Patent Grant
10751802
The disclosure of the present patent application relates to synthesis of silver nanoparticles, and particularly to methods of synthesizing silver nanoparticles using red sand, the nanoparticles having antibacterial properties.
Nanoparticles hold significant technological potential in the fields of biology, medicine and electronics owing to their unique physical and biological properties. The use naturally occurring and abundant materials for the synthesis of nanoparticles offers numerous benefits of eco-friendliness and compatibility with pharmaceutical and other biomedical applications due to the non-toxic nature of the materials involved.
Silver has very high electrical conductivity and is widely used as a conductor in circuits that require low dissipation and high conductivity. Silver paste is commonly used as a paste conductor, and particularly in conductivity characterization of bulk semiconductor materials or four-point probe method films. In the field of superconductors, silver has a dominant role as a sheath. Silver is also implicated as useful in various industries and health fields (healthcare-related products, consumer products, medical device coatings, optical sensors, cosmetics, pharmaceutical technologies, food technologies, diagnostics, orthopedics, drug delivery and antibacterial agents (particularly as an enhancer of tumor-killing effects of antibacterial drugs)). Silver has been shown to have some antibacterial properties as a catalyst.
Silver nanoparticles hold additional potential in the above-mentioned fields, particularly in biomedical fields, and particularly if they can be fabricated by methods that avoid use of expensive or toxic materials.
Red sand is an abundant resource in the area in and around Riyadh, Saudi Arabia. Although there have been attempts to use sand as at least a partial substitute for cement in recent years, currently there are no major commercial uses for red sand. Many reducing agents have been used to produce silver nanoparticles. Residual trace elements from the reducing agents may become incorporated into the nanoparticles and may affect the properties, e.g., antibacterial or antimicrobial properties, of the resulting silver nanoparticles. Thus, there is great interest in developing alternative reducing agents for producing silver nanoparticles that may be less toxic and environmentally friendly while exhibiting acceptable antibacterial activity.
Thus, a method of producing silver nanoparticles using red sand solving the aforementioned problems is desired.
A method of producing silver nanoparticles using red sand may include the steps of adding red sand to water, mixing, removing a supernatant from the red sand in water mixture, adding sodium hydroxide to the supernatant to form a solution, adding silver nitrate (AgNO3) to the solution, and isolating a reaction product that comprises the silver nanoparticles. The silver nanoparticles prepared according to the presently disclosed method are useful as antibacterial agents.
These and other features of the present disclosure will become readily apparent upon further review of the following specification and drawings.
Similar reference characters denote corresponding features consistently throughout the attached drawings.
The method of producing silver nanoparticles using red sand may include the steps of adding red sand to water, mixing the red sand in water, removing the supernatant from the red sand in water mixture, adding sodium hydroxide to the supernatant to form a solution, adding silver nitrate (AgNO3) to the solution, and isolating a reaction product that comprises the silver nanoparticles.
The step of removing a supernatant may include allowing the sand to settle and decanting the resulting supernatant, and may further include centrifuging the resulting supernatant to obtain a final supernatant. The step of adding sodium hydroxide may be performed under stirring at a temperature of about 45° C. for about 30 minutes. The step of adding silver nitrate may include dissolving silver nitrate in water and adding the silver nitrate in water dropwise into the solution. The formation of a reaction product in the solution may be confirmed by a visual change of color to brown, presumably due to surface plasmon vibrations of the silver nanoparticles formed therein.
The present method of synthesizing silver nanoparticles may provide silver nanoparticles with predictable properties and in scalable quantities. The silver nanoparticles produced by the above method may be polydispersed in size.
The method for producing silver nanoparticles can be useful in many fields. The nanoparticles are shown to have antibacterial activities, as discussed below. As red sand is an abundant resource, the present method is particularly desirable for synthesizing silver nanoparticles.
It should be understood that the amounts of materials for the methods described herein are exemplary, and appropriate scaling of the amounts is encompassed by the present method, as long as the relative ratios of materials are maintained. As used herein, the term “about,” when used to modify a numerical value, means within ten percent of that numerical value.
The term “nano”, in terms of nanomaterials, refers to materials characterized as having a dimension less than 1 micron. This is in contrast to the term “bulk” materials, which refers to macroscopic scale materials, i.e., materials having all dimensions greater than or equal to 1 micron. A “nanoparticle” is defined herein as a particle having nano-scaled dimensions in three dimensions. As used herein, the phrase “silver nanoparticles” is defined to include nanoparticles of pure silver metal, as wells as nanocomposites of pure silver metal coated or capped by elements or compounds extracted from red sand or otherwise agglomerated into nanoparticles or incorporating red sand extracts into the crystalline structure of the silver nanoparticles, as evidenced by EDX analysis.
Sand is a granular material composed of finely divided rock and mineral particles. It is defined by size, being finer than gravel and coarser than silt. Sand is typically a source of magnesium, silica (silicon dioxide, SiO2), calcium carbonate and other elements (such as Co, Ni, Sc, R, V, Cr and Ti).
The present method is illustrated by the following examples.
For the formation of exemplary silver nanoparticles according to the present method, 145.45 g of red sand, collected from the area in and near Riyadh, Saudi Arabia, was added to 100 ml of distilled water. The red sand in water was allowed to settle, and the supernatant was removed and then centrifuged at 20 rpm for about 2 min. 10 ml of sodium hydroxide (2 g) was added to 40 ml of the supernatant to form an alkaline solution and stirred at 110 rpm at a temperature of 45° C. 20 mg of silver nitrate (AgNO3) was dissolved in 20 ml of distilled water, and the silver nitrate solution was added dropwise to the alkaline solution. The reaction of silver ions from aqueous silver nitrate in the solution forming silver nanoparticles was monitored visually and deemed to have occurred upon a change of color to brown, at which point the precipitated reaction product, including the exemplary silver nanoparticles, was isolated by centrifugation and dried at 35° C.
The exemplary silver nanoparticles were characterized by dynamic light scattering (DLS) (
Transmission electron microscopy (TEM) was used to further identify the size, shape and morphology of the exemplary silver nanoparticles. The exemplary silver nanoparticles are well dispersed (not significantly aggregated) and primarily spherical in shape (
Energy dispersive x-ray analysis (EDX) confirmed the formation of silver nanoparticles and further showed the elemental composition of the exemplary silver nanoparticles.
In
Antibacterial activity of the exemplary silver nanoparticles, prepared as described above (except that centrifuging and drying were omitted, i.e., antimicrobial testing was performed without removing the silver nanoparticles from the red sand extract), was evaluated against pathogenic bacterial reference strains of Acinetobacter baumannii (ATCC 19606), Salmonella typhimurium (ATCC 14028), Escherichia coli (ATCC 35218), Pseudomonas aeruginosa (27853 AT), Staphylococcus aureus (25923 AT) and Proteus vulgaris (ATCC 49132) using an agar well diffusion assay. In particular, the antibacterial activity against each strain was determined by measuring the inhibition zone. Standard antibiotic discs, including Gentamycin (CN10 μg), Augmantin (AMC 30 μg), and Ciprofloxacin (CIP 5 μg), were used as controls.
The exemplary silver nanoparticles showed antibacterial activity against the studied most common human pathogenic bacteria with varying degrees. The activity was indicated by the diameter of inhibition zone. The red sand extract alone (i.e., prepared without addition of silver nitrate) did not show antibacterial activity. The exemplary silver nanoparticles showed the largest inhibition zone (14 mm) against the tested bacterial strain of Escherichia coli, followed by Pseudomonas aeruginosa, Salmonella typhimurium, Proteus vulgari, Acinetobacter baumannii and Staphylococcus aureus, with zones of inhibition of 13.5 mm, 13 mm, 12 mm, 11 mm and 9.5 mm, as shown in Table 1 and
S. aureus
P. vulgaris
A. baumannii
S. typhimurium
P. aeruginosa
E. colt
Moreover, combination effects were determined by first adjusting the turbidity of the previously mentioned bacterial strains to 0.5 MacFarland standards (108 CFU/mL), and swabbing the strains on Mueller-Hinton agar. Antibiotic discs alone were used as controls, respectively. In particular, the antibiotic discs had standard amounts of Fosfomycin (FOS) (50 μg), Tetracycline (TE) (30 μg), Cefepime (FEP) (30 μg), Moxifloxacin (MXF) (5 μg), Levofloxacin (LEV) (5 μg), Rifampicin (RD) (5 μg), Erythromycin (E) (15 μg), Tobramycin (TOB) (10 μg), and Tigecycline (TGC) (15 μg), respectively. To study the combination effect, 30 μl of the exemplary silver nanoparticles were loaded on the antibiotics discs then placed on the swabbed medium. The plates were incubated for 24 hours at 37° C. The diameters of the inhibition zones were measured and reported in millimeters.
The greatest combination effects of the exemplary silver nanoparticles with antibiotics occurred on Salmonella typhimurium, as shown in Table 2. Relative to the results shown in Table 1 showing the effect of the exemplary silver nanoparticles on S. typhimurium to be an inhibition zone with diameter 13 mm, the exemplary silver nanoparticles combined with the Fosfomycin (FOS) 50 μg standard resulted in an inhibition zone diameter increased to 25 mm. Overall, the Moxifloxacin (MXF) 5 μg displayed the strongest effect on the tested g-negative bacteria.
S. typhimurium
E. coli
A. baumannii
P. aeruginosa
P. vulgaris
S. aureus
It is to be understood that the method of producing silver nanoparticles using red sand is not limited to the embodiments described above, but encompasses any and all embodiments within the scope of the following claims.
| Number | Name | Date | Kind |
|---|---|---|---|
| 20120308666 | Zielecka | Dec 2012 | A1 |
| 20130108678 | Santra | May 2013 | A1 |
| Number | Date | Country |
|---|---|---|
| 103642355 | Mar 2014 | CN |
| 100787544 | Dec 2007 | KR |
| Entry |
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| Shameli et al., “Synthesis of silver nanoparticles in montmorillonite and their antibacterial behavior”, International Journal of Nanomedicine (2011), vol. 6, pp. 581-590. |
