Claims
- 1. A method for determining the genotype of a human papillomavirus present in a sample comprising the steps of:(a) amplifying a portion of the L1 open reading frame of human papillomavirus genome to form L1 amplicons containing plus and minus amplified strands using first and second amplification primers; and (b) determining the positions of at least one species of nucleotide within at least one of the plus and minus amplified strands by extension of a seuqenicng primer which hybridizes with the plus or minus amplified strand in the presence of a chain-terminating nucleotide, wherein the first amplification primer has the sequence given by Seq. ID No. 2, and the seuqenicng primer has the sequence given by Seq. ID No. 3.
- 2. The method according to claim 1, wherein the second amplification primer has the sequence given by Seq. ID No. 1.
- 3. The method according to claim 2, wherein only the positions of the A bases are determined in step (b).
- 4. The method according to claim 1, wherein only the positions of the A bases are determined in step (b).
- 5. The method according to claim 1, wherein the amplification is performed using an amplification primer having the sequence given by Seq. ID No. 4.
- 6. The method according to claim 5, wherein only the positions of the A bases are determined in step (b).
Parent Case Info
This application is a division of application Ser. No. 09/087,655 filed May 30, 1998, now U.S. Pat. No. 6,045,993, which is incorporated herein by reference.
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