Method of treatment of aggression

Abstract

The invention comprises a method of treatment of aggression and similar behavioral syndromes, such as impulsivity and irritability, by a pharmaceutical agent exhibiting combined D2 and D5 antagonistic activity.

Description

BACKGROUND

Aggression and similar syndromes, including impulsivity and irritability, represent a broad category of behaviors that complicate the management of several disease states, such as attention deficit hyperactivity disorder (ADHD), bipolar disorder, autism, and post traumatic stress disorder. In some cases, 25-50% of patients optimally treated for the underlying disorder continue to manifest these syndromes (J Am Acad Child Adolesc Psychiatry. 2007 March; 46(3):309-22.).

Dopaminergic therapies are among the most prescribed for these behavioral syndromes, and include such molecules as haloperidol and other antipsychotics. The dopamine receptors for these molecules are grouped into 2 families: the D1, which includes the D1 and D5 receptor, and the D2, which includes the D2, D3 and D4 receptors. The two families differ by the manner in which the receptor protein is incorporated into the cell membrane, and by the pharmacology of the molecules that have an affinity for each type. Each receptor type is a distinct entity with its unique gene, anatomy in the brain and affinity for different molecules. Some dopamine receptor subtypes, such as the D2 receptor, have further modifications in the protein structure, giving rise to further subclassification, e.g., D2_short and D2_long.

There is increasing evidence that D5 receptor activity would be beneficial in the treatment of aggression and similar behavioral syndromes.

The D5 receptor has very specific localization in the brain, and is found in such areas as the Parafascicular Nucleus of the Thalamus, as well as the prefrontal cortex, hippocampus, ventral tegmental area, Substantia Nigra and Raphe nucleus (Hartman D S, Civelli O. Molecular attributes of dopamine receptors: new potential for antipsychotic drug development. Ann Med 1996; 28(3):211-9). The Parafascicular nucleus is involved in the behavioral process of attention to critical sensory input and activation of the subject toward that stimulus. One of the important paradigms in which the Parafascicular nucleus participates is the activation of the fight or flight response. Therefore, the Parafascicular nucleus is likely involved in activating early components of aggressive behavior (Matsumoto N, Minamimoto T, Graybiel A M, Kimura M. Neurons in the thalamic CM-Pf complex supply striatal neurons with information about behaviorally significant sensory events. J Neurophysiol 2001; 85(2):960-76).

The gene for the D5, DRD5, is associated with impulsiveness and with symptomology associated with disruptive behavioral disorders, such as antisocial personality disorder (Vanyukov M M, Moss H B, Kaplan B B, Kirillova G P, Tarter R E. Antisociality, substance dependence, and the DRD5 gene: a preliminary study. Am J Med Genet 2000; 96(5):654-8). DRD5 is also associated with genetic transmission of a number of disorders associated with aggression, irritability and impulsivity, including schizophrenia, Tourette's and ADHD (Maher B S, Marazita M L, Ferrell R E, Vanyukov M M. Dopamine system genes and attention deficit hyperactivity disorder: a meta-analysis. Psychiatr Genet 2002; 12(4):207-15). Blockade of the D5 receptor in a knockout model is associated with decreased motor activity, which may be akin to decreased aggression (Holmes A, Hollon T R, Gleason T C, et al. Behavioral characterization of dopamine D5 receptor null mutant mice. Behav Neurosci 2001; 115(5):1129-44).

Molindone is a typical antipsychotic drug that has high affinity for D2 family of dopamine receptors. where it is thought to exert its therapeutic action. Molindone was previously suggested for the treatment of aggression in both adult and pediatric patients (Greenhill L L, Barmack J E, Spalten D, Anderson M, Halpern F. Molindone Hydrochloride in the treatment of aggressive, hospitalized children [proceedings]. Psychopharmacol Bull 1981; 17(1):125-7; Itil T M, Wadud A. Treatment of human aggression with major tranquilizers, antidepressants, and newer psychotropic drugs. J Nerv Ment Dis 1975; 160(2-1):83-99). Molindone was also evaluated for children with the early-onset schizophrenia spectrum disorders (J Am Acad Child Adolesc Psychiatry, 2007, August, 46:8, p. 969-978 and Am J Psychiatry, 165:11, November 2008).

For adults with schizophrenia, the dose of molindone may range from 100 to 225 mg per day (Bagnall A, Fenton M, Kleijnen J, Lewis R. Molindone for schizophrenia and severe mental illness. Cochrane Database Syst Rev 2007 (1):CD002083). In general, the dose of other antipsychotics used for the treatment of aggressive behavior are about 50% relative to those used for the treatment of psychosis in schizophrenia (J Am Acad Child Adolesc Psychiatry. 2006 July; 45(7):792-800).

SUMMARY OF THE INVENTION

The current invention offers a method of treatment of aggression and/or similar behavioral syndromes, including irritability and impulsivity that comprises the steps of: 1. selecting several active agents with known activity (e.g., agonist or antagonist) on at least one dopaminergic, serotonergic or gabaergic receptor where the opposite activity is associated with aggressive behavior; 2. conducting a receptor screening assay on these same receptor families with each of the active agents to identify activity for at least one additional receptor having a known association with undesired (i.e., aggressive) behavior; 3. determining if said activity is agonistic or antagonistic in nature; 4. by the results of steps 2 and 3, choosing among the screened active agents at least one that targets the most diverse types of aggression-associated receptors; 5. optimizing the total dose of the active agent(s), taking into account results of steps 2 through 4; and 6. administering the dose of step (5) to a mammalian subject.

In one embodiment of the invention, the mammalian subject is a human child. In another embodiment of the invention, the mammalian subject is an adult human.

Identification of agonist/antagonist activity in the active agents that exhibit the potential as therapeutics for aggression and similar behavioral syndromes is important, since it allows for selection of drugs that have more than one therapeutic target (e.g., both D2 and D5 receptors). This is superior to taking a combination of therapies to achieve multiple targets because of the enhanced patient compliance with the reduced pill load. It also can lead to a lower dose, since different receptor activities may be additive, or even synergistic, in their effect.

Use of molecules that target a specific class of receptors with a limited distribution in the brain is also potentially beneficial in that it limits the potential for side effects. This restricted set of neural pathways is less likely to have “off-target” effect in the systems not involved in the desired activity.

In one embodiment, the invention comprises a method of treatment of aggression and similar behavioral syndromes, such as impulsivity and irritability in a mammalian subject, by a pharmaceutical agent exhibiting combined D2 and D5 antagonistic activity.

In another embodiment, the invention discloses a method of treatment of aggression and similar behavioral syndromes by a pharmaceutical agent exhibiting combined D2 and D5 antagonistic activity, wherein the total dosage of the pharmaceutical agent is smaller than the dosage anticipated on the premise of D2 activity only.

In yet another embodiment of the invention, the pharmaceutical agent is molindone.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1 shows a competition curve obtained with compound SPN-810M (molindone) at the human D5 receptor.

FIG. 2 shows the agonist effect of compound SPN-810M at the Human D5 Receptor.

FIG. 3 shows the antagonist effect of compound SPN-810M at the Human D5 receptor.

FIG. 4 shows a reduction in Conduct Problem Subscale Score achieved through administration of molindone in a randomized, multicenter, parallel group dose-ranging study in children with ADHD and persistent serious conduct problems.

FIG. 5 shows an adverse effect profile of molindone in a randomized, multicenter, parallel group dose-ranging study in children with ADHD and persistent serious conduct problems

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

It was unexpectedly discovered that, in addition to the well-documented D2-receptor group antagonistic activity, molindone has specific antagonist activity at the D5 dopaminergic receptor.

A heterologous competition assay was designed for a receptor affinity screening of molindone. Recombinant D5 receptors were expressed in a GH4 cell line and specific binding was characterized by displacement of another molecule with affinity for the D5 receptor (the “ligand”). “Specific binding” refers here to the difference in the binding of the ligand to the receptors in the presence and absence of an excess of the active agent. An initial screen was performed with 10⁻⁵ μM molindone. The conditions and results of the assay are summarized in the Tables 1 and 2.

TABLE 1
Conditions of the displacement assay for molindone
					Method of
Assay	Ligand	Conc.	Non-specific	Incubation	detection
D1(h)	[3H]SCH	0.3	SCH 23390*	60 min./	Scintillation
	23390	nM	(1 μM)	22 °C.	counting
D2S(h)	[3H]spiperone	0.3	(+)butaclamol	60 min./	Scintillation
		nM	(10 μM)	22 °C.	counting
D3(h)	[3H]spiperone	0.3	(+)butaclamol	60 min./	Scintillation
		nM	(10 μM)	22 °C.	counting
D5(h)	[3H]SCH	0.3	SCH 23390	60 min./	Scintillation
	23390	nM	(10 μM)	22 °C.	counting
*SCH 23390 is a highly potent and selective dopamine D1-like receptor antagonist with a K(i) of 0.2 and 0.3 nM for the D1 and D5 dopamine receptor subtypes, respectively.

TABLE 2
Results of the displacement assay for molindone
(concentration 1.0E−05M)
Assay % Inhibition of control specific binding
D1    55
D2s   98
D3    100
D4.4  38
D5    59

The affinity of molindone for the D5 receptor was further characterized by determining the IC50, or concentration that inhibits 50% of control specific binding. For this experiment, a range of molindone concentrations was selected for the ligand blocking assay. The IC50 was determined using non-linear regression analysis of the competition curves using Hill equation curve fitting. The inhibition constants Ki, wherein Ki is defined as the concentration of the competing ligand (molindone) that will bind to half the binding sites at equilibrium in the absence of radioligand or other competitors, were calculated using Cheng Prusoff equation. The results of the affinity assay are summarized in Tables 3 and 4, and in FIG. 1.

TABLE 3
IC50 Determination: Individual Data
	Test	% of Control Specific Binding
Receptor	Concentration(M)	1st	2nd	Mean
D1(h)	3.0E−08	105.5	95.5	102.0
	1.0E−07	95.6	96.5	96.0
	3.0E−07	93.4	92.6	93.0
	1.0E−06	90.9	70.3	80.6
	3.0E−06	52.2	56.8	54.5
	1.0E−05	31.6	30.7	31.1
	3.0E−05	18.4	12.6	15.5
	1.0E−04	2.8	3.7	3.2
D2S(h)	3.0E−10	99.1	95.6	97.3
	3.0E−09	95.8	104.6	100.2
	1.0E−08	91.0	94.7	92.8
	3.0E−08	68.0	71.9	69.9
	1.0E−07	22.0	28.1	25.1
	3.0E−07	4.4	1.9	3.1
	1.0E−06	0.1	−0.7	−0.3
	1.0E−05	−1.7	−3.0	−2.4
D3(h)	3.0E−10	98.4	100.0	99.2
	3.0E−09	103.7	102.6	103.2
	1.0E−08	101.9	107.3	104.6
	3.0E−08	99.3	90.6	94.9
	1.0E−07	71.4	63.4	67.4
	3.0E−07	31.4	32.3	31.9
	1.0E−06	8.1	9.1	8.6
	1.0E−05	−1.0	0.9	−0.1
D5(h)	3.0E−08		123.6	115.3
	1.0E−07	106.9	130.3	120.8
	3.0E−07	111.2	132.3	120.9
	1.0E−06	109.6	118.9	104.7
	3.0E−06	90.5	78.1	80.2
	1.0E−05	82.2	43.4	34.9
	3.0E−05	26.3	18.3	18.5
	1.0E−04	18.6	8.3	6.8

TABLE 4
IC50 Determination for Molindone: Summary Results
	Reference
Assay	compound	IC50 (M)	K1 (M)	n(H)
D1	SCH 23390	3.9E−06	1.5E−06	0.9
D2s	(+) butaclamol	5.1E−08	1.7E−08	1.7
D3	(+) butaclamol	1.6E−07	3.6E−08	1.4
D5	SCH 23390	5.2E−06	2.4E−06	1.2

The final step of the assay was to characterize whether the binding is of an agonist or antagonist nature. For the D5 receptor, this was accomplished through an assay that examined the agonist effect on the D5 receptor, i.e., the generation of cAMP or the blockade of this effect when stimulated by a D5 agonist, dopamine. This was also done with a range of concentrations to determine the relative agonist vs antagonist binding Ki. The EC₅₀ values (concentration producing a half-maximal specific response) and IC₅₀ values (a concentration causing a half-maximal inhibition of the control-specific agonist response) were determined by non-linear regression analysis of the concentration-response curves generated with mean replicate values using Hill equation curve fitting. The apparent dissociation constants for antagonists K_b were calculated using the modified Cheng Prusoff equation.

The conditions of the screening are presented in Table 5. It was found that molindone (10⁻⁵ μM) exhibited 59% inhibition of the SCH23390 binding at the D5 receptor. The Ki was determined to be 2.4×10⁻⁶. The binding was determined to be that of an antagonist. The full results of the screening are summarized in Tables 6-8 and in FIG. 2-3.

TABLE 5
Agonist/Antagonist screening Assay: Conditions
		Incu-	Reaction	Method of
Assay	Stimulus	bation	Product	Detection
D1(h)	None (10 μM	30 min./	cAMP	HTRF
(agonist effect)	dopamine for	22° C.		(Homogeneous
	control)			Time Resolved
				Fluorescence)
D1(h)	Dopamine	30 min./	cAMP	HTRF
(antagonist	(1000 nM)	22° C.
effect)
D2S(h)	None (100 nM	20 min./	cAMP	HTRF
(agonist	dopamine for	37° C.
effect)	control)
D2s(h)	Dopamine (30	20 min./	cAMP	HTRF
(antagonist	nM dopamine	37° C.
effect)	for control)
D3(h)	None (30 nM	10 min./	cAMP	HTRF
(agonist effect)	dopamine for	37° C.
	control)
D3(h)	dopamine (10	10 min./	cAMP	HTRF
(antagonist	nM)	37° C.
effect)
D5(h)	None (10 μM	30 min./	cAMP	HTRF
(agonist effect)	dopamine for	22° C.
	control)
D5(h)	Dopamine (100	30 min./	cAMP	HTRF
(antagonist	nM)	22° C.
effect)

TABLE 6
IC50 Determination for D5 (h): Individual Data
(Antagonist Effect)
	Agonist Response
Test Concen -	% of	% of	% of Control,
tration (M)	Control, 1st	Control, 2nd	Mean
1.0E−07	107	115	111.0
3.0E−07	90	95	92.9
1.0E−06	98	95	96.4
3.0E−06	67	66	66.7
1.0E−05	40	36	38.4
2.0E−05	19	20	19.6
5.0E−05	4	11	7.3
1.0E−04	7	4	5.6

TABLE 7
IC50 Determination: Summary Results (Antagonist Effect)
Receptor	IC50 (M)	KB (M)
D1(h)	3.1E−05	2.0E−06
D2S(h)	6.8E−08	4.7E−09
D3(h)	1.1E−06	7.1E−08
D5(h)	5.3E−06	9.2E−07

TABLE 8
EC50 Determination for D5 (h): Individual Data (Agonist Effect)
Test	Agonist Response
Concentration	% of	% of	% of
(M)	Control, 1st	Control, 2nd	Control, Mean
1.0E−07	2	2	2.1
3.0E−07	4	−1	1.8
1.0E−06	0	0	0.1
3.0E−06	3	4	3.3
1.0E−05	−1	2	0.6
2.0E−05	−2	2	0.2
5.0E−05	1	−2	−0.7
1.0E−04	1	4	2.1

Based on the receptor findings, it was suggested that molindone may be effective for the treatment of aggression in humans, in particular, in children with ADHD, in smaller doses than is currently customary. A lower daily dose of the drug results in a diminishing frequency and severity of the adverse effects of the treatment to a level of tolerability, thus resulting in improvement in patient compliance.

In one embodiment, the invention is directed to a method of treatment of aggression in humans, in particular children, diagnosed with ADHD, bipolar disorder, autism, and post traumatic stress disorder by administering molindone in a total daily dose that is at least 10% lower than the current dose for its use as an antipsychotic agent. In other embodiments, the dose is 15% lower, 25% lower, 35% lower, and 50% lower than the current dose.

In one embodiment of the invention, molindone is administered in a dose range of from 10 mg/day to 200 mg/day. In other embodiments, molindone is administered in dose ranges of from 3 mg/day to 200 mg/day; 15 mg/day to 120 mg/day; 15 mg/day to 90 mg/day; 30 mg/day to 90 mg/day; or 36 mg/day to 72 mg/day.

The success of the treatment is evident in the marked decrease in the number instances of undesirable behavior exhibiting aggression, irritability and impulsivity. Such behavior may be exemplified, without limitation, by disobedience; negativism; provocative opposition to authority figures; violations of minor rules; temper tantrums; argumentativeness; provocative behavior; stubbornness; blaming others for his or her own mistakes; being touchy, easily annoyed, angry, resentful, spiteful or vindictive; and swearing.

In another embodiment, the invention encompasses a method of treatment of aggression in children with ADHD with molindone that is characterized by an improved adverse effect profile. The adverse effects that are diminished by the method of the present invention may be, but are not limited to, drowsiness, depression, hyperactivity and euphoria, extrapyramidal reactions, akathisia, akinesia, dystonic syndrome, tardive dyskinesia, tachycardia, nausea, dry mouth, urinary retention, and constipation.

The efficacy and the adverse effect profile of the lower dose treatment of the current invention were evaluated in a Randomized, Multicenter, Parallel Group, Dose Ranging Study to Evaluate the Safety and Tolerability of molindone in Children with Attention-Deficit-Hyperactivity Disorder (ADHD) and Persistent Serious Conduct Problems, where the subjects are administered molindone hydrochloride given 3 times daily in a dose range of 5 to 20 mg/day in children <30 kg, and 10 to 40 mg/day in children ≥30 kg (Example 1 and FIG. 4).

Example 1

Effectiveness, safety and tolerability of molindone was evaluated in a randomized, multicenter, parallel group dose-ranging study in children with ADHD and persistent serious conduct problems. Subjects were randomized based on weight at baseline to 1 of 4 treatment groups. The dosing for subjects with weight of less than 30 kg was initiated at 1.67 mg/day and 3.34 mg/day for subjects weighing 30 kg or more. A titration schedule was followed until the target dose (Treatment group 1, 2, 3 or 4) was reached. Subjects remained at target dose for 6 weeks (Table 9).

TABLE 9
Total daily dose by treatment group
		Treatment	Treatment	Treatment	Treatment
		group 1	group 2	group 3	group 4
	<30 kg	5 mg/day	10 mg/day	15 mg/day	20 mg/day
	≥30 kg	10 mg/day	20 mg/day	30 mg/day	40 mg/day

The study showed a statistically significant efficacy signal towards reducing Conduct Problem Subscale scores. (Table 10; FIG. 4).

TABLE 10
Mean Reduction from Baseline in NCBRF-TIQ: Conduct
Problem Subscale Score
	Treatment 1	Treatment 2	Treatment 3	Treatment 4
Baseline Mean	20.35	25.74	26.00	25.95
Visit 12 Mean	13.40	17.00	17.79	11.65
Mean Reduction	6.95	8.74	8.21	14.30

Side effects from the administration of molindone in all four treatment groups were mild, self-limiting and within the limits of tolerability, i.e. no subjects discontinued the study because of the side effects (FIG. 5).

Claims

1. A method of treating impulsive aggression in a post-traumatic stress disorder (PTSD) patient, comprising: administering 10 mg/day to 200 mg/day of molindone to a patient with PTSD as the sole condition and manifesting impulsive aggression not controlled by PTSD treatment.

2. The method of claim 1, wherein 15 to 120 mg/day of molindone is administered.

3. The method of claim 1, wherein the administration decreases side effects associated with molindone.

4. The method of claim 3, wherein the side effects are selected from drowsiness, depression, hyperactivity, euphoria, extrapyramidal reactions, akathisia, akinesia, dystonic syndrome, tardive dyskinesia, tachycardia, nausea, dry mouth, urinary retention, and constipation.

5. The method of claim 1, wherein the administration results in reduction of undesirable behavior and intolerable side effects.

6. The method of claim 1, wherein the patient is a child or an adult.