Claims
- 1. A method of separating a compound of interest from a mixture, the method comprising the steps of:
(a) providing a mixture containing a compound of interest; (b) subjecting a portion of the mixture to a separation using thin layer chromatography to determine an Rf value for the compound of interest; (c) predicting an elution time of the compound of interest on a preparative scale HPLC column using the determined Rf value for the compound of interest; (d) subjecting all or a portion of the remaining mixture to a preparative scale HPLC system comprising a preparative scale HPLC column; and (e) collecting at least a portion of the compound of interest using the predicted elution time.
- 2. The method of claim 1 wherein the step of predicting an elution time for the compound of interest comprises:
(1) predicting a retention time of the compound of interest from the preparative scale HPLC column using a predetermined correlation function between Rf value from the TLC and retention time on the preparative scale HPLC column along with the determined Rf value of the compound of interest; and (2) selecting a window of time around the predicted retention time within which the compound is expected to elute.
- 3. The method of claim 1 wherein step (b) comprises:
(1) subjecting a portion of the mixture to a separation using thin layer chromatography to produce one or more spots or zones; (2) analyzing the one or more spots or zones using a mass spectrometer to determine the spot or zone containing the compound of interest; and (3) determining an Rf value for the compound of interest.
- 4. The method of claim 1 wherein an artificial neural network is used to predict the elution time in step (c).
- 5. The method of claim 1 wherein a dynamic correlation function is used to predict the elution time in step (c).
CROSS REFERENCE TO RELATED APPLICATION
[0001] This application is a divisional of U.S. patent application Ser. No. 09/998,137, filed Dec. 3, 2001, the entire content of which is incorporated herein by reference.
Divisions (1)
|
Number |
Date |
Country |
Parent |
09998137 |
Dec 2001 |
US |
Child |
10790436 |
Mar 2004 |
US |