Claims
- 1. An article of manufacture comprising:
from 7 to 300 micrograms of synthetic L-Se-methylselenocysteine; and a non-toxic, pharmaceutically acceptable binder.
- 2. The article of claim 1 wherein said binder is selected from the group consisting of vitamins, minerals, herbals and starch.
- 3. The article of claim 1 wherein said binder is selected from the group consisting of calcium carbonate, magnesium hydroxide, magnesium sulfate, sodium tetraborate, cupric oxide, zinc sulfate, cholecalciferol, fumarate, pyridoxine hydrochloride, chlorine picolinate, folate, calcium phosphate and their salts.
- 4. A method of preventing or reducing the risk of developing cancer in mammals comprising administering a nutriceutical amount of synthetic L-Se-methylselenocysteine in the range of 7 to 300 micrograms/day.
- 5. The method of claim 4 wherein said mammals are humans.
- 6. The method of claim 4 wherein said nutriceutical amount is in the range of 7 to 200 micrograms/day.
- 7. The method of claim 6 wherein said nutriceutical amount is in the range of 7 to 100 micrograms/day.
- 8. The method of claim 4 wherein said nutriceutical amount is in the range of 50 to 200 micrograms/day.
- 9. The method of claim 4 wherein said nutriceutical amount is in the range of 200 to 300 micrograms/day.
- 10. The method of claim 4 wherein said mammals are selected from the group consisting of cats and dogs.
- 11. The method of claim 10 wherein said nutriceutical amount is in the range 1-5 micrograms of selenium/kg of body weight/day.
- 12. The method of claim 10 wherein said nutriceutical amount is in the range 1-2 micrograms of selenium/kg of body weight/day.
- 13. A method of producing L-Se-methylselenocysteine, comprising:
mixing N-(tert-butoxycarbonyl)-L-serine with a dialkyl diazodicarboxylate and at least one of a trialkylphosphine, triarylphosphine, and phosphite to form a first mixture that includes N-(tert-butoxycarbonyl)-L-serine β-lactone; mixing at least one of methyl selenol and a salt of methyl selenol with the N-(tert-butoxycarbonyl)-L-serine β-lactone to form a second mixture that includes N-(tert-butoxycarbonyl)-Se-methylselenocysteine, the N-(tert-butoxycarbonyl)-Se-methylselenocysteine having a tert-butoxycarbonyl group; and removing at least one tert-butoxycarbonyl group from the N-(tert-butoxycarbonyl)-Se-methylselenocysteine to form the L-Se-methylselenocysteine.
- 14. The method of claim 13 wherein said mixing to form a first mixture further includes mixing tetrahydrofuran with the first mixture under argon.
- 15. The method of claim 13 wherein the dialkyl diazodicarboxylate is diethyl azodicarboxylate, and the at least one of trialkylphosphine, triarylphosphine, and phosphite is triphenylphosphine.
- 16. The method of claim 15 wherein said removing comprises mixing trifluoroacetic acid with the second mixture.
- 17. The method of claim 13 wherein said mixing to form a first mixture occurs for about 15 minutes under a cooling bath at approximately −78° C.
- 18. The method of claim 17 further comprising allowing said first mixture to warm to room temperature following said mixing to form the first mixture.
- 19. The method of claim 18 further comprising concentrating the first mixture by rotary evaporation following said mixing to form the first mixture.
- 20. The method of claim 19 further comprising titrating the first mixture with 85:15 hexanes:ethyl acetate following said mixing to form the first mixture.
- 21. The method of claim 20 further comprising chromographing the first mixture using a gradient elution of 85:15 hexanes:ethyl acetate following said mixing to form the first mixture.
- 22. The method of claim 13 wherein sodium borohydride is mixed with the at least one of methyl selenol and salt of methyl selenol prior to obtaining the first mixture.
- 23. The method of claim 22 wherein the second mixture is mixed under argon at 0° C.
- 24. The method of claim 23 further comprising acidifying the second mixture with hydrochloric acid to a pH of about 2.0.
- 25. The method of claim 13 wherein said removing comprises mixing trifluoroacetic acid with the second mixture.
- 26. The method of claim 25 wherein said removing further comprises mixing the second mixture in dichloromethane under argon at room temperature.
- 27. A method of producing L-Se-methylselenocysteine, comprising:
mixing at least one of methyl selenol and a salt of methyl selenol with the L-serine β-lactone to form a mixture that includes L-Se-methylselenocysteine.
- 28. A method of claim 27 further comprising making L-serine β-lactone by mixing L-serine with a dialkyl diazodicarboxylate and at least one of a trialkylphosphine, triarylphosphine, and phosphite to form the L-serine β-lactone.
- 29. The method of claim 28 wherein the dialkyl diazodicarboxylate is diethyl azodicarboxylate, and the at least one of trialkylphosphine, triarylphosphine, and phosphite is triphenylphosphine.
CROSS REFERENCE TO RELATED APPLICATIONS
[0001] This application is a continuation-in-part of co-pending U.S. patent application Ser. No. 09/376,073, filed Aug. 16, 1999.
Divisions (1)
|
Number |
Date |
Country |
Parent |
09677563 |
Oct 2000 |
US |
Child |
10288024 |
Nov 2002 |
US |
Continuation in Parts (1)
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Number |
Date |
Country |
Parent |
09376073 |
Aug 1999 |
US |
Child |
09677563 |
Oct 2000 |
US |