. 1.

[0033] E. coli AJ13669 obtained by transforming E.coli with pUJ-MTG was deposited in the National Institute of Bioscience and Human-Technology Agency of Industrial Science and Technology (1-3, Higashi 1 chome Tsukuba-shi Ibaraki-ken 305-8566, JAPAN) (The microorganism had been deposited as FERM P-17602 on Oct. 14, 1999 and was transferred to the international deposit based on the Budapest Treaty as FERM BP-7287 on Aug. 28, 2000).

Example 3

Transformation of S. lividans TK24

[0034] S. lividans TK24 is the strain derived from S. lividans 66, which is mounted a streptomycin resistance (GENETIC MANIPULATION OF STREPTOMYCES, A LABORATORY MANYAL: D. A. Hopwood et al., p266, 1985, The john Innes Foundation Norwich). This strain was provided from D. A. Hopwood (John Innes Institute, Colney Lane, Norwich NR4 7UH, U.K.) and is obtainable from D. A. Hopwood's laboratory. S. lividans Tk24 was treated to make protoplasts and transformed according to the method of [JP-Kokai No. 3-251182; Hunter, I. S., “DNA Cloning” A Practical Approach 2, Glover, D. M.(Ed.) IRL Press (1985), GENETIC MANIPULATION OF STREPTOMYCES, A LABORATORY MANUAL: D. A. Hopwood et al., p104, 1985, The John Innes Foundation Norwich]. More specifically, S. lividans was cultured in YEME medium+0.5% Glycine at 30° C. for two(2) days. 200 ml of broth was centrifuged(1,300 g, room temperature, 10 min.) and the resulting bacterial cells were suspended in 72 ml of 0.35M sucrose.

[0035] The suspension was then centrifuged (1,300 g, room temperature, 10 min.), incubated at 30° C. for 2.5 hours, filtrated with absorbent cotton to remove residues. The resulting filtrate was centrifuged (1,300 g, room temperature, 10 min.) and the precipitant was washed twice with 25 ml of P-buffer and suspended in 1 ml of P-buffer to prepare a protoplast suspension.

[0036] [P-buffer]

	TES[N-Tris(hydroxymethyl)methyl-	5.73	g
	2-aminoethane sulphonic acid]
	Sucrose	103	g
	Magnesium chloride	2.03	g
	Potassium sulfate	0.5	g
	Calcium chloride	3.68	g
	Trace elements solution	2	ml/L (pH 7.4)

[0037] 1 ml of 1% potassium phosphate solution per 100 ml of P-buffer, which had been separately prepared, was added to P-buffer immediately before use.

[0038] [Trace elements solution]

[0039] It contains the followings per 1L of the solution:

Zinc chloride       40 mg
Ferric chloride     200 mg
Cupric chloride     10 mg
Manganese chloride  10 mg
Tetra sodium borate 10 mg
ammonium molybdate  10 mg

[0040] The transformation of S. lividans TK24 protoplast suspension with pUJ-MTG (about 12.1 kb), transglutaminase gene expressing plasmid, was conducted as follows.

	DNA solution (0.2 μg/μl)	20	μl
	Protoplast suspension of S. lividans TK24	100	μl
	0.35 M Sucrose	20	μl
	P-buffer containing 20% polyethylene glycol 1000	1.5	ml

[0041] was gently mixed and allowed to stand for 2 minutes at the room temperature.

[0042] The mixture was centrifuged (1,700 g, room temperature, 10 min.) and the pellet was collected, repeatedly washed twice with P-buffer, suspended in 1 ml of P-buffer and spreaded on the following R-2 agar plates.

[0043] [R-2 Agar plate]

[0044] 1) R-2/A

	1)	R-2/A
		Potassium sulfate	0.5	g/l
		Magnesium chloride	20.2	g/l
		Calcium chloride	5.9	g/l
		Glucose	20.0	g/l
		Proline	6.0	g/l
		Casamino acid	0.2	g/l
		Trace elements solution	4	ml
		Agar	44.0	g/l
	2)	R-2/B
		TES	11.5	g/l
		Yeast Extract	10.0	g/l
		Sucrose	203	g/l (pH7.4)

[0045] 3) 1% KH2PO4

[0046] 1), 2) and 3) were separately prepared. R-2/A and R-2/B were mixed on the preparation of plates and 1 ml of 1 % KH2PO4 solution per final volume of 200ml of the mixture was added. R-2 agar plates where the transformants were plated were incubated at 30° C. for 18 hours. 1 ml of P-buffer containing 200 μg/ml of thiostrepton was poured on the pate to cover the entire surface of the agar and the plates were further incubated at 30° C. for 7 days to obtain colonies. Plasmids were prepared from the obtained colonies to confirm that the intended plasmid was introduced.

Example 4

The Expression of Transglutaminase and Secretory Production

[0047] The transformant pUJ-MTGIS.lividans TK24 was cultured in 4 ml of Tripton-Soya Broth (DIFCO) liquid medium containing 10 μg/ml of thiostrepton at 30° C. for 3 days. 1 ml of the culture was seeded into 100 ml of the same liquid medium in 500 ml Sakaguchi flask and cultured at 30° C. for 2 weeks. The samples were taken sequentially from the culture and 10 μl of the broth supernatant was subjected to SDS-PAGE and then to Western blot analysis using the anti-transglutaminase antibody described in JP-Kokai No.6-046855 according to the general method such as described in Molecular Cloning 2nd Edition (J. Sambrook, E. F. Fritsch and T. Maniatis, Cold Spring Harbor Laboratory Press, p18.60 (1989)). As a result, the secretory production (about 40-50 mg/l) of transglutaminase with additional pro-structural part was found till about day 7 to 10 of culturing and then the yield of transglutaminase having almost the same molecular weight as that of mature transglutaminase, which can be the result of processing of pro-transglutaminase, increased on further culturing. At about week 2 in culturing, about 40-50 mg/l of mature transglutaminase was accumulated.

[0048] Using the supernatant of broth at the phase where the amount of secretory production of transglutaminase with additional pro-structural part (pro-transglutaminase) was large, SDS-PAGE and semi-dry blotting to PVDF membrane were conducted ( Analysis of Protein Structure for Gene Cloning, Tokyo Kagaku Dojin (1993)). After blotting, the PVDF membrane was stained with Coomassie brilliant blue, de-stained and dried in air. The portion corresponding to pro-transglutaminase was excised and analyzed for the N-terminal amino acid sequence by the protein sequencer (Model 476A, Perkin-Elmer). The result confirmed the 10 amino acids sequence (Gly-Asp-Gly-Glu-Glu-Lys-Gly-Ser-Tyr-Ala-, SEQ ID NO:7) of pro-transglutaminase. This amino acid sequence differed from the sequence of pro-region indicated in Biochimie., 80, 313-319 (1998), but was identical to the amino acid sequence determined in Example 1 (SEQ ID NO:2).

[0049] According to the present invention, a large amount of transglutaminase can be obtained in the broth by directing Streptomyces bacteria to produce transglutaminase. Since transglutaminase accumulated in the broth according to the present invention is mature transglutaminase cleaved by proteases which are produced by the Streptomyces bacteria themselves, mature transglutaminase can be easily recovered from the broth on a large scale.

Claims

1. A bacterium belonging to Streptomyces, wherein a gene construct containing a transglutaminase gene from actinomycetes and a promoter sequence controlling the transglutaminase gene is introduced into the bacterium.

2. The bacterium belonging to Streptomyces according to claim 1, wherein the actinomycetes is Streptoverticillium cinnamoneum.

3. The bacterium belonging to Streptomyces according to claim 1 or 2, wherein the bacterium belonging to Streptomyces is Streptomyces lividans.

4. A method of producing a pro-transglutaminase derived from actinomycetes, comprising the steps of culturing the bacterium belonging to Streptomyces according to any one of claims 1 to 3, and allowing the bacterium to secrete the pro-transglutaminase into a culture medium.

5. A method of producing a mature transglutaminase derived from actinomycetes, comprising the steps of culturing the bacterium belonging to Streptomyces according to any one of claims 1 to 3, allowing the bacterium to secrete a pro-transglutaminase derived from actinomycetes into a culture medium, cleaving the pro-structural part of the pro-transglutaminase by a protease derived from said bacterium belonging to Streptomyces and recovering the mature transglutaminase.

6. The method according to claim 4, wherein the pro-transglutaminase has the amino acid sequence from Glycine residue at position 33 to Proline residue at position 416 in the sequence of SEQ ID NO:2.

7. The method according to claim 5, wherein the mature transglutaminase has the amino acid sequence from Serine residue at position 87 to Proline residue at position 416 in the sequence of SEQ ID NO:2.