Claims
- 1. A method of sterilizing an object, comprising the steps of:
contacting the object with a composition of a branched polycation at a pH of 5.0 or less; and allowing the composition to remain in contact with the object for a period of time sufficient to render a conformationally altered protein non-infectious.
- 2. The method of claim 1, further comprising:
removing the composition from the object.
- 3. The method of claim 1, wherein the branched polycation is a polycationic dendrimer selected from the group consisting of polypropylene imine, polyethyleneimine (PEI) poly(4′-aza-4′-methylheptamethylene D-glucaramide), polyamidoamines and pharmaceutically effective variants or fragments thereof,
wherein the pH is maintained at 4.5 or less; and wherein the conformationally altered protein is selected from the group consisting of APP, Aβ peptide, α-1-antichymotrypsin, non-Aβ component, presenillin 1, presenillin 2, apoE and PrPSc.
- 4. The method of claim 1, wherein the object is a medical device.
- 5. The method composition of claim 1, wherein the object is a surgical instrument.
- 6. The method of claim 1, wherein the object is a catheter.
- 7. The method of claim 1, wherein the object is an operating room.
- 8. The method of claim 1, wherein the object is a cell culture.
- 9. The method of claim 1, wherein the object is gelatin material obtained from a cow.
- 10. The method of claim 1, wherein the branched polycation is a polycationic dendrimer selected from the group consisting of polypropylene imine, polyethyleneimine (PEI) poly(4′-aza-4′-methylheptamethylene D-glucaramide), polyamidoamines and variants or fragments thereof.
- 11. A composition, comprising:
water in an amount of from 1% to 99.99% by weight; polycationic dendrimer in an amount of 0.001% to 10% by weight.
- 12. The composition of claim 11, further comprising:
detergent in an amount of about 1% to 20% by weight.
- 13. The composition of claim 12, further comprising:
an antibacterial compound in an amount of about 0.1% to 20% by weight.
- 14. The composition of claim 13, further comprising:
an antiviral compound and an antifungal compound each in an amount of 0.1% to 20% by weight.
- 15. A capsule for oral administration of a compound, comprising:
gelatin extracted from a cow; and a branched polycation.
- 16. The capsule of claim 15, further comprising:
a pharmaceutically active drug in the capsule.
- 17. The capsule of claim 15, further comprising:
a vitamin in the capsule.
- 18. The capsule of claim 15, wherein the branched polycation is a polycationic dendrimer selected from the group consisting of polypropylene imine, polyethyleneimine (PEI) poly(4′-aza-4′-methylheptamethylene D-glucaramide), polyamidoamines and pharmaceutically effective variants or fragments thereof;
wherein the branched polycation is combined with the gelatin in an amount in a range of from about 0.001 mg to 1 mg of polycation per kilogram of gelatin.
- 19. A cell culture, comprising:
cells genetically engineering to produce a pharmaceutical; cell nutrients; and a branched polycation.
- 20. The cell culture of claim 19, wherein the branched polycation is a polycationic dendrimer selected from the group consisting of polypropylene imine, polyethyleneimine (PEI) poly(4′-aza-4′-methylheptamethylene D-glucaramide), polyamidoamines and pharmaceutically effective variants or fragments thereof.
CROSS-REFERENCES
[0001] This application is a continuation-in-part of earlier filed application Ser. No. 09/447,456 filed Nov. 22, 1999, which is a continuation-in-part of application Ser. No. 09/322,903 filed Jun. 1, 1999 which applications are incorporated herein in their entirety and to which application is claimed priority under 35 U.S.C. §120.
GOVERNMENT SUPPORT
[0002] This work was supported, in part, by grants from the National Institutes of Health NS14069, AG08967, AG02132, AG10770 and K08 NS02048-02. The government may have certain rights in this work.
Continuations (1)
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Number |
Date |
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Parent |
09494814 |
Jan 2000 |
US |
Child |
09956705 |
Sep 2001 |
US |
Continuation in Parts (2)
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Number |
Date |
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Parent |
09447456 |
Nov 1999 |
US |
Child |
09494814 |
Jan 2000 |
US |
Parent |
09322903 |
Jun 1999 |
US |
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09447456 |
Nov 1999 |
US |