Claims
- 1. A method for the quantitative determination of a target material in a sample comprising the steps of:
- (i) reacting in solution phase (a) the target material and (b) a known amount of a peroxidase-labeled receptor having a specific binding activity to the target material to form aggregates of the two components (a) and (b);
- (ii) effecting an enzymatic reaction between the peroxidase contained in the aggregates, a peroxide, a phenol hydrogen donor, and a coupler for said phenol, the peroxide being present in an amount sufficient to inhibit the enzyme in non-aggregated peroxidase labeled receptor to produce an analytically detectable change; and
- (iii) quantitatively determining the target material from the analytically detectable change.
- 2. A method according to claim 1 wherein the peroxide is in the amount of about 34 mM.
- 3. A method for the quantitative determination of a target material in a sample comprising the steps of:
- (i) reacting in solution phase (a) the target material, (c) a known amount of a receptor having a specific binding activity to the target material, and (d) a known amount of a peroxidase-labeled target material, to form aggregates of the three components (a), (c), and (d);
- (ii) effecting an enzymatic reaction between the peroxidase contained in the aggregates, a peroxide, a phenol hydrogen donor, and a coupler for said phenol, the peroxide being present in an amount sufficient to inhibit the enzyme in non-aggreated peroxidase-labelled target material to produce an analytically detectable change; and
- (iii) quantitatively determining the target material from the analytically detectable change.
- 4. A method according to claim 3 wherein the peroxide is in the amount of about 34 mM.
- 5. A method for the quantitative determination of a target material in a sample comprising the steps of:
- (i) reacting in solution phase (a) the target material, (e) a known amount of a peroxidase, and (f) a receptor having specific binding activities to both the target material and the peroxidase to form aggregates of the components (a), (e), and (f);
- (ii) effecting an enzymatic reaction between the peroxidase contained in the aggregates, a peroxide, a phenol hydrogen donor, and a coupler for said phenol, the peroxide being present in an amount sufficient to inhibit non-aggregated peroxidase to produce an analytically detectable change; and
- (iii) quantitatively determining the target material from the analytically detectable change.
- 6. A method according to claim 5 wherein the peroxide is in the amount of about 34 mM.
Priority Claims (1)
Number |
Date |
Country |
Kind |
57-3314 |
Jan 1982 |
JPX |
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CROSS REFERENCE TO RELATED APPLICATIONS
This application is a continuation of application Ser. No. 724,020, filed Apr. 17, 1985 and now abandoned, which is a continuation of application Ser. No. 452,435, filed Dec. 23, 1982 and now abandoned.
US Referenced Citations (10)
Non-Patent Literature Citations (1)
Entry |
Annals of Clinical Biochemistry 16, 221-239 (1979) M. J. O'Sullivan et al. "Enzyme immunoassay: a review". |
Continuations (2)
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Number |
Date |
Country |
Parent |
724020 |
Apr 1985 |
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Parent |
452435 |
Dec 1982 |
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