Claims
- 1. In a biological or chemical assay, a method of transferring a quantity of liquid having a volume of less than about 2 μl comprising the steps of:(a) providing a transfer tool having at least one rod of predetermined diameter having a hydrophilic bottom surface, which is wetting and at least one oleophobicside surface, which is non-wetting. (b) immersing said rod into a reservoir containing a liquid for a predetermined period of time and at a predetermined depth, (c) removing said rod from said reservoir such that a liquid drop is retained on said bottom surface of said rod, (d) locating said rod above a receiving medium, and (e) contacting said drop with a surface of a receiving medium.
- 2. The method of claim 1, wherein said receiving medium is a multiwell plate having a plurality of wells.
- 3. The method of claim 2 wherein said drop is contacted with an interior surface of a well in said multiwell plate.
- 4. The method of claim 3 wherein said interior surface of said well is wetting.
- 5. The method of claim 1, wherein said transfer tool has a plurality of rods arranged and separated so as to align with a distribution of wells of a multiwell plate.
- 6. The method of claim 1, wherein said depth is at least twice said diameter of said rod.
- 7. The method of claim 1, wherein said length of immersion is a period of time sufficient to allow said liquid to achieve a meta-stable equilibrium configuration on said bottom surface.
- 8. A method of transferring a liquid drop from a well of a multiwell plate comprising the steps of:(a) providing a transfer tool having at least one rod having a hydrophilic bottom surface, which is wetting and at least one oleophobic side surface, which is non-wetting. (b) immersing said rod in said well containing liquid for a predetermined time at a predetermined depth, (c) removing said rod from said well such that a liquid drop is retained on said bottom surface of said rod, (d) locating said rod above a receiving container, and (e) contacting said drop with said receiving container.
- 9. The method of claim 8, wherein said transfer tool has a plurality of rods arranged and separated so as to align with a distribution of wells of a multiwell plate.
- 10. The method of claim 8, wherein said depth is at least twice said diameter of said rod.
- 11. The method of claim 8, wherein said length of immersion is a period of time sufficient to allow said liquid to achieve a meta-stable equilibrium configuration on said bottom surface.
- 12. The method of claim 8, wherein said interior surface of said well is wetting.
- 13. a biological or chemical assay, a method of transferring a quantity of liquid having a volume of less than about 2 μl comprising the steps of:(a) providing a transfer tool having at least one rod having a radius ranging from approximately 2 mm-1 μm and having a wetting tip and at least one non-wetting side, said at least one rod having a diameter less than or equal to the capillary length of said liquid and composed of either a glass, a ceramic, or a polymer material, and a support structure holding said at least one rod; (b) immersing said at least one rod into a reservoir containing a liquid for a predetermined period of time and at a predetermined depth; (c) removing said at least one rod from said reservoir such that a liquid drop is retained on a bottom surface of said tip of said at least one rod; (d) locating said at least one rod above a receiving medium; and, (e) contacting said drop with a surface of said receiving medium.
- 14. In a biological or chemical assay, a method of transferring a quantity of liquid having a volume of less than about 2 μl comprising the steps of:(a) providing a transfer tool having at least one rod having a radius ranging from approximately 2 mm-1 μm and having a wetting tip and at least one non-wetting side, said at least one rod having a diameter less than or equal to the capillary length of said liquid and composed of either a glass, a ceramic, or a polymer material, and a support structure holding said at least one rod; (b) immersing said at least one rod into a reservoir containing a liquid for a predetermined period of time and at a predetermined depth; (c) removing said at least one rod from said reservoir such that a liquid drop is retained on a bottom surface of said tip of said at least one rod; (d) locating said at least one rod above a receiving container; and, (e) contacting said drop with a surface of said receiving container.
Priority Claims (1)
Number |
Date |
Country |
Kind |
92 07466 |
Jun 1997 |
FR |
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Parent Case Info
This is a division of application Ser. No. 09/059,667, filed Apr. 13 1998, and now U.S. Pat. No. 6,051,190 which claimed the benefit of provisional application No. 60/053,971 filed Jul. 28, 1997 the benefit of which is hereby claimed herein.
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Provisional Applications (1)
|
Number |
Date |
Country |
|
60/053971 |
Jul 1997 |
US |