Patent Application
20080145390
The present invention relates to a method of providing a rapid and a persistent control of viruses, and a rapid, broad-spectrum control of bacteria, on an animate or inanimate surface, and particularly on food contact surfaces. More particularly, the present invention relates to a method of controlling viruses and bacteria on surfaces by applying a compound or a composition to the surface that is capable of providing a surface pH of less than about 4, for a period of about four or more hours, without irritation or corrosion of the surface. The compound typically is (a) an organic acid, (b) an inorganic acid, (c) an inorganic salt, (d) an aluminum, zirconium, or aluminum-zirconium complex, or (e) mixtures thereof, capable of sufficiently lowering a surface pH to control viruses and bacteria. The surface optionally can be contacted with one or both of a disinfecting alcohol and an antimicrobial agent to assist in bacterial and viral control. In some embodiments, the compounds and compositions provide a barrier layer, or film, on a treated surface to impart a persistent antiviral activity to the surface. The method controls Gram positive and Gram negative bacterial populations, and viral populations, within one minute, and provides a persistent antiviral control for about four hours or more. The present invention also relates to articles comprising the compound or composition, and to methods of treating inanimate surfaces using the compound or composition.
Human health is impacted by a variety of microbes encountered on a daily basis. In particular, contact with various microbes in the environment can lead to an illness, possibly severe, in mammals. For example, microbial contamination can lead to a variety of illnesses, including, but not limited to, food poisoning, a streptococcal infection, anthrax (cutaneous), athlete's foot, cold sores, conjunctivitis (“pink eye”), coxsackievirus (hand-foot-mouth disease), croup, diphtheria (cutaneous), ebolic hemorrhagic fever, and impetigo.
It is known that washing body parts (e.g., hand washing) and hard surfaces (e.g., countertops and sinks) can significantly decrease the population of microorganisms, including pathogens. Therefore, cleaning skin, and other animate and inanimate surfaces, to reduce microbial populations is a first defense in removing such pathogens from these surfaces, and thereby minimizing the risk of infection.
Viruses are one category of pathogens that are of primary concern. Viral infections are among the greatest causes of human morbidity, with an estimated 60% or more of all episodes of human illness in developed countries resulting from a viral infection. In addition, viruses infect virtually every organism in nature, with high virus infection rates occurring among all mammals, including humans, pets, livestock, and zoo specimens.
Viruses exhibit an extensive diversity in structure and lifecycle. A detailed description of virus families, their structures, life cycles, and modes of viral infection is discussed in Fundamental Virology, 4th Ed., Eds. Knipe & Howley, Lippincott Williams & Wilkins, Philadelphia, Pa., 2001.
Simply stated, virus particles are intrinsic obligate parasites, and have evolved to transfer genetic material between cells and encode sufficient information to ensure their own propagation. In a most basic form, a virus consists of a small segment of nucleic acid encased in a simple protein shell. The broadest distinction between viruses is the enveloped and nonenveloped viruses, i.e., those that do or do not contain, respectively, a lipid-bilayer membrane.
Viruses propagate only within living cells. The principal obstacle encountered by a virus is gaining entry into the cell, which is protected by a cell membrane of thickness comparable to the size of the virus. In order to penetrate a cell, a virus first must become attached to the cell surface. Much of the specificity of a virus for a certain type of cell lies in its ability to attach to the surface of that specific cell. Durable contact is important for the virus to infect the host cell, and the ability of the virus and the cell surface to interact is a property of both the virus and the host cell. The fusion of viral and host-cell membranes allows the intact viral particle, or, in certain cases, only its infectious nucleic acid to enter the cell. Therefore, in order to control a viral infection, it is important to rapidly kill a virus that contacts the skin, and ideally to provide a persistent antiviral activity on the skin, or a hard surface, in order to control viral infections.
For example, rhinoviruses, influenza viruses, and adenoviruses are known to cause respiratory infections. Rhinoviruses are members of the picornavirus family, which is a family of “naked viruses” that lack an outer envelope. The human rhinoviruses are so termed because of their special adaptation to the nasopharyngeal region, and are the most important etiological agents of the common cold in adults and children. Officially, there are 102 rhinovirus serotypes. Most of the picornaviruses isolated from the human respiratory system are acid labile, and this lability has become a defining characteristic of rhinoviruses.
Rhinovirus infections are spread from person to person by direct contact with virus-contaminated respiratory secretions. Typically, this contact is in the form of physical contact with a contaminated surface, rather than via inhalation of airborne viral particles.
Rhinovirus can survive on environmental surfaces for hours after initial contamination. Rhinovirus infection is readily transmitted by finger-to-finger contact, and by contaminated environmental surface-to-finger contact, when the newly contaminated finger then rubs an eye or touches the nasal mucosa. Therefore, virus contamination of skin and environmental surfaces should be minimized to reduce the risk of transmitting the infection to the general population.
Several gastrointestinal infections also are caused by viruses. For example, Norwalk virus causes nausea, vomiting (sometimes accompanied by diarrhea), and stomach cramps. This infection typically is spread from person to person by direct contact. Acute hepatitis A viral infection similarly can be spread by direct contact between one infected person and a nonimmune individual by hand-to-hand, hand-to-mouth, or aerosol droplet transfer, or by indirect contact when an uninfected individual comes into contact with a hepatitis A virus-contaminated solid object. Numerous other viral infections are spread similarly. The risk of transmitting such viral infections can be reduced significantly by inactivating or removing viruses from the hands and other environmental surfaces.
Common household phenol/alcohol disinfectants are effective in disinfecting contaminated environmental surfaces, but lack persistent virucidal activity. Hand washing is highly effective in disinfecting contaminated fingers, but again suffers from a lack of persistent activity. These shortcomings illustrate the need for improved virucidal compositions having a persistent activity against viruses, such as rhinoviruses.
Antimicrobial personal care compositions are known in the art. In particular, antibacterial cleansing compositions, which typically are used to cleanse the skin and to destroy bacteria present on the skin, especially the hands, arms, and face of the user, are well-known commercial products.
Antibacterial compositions are used, for example, in the health care industry, food service industry, meat processing industry, and in the private sector by individual consumers. The widespread use of antibacterial compositions indicates the importance consumers place on controlling bacteria populations on skin. The paradigm for antibacterial compositions is to provide a substantial and broad spectrum reduction in bacterial populations quickly and without adverse side effects associated with toxicity and skin irritation. Such antibacterial compositions are disclosed in U.S. Pat. Nos. 6,107,261 and 6,136,771, each incorporated herein by reference.
One class of antibacterial personal care compositions is the hand sanitizers. This class of compositions is used primarily by medical personnel to disinfect the hands and fingers. A hand sanitizer is applied to, and rubbed into, the hands and fingers, and the composition is allowed to evaporate from the skin.
Hand sanitizers contain a high percentage of an alcohol, like ethanol. At the high percent of alcohol present in the gel, the alcohol itself acts as a disinfectant. In addition, the alcohol quickly evaporates to obviate wiping or rinsing skin treated with the sanitizer gel. Hand sanitizers containing a high percentage of an alcohol, i.e., about 40% or greater by weight of the composition, do not provide a persistent bacterial kill.
Antibacterial cleansing compositions typically contain an active antibacterial agent, a surfactant, and various other ingredients, for example, dyes, fragrances, pH adjusters, skin conditioners, and the like, in an aqueous and/or alcoholic carrier. Several different classes of antibacterial agents have been used in antibacterial cleansing compositions. Examples of antibacterial agents include bisguanidines (e.g., chlorhexidine gluconate), diphenyl compounds, benzyl alcohols, trihalocarbanilides, quaternary ammonium compounds, ethoxylated phenols, and phenolic compounds, such as halo-substituted phenolic compounds, like PCMX (i.e., p-chloro-m-xylenol) and triclosan (i.e., 2,4,4′-trichloro-2′-hydroxy-diphenylether). Antimicrobial compositions based on such antibacterial agents exhibit a wide range of antibacterial activity, ranging from low to high, depending on the microorganism to be controlled and the particular antibacterial composition.
Most commercial antibacterial compositions generally offer a low to moderate antibacterial activity, and no reported antiviral activity. Antibacterial activity is assessed against a broad spectrum of microorganisms, including both Gram positive and Gram negative microorganisms. The log reduction, or alternatively the percent reduction, in bacterial populations provided by the antibacterial composition correlates to antibacterial activity. A 1-3 log reduction is preferred, a log reduction of 3-5 is most preferred, whereas a log reduction of less than 1 is least preferred, for a particular contact time, generally ranging from 15 seconds to 5 minutes. Thus, a highly preferred antibacterial composition exhibits a 3-5 log reduction against a broad spectrum of microorganisms in a short contact time.
Virus control poses a more difficult problem, however. By sufficiently reducing bacterial populations, the risk of bacterial infection is reduced to acceptable levels. Therefore, a rapid antibacterial kill is desired. With respect to viruses, however, not only is a rapid kill desired, but a persistent antiviral activity also is required. This difference is because merely reducing a viral population is insufficient to reduce infection. In theory, a single virus can cause infection. Therefore, an essentially total, and persistent, antiviral activity is required, or at least desired, for an effective antiviral cleansing composition.
WO 98/01110 discloses compositions comprising triclosan, surfactants, solvents, chelating agents, thickeners, buffering agents, and water. WO 98/01110 is directed to reducing skin irritation by employing a reduced amount of surfactant.
U.S. Pat. No. 5,635,462 discloses compositions comprising PCMX and selected surfactants. The compositions disclosed therein are devoid of anionic surfactants and nonionic surfactants.
EP 0 505 935 discloses compositions containing PCMX in combination with nonionic and anionic surfactants, particularly nonionic block copolymer surfactants.
WO 95/32705 discloses a mild surfactant combination that can be combined with antibacterial compounds, like triclosan.
WO 95/09605 discloses antibacterial compositions containing anionic surfactants and alkylpolyglycoside surfactants.
WO 98/55096 discloses antimicrobial wipes having a porous sheet impregnated with an antibacterial composition containing an active antimicrobial agent, an anionic surfactant, an acid, and water, wherein the composition has a pH of about 3.0 to about 6.0.
U.S. Pat. No. 6,110,908 discloses a topical antiseptic containing a C2-3 alcohol, a free fatty acid, and zinc pyrithione.
N. A. Allawala et al., J. Amer. Pharm. Assoc.—Sci. Ed., Vol. XLII, no. 5, pp. 267-275 (1953) discusses the antibacterial activity of active antibacterial agents in combination with surfactants.
A. G. Mitchell, J. Pharm. Pharmacol., Vol. 16, pp. 533-537 (1964) discloses compositions containing PCMX and a nonionic surfactant that exhibit antibacterial activity.
With respect to hand sanitizer gels, U.S. Pat. No. 5,776,430 discloses a topical antimicrobial cleaner containing chlorhexidine and an alcohol. The compositions contain about 50% to 60%, by weight, denatured alcohol and about 0.65% to 0.85%, by weight, chlorhexidine. The composition is applied to the skin, scrubbed into the skin, then rinsed from the skin.
European Patent Application 0 604 848 discloses a gel-type hand disinfectant containing an antimicrobial agent, 40% to 90% by weight of an alcohol, and a polymer and a thickening agent in a combined weight of not more than 3% by weight. The gel is rubbed into the hands and allowed to evaporate to provide disinfected hands. The disclosed compositions often do not provide immediate sanitization and do not provide persistent antimicrobial efficacy. As illustrated in EP 0 604 848, the amount and identity of the antibacterial agent is not considered important because the hand sanitizer gels contain a high percentage of an alcohol to provide antibacterial activity.
In general, hand sanitizer gels typically contain: (a) at least 60% by weight ethanol or a combination of lower alcohols, such as ethanol and isopropanol, (b) water, (c) a gelling polymer, such as a crosslinked polyacrylate material, and (d) other ingredients, such as skin conditioners, fragrances, and the like. Hand sanitizer gels are used by consumers to effectively sanitize the hands, without, or after, washing with soap and water, by rubbing the hand sanitizer gel on the surface of the hands. Current commercial hand sanitizer gels rely on high levels of alcohol for disinfection and evaporation, and thus suffer from disadvantages. Specifically, because of the volatility of ethanol, the primary active disinfectant does not remain on the skin after use, thus failing to provide a persistent antimicrobial effect.
At alcohol concentrations below 60%, ethanol is not recognized as an antiseptic. Thus, in compositions containing less than 60% alcohol, an additional antimicrobial compound typically is present to provide antimicrobial activity. Prior disclosures, however, have not addressed the issue of which composition ingredient in such an antimicrobial composition provides microbe control. Therefore, for formulations containing a reduced alcohol concentration, the selection of an antimicrobial agent that provides both a rapid antimicrobial effect and a persistent antimicrobial benefit is difficult.
U.S. Pat. Nos. 6,107,261 and 6,136,771 disclose highly effective antibacterial compositions. These patents disclose compositions that solve the problem of controlling bacteria on skin and hard surfaces, but are silent with respect to controlling viruses.
U.S. Pat. Nos. 5,968,539; 6,106,851; and 6,113,933 disclose antibacterial compositions having a pH of about 3 to about 6. The compositions contain an antibacterial agent, an anionic surfactant, and a proton donor.
A composition containing a quaternary ammonium compound and a selected anionic surfactant has been disclosed as being effective in some applications (e.g., U.S. Pat. No. 5,798,329), but no reference disclosing such a combination for use in personal care compositions has been found.
Patents and published applications disclosing germicidal compositions containing a quaternary ammonium antibacterial agent include U.S. Pat. Nos. 5,798,329 and 5,929,016; WO 97/15647; and EP 0 651 048, directed to antibacterial laundry detergents and antibacterial hard surface cleaners.
Antiviral compositions that inactivate or destroy pathogenic viruses, including rhinovirus, rotavirus, influenza virus, parainfluenza virus, respiratory syncytial virus, and Norwalk virus, also are known. For example, U.S. Pat. No. 4,767,788 discloses the use of glutaric acid to inactivate or destroy viruses, including rhinovirus. U.S. Pat. No. 4,975,217 discloses compositions containing an organic acid and an anionic surfactant, for formulation as a soap or lotion, to control viruses. U.S. Patent Publication 2002/0098159 discloses the use of a proton donating agent and a surfactant, including an antibacterial surfactant, to effect antiviral and antibacterial properties.
U.S. Pat. No. 6,034,133 discloses a virucidal hand lotion containing malic acid, citric acid, and a C1-6 alcohol. U.S. Pat. No. 6,294,186 discloses combinations of a benzoic acid analog, such as salicyclic acid, and selected metal salts as being effective against viruses, including rhinovirus. U.S. Pat. No. 6,436,885 discloses a combination of known antibacterial agents with 2-pyrrolidone-5-carboxylic acid, at a pH of 2 to 5.5, to provide antibacterial and antiviral properties.
Organic acids in personal washing compositions also have been disclosed. For example, WO 97/46218 and WO 96/06152 disclose the use of organic acids or salts, hydrotropes, triclosan, and hydric solvents in a surfactant base for antimicrobial cleansing compositions. These publications are silent with respect to antiviral properties.
Hayden et al., Antimicrobial Agents and Chemotherapy, 26:928-929 (1984), discloses interrupting the hand-to-hand transmission of rhinovirus colds through the use of a hand lotion having residual virucidal activity. The hand lotions, containing 2% glutaric acid, were more effective than a placebo in inactivating certain types of rhinovirus. However, the publication discloses that the glutaric acid-containing lotions were not effective against a wide spectrum of rhinovirus serotypes.
A virucidal tissue designed for use by persons infected with the common cold, and including citric acid, malic acid, and sodium lauryl sulfate, is known. Hayden et al., Journal of Infectious Diseases, 152:493-497 (1985), however, reported that use of paper tissues, either treated with virus-killing substances or untreated, can interrupt the hand-to-hand transmission of viruses. Hence, no distinct advantage in preventing the spread of rhinovirus colds can be attributed to the compositions incorporated into the virucidal tissues.
U.S. Pat. No. 4,503,070 discloses a method of treating a common cold by the topical application of zinc gluconate to the oral mucosa. The method reduces the duration of the cold by alleviating common cold symptoms. U.S. Pat. No. 5,409,905 also discloses a method of treating a common cold by applying a solid composition containing zinc ions to the oral and oropharyngeal membranes of a human. U.S. Pat. No. 5,622,724 discloses a treatment for the common cold comprising administering a spray comprising a solution of a substantially unchelated ionic zinc compound to the nostrils and respiratory tract of a patient in need. U.S. Pat. No. 6,673,835 discloses a method and composition for delivering a low, but effective, amount of a zinc-containing active ingredient into the blood via application to the nasal cavity.
An efficacious method of controlling both bacterial and viral populations has been difficult to achieve because of the fundamental differences between a bacteria and a virus. Even more difficult to achieve is a method that provides a persistent antiviral activity. Although a number of antimicrobial products currently exist, taking a variety of product forms (e.g., deodorant soaps, hard surface cleaners, and surgical disinfectants), such antimicrobial products typically incorporate high levels of an alcohol and/or surfactants, which can dry out and irritate skin tissues. Ideally, personal antimicrobial compositions and methods gently cleanse the skin, cause little or no irritation, and do not leave the skin overly dry after frequent use.
Accordingly, a need exists for a method that is highly efficacious in controlling a broad spectrum of microbes, including viruses and Gram positive and Gram negative bacteria, on surfaces, and especially on food contact surfaces, in a short time period, and wherein the method provides a persistent antiviral activity, and is mild to the surface. Methods providing an improved reduction in virus and bacteria populations are achieved by the present invention, including methods of providing a persistent reduction in virus populations.
The present invention is directed to methods and articles that provide a rapid antiviral and antibacterial control, and a persistent antiviral control, on surfaces, and particularly on hard surfaces found where food is processed, prepared, stored, and sold. The method provides a substantial viral control and a substantial reduction in Gram positive and Gram negative bacteria in less than about one minute.
More particularly, the present invention provides a method of killing a broad spectrum of bacteria, including Gram positive and Gram negative bacteria such as S. aureus, S. choleraesuis, E. coli, and K. pneumoniae, while simultaneously inactivating or destroying viruses harmful to human health, particularly acid-labile viruses, and especially rhinoviruses and other acid-labile picornaviruses. Influenza viruses and noroviruses also are controlled.
Accordingly, one aspect of the present invention is to provide a method of controlling viruses and bacteria on mammalian skin comprising contacting a hard or soft inanimate surface with a compound or composition capable of lowering surface pH to less than about 4, without irritating the surface. In some embodiments, the method provides a broad spectrum bacterial control and a persistent viral control for up to about eight hours. The composition has a pH of about 5 or less and provides an essentially continuous layer or film of the composition ingredients on a treated surface to impart a persistent antiviral activity to the treated surface. In preferred embodiments, the compositions further comprise a gelling agent. An optional active antibacterial agent also can be included in the composition.
Another aspect of the present invention is to provide a method of controlling bacteria and viruses on a surface comprising applying a composition containing an organic acid, an inorganic acid, an inorganic salt, an aluminum, zirconium, or aluminum-zirconium complex, or mixtures thereof, to the skin to sufficiently lower surface pH and thereby control bacteria and viruses, without irritating the skin.
Still another aspect of the present invention is to provide a method of controlling bacteria and viruses on an animate or inanimate surface, for an extended time, comprising contacting the surface with an aqueous antimicrobial composition containing a compound selected from the group consisting of (a) an organic acid selected from the group consisting of a monocarboxylic acid, a polycarboxylic acid, a polymeric acid having a plurality of carboxylic, phosphate, sulfonate, and/or sulfate moieties, and mixtures thereof; (b) an inorganic acid that is nonirritating to the skin; (c) an inorganic salt comprising a cation having a valence of 2, 3, or 4 and a counterion, (d) an aluminum, zirconium, or aluminum-zirconium complex, and (e) mixtures thereof, wherein the composition is capable of reducing surface pH to less than about 4. The composition has a pH of about 5 or less, and is capable of providing a residual layer of composition components on a treated surface.
Another aspect of the present invention is to provide an antimicrobial composition having antibacterial and antiviral activity that is substantiative to the surface, and/or that fails to penetrate the surface, and/or that resists rinsing from the surface, and/or that forms an essentially continuous barrier layer on the surface, for example, hydrophobic monocarboxylic acids, polycarboxylic acids, polymeric acids having a plurality of carboxylic, phosphate, sulfonate, and/or sulfate moieties, or mixtures thereof, and (c) water, wherein the composition has a pH of about 5 or less. Such organic acids typically have a log P of less than one, and the compositions are effective against a broad spectrum of bacteria and exhibit a synergistic activity against nonenveloped viruses. The compositions also are effective against influenza viruses and noroviruses. The persistent antiviral activity is attributed, in part, to a residual layer or film comprising the organic acid on a treated surface, which resists removal from the surface after several rinsings, and during normal daily routines for a period of several hours. Preferred compositions comprise one or more polycarboxylic acid, a polymeric acid, and a gelling agent. These compositions provide an effective and persistent control of nonenveloped viruses and exhibit a synergistic activity against Gram positive and Gram negative bacteria.
In preferred embodiments, the composition provides an essentially continuous layer or film of the nonvolatile composition ingredients on a treated surface to impart a persistent antiviral activity to the treated surface. In other preferred embodiments, the composition is free of an intentionally-added surfactant.
Preferred compositions comprise one or more polycarboxylic acid, a polymeric acid, and a gelling agent. These compositions provide an effective and persistent control of viruses and exhibit a synergistic activity against Gram positive and Gram negative bacteria.
Another aspect of the present invention is to provide product forms for delivery of the antimicrobial composition, including solid, semisolid, gel, and liquid product forms.
Another aspect of the present invention is to provide a method that achieves a substantial, wide spectrum bacterial control, and a persistent viral control, on a treated surface.
Yet another aspect of the present invention is to provide a method that achieves a log reduction against Gram positive bacteria (i.e., S. aureus) of at least 2 after 30 seconds of contact.
Still another aspect of the present invention is to provide a method that achieves a log reduction against Gram negative bacteria (i.e., E. coli) of at least 2.5 after 30 seconds of contact.
Another aspect of the present invention is to provide a method that achieves a log reduction against acid-labile viruses, including rhinovirus serotypes, such as Rhinovirus 1a, Rhinovirus 14, Rhinovirus 2, and Rhinovirus 4, of at least 4 on mammalian skin after 30 seconds of contact. The antimicrobial composition also provides a log reduction against nonenveloped viruses of at least 3 for at least about five hours, and at least 2 for about six hours, after application with a 30 second contact time. In some embodiments, the antimicrobial composition provides a log reduction against nonenveloped viruses of 2 for up to about eight hours.
Another aspect of the present invention is to provide a method that achieves a persistent antiviral activity, e.g., about four hours or more, after application of a compound or composition to the surface. The present method achieves a persistent antiviral activity on inanimate surfaces, e.g., food contact surfaces, after application of the compound or composition to the inanimate surface.
Another aspect of the present invention is to provide an antimicrobial composition that resists rinsing from the surface, e.g., at least 50%, at least 60%, and preferably at least 70% of the nonvolatile components of an applied composition remains on a treated surface after three water rinsings and an effective antiviral amount of the composition remains on the skin after ten water rinsings.
Yet another aspect of the present invention is to provide consumer products, for example, a skin cleanser, a body splash, a surgical scrub, a wound care agent, a hand sanitizer, a disinfectant, a pet shampoo, a hard or soft surface sanitizer, a lotion, an ointment, a paste, a solid, a cream, and the like, capable of reducing the pH of a surface, like mammalian skin, to less than about 4 to effect a rapid, broad spectrum, bacterial control and a persistent viral control, without irritating the skin. The consumer product can be a rinse-off product or a leave-on product. Preferably, the product is allowed to remain on the treated surfaces to allow the pH lowering components of the product to remain on, and preferably substantively deposit on, the surfaces to enhance a persistent antiviral control. The compositions are esthetically pleasing and nonirritating to the surface, and provide an essentially continuous residual film or layer of the nonvolatile composition components, e.g., the organic acid, on the surface.
A further aspect of the present invention is to provide a method of quickly controlling a wide spectrum of viruses and the Gram positive and/or Gram negative bacteria populations on animal tissue, including human tissue, by contacting the tissue, like the dermis, with a compound or composition for a sufficient time, for example, about 15 seconds to 5 minutes or longer, e.g., about one hour, to reduce tissue pH to less than about 4 and thereby reduce bacterial and viral populations to a desired level. A further aspect of the present invention is to provide a method that achieves a persistent control of viruses on animal tissue.
Still another aspect of the present invention is to provide a method treating or preventing virus-mediated diseases and conditions caused by rhinoviruses, rotaviruses, picornaviruses, adenoviruses, herpes viruses, respiratory syncytial viruses (RSV), coronaviruses, enteroviruses, and other nonenveloped viruses. The method also treats and prevents influenza-mediated and norovirus-mediated diseases and conditions.
Yet another aspect of the present invention is to provide a method of interrupting transmission of a virus from animate and inanimate surfaces to an animate surface, especially mammalian skin. Especially provided is a method of controlling the transmission of nonenveloped viruses, particularly, rhinoviruses by effectively controlling viruses present on human skin and inanimate surfaces, and continuing to control the viruses for a period of about four hours or more, and up to about eight hours, after application of a suitable compound or composition to the skin.
These and other novel aspects and advantages of the present invention are set forth in the following, nonlimiting detailed description of the preferred embodiments.
a and 1b are reflectance micrographs showing a barrier layer of nonvolatile components on a surface provided by application of a composition of the present invention to the surface, and
c and 1d are reflectance micrographs showing the absence of a barrier layer on a surface after application of a control composition to the surface.
Personal care products incorporating an active antimicrobial agent have been known for many years. Since the introduction of antimicrobial personal care products, many claims have been made that such products provide antimicrobial properties. To be most effective, an antimicrobial composition should provide a high log reduction against a broad spectrum of organisms in as short a contact time as possible. Ideally, the composition also should inactivate viruses.
As presently formulated, most commercial liquid antibacterial soap compositions provide a poor to marginal time kill efficacy, i.e., rate of killing bacteria. These compositions do not effectively control viruses.
Antimicrobial hand sanitizer compositions typically do not contain a surfactant and rely upon a high concentration of an alcohol to control bacteria. The alcohols evaporate and, therefore, cannot provide a persistent microbial control. The alcohols also can dry and irritate the skin.
Most current products especially lack efficacy against Gram negative bacteria, such as E. coli, which are of particular concern to human health. Compositions do exist, however, that have an exceptionally high broad spectrum antibacterial efficacy, as measured by a rapid kill of bacteria (i.e., time kill), which is to be distinguished from persistent kill. These products also lack a sufficient antiviral activity.
The present method is directed to providing an excellent broad spectrum antibacterial efficacy and a significantly improved antiviral efficacy compared to prior methods and compositions that utilize a high percentage of an alcohol, i.e., 40% or greater, by weight. The basis of this improved efficacy is the discovery that reducing the pH of a surface, such as mammalian skin, including human skin, provides a rapid, broad spectrum control of bacteria and a rapid and persistent control of viruses. An important aspect of the present invention is to maintain a low surface pH for an extended time to provide a persistent antiviral activity. In preferred embodiments, this is achieved by forming an essentially continuous film of nonvolatile composition components on the surface, which provides a reservoir of the compounds that maintain a low skin pH.
The term “essentially continuous film” means that a residue of the nonvolatile components of the composition in the form of a barrier layer is present on at least 50%, at least 60%, at least 70%, or at least 80%, preferably at least 85% or at least 90%, and more preferably at least 95%, of the area of the treated surface area. An “essentially continuous” film is demonstrated in the reflectance micrographs of the figures, which are discussed hereafter. The term “essentially continuous film” as used herein is synonymous with the term “essentially continuous layer”, “barrier layer”, and “barrier film”.
Although compositions containing an antimicrobial agent, like triclosan, have demonstrated a rapid and effective antibacterial activity against Gram positive and Gram negative bacteria, control of viruses has been inadequate. Virus control on skin and inanimate surfaces is very important in controlling the transmission of numerous diseases.
For example, rhinoviruses are the most significant microorganisms associated with the acute respiratory illness referred to as the “common cold.” Other viruses, such as parainfluenza viruses, respiratory syncytial viruses (RSV), enteroviruses, and coronaviruses, also are known to cause symptoms of the “common cold,” but rhinoviruses are theorized to cause the greatest number of common colds. Rhinoviruses also are among the most difficult of the cold-causing viruses to control, and have an ability to survive on a hard dry surface for more than four days. In addition, most viruses are inactivated upon exposure to a 70% ethanol solution. However, rhinoviruses remain viable upon exposure to ethanol.
Because rhinoviruses are the major known cause of the common cold, it is important that a composition having antiviral activity controls rhinovirus serotypes. Although the molecular biology of rhinoviruses is now understood, finding effective methods for preventing colds caused by rhinoviruses, and for preventing the spread of the virus to noninfected subjects, has been fruitless.
It is known that iodine is an effective antiviral agent, and provides persistent antirhinoviral activity on skin. In experimentally induced and natural cold transmission studies, subjects who used iodine products had significantly fewer colds than placebo users. This indicates that iodine is effective for prolonged periods at blocking the transmission of rhinoviral infections. Thus, the development of products that deliver both immediate and persistent antiviral activity would be effective in reducing the incidence of colds. Likewise, a topically applied composition that exhibits antiviral activity would be effective in preventing and/or treating diseases caused by other acid-labile viruses.
A rotavirus also is a virus that is stable in the environment. Rotavirus infection is an infection of the digestive tract, and is the most common cause of severe diarrhea among children, resulting in over 50,000 hospitalizations yearly in the U.S. alone. Rotaviral infections are particularly problematic in close communities, such as child care facilities, geriatric facilities, family homes, and children's hospitals.
The most common mode of transmitting rotavirus is person to person spread through contaminated hands, but transmission also can occur through ingestion of contaminated water or food, or through contact with contaminated surfaces. The rotavirus then enters the body through contact with the mouth.
It is known that washing hands and inanimate surfaces with soap and/or other cleansers does not kill rotavirus, but helps prevent its spread. An oral rotavirus vaccine has been approved for use in children in the U.S., but its use is not recommended because of a severe adverse side effect. Because no other effective way to eliminate rotavirus, or its spread, is currently available, workers in close communities, especially those catering to children, must adhere to strict hygienic practices to help curtail the spread of rotavirus. An improved composition having enhanced antiviral efficacy, including persistent antiviral efficacy, in inactivating rotaviruses would further curtail the spread of rotavirus infections.
Virucidal means capable of inactivating or destroying a virus. As used herein, the term “persistent antiviral efficacy” or “persistent antiviral activity” means leaving a residue or imparting a condition on animate (e.g., skin) or inanimate surfaces that provides significant antiviral activity for an extended time after application. In some embodiments, a “persistent antiviral efficacy” or “persistent antiviral activity” means leaving a barrier residue, layer, or film of antiviral agents, including organic acids, on animate (e.g., skin) or inanimate surfaces that provides significant antiviral activity for an extended time after application. The barrier residue layer or film can be continuous or essentially continuous, and resists removal from a treated surface during water rinsing.
A method of the present invention provides a persistent antiviral efficacy, i.e., preferably a log reduction of at least 3, and more preferably a log reduction of at least log 4, against pathogenic acid-labile viruses, such as rhinovirus serotypes, within 30 seconds. Antiviral activity is maintained for at least about 0.5 hour, preferably at least about 1 hour, and more preferably at least about two hours, at least about three hours, or at least about four hours after contact with a suitable compound or composition. In some preferred embodiments, antiviral activity is maintained for about six to about eight hours after contact with the compound or composition. The persistent antiviral activity is attributed, at least in part, to the reservoir of nonvolatile components present in the barrier layer or film of the composition on a treated surface. The methodology utilized to determine a persistent antiviral efficacy is discussed below.
The method of the present invention, therefore, is highly effective in providing a rapid and broad spectrum control of bacteria, and a rapid and persistent control of viruses. It has been discovered that persistent antiviral benefits can be imparted to mammalian skin by reducing the skin pH to less than about 4, preferably less than about 3.75, and more preferably less than about 3.5, and most preferably less than about 3.25 by any safe and effective means, typically by contacting the skin with a suitable compound or composition.
Compounds and compositions effective at inactivating or otherwise destroying bacteria and viruses are known, but these compositions and methods rely on the pH of the composition and/or the active ingredients of the compositions to effect viral and bacterial control. Surprisingly, it has been discovered that a rapid and broad spectrum bacterial control, and a persistent viral control, can be achieved by reducing a surface pH to less than about 4. Thus, the present method provides a safer, milder, and more efficacious approach to the problem of viral and bacterial control than prior methods and compositions.
The method not only is mild to the skin, but also noncorrosive to inanimate surfaces. Thus, an effective method that solves the problem of bacterial and viral control an inanimate surface also is provided.
The present compositions provide an effective and persistent inactivation of nonenveloped viruses. Nonenveloped viruses include, but are not limited to, adenoviruses, papovaviruses, parvoviruses, birnaviruses, astroviruses, rotaviruses, caliciviruses (including Norwalk virus), and picornaviruses (including rhinovirus, polio virus, and hepatitis A virus). The compositions also effectively control and inactivate influenza viruses and noroviruses.
The present method comprises contacting a surface, and particularly mammalian skin or a food contact surface, with a compound or a composition that lowers the pH of the surface to less than about 4, such as down to about 2.5. Thus, present method is highly efficacious in personal care applications (e.g., lotions, shower gels, soaps, shampoos, and wipes), industrial and healthcare applications (e.g., sterilization of instruments, medical devices, and gloves), household cleaning applications (e.g., hard surfaces, like floors, countertops, tubs, dishes, and soft cloth materials, like clothing and bedding), industrial, cruise ship, nursing home, school, medical office, dental office, and hospital applications (e.g., sterilization of instruments, medical devices, linens, dressing gowns, and gloves). The present method efficaciously and rapidly disinfects surfaces that are infected or contaminated with Gram negative bacteria, Gram positive bacteria, and nonenveloped viruses (e.g., rhinoviruses). The present method also provides a persistent antiviral effectiveness.
The present method can be used in vitro and in vivo. In vitro means in or on nonliving things, especially on inanimate objects having hard or soft surfaces located or used where preventing viral transmission is desired, most especially on objects that are touched by human hands. In vivo means in or on animate objects, especially on mammal skin, and particularly on hands.
The present method comprises contacting a surface with a compound or a composition that reduces skin pH to less than about 4, and preferably less than about 3.75, less than about 3.5, less than abut 3.25, less than about 3.0, and down to a pH of about 2.5, and that maintains a low skin pH over a period of up to about four hours, and in some embodiments up to about eight hours. The compound is applied to the surface in an amount of at least 10 micrograms of the compound per square centimeter of the surface. The method is highly effective in controlling a broad spectrum of bacteria, including Gram positive and Gram negative bacteria, such as S. aureus, S. choleraesuis, E. coli, and K. pneumoniae, as well as simultaneously inactivating or otherwise destroying viruses harmful to human health, especially rhinovirus, for extended periods of time of about four hours or longer. The present method also is effective in controlling bacteria and viruses on inanimate surfaces.
In particular, the present method comprises contacting a surface in a transient fashion, such as washing and rinsing, or contacting a surface over a longer period, such as by applying a lotion, cream, gel, powder, or other solid or semisolid without rinsing, with a compound or composition capable of reducing the pH of the surface to less than about 4, and more preferably below about 3.75, for a period of time of up to about five hours, in preferred embodiments up to about eight hours, and at least about one-half hour.
As discussed more fully hereafter, compounds capable of lowering a surface pH include, but are not limited to, (a) an organic acid, preferably an acid that is substantive to the surface and having a pKa of about 1 to about 6, more preferably about 2 to about 5.5, most preferably about 2.5 to about 5, wherein pKa is the negative base ten logarithm of the acid dissociation constant of the acid in water at room temperature (25° C.), including organic polymeric acids, preferably capable of forming a substantive film on a skin surface and having a glass transition temperature, Tg, of less than about 25° C., preferably less than about 20° C., and more preferably less than about 15° C.; (b) an inorganic acid that is noncorrosive to the skin and other surfaces; (c) an inorganic salt solution, such as a solution of a salt MX, wherein M is a multivalent cation and X is an anion such that MX has a solubility in water of at least 0.1 g/100 ml at 25° C. and the pH of the solution is less than about 6, preferably less than about 5, more preferably less than about 4.5; (d) an aluminum, zirconium, or aluminum-zirconium complex; and (e) mixtures thereof.
The above and other compounds capable of lowering skin pH can be incorporated into consumer-acceptable compositions for an effective and esthetic application to the skin. Such compositions can contain other ingredients, such as additional antimicrobial agents, like a triclosan, a trichlorocarbanilide, a peroxide, a quaternary ammonium antimicrobial agent, a pyrithione salt, and a cosmetic preservative, and similar compounds, in an amount of 0% to about 5%, by weight of the composition. In preferred embodiments, the composition contains an optional gelling agent.
The compositions have a pH of less than about 5, and are capable of forming an essentially continuous film or layer of nonvolatile composition ingredients on a treated surface. The film or layer resists removal from the treated surface for several hours after application. In particular, an effective amount of composition ingredients remain on a treated surface after ten water rinsings, and at least 50%, preferably at least 60%, and more preferably at least 70%, of the nonvolatile composition ingredients remains on a treated surface after three water rinsings.
In embodiments where skin is treated, “rinsing” means gently rubbing treated skin for about 30 seconds under a moderate flow of tap water having a temperature of about 30° C. to about 40° C., then air drying the skin. In embodiments where an inanimate surface is treated, “rinsing” means contacting the treated surface for about 30 seconds under a moderate flow of tap water having a temperature of about 30° C. to about 40° C., then air drying the surface.
The present method exhibits a log reduction against Gram positive bacteria of about 2 after 30 seconds contact. The method also exhibits a log reduction against Gram negative bacteria of about 2.5 after 30 seconds contact. In addition to a rapid control of Gram positive and Gram negative bacteria, the present method also provides a persistent viral control.
The method further exhibits a log reduction against acid-labile viruses, including rhinovirus serotypes of about 4 after 30 seconds contact, and a log reduction against these acid-labile viruses of at least 3 about five hours after contact, and at least about 2 about six to about eight hours after skin contact with a suitable compound or composition. The method also is mild, and it is not necessary to rinse or wipe the compound or composition from the surface.
In accordance with the invention, a present antimicrobial composition can further comprise additional optional ingredients disclosed hereafter, like hydrotropes, polyhydric solvents, gelling agents, surfactants, pH adjusters, vitamins, dyes, skin conditioners, perfumes, and active antimicrobial agents, such as phenolic and quaternary ammonium antimicrobial agents. The compositions preferably are free of intentionally added cleansing surfactants, like anionic surfactants.
The following compounds are capable of sufficiently lowering skin pH in accordance with the method of the present invention.
A present method can utilize an organic acid in a sufficient amount to reduce a surface pH to less than about 4, and thereby control and inactivate bacteria and viruses on a surface contacted by the organic acid. The organic acid helps provide a rapid control of acid-labile viruses, and provides a persistent viral control.
Upon application to a surface, such as human skin, the pH of the surface is sufficiently lowered such that a persistent viral control is achieved. In preferred embodiments, a residual amount of the organic acid remains on the surface, even after a rinsing step, in order to impart a persistent viral control. However, after three rinsings, at least 50% of nonvolatile composition ingredients remain on the surface, and an effective amount of the composition remains on the treated surface after ten rinsings. Even if the organic acid is essentially completely rinsed from the surface, the surface pH has been sufficiently lowered to impart a viral control for at least 0.5 hours.
In particular, an organic acid is applied to a surface in a sufficient amount such that the pH of the animate or inanimate surface contacted by the organic acid is lowered to degree wherein a persistent viral control is achieved, i.e., to less than about 4. This persistent viral control is achieved regardless of whether the organic acid is rinsed from, or allowed to remain on, the contacted surface. The organic acid remains at least partially undissociated after application, and remains so when diluted, or during application and rinsing.
The organic acid has a pKa of about 1 to about 6, and preferably about 2 to about 5.5. To achieve the full advantage of the present invention, the organic acid has a pKa of about 2.5 to about 5. Such organic acids have a sufficient acid strength to reduce a surface pH to less than about 4. Preferably, the organic acid is substantive to the treated surface to enhance the persistent antimicrobial properties.
Typically, an organic acid is included in a composition in an amount of about 0.05% to about 15%, and preferably about 0.1% to about 10%, by weight of the composition. To achieve the full advantage, the organic acid is present in a composition in an amount of about 0.15% to about 6%, by weight of the composition. In preferred embodiments, a mixture of organic acids is included in the composition. The total amount of organic acid is related to the class of organic acid used, and to the identity of the specific acid or acids used.
An organic acid included in a present antimicrobial composition preferably does not penetrate the surface to which it is applied, e.g., remains on the surface as opposed to penetrating the surface and forms a layer or film on the surface, together with other nonvolatile composition ingredients, e.g., an optional gelling agent and/or active antibacterial agent. The organic acid, therefore, preferably is a hydrophobic organic acid.
In one embodiment of the present invention, the organic acid has a log P of less than one, and preferably less than 0.75. To achieve the full advantage of the present invention, the organic acid has a log P of less than 0.5. In this embodiment, an optional disinfecting alcohol and an organic acid act synergistically to provide an effective and persistent viral control.
In another embodiment, the organic acid has a log P of 1 or greater, for example, 1 to about 100. In this embodiment, an optional disinfecting alcohol and an organic acid effectively control nonenveloped viruses and also act synergistically to control a broad spectrum of bacteria.
It is envisioned that, by incorporating a first organic acid having a log P of less than one and a second organic acid having a log P of 1 or greater into a present composition, the first and second organic acids act synergistically with the optional disinfecting alcohol to provide a persistent control of nonenveloped viruses and a broad spectrum bacteria control.
As used herein, the term “log P” is defined as the log of the water-octanol partition coefficient, i.e., the log of the ratio Pw/Po, wherein Pw is the concentration of an organic acid in water and Po is the concentration of the organic acid in octanol, at equilibrium and 25° C. The water-octanol coefficient is determined by the U.S. Environmental Protection Agency Procedure, “OPPTS 830.7560 Partition Coefficient (n-Octanol/Water), Generator Column Method” (1996).
Organic acids having a log P less than one typically are water insoluble, e.g., have a water solubility of less than about 0.5 wt % at 25° C. Organic acids having a log P of one or greater typically are considered water soluble, e.g., have a water solubility of at least 0.5 wt %, at 25° C.
An organic acid useful in a present method comprises a monocarboxylic acid, a polycarboxylic acid, a polymeric acid having a plurality of carboxylic, phosphate, sulfonate, and/or sulfate moieties, or mixtures thereof. In addition to acid moieties, the organic acid also can contain other moieties, for example, hydroxy groups and/or amino groups. In addition, an organic acid anhydride can be used in the present method as the organic acid. Preferred organic acids are polycarboxylic acids, polymeric carboxylic acids, or a mixture thereof.
In one embodiment, the organic acid comprises a monocarboxylic acid having a structure RCO2H, wherein R is C1-10alkyl, hydroxyC1-3alkyl, haloC1-3alkyl, phenyl, or substituted phenyl. The monocarboxylic acid preferably has a water solubility of at least about 0.05%, by weight, at 25° C. The alkyl groups can be substituted with phenyl groups and/or phenoxy groups, and these phenyl and phenoxy groups can be substituted or unsubstituted.
Nonlimiting examples of monocarboxylic acids useful in the present invention are acetic acid, propionic acid, octanoic acid, hydroxyacetic acid, lactic acid, benzoic acid, phenylacetic acid, phenoxyacetic acid, zimanic acid, 2-, 3-, or 4-hydroxybenzoic acid, anilic acid, o-, m-, or p-chlorophenylacetic acid, o-, m-, or p-chlorophenoxyacetic acid, and mixtures thereof. Additional substituted benzoic acids are disclosed in U.S. Pat. No. 6,294,186, incorporated herein by reference. Examples of substituted benzoic acids include, but are not limited to, salicyclic acid, 2-nitrobenzoic acid, thiosalicylic acid, 2,6-dihydroxybenzoic acid, 5-nitrosalicyclic acid, 5-bromosalicyclic acid, 5-iodosalicyclic acid, 5-fluorosalicylic acid, 3-chlorosalicylic acid, 4-chlorosalicyclic acid, and 5-chlorosalicyclic acid.
In another embodiment, the organic acid comprises a polycarboxylic acid. The polycarboxylic acid contains at least two, and up to four, carboxylic acid groups. The polycarboxylic acid also can contain hydroxy or amino groups, in addition to substituted and unsubstituted phenyl groups. Preferably, the polycarboxylic acid has a water solubility of at least about 0.05%, by weight, at 25° C.
Nonlimiting examples of polycarboxylic acids useful in the present invention include malonic acid, succinic acid, glutaric acid, adipic acid, pimelic acid, suberic acid, azelaic acid, sebacic acid, fumaric acid, maleic acid, tartaric acid, malic acid, maleic acid, citric acid, aconitic acid, and mixtures thereof.
Anhydrides of polycarboxylic and monocarboxylic acids also are organic acids useful in the present compositions. Preferred anhydrides are anhydrides of polycarboxylic acids. At least a portion of the anhydride is hydrolyzed to a carboxylic acid because of the pH of the composition. It is envisioned that an anhydride can be slowly hydrolyzed on a surface contacted by the composition, and thereby assist in providing a persistent antiviral activity.
In a third embodiment, the organic acid comprises a polymeric carboxylic acid, a polymeric sulfonic acid, a sulfated polymer, a polymeric phosphoric acid, or mixtures thereof. The polymeric acid has a molecular weight of about 500 g/mol to 10,000,000 g/mol, and includes homopolymers, copolymers, and mixtures thereof. The polymeric acid preferably is capable of forming a substantive film on a skin surface and has a pKa less than about 6, preferably less than about 5.5, and a glass transition temperature, Tg, of less than about 25° C., preferably less than about 20° C., and more preferably less than about 15° C. The glass transition temperature is the temperature at which an amorphous material, such as a polymer, changes from a brittle vitreous state to a plastic state. The Tg of a polymer is readily determined by persons skilled in the art using standard techniques.
The polymeric acids are uncrosslinked or only very minimally crosslinked. The polymeric acids therefore are water soluble or at least water dispersible. The polymeric acids typically are prepared from ethylenically unsaturated monomers having at least one hydrophilic moiety, such as carboxyl, carboxylic acid anhydride, sulfonic acid, and sulfate. The polymeric acid can contain a comonomer, such as styrene or an alkene, to increase the hydrophobicity of the polymeric acid.
Examples of monomers used to prepare the polymeric organic acid include, but are not limited to:
(a) Carboxyl group-containing monomers, e.g., monoethylenically unsaturated mono- or polycarboxylic acids, such as acrylic acid, methacrylic acid, maleic acid, fumaric acid, crotonic acid, sorbic acid, itaconic acid, ethacrylic acid, α-chloroacrylic acid, α-cyanoacrylic acid, β-methlacrylic acid (crotonic acid), α-phenylacrylic acid, β-acryloxypropionic acid, sorbic acid, α-chlorosorbic acid, angelic acid, cinnamic acid, p-chlorocinnamic acid, β-stearylacrylic acid, citraconic acid, mesaconic acid, glutaconic acid, aconitic acid, tricarboxyethylene, and cinnamic acid;
(b) Carboxylic acid anhydride group-containing monomers, e.g., monoethylenically unsaturated polycarboxylic acid anhydrides, such as maleic anhydride; and
(c) Sulfonic acid group-containing monomers, e.g., aliphatic or aromatic vinyl sulfonic acids, such as vinylsulfonic acid, allylsulfonic acid, vinyltoluenesulfonic acid, styrenesulfonic acid, sulfoethyl (meth)acrylate, 2-acrylamido-2-methylpropane sulfonic acid, sulfopropyl (meth)acrylate, and 2-hydroxy-3-(meth)acryloxy propyl sulfonic acid.
The polymeric acid can contain other copolymerizable units, i.e., other monoethylenically unsaturated comonomers, well known in the art, as long as the polymer is substantially, i.e., at least 10%, and preferably at least 25%, acid group containing monomer units. To achieve the full advantage of the present invention, the polymeric acid contains at least 50%, and more preferably, at least 75%, and up to 100%, acid group containing monomer units. The other copolymerizable units, for example, can be styrene, an alkyl acrylate, or an alkyl methacrylate. The polymeric acid also can be partially neutralized, which assists dispersion of the polymeric acid into a composition. However, a sufficient number of the acid groups remain unneutralized to reduce surface pH and impart a persistent antiviral activity.
A polymeric acid assists in forming a film or layer of residual organic acid, or other skin pH-reducing compound, on the surface, and further assists in forming a more continuous layer of residual organic acid on the surface. A polymeric acid typically is used in conjunction with a monocarboxylic acid and/or a polycarboxylic acid, or other surface pH-reducing compound.
One preferred polymeric acid is a polyacrylic acid, either a homopolymer or a copolymer, for example, a copolymer of acrylic acid and an alkyl acrylate and/or alkyl methacrylate. Another preferred polymeric acid is a homopolymer or a copolymer of methacrylic acid.
Exemplary polymeric acids useful in the present invention include, but are not limited to:
In a preferred embodiment of the present invention, the organic acid comprises one or more polycarboxylic acid, e.g., citric acid, malic acid, tartaric acid, or a mixture of any two or all three of these acids, and a polymeric acid containing a plurality of carboxyl groups, for example, homopolymers and copolymers of acrylic acid or methacrylic acid.
The present method also can utilize an inorganic acid that is noncorrosive to the surface, in lieu of or together with an organic acid. Preferably, the inorganic acid is substantive to the surface to which it is applied. Like the organic acid, an inorganic acid typically is present in a composition for application to the surface in an amount of about 0.05% to about 15%, and preferably about 0.1% to about 10%, by weight of the composition. To achieve the full advantage of the present invention, the inorganic acid is present in an amount of about 0.15% to about 5%, by weight of the composition.
The inorganic acid has a pKa at 25° C. of less than 6, and preferably less than 5.5. To achieve the full advantage of the present invention, the inorganic acid has a pKa of 25° C. of less than 5. The identity of the inorganic acid is not limited, but the inorganic acid must possess sufficient acidity to lower a surface pH to less than about 4 without adversely effecting the surface, e.g., corrosion of an inanimate surface or irritation of an animate surface. Examples of inorganic acids include, but are not limited to, phosphoric acid, pyrophosphoric acid, polyphosphoric acid, phosphorous acid, and mixtures thereof, and similar noncorrosive inorganic acids.
An inorganic salt comprising a cation having a valence of 2, 3, or 4 and a counterion capable of lowering a surface pH, such as a skin pH, to less than about 4 can be used in lieu of, or together with, an organic acid and/or an inorganic acid. The inorganic salt, alone or in combination with the organic acid and/or inorganic acid, is present in a sufficient amount to control and inactivate viruses on a surface contacted in accordance with the present invention. Like the organic acid and inorganic acid, the inorganic salt provides a rapid control of acid-labile viruses, and provides a persistent viral control, by reducing the surface pH to less than about 4.
A cation of the inorganic salt has a valence of 2, 3, or 4, and can be, for example, magnesium, calcium, barium, aluminum, iron, cobalt, nickel, copper, zinc, zirconium, and tin. Preferred cations include, for example, zinc, aluminum, and copper.
Anions of the inorganic salt include, but are not limited to, bisulfate, sulfate, dihydrogen phosphate, monohydrogen phosphate, halides, such as chloride, iodide, and bromide, and nitrate. Preferred inorganic salts include chlorides and dihydrogen phosphates.
An inorganic salt is used in accordance with the present method in an amount of about 0.1% to about 5%, and preferably about 0.2% to about 2%, by weight of a composition. To achieve the full advantage of the present invention, the inorganic salt is applied to a surface as an aqueous solution containing about 0.3% to about 1% of an inorganic salt, by weight of the composition.
In one nonlimiting embodiment, the inorganic salt comprises a divalent zinc salt. A divalent zinc salt is described in detail herein, but it should be understood that similar polyvalent metal salts similarly can be used in accordance with the present method. In particular, divalent zinc salts useful in the present invention can have an organic or an inorganic counterion. In preferred embodiments, the divalent zinc ion, or any other useful cation, is applied in an unchelated or uncomplexed form, which allows the cation to more effectively contact, and potentially deposit, on the surface to assist in an effective and persistent control of microbes.
In some embodiments, however, an organic counterion complexes with the divalent zinc ion, i.e., Zn+2. Such embodiments are useful as long as the counterion lowers skin pH to less than about 4, and preferably the complexed Zn+2 has a sufficient equilibrium amount of uncomplexed Zn+2 help effectively control microbes on the skin.
A preferred divalent zinc salt, or other useful inorganic salt, has a water solubility of at least about 0.1 g (grams) per 100 ml (milliliters) of water at 25° C., and preferably about 0.25 g/100 ml of water at 25° C. Water-insoluble forms of zinc, e.g., zinc oxide, are not useful because the counterion is incapable of lowering skin pH and the zinc ion is essentially unavailable to assist in controlling microbes on the skin.
In most preferred embodiments, the divalent zinc salt, or other useful inorganic salt, is water soluble, but resists rinsing from the surface, and especially skin, to provide a persistent virucidal efficacy. Therefore, in most preferred embodiments, the counterion effectively lowers surface pH for about four hours or more and the divalent zinc or other cation is substantive to the surface, regardless of whether the aqueous solution containing the inorganic salt is rinsed from the surface after application, or is allowed to remain on the surface after application.
Although prior compositions including zinc salts addressed the ability of zinc ions to disrupt viral replication when the virus enters the epithelial cells of the nasal, oral, and pharyngeal mucosa, thus shortening the duration of the common cold, the present invention is directed to the surprising discovery that suitable inorganic salts, including zinc salts, provide unexpected benefits in protecting individuals from rhinoviral infection when applied to a surface, especially the hands and food contact surfaces. The benefit of preventing a viral infection therefore provides a level of protection greater than simply shortening the duration of infection.
Zinc salts useful in a present antimicrobial composition include, but are not limited to, divalent zinc salts having a counterion selected from the group consisting of gluconate, acetate, chloride, bromide, citrate, formate, glycerophosphate, iodide, lactate, salicylate, tartrate, and mixtures thereof.
An aluminum, zirconium, or aluminum-zirconium complex can be used in lieu of, or together with, an organic acid, an inorganic acid, and/or an inorganic salt. Such a complex, alone or in combination with an organic acid, an inorganic acid, and/or an inorganic salt, is applied to a surface in a sufficient amount to reduce skin pH to less than about 4, and thereby control and inactivate viruses on the surface. Like the organic acid, the inorganic acid, and the inorganic salt, these complexes provide a rapid control of acid-labile viruses, and can provide a persistent virus control for about four hours or more after application to a surface.
The aluminum, zirconium, and aluminum-zirconium complexes typically are polymeric in nature, contain hydroxyl moieties, and have an anion such as, but not limited to sulfate, chloride, chlorohydroxide, alumformate, lactate, benzyl sulfonate, or phenyl sulfonate. Exemplary classes of useful complexes include, but are not limited to, aluminum hydroxyhalides, zirconyl oxyhalides, zirconyl hydroxyhalides, and mixtures thereof. These complexes typically are acidic in nature, thereby providing a composition having a pH less than about 5 and typically having a pH of about 2 to about 4.5, and preferably about 3 to about 4.5. Accordingly, the complexes are capable of lowering skin pH to less than about 4.
Exemplary aluminum compounds include aluminum chloride and the aluminum hydroxyhalides having the general formula Al2(OH)xQy.XH2O, wherein Q is chlorine, bromine, or iodine; x is about 2 to about 5; x+y is about 6, wherein x and y are not necessarily integers; and X is about 1 to about 6. Exemplary zirconium compounds include zirconium oxy salts and zirconium hydroxy salts, also referred to as zirconyl salts and zirconyl hydroxy salts, and represented by the general empirical formula ZrO(OH)2-nz-Lz, wherein z varies from about 0.9 to about 2 and is not necessarily an integer; n is the valence of L; 2-nz is greater than or equal to 0; and L is selected from the group consisting of halides, nitrate, sulfamate, sulfate, and mixtures thereof.
Exemplary complexes, therefore, include, but are not limited to, aluminum chlorohydrate, aluminum-zirconium tetrachlorohydrate, an aluminum-zirconium polychlorohydrate complexed with glycine, aluminum-zirconium trichlorohydrate, aluminum-zirconium octachlorohydrate, aluminum sesquichlorohydrate, aluminum sesquichlorohydrex PG, aluminum chlorohydrex PEG, aluminum zirconium octachlorohydrex glycine complex, aluminum zirconium pentachlorohydrex glycine complex, aluminum zirconium tetrachlorohydrex glycine complex, aluminum zirconium trichlorohydrex glycine complex, aluminum chlorohydrex PG, zirconium chlorohydrate, aluminum dichlorohydrate, aluminum dichlorohydrex PEG, aluminum dichlorohydrex PG, aluminum sesquichlorohydrex PG, aluminum chloride, aluminum zirconium pentachlorohydrate, and mixtures thereof. Numerous other useful compounds are listed in WO 91/19222 and in the CTFA Cosmetic Ingredient Handbook, The Cosmetic, Toiletry and Fragrance Association, Inc., Washington, D.C., p. 56, 1988, hereinafter the CTFA Handbook, incorporated herein by reference.
Preferred compounds are the aluminum-zirconium chlorides complexed with an amino acid, like glycine, and the aluminum chlorohydrates. Preferred aluminum-zirconium chloride glycine complexes have an aluminum (Al) to zirconium (Zr) ratio of about 1.67 to about 12.5, and a total metal (Al+Zr) to chlorine ratio (metal to chlorine) of about 0.73 to about 1.93.
Typically, the present method is performed by incorporating an organic acid, inorganic acid, inorganic salt, zinc and/or aluminum complex, or mixtures thereof into a composition, then applying the composition to a surface. The carrier for the organic acid, inorganic acid, inorganic salt, and zinc and/or aluminum complex in such a composition comprises water. The composition can be a rinse-off or leave-on composition, as long as the surface contacted has a pH of less than about 4.
An antimicrobial composition of the present invention also can contain optional ingredients well known to persons skilled in the art. The particular optional ingredients and amounts that can be present in the composition are discussed hereafter.
The optional ingredients are present in a sufficient amount to perform their intended function and not adversely affect the antimicrobial efficacy of the composition, and in particular not adversely affect the synergistic effect provided by an optional disinfecting alcohol and organic acid, or a layer or film formed on a treated surface by the nonvolatile components of the composition. Optional ingredients typically are present, individually or collectively, from 0% to about 50%, by weight of the composition.
Classes of optional ingredients include, but are not limited to, hydrotropes, polyhydric solvents, disinfecting alcohols, gelling agents, active antimicrobial agents, surfactants, dyes, fragrances, pH adjusters, thickeners, viscosity modifiers, foam stabilizers, chelating agents, skin conditioners, emollients, preservatives, buffering agents, antioxidants, chelating agents, opacifiers, foam enhancers, and similar classes of optional ingredients known to persons skilled in the art.
The pH of a composition for lowering skin pH preferably is less than about 5, and preferably less than about 4.5. To achieve the full advantage of the present invention, the pH is less than about 4. Typically, the pH of a composition for lowering skin pH is about 2 to less than about 5, and preferably about 2.5 to about 4.5.
An antimicrobial agent can be present, if at all, in a composition for lowering surface pH in an amount of 0.1% to about 5%, and preferably about 0.1% to about 2%, and more preferably, about 0.3% to about 1%, by weight of the composition.
An optional active antimicrobial agent can be, for example, a bisguanidine (e.g., chlorhexidine digluconate), diphenyl compounds, benzyl alcohols, trihalocarbanilides, quaternary ammonium compounds, ethoxylated phenols, a peroxide, like hydrogen peroxide or benzoyl peroxide, and phenolic compounds, such as halo-substituted phenolic compounds, like PCMX (i.e., p-chloro-m-xylenol) and triclosan (i.e., 2,4,4′-trichloro-2′-hydroxydiphenylether). Preferred optional antibacterial agents are the phenolic and diphenyl compounds exemplified as follows.
Optional antimicrobial agents useful in the present invention are exemplified by the following classes of compounds used alone or in combination:
(1) Phenolic Antimicrobial Agents
(a) 2-Hydroxydiphenyl Compounds
wherein Y is chlorine or bromine, Z is SO3H, NO2, or C1-C4 alkyl, r is 0 to 3, o is 0 to 3, p is 0 or 1, m is 0 or 1, and n is 0 or 1.
In preferred embodiments, Y is chlorine or bromine, m is 0, n is 0 or 1, o is 1 or 2, r is 1 or 2, and p is 0.
In especially preferred embodiments, Y is chlorine, m is 0, n is 0, o is 1, r is 2, and p is 0.
A particularly useful 2-hydroxydiphenyl compound has a structure:
having the adopted name, triclosan, and available commercially under the tradename IRGASAN DP300, from Ciba Specialty Chemicals Corp., Greensboro, N.C. Another useful 2-hydroxydiphenyl compound is 2,2′-dihydroxy-5,5′-dibromo-diphenyl ether.
(b) Phenol Derivatives
wherein R1 is hydro, hydroxy, C1-C4 alkyl, chloro, nitro, phenyl, or benzyl; R2 is hydro, hydroxy, C1-C6 alkyl, or halo; R3 is hydro, C1-C6 alkyl, hydroxy, chloro, nitro, or a sulfur in the form of an alkali metal salt or ammonium salt; R4 is hydro or methyl; and R5 is hydro or nitro. Halo is bromo or, preferably, chloro.
Specific examples of phenol derivatives include, but are not limited to, chlorophenols (o-, m-, p-), 2,4-dichlorophenol, p-nitrophenol, picric acid, xylenol, p-chloro-m-xylenol, cresols (o-, m-, p-), p-chloro-m-cresol, pyrocatechol, resorcinol, 4-n-hexylresorcinol, pyrogallol, phloroglucin, carvacrol, thymol, p-chlorothymol, o-phenylphenol, o-benzylphenol, p-chloro-o-benzylphenol, phenol, 4-ethylphenol, and 4-phenolsulfonic acid. Other phenol derivatives are listed in U.S. Pat. No. 6,436,885, incorporated herein by reference.
(c) Diphenyl Compounds
wherein X is sulfur or a methylene group, R6 and R′6 are hydroxy, and R7, R′7, R8, R′8, R9, R′9, R10, and R′10, independent of one another, are hydro or halo. Specific, nonlimiting examples of diphenyl compounds are hexachlorophene, tetrachlorophene, dichlorophene, 2,3-dihydroxy-5,5′-dichlorodiphenyl sulfide, 2,2′-dihydroxy-3,3′,5,5′-tetrachlorodiphenyl sulfide, 2,2′-dihydroxy-3,5′,5,5′,6,6′-hexachlorodiphenyl sulfide, and 3,3′-dibromo-5,5′-dichloro-2,2′-dihydroxydiphenylamine. Other diphenyl compounds are listed in U.S. Pat. No. 6,436,885, incorporated herein by reference.
(2) Quaternary Ammonium Antimicrobial Agents
Useful quaternary ammonium antibacterial agents have a general structural formula:
wherein at least one of R11, R12, R13, and R14 is an alkyl, aryl, or alkaryl substituent containing 6 to 26 carbon atoms. Alternatively, any two of the R substituents can be taken together, with the nitrogen atom, to form a five- or six-membered aliphatic or aromatic ring. Preferably, the entire ammonium cation portion of the antibacterial agent has a molecular weight of at least 165.
The substituents R11, R12, R13, and R14 can be straight chained or can be branched, but preferably are straight chained, and can include one or more amide, ether, or ester linkage. In particular, at least one substituent is C6-C26alkyl, C6-C26alkoxyaryl, C6-C26alkaryl, halogen-substituted C6-C26alkaryl, C6-C26alkylphenoxyalkyl, and the like. The remaining substituents on the quaternary nitrogen atom other than the above-mentioned substituent typically contain no more than 12 carbon atoms. In addition, the nitrogen atom of the quaternary ammonium antibacterial agent can be present in a ring system, either aliphatic, e.g., piperidinyl, or aromatic, e.g., pyridinyl. The anion X can be any salt-forming anion which renders the quaternary ammonium compound water soluble. Anions include, but are not limited to, a halide, for example, chloride, bromide, or iodide, methosulfate, and ethosulfate.
Preferred quaternary ammonium antimicrobial agents have a structural formula:
wherein R12 and R13, independently, are C8-C12alkyl, or R12 is C12-C16alkyl, C8-C18alkylethoxy, or C8-C18alkylphenylethoxy, and R13 is benzyl, and X is halo, methosulfate, ethosulfate, or p-toluenesulfonate. The alkyl groups R12 and R13 can be straight chained or branched, and preferably are linear.
The quaternary ammonium antimicrobial agent in a present composition can be a single quaternary ammonium compound, or a mixture of two or more quaternary ammonium compounds. Particularly useful quaternary ammonium antimicrobial agents include dialkyl(C8-C10) dimethyl ammonium chlorides (e.g., dioctyl dimethyl ammonium chloride), alkyl dimethyl benzyl ammonium chlorides (e.g., benzalkonium chloride and myristyl dimethylbenzyl ammonium chloride), alkyl methyl dodecyl benzyl ammonium chloride, methyl dodecyl xylene-bis-trimethyl ammonium chloride, benzethonium chloride, dialkyl methyl benzyl ammonium chloride, alkyl dimethyl ethyl ammonium bromide, and an alkyl tertiary amine. Polymeric quaternary ammonium compounds based on these monomeric structures also can be used in the present invention. One example of a polymeric quaternary ammonium compound is POLYQUAT®, e.g., a 2-butenyl dimethyl ammonium chloride polymer. The above quaternary ammonium compounds are available commercially under the tradenames BARDAC®, BTC®, HYAMINE®, BARQUAT®, and LONZABAC®, from suppliers such as Lonza, Inc., Fairlawn, N.J. and Stepan Co., Northfield, Ill.
Additional examples of quaternary ammonium antimicrobial agents include, but are not limited to, alkyl ammonium halides, such as cetyl trimethyl ammonium bromide; alkyl aryl ammonium halides, such as octadecyl dimethyl benzyl ammonium bromide; N-alkyl pyridinium halides, such as N-cetyl pyridinium bromide; and the like. Other suitable quaternary ammonium antimicrobial agents have amide, ether, or ester moieties, such as octylphenoxyethoxy ethyl dimethyl benzyl ammonium chloride, N-(laurylcocoaminoformylmethyl)pyridinium chloride, and the like. Other classes of quaternary ammonium antimicrobial agents include those containing a substituted aromatic nucleus, for example, lauryloxyphenyl trimethyl ammonium chloride, cetylaminophenyl trimethyl ammonium methosulfate, dodecylphenyl trimethyl ammonium methosulfate, dodecylbenzyl trimethyl ammonium chloride, chlorinated dodecylbenzyl trimethyl ammonium chloride, and the like.
Specific quaternary ammonium antimicrobial agents include, but are not limited to, behenalkonium chloride, cetalkonium chloride, cetarylalkonium bromide, cetrimonium tosylate, cetyl pyridinium chloride, lauralkonium bromide, lauralkonium chloride, lapyrium chloride, lauryl pyridinium chloride, myristalkonium chloride, olealkonium chloride, and isostearyl ethyldimonium chloride. Preferred quaternary ammonium antimicrobial agents include benzalkonium chloride, benzethonium chloride, cetyl pyridinium bromide, and methylbenzethonium chloride.
(3) Anilide and Bisguanidine Antimicrobial Agents
Useful anilide and bisguanadine antimicrobial agents include, but are not limited to, triclocarban, carbanilide, salicylanilide, tribromosalan, tetrachlorosalicylanilide, fluorosalan, chlorhexidine gluconate, chlorhexidine hydrochloride, and mixtures thereof.
Compositions useful in the present method for lowering surface pH to produce a persistent control of bacteria and viruses also can contain, if at all, 10% to about 90%, by weight of an optional disinfecting alcohol. Preferred compositions contain an optional disinfecting alcohol in an amount of about 10% to about 70%, and more preferably about 20% to about 65%, by weight.
As used herein, the term “disinfecting alcohol” is a water-soluble alcohol containing one to six carbon atoms, i.e., a C1-6 alcohol. Disinfecting alcohols include, but are not limited to, methanol, ethanol, propanol, and isopropyl alcohol.
A surfactant can be included in a composition for lowering surface, and particularly skin, pH in an amount of 0% to about 15%, and typically 0.1% to about 10%, by weight, of the composition. More typically, if present at all, the composition contains about 0.2% to about 7%, by weight of the surfactant. The optional surfactant is stable at the pH of the composition and is compatible with the other ingredients present in the composition.
The surfactant can be an anionic surfactant, a cationic surfactant, a nonionic surfactant, or a compatible mixture of surfactants. The surfactant also can be an ampholytic or amphoteric surfactant, which have anionic or cationic properties depending upon the pH of the composition.
The compositions, therefore, can contain an anionic surfactant having a hydrophobic moiety, such as a carbon chain including about 8 to about 30 carbon atoms, and particularly about 12 to about 20 carbon atoms, and further has a hydrophilic moiety, such as sulfate, sulfonate, carbonate, phosphate, or carboxylate. Often, the hydrophobic carbon chain is etherified, such as with ethylene oxide or propylene oxide, to impart a particular physical property, such as increased water solubility or reduced surface tension to the anionic surfactant.
Suitable anionic surfactants include, but are not limited to, compounds in the classes known as alkyl sulfates, alkyl ether sulfates, alkyl ether sulfonates, sulfate esters of an alkylphenoxy polyoxyethylene ethanol, alpha-olefin sulfonates, beta-alkoxy alkane sulfonates, alkylaryl sulfonates, alkyl monoglyceride sulfates, alkyl monoglyceride sulfonates, alkyl carbonates, alkyl ether carboxylates, fatty acids, sulfosuccinates, sarcosinates, octoxynol or nonoxynol phosphates, taurates, fatty taurides, fatty acid amide polyoxyethylene sulfates, isethionates, acyl glutamates, alkyl sulfoacetates, acylated peptides, acyl lactylates, anionic fluoro surfactants, and mixtures thereof. Additional anionic surfactants are listed in McCutcheon's Emulsifiers and Detergents, 1993 Annuals, (hereafter McCutcheon's), McCutcheon Division, MC Publishing Co., Glen Rock, NJ, pp. 263-266, incorporated herein by reference. Numerous other anionic surfactants, and classes of anionic surfactants, are disclosed in U.S. Pat. No. 3,929,678 and U.S. Patent Publication No. 2002/0098159, each incorporated herein by reference.
Specific, nonlimiting classes of anionic surfactants useful in the present invention include, but are not limited to, a C8-C18 alkyl sulfonate, a C8-C18 alkyl sulfate, a C8-C18 fatty acid salt, a C8-C18 alkyl ether sulfate having one or two moles of ethoxylation, a C8-C18 alkamine oxide, a C8-C18 alkoyl sarcosinate, a C8-C1 sulfoacetate, a C9-C18 sulfosuccinate, a C8-C18 alkyl diphenyl oxide disulfonate, a C8-C18 alkyl carbonate, a C9-C18 alpha-olefin sulfonate, a methyl ester sulfonate, and mixtures thereof. The C8-C18 alkyl group contains eight to eighteen carbon atoms, and can be straight chain (e.g., lauryl) or branched (e.g., 2-ethylhexyl). The cation of the anionic surfactant can be an alkali metal (preferably sodium or potassium), ammonium, C1-C4 alkylammonium (mono-, di-, tri-), or C1-C3 alkanolammonium (mono-, di-, tri-). Lithium and alkaline earth cations (e.g., magnesium) can be used, but are not preferred.
Specific surfactants include, but are not limited to, lauryl sulfates, octyl sulfates, 2-ethylhexyl sulfates, decyl sulfates, tridecyl sulfates, cocoates, lauroyl sarcosinates, lauryl sulfosuccinates, linear C10 diphenyl oxide disulfonates, lauryl sulfosuccinates, lauryl ether sulfates (1 and 2 moles ethylene oxide), myristyl sulfates, oleates, stearates, tallates, ricinoleates, cetyl sulfates, and similar surfactants. Additional examples of surfactants can be found in “CTFA Cosmetic Ingredient Handbook,” J. M. Nikitakis, ed., The Cosmetic, Toiletry and Fragrance Association, Inc., Washington, D.C. (1988) (hereafter CTFA Handbook), pages 10-13, 42-46, and 87-94, incorporated herein by reference.
The compositions also can contain nonionic surfactants. Typically, a nonionic surfactant has a hydrophobic base, such as a long chain alkyl group or an alkylated aryl group, and a hydrophilic chain comprising a sufficient number (i.e., 1 to about 30) of ethoxy and/or
propoxy moieties. Examples of classes of nonionic surfactants include ethoxylated alkylphenols, ethoxylated and propoxylated fatty alcohols, polyethylene glycol ethers of methyl glucose, polyethylene glycol ethers of sorbitol, ethylene oxide-propylene oxide block copolymers, ethoxylated esters of fatty (C8-C18) acids, condensation products of ethylene oxide with long chain amines or amides, and mixtures thereof.
Exemplary nonionic surfactants include, but are not limited to, methyl gluceth-10, PEG-20 methyl glucose distearate, PEG-20 methyl glucose sesquistearate, C11-15 pareth-20, ceteth-8, ceteth-12, dodoxynol-12, laureth-15, PEG-20 castor oil, polysorbate 20, steareth-20, polyoxyethylene-10 cetyl ether, polyoxyethylene-10 stearyl ether, polyoxyethylene-20 cetyl ether, polyoxyethylene-10 oleyl ether, polyoxyethylene-20 oleyl ether, an ethoxylated nonylphenol, ethoxylated octylphenol, ethoxylated dodecylphenol, or ethoxylated fatty (C6-C22) alcohol, including 3 to 20 ethylene oxide moieties, polyoxyethylene-20 isohexadecyl ether, polyoxyethylene-23 glycerol laurate, polyoxyethylene-20 glyceryl stearate, PPG-10 methyl glucose ether, PPG-20 methyl glucose ether, polyoxyethylene-20 sorbitan monoesters, polyoxyethylene-80 castor oil, polyoxyethylene-15 tridecyl ether, polyoxyethylene-6 tridecyl ether, laureth-2, laureth-3, laureth-4, PEG-3 castor oil, PEG 600 dioleate, PEG 400 dioleate, and mixtures thereof.
Numerous other nonionic surfactants are disclosed in McCutcheon's, at pages 1-246 and 266-272; in the CTFA International Cosmetic Ingredient Dictionary, Fourth Ed., Cosmetic, Toiletry and Fragrance Association, Washington, D.C. (1991) (hereinafter the CTFA Dictionary) at pages 1-651; and in the CTFA Handbook, at pages 86-94, each incorporated herein by reference.
In addition to anionic and nonionic surfactants, cationic, ampholytic, and amphoteric surfactants can be used in the compositions. Useful cationic surfactants include those having a structural formula
wherein R15 is an alkyl group having about 12 to about 30 carbon atoms, or an aromatic, aryl, or alkaryl group having about 12 to about 30 carbon atoms; R16, R17, and R18, independently, are selected from the group consisting of hydrogen, an alkyl group having 1 to about 22 carbon atoms, or aromatic, aryl, or alkaryl groups having from about 12 to about 22 carbon atoms; and X is a compatible anion, preferably selected from the group consisting of chloride, bromide, iodide, acetate, phosphate, nitrate, sulfate, methyl sulfate, ethyl sulfate, tosylate, lactate, citrate, glycolate, and mixtures thereof. Additionally, the alkyl groups of R15, R16, R17, and R18 also can contain ester and/or ether linkages, or hydroxy or amino group substituents (e.g., the alkyl groups can contain polyethylene glycol and polypropylene glycol moieties).
Preferably, R15 is an alkyl group having about 12 to about 22 carbon atoms; R16 is H or an alkyl group having 1 to about 22 carbon atoms; and R17 and R18, independently are H or an alkyl group having 1 to about 3 carbon atoms. More preferably, R15 is an alkyl group having about 12 to about 22 carbon atoms, and R16, R17, and R18 are H or an alkyl group having 1 to about 3 carbon atoms.
Other useful cationic surfactants include amino-amides, wherein in the above structure R10 alternatively is R19CONH—(CH2)n, wherein R19 is an alkyl group having about 12 to about 22 carbon atoms, and n is an integer of 2 to 6, more preferably 2 to 4, and most preferably 2 to 3. Nonlimiting examples of these cationic surfactants include stearamidopropyl PG-dimonium chloride phosphate, behenamidopropyl PG dimonium chloride, stearamidopropyl ethyldimonium ethosulfate, stearamidopropyl dimethyl (myristyl acetate) ammonium chloride, stearamidopropyl dimethyl cetearyl ammonium tosylate, stearamidopropyl dimethyl ammonium chloride, stearamidopropyl dimethyl ammonium lactate, and mixtures thereof.
Nonlimiting examples of quaternary ammonium salt cationic surfactants include those selected from the group consisting of cetyl ammonium chloride, cetyl ammonium bromide, lauryl ammonium chloride, lauryl ammonium bromide, stearyl ammonium chloride, stearyl ammonium bromide, cetyl dimethyl ammonium chloride, cetyl dimethyl ammonium bromide, lauryl dimethyl ammonium chloride, lauryl dimethyl ammonium bromide, stearyl dimethyl ammonium chloride, stearyl dimethyl ammonium bromide, cetyl trimethyl ammonium chloride, cetyl trimethyl ammonium bromide, lauryl trimethyl ammonium chloride, lauryl trimethyl ammonium bromide, stearyl trimethyl ammonium chloride, stearyl trimethyl ammonium bromide, lauryl dimethyl ammonium chloride, stearyl dimethyl cetyl ditallow dimethyl ammonium chloride, dicetyl ammonium chloride, dicetyl ammonium bromide, dilauryl ammonium chloride, dilauryl ammonium bromide, distearyl ammonium chloride, distearyl ammonium bromide, dicetyl methyl ammonium chloride, dicetyl methyl ammonium bromide, dilauryl methyl ammonium chloride, dilauryl methyl ammonium bromide, distearyl methyl ammonium chloride, distearyl methyl ammonium bromide, and mixtures thereof.
Additional quaternary ammonium salts include those wherein the C12-C30 alkyl carbon chain is derived from a tallow fatty acid or from a coconut fatty acid. The term “tallow” refers to an alkyl group derived from tallow fatty acids (usually hydrogenated tallow fatty acids), which generally has mixtures of alkyl chains in the C16 to C18 range. The term “coconut” refers to an alkyl group derived from a coconut fatty acid, which generally have mixtures of alkyl chains in the C12 to C14 range. Examples of quaternary ammonium salts derived from these tallow and coconut sources include ditallow dimethyl ammonium chloride, ditallow dimethyl ammonium methyl sulfate, di(hydrogenated tallow) dimethyl ammonium chloride, di(hydrogenated tallow) dimethyl ammonium acetate, ditallow dipropyl ammonium phosphate, ditallow dimethyl ammonium nitrate, di(coconutalkyl)dimethyl ammonium chloride, di(coconutalkyl)dimethyl ammonium bromide, tallow ammonium chloride, coconut ammonium chloride, and mixtures thereof. An example of a quaternary ammonium compound having an alkyl group with an ester linkage is ditallowyl oxyethyl dimethyl ammonium chloride.
Ampholytic surfactants, i.e., amphoteric and zwitterionic surfactants, can be broadly described as derivatives of secondary and tertiary amines having straight chain or branched aliphatic radicals, and wherein one of the aliphatic substituents contains from about 8 to about 18 carbon atoms and at least one of the aliphatic substituents contains an anionic water-solubilizing group, e.g., carboxy, sulfonate, or sulfate.
More particularly, one class of ampholytic surfactants include sarcosinates and taurates having the general structural formula
wherein R20 is C11-C21 alkyl, R21 is hydrogen or C1-C2 alkyl, Y is CO2M or SO3M, M is an alkali metal, and n is a number 1 through 3.
Another class of ampholytic surfactants is the amide sulfosuccinates having the structural formula
The following classes of ampholytic surfactants also can be used:
Additional classes of ampholytic surfactants include the phosphobetaines and the phosphitaines.
Specific, nonlimiting examples of ampholytic surfactants useful in the present invention are sodium coconut N-methyl taurate, sodium oleyl N-methyl taurate, sodium tall oil acid N-methyl taurate, sodium palmitoyl N-methyl taurate, cocodimethylcarboxymethylbetaine, lauryldimethylcarboxymethylbetaine, lauryldimethylcarboxyethylbetaine, cetyldimethylcarboxymethylbetaine, lauryl-bis-(2-hydroxyethyl)carboxymethylbetaine, oleyldimethylgammacarboxypropylbetaine, lauryl-bis-(2-hydroxypropyl)-carboxyethylbetaine, cocoamidodimethylpropylsultaine, stearylamidodimethylpropylsultaine, laurylamido-bis-(2-hydroxyethyl)propylsultaine, disodium oleamide PEG-2 sulfosuccinate, TEA oleamido PEG-2 sulfosuccinate, disodium oleamide MEA sulfosuccinate, disodium oleamide MIPA sulfosuccinate, disodium ricinoleamide MEA sulfosuccinate, disodium undecylenamide MEA sulfosuccinate, disodium wheat germamido MEA sulfosuccinate, disodium wheat germamido PEG-2 sulfosuccinate, disodium isostearamideo MEA sulfosuccinate, cocoamphoglycinate, cocoamphocarboxyglycinate, lauroamphoglycinate, lauroamphocarboxyglycinate, capryloamphocarboxyglycinate, cocoamphopropionate, cocoamphocarboxypropionate, lauroamphocarboxypropionate, capryloamphocarboxypropionate, dihydroxyethyl tallow glycinate, cocamido disodium 3-hydroxypropyl phosphobetaine, lauric myristic amido disodium 3-hydroxypropyl phosphobetaine, lauric myristic amido glyceryl phosphobetaine, lauric myristic amido carboxy disodium 3-hydroxypropyl phosphobetaine, cocoamido propyl monosodium phosphitaine, lauric myristic amido propyl monosodium phosphitaine, and mixtures thereof.
Useful amphoteric surfactants also include the amine oxides. Amine oxides have a general structural formula wherein the hydrophilic portion contains a nitrogen atom that is bound to an oxygen atom with a semipolar bond.
R22, R23, and R24 can be a saturated or unsaturated, branched, or unbranched alkyl or alkenyl group having 1 to about 24 carbon atoms. Preferred amine oxides contain at least one R group that is an alkyl chain of 8 to 22 carbon atoms. Nonlimiting examples of amine oxides include alkyl dimethyl amine oxides, such as decylamine oxide, cocamine oxide, myristamine oxide, and palmitamine oxide. Also useful are the alkylaminopropylamine oxides, for example, coamidopropylamine oxide and stearamidopropylamine oxide.
Nonlimiting examples of preferred surfactants utilized in a composition include those selected from the group consisting of alkyl sulfates; alkyl ether sulfates; alkyl benzene sulfonates; alpha olefin sulfonates; primary or secondary alkyl sulfonates; alkyl phosphates; acyl taurates; alkyl sulfosuccinates; alkyl sulfoacetates; sulfonated fatty acids; alkyl trimethyl ammonium chlorides and bromides; dialkyl dimethyl ammonium chlorides and bromides; alkyl dimethyl amine oxides; alkylamidopropyl amine oxides; alkyl betaines; alkyl amidopropyl betaines; and mixtures thereof. More preferred surfactants include those selected from the group consisting of alkyl sulfates; alkyl ether sulfates; alkyl benzene sulfonates; alpha olefin sulfonates; primary or secondary alkyl sulfonates; alkyl dimethyl amine oxides; alkyl betaines; and mixtures thereof.
A hydrotrope, if present at all, is present in an amount of about 0.1% to about 30%, and preferably about 0.1% to about 20%, by weight of the composition. More preferably, a composition contains about 2% to about 15%, by weight of a hydrotrope.
A hydrotrope is a compound that has an ability to enhance the water solubility of other compounds. A hydrotrope utilized in the present invention lacks surfactant properties, and typically is a short-chain alkyl aryl sulfonate. Specific examples of hydrotropes include, but are not limited to, sodium cumene sulfonate, ammonium cumene sulfonate, ammonium xylene sulfonate, potassium toluene sulfonate, sodium toluene sulfonate, sodium xylene sulfonate, toluene sulfonic acid, and xylene sulfonic acid. Other useful hydrotropes include sodium polynaphthalene sulfonate, sodium polystyrene sulfonate, sodium methyl naphthalene sulfonate, sodium camphor sulfonate, and disodium succinate.
A polyhydric solvent, if present at all, is present in an amount of about 0.1% to about 50%, and preferably 5% to about 40%, by weight of the composition. To achieve the full advantage of the present invention, the polyhydric solvent is present in an amount of about 10% to about 30%, by weight, of the composition. In contrast to a disinfecting alcohol, a polyhydric solvent contributes minimally, if at all, to the efficacy of the composition.
The term “polyhydric solvent” as used herein is a water-soluble organic compound containing two to six, and typically two or three, hydroxyl groups. The term “water-soluble” means that the polyhydric solvent has a water solubility of at least 0.1 g of polyhydric solvent per 100 g of water at 25° C. There is no upper limit to the water solubility of the polyhydric solvent, e.g., the polyhydric solvent and water can be soluble in all proportions.
The term polyhydric solvent, therefore, encompasses water-soluble diols, triols, and polyols. Specific examples of hydric solvents include, but are not limited to, ethylene glycol, propylene glycol, glycerol, diethylene glycol, dipropylene glycol, tripropylene glycol, hexylene glycol, butylene glycol, 1,2,6-hexanetriol, sorbitol, PEG-4, and similar polyhydroxy compounds.
The compositions also can contain, if at all, about 0.1% to about 5%, by weight, and preferably 0.1% to about 3%, by weight of an optional gelling agent. More preferably, the compositions contain about 0.1% to about 2.5%, by weight of a gelling agent. The compositions contain a sufficient amount of gelling agent such that the composition is a viscous liquid, gel, or semisolid that can be easily applied to, and rubbed on, the skin or other surface. The optional gelling agent facilitates a uniform application of the composition onto a treated surface and helps provide a more continuous layer or film of nonvolatile composition ingredients on a treated surface. Persons skilled in the art are aware of the type and amount of gelling agent to include in the composition to provide the desired composition viscosity or consistency.
The term “gelling agent” as used here and hereafter refers to a compound capable of increasing the viscosity of a water-based composition, or capable of converting a water-based composition to a gel or semisolid. The gelling agent, therefore, can be organic in nature, for example, a natural gum or a synthetic polymer, or can be inorganic in nature.
The following are nonlimiting examples of gelling agents that can be used in the present invention. In particular, the following compounds, both organic and inorganic, act primarily by thickening or gelling the aqueous portion of the composition:
acacia, agar, algin, alginic acid, ammonium alginate, ammonium chloride, ammonium sulfate, amylopectin, attapulgite, bentonite, C9-15 alcohols, calcium acetate, calcium alginate, calcium carrageenan, calcium chloride, caprylic alcohol, carboxymethyl hydroxyethylcellulose, carboxymethyl hydroxypropyl guar, carrageenan, cellulose, cellulose gum, cetearyl alcohol, cetyl alcohol, corn starch, damar, dextrin, dibenzylidine sorbitol, ethylene dihydrogenated tallowamide, ethylene dioleamide, ethylene distearamide, fruit pectin, gelatin, guar gum, guar hydroxypropyltrimonium chloride, hectorite, hyaluronic acid, hydrated silica, hydroxybutyl methylcellulose, hydroxyethylcellulose, hydroxyethyl ethylcellulose, hydroxyethyl stearamide-MIPA, hydroxypropylcellulose, hydroxypropyl guar, hydroxypropyl methylcellulose, isocetyl alcohol, isostearyl alcohol, karaya gum, kelp, lauryl alcohol, locust bean gum, magnesium aluminum silicate, magnesium silicate, magnesium trisilicate, methoxy PEG-22/dodecyl glycol copolymer, methylcellulose, microcrystalline cellulose, montmorillonite, myristyl alcohol, oat flour, oleyl alcohol, palm kernel alcohol, pectin, PEG-2M, PEG-5M, polyvinyl alcohol, potassium alginate, potassium carrageenan, potassium chloride, potassium sulfate, potato starch, propylene glycol alginate, sodium carboxymethyl dextran, sodium carrageenan, sodium cellulose sulfate, sodium chloride, sodium silicoaluminate, sodium sulfate, stearalkonium bentonite, stearalkonium hectorite, stearyl alcohol, tallow alcohol, TEA-hydrochloride, tragacanth gum, tridecyl alcohol, tromethamine magnesium aluminum silicate, wheat flour, wheat starch, xanthan gum, polyvinylpyrrolidone and derivatives thereof, vinyl ether derivatives (methyl vinyl ether, ethyl vinyl ether, butyl vinyl ether, isobutyl vinyl ether, polymethyl vinyl ether/maleic acid), quaternized vinylpyrrolidone/quaternized dimethylamino ethyl pyrrolidone-based polymers and methacrylate copolymers, vinylcaprolactam/vinylpyrrolidone dimethylamino ethylmethacrylate polymers, vinylpyrrolidone/dimethyl amino ethylmethacrylate copolymers, acid stable and naturally occurring derivatives of guar and modified guar, modified or substituted xanthan, carboxypropyl cellulose, and mixtures thereof.
The following additional nonlimiting examples of gelling agents act primarily by thickening the nonaqueous portion of the composition:
abietyl alcohol, acrylinoleic acid, aluminum behenate, aluminum caprylate, aluminum dilinoleate, aluminum distearate, aluminum isostearates/laurates/palmitates or stearates, aluminum isostearates/myristates, aluminum isostearates/palmitates, aluminum isostearates/stearates, aluminum lanolate, aluminum myristates/palmitates, aluminum stearate, aluminum stearates, aluminum tristearate, beeswax, behenamide, behenyl alcohol, butadiene/acrylonitrile copolymer, a C29-70 acid, calcium behenate, calcium stearate, candelilla wax, carnauba, ceresin, cholesterol, cholesteryl hydroxystearate, coconut alcohol, copal, diglyceryl stearate malate, dihydroabietyl alcohol, dimethyl lauramine oleate, dodecanedioic acid/cetearyl alcohol/glycol copolymer, erucamide, ethylcellulose, glyceryl triacetyl hydroxystearate, glyceryl triacetyl ricinoleate, glycol dibehenate, glycol dioctanoate, glycol distearate, hexanediol distearate, hydrogenated C6-14 olefin polymers, hydrogenated castor oil, hydrogenated cottonseed oil, hydrogenated lard, hydrogenated menhaden oil, hydrogenated palm kernel glycerides, hydrogenated palm kernel oil, hydrogenated palm oil, hydrogenated polyisobutene, hydrogenated soybean oil, hydrogenated tallow amide, hydrogenated tallow glyceride, hydrogenated vegetable glyceride, hydrogenated vegetable glycerides, hydrogenated vegetable oil, hydroxypropylcellulose, isobutylene/isoprene copolymer, isocetyl stearoyl stearate, Japan wax, jojoba wax, lanolin alcohol, lauramide, methyl dehydroabietate, methyl hydrogenated rosinate, methyl rosinate, methylstyrene/vinyltoluene copolymer, microcrystalline wax, montan acid wax, montan wax, myristyleicosanol, myristyloctadecanol, octadecene/maleic anhydride copolymer, octyldodecyl stearoyl stearate, oleamide, oleostearine, ouricury wax, oxidized polyethylene, ozokerite, palm kernel alcohol, paraffin, pentaerythrityl hydrogenated rosinate, pentaerythrityl rosinate, pentaerythrityl tetraabietate, pentaerythrityl tetrabehenate, pentaerythrityl tetraoctanoate, pentaerythrityl tetraoleate, pentaerythrityl tetrastearate, phthalic anhydride/glycerin/glycidyl decanoate copolymer, phthalic/trimellitic/glycols copolymer, polybutene, polybutylene terephthalate, polydipentene, polyethylene, polyisobutene, polyisoprene, polyvinyl butyral, polyvinyl laurate, propylene glycol dicaprylate, propylene glycol dicocoate, propylene glycol diisononanoate, propylene glycol dilaurate, propylene glycol dipelargonate, propylene glycol distearate, propylene glycol diundecanoate, PVP/eicosene copolymer, PVP/hexadecene copolymer, rice bran wax, stearalkonium bentonite, stearalkonium hectorite, stearamide, stearamide DEA-distearate, stearamide DIBA-stearate, stearamide MEA-stearate, stearone, stearyl alcohol, stearyl erucamide, stearyl stearate, stearyl stearoyl stearate, synthetic beeswax, synthetic wax, trihydroxystearin, triisononanoin, triisostearin, triisostearyl trilinoleate, trilaurin, trilinoleic acid, trilinolein, trimyristin, triolein, tripalmitin, tristearin, zinc laurate, zinc myristate, zinc neodecanoate, zinc rosinate, zinc stearate, and mixtures thereof.
Exemplary gelling agents useful in the present invention include, but are not limited to,
Other specific classes of optional ingredients include alkanolamides as foam boosters and stabilizers; inorganic phosphates, sulfates, and carbonates as buffering agents; EDTA and phosphates as chelating agents; and acids and bases as pH adjusters.
Examples of preferred classes of optional basic pH adjusters are ammonia; mono-, di-, and tri-alkyl amines; mono-, di-, and tri-alkanolamines; alkali metal and alkaline earth metal hydroxides; and mixtures thereof. However, the identity of the basic pH adjuster is not limited, and any basic pH adjuster known in the art can be used. Specific, nonlimiting examples of basic pH adjusters are ammonia; sodium, potassium, and lithium hydroxide; monoethanolamine; triethylamine; isopropanolamine; diethanolamine; and triethanolamine.
Examples of preferred classes of optional acidic pH adjusters are the mineral acids. Nonlimiting examples of mineral acids are hydrochloric acid, nitric acid, phosphoric acid, and sulfuric acid. The identity of the acidic pH adjuster is not limited and any acidic pH adjuster known in the art, alone or in combination, can be used.
The composition also can contain a cosolvent or a clarifying agent, such as a polyethylene glycol having a molecular weight of up to about 4000, methylpropylene glycol, an oxygenated solvent of ethylene, propylene, or butylene, or mixtures thereof. The cosolvent or clarifying agent can be included as needed to impart stability and/or clarity to the composition and may be present in the residual film or layer of the composition on a treated surface.
An optional alkanolamide to provide composition thickening can be, but is not limited to, cocamide MEA, cocamide DEA, soyamide DEA, lauramide DEA, oleamide MIPA, stearamide MEA, myristamide MEA, lauramide MEA, capramide DEA, ricinoleamide DEA, myristamide DEA, stearamide DEA, oleylamide DEA, tallowamide DEA, lauramide MIPA, tallowamide MEA, isostearamide DEA, isostearamide MEA, and mixtures thereof. Alkanolamides are noncleansing surfactants and are added, if at all, in small amounts to thicken the composition.
The pH of a present antimicrobial composition is less than about 5, and preferably less than about 4.5 at 25° C. To achieve the full advantage of the present invention, the pH is less than about 4. Typically, the pH of a present composition is about 2 to less than about 5, and preferably about 2.5 to about 4.5.
The pH of the composition is sufficiently low such that at least a portion of an organic acid is in the protonated form. The organic acid then has the capability of lowering surface pH, such as skin pH, to provide an effective viral control, without irritating the skin. The organic acid also deposits on the skin to form a layer or film, and resists removal by rinsing, to provide a persistent antiviral effect.
To demonstrate the new and unexpected results provided by a method of the present invention, the following compositions were prepared and the ability of the method to control Gram positive and Gram negative bacteria, and to control rhinovirus, was determined. The weight percentage listed in each of the following compositions represents the actual, or active, weight amount of each ingredient present in a composition used in the present method of lowering skin pH. The compositions were prepared by blending the ingredients, as understood by those skilled in the art and as described below.
The following methods are used in the preparation and testing of the compositions:
a) Determination of Rapid Germicidal (Time Kill) Activity of Antibacterial Products. The activity of antibacterial compositions is measured by the time kill method, whereby the survival of challenged organisms exposed to an antibacterial test composition is determined as a function of time. In this test, a diluted aliquot of the composition is brought into contact with a known population of test bacteria for a specified time period at a specified temperature. The test composition is neutralized at the end of the time period, which arrests the antibacterial activity of the composition. The percent or, alternatively, log reduction from the original bacteria population is calculated.
In general, the time kill method is known to those skilled in the art.
The composition can be tested at any concentration up to 100%. The choice of which concentration to use is at the discretion of the investigator, and suitable concentrations are readily determined by those skilled in the art. For example, viscous samples usually are tested at 50% dilution, whereas nonviscous samples are not diluted. The test sample is placed in a sterile 250 ml beaker equipped with a magnetic stirring bar and the sample volume is brought to 100 ml, if needed, with sterile deionized water. All testing is performed in triplicate, the results are combined, and the average log reduction is reported.
The choice of contact time period also is at the discretion of the investigator. Any contact time period can be chosen. Typical contact times range from 15 seconds to 5 minutes, with 30 seconds and 1 minute being typical contact times. The contact temperature also can be any temperature, typically room temperature, i.e., about 25° C.
The bacterial suspension, or test inoculum, is prepared by growing a bacterial culture on any appropriate solid media (e.g., agar). The bacterial population then is washed from the agar with sterile physiological saline and the population of the bacterial suspension is adjusted to about 108 colony forming units per ml (cfu/ml).
The table below lists the test bacterial cultures used in the tests and includes the name of the bacteria, the ATCC (American Type Culture Collection) identification number, and the abbreviation for the name of the organism used hereafter. S. aureus is a Gram positive bacteria, whereas E. coli, K. pneum, and S. choler. are Gram negative bacteria.
Staphylococcus aureus
S. aureus
Escherichia coli
E. coli
Klebsiella pneumoniae
K. pneum.
Salmonella choleraesuis
S. choler.
The beaker containing the test composition is placed in a water bath (if constant temperature is desired), or placed on a magnetic stirrer (if ambient laboratory temperature is desired). The sample then is inoculated with 1.0 ml of the test bacteria suspension. The inoculum is stirred with the test composition for the predetermined contact time. When the contact time expires, 1.0 ml of the test composition/bacteria mixture is transferred into 9.0 ml of Neutralizer Solution. Decimal dilutions to a countable range then are made. The dilutions can differ for different organisms. Selected dilutions are plated in triplicate on TSA+ plates (TSA+ is Trypticase Soy Agar with Lecithin and Polysorbate 80). The plates then are incubated for 24±two hours, and the colonies are counted for the number of survivors and the calculation of percent or log reduction. The control count (numbers control) is determined by conducting the procedure as described above with the exception that deionized water is used in place of the test composition. The plate counts are converted to cfu/ml for the numbers control and samples, respectively, by standard microbiological methods.
The log reduction is calculated using the formula:
Log reduction=log10(numbers controlled)−log10(test sample survivors).
The following table correlates percent reduction in bacteria population to log reduction:
b) Antiviral Residual Efficacy Test
References: S. A. Sattar, Standard Test Method for Determining the Virus-Eliminating Effectiveness of Liquid Hygienic Handwash Agents Using the Fingerpads of Adult Volunteers, Annual Book of ASTM Standards. Designation E1838-96, incorporated herein by reference in its entirety, and referred to as “Sattar I”; and S. A. Sattar et al., Chemical Disinfection to Interrupt Transfer of Rhinovirus Type 14 from Environmental Surfaces to Hands, Applied and Environmental Microbiology, Vol. 59, No. 5, May, 1993, pp. 1579-1585, incorporated herein by reference in its entirety, and referred to as “Sattar II.”
The method used to determine the Antiviral Index of the present invention is a modification of that described in Sattar I, a test for the virucidal activity of liquid hand washes (rinse-off products). The method is modified in this case to provide reliable data for leave-on products.
The modifications from Sattar I include the product being delivered directly to skin as described below, virus inoculation of the fingerpads as described below, and viral recovery using ten-cycle washing. The inoculated skin site then is completely decontaminated by treating the area with 70% dilution of ethanol in water.
Procedure:
Ten-Minute Test:
Subjects (5 per test product) initially wash their hands with a nonmedicated soap, rinse the hands, and allow the hands to dry.
The hands then are treated with 70% ethanol and air dried.
Test product (1.0 ml) is applied to the hands, except for the thumbs, and allowed to dry.
About 10 minutes (±30 seconds) after product application, 10 μl of a Rhinovirus 14 suspension (ATCC VR-284, approximately 1×106 PFU (plaque-forming units)/ml) is topically applied using a micropipette to various sites on the hand within a designated skin surface area known as fingerpads. At this time, a solution of rhinovirus also is applied to the untreated thumb in a similar manner.
After a dry-down period of 7-10 minutes, the virus then is eluted from each of the various skin sites with 1 ml of eluent (Earle's Balanced Salt Solution (EBSS) with 25% Fetal Bovine Serum (FBS)+1% pen-strep-glutamate), washing 10 times per site.
The inoculated skin site then is completely decontaminated by rinsing the area with 70% ethanol. Viral titers are determined using standard techniques, i.e., plaque assays or TCID50 (Tissue Culture Infectious Dose).
One-Hour Test:
Subjects are allowed to resume normal activities (with the exception of washing their hands) between the 1-hour and 3-hour timepoints. After one hour, a rhinovirus suspension is applied to and eluted from designated sites on the fingerpads exactly as described in above for the 10-minute test.
A composition capable of lowering surface pH in accordance with the present invention was prepared by admixing the following ingredients at the indicated weight percentages until homogeneous.
The composition is applied to the surface, e.g., the skin, of an individual in a quantity sufficient to create a surface concentration of at least about 10 micrograms of citric acid per square centimeter of the surface. The surface pH is reduced from an ambient value of about 5 to 5.5 to an initial value after application of the composition of about 2 to 2.5. The surface is maintained at a pH of less than 3.5 for up to about five hours after application. The surface exhibits an excellent control of viruses and bacteria.
This example demonstrates the surprising and unexpected relationship between skin pH and antirhinoviral efficacy. While prior acidic compositions were applied to the skin of the user to provide antiviral, and particularly antirhinoviral, properties, it has been found that simply lowering the skin pH is not sufficient to assure antiviral efficacy. More specifically, to achieve a highly efficacious antiviral efficacy over an extended period of time, such as four hours, the pH of the skin must be maintained at less than 4 for the entire four hours.
In this example, antirhinoviral activity is assessed 5 minutes after application of an organic acid solution having a pH adjusted over a range of pH values in order to determine the effective pH limits of the compositions. Test solutions containing 1% citric acid and 1% malic acid, each by weight, in aqueous 10% ethanol solvent were prepared. The pH values of the solutions were adjusted by the addition of triethanolamine to provide compositions having a pH value indicated below:
The antirhinoviral efficacy of each solution was measured using the in vivo antirhinoviral fingerpad test procedure. The following table lists the composition tested, the skin pH after application of the test solution, the average log10 (viral titer inoculum applied to the fingers of volunteers), and the average log10 (viral titer recovered from the fingers). The test solution was applied to all fingers of the volunteers except the thumbs. The fingers then were allowed to dry for 5 minutes, and the rhinovirus inoculum was applied to all fingers. The thumbs serve as a negative control, and the inoculum was determined by the rhinovirus titer recovered from the thumbs. In this test, two volunteers were used for each pH tested. The skin pH reported is the average for the two volunteers.
This example clearly shows that a skin pH value of 5.6 or 4.7 is ineffective at eliminating rhinovirus, whereas a skin pH of 3.0 is highly effective at eliminating or essentially eliminating rhinovirus from human skin. An average log recovery of less than 1 indicates fewer than 1 virus particle remaining on average after the test, which also means that the virus level was below the limit of detection in the test.
The following compositions were prepared.
The samples were tested for antiviral activity against Rhinovirus 1A and Rotavirus Wa in a time kill suspension test. The following table summarizes the results of the test.
This example illustrates the synergistic antiviral effect provided by the combination of a disinfecting alcohol and an organic acid having a log P of less than one. Samples A and B show that a disinfecting alcohol alone does not provide an acceptable control of viruses. Sample E shows that salicylic acid dissolved in dipropylene glycol and water does not completely inactivate the tested virus serotypes. However, Samples C and D, which are compositions of the present invention, completely eliminate the tested virus serotypes.
The following antirhinoviral composition, which is capable of reducing skin pH, was prepared and applied to the fingerpads of human volunteers:
1)Acrylate/C10-30 Alkyl Acrylate Crosspolymer;
2)Preservative containing propylene glycol, diazolidinyl urea, methylparaben, and propylparaben.
In the test, composition 2D was applied to the fingerpads of all fingers, except the thumbs, of eight volunteers. The thumbs were control sites. The volunteers were divided into fours groups of two each. Each group I-IV then was challenged at a predetermined time with rhinovirus titer on all the fingerpads of each hand to determine the time-dependent efficacy of the test composition. At the time appropriate for each group, the skin pH of the fingerpads also was measured to determine the time course of skin pH in response to the test composition. The predetermined test time for rhinoviral challenge and skin pH measurement for each group I-IV were 5 minutes, 1 hour, two hours, and four hours, respectively. The following table shows the average log (rhinoviral titer inoculum), average skin pH, and average log (rhinoviral titer recovered) from the test fingerpads of the volunteers in the study, organized by group.
The data for each group (i.e., different time points) shows that the average recovered rhinoviral titer is less than 1 virus particle, or below the detection limit of the test. This data illustrates the efficacy of the present method after four hours and further demonstrates that a pH of less than about 4 is effective at completely eliminating a virus challenge. The combination of citric acid, malic acid, and polymeric acid (i.e., ULTREZ® 20) provided a residual barrier layer of organic acids on the fingerpads, which enhanced the persistent antiviral activity of the composition.
The clean fingerpads of test subjects were treated with the following compositions. Baseline skin pH readings were measured from the fingerpads prior to treatment with the compositions. Skin pH measurements also were taken immediately after the composition dried on the fingerpads, then again after four hours.
1)ETOH is ethanol
Four hours after treatment of the fingerpads with Samples A-G, Rhinovirus 39 at a titer of 1.3×103 pfu (plaque forming units) was applied to fingerpads. The virus was dried on the fingerpads for 10 minutes, then the fingerpads were rinsed with a viral recovery broth containing 75% EBSS and 25% FBS with 1× antibiotics. The sample was diluted serially in viral recovery broth and plated onto H1-HeLa cells. Titers were assayed as per the plaque assay. Complete inactivation of Rhinovirus 39, i.e., a greater than 3 log reduction, was achieved using the acid-containing compositions containing a mixture of two of citric acid, malic acid, and tartaric acid.
S. aureus
E. coli
1)Contact time on the skin
This example illustrates that compositions of the present invention also provide a rapid and broad spectrum antibacterial activity.
The clean fingerpads of test subjects were treated with the following composition. Baseline skin pH readings were measured from the fingerpads prior to treatment with the compositions. Skin pH measurements also were taken immediately after the composition dried on the fingerpads.
Immediately after treatment of the fingerpads with the composition, Rhinovirus 14 at a titer of 1.4×104 pfu (plaque forming units) was applied to the fingerpads. The virus was dried on the fingerpads for 10 minutes, then the fingerpads were rinsed with a viral recovery broth containing 75% EBSS and 25% FBS with 1× antibiotics. The sample was diluted serially in viral recovery broth and plated onto H1-HeLa cells. Titers were assayed as per the plaque assay. Complete inactivation of Rhinovirus 14 was achieved with the acid-containing composition resulting in a 4 log reduction.
The following compositions were prepared to test the effect of organic acids and organic acid blends on skin pH and antiviral efficacy.
The clean fingerpads of the test subjects were treated with Samples A-D. Baseline skin pH readings were measured from the fingerpads prior to treatment with a composition. Skin pH measurements also were taken immediately after the composition dried on the fingerpads, and again after two hours.
All Samples A-D suppressed skin pH to below 4 for two hours. The combination of citric acid and malic acid (Sample C) maintained a lower pH at two hours than the same acids used singly (Samples A and B). The 4% tartaric acid composition (Sample D) showed the greatest suppression of skin pH.
Two hours after treatment of the fingerpads with the solutions, Rhinovirus 39 at a titer of 4×104 pfu was applied to fingerpads. The virus was dried on the fingerpads for 10 minutes, then the fingerpads were rinsed with a viral recovery broth containing 75% EBSS and 25% FBS with 1× antibiotics. The sample was serially diluted in viral recovery broth and plated onto H1-HeLa cells. Titers were assayed as per the plaque assay. Complete inactivation of Rhinovirus 39 was achieved resulting in a greater than 3 log reduction.
The following examples illustrate that polymeric acids, and especially an acrylic acid homopolymer or copolymer, in the presence of alcohol impart antiviral efficacy. The polymeric acids have a low pH and good substantivity to skin, which effectively maintains a low skin pH over time, and helps provide a persistent antiviral efficacy. The polymeric acids also help provide an essentially continuous layer or film of an organic acid on treated surfaces, which in turn enhances the persistent antiviral activity of the composition.
A synergistic effect on the lowering of skin pH was demonstrated with using acrylic acid-based polymer in the presence of alcohol. However, an acrylic acid-based polymer in the absence of an alcohol did not maintain a reduced skin pH to the same degree over time. Importantly, skin pH reduction is less dependent on composition pH when a polymeric acid is used in conjunction with an alcohol. The synergy demonstrated between the polymeric acid and the alcohol was unexpected and is a novel way of providing the lowered skin pH that provides a desired antiviral efficacy.
A synergistic effect on a rapid and persistent antiviral activity also is demonstrated when an acrylic acid-based polymer is used in conjunction with polycarboxylic acids. It has been found that utilizing a low amount of a polymeric acid (e.g., about 0.1% to about 2%, by weight) together with a polycarboxylic acid, like citric acid, malic acid, tartaric acid, and mixtures thereof, enhances the antiviral activities of the polycarboxylic acids. This synergistic effect allows a reduction in the polycarboxylic acid concentration in an antiviral composition, without a concomitant decrease in antiviral efficacy. This reduction in polycarboxylic acid concentration improves composition mildness by reducing the irritation potential of the composition. It is theorized, but not relied upon herein, that the polymeric acid assists in forming a residual barrier film or layer of organic acids on a treated surface, which enhance the persistent antiviral activity of the composition.
A composition containing a polyacrylic acid (1% by wt), i.e., ULTREZ 20, available from Noveon Europe, was prepared in 70% aqueous ethanol and in water. Each composition (1.8 ml) was applied to the thumb, index, and middle fingers of a test subject. Skin pH readings were measured prior to treatment (baseline), immediately after the fingers were dry, and again after two hours. The average skin pH readings are summarized below.
The polyacrylic acid suppressed skin pH to about 4.5 initially, and skin pH remains under 5 after two hours. The composition with ethanol suppressed skin pH slightly lower (4.4) than the composition free of ethanol (4.5). This result suggests a synergistic effect on lowering skin pH when a polyacrylic acid is applied with ethanol.
Two hours after treatment of the fingerpads with the above compositions, Rhinovirus 39 was applied to the fingerpads that had been treated at a titer of 9.8×102 pfu. The virus was dried on the fingerpads for 10 minutes, then the fingerpads were rinsed with viral recovery broth. The broth was serially diluted in viral recovery broth and plated onto H1-HeLa cells. Titers were assayed as per the plaque assay. Both compositions reduced the viral titer. However, the composition containing ethanol exhibited slightly greater efficacy against Rhinovirus by reducing the titer by 1.8 log versus 1.5 log for the composition without ethanol.
This data illustrates that polyacrylic acid suppresses skin pH resulting in antiviral efficacy. The data also illustrates that polyacrylic acid and ethanol act synergistically to lower skin pH, thus resulting in a greater efficacy against rhinovirus.
To demonstrate this efficacy, the following eight compositions were prepared, wherein solutions containing a polyacrylic acid (with and without ethanol) were buffered to a pH of about 4.5, 5.0, 5.5, or 6.0.
The effect of the eight compositions on both skin pH and viral efficacy was tested. Each composition (1.8 ml) was applied to the thumb, index, and middle fingers of a test subject. Skin pH readings were measured prior to treatment (baseline), immediately after the product had dried, and again after two hours.
The skin pH data indicated that a polyacrylic acid and ethanol function synergistically to suppress skin pH because each composition containing ethanol in combination with the polyacrylic acid suppressed skin pH to a lower value than compositions free of ethanol. Compositions containing ethanol and polyacrylic acid lowered skin pH to between pH 4 and 5 independent of the solution pH. In contrast, compositions free of ethanol suppress the skin pH only to between pH 5-6 and the final skin pH is similar to the solution pH.
To test the viral efficacy of the above compositions, Rhinovirus 39 at a titer of 1.7×103 pfu was applied to the fingerpads after two hours. The virus dried for 10 minutes, eluted and diluted serially in viral recovery broth. Samples were plated on H1-HeLa cells, and virus titer was assayed as per the plaque assay method. The compositions containing ethanol in combination with polyacrylic acid had a greater than 2 log reduction in viral titers, whereas compositions free of ethanol exhibited a less than 1 log reduction in viral titers. Therefore, a synergism exists between polyacrylic acid and ethanol in reducing skin pH, which provides greater antiviral efficacy against rhinovirus. It is theorized, but not relied upon herein, that the ethanol helps provide a more continuous film or layer of the organic acid on the skin, for example, by reducing the surface tension of the composition for a more even and uniform application of the composition to a surface, and particularly skin.
The following compositions were prepared to further illustrate the antiviral efficacy provided by a polyacrylic acid.
1)CRODAFOS CS20 Acid is Ceteth-20 & Cetaryl Alcohol & Dicetyl Phosphate; and
2)NATROSOL 250 HHR CS is hydroxyethylcellulose.
Samples A-C (1.8 ml) were applied to the thumb, index, and middle fingers of clean hands. Skin pH readings were taken prior to treatment (baseline), immediately after the fingers were dry, and again after two hours for Samples A and B and after four hours for Sample C. The averages of the skin pH values are provided in the above table.
Sample A containing polyacrylic acid lowered the skin pH to the greatest extent with a final skin pH after two hours of pH 4.7. Neither Sample B nor Sample C lowered the skin pH below pH 5.0. This data indicates that polyacrylic acid has an ability to suppress skin pH and maintain a low skin pH for a least two hours.
The viral efficacy of Samples A-C against Rhinovirus 39 was also tested. A viral load of about 103 pfu was spread over the thumb, index, and middle fingers of each treated hand and allowed to dry for 10 minutes. The fingers then were rinsed with viral recovery broth and samples were serially diluted and plated on H1-HeLa cells. Viral titers were measured using the plaque assay. For both Samples B and C, 100% of the hands were positive for rhinovirus, which indicates little efficacy of these compositions against rhinovirus. In contrast, Sample A demonstrated a viral efficacy because only 63% of the hands were found positive for rhinovirus.
Example 9 demonstrated that a synergism exists between polyacrylic acid and ethanol, which results in suppression of skin pH and antiviral efficacy. The following compositions were prepared to examine the effectiveness of polycarboxylic acid blends and a single polycarboxylic acid composition, each in combination with polyacrylic acid and ethanol, on antiviral efficacy. A preferred antiviral composition contains the least amount of organic acid required to demonstrate a persistent antiviral efficacy.
The compositions were applied to the fingerpads of clean hands. After the indicated times, about 103 to 104 pfu of Rhinovirus 39 was applied to the hands and allowed to dry for 10 minutes. The virus was recovered by rinsing the hands with viral recovery broth. The samples then were diluted serially in viral recovery broth and plated on H1-HeLa cells. Viral titers were determined by plaque assay. The percentage of hands that were positive for rhinovirus is summarized below.
A composition containing 70% ethanol alone was not effective as an antiviral composition. Citric acid (1%) and malic acid (1%) lost effectiveness against rhinovirus after one hour because 100% of the hands were found to be positive for rhinovirus. In contrast, when a composition containing 1% citric and 1% malic acids are applied to the hands in combination with polyacrylic acid and 70% ethanol, no virus was detected on the hands after four hours. A single acid (4% citric acid) in combination with a polyacrylic acid and ethanol was less effective against rhinovirus because 91% of hands were found to be positive for rhinovirus after four hours.
This data demonstrates that using a polyacrylic acid and ethanol allows the use of a lower concentration of polycarboxylic acid to achieve a desired antiviral efficacy. This improvement is attributed, at least in part, to forming a residual film or layer of the organic acids on the skin.
The use of a polyacrylic acid and ethanol in a composition suppresses skin pH to a value below the solution pH, as demonstrated in Example 9. To test whether antiviral compositions containing citric acid, malic acid, polyacrylic acid, and ethanol can be buffered to a higher solution pH and still provide a skin pH at or below pH 4 to obtain a persistent antiviral activity, the following compositions were prepared.
The compositions (1.8 mL) were applied to the thumb, index, and middle fingers of clean hands. Skin pH readings were measured prior to treatment (baseline), immediately after the fingers were dry, and again after four hours. The average of the skin pH values are plotted above.
Initial skin pH of skin treated with Samples A-C were suppressed to between pH 2.9 and 3.6, wherein the lower the solution pH, the lower the initial skin pH. However, after four hours, the skin pH for all three compositions was about pH 3.7. Consistent with previous examples, solution pH did not predict subsequent skin pH.
The viral efficacy of Samples A-C against Rhinovirus 39 also was tested. A viral load of about 103 pfu was spread over the thumb, index, and middle fingers of each treated hand and allowed to dry for 10 minutes. The fingers then were rinsed with viral recovery broth and samples were diluted serially and plated on H1-HeLa cells. Viral titers were measured using the plaque assay. No virus was recovered from any of the hands indicating that all three Samples A-C have antiviral efficacy. This improvement is attributed, at least in part, to forming a residual film or layer of the organic acids on the skin.
This data demonstrates that when citric acid and malic acid are utilized in a composition in combination with a polyacrylic acid and ethanol, the pH of the solution can be buffered to a higher, e.g., milder and safer, pH for application to the skin, while still retaining an ability to suppress skin pH and exhibit antiviral activity. This result also is attributed, at least in part, to the residual layer or film of organic acid that remains on the skin after evaporation of volatile composition ingredients.
The following tests demonstrate that a composition of the present invention provides an essentially continuous barrier layer of organic acid on a treated surface. In particular, the following tests show that a present composition resists rinsing from a treated surface, e.g., at least 50% of the nonvolatile composition ingredients (including the organic acid) remains on a treated surface after three rinsings, as determined from NMR and IR spectra. In addition, an effective antiviral amount of the nonvolatile composition ingredients remains on a treated surface after 10 rinsings, also determined using NMR and IR spectra.
In the following tests, an aqueous composition containing, by weight, 2% malic acid, 2% citric acid, 1% polyacrylic acid, 62% ethanol, and 0.5% hydroxyethylcellulose as a gelling agent (Composition A) was compared to an aqueous composition, containing 2% malic acid, 2% citric acid, and 62% ethanol (Composition B). The compositions were applied to a glass surface to provide a film. From infrared (IR) and nuclear magnetic resonance (NMR) spectra of the film taken after each rinse, it was determined that Composition B was completely rinsed from the surface after one rinsing with water. Composition B therefore failed to exhibit water resistance and failed to provide a film or layer of nonvolatile composition ingredients on the surface.
In contrast, IR and NMR spectra showed that Composition A provided a rinse-resistant film or layer of composition ingredients on the treated surface. The amount of composition ingredients that remained on the treated surface was reduced over the first three rinsings, then resisted further removal from the treated surface in subsequent rinses. The IR and NMR spectra showed that detectable and effective amounts of the nonvolatile composition ingredients remained on the treated surface after 10 water rinses.
Another test was performed to measure the contact angle of water on a surface. “Contact angle” is a measure of the wetting ability of water on a surface. In this test, Compositions A and B were applied to a glass surface and allowed to dry. Contact angle then was measured for glass treated with Compositions A and B, both unrinsed and rinsed, using deionized water. The contact angle of bare, i.e., untreated, glass also was measured as a control. The following table summarizes the results of the contact angle test.
The contact angle data shows that Composition A modifies the glass surface and provides a persistent barrier film or layer on the glass surface. The data also shows that Composition B is rinsed from the surface because the contact angle after rinsing of Composition B is essentially the same as that of bare glass.
Another test was performed to demonstrate metal ion uptake by a residual film of Composition A. In this test, films of Composition A were formed on glass, dried at least 4 hours, then exposed to solutions having a 0.5 M concentration of metal ions. Samples then were analyzed by SEM scan. The data in the following table shows that a film resulting from Composition A effectively binds several types of metal ions. It is theorized, but not relied upon, that this is a surface phenomenon because no mechanism for transporting metal ions into the film is known.
Reflectance micrographs showing the surface coverage of Compositions A and B also were taken. The attached micrographs show that Composition A provides an essentially complete surface coverage, i.e., a more even coverage of Composition A on a treated surface, which provides an essentially continuous layer or film of nonvolatile composition ingredients on the surface. The attached micrographs are a digital conversion of reflectance values, which provide a direct correlation to surface coverage. The micrographs demonstrate that Composition A provides a film having improved adhesion, dispersion, and crystal formation compared to Composition B.
A time kill test was performed on additional bacteria and a fungus to demonstrate the broad spectrum efficacy of a composition of the present invention. In this test, the following antimicrobial composition was tested.
The above-composition was tested for an ability to control the following microorganisms under the following conditions:
Staphylococcus aureus ATCC 6538
Escherichia coli ATCC 11229
Listeria monocytogenes ATCC 7644
Enterobacter cloacae ATCC 13047
Candida albicans ATCC 10231
The test data summarized are below:
Staphylococcus aureus
Escherichia coli
Listeria monocytogenes
Enterobacter cloacae
Candida albicans
The data shows that a composition of present invention exhibits about a 4 to 5 log reduction at 15 and 30 seconds of exposure time against Staphylococcus aureus ATCC 6538, Escherichia coli ATCC 11229, Listeria monocytogenes ATCC 7644, Enterobacter cloacae ATCC 13047, and Candida albicans ATCC 10231.
The above data shows that a present antimicrobial composition containing an organic acid also is effective in controlling fungi, including yeasts and molds. Fungi control is important because fungi can cause a number of plant and animal diseases. For example, in humans, fungi cause ringworm, athlete's foot, and several additional serious diseases. Because fungi are more chemically and genetically similar to animals than other organisms, fungal diseases are very difficult to treat. Accordingly, prevention of fungal disease is desired. The prototype activity against fungi was examined using the yeast Candida albicans. The genus Candida contains a number of species, however, Candida albicans was tested because it is the most frequent cause of candidiasis. Candida albicans can be found in the alimentary tract, mouth, and vaginal area, and can cause diseases including oral candidiasis, also called thrush, vaginitis, alimentary candidiasis, and cutaneous and systemic candidiasis. In particular, a present invention is efficacious in controlling yeasts, such as Candida albicans, demonstrating a log reduction of at least 4 after a 15 second exposure time to a present antimicrobial composition.
The following are additional examples of compositions useful in the present method.
All compositions of Examples 14-17 are clear and colorless, and leave a slight residue when sprayed onto a countertop and allowed to dry.
A compound or composition capable of lowering surface pH and providing an antibacterial and antiviral efficacy can be formulated into a variety of product forms, including liquids, gels, semisolids, and solids. The liquid product form can be a solution, dispersion, emulsion, or a similar product form. Gel and semisolid product forms can be transparent or opaque, designed for application by stick dispenser or by the fingers, for example. Solid product forms can be a powder, flake, granule, tablet, pellet, lozenge, puck, briquette, brick, solid block, unit dose, or a similar solid product form known in the art. The present antimicrobial compositions can be manufactured as dilute ready-to-use compositions, or as concentrates that are diluted prior to use.
One particular product form is a liquid or solid composition disposed within a water-soluble packet. The packet is added to a proper amount of water, and the composition is released when the packet dissolves. The water-soluble packet typically comprises a polyvinylalcohol. One form of water-soluble packet is disclosed in U.S. Pat. No. 5,316,688, incorporated herein by reference. Numerous other water-soluble packets are known to person skilled in the art, for example, in U.S. Pat. Nos. 5,070,126; 6,608,121; and 6,787,512; U.S. Patent Publication No. 2002/0182348; WO 01/79417; and European Patent Nos. 0 444 230, 1 158 016, 1 180 536, and 1 251 147, each incorporated herein by reference. Capsules are another related and useful product form.
Another useful product form is a stable, solid block that can be added to water to provide a liquid composition for practicing the present methods. The block can be tablet, briquette, puck, or larger solid block, e.g., the block can weigh from less than one ounce to several pounds, depending on the end use application. Such blocks generally comprise a binding agent. One stable block is disclosed in U.S. Pat. No. 6,432,906, incorporated herein by reference.
Yet another product form is incorporation of the active compound or composition into an absorbent or adsorbent carrier, such as polymeric microparticles or inorganic particles. The loaded carrier can be used as is, or incorporated into other product forms, either liquid, gel, semisolid, or solid.
Still another product form is a web material or swab containing a compound or composition capable of lowering a surface pH. The compound or composition then can be applied to the skin by wiping the surface with the web material containing the compound or composition.
Another product form is an article, such as latex gloves, having the active compound or composition applied to, or imbedded into, the article. During use, the compound or composition imparts antiviral activity to the article itself and/or to a surface contacted by the article. Additional articles that can have an active compound or composition imbedded therein are plastic cups, food wraps, and plastic containers.
As discussed above, both animate and inanimate surfaces can be treated in accordance with the method of the present invention. A particularly important surface is mammalian skin, and especially human skin, to inactivate and interrupt the transmission of bacteria and viruses. However, the present method also is useful in treating other animate surfaces and inanimate surfaces of all types.
For example, a present compound or composition can be applied to food products, such as meat, poultry, seafood, fruits, and vegetables. The compositions are applied to the surfaces of food products to control microorganisms. Examples of microorganisms include pathogenic microorganisms that can cause illness (e.g., Listeria monocytogenes, enterohemorrhagic Escherichia coli, Salmonella, and the like) and spoilage organisms that can affect the taste, color, and/or smell of the final food product (e.g., Pseudomonas, Acinetobacter, Moraxella, Alcaligenes, Flavobacterium, Erwinia, and the like).
The compositions can be applied to any food product that is consumed by a human or an animal, including both food and beverages, and specifically meat, poultry, seafood, fruits, and vegetables. Some nonlimiting examples of meat products include muscle meat or any portion thereof of any animal including beef, pork, veal, buffalo, and lamb. Some nonlimiting examples of seafood include scallops, shrimp, crab, octopus, mussels, squid, and lobsters. Some nonlimiting examples of poultry include chicken, turkey, ostrich, game hen, squab, guinea fowl, pheasant, duck, goose, and emu. Some nonlimiting examples of fruits and vegetables include citrus fruits, tree fruits, tropical fruits, berries, lettuce, green beans, peas, carrots, tomatoes, mushrooms, potatoes, root vegetables, sprouts, seeds, nuts, animal feed, and grains such as corn, wheat, and oats.
The compositions can be applied to the surface of the food product in several ways including spraying, misting, rolling, and foaming the composition onto the food product, or immersing the food product in the composition. The composition can be applied by injection, such as in an injection solution, or the composition can be applied as a component of a marinade or tenderizer that is applied to the food product. The application of the composition can be combined with physical agitation, such as spraying with pressure, rubbing, or brushing. Application of the composition can be manual, or the composition can be applied in a spray booth. The spray can comprise of fog material delivered from a fogging apparatus as a dispersion of fog particles in a continuous atmosphere. The composition can be used on a food product once, then discarded, or the composition can be recycled.
The food product also can be immersed into a container containing the composition. The composition preferably is agitated to increase the efficacy of this solution and the speed in which the solution kills microorganisms attached to the food product.
In another embodiment of the present invention, the food product can be treated with a foaming version of the composition. The foam can be prepared by mixing a foaming surfactant with the composition at the time of use. The foaming surfactants can be nonionic, anionic, or cationic in nature.
In still another embodiment of the invention, the food product can be treated with a thickened or gelled composition. In the thickened or gelled state, the compositions remain in contact with the food product for longer periods of time, thus increasing the antimicrobial efficacy. The thickened or gelled composition also adheres to vertical surfaces.
The volume of composition per pound of foodstuff is an important parameter with respect to the antimicrobial efficacy of the compositions. Preferred volumes of the composition for treated poultry, fish, fruits, and vegetables and red meat pieces/trim are about 0.5 oz/lb to about 3.0 oz/lb, and more preferably, about 1.0 to about 2.0 oz/lb of foodstuff in dip and spray applications. For beef carcasses, the preferred volumes are about 0.5 to about 2.5 gallons per side of beef, and more preferably about 1.0 to about 2.0 gallons/side.
The treatment of food products with a disinfecting composition is described in greater detail in U.S. Pat. Nos. 5,389,390; 5,409,713; 6,063,425; 6,183,807; 6,113,963; 6,514,556; and 6,545,047, the disclosures of which are incorporated by reference herein in their entirety.
The compositions also can be applied to live animals, for example, as teat dips or hoof treatments. Teat dips are known as a method of reducing bovine mastitis in dairy herds. Mastitis is one of the most common and economically costly diseases confronting milk producers. Economic losses result from poor milk quality, lower milk production, and potential culling of chronically infected animals. The use of an antimicrobial composition both before and after milking has found great success in preventing mastitis. When a composition is used as a teat dip, it may be desirable to add additional ingredients that enhance the effectiveness of the composition or provide additional benefit, such as a dye to indicate that the composition has been properly applied.
The composition also can be used as a foot bath or hoof treatment to prevent the spread of diseases. For example, the composition can be formulated and applied such that farm workers walk through the composition and thereby prevent microorganisms on their boots from spreading. Alternatively, the composition can be used in such a way that animals walk through the composition, thereby preventing the spread of microorganisms, and also providing an opportunity to treat any infections on the hooves of the animals.
The present method also is useful to treat inanimate surfaces, both soft and hard. As used herein, the term “hard” refers to surfaces comprising refractory materials, such as glazed and unglazed tile, brick, porcelain, ceramics, metals, glass, and the like, and also includes wood and hard plastics, such as formica, polystyrenes, vinyls, acrylics, polyesters, and the like. A hard surface can be porous or nonporous. Methods of disinfecting hard surfaces are described in greater detail in U.S. Pat. Nos. 5,200,189; 5,314,687; and 5,718,910, each disclosure incorporated herein by reference.
The present method can be used to treat hard surfaces in processing facilities (such as dairy, brewing, and food processing facilities), healthcare facilities (such as hospitals, clinics, surgical centers, dental offices, and laboratories), long-term healthcare facilities (such as nursing homes), farms, cruise ships, hotels, airplanes, schools, and private homes.
The present method can be used to treat environmental hard surfaces such as floors, walls, ceilings, and drains. The method can be used to treat equipment such as food processing equipment, dairy processing equipment, brewery equipment, and the like. The compositions can be used to treat a variety of surfaces including food contact surfaces in food, dairy, and brewing facilities, such as countertops, furniture, sinks, and the like. The method further can be used to treat tools and instruments, such as medical tools and instruments, dental tools and instruments, as well as equipment used in the healthcare industries and institutional kitchens, e.g., meat slicers, cutting boards, knives, forks, spoons, wares (such as pots, pans, and dishes), cutting equipment, and the like.
Treatable inanimate surfaces include, but are not limited to, exposed environmental surfaces, such as tables, floors, walls, kitchenware (including pots, pans, knives, forks, spoons, plates), food cooking and preparation surfaces, including dishes and food preparation equipment, tanks, vats, lines, pumps, hoses, and other process equipment. One useful application of the composition is to dairy processing equipment, which is commonly made from glass or stainless steel. Dairy process equipment can be found in dairy farm installations and in dairy plant installations for the processing of milk, cheese, ice cream, and other dairy products.
In use, the compositions are applied to target animate and inanimate surfaces. The compositions can be applied by dipping a surface into the composition, soaking a surface in the composition, or spraying, wiping, foaming, misting, brushing, pod coating, rolling, and fogging the composition onto an animate or inanimate surface. The composition can be applied manually or using equipment such as a spray bottle or by machine, such as a spray machine, foam machine, and the like. The composition can also be used inside a machine, such as a warewashing machine or laundry machine. For household applications, hand-operated pump-type or pressurized aerosol sprayers can be used. The compositions also can be employed to coat or otherwise treat materials such as sponges, fibrous or nonfibrous web materials, swabs, flexible plastics, textiles, wood, and the like. Generally, the coating process is used to impart prolonged antiviral properties to a porous or nonporous surface by coating said surface with the composition.
The method of the present invention also can be used in the manufacture of beverages including fruit juice, malt beverages, bottled water products, teas, and soft drinks. The method can be used to treat pumps, lines, tanks, and mixing equipment used in the manufacture of such beverages. The method of the present invention also can be used to treat air filters.
The method of the present invention is useful in the treatment of medical carts, medical cages, and other medical instruments, devices, and equipment. Examples of medical apparatus treatable by the present method are disclosed in U.S. Pat. No. 6,632,291, incorporated herein by reference. The present method also is useful in treating utensil and chairs present in barber shops, and hair and nail salons. A further useful application is to treat coins, paper money, tokens, poker chips, and similar articles that are repeatedly handled by numerous individuals and can transmit viruses between individuals.
In addition to hard surfaces, the method also can be used to treat soft inanimate surfaces, like textiles, such as clothing, protective clothing, laboratory clothing, surgical clothing, patient clothing, carpets, bedding, towels, linens, and the like. The method also can be used to treat face masks, medical gowns, gloves, and related apparel utilized by medical and dental personnel.
The method of the present invention can be practiced using, for example, hand cleansers, surgical scrubs, body splashes, antiseptics, disinfectants, hand sanitizers, deodorants, and similar personal care products. Additional types of compositions that can be used in the present method include foamed compositions, such as creams, mousses, and the like, and compositions containing organic and inorganic filler materials, such as emulsions, lotions, creams, ointments, pastes, and the like. The method also can be practiced by incorporating a suitable compound or composition into a swab or a web material to provide a wiping article. The wiping article can be used to control microbes on animate or inanimate surfaces.
In one embodiment of the present invention, a person suffering from a rhinovirus cold, or who is likely to be exposed to other individuals suffering from rhinovirus colds, can apply a compound or composition capable of lowering skin pH to less than 4 to his or her hands. This application kills bacteria and inactivates rhinovirus particles present on the hands. The applied compound or composition, either rinsed off or allowed to remain on the hands, provides a persistent antiviral activity. Rhinovirus particles therefore are not transmitted to noninfected individuals via hand-to-hand transmission. The amount of the compound or composition applied, the frequency of application, and the period of use will vary depending upon the level of disinfection desired, e.g., the degree of microbial contamination.
The present method provides the advantages of a broad spectrum kill of Gram positive and Gram negative bacteria, and a viral control, in short contact times. The short contact time for a substantial log reduction of bacteria is important in view of the typical 15 to 60 second time frame used to cleanse and sanitize animate and inanimate surfaces. The method also imparts a persistent antiviral activity to the contacted surface, which is enhanced because of a residual barrier layer or film of composition ingredients that can remain on the surface after evaporation of the volatile components of the composition.
Obviously, many modifications and variations of the invention as hereinbefore set forth can be made without departing from the spirit and scope thereof, and, therefore, only such limitations should be imposed as are indicated by the appended claims.
This application claims the benefit of U.S. Provisional Patent Application No. 60/811,032, filed Jun. 5, 2006, and U.S. Provisional Patent Application No. 60/811,354, filed Jun. 6, 2006.
| Number | Date | Country | |
|---|---|---|---|
| 60811032 | Jun 2006 | US | |
| 60811354 | Jun 2006 | US |
