METHODS AND COMPOSITIONS FOR CONTROL OF CABBAGE LOOPER, Trichoplusia ni

Information

  • Patent Application
  • 20100160451
  • Publication Number
    20100160451
  • Date Filed
    November 19, 2009
    14 years ago
  • Date Published
    June 24, 2010
    13 years ago
Abstract
The invention provides in part dialkoxybenzene compounds for controlling infestation by a Trichoplusia ni, and methods thereof. The compounds include a compound of Formula I:
Description
FIELD OF INVENTION

The present invention relates to insect control. More specifically, the present invention relates to methods and compositions for control of the cabbage looper, Trichoplusia ni.


BACKGROUND OF THE INVENTION

The behavioral manipulation of insect pests for their management, as an alternative to broad-spectrum insecticides, has been investigated for many years.


In addition to the development of resistance against insecticides by the target organism, broad-spectrum insecticides also have negative impacts on natural enemies of the pest insect, on pollinators and on other non-target organisms. Therefore, there is an increased interest in the behavioral manipulation of insect pests for their management as an alternative to broad-spectrum insecticides. Of particular interest are compounds that do not exhibit substantial toxicity or demonstrate some degree of selectivity towards a pest insect and not towards natural enemies, pollinators or the environment. In practice, manipulation may be achieved through the use of stimuli that either enhance or inhibit a particular behavior and ultimately change its expression. Many natural plant defensive chemicals discourage insect herbivory, for example, by deterring feeding and oviposition or by impairing larval growth, rather than by killing insects.


The choice of a stimulus for behavioral manipulation is usually dependent upon a number of factors including accessibility, reproducibility, specificity and practicality (Foster and Marris 1997). Various short- or long-range stimuli, involved in behavioral manipulation of insects, are perceived through contact chemoreceptors or olfactory receptors, respectively. These stimuli can either stimulate feeding or oviposition, keeping the insect at the host plant; or inhibit those behaviors, resulting in the insect abandoning the plant. Examples of feeding stimulants often include carbohydrates, proteins, or fats (Ave 1995) that are ubiquitous in plants, whereas oviposition stimulants can be highly species-specific. Feeding stimulants can be used in conjunction with toxins in “attract and kill” strategies (Ave 1995), occasionally employed in crop protection. A deterrent can be applied to a host plant to prevent feeding or oviposition. Therefore, deterrents may have potential value in crop protection, in combination with other strategies such as “attract and kill” (Jermy 1965; Munakata 1970).


Insect feeding deterrents can be found among all the major classes of plant secondary metabolites—alkaloids, phenolics and terpenoids (Frazier 1986). Especially well studied in this group are the triterpenes such as the limonoids from the neem (Azadirachta indica) and chinaberry (Melia azedarach) trees and from Citrus species and the diterpenes including the clerodanes and the abietanes (Isman 2002). Apart from terpenes, another important class of compounds involved in defense of the plant against herbivores and pathogens, as well as in attracting pollinators, are the compounds derived from aromatic amino acids—phenylpropanoids (Wildman 2006).


Eugenol is a volatile member of the phenylpropanoid class of compounds from essential oils of many spices, particularly clove (Dewick 2002). Cloves are useful in the home as moth deterrents and the main odorant from cloves, eugenol, has been reported to be perceived as a long-range stimulus by several lepidopterans (Topazzini et al. 1990). One problem with phenylpropanoids such as eugenol and compounds with a cinnamyl framework is that they can produce toxic metabolites after benzylic/allylic oxidation by certain cytochrome P450 enzymes (Dewick 2002).


Several polyphenolic compounds are also known for their toxic/insecticidal effects (Kim and Ahn 2001; Schneider et al. 2000; Khambay et al. 1999; Harborne 1989). Flavonoids isolated from Annona squamosa (Kotkar et al. 2002), Ricinus communis (Upasani 2003) and Calotropis procera (Salunke et al. 2005), are toxic to the pulse beetle. Callosobruchus chinensis and R. communis also caused oviposition deterrent and ovicidal affects in addition to toxicity. Larvicidal activity of lignans, leptostachyol acetate and analogues from the roots of Phryma leptostachya have been reported against three mosquito species (Culex pipiens pallens, Aedes aegypti, and Ocheratatos togoi) (Park et al. 2005).


Compounds derived from aromatic amino acids, such as some phenolics, have been reported to be involved in defense of the plant against herbivores and pathogens, as well as in attracting pollinators. For example phenol derivatives such as guaiacol (1-hydroxy-2-methoxybenzene), 1,2-dimethoxybenzene, 1-ethoxy-2-methoxybenzene, 1-propoxy-2-methoxybenzene, eugenol and isoeugenol, occur in smoke (Guillen and Manzanos 2005; Murugan et al. 2006) and are reported to have insect-repellent and insecticidal activities (Murugan et al. 2006). Furthermore, smoke phenolics taste and smell pleasantly (to humans) (Guillen and Manzanos 2005) and may have antioxidant activity (Bortolomeazzi, et al. 2006). Eugenol (2-methoxy-4-(2-propenyl)phenol), is found in herbs (such as basil, Ocimum suave (Wild.)) and has been reported to have activity against grain beetles as a toxicant and deterrent (Obeng-Ofor and Reichmuth 1997). Other benzene derivatives, such as benzyl alcohol, benzonitrile, phenylethanol, 4-methyl phenol, 4-ethylphenol, 2-methylphenol and benzaldehyde are reported components of human odor that malaria mosquitoes respond to (Hallem et al. 2004; Meijerink et al. 2000).


Widely distributed, the cabbage looper Trichoplusia ni is considered an important field and greenhouse pest in vegetable crop production. This species is a generalist and attacks a variety of crops including lettuce, beets, turnip, spinach, brussel sprouts, peas, celery, tomatoes, rape, tobacco, certain ornamentals, many weedy plants, as well as cruciferous plants. Moths emerge in the spring and use two mate-finding strategies (Landolt and Heath 1990). One strategy involves male attraction to the female-produced sex pheromone which includes the major component Z-7-dodecenyl acetate (Berger 1966) and several other structurally related compounds (Bjostad et al. 1984). The other strategy involves female attraction to the male pheromone composed of the major component S-(+)-linalool, as well as p-cresol and m-cresol (Heath et al. 1992). The amount of pheromone released by the male has been reported to be affected by the cabbage odour.


The mated females deposit dome-shaped, pale green eggs singly on the host-plants, chiefly at night. After hatching, the destructive larval stage reaches full development in two to four weeks; pupation then occurs and in almost 10 days the new adults emerge. In general, the larval stages damage the crop. The first two larval stages feed on the lower side of the leaf, eating through the upper epidermis, leaving “windows” in the leaf. Older larvae chew larger holes in the leaves, often resulting in extensive damage to leaves. Although this pest generally damages leaves, damage has been reported on watermelon rinds and on flowers of various host plants. Three or more generations are generally produced each season, depending on the latitude (Davidson and Lyon 1979).


The loopers overwinter in the pupal stage, the pupae enclosed in flimsy silken cocoons attached to the food plants or to nearby objects. Cabbage loopers do not generally overwinter in Canada and migrate in from the south. However, they can overwinter in greenhouses.


Chemosensory input from contact chemosensilla present on the tarsi, antennae, and other parts of the body, such as the ovipositor, affects feeding and oviposition behaviors in cabbage looper as well as other phytophagous insects. Based on the sensory information received, an insect can chose a proper feeding or an oviposition site. Neonates of many species including cabbage looper are incapable of locating a new host and are dependent on the host plant location “skills” of their mothers (Feeny et al. 1983). Therefore, the site of emergence is of importance to the larvae of many lepidopteran species (Restraits 1966).



T. ni has developed resistance to a number of commercial insecticides, including early generation insecticides such as DDT, carbaryl, parathion, (McEwen 1956) as well as more modern insecticides such as methomyl and Bt (Bacillus thuringiensis toxin), a widely used benign and specific insecticide against moth pests that are in the larval stages (Wang et al. 2007).


SUMMARY OF THE INVENTION

The present invention provides in part methods and compositions for controlling infestation by Trichoplusia ni.


In one aspect, the invention provides a method for controlling infestation by a Trichoplusia ni comprising applying an effective amount of a compound of Formula Ito a site of interest whereby the infestation is controlled.







In Formula I, R1 may be methyl, ethyl, propyl, n-butyl, isopentyl (3-methylbutyl) or allyl; R2 may be at positions 2, 3 or 4 and may be H, methyl, ethyl, propyl, n-butyl, isopentyl (3-methylbutyl) or allyl; R3 may be optionally present at positions 2, 3 and 4, and is allyl; with the provisos that when R2 is at position 2, R3 if present is at position 3; when R2 is at position 3, R3 if present is at positions 2 or 4; when R2 is at position 4, R3 if present is at position 2, or when R2 is at position 4 and R3, if present, has reacted with an OH group at position 1 in a Markovnikov sense, then R3 becomes R4, a dihydrofuran.


In an alternative aspect, the invention provides a method of protecting a plant from infestation by a Trichoplusia ni comprising applying an effective amount of a compound of Formula I, to a site of interest whereby the plant is protected.


In alternative embodiments, the controlling may be one or more of oviposition deterrence, feeding deterrence, oviposition stimulation, feeding stimulation, or toxicity.


The compound of Formula I may be an oviposition deterrent, such as one or more of 3a{4,6}, 3a{3,4}, 5c{1,1-5}, 4b{4-5}, 3c{4,1-5}, 5b{6,1}, 4c {1-5}, 5b{5,1}, 3c{2,1-5}, 5c{2,1-5}, 3a{6,1-5}, 3a{6,1-5}, 3b{1,1-5}, or 3c{2,2}.


The compound of Formula I may be a feeding deterrent, such as one or more of 3c{1,1-5}, 5c{1,1}, 3c{2,2}, 5b{2, 4-5}, 3c{4,1-5}, 3b{5,1-5}, 5c{5,1-5}, 3b{4,1-5}, 3b{2,2}, 3b{1 5}, 5b{6,2-3}, 3c{2,1-5}, 5b{3,1}, 3a{4,6}, 5c{3,1}, 5b{3,2}, 5b{3,2}, 5b{6,1}, 5b{2,1}, 5b{3,2-3}, 5b{3,2}x, 3c{3,3}, 3a{3,6}, 3c{6,6}, 3b{3,3}, 3b{3,5}, 3b{6,6}, 3b{3,1-5}, 3a{4,1-5}, 3a{3,4}, 5b{5,1}, 3c{5,1-5}, 3a{3,1-5}, 3c{6,1-5}, 3c{3,6}, 3c{2,3}, 4a{1-5}, 5a{1,1-5}, 5a{2,1-5}, 6c{1-5}, 5b{3,1}, or 5b{3,1}y.


The compound of Formula I may be a oviposition stimulant, such as one or more of 3c{5,6} and 5b{2,4-5}.


The compound of Formula I may be a feeding stimulant, such as one or more of 2b{2}, 2c{1}, and 2c{3}.


The compound of Formula I may be non-toxic, or may be toxic. The toxicity may be selective for Trichoplusia ni.


In alternative embodiments, two or more compounds of Formula I may be applied simultaneously or sequentially. In alternative embodiments, a compound of Formula I may be applied in combination with another compound or treatment, such as one or more of an oviposition deterrent, an oviposition stimulant, a feeding deterrent, a feeding stimulant, an attractant, or a toxicant.


In alternative embodiments, the T. ni may be a larva or may be an adult, e.g. a female adult.


In alternative embodiments, the site of interest may be a plant or part thereof such as a cultivated plant within host range of T. ni.


In alternative embodiments, the compound of Formula I may be provided in a formulation selected from one or more of a spray, aerosol, solid, or liquid. The liquid may be an aqueous solution, oil-in-water emulsion or dispersion.


In alternative embodiments, the compound of Formula I may be provided in a controlled release form.


In an alternative aspect, the invention provides a composition including one or more compounds selected from one or more of an oviposition deterrent, an oviposition stimulant, a feeding deterrent, a feeding stimulant and toxicant.


The feeding deterrent composition may include one or more of a compound selected from 3c{1,1-5}, 5c{1,1}, 3c{2,2}, 5b{2,4-5}, 3c{4,1-5}, 3b{5,1-5}, 5c{5,1-5}, 3b{4,1-5}, 3b{2,2}, 3b{1.5}, 5b{6,2-3}, 3c{2,1-5}, 5b{3,1}, 3a{4,6}, 5c{3,1}, 5b{3,2}, 5b{3,2}, 5b{6,1}, 5b{2,1}, 5b{3,2-3}, 5b{3,2}x, 3c{3,3}, 3a{3,6}, 3c{6,6}, 3b{3,3}, 3b{3,5}, 3b{6,6}, 3b{3,1-5}, 3a{4,1-5}, 3a{3,4}, 5b{5,1}, 3c{5,1-5}, 3a{3,1-5}, 3c{6,1-5}, 3c{3,6}, 3c{2,3}, 4a{1-5}, 5a{1,1-5}, 5a{2,1-5}, 6c{1-5}, 5b{3,1}, or 5b{3,1}y.


The oviposition deterrent composition may include one or more of a compound selected from 3a{4,6}, 3a{3,4}, 5c{1,1-5}, 4b{4-5}, 3c{4,1-5}, 5b{6,1}, 4c {1-5}, 5b{5,1}, 3c{2,1-5}, 5c{2,1-5}, 3a{6,1-5}, 3a{6,1-5}, 3b{1,1-5}, or 3c{2,2}.


The oviposition stimulant composition may include one or more of a compound selected from 3c{5,6} or 5b{2,4-5}.


The toxicant composition may include one or more of a compound selected from 2b{2}, 2c{1}, 2c{3}.


This summary of the invention does not necessarily describe all features of the invention.





BRIEF DESCRIPTION OF THE DRAWINGS

These and other features of the invention will become more apparent from the following description in which reference is made to the appended drawings wherein:



FIG. 1 shows a graph with the progress of the Claisen rearrangement reaction of 3c{6,1-5} library.



FIG. 2 shows a graph with the progress of the Claisen rearrangement reaction: comparison between the ortho methoxy 3a{6,1}, meta methoxy 3b{6,1} and para methoxy 3c{6,1} library members.



FIG. 3 is a graph showing the mean weight gain of T. ni larvae exposed to cabbage plants that had been treated with compound 3c{3,6}. Three doses were tested: 1=0.0002% (2 ppm), 2=0.0005% (5 ppm), 3=0.001% (10 ppm). Data are given as a percentage of the control, larvae that were feeding on untreated plants.



FIG. 4 is a graph showing the number of larvae recovered after 6 days in a greenhouse on cabbage plants. Column 1=non-treated control; columns 2-4 plants treated with 3c{3,6} in three doses: 2=0.0002%, 3=0.0005%, 4=0.001%.





DETAILED DESCRIPTION

The present invention provides in part methods and compositions for controlling infestation by Trichoplusia ni.


The cabbage looper T. ni is an important plant pest and is a member of the moth family Noctuidae found throughout North America. The light green caterpillar (larva) grows to be about 2 inches long. The adult moth is a nocturnal brown moth. The caterpillar or larval stages generally cause extensive damage to plants. A “larva” or “larvae” as used herein refers to any caterpillar stage of T. ni. In some embodiments, a larva refers to third-instar larvae i.e., larvae that have molted twice after eclosion.


The cabbage looper is a generalist and feeds on a wide range of plants, including cultivated plants and weeds. Plants at risk for infestation by cabbage loopers, i.e., a “host plant” or a “plant within the host rage of T. ni” include without limitation cruciferous plants, such as cabbage, broccoli, cauliflower, Chinese cabbage, collards, kale, mustard, radish, rutabaga, brussel sprouts, turnip, watercress, etc. Other plants attacked by cabbage loopers include without limitation crops such as lettuce, clover, beet, pea, celery, tomatoes, rape, cantaloupe, cucumber, lima bean, parsnip, pepper, potato, snap bean, peanut, soybean, spinach, squash, sweet potato, tomato, watermelon, cotton, tobacco, etc; flowers such as chrysanthemum, hollyhock, snapdragon, sweetpea, etc.; agricultural weeds such as lambsquarters, Chenopodium album; wild lettuce, Lactuca spp.; dandelion, Taraxacum officinale; curly dock, Rumex crispus, ornamental plants; etc. Although the larvae generally damage leaves, damage to other plant parts such as watermelon rinds and flowers of various host plants has been reported. Adult moths have been reported to feed on nectar from a wide range of host flowering plants, including clover, Trifolium spp.; goldenrod, Solidago canadensis; dogbane, Apocynum spp.; sunflower, Helianthus spp.; etc. In some embodiments, the plants are plants of economic interest, such as agricultural or crop plants.


The invention provides, in part, compounds for use in controlling infestation by T. ni.


By “infestation” is meant the colonization of a site or the consumption of a plant by T. ni. In some embodiments, infestation refers to an undesirable number of T. ni, sufficient to cause damage, for example, economic damage to a plant. By “control of infestation” or “controlling infestation” is meant reduction or inhibition of infestation of a plant by T. ni by at least about 25% to at least about 100%, or any value therebetween for example about 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 99%, or 100% when compared to a control plant. In alternative embodiments, by “control of infestation” or “controlling infestation” is meant reduction or inhibition of infestation of a plant by T. ni by at least about 1-fold or more, for example, about 1.5-fold to about 100-fold, or any value therebetween for example about 2.0, 2.5, 3.0, 3.5, 4.0, 4.5, 5.0, 5.5, 6.0, 6.5, 7.0, 7.5, 8.0, 8.5, 9.0, 9.5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95-fold when compared to a control plant. Infestation may be determined using standard techniques as known in the art or described herein. For example, infestation may be measured by comparing physical features and characteristics such as leaf damage or plant growth. By “protecting a plant from infestation” is meant reducing the probability that a T. ni infestation will be established in a plant. In alternative embodiments, “control of infestation” includes oviposition deterrence, feeding deterrence, oviposition stimulation, feeding stimulation, or toxicity.


By “oviposition deterrence” is meant a decrease in egg-laying by adult female T. ni by at least about 40% to at least about 100%, or any value therebetween for example about 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 99%, or 100% when to compared to a control. Oviposition may be determined using standard techniques as known in the art or described herein.


By “feeding deterrence” is meant a decrease in feeding by T. ni larvae by at least about 50% to at least about 100%, or any value therebetween for example about 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 99%, or 100% when compared to a control. Feeding may be determined using standard techniques as known in the art or described herein.


By “oviposition stimulation” is meant an increase in egg-laying by adult female T. ni by at least about 25% to at least about 100%, or any value therebetween for example about 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 99%, or 100% when compared to a control. Oviposition may be determined using standard techniques as known in the art or described herein.


By “feeding stimulation” is meant an increase in feeding by T. ni larvae by at least about 25% to at least about 100%, or any value therebetween for example about 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 99%, or 100% when compared to a control. Feeding may be determined using standard techniques as known in the art or described herein.


By “toxicity” is meant an increase in mortality of adult or larval T. ni by at least about 25% to at least about 100%, or any value therebetween for example about 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 99%, or 100% when compared to a control. Toxicity may be determined using standard techniques as known in the art or described herein.


In alternative embodiments, the invention provides compounds for use in oviposition deterrence, feeding deterrence, oviposition stimulation, feeding stimulation, or toxicity as described herein.


Compounds for use in control of T. ni infestation include compounds according to Formula I, and mixtures thereof:







In Formula I, R1 may be hydrogen, methyl, ethyl, propyl, n-butyl, isopentyl (3-methylbutyl) or allyl; R2 may be at positions 2, 3 or 4 and may be hydrogen, methyl, ethyl, propyl, n-butyl, isopentyl (3-methylbutyl) or allyl; R3 may be optionally present at positions 2, 3 or 4, and may be allyl.


In general, in Formula I, when R2 is at position 2, R3 if present is at position 3; when R2 is at position 3, R3 if present is at positions 2 or 4; when R2 is at position 4, R3 if present is at position 2; and when R2 is at position 4 and R3, if present, has reacted with an OH group at position 1 in a Markovnikov sense, then R3 becomes R4, a dihydrofuran.


In alternative embodiments, a compound according to Formula I also includes a compound of Formula II:







In a compound of Formula II in general, R1 is at position 1, R2 is at position 2 and R3 if present is at position 3. For compounds 2a{R1} if R1 is not H, R2 is H and R3 is H; for compounds 3a {R2,R1} if R1 and R2 are not H and R3 is H; for compounds 4a{R1}, if R1 is not H, R2 is H and R3 is allyl; for compounds 5a{R2;R1} if R1 and R2 are not H and R3 is allyl. R1, R2 may be hydrogen, methyl, ethyl, propyl, n-butyl, isopentyl (3-methylbutyl) or allyl.


In alternative embodiments, a compound according to Formula I also includes a compound of Formula III:







In a compound of Formula III in general, R1 is at position 1, R2 is at position 3 and R3 if present is at position 2. For compounds 2b{R1} if R1 is not H, R2 is H and R3 is H; for 3b{R2,R2} if R1 and R2 are not H and R3 is H; for compounds 4b{R1}y if R1 is not H, R2 is H and R3 is allyl; for compounds 5b{R2,R1}y if R1 and R2 are not H and R3 is allyl. R1, R2 may be hydrogen, methyl, ethyl, propyl, n-butyl, isopentyl (3-methylbutyl) or allyl.


In alternative embodiments, a compound according to Formula I also includes a compound of Formula IV:







In a compound of Formula IV in general; R1 is at position 1; R2 is at position 3 and R3 is at position 4. For compounds 2b{R1} if R1 is not H, R2 is H and R3 is H; for compounds 3b{R2,R1} if R1 and R2 are not H and R3 is H; for compounds 4b{R1}x if R1 is not H, R2 is H and R3 is allyl; for compounds 5b{R2,R1}x if R1 and R2 and R2 are not H and R3 is allyl. R1, R2 may be hydrogen, methyl, ethyl, propyl, n-butyl, isopentyl (3-methylbutyl) or allyl.


In alternative embodiments, a compound according to Formula I also includes a compound of Formula V:







In a compound of Formula V in general R1 is at position 1, R2 is at position 4 and R3 is at position 3. For compounds 2c{R1} if R1 is not H, R2 is H and R3 is H; For compounds 3c{R2,R1} if R1 and R2 are not H and R3 is H; for compounds 4c{R1} if R1 is not H, R2 is H and R3 is allyl; for compounds 5c{R2,R1} if R1 and R2 are not H and R3 is allyl. R1, R2 may be hydrogen, methyl, ethyl, propyl, n-butyl, isopentyl (3-methylbutyl) or allyl.


In alternative embodiments, a compound according to Formula I also includes a compound of Formula VI:







In a compound of Formula VI in general R1 is at position 4; R4 is a dihydrofuran. R1 may be hydrogen, methyl, ethyl, propyl, n-butyl, isopentyl (3-methylbutyl) or allyl.


In alternative embodiments, a compound according to Formula I includes an oviposition deterrent. By “oviposition deterrent” is meant a compound according to Formula I that exhibits oviposition deterrence. In alternative embodiments, a oviposition deterrent includes a compound according to Formula I, for example, a compound of Formula I where R1 may be methyl, ethyl, propyl, butyl or isopentyl, but not H, and R2 may be methyl, ethyl, propyl, allyl, or butyl, but not H, and R3 may be H or allyl. In alternative embodiments, a oviposition deterrent includes one or more of: 3a{4,6}, 3a{3,4}, 5c{1,1-5}, 4b{4-5}, 3c{4,1-5}, 5b{6,1}, 4c {1-5}, 5b{5,1}, 3c{2,1-5}, 5c{2,1-5}, 3a{6,1-5}, 3a{6,1-5}, 3b{1,1-5}, or 3c{2,2}. In alternative embodiments, a oviposition deterrent includes one or more of: 4b{4-5}, 3c{4,1-5}, 5b{6,1}, 4c {1-5}, 5b{5,1}, 3c{2,1-5}, 5c{2,1-5}, 3a{6,1-5}, 3a{6,1-5}, or 3b{1,1-5}.


In alternative embodiments, a compound according to Formula I includes a feeding deterrent. By “feeding deterrent” is meant a compound according to Formula I that exhibits feeding deterrence. In alternative embodiments, a feeding deterrent includes a compound according to Formula I, for example, a compound of Formula I where R1 may be ethyl, propyl, allyl, butyl or isopentyl, but not H, and R2 may be methyl, ethyl, propyl, butyl, isopentyl or allyl but not H, and R3 may be allyl or H, and R4 if present may be dihydrofuran. In alternative embodiments, a feeding deterrent includes one or more of: 3c{1,1-5}, 5c{1,1}, 3c{2,2}, 5b{2,4-5}, 3c{4,1-5}, 3b{5,1-5}, 5c{5,1-5}, 3b{4,1-5}, 3b{2,2}, 3b{1.5}, 5b{6,2-3}, 3c{2,1-5}, 5b{3,1}, 3a{4,6}, 5c{3,1}, 5b{3,2}, 5b{3,2}, 5b{6,1}, 5b{2,1}, 5b{3,2-3}, 5b{3,2}x, 3c{3,3}, 3a{3,6}, 3c{6,6}, 3b{3,3}, 3b{3,5}, 3b{6,6}, 3b{3,1-5}, 3a{4,1-5}, 3a{3,4}, 5b{5,1}, 3c{5,1-5}, 3a{3, 1-5}, 3c{6,1-5}, 3c{3,6}, 3c{2,3}, 4a{1-5}, 5a{1,1-5}, 5a{2,1-5}, 6c{1-5}, 5b{3,1}, or 5b{3,1}y.


In alternative embodiments, a feeding deterrent includes a compound according to Formula I that exhibits greater than about 80% feeding deterrence and a DC50 of less than about 20 μg/cm2. In alternative embodiments, a feeding deterrent includes one or more of: 3c{4,1-5}, 3b{4,1-5}, 5b{6,2-3}, 3c{2,1-5}, 3a{4,6}, 5c{3,1}, 5b{3,2}, 5b{3,2}, 5b{6,1}, 5b{3,2-3}, 3c{3,3}, 3a{3,6}, 3c{6,6}, 3b{3,3}, 3b{3,5}, 3b{6,6}, 3a{4,1-5}, 5b{5,1}, 3c{5,1-5}, 3a{3,1-5}, 3c{6,1-5}, 3c{3,6}, 5a{1,1-5}, 5a{2,1-5}, 6c{1-5}, 5b{3,1}, or 5b{3,1}y.


In alternative embodiments, a feeding deterrent includes a compound having a Feeding Deterrence Index (Feeding Deterrence/(DC50×mortality) of at least about 4, 6, or 9. In alternative embodiments, a feeding deterrent includes a compound having a Feeding Deterrence Index of at least about 35, 40, 45, 50, 55, 60, 65, 70, 75, 70, 85, 90, 95 or 100. In alternative embodiments, a feeding deterrent includes a compound having a Feeding Deterrence Index of at least about 200, 500, 1000, 1500, or 2000.


In alternative embodiments, a compound according to Formula I includes an oviposition stimulant. By “oviposition stimulant” is meant a compound according to Formula I that exhibits oviposition stimulation. In alternative embodiments, a oviposition stimulant includes a compound according to Formula I, for example, a compound of Formula I where R1 may be allyl, butyl or isopentyl and R2 may be ethyl or isopentyl and R3 may be allyl or H. In alternative embodiments, a oviposition stimulant includes one or both of: 3c{5,6} or 5b{2,4-5}.


In alternative embodiments, a compound according to Formula I includes a feeding stimulant. By “feeding stimulant” is meant a compound according to Formula I that exhibits feeding stimulation. In alternative embodiments, a feeding stimulant includes a compound according to Formula I, for example, a compound of Formula I where R1 may be methyl, ethyl or propyl, and R2 may be H. In alternative embodiments, a feeding stimulant includes one or more of: 2b{2}, 2c{1}, or 2c{3}.


In alternative embodiments, a compound according to Formula I includes a toxicant. By “toxicant” is meant a compound according to Formula I that exhibits toxicity. In alternative embodiments, a toxicant includes a compound according to Formula I, for example, a compound of Formula I where R1 may be ethyl and —OR2 is para to OR1 (=at position 4 relative to OR1) and R2 may be methyl, ethyl, propyl, butyl or isopentyl. In alternative embodiments, a toxicant includes one or more of: 5b{6,1}, 5a{2,1-5}, 5c{5,1-5}, 3b{4,1-5}, 3b{1,5}, 3b{1,6}, 5b{6,2-3}, 5b{6,4-5}, 3b{2,2}, 5c{1,1}, 3a{5,5}, 3b{6,4-5}, 5a{3,1-5}, 3b{3,1-5}, 4b{4-5}, 5b{1,1}, 3c{3,1-5}, 3c{4,1-5}, 3b{6,2-3}, 5b{1,2-3}, 4b{2-3}, 3b{3,3}, 3c{2,1-5}, or 3c{2,2}.


In alternative embodiments, a compound according to Formula I is non-toxic. By “non-toxic” is meant a mortality rate of adult or larval T. ni of less than about 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 99%, or 100% when compared to a control. Toxicity may be determined using standard techniques as known in the art or described herein. In alternative embodiments, a non-toxic compound includes a compound according to Formula I, for example, a compound of Formula I where R1 may be methyl, ethyl or allyl and R2 may be propyl or allyl. In alternative embodiments, a non-toxic compound includes a compound according to Formula I. In alternative embodiments, a non-toxic compound includes one or more of: 3c{3,6}, 5b{3,1}y, 5b{6,1}, 5b{3,2}x, 5b{3,2}, 5b{3,2}, 6c{1-5}, 5b{5,1}, 5c{3,1}, 3c{6,1-5}, 5b{3,1}, 5b{2,1}, 5b{3,2-3}, 3a{4,1-5}, 3b{3,5}, 5b{2,4-5}, or 5b{3,1}.


In alternative embodiments, a compound according to the invention is selective. By “selective” is meant that a compound exhibits an activity such as one or more of oviposition deterrence, feeding deterrence, oviposition stimulation, feeding stimulation, or toxicity towards T. ni but not other pests, such as other noctuid moths or insects, or other organisms. In some embodiments, by “selective” is meant that a compound exhibits an activity such as one or more of oviposition deterrence, feeding deterrence, oviposition stimulation, feeding stimulation, or toxicity towards larval T. ni but not adults, and vice versa.


In alternative embodiments, a compound according to the invention, as used herein, may include one or more than one compound as described in Formula I, or in the Tables and Figures herein. Accordingly, in some embodiments, sets or mixtures of the compounds as described in Formula I, or in the Tables and Figures herein are included in the meaning of the term “compound”. In alternative embodiments, one or more than one compound as described in Formula I, or in the Tables and Figures herein, may be specifically excluded from the methods or compositions according to the invention.


A compound according to the invention may be applied to a site of interest to control infestation by T. ni. By “site of interest” is meant any area or region that is infested with, or at risk of infestation by, T. ni or is in the vicinity of such an area or region. Sites of interest include without limitation a plant, an area that contains a plant, an area that is intended to contain a plant, an area that is in the vicinity of a plant, etc. Accordingly, a site of interest may be a host plant, field (e.g., a vegetable field), greenhouse, habitat, garden, bait, lure, trap, film, etc. In alternative embodiments, a site of interest may be an area or region planted with alternative host plants, so that the T. ni may be lured to the alternative host plants.


By “applied” or “applying” is meant contacting a T. ni with an effective amount of a compound. In alternative embodiments, by “applied” or “applying” is meant placing an effective amount of a compound on, in, or in the vicinity of a site of interest, as appropriate. The application method may take any form such as spraying, fogging, dusting, sprinkling, aerosolizing, e.g., of a field or greenhouse, or targeted applications such as direct application to a host plant or part thereof, or placement in a bait or trap, etc.


By “effective amount” is meant an amount or concentration of a compound that is sufficient to modulate the number of T. ni in a site of interest by at least about 25% to at least about 100%, or any value therebetween for example about 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 99%, or 100% when compared to a similar site in the absence of the compound. In alternative embodiments, by “effective amount” is meant an amount or concentration of a compound that is sufficient to modulate the number of T. ni in a site of interest by at least about 1-fold or more, for example, about 1.5-fold to about 100-fold, or any value therebetween for example about 2.0, 2.5, 3.0, 3.5, 4.0, 4.5, 5.0, 5.5, 6.0, 6.5, 7.0, 7.5, 8.0, 8.5, 9.0, 9.5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95-fold when compared to a similar site in the absence of the compound. By “modulate,” “modulation” or “modulating” is meant changing, by either increase or decrease. Accordingly, for a compound having for example oviposition deterrent, feeding deterrent, or toxicant activity, the appropriate modulation would be to decrease the number of T. ni in a site of interest (such as a field or greenhouse or also, for a toxicant, bait or trap). Conversely, for a compound having for example oviposition stimulation or feeding stimulation activity, the appropriate modulation would be to increase the number of T. ni in a site of interest (such as a bait or trap). It is to be understood that the effective amount of a compound will vary, depending on such factors as contemplated use, life stage of T. ni, population density, site of interest, release rate, time of year, host crop, ambient moisture, temperature, etc.


In alternative embodiments, two or more compounds according to the invention may be applied to control infestation by T. ni.


In alternative embodiments, a compound according to the invention may be applied in combination with one or more other compounds, treatments, or systems to control infestation by T. ni. For example, feeding stimulants such as fructose, fucose, glucose, or sucrose; feed such as molasses; toxicants such as insecticides, fungicides, nematocides, bactericides, acaricides; attractants such as pheromones; growth regulators such as rooting stimulants; repellents, etc. may be combined with a compound according to the invention.


The application may be simultaneous or sequential. For example, an oviposition or feeding stimulant as described herein may be combined with a toxicant, such as an insecticide, in a “lure and kill” or “attract and kill” treatment. In other embodiments, a toxicant as described herein may be combined with a feeding stimulant, feed, or attractant. Alternatively, a feeding deterrent may be applied to target larvae and an oviposition deterrent may be applied to target female adults at different times. Similarly, a feeding deterrent may be applied to target larvae and an oviposition stimulant may be applied to target female adults at different times. The application may be varied to, for example, minimize the build up of resistance to a particular treatment or compound.


The compounds or compositions according to the invention may be substantially pure compounds or mixtures thereof or may be formulated with a suitable additive as appropriate depending on the contemplated end use. For example, a compound or composition may be formulated with suitable additives such as carriers, diluents, emulsifiers, antioxidants, thickeners, fillers, preservatives, surfactants, etc., including without limitation crop spray oils, or any other suitable additive. It is to be understood that any suitable formulation may be used, depending on the contemplated end use. For example, the formulations may be generally non-toxic, except for those containing a toxicant or insecticide where high mortality is a desired outcome.


In some embodiments, the compounds or compositions may be formulated in controlled release forms. The formulations may be solid, such as granules, dusts, or pellets, such as granules for direct use (i.e., without admixture with a liquid), water-dispersible granules; powders, wettable powders, dry (soluble) powders; etc. or may be liquid, such as an aqueous solution, flowable formulation, an emulsion e.g., oil-in-water emulsion, a suspension, a dispersion, etc. In some embodiments, the compounds may be formulated with a co-solvent, such as isopropanol. The compounds may be formulated for direct use (i.e., “ready-to-use” formulation) or as a concentrate.


In some embodiments, the compounds or compositions may be provided in any appropriate trap, dispenser or device known in the art.


The compounds or compositions may be used to control infestation by T. ni. In alternative embodiments, selected compounds or compositions may be used to deter or stimulate larval feeding or to deter or stimulate adult female oviposition. Accordingly, in alternative embodiments, the compounds or compositions may be used to influence host plant selection by T. ni.


Kits


The invention provides kits for use in control of T. ni infestation. In one embodiment, the kit includes a composition containing an effective amount of a compound according to the invention for application to a site of interest. In alternative embodiments, the kit may include a container containing another compound or treatment such as a toxicant such as an insecticide, attractant, etc.; the container may be any suitable container depending on the contemplated end use. The compound according to the invention may be provided together with instructions for administration to a site of interest. The instructions may include directions for use and may be provided as part of the kit or separately.


EXAMPLES

The following examples are intended to illustrate embodiments of the invention and should not be construed as limiting.


Example 1
Synthesis of Dialkoxybenzene Test Compounds

Synthesis Scheme. Dialkoxybenzene minilibraries (consisting of four to five compounds) and pure compounds were synthesized. Briefly, dialkoxybenzenes were synthesized from the corresponding dihydroxybenzenes (1 (a-c)) by monoalkylation (Scheme 1). The pure monoalkylated compounds were mixed in equimolar amounts, for the synthesis of minilibraries, and subjected to a second round of alkylation. Thus, the minilibraries include compounds with one alkyl group constant and the other one variable.









Scheme 1





General approach for the synthesis of mini-libraries

















Reaction conditions:


1) base (NaH, K2CO3or Cs2CO3), solvent (DMF or acetone), alkyl halide (MeI, EtI, PrI, BuBr, iPentBr or AllylBr), room temperature or reflux;


2) for 3(a-c) {6,1-5} neat, 180° C., 10 h (Scheme 2);


(3) K2CO3, alkyl halide, acetone, reflux;


4) for 3c{6,1-5} neat, 180° C., 30 h (Scheme 2).



The meta product from the Claisen Rearrangement (Set B) results in two products and will be identified as: 4bx{n} for 5-alkoxy-2-allyl phenol and 4by{n} for 3-alkoxy-2-allyl phenol (see Scheme 2) (similarly for their alkylated derivatives).







An alternate depiction of Scheme 1 is described in more detail below (Scheme 1-1):










All solvents used were of analytical grade. Resorcinol monoacetate was from Aldrich. Compounds 2c{1}, 2c{2} and 2c{3} were synthesized and also purchased from Aldrich. Commercial grade solvents were distilled under nitrogen prior to use with the exceptions as follow: dried THF was obtained from a MBRAUN LTS 350 solvent purification system and HPLC grade acetone was used without further treatment. Reagents were used without further purification. The 1H and 13C NMR spectra were recorded in CDCl3 on Bruker 400 or 600 MHz spectrometers or a Varian 500 MHz spectrometer.


Gas chromatography (GC) was done on Hewlett Packard 5890 using a SPB-5 column Supelco, 30 m, 0.25 mm i.d, (0.25 μm film), programmed at 100° C. (5 min), 10° C./min, and 200° C. (0 min), 50° C./min, 250° C. (4-14 min). The gas chromatographic data are reported as retention indices (RI). MS: GC-mass spectra were recorded on a Varian Saturn 2000 MS coupled to a CP 300 GC, equipped with a SPB-5 GC column (same type as above), programmed as above. Mass spectra were acquired in EI mode [2 μscans (0.55 s/scan), emission current (30 μamp), scanning single ion storage SIS (49-375 m/z)]. HRMS was recorded on a 6210 Series Time-of-Flight LC/MS System.


The identity of the members in each library was confirmed by 1H NMR and GC-MS techniques.


Optimization of the mono alkylation of dihydroxybenzenes 1(a-c) revealed that direct alkylation resulted in high yields. Ortho (a), meta (b) or para (c) substituted dihydroxy benzene 1(a-c) was deprotonated and reacted with an alkyl halide to afford mono 2(a-c){n} and dialkoxy 3(a-c){n,n} products (Scheme 1 or 1-1). Tuning of the experimental conditions (base, solvent and reaction time, see Methods A-E) allowed the preferential synthesis of either monoalkylated or dialkylated products. Mono- and dialkylated products were separated using their acid/base properties. The monoalkoxy compounds 2(a-c){n} were used for the synthesis of libraries, and the dialkoxy compounds 3(a-c){n,n} with identical alkyl groups were used for characterization and biological testing (Table 1).









TABLE 1







Purity of Dialkoxy Compounds 3(a-c){n,n} Synthesized


for Characterization and Biological Evaluation









no.
Compound
Puritya












1
3a{1,1}
94


2
3a{2,2}
100


3
3a{3,3}
100


4
3a{4,4}
100


5
3a{5,5}
100


6
3a{6,6}
99


7
3b{1,1}
94


8
3b{2,2}
98


9
3b{3,3}
98


10
3b{4,4}
100


11
3b{5,5}
100


12
3b{6,6}
95


13
3c{1,1}
95


14
3c{2,2}
95


15
3c{3,3}
96


16
3c{4,4}
99


17
3c{5,5}
98


18
3c{6,6}
99






aPurity was determined by GC.







Synthesis of Alkoxy Phenols and Dialkoxy Benzenes


Method A: Anhydrous K2CO3 (5 eq) was added to a solution of acetoxy-alkoxy benzene (1 eq) in CH3OH (25 mL) and the mixture was stirred at room temperature and monitored by TLC (hexanes-EtOAc, 8:2). When reaction was complete, it was concentrated under reduced pressure. The residue was then diluted with CHCl3 (25 mL) and water (25 mL), the organic layer was dried over anhydrous Na2SO4 and concentrated under reduced pressure. In certain cases the crude product was purified by flash column chromatography (hexanes: EtOAc, 7:3) to afford pure alkoxy phenol.


Method B: The dihydroxybenzene (hydroquinone, resorcinol or catechol, 1 eq) was added to a suspension of anhydrous K2CO3 (10 eq) in CH3OH (30 mL). The mixture was stirred at room temperature for 1 h then the alkylating reagent (10 eq) was added and reaction was monitored by TLC (hexanes-EtOAc, 7:3). When reaction was complete, the mixture was concentrated under reduced pressure and diluted with CHCl3 (30 mL) and water (30 mL). The organic layer was separated, dried over anhydrous Na2SO4 and concentrated under reduced pressure to afford a crude solid which was purified by flash column chromatography (hexane-EtOAc, 7:3) to yield pure products.


Method C: The dihydroxybenzene (hydroquinone, resorcinol or catechol, 1 eq) was added to a suspension of Cs2CO3 (0.5 eq) in DMF (5 mL) and the mixture was stirred at room temperature for 2 h. The alkylating reagent (1 eq) was then added and the reaction mixture was heated at reflux and monitored by TLC (hexanes-EtOAc, 25:1). When reaction was complete (usually after 20 h), HCl (1%, 20 mL) was added and the mixture was extracted with CHCl3 (3×30 mL). The combined organic layers were washed with water (3×30 mL) and brine (30 mL), dried over anhydrous Na2SO4 and concentrated under reduced pressure. The crude obtained was purified by flash chromatography (hexanes-EtOAc, 25:1) to yield pure products.


Method D: The dihydroxybenzene (hydroquinone, resorcinol or catechol, 1 eq) was added to a suspension of K2CO3 (1 eq) in acetone (20 mL) and the mixture was stirred at room temperature for 2 h. The alkylating reagent (1.2 eq) was then added and the reaction mixture was heated at reflux and monitored by TLC (CHCl3). When reaction was complete, the mixture was filtered and the filtrate was concentrated under reduced pressure. The residue was diluted with C6H6 (30 mL) and washed with aqueous NaOH (10%, 40 mL). The organic layer was dried over anhydrous Na2SO4 and concentrated under reduced pressure to afford the corresponding pure dialkoxy benzene product. The basic aqueous layer was cooled in an ice bath and acidified with concentrated HCl. The solid alkoxy phenol was collected from this mixture by vacuum filtration.


Method E: The dihydroxybenzene (hydroquinone, resorcinol or catechol, 1 eq) was added to a suspension of NaH (5 eq) in DMF (3 mL). The alkylating reagent (5 eq) was then added and the reaction mixture was stirred at room temperature and monitored by TLC. When reaction was complete, a solution of saturated NH4Cl (10 mL) was slowly added and the aqueous phase was extracted with CHCl3 (2×15 mL). The combined organic layers were washed with water (10×15 mL), dried over anhydrous Na2SO4 and concentrated under reduced pressure. The crude oil was purified by flash column chromatography using hexanes:EtOAc as solvents to afford the corresponding pure compounds.


2-Ethoxy phenol 2(a){2} (Method C, 28%, Method D, 70%): GC (RI 1157, 96.7%); 1H NMR δ: 1.46 (t, J=7.0 Hz, 3H, CH3), 4.12 (q, J=7.0 Hz, 2H, OCH2), 5.76 (broad s, 1H, OH), 6.83-6.90 (m, 3H, ArH), 6.94-6.97 (m, 1H, ArH); 13C NMR δ: 14.8, 64.3, 111.6, 114.4, 120.0, 121.3, 145.7, 145.8; MS m/z (relative intensity): 139 (M++H, 41%), 138 (M+, 100%); IR (cm−1): 3535 (broad), 3054, 2984, 1611, 1596, 1502, 1040, 925, 743.


2-Propoxy phenol 2a{3} (Method C, 26%, Method D, 80%): GC (RI 1251, 100%); 1H NMR δ: 0.94 (t, J=7.4 Hz, 3H, CH3), 1.70-1.77 (m, 2H, CH2), 3.89 (q, J=6.5 Hz, 2H, OCH2), 5.64 (broad s, 1H, OH), 6.70-6.86 (m, 4H, ArH); 13C NMR δ: 10.4, 22.5, 70.3, 111.6, 114.4, 120.1, 121.2, 145.8, 145.9; MS m/z (relative intensity): 153 (M++H, 19%), 152 (M+, 100%); IR (cm−1): 3540 (broad), 3054, 2968, 2878, 1612, 1596, 1503, 1260, 978, 743.


2-Butoxy phenol 2a{4} (Method C, 51%): GC (RI 1353, 98.7%); 1H NMR δ: 1.00 (t, J=7.4 Hz, 3H CH3), 1.47-1.55 (m, 2H, CH2), 1.78-1.84 (m, 2H, CH2), 4.05 (t, J=6.5 Hz, 2H, OCH2), 5.69 (broad s, 1H, OH), 6.82-6.88 (m, 3H, ArH), 6.92-6.95 (m, 1H, ArH); 13C NMR δ: 13.8, 19.2, 31.2, 68.5, 111.5, 114.4, 120.0, 121.2, 145.8; MS m/z (relative intensity): 165 (M++H, 20%), 166 (M+, 100%); IR (cm−1): 3542 (broad), 3054, 2962, 2872, 1612, 1597, 1503, 1261, 1106, 783, 741.


2-(3-Methyl-butyloxy)phenol 2a{5} (Method C, 52%): GC (RI 1412, 99.9%); 1H NMR δ: 0.99 (d, J=6.6 Hz, 6H, CH3), 1.73 (apparent q, J=6.8 Hz, 2H, CH2), 1.81-1.89 (m, 1H, CH), 4.08 (t, J=6.6 Hz, 2H, OCH2), 5.70 (broad s, 1H, OH), 6.82-6.90 (m, 3H, ArH), 6.95-6.96 (m, 1H, ArH); 13C NMR δ: 22.5, 25.1, 37.9, 67.2, 111.5, 114.4, 120.0, 121.2, 145.7, 145.9; MS m/z (relative intensity): 181 (M++H, 19%), 180 (M+, 100%); IR (cm−1): 3544 (broad), 3054, 2872, 1611, 1597, 1503, 1260, 742.


2-Allyloxy phenol 2a{6} (Method D, 54%): GC (RI 1240, 100%); 1H NMR δ: 4.61 (dt, J=5.5, 1.4 Hz, 2H, OCH2), 5.32 (dq, 10.5, 1.3 Hz, 1H, CH2), 5.41 (dq, J=17.3, 1.5 Hz, 1H, CH2), 5.66 (s, 1H, OH), 6.03-6.11 (m, 1H, CH), 6.81-6.95 (m, 4H, ArH); 13C NMR δ: 69.8, 112.2, 114.7, 118.3, 120.0, 121.7, 132.8, 145.5, 145.9; MS m/z (relative intensity): 151 (M++H, 25%), 150 (M+, 100%); IR (cm−1): 3526 (broad), 2870, 1597, 1503, 1465, 1107, 791, 746.


3-Methoxy phenol 2b{1} (Method A, 50%): GC (RI 1219, 100%); 1H NMR δ: 3.7 (s, 3H, CH3), 5.38 (s, 1H, OH), 6.46-6.49 (m, 2H, ArH), 6.52-6.54 (m, 1H, ArH), 7.14 (t, J=8.1 Hz, 1H, ArH); 13C NMR δ: 55.2, 101.5, 106.4, 108.0, 130.2, 156.6, 160.6; IR (cm−1): 3397 (broad), 1598, 1286, 1148, 1041, 765.


3-Ethoxy phenol 2b{2} (Method A, 50%): GC (RI 1311, 96.7%); 1NMR δ: 1.39 (t, J=7.0 Hz, 3H, CH3), 3.99 (q, J=7.0 Hz, 2H, OCH2), 6.26 (broad s, 1H, OH), 6.45-6.48 (m, 2H, ArH), 6.50-6.53 (m, 1H, ArH), 7.13 (t, J=8.0 Hz, ArH); 13C NMR δ: 14.6, 63.6, 102.1, 107.1, 107.9, 130.1, 156.6, 160.0; IR (cm−1): 3449 (broad), 2981, 1596, 976, 765.


3-Propoxy phenol 2b{3} (Method A, 47%): GC (RI 1404, 100%); 1H NMR δ: 1.03 (t, J=7.4 Hz, 3H, CH3), 1.76-1.83 (m, 2H, CH2), 3.89 (q, J=6.7 Hz, 2H, OCH2), 5.67 (broad s, 1H, OH), 6.43-6.45 (m, 2H, ArH), 6.50-6.53 (m, 1H, ArH), 7.11-7.14 (m, 1H, ArH); 13C NMR δ: 10.4, 22.4, 69.6, 102.1, 107.1, 107.7, 130.1, 156.5, 160.3; IR (cm−1): 3415 (broad), 2966, 2878, 1596, 1493, 1146, 1004, 766.


3-(3-Methyl-butyloxy)phenol 2b{4} (Method B, 9%): GC (RI 1556, 99.9%); 1H NMR δ: 0.96 (d, J=6.7 Hz, 6H, CH3), 1.67 (apparent q, J=6.7 Hz, 2H, CH2), 1.78-1.86 (m, 1H, CH), 3.96 (t, J=6.7 Hz, 2H, OCH2), 5.46 (broad s, 1H, OH), 6.42-6.44 (m, 2H, ArH), 6.50-6.52 (m, 1H, ArH), 7.10-7.15 (m, 1H, ArH); 13C NMR δ: 22.53, 25.00, 37.9, 66.5, 102.1, 107.1, 107.6, 130.1, 156.6, 160.4; IR (cm−1): 3419 (broad), 2955, 2870, 1599, 1467, 1142, 839, 764.


4-Methoxy phenol (Method A, 77%) 2c{1}: GC (RI 1170, 98.0%); 1H NMR δ: 3.76 (s, 3H, CH3), 5.53 (s, 2H, OH), 6.76-6.80 (m, 4H, ArH); 13C NMR δ: 55.8, 114.9, 116.1, 149.5, 153.5; MS m/z (relative intensity): 125 (M++H, 31%), 124 (M+, 100%), 109 (80), 81 (54).


4-Ethoxy phenol 2c{2}: GC (RI 1248, 98.5%); 1H NMR δ: 1.39 (t, J=7.0 Hz, 3H, CH3), 3.99 (q, J=7.0 Hz, 2H, OCH2), 5.91 (s, 1H, OH), 6.75-6.80 (m, 4H, ArH); 13C NMR δ: 14.8, 64.3, 115.8, 116.1, 149.5, 152.7.


4-Propoxy phenol 2c{3}: GC (RI 1325, 95.0%); 1H NMR δ: 1.01 (t, J=7.4 Hz, 3H, CH3), 1.74-1.81 (m, 2H, CH2), 3.86 (q, J=6.5 Hz, 2H, OCH2), 1.68 (broad s, 1H, OH), 6.74-6.80 (m, 4H, ArH); 13C NMR δ: 10.1, 22.3, 70.2, 115.5, 115.9, 149.6, 152.5; IR (cm−1): 3397 (broad), 2873, 1511, 1455, 1237, 982, 823, 793.


4-Butoxy phenol 2c{4} (Method C, 42%, Method D, 40%): GC (RI 1483, 99.9%); 1H NMR δ: 0.96 (t, J=7.3 Hz, 3H, CH3), 1.44-1.52 (m, 2H, CH2), 1.71-1.77 (m, 2H, CH2), 3.91 (t, J=6.5 Hz, 2H, OCH2), 4.79 (broad s, 1H, OH), 6.74-6.80 (m, 4H, ArH); 13C NMR δ: 13.8, 19.2, 68.5, 115.7, 116.0, 149.3, 153.1; MS m/z (relative intensity): 167 (M++H, 43%), 166 (M+, 100%); IR (cm−1): 3403 (broad), 2957, 2871, 1514, 1374, 1242, 971, 822, 768.


4-(3-Methyl-butyloxy)phenol 2c{5} (Method B, 24%, Method C, 29%; Method D, 32%): GC (RI 1552, 100%); 1H NMR δ: 0.96 (d, J=6.7 Hz, 5H, CH3), 1.65 (apparent q, J=6.8 Hz, 2H, CH2), 1.78-1.86 (m, 1H, CH), 3.92 (t, J=6.6 Hz, 2H, OCH2), 4.67 (broad s, 1H, OH), 6.74-6.80 (m, 4H, ArH); 13C NMR δ: 22.6, 25.0, 38.1, 67.1, 115.6, 116.0, 149.3, 153.3; MS m/z (relative intensity): 181 (M++H, 18%), 180 (M+, 100%), 110 (95%); IR (cm−1): 3404 (broad), 2959, 2866, 1622, 1426, 1386, 1236, 820, 749.


4-Allyloxy phenol 2c{6} (Method D, 18%): GC (RI 1372, 100%); 1H NMR δ: 4.48 (d, J=5.4 Hz, 2H), 5.28 (dd, J=10.5, 1.1 Hz, 1H), 5.40 (dd, J=17.3, 1.6 Hz, 1H), 5.45 (broad s, 1H, OH), 6.01-6.09 (m, 1H), 6.75-6.77 (m, 2H, ArH), 6.80-6.82 (m, 2H, ArH); 13C NMR δ: 69.7, 115.9, 116.0, 117.7, 133.4, 149.5, 152.5; MS m/z (relative intensity): 151 (M++H, 55%), 150 (M+, 100%).


1,2-Dimethoxy benzene 3a{1,1} (Method E from 2c-1 as starting material, 72%): GC (RI 1152, 99%); 1H NMR δ: 3.88 (s, 6H, CH3), 6.87-6.94 (m, 4H, ArH); 13C NMR δ: 55.6, 111.2, 120.7, 148.9; MS m/z (relative intensity): 139 (M++H, 11%), 138 (M+, 100%), 123 (50%), 95 (56%), 77 (69%); IR (cm−1): 3065, 2936, 2835, 1593, 1254, 1123, 1028, 746.


1,2-Diethoxy benzene 3a{2,2} (Method D, 24%): GC (RI 1240, 100%); 1H NMR δ: 1.46 (t, J=7.0 Hz, 6H, CH3), 4.10 (q, J=7.0 Hz, 4H, CH2), 6.90 (s, 4H, ArH); 13C NMR δ: 14.8, 64.4, 113.5, 120.9, 128.2, 148.7; MS m/z (relative intensity): 167 (M++100%), 166 (M+, 96%); IR (cm−1): 3063, 2987, 2871, 1592, 1506, 1392, 1034, 930, 738.


1,2-Dipropoxy benzene 3a{3,3} (Method D, 18%): GC (RI 1420, 100%); 1H NMR δ: 0.94 (t, J=7.4 Hz, 6H, CH3), 1.70-1.78 (m, 4H, CH2), 3.86 (q, J=6.6 Hz, 4H, CH2), 6.74-6.82 (m, 4H, ArH); 13C NMR δ: 10.5, 22.6, 70.7, 114.1, 121.0, 149.2; MS m/z (relative intensity): 195 (M++H, 100%), 194 (M+, 84%); IR (cm−1): 3064, 2963, 2876, 1593, 1503, 1255, 1125, 981, 739.


1,2-Dibutoxy benzene 3a{4,4} (Method E from 2a{4} as starting material, 30%): GC (RI 1603, 100%); 1H NMR δ: 0.98 (t, J=7.4 Hz, 6H, CH3), 1.47-1.56 (m, 4H, CH2), 1.77-1.83 (m, 4H, CH2), 4.00 (t, J=6.6 Hz, 4H, OCH2), 6.87-6.91 (m, 4H, ArH); 13C NMR δ: 13.9, 19.2, 31.4, 68.9, 114.0, 120.9, 149.2; MS m/z (relative intensity): 223 (M++H, 6%), 222 (M+, 41%); 110 (100%); IR (cm−1): 2958, 2872, 1593, 1502, 1253, 1221, 737.


1,2-Di-(3-methyl-butyloxy)benzene 3a{5,5} (Method E from 2c-i5 as starting material, 53%): GC (RI 1708, 100%); 1H NMR δ: 0.96 (d, J=6.7 Hz, 12H, CH3), 1.71 (apparent q, J=6.8 Hz, 4H, CH2), 1.82-1.90 (m, 2H, CH), 4.02 (t, J=6.7 Hz, 4H, OCH2), 6.87-6.91 (m, 4H, ArH); 13C NMR δ: 22.6, 25.1, 38.0, 67.6, 114.0, 120.9, 149.2; MS m/z (relative intensity): 251 (M++H, 10%), 250 (M+, 53%); 180 (21%); 110 (100%); IR (cm−1): 3064, 2953, 2870, 1593, 1506, 1385, 1055, 982, 739.


1,2-Diallyloxy benzene 3a{6,6} (Method D, 24%): GC (RI 1411, 100%); 1H NMR δ: 4.62 (dt, J=5.3, 1.5 Hz, 4H, OCH2), 5.27-5.30 (m, 2H, CH2), 5.41-5.45 (m, 2H, CH2), 6.06-6.14 (m, 2H, CH), 6.89-6.94 (m, 4H, Art); 13C NMR δ: 69.8, 114.2, 117.4, 121.2, 133.5, 148.5; MS m/z (relative intensity): 191 (M++H, 62%), 190 (M+, 100%); IR (cm−1): 0.3081, 2858, 1648, 1591, 1507, 1124, 921, 740.


1,3-Dimethoxy benzene 3b{1,1} (Method E, 76%): GC (RI 1181, 94.0%); 1H NMR δ: 3.81 (s, 6H, CH3), 6.51-6.56 (m, 3H, ArH), 7.16 (t, J=8.2 Hz, 1H, ArH); 13C NMR δ55.1, 100.4, 106.1, 129.8, 160.8; IR (cm−1): 3001, 2957, 2835, 1593, 1337, 1152, 1050, 763.


1,3-Diethoxy benzene 3b{2,2} (Method E): GC (RI 1321, 98.3%); 1H NMR δ: 1.42 (t, J=7.0 Hz, 6H, CH3), 4.02 (q, J=7.0 Hz, 4H, CH2), 6.47-6.51 (m, 3H, ArH), 7.16 (t, J=8.2 Hz, 1H, ArH); 13C NMR δ: 14.8, 63.3, 101.3, 106.6, 129.7, 160.1; IR (cm−1): 2980, 1603, 1493, 1475, 1150, 1048.


1,3-Dipropoxy benzene 3b{3,3} (Method E, 65%): GC (RI 1504, 98%); 1H NMR δ: 1.04 (t, J=7.4 Hz, 6H, CH3), 1.77-1.85 (m, 4H, CH2), 3.91 (q, J=6.5 Hz, 4H, CH2), 6.48-6.51 (m, 3H, ArH), 7.16 (t, J=8.2 Hz, 1H, ArH); 13C NMR δ: 10.5, 22.6, 69.4, 101.4, 106.6, 129.7, 160.3; IR (cm−1): 2964, 2877, 1601, 1492, 1470, 1287, 1263, 759.


1,3-Dibutoxy benzene 3b{4,4} (Method B, 16%): GC (RI 1701, 100%); 1H NMR δ: 0.97 (t, J=7.4 Hz, 6H, CH3), 1.44-1.52 (m, 4H, CH2), 1.73-1.78 (m, 4H, CH2) 3.94 (t, J=6.5 Hz, 4H, CH2), 6.46-6.49 (m, 3H, ArH), 7.15 (t, J=8.1 Hz, 1H, Art); 13C NMR δ: 13.9, 19.2, 31.3, 67.6, 101.4, 106.6, 129.7, 160.3; MS m/z (relative intensity): 223 (M++H, 36%), 222 (M+, 100%).


1,3-Di-(3-methyl-butyloxy)benzene 3b{5,5} (Method E, 63%): GC (RI 1826, 100%); 1H NMR δ: 0.97 (d, J=6.5 Hz, 12H, CH3), 1.68 (apparent q, J=6.8 Hz, 4H, CH2), 1.80-1.88 (m, 2H, CH), 3.98 (t, J=6.7 Hz, 4H, OCH2), 6.47-6.61 (m, 3H, ArH), 7.16 (t, J=8.2 Hz, 1H, ArH); 13C NMR δ: 22.6, 25.0, 38.0, 66.3, 101.4, 106.6, 129.7, 160.4; MS m/z (relative intensity): 251 (M++H, 48%), 250 (M+, 100%); IR (cm−1): 2948, 2866, 1580, 1471, 1288, 1158, 850, 762, 689.


1,3-Diallyloxy benzene 3b{6,6} (Method B, 41%): GC (RI 1486, 95%); 1H NMR δ: 4.52 (dt, J=1.5 and 5.3 Hz, 4H, OCH2), 5.29 (dq, J=1.3 and 10.5 Hz, 2H, CH═CH2), 5.42 (dq, J=1.6 and 17.3 Hz, 2H, CH═CH2), 6.06 (ddt, J=5.3, 10.6 and 17.2 Hz, 2H, CH), 6.51-6.54 (m, 3H, ArH), 7.17 (t, J=8.0 Hz, 1H, ArH); 13C NMR δ: 68.8, 101.9, 107.1, 117.7, 129.8, 133.2, 159.7; MS m/z (relative intensity): 191 (M++H, 70%), 190 (M+, 100%).


1,4-Dimethoxy benzene 3c{1,1} (Method B, 65%): GC (RI 1115, 95.0%); 1H NMR δ: 3.77 (s, 6H, CH3), 6.84 (s, 4H, ArH); 13C NMR δ 55.7, 114.6, 153.7.


1,4-Diethoxy benzene 3c{2,2} (Method B): GC (RI 1250, 95.0%); 1H NMR δ: 1.40 (t, J=6.8 Hz, 6H, CH3), 3.98 (q, J=7.1 Hz, 4H, CH2), 6.84 (s, 4H, ArH); 13C NMR δ: 14.9, 63.9, 115.3, 153.0; IR (cm−1): 2985, 1508, 1394, 1116, 1048, 926, 749, 533.


1,4-Dipropoxy benzene 3c{3,3} (Method B): GC (RI 1434, 96.0%); 1H NMR δ: 1.03 (t, J=7.4 Hz, 6H, CH3), 1.75-1.86 (m, 4H, CH2), 3.87 (q, J=6.5 Hz, 4H, CH2), 6.83 (s, 4H, ArH); 13C NMR δ: 10.5, 22.7, 70.1, 115.4, 153.2; IR (cm−1): 2964, 2876, 1509, 1228, 981, 825, 531.


1,4-Dibutoxy benzene 3c{4,4} (Method B, 6%; Method C, 28%; Method D, 15%, Method E, 80%): GC (RI and ratio) 1849, 99.0%; 1H NMR δ: 0.96 (d, J=6.7 Hz, 12H, CH3), 1.65 (apparent q, J=6.8 Hz, 4H, CH2), 1.78-1.86 (m, 2H, CH), 3.93 (t, J=6.6 Hz, 4H, OCH2), 6.83 (s, 4H, ArH); 13C NMR δ: 22.6, 25.0, 38.1, 67.0, 115.4, 153.2; MS m/z (relative intensity): 223 (M++H, 20%), 222 (M+, 100%); IR (cm−1): 2954, 2871, 1511, 1399, 1237, 1043, 830, 767, 535.


1,4-Di-(3-methyl-butyloxy)benzene 3c{5,5} (Method C, 28%, Method D, 20%): GC (RI and ratio) 1623, 98.0%; 1H NMR δ: 0.98 (d, J=7.4 Hz, 6H, CH3), 1.45-1.53 (m, 4H, CH2), 1.72-1.78 (m, 4H, CH2), 3.91 (t, J=6.5 Hz, 4H, OCH2), 6.83 (s, 4H, ArH); 13C NMR δ: 13.9, 19.2, 31.4, 68.3, 115.3, 153.2; MS m/z (relative intensity): 251 (M++H, 24%), 250 (M+, 100%); IR (cm−1): 2954, 2868, 1509, 1474, 1237, 1061, 821, 740, 523.


1,4-Diallyloxy benzene 3c{6,6} (Method D, 48%): GC (RI and ratio) 1481, 100%; 1H NMR δ: 4.49 (dt, J=5.4, 1.5 Hz, 4H), 5.27-5.30 (m, 2H), 5.39-5.44 (m, 2H), 6.03-6.10 (m, 2H), 6.86 (s, 4H, ArH); 13C NMR δ: 69.36, 115.5, 117.4, 133.5, 152.8; MS m/z (relative intensity): 191 (M++H, 21%), 190 (M+, 100%).


Synthesis of Compounds 4c{3} and 6c{3}


Compound 4c{3} was obtained according to method D in 88% yield. GC (RI and ratio) 1495, 100%; 1H NMR δ: 1.04 (t, J=7.4 Hz, 3H, CH3), 1.76-1.83 (m, 2H), 3.88 (t, J=6.6 Hz, 2H, OCH2), 4.49 (dt, J=5.3, 1.5 Hz, 2H), 5.27-5.30 (m, 1H), 5.39-5.43 (m, 1H), 6.02-6.10 (m, 1H), 6.83-6.87 (m, 4H, ArH); 13C NMR δ: 10.5, 22.6, 69.4, 70.0, 115.3, 115.6, 117.4, 133.6, 152.6, 153.4; MS m/z (relative intensity): 193 (M++H, 48%), 192 (M+, 100%).


The 4c{3} compound (0.3277 g) was heated at 180° C. in a sealed tube, under a nitrogen atmosphere for 5 days. The viscous dark black oil was purified by column chromatography with chloroform to afford 0.1253 g of pure 6c{3} library in 38% yield.


GC (RI and ratio) 1529, 98%; 1H NMR δ: 1.02 (t, J=7.4 Hz, 3H, CH3), 1.45 (d, J=6.3 Hz, 3H, CH3), 1.73-1.80 (m, 2H), 2.77-2.81 (m, 1H), 3.24-3.29 (m, 1H), 3.85 (t, J=6.6 Hz, 2H, OCH2), 4.85-4.92 (m, 1H), 6.64 (d, J=1.5 Hz, 2H, ArH), 6.77 (s, 1H, ArH); 13C NMR δ: 10.5, 21.7, 22.7, 37.6, 70.5, 79.6, 109.0, 112.2, 113.6, 127.9, 153.4, 153.5; MS m/z (relative intensity): 193 (M++H, 27%), 192 (M+, 100%).


The following procedures were used to generate mini-libraries in Set A and Set C as set out in Table 2.


Method F: A mixture of mono-alkoxy phenols (1 eq) in DMF (2 mL) was added to a suspension of NaH (5 eq) in DMF (3 mL). The alkylating reagent (MeI, EtI, PrI, BuBr, bromo-3-methyl butane or allyl bromide, 3 eq) was then added and the reaction mixture was stirred at room temperature and monitored by GC. When reaction was complete (between 1 to 4 h), a solution of saturated NH4Cl (25 mL) was slowly added and the aqueous phase was extracted with CHCl3 (3×20 mL). The combined organic layers were washed with water (4×25 mL) and brine (2×25 mL), dried over anhydrous Na2SO4 and concentrated under reduced pressure. The crude oil was purified by flash column chromatography using hexane:EtOAc (4:1) to afford the corresponding library as pure oil. (Note: the 1,3 dialkoxy benzene libraries required a second purification by flash column chromatography, with hexanes:EtOAc, 4:1).


Method G: A mixture of mono-alkoxy phenols (1 eq) in acetone (5 mL) was added to a suspension of K2CO3 (10 eq) in acetone (20 mL) and the mixture was stirred at room temperature for 2 h. The alkylating reagent (MeI, EtI, PrI, BuBr, 1-bromo-3-methylbutane or allyl bromide, 3 eq) was then added and the reaction mixture was heated at reflux and monitored by GC. When the reaction was complete, the mixture was filtered and the filtrate was concentrated under reduced pressure. The residue obtained was diluted with CHCl3 (30 mL) and water (20 mL). The layers were separated; the organic layer was dried over anhydrous Na2SO4 and concentrated under reduced pressure to afford the corresponding library as pure oil. For compound sets 5b{n,n}, the oils were decolorized with flash chromatography (5% EtOAc in Hexane), even though GC analysis indicated that the compounds were pure.


Method H: A mixture of mono-alkoxy phenols (1 eq) in acetone (5 mL) was added to a suspension of Cs2CO3 (2 eq) in acetone (15 mL) and the mixture was stirred at room temperature for 2 h. The alkylating reagent (3 eq) was then added and the reaction mixture was heated at reflux and monitored by GC. When the reaction was complete, the mixture was filtered and the filtrate was concentrated under reduced pressure. The residue obtained was diluted with CHCl3 (30 mL) and water (20 mL). The layers were separated; the organic layer was dried over anhydrous Na2SO4 and concentrated under reduced pressure to afford the corresponding library as pure oil.


The following data were generated for mini-libraries in Set A and Set C as set out in Table 2.


3a{1,1-5} Methyl library (Method A, 27% yield; Method C, 72% yield): 1H NMR δ: 0.95-0.99 (m, 8.9H), 1.04 (t, J=7.5 Hz, 3H, CH3 (Pr)), 1.45-1.52 (m, 5H), 1.75 (q, J=7.0 Hz, 2H, CH2 (i-Pent)), 1.80-1.91 (m, 5.4H), 3.86, 3.865, 3.87 (s, 8.5H), 3.88 (s, 3H, OCH3 (Me)), 3.89 (s, 6H, OCH3 (Me)), 3.98 (t, J=6.9 Hz, 2H), 4.01-4.06 (m, 4.3H), 4.11 (q, J=7.0 Hz, 2H, OCH2 (Et)), 6.88-6.94 (m, 19H, ArH); GC RI: MS m/z (relative intensity, %): 1,2-dimethoxy benzene 3a{1,1} 1145: 139 (M++H, 29), 138 (M+, 100), 123 (44); 1-ethoxy-2-methoxy benzene 3a{1,2} 1190: 153 (M++H, 23), 152 (M+, 100), 124 (58), 109 (91); 1-methoxy-2-propoxy benzene 3a{1,3} 1280: 167 (M++H, 18), 166 (M+, 100), 124 (66), 109 (76); 1-butoxy-2-methoxy benzene 3a{1,4} 1377: 181 (M++H, 15), 180 (M+, 100), 124 (57), 109 (52); 1-methoxy-2-(3-methyl-butoxy)benzene 3a{1,5} 1434: 195 (M++H, 15), 194 (M+, 100), 124 (68), 109 (46).


3a{2,1-5} Ethyl library (Method A, 57% yield), 3a{3,1-5} propyl library (Method A, 67% yield), 3a{4,1-5} butyl library (Method A, 62% yield), 3a{5,1-5} isopentyl library (Method A, 43% yield), 3a{6,1-5} allyl library (Method B, 94% yield): 1H NMR and GC-MS data:


3a{2,1-5} Ethyl library (Method A, 57% yield): 1H NMR δ: 0.96-0.99 (m, 9.4H), 1.04 (t, J=7.5 Hz, 3H, CH3 (Pr)), 1.41-1.53 (m, 5H), 1.73 (q, J=6.9 Hz, 2H, CH2 (i-Pent)), 1.79-1.89 (m, 5.4H), 3.88 (s, 3H, OCH3 (Me)), 3.97 (t, J=6.8 Hz, 2H), 4.01 (t, J=6.7 Hz, 2H), 4.04 (t, J=6.8 Hz, 2H), 4.05-4.13 (m, 14H), 6.86-6.93 (m, 19H, ArH); GC RI: MS m/z (relative intensity, %): 1,2-diethoxy benzene 3a{2,2} 1244: 167 (M++H, 100), 166 (M+, 81); 1-ethoxy-2-propoxy benzene 3a{2,3} 1335: 181 (M++H, 100), 180 (M+, 60); 1-ethoxy-2-butoxy benzene 3a{2,4} 1429: 195 (M++H, 100), 194 (M+, 83); 1-ethoxy-2-(3-methyl-butoxy)benzene 3a{2,5} 1486: 209 (M++H, 100), 208 (M+, 72).


3a{3,1-5} Propyl library (Method A, 67% yield): 1H NMR δ: 0.96-0.99 (m, 7.4H), 1.04 (t, J=7.4 Hz, 16.5H, CH3 (Pr)), 1.44 (t, J=7.0 Hz, 3H), 1.48-1.53 (m, 1.6H), 1.72 (q, J=6.8 Hz, 1.7H, CH2 (i-Pent)), 1.77-1.91 (m, 14H), 3.87 (s, 3H, OCH3 (Me)), 3.94-4.04 (m, 14.7H), 4.09 (q, J=7.0 Hz, 2H), 6.86-6.92 (m, 17H, ArH); GC RI: MS m/z (relative intensity, %): 1,2-dipropoxy benzene 3a{3,3} 1424: 195 (M++H, 100), 194 (M+, 60); 1-butoxy-2-propoxy benzene 3a{3,4} 1518: 209 (M++H, 100), 208 (M+, 84); 1-(3-methyl-butoxy)-2-propoxy benzene 3a{3,5} 1576: 223 (M++H, 100), 222 (M+, 62).


3a{4,1-5} Butyl library (Method A, 62% yield): 1H NMR δ: 0.96-0.99 (m, 24H), 1.04 (t, J=7.5 Hz, 3.4H, CH3 (Pr)), 1.44 (t, J=7.0 Hz, 3H), 1.46-1.54 (m, 12.6H), 1.71 (q, J=6.8 Hz, 2H, CH2 (i-Pent)), 1.77-1.88 (m, 16H), 3.87 (s, 3H, OCH3 (Me)), 3.96 (t, J=6.6 Hz, 2H), 3.98-4.05 (m, 15H), 4.07 (q, J=7.0 Hz, 2.4H), 6.82-6.94 (m, 20H, ArH); GC RI: MS m/z (relative intensity, %): 1,2-dibutoxy benzene 3a{4,4} 1608: 223 (M++H, 100), 222 (M+, 64); 1-butoxy-2-(3-methyl-butoxy)benzene 3a{4,5} 1664: 237 (M++H, 100), 236 (M+, 64).


3a{5,1-5} Isopentyl library. (Method A, 43% yield): 1H NMR δ: 0.96-0.99 (m, 41H), 1.04 (t, J=7.5 Hz, 3.4H, CH3 (Pr)), 1.43 (t, J=7.0 Hz, 3.8H), 1.50 (q, J=7.5 Hz, 2.6H), 1.69-1.90 (m, 24H), 3.86 (s, 3H, OCH3 (Me)), 3.96 (t, J=6.6 Hz, 2H), 3.98-4.10 (m, 18H), 6.84-6.93 (m, 20H, ArH); GC RI: MS m/z (relative intensity, %): 1,2-di(3-methyl-butoxy)benzene 3a{5,5} 1720: 251 (M++H, 20), 250 (M+, 100).


3a{6,1-5} Allyl library. (Method B, 94% yield): 1H NMR δ: 0.96-1.00 (m, 7H), 1.05 (t, J=7.4 Hz, 3H, CH3 (Pr)), 1.45 (t, J=7.0 Hz, 3.7H), 1.49-1.53 (m, 1.7H), 1.73 (q, J=6.9 Hz, 1.4H), 1.79-1.89 (m, 4.6H), 3.88 (s, 4H, OCH3 (Me)), 3.98 (t, J=6.7 Hz, 1.8H), 4.01-4.06 (m, 3.3H), 4.10 (q, J=7.0 Hz, 2.4H), 4.58-4.63 (m, 10.6H), 5.25-5.30 (m, 5.1H), 5.38-5.44 (m, 5H), 6.04-6.14 (m, 5H), 6.84-6.95 (m, 21H, ArH); GC RI: MS m/z (relative intensity, %): 1-allyloxy-2-methoxy benzene 3a{6,1} 1281: 165 (M++H, 42), 164 (M+, 100); 1-allyloxy-2-ethoxy benzene 3a{6,2} 1327: 179 (M++H, 100), 178 (M+, 67); 1-allyloxy-2-propoxy benzene 3a{6,3} 1416: 193 (M++H, 100), 192 (M+, 91); 1-allyloxy-2-butoxy benzene 3a{6,4} 1510: 207 (M++H, 100), 206 (M+, 72); 1-allyloxy-2-(3-methyl-butoxy) benzene 3a{6,5} 1569: 221 (M++H, 100), 220 (M+, 70).


3b{1,1-5} Methyl library (Method A, 85% yield): 1H NMR δ: 0.97 (d, J=6.6 Hz, 6H, CH3 (i-Pent)), 1.04 (t, J=7.4 Hz, 3H, CH3 (Pr)), 1.42 (t, J=7.0 Hz, 3H, CH3 (Et)), 1.68 (apparent q, J=6.8 Hz, 2H, CH2 (i-Pent)), 1.78-1.87 (m, 3H), 3.79-3.80 (m, 15H, OCH3), 3.91 (t, J=6.6 Hz, 2H, OCH2 (Pr)), 3.98 (t, J=6.7 Hz, 2H, OCH2 (i-Pent)), 4.02 (q, J=7.0 Hz, 2H, OCH2 (Et)), 6.47-6.53 (m, 9.6H, ArH), 7.18 (t, J=8.2 Hz, 3H, ArH); GC RI: MS m/z (relative intensity, %): 1,3-dimethoxy benzene 3b{1,1} 1180: 138 (M+, 100); 1-ethoxy-3-methoxy benzene 3b{1,2} 1253: 153 (M++H, 25), 152 (M+, 100); 1-methoxy-3-propoxy benzene 3b{1,3} 1345: 167 (M++H, 32), 166 (M+, 100), 124 (22); 1-methoxy-3-(3-methyl-butyloxy)benzene 3b{1,5} 1508: 195 (M++H, 30), 194 (M+, 100).


3b{2,1-5} Ethyl library (Method A, 66% yield), 3b{3,1-5} propyl library (Method A, 53% yield), 3b{4,1-5} butyl library (Method A, 69% yield) 3b{5,1-5} isopentyl library (Method A, 72% yield), 3b {6,1} (Method B, % yield), 3b {6,2-3} (Method B, % yield), 3b {6, 4-5} (Method B, % yield) 1H NMR and GC-MS data:


3b{2,1-5} Ethyl library. (Method A, 66% yield): 1H NMR δ: 0.96 (d, J=6.6 Hz, 6H, CH3 (i-Pent)), 1.03 (t, J=7.4 Hz, 3H, CH3 (Pr)), 1.39-1.43 (m, 12.8H), 1.67 (apparent q, J=6.8 Hz, 2H, CH2 (i-Pent)), 1.78-1.84 (m, 3H), 3.79 (s, 3H, CH3 (Me)), 3.90 (t, J=6.6 Hz, 2H, OCH2 (Pr)), 3.98 (t, J=6.7 Hz, 2H, OCH2 (i-Pent)), 3.99-4.04 (m, 8H), 6.46-6.51 (m, 10H, ArH), 7.16 (t, J=8.2 Hz, 3H, ArH); GC RI: MS m/z (relative intensity, %): 1,3-diethoxy benzene 3b{2,2} 1318: 167 (M++H, 31), 166 (M+, 100); 1-ethoxy-3-propoxy benzene 3b{2,3} 1409: 181 (M++H, 40), 180 (M+, 100); 1-ethoxy-3-(3-methyl-butyloxy) benzene 3b{2,5} 1570: 209 (M++H, 35), 208 (M+, 100).


3b{3,1-5} Propyl library (Method A, 53% yield): 1H NMR δ: 0.96 (d, J=6.6 Hz, 6H, CH3 (i-Pent)), 1.02-1.05 (m, 10H), 1.40 (t, J=7.0 Hz, 2H, CH3 (Et)), 1.67 (apparent q, J=6.8 Hz, 2H, CH2 (i-Pent)), 1.77-1.85 (m, 8H), 3.79 (s, 1.5H, OCH3 (Me)), 3.89-3.92 (m, 7H), 3.97 (t, J=6.7 Hz, 2H, OCH2 (i-Pent)), 4.02 (q, J=7.0 Hz, 1.2H, OCH2 (Et)), 6.46-6.51 (m, 7H, ArH), 7.16 (t, J=8.2 Hz, 2.5H, ArH); GC RI: MS m/z (relative intensity, %): 1,3-dipropoxy benzene 3b{3,3} 1501: 195 (M++H, 45), 194 (M+, 100), 110 (85), 82(22); 1-(3-methyl-butyloxy)-3-propoxy benzene 3b{3,5} 1657: 223 (M++H, 44), 222 (M+, 100).


3b{4,1-5} Butyl library (Method A, 69% yield): 1H NMR δ: 0.99-1.02 (m, 19H), 1.05 (t, J=7.0 Hz, 3H, CH3 (Pr)), 1.41-1.45 (m, 3H), 1.48-1.56 (m, 8H), 1.70 (apparent q, J=6.7 Hz, 2.5H, CH2 (i-Pent)), 1.76-1.91 (m, 8H), 3.81 (s, 3H, OCH3 (Me)), 3.93 (t, J=6.6 Hz, 2H, OCH2 (Pr)), 3.95-4.01 (m, 11H), 4.03 (q, J=7.0 Hz, 2H, OCH2 (Et)), 6.45-6.54 (m, 11.7H, ArH), 7.16 (t, J=8.2 Hz, 4H, ArH); GC RI: MS m/z (relative intensity, %): 1-butoxy-3-methoxy benzene 3b{4,1} 1440: 181 (M++H, 25), 180 (M+, 100); 1-butoxy-3-ethoxy benzene 3b{4,2} 1506: 193 (M++H, 33), 194 (M+, 100); 1-butoxy-3-propoxy benzene 3b{4,3} 1596: 209 (M++H, 48), 208 (M+, 100); 1-butoxy-3-(3-methyl-butyloxy)benzene 3b{4,5} 1754: 237 (M++H, 42), 236 (M+, 100).


3b{5,1-5} Isopentyl library. (Method A, 72% yield): 1H NMR δ: 0.99 (d, J=6.7 Hz, 26H), 1.06 (t, J=7.4 Hz, 3H, CH3 (Pr)), 1.41-1.45 (m, 3H), 1.70 (apparent q, J=6.7 Hz, 9H), 1.81-1.88 (m, 6.3H), 3.81 (s, 3H, OCH3 (Me)), 3.92 (t, J=6.6 Hz, 2H, OCH2 (Pr)), 3.98-4.04 (m, 11H), 6.50-6.54 (m, 11H, Art), 7.18 (t, J=8.2 Hz, 4H, ArH); GC RI: MS m/z (relative intensity, %): 1-methoxy-3-(3-methyl-butyloxy)benzene 3b{5,1} 1500: 195 (M++H, 26), 194 (M+, 100); 1-ethoxy-3-(3-methyl-butyloxy)benzene 3b{5,2} 1566: 209 (M++H, 35), 208 (M+, 100); 1-(3-methyl-butyloxy)-3-propoxy benzene 3b{5,3} 1653: 223 (M++H, 48), 222 (M+, 100); 1,3-di(3-methyl-butyloxy)benzene 3b{5,5} 1826: 251 (M++H, 40), 250 (M+, 100).


The meta allyl library was synthesized in three portions (methyl by itself, ethyl+propyl and butyl+isopentyl), because upon Claisen rearrangement each compound gave rise to two rearrangement products.


3b{6,1} 1-allyloxy-3-methoxybenzene. (Method D, 98% yield): 1H NMR δ: 3.80 (s, 3H, CH3), 4.53 (apparent d, J=5.5 Hz, 2H, allyl CH2), 5.30 (apparent d, J=14 Hz, 1H), 5.43 (apparent d, J=22 Hz, 1H), 6.07 (m, 1H), 6.52 (m, 3H, ArH), 7.19 (apparent t, J=7.7 Hz, 1H ArH). GC RI: 1334 MS m/z (relative intensity, %): 164 (M+, 100), 149 (M−CH3, 10), 136 (M−28, 12).


3b{6,2-3} Allyl library (ethyl, propyl). (Method D, 60% yield, 35% 3b{6,2} by GC and 39% by 1H NMR and the rest is 3b {6,3}): 1H NMR δ: 1.04 (t, J=4 Hz, 3H, CH3 propyl), 1.42 (t, J=3.7 Hz, 3H, CH3 ethyl), 1.81 (m, 2H, CH2, propyl), 3.95 (t, J=3.7 Hz, 2H propyl CH2), 4.02 (q, J=7 Hz, 2H, ethyl), 4.53 (apparent d, J=7 Hz, 2H for each component), 5.29 (m, J=14 Hz, 1H for each component), 5.41 (m, J=22 Hz, 1H for each component), 6.07 (m, 1H for each component), 6.53 (m, 3H for each component), 7.17 (apparent t, J=8 Hz, 1H for each component). GC RI: MS m/z (relative intensity, %): 1-allyloxy-3-ethoxybenzene 3b {6,2} 1398: 179 (M+1, 72), 178 (M+, 100), 150 (M−28, 35); 1-allyloxy-3-propoxybenzene 3b {6,3} 1491: 193 (M+1, 93), 192 (M+, 100), 164 (M−28, 12), 150 (31).


3b {6,4-5} Allyl library (butyl, isopentyl). (Method D, 71% yield, 3b (6,4) 34% by GC and 40% by 1H NMR and the rest is 3b {6,5}): 1H NMR δ: 0.98 (m, 6H, CH3 isopentyl, 3H CH3 butyl), 1.48 (m, 2H, CH2 butyl), 1.68 (m, 2H, CH2, isopentyl), 1.75 (m, 2H, CH2, butyl), 1.83 (m, 1H, isopentyl), 3.96 (m, 2H for each component, CH2), 4.52 (apparent d, J=8 Hz, 2H for each component), 5.29 (apparent d, J=14 Hz, 1H for each component), 5.42 (apparent d, J=22 Hz, 1H for each component), 6.06 (m, 1H for each component), 6.51 (m, 3H for each component, ArH), 7.17 (apparent t, J=7 Hz, 1H for each component, ArH). GC RI: MS m/z (relative intensity, %): 1-allyloxy-3-n-butoxybenzene 3b {6,4} 1592: 207 (M+1, 83), 206 (M+, 100), 178 (M−28, 12), 150 (33). 1-allyloxy-3-isopentyloxybenzene 3b {6,5} 1654: 221 (M+1, 81), 220 (M+, 100), 192 (M−28, 7), 150 (21).


3c{1,1-5} Methyl library (Method A, 65% yield): 1H NMR δ. 0.96 (d, J=6.7 Hz, 9H, CH3 (i-Pent)), 1.03 (t, J=7.4 Hz, 3.3H, CH3 (Pr)), 1.39 (t, J=6.7 Hz, 4H, CH3 (Et)), 1.66 (apparent q, J=6.7 Hz, 3H, CH2 (i-Pent)), 1.77-1.85 (m, 3H), 3.77, 3.78 (s, 15H, OCH3), 3.87 (t, J=6.6 Hz, 2H, CH2 (Pr)), 3.94 (t, J=6.6 Hz, 3H, CH2 (i-Pent)), 3.98 (q, J=7.1 Hz, 3H, CH2 (Et)), 6.83-6.85 (m, 16H, ArH); GC RI: MS m/z (relative intensity, %): 1,4-dimethoxy benzene 3c{1,1} 1122: 139 (M++H, 80), 138 (M+, 100); 1-ethoxy-4-methoxy benzene 3c{1,2} 1188: 153 (M++H, 73), 152 (M+, 100); 1-methoxy-4-propoxy-benzene 3c{1,3} 1281: 167 (M++H, 48), 166 (M+, 100); 1-methoxy-4-(3-methyl-butyloxy)benzene 3c{1,5} 1442: 195 (M++H, 48), 194 (M+, 100).


3c{2,1-5} Ethyl library (Method A, 31% yield), 3c{3,1-5} propyl library (Method A, 82% yield), 3c{4,1-5} butyl library (Method A, 76% yield), 3c{5,1-5} isopentyl (3-methyl-butyloxy) library (Method A, 82% yield), 3c{6,1-5} allyl library (Method B, 95% yield); 1H NMR and GC-MS data:


3c{2,1-5} Ethyl library (Method A, 31% yield): 1H NMR δ. 0.96 (d, J=6.7 Hz, 6H, CH3 (i-Pent)), 1.03 (t, J=7.4 Hz, 3H, CH3 (Pr)), 1.39 (t, J=7.0 Hz, 15H, CH3 (Et)), 1.66 (apparent q, J=6.8 Hz, 3H, CH2 (i-Pent)), 1.77-1.83 (m, 4H), 3.77 (s, 3H, OCH3 (Me)), 3.87 (t, J=6.6 Hz, 2H, OCH2 (Pr)), 3.94 (t, J=6.7 Hz, 2H, OCH2 (i-Pent)), 3.98 (q, J=7.0 Hz, 10H, OCH2 (Et)), 6.83-6.84 (m, 16H, ArH); GC RI: MS m/z (relative intensity, %): 1,4-diethoxy benzene 3c{2,2} 1248: 167 (M++H, 33), 166 (M+, 100); 1-ethoxy-4-propoxy benzene 3c{2,3} 1337: 181 (M++H, 28), 180 (M+, 100); 1-ethoxy-4-(3-methyl-butyloxy) benzene 3c{2,5} 1492: 209 (M++H, 31), 208 (M+, 100).


3c{3,1-5} Propyl library (Method A, 82% yield): 1H NMR δ: 0.97 (d, J=6.6 Hz, 7H, CH3 (i-Pent)), 1.03 (t, J=7.4 Hz, 15H, CH3 (Pr)), 1.40 (t, J=6.9 Hz, 3H, CH3 (Et)), 1.66 (apparent q, J=6.8 Hz, 3H, CH2 (i-Pent)), 1.77-1.85 (m, 12H), 3.77 (s, 3H, OCH3 (Me)), 3.87 (t, J=6.5 Hz, 10H, OCH2 (Pr)), 3.94 (t, J=6.6 Hz, 2.8H, OCH2 (i-Pent)), 3.98 (q, J=7.1 Hz, 2H, OCH2 (Et)), 6.83-6.84 (m, 16H, ArH); GC RI: MS m/z (relative intensity, %): 1,4-dipropoxy benzene 3c{3,3} 1431: (M++H, 25), 194 (M+, 100); 1-(3-methyl-butyloxy)-4-propoxy benzene 3c{3,5} 1589: 223 (M++H, 28), 222 (M+, 100).


3c{4,1-5} Butyl library (Method A, 76% yield): 1H NMR δ. 0.96-0.99 (m, 18H), 1.02 (t, J=7.4 Hz, 3H, CH3 (Pr)), 1.39 (t, J=6.9 Hz, 4H, CH3 (Et)), 1.45-1.53 (m, 8H, CH2 (Bu)), 1.66 (apparent q, J=6.7 Hz, 2H, CH2 (i-Pent)), 1.72-1.81 (m, 11.6H), 3.77 (s, 3H, OCH3 (Me)), 3.87 (t, J=6.6 Hz, 2H, OCH2 (Pr)), 3.90-3.95 (m, 10.4H), 3.98 (q, J=7.0 Hz, 2H, OCH2 (Et)), 6.83-6.84 (m, 16H, ArH); GC RI: MS m/z (relative intensity, %): 1-butoxy-4-methoxy benzene 3c{4,1} 1371: 181 (M++H, 29), 180 (M+, 100); 1-butoxy-4-ethoxy benzene 3c{4,2} 1437: 195 (M++H, 23), 194 (M+, 100); 1-butoxy-4-propoxy benzene 3c{4,3} 1529: 209 (M++H, 40), 208 (M+, 100); 1-butoxy-4-(3-methyl-butyloxy)benzene 3c{4,5} 1681: 237 (M++H, 42), 236 (M+, 100).


3c{5,1-5} Isopentyl (3-methyl-butyloxy) library. (Method A, 82% yield): 1H NMR δ. 0.96 (d, J=7.0 Hz 30H, CH3 (i-Pent)), 1.02 (t, J=7.4 Hz, 3H, CH3 (Pr)), 1.39 (t, J=6.9 Hz, 3H, CH3 (Et)), 1.66 (apparent q, J=6.8 Hz, 10H, CH2 (i-Pent)), 1.75-1.86 (m, 7.5H), 3.77 (s, 3H, OCH3 (Me)), 3.87 (t, J=6.4 Hz, 2H, OCH2 (Pr)), 3.94 (t, J=6.9 Hz, 10H, OCH2 (i-Pent)), 3.98 (q, J=6.8 Hz, 2H, OCH2 (Et)), 6.83-6.84 (m, 16H, ArH); GC RI: MS m/z (relative intensity, %): 1,4-di(3-methyl-butyloxy)-benzene 3c{5,5} 1850: 251 (M++H, 25), 250 (M+, 100).


3c{6,1-5} Allyl library. (Method B, 95% yield): GC (RI): 1H NMR δ: 0.95-0.98 (m, 8H), 1.03 (t, J=7.4 Hz, 3H, CH3 (Pr)), 1.39 (t, J=7.0 Hz, 3.9H, CH3 (Et)), 1.46-1.50 (m, 1.5H), 1.56 (d, J=3.8 Hz, 1.3H), 1.65 (apparent q, J=6.8 Hz, 2H, CH2 (i-Pent)), 1.71-1.85 (m, 5H), 3.78 (s, 4H, OCH3 (Me)), 3.87 (t, J=6.6 Hz, 2H, OCH2 (Pr)), 3.90-3.95 (m, 3.7H), 3.98 (q, J=7.0 Hz, 2.5H, OCH2 (Et)), 4.47-4.49 (m, 10.9H), 5.25-5.29 (m, 5.H), 5.38-5.42 (m, 5H), 6.01-6.09 (m, 5H), 6.81-6.87 (m, 21H, ArH); GC RI: MS m/z (relative intensity, %): 1-allyloxy-4-methoxy benzene 3c{6,1} 1326: 165 (M++H, 20), 164 (M+, 100); 1-allyloxy-4-ethoxy benzene 3c{6,2} 1394: 179 (M++H, 70), 178 (M+, 100); 1-allyloxy-4-propoxy benzene 3c{6,3} 1491: 193 (M++H, 65), 192 (M+, 100); 1-allyloxy-4-butoxy benzene 3c{6,4} 1594: 207 (M++H, 56), 206 (M+, 100); 1-allyloxy-4-(3-methyl-butoxy) benzene 3c{6,5}1659: 221 (M++H, 46), 220 (M+, 100).


The following procedures were used to generate mini-libraries in Set B as set out in Table 2.


The allyloxy-alkoxy mini-library 3(a-c){6,1-5} was heated at 180° C. in a sealed tube, under a nitrogen atmosphere. Reaction progress was monitored by GC. In order to remove the color, the crude libraries were passed through a silica column (top charcoal layer, chloroform as eluent).


The following data were generated for mini-libraries in Set B


4a{1-5}95% yield: 1H NMR δ: 0.97-1.00 (m, 7.3H), 1.05 (t, J=7.4 Hz, 3H, CH3 (Pr)), 1.44 (t, J=7.0 Hz, 3.8H, CH3 (Et)), 1.50 (q, J=7.6 Hz, 1.7H), 1.71 (apparent q, J=6.8 Hz, 1.5H, CH2 (i-Pent)), 1.77-1.88 (m, 4.4H), 3.42 (d, J=6.6 Hz, 9.3H), 3.89 (s, 3.7H, OCH3 (Me)), 3.99 (t, J=6.5 Hz, 2H, OCH2 (Pr)), 4.02-4.07 (m, 3.8H), 4.10 (q, J=7.0 Hz, 2.7H, OCH2 (Et)), 5.04-5.11 (m, 10.2H), 1.69 (s, 1.2H, OH), 5.73 (s, 0.5H, OH), 5.74 (s, 0.8H, OH), 5.75 (s, 1.8H, OH), 5.98-6.06 (m, 4H), 6.70-6.86 (m, 13.8H, ArH); GC RI: MS m/z (relative intensity, %): 2-allyl-6-methoxy phenol 4a{1} 1358: 165 (M++H, 23), 164 (M+, 100); 2-allyl-6-ethoxy phenol 4a{2} 1413: 179 (M++H, 25), 178 (M+, 100); 2-allyl-6-propoxy phenol 4a{3} 1504: 193 (M++H, 22), 192 (M+, 100); 2-allyl-6-butoxy phenol 4a{4} 1603: 207 (M++H, 22), 206 (M+, 100); 2-allyl-6-(3-methyl-butoxy)phenol 4a{5} 1664: 221 (M++H, 21), 220 (M+, 100).


4bx,y{1} 82% yield: 1H NMR δ: 3.35 (m, 3.8H, CH2 (Allylx), 3.47 (m, 2H, CH2 (Allyly), 3.77 (s, 6.6H, OCH3 (Mex)), 3.81 (s, 3H, OCH3 (Mey)), 5.01 (s, 1H, OHy), 5.04 (s, 1.7H, OHx), 5.08-5.13 (m, 2.1H), 5.14-5.18 (m, 3.8H), 5.95-6.04 (m, 2.6H), 6.42 (d, J=2.5 Hz, 1.7H, ArHx), 6.46 (dd, J=2.5 and 8.3 Hz, 1.7H, ArHx), 6.50 (dd, J=6.5 and 7.9 Hz, 2H, ArHy), 7.00 (d, J=8.3 Hz, 1.7H, ArHx), 7.08 (t, J=8.2 Hz, 1H, ArHy); GC RI: MS m/z (relative intensity, %): 2-allyl-5-methoxy phenol 4bx{1} 1393: 165 (M++H, 30), 164 (M+, 100); 2-allyl-3-methoxy phenol 4by{1} 1446: 165 (M++H, 37), 164 (M+, 100).


4bx,y{2-3} 64% yield: 1H NMR δ: 1.01-1.06 (m, 10.9H, CH3 (Pr)), 1.38-1.42 (m, 7.7H, CH3 (Et)), 1.75-1.84 (m, 7.6H, CH2CH3 (Pr)), 3.34-3.35 (m, 7.3H), 3.47-3.49 (m, 4.2H), 3.86-3.92 (m, 7.5H, OCH2 (Pr)), 3.97-4.04 (m, 5.4H, OCH2 (Et)), 5.06-5.09 (m, 7.4H), 5.11-5.12 (m, 1.3H), 5.13-5.15 (m, 6.5H), 5.17-5.18 (m, 2H), 5.94-6.04 (m, 5.6H), 6.41-6.49 (m, 11.5H, ArH), 6.98 (m, 3.5H, ArHx), 7.05 (m, 2H, ArHy); GC RI: MS m/z (relative intensity, %): 2-allyl-5-ethoxy phenol 4bx{2} 1455: 179 (M++H, 54), 178 (M+, 100); 2-allyl-3-ethoxy phenol 4by{2} 1517: 179 (M++H, 38), 178 (M+, 100); 2-allyl-5-propoxy phenol 4bx{3} 1549: 193 (M++H, 62), 192 (M+, 100); 2-allyl-3-propoxy phenol 4by{3} 1615: 193 (M++H, 47), 192 (M+, 100).


4bx,y{4-5} 31% yield: 1H NMR δ: 0.94-0.99 (m, 30.1H), 1.44-1.53 (m, 8.5H, CH2CH3 (Bu)), 1.61-1.87 (m, 17.6H), 3.34-3.35 (m, 9.5H), 3.46-3.48 (m, 4.4H), 3.90-3.98 (m, 14.6H), 5.01-5.03 (m, 6.0H), 5.06-5.09 (m, 2.2H), 5.10-5.12 (m, 1.1H), 5.13-5.18 (m, 10.1H), 5.93-6.04 (m, 6.1H), 6.41-6.50 (m, 13.6H), 6.97-6.98 (m, 4.4H, ArHx), 7.03-7.07 (m, 2H, ArHy); GC RI: MS m/z (relative intensity, %): 2-allyl-5-butoxy phenol 4bx{4} 1649: 207 (M++H, 11), 206 (M+, 54), 135 (M−71, 100); 2-allyl-3-butoxy phenol 4by{4} 1721: 207 (M++H, 17), 206 (M+, 94), 149 (M−57, 100); 2-allyl-5-isopentoxy phenol 4bx{5} 1706: 221 (M++H, 11), 220 (M+, 54), 135 (M−85, 100); 2-allyl-3-(3-methyl-butoxy)phenol 4by{5} 1786: 221 (M++H, 17), 220 (M+, 90), 150 (M−70, 100).


4c{1-5}97% yield: 1H NMR δ: 0.97-0.99 (m, 8.8H), 1.03 (t, J=7.4 Hz, 3.7H, CH3 (Pr)), 1.39 (t, J=7.0 Hz, 4.2H, CH3 (Et)), 1.45-1.53 (m, 2H), 1.66 (apparent q, J=6.8 Hz, 2H, CH2 (i-Pent)), 1.72-1.86 (m, 5.4H), 3.37-3.40 (m, 11.5H), 3.77 (s, 4.3H, OCH3 (Me)), 3.86 (t, J=6.6 Hz, 2.5H, OCH2 (Pr)), 3.89-3.95 (m, 3.5H), 3.98 (q, J=7.0 Hz, 2.6H, OCH2 (Et)), 5.21 (broad s, 5.2H, OH), 5.13-5.17 (m, 10.6H), 5.98-6.06 (m, 5H), 6.66-6.77 (m, 16H, ArH); GC RI: MS m/z (relative intensity, %): 2-allyl-4-methoxy phenol 4c{1} 1432: 165 (M++H, 31), 164 (M+, 100); 2-allyl-4-ethoxy phenol 4c{2} 1494: 179 (M++H, 31), 178 (M+, 100); 2-allyl-4-propoxy phenol 403) 1587: 193 (M++H, 31), 192 (M+, 100); 2-allyl-4-butoxy phenol 4c{4} 1687: 207 (M++H, 29), 206 (M+, 100); 2-allyl-4-(3-methyl-butoxy) phenol 4c[5] 1750: 221 (M++H, 31), 220 (M+, 100).


The following data were generated for Set C


5a{1,1-5} Allyl-methyl library. (Method B, 90% yield): 1H NMR δ: 0.96-1.00 (m, 8.4H), 1.06 (t, J=7.4, 3.2H, CH3 (Pr)), 1.44-1.47 (m, 4.4H), 1.50-1.55 (m, 2.3H), 1.73 (apparent q, J=6.8 Hz, 1.7H, CH2 (i-Pent)), 1.79-1.91 (m, 5.9H), 3.40-3.43 (m, 10H), 3.81, 3.82, 3.83, 3.834, 3.84 (s, 15.2H, OCH3), 3.86 (s, 4.8H, OCH3), 3.95 (t, J=6.5 Hz, 2H, OCH2 (Pr)), 3.98-4.03 (m, 4.4H), 4.07 (q, J=7.0 Hz, 2.7H, OCH2 (Et)), 5.02-5.09 (m, 10H), 5.94-6.02 (m, 5H), 6.71-6.82 (m, 12H, ArH), 6.95-7.01 (m, 5H, Art); GC RI: MS m/z (relative intensity, %): 1-allyl-2,3-dimethoxy benzene 5a{1,1} 1333: 179 (M++H, 50), 178 (M+, 100); 1-allyl-3-ethoxy-2-methoxy benzene 5a{1,2} 1386: 193 (M++H, 79), 192 (M+, 100); 1-allyl-2-methoxy-3-propoxy benzene 5a{1,3} 1481: 207 (M++H, 65), 206 (M+, 100); 1-allyl-2-butoxy-3-methoxy benzene 5a{1,4} 1578: 221 (M++H, 66), 220 (M+, 100); 1-allyl-2-methoxy-3-(3-methyl-butoxy)benzene 5a{1,5} 1632: 235 (M++H, 62), 234 (M+, 100).


5a{2,1-5} Allyl-ethyl library (Method B, 91% yield), 5a{3,1-5} allyl-propyl library (Method B, 96% yield), 5a{4,1-5} allyl-butyl library (Method B, 92% yield), 5a{5,1-5} allyl-iPentyl library (Method B, 90% yield), 5a{6,1-5} allyl-allyl library (Method B, 90% yield); 1H NMR and GC-MS data:


5a{2, 1-5} Allyl-ethyl library. (Method B, 91% yield): 1H NMR δ: 0.97-1.00 (m, 11.5H), 1.35-1.40 (m, 14.8H), 1.42-1.16 (m, 10.5H), 1.72 (apparent q, J=6.7 Hz, 1.8H, CH2 (i-Pent)), 1.78-1.91 (m, 5.7H), 3.43 (d, J=6.6 Hz, 9.2H), 3.84 (s, 3.9H, OCH3), 3.91-4.12 (m, 20.9H), 5.01-5.10 (m, 10.3H), 5.94-6.02 (m, 5H), 6.69-6.83 (m, 11.4H, ArH), 6.90-7.00 (m, 6.7H, ArH); GC RI: MS m/z (relative intensity, %): 1-allyl-2,3-diethoxy benzene 5a{2,2} 1435: 207 (M++H, 63), 206 (M+, 100); 1-allyl-2-ethoxy-3-propoxy benzene 5a{2,3} 1523: 221 (M++H, 56), 220 (M+, 100); 1-allyl-2-butoxy-3-ethoxy benzene 5a{2,4} 1616: 235 (M++H, 88), 234 (M+, 100); 1-allyl-2-ethoxy-3-(3-methyl-butoxy)benzene 5a{2,5} 1669: 249 (M++H, 79), 248 (M+, 100).


5a{3,1-5} Allyl-propyl library. (Method B, 96% yield): 1H NMR δ: 0.97-1.08 (m, 27.8H), 1.44 (t, J=7.0 Hz, 4H), 1.49-1.56 (m, 2.3H), 1.69-1.89 (m, 16.3H), 3.42 (d, J=6.6 Hz, 9.5H), 3.84 (s, 4H, OCH3), 3.86-4.09 (m, 21H), 5.02-5.08 (m, 10H), 5.94-6.02 (m, 5H), 6.69-6.83 (m, 11.4H, ArH), 6.89-6.99 (m, 6H, ArH); GC RI: MS m/z (relative intensity, %): 1-allyl-2,3-dipropoxy benzene 5a{3,3} 1608: 235 (M++H, 57), 234 (M+, 100); 1-allyl-3-butoxy-2-propoxy benzene 5a{3,4} 1699: 249 (M++H, 100), 248 (M+, 72); 1-allyl-3-(3-methyl-butoxy)-2-propoxy benzene 5a{3,5} 1751: 263 (M++H, 50), 262 (M+, 90), 249 (100).


5a{4,1-5} Allyl-butyl library. (Method B, 92% yield): 1H NMR δ: 0.96-0.99 (m, 22.3H), 1.05 (t, J=7.4 Hz, 2.7H), 1.43 (t, J=6.9 Hz, 4.2H), 1.47-1.54 (m, 12.2H), 1.69-1.89 (m, 16.7H), 3.42 (d, J=6.6 Hz, 9.2H), 3.84 (s, 4H, OCH3), 3.88-4.11 (m, 19H), 5.02-5.10 (m, 10.3H), 5.93-6.01 (m, 5H), 6.69-6.83 (m, 11.4H, ArH), 6.89-6.99 (m, 6.5H, ArH); GC RI: MS m/z (relative intensity, %): 1-allyl-2,3-dibutoxy benzene 5a{4,4} 1784: 263 (M++H, 27), 262 (M+, 100); 1-allyl-2-butoxy-3-(3-methyl-butoxy)benzene 5a{4,5} 1833: 277 (M++H, 25), 276 (M+, 100).


5a{5,1-5} Allyl-iPentyl library. (Method B, 90% yield): 1H NMR δ: 0.95-1.00 (m, 37.6H), 1.06 (t, J=7.5 Hz, 2.7H), 1.44 (t, J=7.0 Hz, 4.3H), 1.49-1.55 (m, 2.1H), 1.65-1.72 (m, 12.4H), 1.78-1.90 (m, 10H), 3.41 (d, J=6.6 Hz, 9.4H), 3.84 (s, 4H, OCH3), 3.91-4.08 (m, 19H), 5.01-5.09 (m, 10.2H), 5.93-6.01 (m, 5H), 6.69-6.83 (m, 11.1H, ArH), 6.89-6.99 (m, 7.2H, ArH); GC RI: MS m/z (relative intensity, %): 1-allyl-2,3-di(3-methyl-butoxy) benzene 5a{5,5} 1879: 291 (M+H, 23), 290 (M+, 100).


5a{6,1-5} Allyl-allyl library. (Method B, 90% yield): 1H NMR δ: 0.88-0.93 (m, 11.3H), 0.96-1.01 (m, 2.7H), 1.36-1.39 (m, 2.7H), 1.45 (t, J=7.3 Hz, 2.7H), 1.65 (q, J=6.7 Hz, 2.3H), 1.70-1.83 (m, 6H), 3.25 (d, J=7.0 Hz, 1.5H), 3.35 (d, J=6.6 Hz, 8.7H), 3.78 (s, 2.5H, OCH3), 3.86-4.04 (m, 9.5H), 4.40-4.54 (m, 10.3H), 4.95-5.02 (m, 10.2H), 5.13-5.20 (m, 5H), 5.27-5.36 (m, 5H), 5.84-5.92 (m, 5H), 5.97-6.08 (m, 5H), 6.61-6.76 (m, 11.1H, ArH), 6.82-6.94 (m, 5H, ArH); GC RI: MS m/z (relative intensity, %): 1-allyl-2-allyloxy-3-methoxy benzene 5a{6,1} 1463: 205 (M++H, 84), 204 (M+, 100); 1-allyl-2-allyloxy-3-ethoxy benzene 5a{6,2} 1509: 219 (M++H, 100), 218 (M+, 95); 1-allyl-2-allyloxy-3-propoxy benzene 5a{6,3} 1597: 233 (M++H, 100), 232 (M+, 87); 1-allyl-2-allyloxy-3-butoxy benzene 5a{6,4} 1688: 247 (M++H, 100), 246 (M+, 91); 1-allyl-2-allyloxy-3-(3-methyl-butoxy)benzene 5a{6,5} 1740: 261 (M++H, 100), 260 (M+, 92).


5bx,y{1,1} Allyl-methyl library A. (Method B, 90% yield): 1H NMR δ: 3.30-3.31 (m, 3.9H, CH2 (Allylx), 3.41 (dt, J=1.6 and 6.1 Hz, 2H, CH2 (Allyly)), 3.79 (s, 6H (Mey)), 3.80 (s, 5.4H (Mex)), 3.81 (s, 5.4H (Mex)), 4.91-4.95 (m, 1.6H), 4.97-5.04 (m, 4.2H), 5.91-6.01 (m, 2.8H), 6.42-6.45 (m, 3.7H, ArHx), 6.55 (d, J=8.3 Hz, 2H, ArHy), 7.03 (d, J=8.1 Hz, 1.7H, ArHx), 7.15 (t, J=8.3 Hz, 1H, ArHy); GC RI: MS m/z (relative intensity, %): 2-allyl-1,3-dimethoxy benzene 5by{1,1} 1378: 179 (M++H, 30), 178 (M+, 100), 1-allyl-2,4-dimethoxy benzene 5bx{1,1} 1411: 179 (M++H, 28), 178 (M+, 100).


5bx,y{1,2-3} Allyl-methyl library B. (Method B, 61% yield): 1H NMR δ: 1.02-1.06 (m, 11.4H, CH3 (Pr)), 1.38-1.42 (m, 7.6H, CH3 (Et)), 1.77-1.84 (m, 7.9H, CH2 (Pr)), 3.30-3.31 (m, 7.1H), 3.42-3.44 (m, 4.4H), 3.80 (m, 10.4H (Mex)), 3.81 (m, 6.1H (Mey)), 3.89-3.93 (m, 7.8H), 4.00-4.05 (m, 4.9H), 4.91-4.93 (m, 2.1H), 4.98-5.04 (m, 8.8H), 5.91-6.01 (m, 5.2H), 6.42-6.46 (m, 7.3H, ArHx), 6.52-6.54 (m, 4.2H, ArHy), 7.00-7.01 (m, 3.2H, ArHx), 7.10-7.13 (m, 2.0H, ArHy); GC RI: MS m/z (relative intensity, %): 2-allyl-1-ethoxy-3-methoxy benzene 5by{1,2} 1435: 193 (M++H, 30), 192 (M+, 70), 163 (M−29, 100); 1-allyl-4-ethoxy-2-methoxy benzene 5bx{1,2} 1480: 193 (M++H, 41), 192 (M+, 100), 163 (M−29, 28); 2-allyl-1-methoxy-3-propoxy benzene 5by{1,3} 1527: 207 (M++H, 62), 206 (M+, 100), 177 (M−29, 68); 1-allyl-2-methoxy-4-propoxy benzene 5bx{1,3} 1573: 207 (M++H, 52), 206 (M+, 100), 177 (M−29, 1).


5bx,y{1,4-5} Allyl-methyl library C. (Method B, 77% yield): 1H NMR δ: 0.95-0.99 (m, 30.7H), 1.45-1.54 (m, 8.7H, CH2 (Bu)), 1.65-1.69 (m, 6.4H), 1.73-1.90 (m, 11.8H), 3.29-3.31 (m, 8.3H), 3.41-3.42 (m, 4.4H), 3.80 (m, 11.7H (Mex)), 3.81 (m, 6.0H (Mey)), 3.93-3.99 (m, 14.4H), 4.90-4.93 (m, 2.1H), 4.96-5.04 (m, 10.1H), 5.90-6.01 (m, 5.6H), 6.41-6.45 (m, 8.6H, ArHx), 6.52-6.54 (m, 4.2H, ArHy), 6.99-7.01 (m, 3.8H, ArHx), 7.10-7.14 (m, 2H, ArHy); GC RI: MS m/z (relative intensity, %): 2-allyl-3-methoxy-1-butoxy benzene 5by{1,4} 1624: 221 (M++H, 36), 220 (M+, 100), 191 (M−29, 79); 1-allyl-2-methoxy-4-butoxy benzene 5bx{1,4} 1672: 221 (M++H, 34), 220 (M+, 100), 191 (M−29, 1); 2-allyl-1-methoxy-3-(3-methyl-butoxy)benzene 5by{1,5} 1680: 235 (M++H, 32), 234 (M+, 100), 205 (M−29, 47); 1-allyl-2-methoxy-4-(3-methyl-butoxy)benzene 5bx{1,5} 1731: 235 (M++H, 31), 234 (M+, 100), 205 (M−29, 0).


5bx,y{2,1} Allyl-ethyl library A (Method B, 70% yield), 5bx,y{2,2-3} allyl-ethyl library B (Method B, 80% yield), 5bx,y{2, 4-5} allyl-ethyl library C (Method B, 48% yield), 5x,y{3,1} allyl-propyl library A (Method B, 88% yield), 5bx,y{3,2-3} allyl-propyl library B (Method B, 80% yield), 5bx,y{3,4-5} allyl-propyl library C (Method B, 62% yield), 5bx,y{4,1} allyl-butyl library A (Method B, 81% yield), 5bx,y{4,2-3} allyl-butyl library B (Method B, 52% yield), 5bx,y{4,4-5} allyl-butyl library C (Method B, 64% yield), 5bx,y{5,1} allyl-ipentyl library A (Method B, 64% yield), 5bx,y{5,2-3} allyl-ipentyl library B (Method B, 74% yield), 5bx,y{5,4-5} allyl-ipentyl library C (Method B, 82% yield), 5bx,y{6,1} allyl-allyl library A (Method B, 67% yield), 5bx,y{6,2-3} allyl-allyl library B (Method B, 53% yield), 5bx,y{6,4-5} allyl-allyl library C (Method B, 76% yield): 1H NMR and GC-MS:


5bx,y{2,1} Allyl-ethyl library A. (Method B, 70% yield): 1H NMR δ: 1.38-1.42 (m, 8.9H, CH3 (Et)), 3.31-3.32 (m, 3.5H, CH2 (Allylx)), 3.42 (dt, J=1.5 and 6.3 Hz, 2H CH2 (Allyly), 3.78 (s, 5.2H (Mex)), 3.82 (s, 3H (Mey)), 3.99-4.05 (m, 6.2H), 4.91-4.94 (m, 1H), 4.98-5.07 (m, 4.6H), 5.91-6.01 (m, 2.5H), 6.42-6.44 (m, 3.4H, ArHx), 6.54 (d, J=8.3 Hz, 2H, ArHy), 7.03 (d, J=7.9 Hz, 1.6H, ArHx), 7.12 (t, J=8.3 Hz, 1H, ArHy); GC RI: MS m/z (relative intensity, %): 2-allyl-1-ethoxy-3-methoxy benzene 5by{2,1} 1435: 193 (M++H, 75), 192 (M+, 100); 1-allyl-2-ethoxy-4-methoxy benzene 5bx{2,1} 1471: 193 (M++H, 47), 192 (M+, 100).


5bx,y{2,2-3} Allyl-ethyl library B. (Method B, 80% yield): 1H NMR δ: 1.01-1.06 (m, 11.6H, CH3 (Pr)), 1.39-1.42 (m, 26.6H, CH3 (Et)), 1.76-1.84 (m, 8H, CH2 (Pr)), 3.31-3.32 (m, 7.7H), 3.42-3.45 (m, 4.4H), 3.88-3.93 (m, 8H, OCH2 (Pr)), 3.98-4.04 (m, 18.1H, OCH2 (Et)), 4.91-4.93 (m, 2.1H), 4.99-5.07 (m, 9.5H), 5.91-6.01 (m, 5.4H), 6.40-6.45 (m, 7.6H, ArHx), 6.50-6.52 (d, 4.1H, ArHy), 7.00-7.02 (m, 3.5H, ArHx), 7.07-7.11 (m, 2H, ArHy); GC RI: MS m/z (relative intensity, %): 2-allyl-1,3-diethoxy benzene 5by{2,2} 1490: 207 (M++H, 80), 206 (M+, 100); 1-allyl-2,4-diethoxy benzene 5bx{2,2} 1535: 207 (M++H, 62), 206 (M+, 100); 2-allyl-1-ethoxy-3-propoxy benzene 5by{2,3} 1587: 221 (M++H, 100), 220 (M+, 94); 1-allyl-2-ethoxy-4-propoxy benzene 5bx{2,3} 1627: 221 (M++H, 67), 220 (M+, 100).


5bx,y{2,4-5} Allyl-ethyl library C. (Method B, 48% yield): 1H NMR δ: 0.95-0.99 (m, 29.6H), 1.38-1.42 (m, 21.5H, CH3 (Et)), 1.45-1.53 (m, 8.5H, CH2CH3 (Bu)), 1.64-1.70 (m, 6.4H, CH2CH (iPent)), 1.72-1.91 (m, 11.2H), 3.30-3.32 (m, 9.1H), 3.42-3.43 (m, 4.3H), 3.92-4.04 (m, 29.2H), 4.90-4.93 (m, 2.1H), 4.98-5.06 (m, 10.7H), 5.90-6.01 (m, 6H), 6.40-6.44 (m, 9H, ArHx), 6.50-6.53 (m, 4.2H, ArHy), 7.00-7.01 (m, 4.2H, ArHx), 7.07-7.11 (m, 2H ArHy); GC RI: MS m/z (relative intensity, %): 2-allyl-1-butoxy-3-ethoxy benzene 5by{2,4} 1682: 235 (M++H, 43), 234 (M+, 85), 149 (M−86, 100); 1-allyl-4-butoxy-2-ethoxy benzene 5bx{2,4} 1724: 235 (M++H, 42), 234 (M+, 100); 2-allyl-1-ethoxy-3-(3-methyl-butoxy) benzene 5by{2,5} 1739: 249 (M++H, 31), 248 (M+, 69), 149 (M−99, 100); 1-allyl-2-ethoxy-4-(3-methyl-butoxy)benzene 5bx{2,5} 1784: 249 (M++H, 34), 248 (M+, 98), 149 (M−99, 100).


5bx,y{3,1} Allyl-propyl library A. (Method B, 88% yield): 1H NMR δ: 1.03-1.06 (m, 9.1H, CH3 (Pr)), 1.77-1.85 (m, 6.4H, CH2CH3 (Pr)), 3.32-3.33 (m, 3.9H, CH2 (Allylx)), 3.43-3.44 (m, 2.2H, CH2 (Allyly), 3.79 (s, 5.6H (Mex)), 3.82 (s, 3H (Mey)), 3.89-3.93 (m, 6.2H, OCH2 (Pr)), 4.91-4.94 (m, 1.1H), 4.98-5.07 (m, 4.7H), 5.91-6.01 (m, 2.8H), 6.41-6.44 (m, 3.8H, ArHx), 6.52-6.54 (m, 2H, ArHy), 7.03 (d, J=8.0 Hz, 1.6H, ArHx), 7.12 (t, J=8.3 Hz, 1H, ArHy); GC RI: MS m/z (relative intensity, %): 2-allyl-1-methoxy-3-propoxy benzene 5by{3,1} 1527: 207 (M++H, 100), 206 (M+, 97); 1-allyl-4-methoxy-2-propoxy benzene 5bx{3,1} 1573: 207 (M++H, 51), 206 (M+, 100).


5bx,y{3,2-3} Allyl-propyl library B. (Method B, 80% yield): 1H NMR δ: 1.03-1.08 (m, 30H, CH3 (Pr)), 1.40-1.43 (m, 7.4H, CH3 (Et)), 1.78-1.86 (m, 20.8H, CH2CH3 (Pr)), 3.33-3.34 (m, 7.5H), 3.45-3.47 (m, 4.4H), 3.90-3.94 (m, 20.4H, OCH2 (Pr)), 4.00-4.06 (m, 5.3H, OCH2 (Et)), 4.92-4.95 (m, 2H), 5.00-5.08 (m, 9H), 5.93-6.03 (m, 4.8H), 6.41-6.46 (m, 7.3H, ArHx), 6.51-6.53 (m, 4.2H, ArHy), 7.01-7.03 (m, 3.4H, ArHx), 7.09-7.12 (m, 2H, ArHy); GC RI: MS m/z (relative intensity, %): 2-allyl-1-ethoxy-3-propoxy benzene 5by{3,2} 1587: 221 (M++H, 39), 220 (M+, 89), 149 (M−71, 100); 1-allyl-4-ethoxy-2-propoxy benzene 5bx{3,2} 1624: 221 (M++H, 29), 220 (M+, 100), 149 (M−71, 53); 2-allyl-1,3-dipropoxy benzene 5by{3,3} 1682: 235 (M++H, 50), 234 (M+, 100); 1-allyl-2,4-dipropoxy benzene 5bx{3,3} 1713: 235 (M++H, 39), 234 (M+, 100).


5bx,y{3,4-5} Allyl-propyl library C. (Method B, 62% yield): 1H NMR δ: 0.95-0.98 (m, 29.4H), 1.02-1.06 (m, 20.9H, CH3 (Pr)), 1.44-1.53 (m, 8.2H, CH2CH3 (Bu)), 1.64-1.69 (m, 6.4H, CH2CH (iPent)), 1.72-1.89 (m, 26.1H), 2.17 (m, 5.8H (Me)), 3.31-3.32 (m, 9H), 3.42-3.44 (m, 4.3H), 3.88-3.98 (m, 29.1H), 4.89-4.92 (m, 2H), 4.98-5.06 (m, 10.6H), 5.89-6.00 (m, 5.8H), 6.39-6.43 (m, 8.9H, ArHx), 6.49-6.52 (m, 4.9H, ArHy), 6.99-7.01 (m, 4.2H, ArHx), 7.07-7.10 (m, 2H, ArHy)); GC RI: MS m/z (relative intensity, %): 2-allyl-1-butoxy-3-propoxy benzene 5by{3,4} 1778: 249 (M++H, 85), 248 (M+, 100); 1-allyl-4-butoxy-2-propoxy benzene 5bx{3,4} 1813: 249 (M++H, 46), 248 (M+, 100); 2-allyl-1-(3-methyl-butoxy)-3-propoxy benzene 5by{3,5} 1835: 263 (M++H, 69), 262 (M+, 100), 1-allyl-2-propoxy-4-(3-methyl-butoxy)benzene 5bx{3,5} 1870: 263 (M++H, 45), 262 (M+, 100).


5bx,y{4,1} Allyl-butyl library A. (Method B, 81% yield): 1H NMR δ: 0.95-0.98 (m, 9.9H, CH3 (Bu)), 1.46-1.54 (m, 6.1H, CH2CH3 (Bu)), 1.73-1.79 (m, 6.3H, OCH2CH2 (Bu)), 3.30-3.32 (m, 3.7H, CH2 (Allylx), 3.42 (dt, J=1.3 and 6.3 Hz, 2H, CH2 (Allyly), 3.78 (s, 5.3H (Mex)), 3.81 (s, 3H (Mey)), 3.92-3.96 (m, 6.3H, OCH2 (Bu)), 4.99-4.93 (m, 1H), 4.96-5.05 (m, 4.7H), 5.90-6.00 (m, 2.7H), 6.40-6.43 (m, 3.6H, ArHx), 6.53 (d, J=8.3 Hz, 2H, ArHy), 7.02 (d, J=8.1 Hz, 1.7H, ArHx), 7.11 (t, J=8.3 Hz, 1H, ArHy); GC RI: MS m/z (relative intensity, %): 2-allyl-1-butoxy-3-methoxy benzene 5by{4,1} 1625: 221 (M++H, 66), 220 (M+, 100); 1-allyl-2-butoxy-4-methoxy benzene 5bx{4,1} 1656: 221 (M++H, 37), 220 (M+, 100).


5bx,y{4,2-3} Allyl-butyl library B. (Method B, 52% yield): 1H NMR δ: 0.95-0.98 (m, 17.7H, CH3 (Bu)), 1.01-1.06 (m, 8.6H, CH3 (Pr)), 1.38-1.41 (m, 9.5H, CH3 (Et)), 1.46-1.54 (m, 12.3H), 1.74-1.83 (m, 18.4H), 3.31-3.32 (m, 7.8H), 3.43-3.45 (m, 3.9H), 3.88-4.04 (m, 25.8H), 4.91-4.93 (m, 1.9H), 4.99-5.06 (m, 9.6H), 5.90-6.01 (m, 5.7H), 6.40-6.45 (m, 8H, ArHx), 6.50-6.52 (m, 4H, ArHy), 7.00-7.01 (m, 3.8H, ArHx), 7.07-7.11 (m, 2H, ArHy); GC RI: MS m/z (relative intensity, %): 2-allyl-1-butoxy-3-ethoxy benzene 5by{4,2} 1681: 235 (M++H, 55), 234 (M+, 88), 149 (M−85, 100); 1-allyl-2-butoxy-4-ethoxy benzene 5bx{4,2} 1714: 235 (M++H, 38), 234 (M+, 100); 2-allyl-1-butoxy-3-propoxy benzene 5by{4,3} 1777: 249 (M++H, 59), 248 (M+, 100); 1-allyl-2-butoxy-4-propoxy benzene 5bx{4,3} 1803: 249 (M++H, 41), 248 (M+, 100).


5bx,y{4,4-5} Allyl-butyl library C. (Method B, 64% yield): 1H NMR δ: 0.95-0.99 (m, 48.9H), 1.43-1.55 (m, 20H, CH2CH3 (Bu)), 1.64-1.70 (m, 8.2H), 1.72-1.90 (m, 25.5H), 3.30-3.32 (m, 7H), 3.42-3.44 (m, 4.1H), 3.92-3.99 (m, 25.5H), 4.90-4.93 (m, 2H), 4.98-5.05 (m, 9.1H), 5.89-6.01 (m, 5.2H), 6.39-6.44 (m, 7.3H, ArHx), 6.50-6.52 (m, 4.1H, ArHy), 6.99-7.01 (m, 3.3H, ArHx), 7.07-7.11 (m, 2H, ArHy); 2-allyl-1,3-dibutoxy benzene 5by{4,4} 1871: 263 (M++H, 72), 262 (M+, 100); 1-allyl-2,4-dibutoxy benzene 5bx{4,4} 1899: 263 (M++H, 41), 262 (M+, 100); 2-allyl-1-butoxy-3-(3-methyl-butoxy)benzene 5by{4,5} 1926: 277 (M++H, 65), 276 (M+, 100); 1-allyl-2-butoxy-4-(3-methyl-butoxy)benzene 5bx{4,5} 1955: 277 (M++H, 42), 276 (M+, 100).


5bx,y{5,1} Allyl-ipentyl library A. (Method B, 64% yield): 1H NMR δ: 0.95-0.97 (m, 16.8H, CH3 (iPent)), 1.66-1.71 (m, 5.8H, CH2CH (iPent)), 1.82-1.91 (m, 3H, CH (iPent)), 3.31-3.32 (m, 3.6H, CH2 (Allylx), 3.41-3.43 (dt, J=1.3 and 6.3 Hz, 2.2H, CH2 (Allyly), 3.79 (s, 5.2H, CH3 (Mex)), 3.81 (s, 3H, CH3 (Mey)), 3.95-3.99 (m, 6H, OCH2 (iPent)), 4.90-4.93 (m, 1H), 4.97-5.06 (m, 4.7H), 5.90-6.00 (m, 2.7H), 6.41-6.45 (m, 3.5H), 6.53-6.55 (m, 2H), 7.03 (d, J=8.2 Hz, 1.7H), 7.12 (t, J=8.3 Hz, 1H); GC RI: MS m/z (relative intensity, %): 2-allyl-1-methoxy-3-(3-methyl-butoxy)benzene 5by{5,1} 1684: 235 (M++H, 43), 234 (M+, 100); 1-allyl-4-methoxy-2-(3-methyl-butoxy)benzene 5bx{5,1} 1711: 235 (M++H, 30), 234 (M+, 100).


5bx,y{5, 2-3} Allyl-ipentyl library B. (Method B, 74% yield): 1H NMR δ: 0.94-0.96 (m, 37.1H, CH3 (iPent)), 1.01-1.05 (m, 9.6H, CH3 (Pr)), 1.38-1.41 (m, 10.4H, CH3 (Et)), 1.65-1.69 (m, 13H), 1.74-1.89 (m, 13.8H), 3.29-3.30 (m, 8.1H), 3.40-3.43 (m, 4.3H), 3.88-4.04 (m, 27H), 4.89-4.92 (m, 2.1H), 4.98-5.05 (m, 10.6H), 5.89-5.99 (m, 6.2H), 6.39-6.44 (m, 8.4H), 6.49-6.52 (m, 4.2H), 6.99-7.00 (m, 4H), 7.07-7.10 (m, 2H): GC RI: MS m/z (relative intensity, %): 2-allyl-1-ethoxy-3-(3-methyl-butoxy)benzene 5by{5,2} 1736: 249 (M++H, 14), 248 (M+, 52), 149 (M−99, 100); 1-allyl-4-ethoxy-2-(3-methyl-butoxy) benzene 5bx{5,2} 1820: 249 (M++H, 22), 248 (M+, 100); 2-allyl-1-(3-methyl-butoxy)-3-propoxy benzene 5by{5,3} 1834: 263 (M++H, 22), 262 (M+, 80), 135 (M−127, 100); 1-allyl-2-(3-methyl-butoxy)-4-propoxy benzene 5bx{5,3} 1855: 263 (M++H, 26), 262 (M+, 100).


5bx,y{5,4-5} Allyl-ipentyl library C. (Method B, 82% yield): 1H NMR δ: 0.96-1.00 (m, 68H), 1.45-1.54 (m, 8.6H, CH2CH3 (Bu)), 1.65-1.92 (m, 40.4H), 3.31-3.32 (m, 6.2H), 3.42-3.44 (m, 4H), 3.93-4.00 (m, 24.7H), 4.90-4.93 (m, 2H), 4.99-5.06 (m, 9.41H), 5.89-6.01 (m, 5.65H), 6.41-6.45 (m, 7.3H), 6.51-6.53 (m, 3.8H), 7.00-7.01 (m, 3.2H), 7.07-7.11 (m, 2H); GC RI: MS m/z (relative intensity, %): 2-allyl-1-butoxy-3-isopentoxy benzene 5by{5,4} 1927: 277 (M++H, 42), 276 (M+, 100); 1-allyl-4-butoxy-2-isopentoxy benzene 5bx{5,4} 1950: 277 (M++H, 32), 276 (M+, 100); 2-allyl-1,3-di(3-methyl-butoxy)benzene 5by{5,5} 1984: 291 (M++H, 36), 290 (M+, 89), 150 (M−140, 100); 1-allyl-2,4-di(3-methyl-butoxy) benzene 5bx {5,5} 2006: 291 (M++H, 32), 290 (M+, 100).


5bx,y{6,1} Allyl-allyl library A. (Method B, 67% yield): 1H NMR δ: 3.35-3.36 (m, 3.8H, CH2 (Allylx), 3.46-3.47 (m, 2H, CH2 (Allyly), 3.79 (s, 5.4H, CH3 (Mex)), 3.83 (s, 3H, CH3 (Mey)), 4.52-4.55 (m, 6.3H), 4.92-4.95 (m, 1.1H), 4.99-5.08 (m, 5H), 5.25-5.30 (m, 3H), 5.41-5.46 (m, 3H), 5.93-6.10 (m, 5.7H), 6.44-6.47 (m, 3.6H), 6.55 (t, J=8.5 Hz, 2H), 7.05 (d, J=8.7 Hz, 1.7H), 7.13 (t, J=8.3 Hz, 1H); GC RI: MS m/z (relative intensity, %): 2-allyl-1-allyloxy-3-methoxy benzene 5by{6,1} 1524: 205 (M++H, 29), 204 (M+, 100); 1-allyl-2-allyloxy-4-methoxy benzene 5bx{6,1} 1554: 205 (M++H, 31), 204 (M+, 100).


5bx,y{6,2-3} Allyl-allyl library B. (Method B, 53% yield): 1H NMR δ: 1.02-1.07 (m, 8.8H, CH3 (Pr)), 1.39-1.42 (m, 9.4H, CH3 (Et)), 1.76-1.85 (m, 6.2H, CH2CH3 (Pr)), 3.34-3.35 (m, 7.7H), 3.46-3.48 (m, 4.3H), 3.88-3.93 (m, 6H, OCH2 (Pr)), 3.98-4.05 (m, 6.5H, OCH2 (Et)), 4.51-4.54 (m, 12.2H), 4.91-4.94 (m, 2H), 5.00-5.07 (m, 10H), 5.24-5.28 (m, 6H), 5.40-5.45 (m, 6H), 5.92-6.09 (m, 12.1H), 6.42-6.45 (m, 7.6H), 6.51-6.54 (m, 4.2H), 7.01-7.03 (m, 3.7H), 7.08-7.11 (m, 2H); GC RI: MS m/z (relative intensity, %): 2-allyl-1-allyloxy-3-ethoxy benzene 5by{6,2} 1581: 219 (M++H, 42), 218 (M+, 100); 1-allyl-2-allyloxy-4-ethoxy benzene 5bx{6,2} 1613: 219 (M++H, 46), 218 (M+, 100); 2-allyl-1-allyloxy-3-propoxy benzene 5by{6,3} 1674: 233 (M++H, 31), 232 (M+, 59), 149 (M−83, 100); 1-allyl-2-allyloxy-4-propoxy benzene 5bx{6,2} 1706: 233 (M++H, 50), 232 (M+, 100).


5bx,y{6,4-5} Allyl-allyl library C. (Method B, 76% yield): 1H NMR δ: 0.96-0.99 (m, 28.1H), 1.45-1.54 (m, 7.5H, CH2CH3 (Bu)), 1.65-1.71 (m, 7H), 1.73-1.92 (m, 10.9H), 3.34-3.36 (m, 6.2H), 3.46-3.47 (m, 3.9H), 3.92-4.00 (m, 12.8H), 4.51-4.55 (m, 10.6H), 4.91-4.94 (m, 2.1H), 5.00-5.07 (m, 9.2H), 5.24-5.29 (m, 5.8H), 5.40-5.45 (m, 5.7H), 5.92-6.10 (m, 11.9H), 6.42-6.45 (m, 6.6H), 6.51-6.55 (m, 4.1H), 7.02-7.04 (m, 3.1H), 7.08-7.12 (m, 2H); GC RI: MS m/z (relative intensity, %): 2-allyl-1-allyloxy-3-butoxy benzene 5by{6,4} 1771: 247 (M++H, 43), 246 (M+, 63), 149 (M−97, 100); 1-allyl-2-allyloxy-4-butoxy benzene 5bx{6,4} 1801: 247 (M++H, 61), 246 (M+, 100); 2-allyl-1-allyloxy-3-(3-methyl-butoxy) benzene 5by{6,5} 1827: 261 (M++H, 74), 260 (M+, 78), 149 (M−111, 100); 1-allyl-2-allyloxy-4-(3-methyl-butoxy)benzene 5bx{6,5} 1861: 261 (M++H, 62), 260 (M+, 100).


5c{1,1-5} Allyl-methyl library. (Method B, 98% yield): 1H NMR δ: 0.95-0.99 (m, 8.6H), 1.03 (t, J=7.5, 3.2H, CH3 (Pr)), 1.38 (t, J=7.0 Hz, 4H), 1.45-1.52 (m, 2H), 1.65 (apparent q, J=6.7 Hz, 2H, CH2 (i-Pent)), 1.71-1.86 (m, 5.2H), 3.35-3.36 (m, 10.8H), 3.76 (s, 4H, OCH3), 3.78-3.79 (m, 16.5H, OCH3), 3.86 (t, J=6.6 Hz, 2.3H, OCH2 (Pr)), 3.89-3.94 (m, 3.6H), 3.97 (q, J=7.0 Hz, 2.4H, OCH2 (Et)), 5.04-5.08 (m, 10H), 5.94-6.02 (m, 4.7H), 6.70-6.80 (m, 15.6H, ArH); GC RI: MS m/z (relative intensity, %): 1-allyl-2,5-dimethoxy benzene 5c{1,1} 1397: 179 (M++H, 28), 178 (M+, 100); 1-allyl-5-ethoxy-2-methoxy benzene 5c{1,2} 1462: 193 (M++H, 32), 192 (M+, 100); 1-allyl-2-methoxy-5-propoxy benzene 5c{1,3} 1557: 207 (M++H, 33), 206 (M+, 100); 1-allyl-5-butoxy-2-methoxy benzene 5c{1,4} 1650: 221 (M++H, 32), 220 (M+, 100); 1-allyl-2-methoxy-5-(3-methyl-butoxy)benzene 5c{1,5} 1709: 235 (M++H, 29), 234 (M+, 100).


5c{2,1-5} Allyl-ethyl library. (Method B, 89% yield), 5c{3,1-5} Allyl-propyl library. (Method B, 95% yield), 5c{4,1-5} Allyl-butyl library. (Method B, 95% yield), 5c{5,1-5} Allyl-iPentyl library. (Method B, 95% yield); 1H NMR and GC-MS data:


5c{2,1-5} Allyl-ethyl library. (Method B, 89% yield): 1H NMR δ: 0.94-0.98 (m, 8.7H), 1.02 (t, J=7.4 Hz, 3.7H), 1.36-1.41 (m, 21H), 1.44-1.52 (m, 2.2H), 1.62-1.66 (m, 4.5H), 1.70-1.86 (m, 5.6H), 3.36-3.38 (m, 10.9H), 3.76 (s, 4H, OCH3), 3.86 (t, J=6.6 Hz, 2.6H), 3.88-3.93 (m, 4H), 3.95-3.99 (m, 14H), 5.03-5.10 (m, 10H), 5.93-6.02 (m, 4.7H), 6.66-6.78 (m, 16.2H, ArH); GC RI: MS m/z (relative intensity, %): 1-allyl-2,5-diethoxy benzene 5c{2,2} 1518: 207 (M++H, 31), 206 (M+, 100); 1-allyl-2-ethoxy-5-propoxy benzene 5c{2,3} 1605: 221 (M++H, 29), 220 (M+, 100); 1-allyl-5-butoxy-2-ethoxy benzene 5c{2,4} 1704: 235 (M++H, 29), 234 (M+, 100); 1-allyl-2-ethoxy-5-(3-methyl-butoxy) benzene 5c{2,5} 1763: 249 (M++H, 27), 248 (M+, 100).


5c{3,1-5} Allyl-propyl library. (Method B, 95% yield): 1H NMR δ: 0.96-1.06 (m, 27.6H), 1.37-1.41 (m, 4H), 1.44-1.53 (m, 2H), 1.64-1.68 (m, 2.9H), 1.72-1.92 (m, 16H), 3.38 (d, J=6.4 Hz, 10.9H), 3.80 (s, 3.8H, OCH3), 3.82-3.99 (m, 19.9H), 4.99-5.18 (m, 10.5H), 5.92-6.05 (m, 5H), 6.67-6.85 (m, 17.5H, ArH); GC RI: MS m/z (relative intensity, %): 1-allyl-2,5-dipropoxy benzene 5c{3,3} 1699: 235 (M++H, 26), 234 (M+, 100); 1-allyl-5-butoxy-2-propoxy benzene 5c{3,4} 1798: 249 (M++H, 27), 248 (M+, 100); 1-allyl-5-(3-methyl-butoxy)-2-propoxy benzene 5c{3,5} 1857: 263 (M++H, 27), 262 (M+, 90), 249 (100).


5c{4,1-5} Allyl-butyl library. (Method B, 95% yield): 1H NMR δ: 0.94-0.98 (m, 19.5H), 1.00-1.04 (m, 3.4H), 1.36-1.39 (m, 3.6H), 1.44-1.54 (m, 9.3H), 1.57-1.58 (m, 2H), 1.64 (t, J=6.8 Hz, 1.8H), 1.70-1.85 (m, 12.5H), 3.35-3.39 (m, 10H), 3.76 (s, 3.7H, OCH3), 3.86 (t, J=6.6 Hz, 2.2H), 3.88-3.93 (m, 11.2H), 3.97 (q, J=6.9 Hz, 2.4H), 5.03-5.17 (m, 9.5H), 5.93-6.06 (m, 4.5H), 6.65-6.87 (m, 16.1H, ArH); GC RI: MS m/z (relative intensity, %): 1-allyl-2,5-dibutoxy benzene 5c{4,4} 1892: 263 (M++H, 28), 262 (M+, 100); 1-allyl-2-butoxy-5-(3-methyl-butoxy)benzene 5c{4,5} 1949: 277 (M++H, 28), 276 (M+, 100).


5c{5,1-5} Allyl-iPentyl library. (Method B, 95% yield): 1H NMR δ: 0.93-0.99 (m, 27.7H), 1.03 (t, J=7.4 Hz, 3.7H), 1.39 (t, J=7.0 Hz, 4H), 1.44-1.52 (m, 2.2H), 1.63-1.88 (m, 18.5H), 3.36-3.39 (m, 10.7H), 3.76 (s, 4H, OCH3), 3.84-3.99 (m, 15.7H), 5.01-5.18 (m, 10.5H), 5.93-6.05 (m, 5H), 6.66-6.85 (m, 17H, ArH); GC RI: MS m/z (relative intensity, %): 1-allyl-2,5-di(3-methyl-butoxy)benzene 5c{5,5} 2001: 291 (M++H, 27), 290 (M+, 100).


The following procedures were used to generate mini-libraries in Set D as set out in Table 2.


The 3c{6,1-5} mini-library (2.7224 g) was heated at 180° C. in a sealed tube, under a nitrogen atmosphere for 30 hours. The viscous dark black oil was purified by column chromatography with chloroform to afford 1.6334 g of pure 6c{1-5} library in 60% yield.


The following data were generated for Set D.



1H NMR δ: 0.92-0.97 (m, 9.5H), 1.02 (t, J=7.4 Hz, 3.6H), 1.37 (t, J=7.0 Hz, 4.2H), 1.45 (d, J=6.2 Hz, 15.7H), 1.57-1.58 (m, 1.4H), 1.64 (q, J=6.8 Hz, 2H), 1.70-1.85 (m, 5.7H), 2.77-2.82 (m, 4.8H), 3.24-3.30 (m, 5H), 3.75 (s, 3.4H, OCH3), 3.85 (t, J=6.6 Hz, 2.2H), 3.87-3.92 (m, 3.9H), 3.96 (q, J=7.0 Hz, 2.3H), 4.85-4.93 (m, 4.2H), 6.63-6.82 (m, 17.6H, ArH); GC RI: MS m/z (relative intensity, %): 5-methoxy-2-methyl-2,3-dihydro benzofuran 6c{1} 1365: 165 (M++H, 24), 164 (M+, 100), 149 (65); 5-ethoxy-2-methyl-2,3-dihydro benzofuran 6c{2} 1434: 179 (M++H, 22), 178 (M+, 100), 149 (25); 5-propoxy-2-methyl-2,3-dihydro benzofuran 6c{3} 1533: 193 (M++H, 22), 192 (M+, 100); 5-butoxy-2-methyl-2,3-dihydro benzofuran 6c{4} 1634: 207 (M++H, 22), 206 (M+, 100); 5-(3-methyl-butoxy)-2-methyl-2,3-dihydro benzofuran 6c{0.5} 1699: 221 (M++H, 22), 220 (M+, 100).


Spectral Data and Analysis of Ethyl, Propyl, Butyl, Isopentyl and Allyl Sets


Data for Compounds in Set A (Dialkoxybenzenes)


Ortho


3a{2,1-5} Ethyl library (Method A, 57% yield): 1H NMR δ: 0.96-0.99 (m, 9.4H), 1.04 (t, J=7.5 Hz, 3H, CH3 (Pr)), 1.41-1.53 (m, 5H), 1.73 (q, J=6.9 Hz, 2H, CH2 (i-Pent)), 1.79-1.89 (m, 5.4H), 3.88 (s, 3H, OCH3 (Me)), 3.97 (t, J=6.8 Hz, 2H), 4.01 (t, J=6.7 Hz, 2H), 4.04 (t, J=6.8 Hz, 2H), 4.05-4.13 (m, 14H), 6.86-6.93 (m, 19H, ArH); GC RI: MS m/z (relative intensity, %): 1,2-diethoxy benzene 3a{2,2} 1244: 167 (M++H, 100), 166 (M+, 81); 1-ethoxy-2-propoxy benzene 3a{2,3} 1335: 181 (M++H, 100), 180 (M+, 60); 1-ethoxy-2-butoxy benzene 3a{2,4} 1429: 195 (M++H, 100), 194 (M+, 83); 1-ethoxy-2-(3-methyl-butoxy)benzene 3a{2,5} 1486: 209 (M++H, 100), 208 (M+, 72).


3a{3,1-5} Propyl library (Method A, 67% yield): 1H NMR δ: 0.96-0.99 (m, 7.4H), 1.04 (t, J=7.4 Hz, 16.5H, CH3 (Pr)), 1.44 (t, J=7.0 Hz, 3H), 1.48-1.53 (m, 1.6H), 1.72 (q, J=6.8 Hz, 1.7H, CH2 (i-Pent)), 1.77-1.91 (m, 14H), 3.87 (s, 3H, OCH3 (Me)), 3.94-4.04 (m, 14.7H), 4.09 (q, J=7.0 Hz, 2H), 6.86-6.92 (m, 17H, Art); GC RI: MS m/z (relative intensity, %): 1,2-dipropoxy benzene 3a{3,3} 1424: 195 (M++H, 100), 194 (M+, 60); 1-butoxy-2-propoxy benzene 3a{3,4} 1518: 209 (M++H, 100), 208 (M+, 84); 1-(3-methyl-butoxy)-2-propoxy benzene 3a{3,5} 1576: 223 (M++H, 100), 222 (M+, 62).


3a{4,1-5} Butyl library (Method A, 62% yield): 1H NMR δ: 0.96-0.99 (m, 24H), 1.04 (t, J=7.5 Hz, 3.4H, CH3 (Pr)), 1.44 (t, J=7.0 Hz, 3H), 1.46-1.54 (m, 12.6H), 1.71 (q, J=6.8 Hz, 2H, CH2 (i-Pent)), 1.77-1.88 (m, 16H), 3.87 (s, 3H, OCH3 (Me)), 3.96 (t, J=6.6 Hz, 2H), 3.98-4.05 (m, 15H), 4.07 (q, J=7.0 Hz, 2.4H), 6.82-6.94 (m, 20H, ArH); GC RI: MS m/z (relative intensity, %): 1,2-dibutoxy benzene 3a{4,4} 1608: 223 (M++H, 100), 222 (M+, 64); 1-butoxy-2-(3-methyl-butoxy)benzene 3a{4,5} 1664: 237 (M++H, 100), 236 (M+, 64).


3a{5,1-5} Isopentyl library. (Method A, 43% yield): 1H NMR δ: 0.96-0.99 (m, 41H), 1.04 (t, J=7.5 Hz, 3.4H, CH3 (Pr)), 1.43 (t, J=7.0 Hz, 3.8H), 1.50 (q, J=7.5 Hz, 2.6H), 1.69-1.90 (m, 24H), 3.86 (s, 3H, OCH3 (Me)), 3.96 (t, J=6.6 Hz, 2H), 3.98-4.10 (m, 18H), 6.84-6.93 (m, 20H, ArH); GC RI: MS m/z (relative intensity, %): 1,2-di(3-methyl-butoxy)benzene 3a{5,5} 1720: 251 (M++H, 20), 250 (M+, 100).


3a{6,1-5} Allyl library. (Method B, 94% yield): 1H NMR δ: 0.96-1.00 (m, 7H), 1.05 (t, J=7.4 Hz, 3H, CH3 (Pr)), 1.45 (t, J=7.0 Hz, 3.7H), 1.49-1.53 (m, 1.7H), 1.73 (q, J=6.9 Hz, 1.4H), 1.79-1.89 (m, 4.6H), 3.88 (s, 4H, OCH3 (Me)), 3.98 (t, J=6.7 Hz, 1.8H), 4.01-4.06 (m, 3.3H), 4.10 (q, J=7.0 Hz, 2.4H), 4.58-4.63 (m, 10.6H), 5.25-5.30 (m, 5.1H), 5.38-5.44 (m, 5H), 6.04-6.14 (m, 5H), 6.84-6.95 (m, 21H, ArH); GC RI: MS m/z (relative intensity, %): 1-allyloxy-2-methoxy benzene 3a{6,1} 1281: 165 (M++H, 42), 164 (M+, 100); 1-allyloxy-2-ethoxy benzene 3a{6,2} 1327: 179 (M++H, 100), 178 (M+, 67); 1-allyloxy-2-propoxy benzene 3a{6,3} 1416: 193 (M++H, 100), 192 (M+, 91); 1-allyloxy-2-butoxy benzene 3a{6,4} 1510: 207 (M++H, 100), 206 (M+, 72); 1-allyloxy-2-(3-methyl-butoxy) benzene 3a{6,5} 1569: 221 (M++H, 100), 220 (M+, 70).


Meta


3b{2,1-5} Ethyl library. (Method A, 66% yield): 1H NMR δ: 0.96 (d, J=6.6 Hz, 6H, CH3 (i-Pent)), 1.03 (t, J=7.4 Hz, 3H, CH3 (Pr)), 1.39-1.43 (m, 12.8H), 1.67 (apparent q, J=6.8 Hz, 2H, CH2 (i-Pent)), 1.78-1.84 (m, 3H), 3.79 (s, 3H, CH3 (Me)), 3.90 (t, J=6.6 Hz, 2H, OCH2 (Pr)), 3.98 (t, J=6.7 Hz, 2H, OCH2 (i-Pent)), 3.99-4.04 (m, 8H), 6.46-6.51 (m, 10H, ArH), 7.16 (t, J=8.2 Hz, 3H, ArH); GC RI: MS m/z (relative intensity, %): 1,3-diethoxy benzene 3b{2,2} 1318: 167 (M++H, 31), 166 (M+, 100); 1-ethoxy-3-propoxy benzene 3b{2,3} 1409: 181 (M++H, 40), 180 (M+, 100); 1-ethoxy-3-(3-methyl-butyloxy)benzene 3b{2,5} 1570: 209 (M++H, 35), 208 (M+, 100).


3b{3,1-5} Propyl library (Method A, 53% yield): 1H NMR δ: 0.96 (d, J=6.6 Hz, 6H, CH3 (i-Pent)), 1.02-1.05 (m, 10H), 1.40 (t, J=7.0 Hz, 2H, CH3 (Et)), 1.67 (apparent q, J=6.8 Hz, 2H, CH2 (i-Pent)), 1.77-1.85 (m, 8H), 3.79 (s, 1.5H, OCH3 (Me)), 3.89-3.92 (m, 7H), 3.97 (t, J=6.7 Hz, 2H, OCH2 (i-Pent)), 4.02 (q, J=7.0 Hz, 1.2H, OCH2 (Et)), 6.46-6.51 (m, 7H, Art), 7.16 (t, J=8.2 Hz, 2.5H, ArH); GC RI: MS m/z (relative intensity, %): 1,3-dipropoxy benzene 3b{3,3} 1501: 195 (M++H, 45), 194 (M+, 100), 110 (85), 82(22); 1-(3-methyl-butyloxy)-3-propoxy benzene 3b{3,5} 1657: 223 (M++H, 44), 222 (M+, 100).


3b{4,1-5} Butyl library (Method A, 69% yield): 1H NMR δ: 0.99-1.02 (m, 19H), 1.05 (t, J=7.0 Hz, 3H, CH3 (Pr)), 1.41-1.45 (m, 3H), 1.48-1.56 (m, 8H), 1.70 (apparent q, J=6.7 Hz, 2.5H, CH2 (i-Pent)), 1.76-1.91 (m, 8H), 3.81 (s, 3H, OCH3 (Me)), 3.93 (t, J=6.6 Hz, 2H, OCH2 (Pr)), 3.95-4.01 (m, 11H), 4.03 (q, J=7.0 Hz, 2H, OCH2 (Et)), 6.45-6.54 (m, 11.7H, Art), 7.16 (t, J=8.2 Hz, 4H, Art); GC RI: MS m/z (relative intensity, %): 1-butoxy-3-methoxy benzene 3b{4,1} 1440: 181 (M++H, 25), 180 (M+, 100); 1-butoxy-3-ethoxy benzene 3b{4,2} 1506: 193 (M++H, 33), 194 (M+, 100); 1-butoxy-3-propoxy benzene 3b{4,3} 1596: 209 (M++H, 48), 208 (M+, 100); 1-butoxy-3-(3-methyl-butyloxy)benzene 3b{4,5} 1754: 237 (M++H, 42), 236 (M+, 100).


3b{5,1-5} Isopentyl library. (Method A, 72% yield): 1H NMR δ: 0.99 (d, J=6.7 Hz, 26H), 1.06 (t, J=7.4 Hz, 3H, CH3 (Pr)), 1.41-1.45 (m, 3H), 1.70 (apparent q, J=6.7 Hz, 9H), 1.81-1.88 (m, 6.3H), 3.81 (s, 3H, OCH3 (Me)), 3.92 (t, J=6.6 Hz, 2H, OCH2 (Pr)), 3.98-4.04 (m, 11H), 6.50-6.54 (m, 11H, ArH), 7.18 (t, J=8.2 Hz, 4H, ArH); GC RI: MS m/z (relative intensity, %): 1-methoxy-3-(3-methyl-butyloxy)benzene 3b{5,1} 1500: 195 (M++H, 26), 194 (M+, 100); 1-ethoxy-3-(3-methyl-butyloxy)benzene 3b{5,2} 1566: 209 (M++H, 35), 208 (M+, 100); 1-(3-methyl-butyloxy)-3-propoxy benzene 3b{5,3} 1653: 223 (M++H, 48), 222 (M+, 100); 1,3-di(3-methyl-butyloxy)benzene 3b{5,5} 1826: 251 (M++H, 40), 250 (M+, 100).


The meta allyl library was synthesized in three portions (methyl by itself, ethyl+propyl and butyl+isopentyl), because upon Claisen rearrangement each compound gave rise to two rearrangement products.


3b {6,1} 1-allyloxy-3-methoxybenzene. (Method D, 98% yield): 1H NMR δ: 3.80 (s, 3H, CH3), 4.53 (apparent d, J=5.5 Hz, 2H, allyl CH2), 5.30 (apparent d, J=14 Hz, 1H), 5.43 (apparent d, J=22 Hz, 1H), 6.07 (m, 1H), 6.52 (m, 3H, ArH), 7.19 (apparent t, J=7.7 Hz, 1H ArH). GC RI: 1334 MS m/z (relative intensity, %): 164 (M+, 100), 149 (M−CH3, 10), 136 (M−28, 12).


3b {6,2-3} Allyl library (ethyl, propyl). (Method D, 60% yield, 35% 3b {6,2} by GC and 39% by 1H NMR and the rest is 3b {6,3}): 1H NMR δ: 1.04 (t, J=4 Hz, 3H, CH3 propyl), 1.42 (t, J=3.7 Hz, 3H, CH3 ethyl), 1.81 (m, 2H, CH2, propyl), 3.95 (t, J=3.7 Hz, 2H propyl CH2), 4.02 (q, J=7 Hz, 2H, ethyl), 4.53 (apparent d, J=7 Hz, 2H for each component), 5.29 (m, J=14 Hz, 1H for each component), 5.41 (m, J=22 Hz, 1H for each component), 6.07 (m, 1H for each component), 6.53 (m, 3H for each component), 7.17 (apparent t, J=8 Hz, 1H for each component). GC RI: MS m/z (relative intensity, %): 1-allyloxy-3-ethoxybenzene 3b {6,2} 1398: 179 (M+1, 72), 178 (M+, 100), 150 (M−28, 35); 1-allyloxy-3-propoxybenzene 3b {6,3} 1491: 193 (M+1, 93), 192 (M+, 100), 164 (M−28, 12), 150 (31).


3b {6,4-5} Allyl library (butyl, isopentyl). (Method D, 71% yield, 3b {6,4} 34% by GC and 40% by 1H NMR and the rest is 3b {6,5}): 1H NMR δ: 0.98 (m, 6H, CH3 isopentyl, 3H CH3 butyl), 1.48 (m, 2H, CH2 butyl), 1.68 (m, 2H, CH2, isopentyl), 1.75 (m, 2H, CH2, butyl), 1.83 (m, 1H, isopentyl), 3.96 (m, 2H for each component, CH2), 4.52 (apparent d, J=8 Hz, 2H for each component), 5.29 (apparent d, J=14 Hz, 1H for each component), 5.42 (apparent d, J=22 Hz, 1H for each component), 6.06 (m, 1H for each component), 6.51 (m, 3H for each component, ArH), 7.17 (apparent t, J=7 Hz, 1H for each component, ArH). GC RI: MS m/z (relative intensity, %): 1-allyloxy-3-n-butoxybenzene 3b {6,4} 1592: 207 (M+1, 83), 206 (M+, 100), 178 (M−28, 12), 150 (33). 1-allyloxy-3-isopentyloxybenzene 3b {6,5} 1654: 221 (M+1, 81), 220 (M+, 100), 192 (M−28, 7), 150 (21).


Para


3c{2,1-5} Ethyl library (Method A, 31% yield): 1H NMR δ. 0.96 (d, J=6.7 Hz, 6H, CH3 (i-Pent)), 1.03 (t, J=7.4 Hz, 3H, CH3 (Pr)), 1.39 (t, J=7.0 Hz, 15H, CH3 (Et)), 1.66 (apparent q, J=6.8 Hz, 3H, CH2 (i-Pent)), 1.77-1.83 (m, 4H), 3.77 (s, 3H, OCH3 (Me)), 3.87 (t, J=6.6 Hz, 2H, OCH2 (Pr)), 3.94 (t, J=6.7 Hz, 2H, OCH2 (i-Pent)), 3.98 (q, J=7.0 Hz, 10H, OCH2 (Et)), 6.83-6.84 (m, 16H, ArH); GC RI: MS m/z (relative intensity, %): 1,4-diethoxy benzene 3c{2,2} 1248: 167 (M++H, 33), 166 (M+, 100); 1-ethoxy-4-propoxy benzene 3c{2,3} 1337: 181 (M++H, 28), 180 (M+, 100); 1-ethoxy-4-(3-methyl-butyloxy) benzene 3c{2,5} 1492: 209 (M++H, 31), 208 (M+, 100).


3c{3,1-5} Propyl library (Method A, 82% yield): 1H NMR δ: 0.97 (d, J=6.6 Hz, 7H, CH3 (i-Pent)), 1.03 (t, J=7.4 Hz, 15H, CH3 (Pr)), 1.40 (t, J=6.9 Hz, 3H, CH3 (Et)), 1.66 (apparent q, J=6.8 Hz, 3H, CH2 (i-Pent)), 1.77-1.85 (m, 12H), 3.77 (s, 3H, OCH3 (Me)), 3.87 (t, J=6.5 Hz, 10H, OCH2 (Pr)), 3.94 (t, J=6.6 Hz, 2.8H, OCH2 (i-Pent)), 3.98 (q, J=7.1 Hz, 2H, OCH2 (Et)), 6.83-6.84 (m, 16H, ArH); GC RI: MS m/z (relative intensity, %): 1,4-dipropoxy benzene 3c{3,3} 1431: (M++H, 25), 194 (M+, 100); 1-(3-methyl-butyloxy)-4-propoxy benzene 3c{3,5} 1589: 223 (M++H, 28), 222 (M+, 100).


3c{4,1-5} Butyl library (Method A, 76% yield): 1H NMR δ. 0.96-0.99 (m, 18H), 1.02 (t, J=7.4 Hz, 3H, CH3 (Pr)), 1.39 (t, J=6.9 Hz, 4H, CH3 (Et)), 1.45-1.53 (m, 8H, CH2 (Bu)), 1.66 (apparent q, J=6.7 Hz, 2H, CH2 (i-Pent)), 1.72-1.81 (m, 11.6H), 3.77 (s, 3H, OCH3 (Me)), 3.87 (t, J=6.6 Hz, 2H, OCH2 (Pr)), 3.90-3.95 (m, 10.4H), 3.98 (q, J=7.0 Hz, 2H, OCH2 (Et)), 6.83-6.84 (m, 16H, ArH); GC RI: MS m/z (relative intensity, %): 1-butoxy-4-methoxy benzene 3c{4,1} 1371: 181 (M++H, 29), 180 (M+, 100); 1-butoxy-4-ethoxy benzene 3c{4,2} 1437: 195 (M++H, 23), 194 (M+, 100); 1-butoxy-4-propoxy benzene 3c{4,3} 1529: 209 (M++H, 40), 208 (M+, 100); 1-butoxy-4-(3-methyl-butyloxy)benzene 3c{4,5} 1681: 237 (M++H, 42), 236 (M+, 100).


3c{5,1-5} Isopentyl (3-methyl-butyloxy) library. (Method A, 82% yield): 1H NMR δ. 0.96 (d, J=7.0 Hz 30H, CH3 (i-Pent)), 1.02 (t, J=7.4 Hz, 3H, CH3 (Pr)), 1.39 (t, J=6.9 Hz, 3H, CH3 (Et)), 1.66 (apparent q, J=6.8 Hz, 10H, CH2 (i-Pent)), 1.75-1.86 (m, 7.5H), 3.77 (s, 3H, OCH3 (Me)), 3.87 (t, J=6.4 Hz, 2H, OCH2 (Pr)), 3.94 (t, J=6.9 Hz, 10H, OCH2 (i-Pent)), 3.98 (q, J=6.8 Hz, 2H, OCH2 (Et)), 6.83-6.84 (m, 16H, ArH); GC RI: MS m/z (relative intensity, %): 1,4-di(3-methyl-butyloxy)-benzene 3c{5,5} 1850: 251 (M++H, 25), 250 (M+, 100).


3c{6,1-5} Allyl library. (Method B, 95% yield): GC (RI): 1H NMR δ: 0.95-0.98 (m, 8H), 1.03 (t, J=7.4 Hz, 3H, CH3 (Pr)), 1.39 (t, J=7.0 Hz, 3.9H, CH3 (Et)), 1.46-1.50 (m, 1.5H), 1.56 (d, J=3.8 Hz, 1.3H), 1.65 (apparent q, J=6.8 Hz, 2H, CH2 (i-Pent)), 1.71-1.85 (m, 5H), 3.78 (s, 4H, OCH3 (Me)), 3.87 (t, J=6.6 Hz, 2H, OCH2 (Pr)), 3.90-3.95 (m, 3.7H), 3.98 (q, J=7.0 Hz, 2.5H, OCH2 (Et)), 4.47-4.49 (m, 10.9H), 5.25-5.29 (m, 5.H), 5.38-5.42 (m, 5H), 6.01-6.09 (m, 5H), 6.81-6.87 (m, 21H, ArH); GC RI: MS m/z (relative intensity, %): 1-allyloxy-4-methoxy benzene 3c{6,1} 1326: 165 (M++H, 20), 164 (M+, 100); 1-allyloxy-4-ethoxy benzene 3c{6,2} 1394: 179 (M++H, 70), 178 (M+, 100); 1-allyloxy-4-propoxy benzene 3c{6,3} 1491: 193 (M++H, 65), 192 (M+, 100); 1-allyloxy-4-butoxy benzene 3c{6,4} 1594: 207 (M++H, 56), 206 (M+, 100); 1-allyloxy-4-(3-methyl-butoxy)benzene 3c{6,5} 1659: 221 (M++H, 46), 220 (M+, 100).


Data for Compounds in Set C (Allyl Dialkoxybenzenes)


Ortho


5a{2,1-5} Allyl-ethyl library. (Method B, 91% yield): 1H NMR δ: 0.97-1.00 (m, 11.5H), 1.35-1.40 (m, 14.8H), 1.42-1.16 (m, 10.5H), 1.72 (apparent q, J=6.7 Hz, 1.8H, CH2 (i-Pent)), 1.78-1.91 (m, 5.7H), 3.43 (d, J=6.6 Hz, 9.2H), 3.84 (s, 3.9H, OCH3), 3.91-4.12 (m, 20.9H), 5.01-5.10 (m, 10.3H), 5.94-6.02 (m, 5H), 6.69-6.83 (m, 11.4H, ArH), 6.90-7.00 (m, 6.7H, ArH); GC RI: MS m/z (relative intensity, %): 1-allyl-2,3-diethoxy benzene 5a{2,2} 1435: 207 (M++H, 63), 206 (M+, 100); 1-allyl-2-ethoxy-3-propoxy benzene 5a{2,3} 1523: 221 (M++H, 56), 220 (M+, 100); 1-allyl-2-butoxy-3-ethoxy benzene 5a{2,4} 1616: 235 (M++H, 88), 234 (M+, 100); 1-allyl-2-ethoxy-3-(3-methyl-butoxy)benzene 5a{2,5} 1669: 249 (M++H, 79), 248 (M+, 100).


5a{3,1-5} Allyl-propyl library. (Method B, 96% yield): 1H NMR δ: 0.97-1.08 (m, 27.8H), 1.44 (t, J=7.0 Hz, 4H), 1.49-1.56 (m, 2.3H), 1.69-1.89 (m, 16.3H), 3.42 (d, J=6.6 Hz, 9.5H), 3.84 (s, 4H, OCH3), 3.86-4.09 (m, 21H), 5.02-5.08 (m, 10H), 5.94-6.02 (m, 5H), 6.69-6.83 (m, 11.4H, ArH), 6.89-6.99 (m, 6H, ArH); GC RI: MS m/z (relative intensity, %): 1-allyl-2,3-dipropoxy benzene 5a{3,3} 1608: 235 (M++H, 57), 234 (M+, 100); 1-allyl-3-butoxy-2-propoxy benzene 5a{3,4} 1699: 249 (M++H, 100), 248 (M+, 72); 1-allyl-3-(3-methyl-butoxy)-2-propoxy benzene 5a{3,5} 1751: 263 (M++H, 50), 262 (M+, 90), 249 (100).


5a{4,1-5} Allyl-butyl library. (Method B, 92% yield): 1H NMR δ: 0.96-0.99 (m, 22.3H), 1.05 (t, J=7.4 Hz, 2.7H), 1.43 (t, J=6.9 Hz, 4.2H), 1.47-1.54 (m, 12.2H), 1.69-1.89 (m, 16.7H), 3.42 (d, J=6.6 Hz, 9.2H), 3.84 (s, 4H, OCH3), 3.88-4.11 (m, 19H), 5.02-5.10 (m, 10.3H), 5.93-6.01 (m, 5H), 6.69-6.83 (m, 11.4H, ArH), 6.89-6.99 (m, 6.5H, ArH); GC RI: MS m/z (relative intensity, %): 1-allyl-2,3-dibutoxy benzene 5a{4,4} 1784: 263 (M++H, 27), 262 (M+, 100); 1-allyl-2-butoxy-3-(3-methyl-butoxy)benzene 5a{4,5} 1833: 277 (M++H, 25), 276 (M+, 100).


5a{5,1-5} Allyl-iPentyl library. (Method B, 90% yield): 1H NMR δ: 0.95-1.00 (m, 37.6H), 1.06 (t, J=7.5 Hz, 2.7H), 1.44 (t, J=7.0 Hz, 4.3H), 1.49-1.55 (m, 2.1H), 1.65-1.72 (m, 12.4H), 1.78-1.90 (m, 10H), 3.41 (d, J=6.6 Hz, 9.4H), 3.84 (s, 4H, OCH3), 3.91-4.08 (m, 19H), 5.01-5.09 (m, 10.2H), 5.93-6.01 (m, 5H), 6.69-6.83 (m, 11.1H, ArH), 6.89-6.99 (m, 7.2H, ArH); GC RI: MS m/z (relative intensity, %): 1-allyl-2,3-di(3-methyl-butoxy)benzene 5a{5,5} 1879: 291 (M++H, 23), 290 (M+, 100).


5a{6,1-5} Allyl-allyl library. (Method B, 90% yield): 1H NMR δ: 0.88-0.93 (m, 11.3H), 0.96-1.01 (m, 2.7H), 1.36-1.39 (m, 2.7H), 1.45 (t, J=7.3 Hz, 2.7H), 1.65 (q, J=6.7 Hz, 2.3H), 1.70-1.83 (m, 6H), 3.25 (d, J=7.0 Hz, 1.5H), 3.35 (d, J=6.6 Hz, 8.7H), 3.78 (s, 2.5H, OCH3), 3.86-4.04 (m, 9.5H), 4.40-4.54 (m, 10.3H), 4.95-5.02 (m, 10.2H), 5.13-5.20 (m, 5H), 5.27-5.36 (m, 5H), 5.84-5.92 (m, 5H), 5.97-6.08 (m, 5H), 6.61-6.76 (m, 11.1H, ArH), 6.82-6.94 (m, 5H, ArH); GC RI: MS m/z (relative intensity, %): 1-allyl-2-allyloxy-3-methoxy benzene 5a{6,1} 1463: 205 (M++H, 84), 204 (M+, 100); 1-allyl-2-allyloxy-3-ethoxy benzene 5a{6,2} 1509: 219 (M++H, 100), 218 (M+, 95); 1-allyl-2-allyloxy-3-propoxy benzene 5a{6,3} 1597: 233 (M++H, 100), 232 (M+, 87); 1-allyl-2-allyloxy-3-butoxy benzene 5a{6,4} 1688: 247 (M++H, 100), 246 (M+, 91); 1-allyl-2-allyloxy-3-(3-methyl-butoxy)benzene 5a{6,5} 1740: 261 (M++H, 100), 260 (M+, 92).


Meta


5bx,y{2,1} Allyl-ethyl library A. (Method B, 70% yield): 1H NMR δ: 1.38-1.42 (m, 8.9H, CH3 (Et)), 3.31-3.32 (m, 3.5H, CH2(Allylx)), 3.42 (dt, J=1.5 and 6.3 Hz, 2H CH2 (Allyly)), 3.78 (s, 5.2H (Mex)), 3.82 (s, 3H (Mey)), 3.99-4.05 (m, 6.2H), 4.91-4.94 (m, 1H), 4.98-5.07 (m, 4.6H), 5.91-6.01 (m, 2.5H), 6.42-6.44 (m, 3.4H, ArHx), 6.54 (d, J=8.3 Hz, 2H, ArHy), 7.03 (d, J=7.9 Hz, 1.6H, ArHx), 7.12 (t, J=8.3 Hz, 1H, ArHy); GC RI: MS m/z (relative intensity, %): 2-allyl-1-ethoxy-3-methoxy benzene 5by{2,1} 1435: 193 (M++H, 75), 192 (M+, 100); 1-allyl-2-ethoxy-4-methoxy benzene 5bx{2,1} 1471: 193 (M++H, 47), 192 (M+, 100).


5bx,y{2,2-3} Allyl-ethyl library B. (Method B, 80% yield): 1H NMR δ: 1.01-1.06 (m, 11.6H, CH3 (Pr)), 1.39-1.42 (m, 26.6H, CH3 (Et)), 1.76-1.84 (m, 8H, CH2 (Pr)), 3.31-3.32 (m, 7.7H), 3.42-3.45 (m, 4.4H), 3.88-3.93 (m, 8H, OCH2 (Pr)), 3.98-4.04 (m, 18.1H, OCH2 (Et)), 4.91-4.93 (m, 2.1H), 4.99-5.07 (m, 9.5H), 5.91-6.01 (m, 5.4H), 6.40-6.45 (m, 7.6H, ArHx), 6.50-6.52 (d, 4.1H, ArHy), 7.00-7.02 (m, 3.5H, ArHx), 7.07-7.11 (m, 2H, ArHy); GC RI: MS m/z (relative intensity, %): 2-allyl-1,3-diethoxy benzene 5by{2,2} 1490: 207 (M++H, 80), 206 (M+, 100); 1-allyl-2,4-diethoxy benzene 5bx{2,2} 1535: 207 (M++H, 62), 206 (M+, 100); 2-allyl-1-ethoxy-3-propoxy benzene 5by{2,3} 1587: 221 (M++H, 100), 220 (M+, 94); 1-allyl-2-ethoxy-4-propoxy benzene 5bx{2,3} 1627: 221 (M++H, 67), 220 (M+, 100).


5bx,y{2,4-5} Allyl-ethyl library C. (Method B, 48% yield): 1H NMR δ: 0.95-0.99 (m, 29.6H), 1.38-1.42 (m, 21.5H, CH3 (Et)), 1.45-1.53 (m, 8.5H, CH2CH3 (Bu)), 1.64-1.70 (m, 6.4H, CH2CH (iPent)), 1.72-1.91 (m, 11.2H), 3.30-3.32 (m, 9.1H), 3.42-3.43 (m, 4.3H), 3.92-4.04 (m, 29.2H), 4.90-4.93 (m, 2.1H), 4.98-5.06 (m, 10.7H), 5.90-6.01 (m, 6H), 6.40-6.44 (m, 9H, ArHx), 6.50-6.53 (m, 4.2H, ArHy), 7.00-7.01 (m, 4.2H, ArHx), 7.07-7.11 (m, 2H ArHy); GC RI: MS m/z (relative intensity, %): 2-allyl-1-butoxy-3-ethoxy benzene 5by{2,4} 1682: 235 (M++H, 43), 234 (M+, 85), 149 (M−86, 100); 1-allyl-4-butoxy-2-ethoxy benzene 5bx{2,4} 1724: 235 (M++H, 42), 234 (M+, 100); 2-allyl-1-ethoxy-3-(3-methyl-butoxy)benzene 5by{2,5} 1739: 249 (M++H, 31), 248 (M+, 69), 149 (M−99, 100); 1-allyl-2-ethoxy-4-(3-methyl-butoxy)benzene 5bx{2,5} 1784: 249 (M++H, 34), 248 (Mt 98), 149 (M−99, 100).


5bx,y{3,1} Allyl-propyl library A. (Method B, 88% yield): 1H NMR δ: 1.03-1.06 (m, 9.1H, CH3 (Pr)), 1.77-1.85 (m, 6.4H, CH2CH3 (Pr)), 3.32-3.33 (m, 3.9H, CH2 (Allylx)), 3.43-3.44 (m, 2.2H, CH2 (Allyly), 3.79 (s, 5.6H (Mex)), 3.82 (s, 3H (Mey)), 3.89-3.93 (m, 6.2H, OCH2 (Pr)), 4.91-4.94 (m, 1.1H), 4.98-5.07 (m, 4.7H), 5.91-6.01 (m, 2.8H), 6.41-6.44 (m, 3.8H, ArHx), 6.52-6.54 (m, 2H, ArHy), 7.03 (d, J=8.0 Hz, 1.6H, ArHx), 7.12 (t, J=8.3 Hz, 1H, ArHy); GC RI: MS m/z (relative intensity, %): 2-allyl-1-methoxy-3-propoxy benzene 5by{3,1} 1527: 207 (M++H, 100), 206 (M+, 97); 1-allyl-4-methoxy-2-propoxy benzene 5bx {3,1} 1573: 207 (M++H, 51), 206 (M+, 100).


5bx,y{3,2-3} Allyl-propyl library B. (Method B, 80% yield): 1H NMR δ: 1.03-1.08 (m, 30H, CH3 (Pr)), 1.40-1.43 (m, 7.4H, CH3 (Et)), 1.78-1.86 (m, 20.8H, CH2CH3 (Pr)), 3.33-3.34 (m, 7.5H), 3.45-3.47 (m, 4.4H), 3.90-3.94 (m, 20.4H, OCH2 (Pr)), 4.00-4.06 (m, 5.3H, OCH2 (Et)), 4.92-4.95 (m, 2H), 5.00-5.08 (m, 9H), 5.93-6.03 (m, 4.8H), 6.41-6.46 (m, 7.3H, ArHx), 6.51-6.53 (m, 4.2H, ArHy), 7.01-7.03 (m, 3.4H, ArHx), 7.09-7.12 (m, 2H, ArHy); GC RI: MS m/z (relative intensity, %): 2-allyl-1-ethoxy-3-propoxy benzene 5by{3,2} 1587: 221 (M++H, 39), 220 (M+, 89), 149 (M−71, 100); 1-allyl-4-ethoxy-2-propoxy benzene 5bx{3,2} 1624: 221 (M++H, 29), 220 (M+, 100), 149 (M−71, 53); 2-allyl-1,3-dipropoxy benzene 5by{3,3} 1682: 235 (M++H, 50), 234 (M+, 100); 1-allyl-2,4-dipropoxy benzene 5bx{3,3} 1713: 235 (M++H, 39), 234 (M+, 100).


5bx,y{3,4-5} Allyl-propyl library C. (Method B, 62% yield): 1H NMR δ: 0.95-0.98 (m, 29.4H), 1.02-1.06 (m, 20.9H, CH3 (Pr)), 1.44-1.53 (m, 8.2H, CH2CH3 (Bu)), 1.64-1.69 (m, 6.4H, CH2CH (iPent)), 1.72-1.89 (m, 26.1H), 2.17 (m, 5.8H (Me)), 3.31-3.32 (m, 9H), 3.42-3.44 (m, 4.3H), 3.88-3.98 (m, 29.1H), 4.89-4.92 (m, 2H), 4.98-5.06 (m, 10.6H), 5.89-6.00 (m, 5.8H), 6.39-6.43 (m, 8.9H, ArHx), 6.49-6.52 (m, 4.9H, ArHy), 6.99-7.01 (m, 4.2H, ArHx), 7.07-7.10 (m, 2H, ArHy); GC RI: MS m/z (relative intensity, %): 2-allyl-1-butoxy-3-propoxy benzene 5by{3,4} 1778: 249 (M++H, 85), 248 (M+, 100); 1-allyl-4-butoxy-2-propoxy benzene 5bx{3,4} 1813: 249 (M++H, 46), 248 (M+, 100); 2-allyl-1-(3-methyl-butoxy)-3-propoxy benzene 5by{3,5} 1835: 263 (M++H, 69), 262 (M+, 100), 1-allyl-2-propoxy-4-(3-methyl-butoxy)benzene 5bx{3,5} 1870: 263 (M++H, 45), 262 (M+, 100).


5bx,y{4,1} Allyl-butyl library A. (Method B, 81% yield): 1H NMR δ: 0.95-0.98 (m, 9.9H, CH3 (Bu)), 1.46-1.54 (m, 6.1H, CH2CH3 (Bu)), 1.73-1.79 (m, 6.3H, OCH2CH2 (Bu)), 3.30-3.32 (m, 3.7H, CH2 (Allylx), 3.42 (dt, J=1.3 and 6.3 Hz, 2H, CH2 (Allyly), 3.78 (s, 5.3H (Mex)), 3.81 (s, 3H (Mey)), 3.92-3.96 (m, 6.3H, OCH2 (Bu)), 4.99-4.93 (m, 1H), 4.96-5.05 (m, 4.7H), 5.90-6.00 (m, 2.7H), 6.40-6.43 (m, 3.6H, ArHx), 6.53 (d, J=8.3 Hz, 2H, ArHy), 7.02 (d, J=8.1 Hz, 1.7H, ArHx), 7.11 (t, J=8.3 Hz, 1H, ArHy); GC RI: MS m/z (relative intensity, %): 2-allyl-1-butoxy-3-methoxy benzene 5by{4,1} 1625: 221 (M++H, 66), 220 (M+, 100); 1-allyl-2-butoxy-4-methoxy benzene 5bx{4,1} 1656: 221 (M++H, 37), 220 (M+, 100).


5bx,y{4,2-3} Allyl-butyl library B. (Method B, 52% yield): 1H NMR δ: 0.95-0.98 (m, 17.7H, CH3 (Bu)), 1.01-1.06 (m, 8.6H, CH3 (Pr)), 1.38-1.41 (m, 9.5H, CH3 (Et)), 1.46-1.54 (m, 12.3H), 1.74-1.83 (m, 18.4H), 3.31-3.32 (m, 7.8H), 3.43-3.45 (m, 3.9H), 3.88-4.04 (m, 25.8H), 4.91-4.93 (m, 10.9H), 4.99-5.06 (m, 9.6H), 5.90-6.01 (m, 5.7H), 6.40-6.45 (m, 8H, ArHx), 6.50-6.52 (m, 4H, ArHy), 7.00-7.01 (m, 3.81-1, ArHx), 7.07-7.11 (m, 2H, ArHy); GC RI: MS m/z (relative intensity, %): 2-allyl-1-butoxy-3-ethoxy benzene 5by{4,2} 1681: 235 (M++H, 55), 234 (M+, 88), 149 (M−85, 100); 1-allyl-2-butoxy-4-ethoxy benzene 5bx{4,2} 1714: 235 (M++H, 38), 234 (M+, 100); 2-allyl-1-butoxy-3-propoxy benzene 5by{4,3} 1777: 249 (M++H, 59), 248 (M+, 100); 1-allyl-2-butoxy-4-propoxy benzene 5bx{4,3} 1803: 249 (M++H, 41), 248 (M+, 100).


5bx,y{4,4-5} Allyl-butyl library C. (Method B, 64% yield): 1H NMR δ: 0.95-0.99 (m, 48.9H), 1.43-1.55 (m, 20H, CH2CH3 (Bu)), 1.64-1.70 (m, 8.2H), 1.72-1.90 (m, 25.5H), 3.30-3.32 (m, 7H), 3.42-3.44 (m, 4.1H), 3.92-3.99 (m, 25.5H), 4.90-4.93 (m, 2H), 4.98-5.05 (m, 9.1H), 5.89-6.01 (m, 5.2H), 6.39-6.44 (m, 7.3H, ArHx), 6.50-6.52 (m, 4.1H, ArHy), 6.99-7.01 (m, 3.3H, ArHx), 7.07-7.11 (m, 2H, ArHy); 2-allyl-1,3-dibutoxy benzene 5by{4,4} 1871: 263 (M++H, 72), 262 (M+, 100); 1-allyl-2,4-dibutoxy benzene 5bx{4,4} 1899: 263 (M++H, 41), 262 (M+, 100); 2-allyl-1-butoxy-3-(3-methyl-butoxy)benzene 5by{4,5} 1926: 277 (M++H, 65), 276 (M+, 100); 1-allyl-2-butoxy-4-(3-methyl-butoxy)benzene 5bx{4,5} 1955: 277 (M++H, 42), 276 (M+, 100).


5bx,y{5,1} Allyl-ipentyl library A. (Method B, 64% yield): 1H NMR δ: 0.95-0.97 (m, 16.8H, CH3 (iPent)), 1.66-1.71 (m, 5.8H, CH2CH (iPent)), 1.82-1.91 (m, 3H, CH (iPent)), 3.31-3.32 (m, 3.6H, CH2 (Allylx), 3.41-3.43 (dt, J=1.3 and 6.3 Hz, 2.2H, CH2 (Allyly), 3.79 (s, 5.2H, CH3 (Mex)), 3.81 (s, 3H, CH3 (Mey)), 3.95-3.99 (m, 6H, OCH2 (iPent)), 4.90-4.93 (m, 1H), 4.97-5.06 (m, 4.7H), 5.90-6.00 (m, 2.7H), 6.41-6.45 (m, 3.5H), 6.53-6.55 (m, 2H), 7.03 (d, J=8.2 Hz, 1.7H), 7.12 (t, J=8.3 Hz, 1H); GC RI: MS m/z (relative intensity, %): 2-allyl-1-methoxy-3-(3-methyl-butoxy)benzene 5by{5,1} 1684: 235 (M++H, 43), 234 (M+, 100); 1-allyl-4-methoxy-2-(3-methyl-butoxy)benzene 5bx{5,1} 1711: 235 (M++H, 30), 234 (M+, 100).


5bx,y{5,2-3} Allyl-ipentyl library B. (Method B, 74% yield): 1H NMR δ: 0.94-0.96 (m, 37.1H, CH3 (iPent)), 1.01-1.05 (m, 9.6H, CH3 (Pr)), 1.38-1.41 (m, 10.4H, CH3 (Et)), 1.65-1.69 (m, 13H), 1.74-1.89 (m, 13.8H), 3.29-3.30 (m, 8.1H), 3.40-3.43 (m, 4.3H), 3.88-4.04 (m, 27H), 4.89-4.92 (m, 2.1H), 4.98-5.05 (m, 10.6H), 5.89-5.99 (m, 6.2H), 6.39-6.44 (m, 8.4H), 6.49-6.52 (m, 4.2H), 6.99-7.00 (m, 4H), 7.07-7.10 (m, 2H): GC RI: MS m/z (relative intensity, %): 2-allyl-1-ethoxy-3-(3-methyl-butoxy)benzene 5by{5,2} 1736: 249 (M++H, 14), 248 (M+, 52), 149 (M−99, 100); 1-allyl-4-ethoxy-2-(3-methyl-butoxy)benzene 5bx{5,2} 1820: 249 (M++H, 22), 248 (M+, 100); 2-allyl-1-(3-methyl-butoxy)-3-propoxy benzene 5by{5,3} 1834: 263 (M++H, 22), 262 (M+, 80), 135 (M−127, 100); 1-allyl-2-(3-methyl-butoxy)-4-propoxy benzene 5bx{5,3} 1855: 263 (M++H, 26), 262 (M+, 100).


5bx,y{5,4-5} Allyl-ipentyl library C. (Method B, 82% yield): 1H NMR δ: 0.96-1.00 (m, 68H), 1.45-1.54 (m, 8.6H, CH2CH3 (Bu)), 1.65-1.92 (m, 40.4H), 3.31-3.32 (m, 6.2H), 3.42-3.44 (m, 4H), 3.93-4.00 (m, 24.7H), 4.90-4.93 (m, 2H), 4.99-5.06 (m, 9.41H), 5.89-6.01 (m, 5.65H), 6.41-6.45 (m, 7.3H), 6.51-6.53 (m, 3.8H), 7.00-7.01 (m, 3.2H), 7.07-7.11 (m, 2H); GC RI: MS m/z (relative intensity, %): 2-allyl-1-butoxy-3-isopentoxy benzene 5by{5,4} 1927: 277 (M++H, 42), 276 (M+, 100); 1-allyl-4-butoxy-2-isopentoxy benzene 5bx{5,4} 1950: 277 (M++H, 32), 276 (M+, 100); 2-allyl-1,3-di(3-methyl-butoxy)benzene 5by{5,5} 1984: 291 (M++H, 36), 290 (M+, 89), 150 (M−140, 100); 1-allyl-2,4-di(3-methyl-butoxy) benzene 5bx{5,5} 2006: 291 (M++H, 32), 290 (M+, 100).


5bx,y{6,1} Allyl-allyl library A. (Method B, 67% yield): 1H NMR δ: 3.35-3.36 (m, 3.8H, CH2 (Allylx), 3.46-3.47 (m, 2H, CH2 (Allyly), 3.79 (s, 5.4H, CH3 (Mex)), 3.83 (s, 3H, CH3 (Mey)), 4.52-4.55 (m, 6.3H), 4.92-4.95 (m, 1.1H), 4.99-5.08 (m, 5H), 5.25-5.30 (m, 3H), 5.41-5.46 (m, 3H), 5.93-6.10 (m, 5.7H), 6.44-6.47 (m, 3.6H), 6.55 (t, J=8.5 Hz, 2H), 7.05 (d, J=8.7 Hz, 1.7H), 7.13 (t, J=8.3 Hz, 1H); GC RI: MS m/z (relative intensity, %): 2-allyl-1-allyloxy-3-methoxy benzene 5by{6,1} 1524: 205 (M++H, 29), 204 (M+, 100); 1-allyl-2-allyloxy-4-methoxy benzene 5bx{6,1} 1554: 205 (M++H, 31), 204 (M+, 100).


5bx,y{6,2-3} Allyl-allyl library B. (Method B, 53% yield): 1H NMR δ: 1.02-1.07 (m, 8.8H, CH3 (Pr)), 1.39-1.42 (m, 9.4H, CH3 (Et)), 1.76-1.85 (m, 6.2H, CH2CH3 (Pr)), 3.34-3.35 (m, 7.7H), 3.46-3.48 (m, 4.3H), 3.88-3.93 (m, 6H, OCH2 (Pr)), 3.98-4.05 (m, 6.5H, OCH2 (Et)), 4.51-4.54 (m, 12.2H), 4.91-4.94 (m, 2H), 5.00-5.07 (m, 10H), 5.24-5.28 (m, 6H), 5.40-5.45 (m, 6H), 5.92-6.09 (m, 12.1H), 6.42-6.45 (m, 7.6H), 6.51-6.54 (m, 4.2H), 7.01-7.03 (m, 3.7H), 7.08-7.11 (m, 2H); GC RI: MS m/z (relative intensity, %): 2-allyl-1-allyloxy-3-ethoxy benzene 5by{6,2} 1581: 219 (M++H, 42), 218 (M+, 100); 1-allyl-2-allyloxy-4-ethoxy benzene 5bx{6,2} 1613: 219 (M++H, 46), 218 (M+, 100); 2-allyl-1-allyloxy-3-propoxy benzene 5by{6,3} 1674: 233 (M++H, 31), 232 (M+, 59), 149 (M−83, 100); 1-allyl-2-allyloxy-4-propoxy benzene 5bx{6,2} 1706: 233 (M++H, 50), 232 (M+, 100).


5bx,y{6,4-5} Allyl-allyl library C. (Method B, 76% yield): 1H NMR δ: 0.96-0.99 (m, 28.1H), 1.45-1.54 (m, 7.5H, CH2CH3 (Bu)), 1.65-1.71 (m, 7H), 1.73-1.92 (m, 10.9H), 3.34-3.36 (m, 6.2H), 3.46-3.47 (m, 3.9H), 3.92-4.00 (m, 12.8H), 4.51-4.55 (m, 10.6H), 4.91-4.94 (m, 2.1H), 5.00-5.07 (m, 9.2H), 5.24-5.29 (m, 5.8H), 5.40-5.45 (m, 5.7H), 5.92-6.10 (m, 11.9H), 6.42-6.45 (m, 6.6H), 6.51-6.55 (m, 4.1H), 7.02-7.04 (m, 3.1H), 7.08-7.12 (m, 2H); GC RI: MS m/z (relative intensity, %): 2-allyl-1-allyloxy-3-butoxy benzene 5by{6,4} 1771: 247 (M++H, 43), 246 (M+, 63), 149 (M−97, 100); 1-allyl-2-allyloxy-4-butoxy benzene 5bx{6,4} 1801: 247 (M++H, 61), 246 (M+, 100); 2-allyl-1-allyloxy-3-(3-methyl-butoxy) benzene 5by{6,5} 1827: 261 (M++H, 74), 260 (M+, 78), 149 (M−111, 100); 1-allyl-2-allyloxy-4-(3-methyl-butoxy)benzene 5bx{6,5} 1861: 261 (M++H, 62), 260 (M+, 100).


Para


5c{2,1-5} Allyl-ethyl library. (Method B, 89% yield): 1H NMR δ: 0.94-0.98 (m, 8.7H), 1.02 (t, J=7.4 Hz, 3.7H), 1.36-1.41 (m, 21H), 1.44-1.52 (m, 2.2H), 1.62-1.66 (m, 4.5H), 1.70-1.86 (m, 5.6H), 3.36-3.38 (m, 10.9H), 3.76 (s, 4H, OCH3), 3.86 (t, J=6.6 Hz, 2.6H), 3.88-3.93 (m, 4H), 3.95-3.99 (m, 14H), 5.03-5.10 (m, 10H), 5.93-6.02 (m, 4.7H), 6.66-6.78 (m, 16.2H, ArH); GC RI: MS m/z (relative intensity, %): 1-allyl-2,5-diethoxy benzene 5c{2,2} 1518: 207 (M++H, 31), 206 (M+, 100); 1-allyl-2-ethoxy-5-propoxy benzene 5c{2,3} 1605: 221 (M++H, 29), 220 (M+, 100); 1-allyl-5-butoxy-2-ethoxy benzene 5c{2,4} 1704: 235 (M++H, 29), 234 (M+, 100); 1-allyl-2-ethoxy-5-(3-methyl-butoxy)benzene 5c{2,5} 1763: 249 (M++H, 27), 248 (M+, 100).


5c{3,1-5} Allyl-propyl library. (Method B, 95% yield): 1H NMR δ: 0.96-1.06 (m, 27.6H), 1.37-1.41 (m, 4H), 1.44-1.53 (m, 2H), 1.64-1.68 (m, 2.9H), 1.72-1.92 (m, 16H), 3.38 (d, J=6.4 Hz, 10.9H), 3.80 (s, 3.8H, OCH3), 3.82-3.99 (m, 19.9H), 4.99-5.18 (m, 10.5H), 5.92-6.05 (m, 5H), 6.67-6.85 (m, 17.5H, ArH); GC RI: MS m/z (relative intensity, %): 1-allyl-2,5-dipropoxy benzene 5c{3,3} 1699: 235 (M++H, 26), 234 (M+, 100); 1-allyl-5-butoxy-2-propoxy benzene 5c{3,4} 1798: 249 (M++H, 27), 248 (M+, 100); 1-allyl-5-(3-methyl-butoxy)-2-propoxy benzene 5c{3,5} 1857: 263 (M++H, 27), 262 (M+, 90), 249 (100).


5c{4,1-5} Allyl-butyl library. (Method B, 95% yield): 1H NMR δ: 0.94-0.98 (m, 19.5H), 1.00-1.04 (m, 3.4H), 1.36-1.39 (m, 3.6H), 1.44-1.54 (m, 9.3H), 1.57-1.58 (m, 2H), 1.64 (t, J=6.8 Hz, 1.8H), 1.70-1.85 (m, 12.5H), 3.35-3.39 (m, 10H), 3.76 (s, 3.7H, OCH3), 3.86 (t, J=6.6 Hz, 2.2H), 3.88-3.93 (m, 11.2H), 3.97 (q, J=6.9 Hz, 2.4H), 5.03-5.17 (m, 9.5H), 5.93-6.06 (m, 4.5H), 6.65-6.87 (m, 16.1H, ArH); GC RI: MS m/z (relative intensity, %): 1-allyl-2,5-dibutoxy benzene 5c{4,4} 1892: 263 (M++H, 28), 262 (M+, 100); 1-allyl-2-butoxy-5-(3-methyl-butoxy)benzene 5c{4,5} 1949: 277 (M++H, 28), 276 (M+, 100).


5c{5,1-5} Allyl-iPentyl library. (Method B, 95% yield): 1H NMR δ: 0.93-0.99 (m, 27.7H), 1.03 (t, J=7.4 Hz, 3.7H), 1.39 (t, J=7.0 Hz, 4H), 1.44-1.52 (m, 2.2H), 1.63-1.88 (m, 18.5H), 3.36-3.39 (m, 10.7H), 3.76 (s, 4H, OCH3), 3.84-3.99 (m, 15.7H), 5.01-5.18 (m, 10.5H), 5.93-6.05 (m, 5H), 6.66-6.85 (m, 17H, ArH); GC RI: MS m/z (relative intensity, %): 1-allyl-2,5-di(3-methyl-butoxy)benzene 5c{5,5} 2001: 291 (M++H, 27), 290 (M+, 100).



1H NMR Data for Individual Compounds (Group II)


1-allyloxy-2-propoxybenzene, 3a{3,6} (5.1 g, 74%): liquid; GC (RI 12.51, 99%); 1H NMR (600 MHz, CDCl3) δ: 6.68-7.15 (m, 4H), 6.14 (ddt, 1H, J=17.3, 10.5, 5.3 Hz), 5.42 (dq, 1H, J=17.3, 1.6 Hz), 5.26 (dq, 1H, J=10.5, 1.6 Hz), 4.60 (dt, 2H, J=5.3, 1.6 Hz), 3.98 (t, 2H, J=6.7 Hz), 1.80-1.91 (m, 2H), 1.05 (t, 3H, J=7.4 Hz). MS m/z (relative intensity): 193 (M++1, 100%), 109 (78%), 81 (42%).


1-allyloxy-2-butoxybenzene, 3a{4,6}, (1.3 g, 98%): 1H NMR (400 MHz, CDCl3) δ: 6.91 (m, 4H), 6.08 (m, 1H), 5.41 (br dd, 1H, J=17.2, 1.2 Hz), 5.26 (br dd, 1H, J=10.4, 1.2 Hz), 4.60 (br d, 2H, J=5.2 Hz), 4.02 (t, 2H, J=6.4 Hz), 1.83 (m, 1H), 1.52 (m, 2H), 0.99 (t, 3H, J=6.8 Hz). MS m/z (relative intensity): 206 (25%), 109 (100%), 81 (26%).


1-propoxy-2-butoxybenzene, 3a{3,4}, (1.0 g, 85%): 1H NMR (400 MHz, CDCl3) δ: 6.90 (br s, 4H), 4.01 (t, 2H, J=6.4 Hz), 3.97 (t, 2H, J=6.8 Hz), 1.84 (m, 4H), 1.51 (m, 2H), 1.05 (t, 3H, J=6.8 Hz), 0.98 (t, 3H, J=7.6 Hz). MS m/z (relative intensity): 208 (27%), 110 (100%).


1-allyloxy-2-isopentoxybenzene, 3a{5,6}, (440 mg, 64%): 1H NMR (400 MHz, CDCl3) δ: 6.90 (m, 4H), 6.08 (m, 1H), 5.42 (m, 1H), 5.26 (m, 1H), 4.59 (dt, 2H, J=5.2, 1.2 Hz), 4.04 (t, 2H, J=6.8 Hz), 1.84 (m, 1H), 1.75 (q, 2H, J=6.8 Hz), 0.97 (d, 6H, J=6.8 Hz). MS m/z (relative intensity): 220 (65%), 150 (25%), 121 (30%), 109 (100%), 43 (32%).


3-propoxy-1-isopentoxybenzene, 3b{3,5} (7.4 g, 57%): liquid; GC (RI 1251, 100%); 1H NMR (400 MHz, CDCl3) δ0.97 (d, 6H, J=6.7 Hz), 1.05 (t, 3H, J=7.4 Hz), 1.69 (q, 2H, J=6.8 Hz), 1.74-1.90 (m, 3H), 3.92 (t, 2H, J=6.6 Hz), 3.97 (t, 2H, J=6.6 Hz), 6.52-6.46 (m, 3H), 7.15 (t, 1H, J=8.1 Hz); MS m/z (relative intensity): 223 (M++1, 100%), 110 (61%).


1-methoxy-2-isopentoxybenzene, 3b{1,5}, (1.3 g, 85%): 1H NMR (400 MHz, CDCl3) δ: 7.17 (t, 1H, J=8.4 Hz), 6.50 (br dt, 2H; J=8.4, 2.4 Hz), 6.46 (br t, 1H, J=2.0 Hz), 3.97 (t, 2H, J=6.8 Hz), 3.79 (s, 3H), 1.82 (m, 1H), 1.67 (dt, 2H, J=6.8, 6.8 Hz), 0.96 (d, 6H, J=6.4 Hz). MS m/z (relative intensity): 194 (20%), 124 (100%), 95 (22%).


1-methoxy-2-allyloxybenzene, 3b{1,6}, (499 mg, 38%): 1H NMR (400 MHz, CDCl3) δ: 7.16 (br t, 1H, J=8.4 Hz), 6.53 (br t, 1H, J=2.0 Hz), 6.50 (m, 2H), 6.05 (m, 1H), 5.41 (br dq, 1H, J=17.2, 1.6 Hz), 5.28 (br dq, 1H, J=10.4, 1.2 Hz), 4.52 (dt, 2H, J=5.2, 1.6 Hz), 3.79 (s, 3H). MS m/z (relative intensity): 164 (5%), 57 (45%), 56 (99%), 41 (100%).


1-allyloxy-2-allyloxybenzene, 3b{6,6}, (1.1 g, 90%): 1H NMR (400 MHz, CDCl3) δ: 7.20 (br dt, 1H, J=5.2, 0.4 Hz), 6.56 (m, 3H), 6.09 (m, 2H), 5.45 (ddd, 2H, J=11.6, 2.4, 1.2 Hz), 5.32 (ddd, 2H, J=6.8, 2.0, 0.8 Hz), 4.55 (dt, 4H, J=3.6, 0.8 Hz). MS m/z (relative intensity): 190 (70%), 120 (30%).


1-allyloxy-3-isopentoxybenzene, 3b{5,6}, (616 mg, 99%): 1H NMR (400 MHz, CDCl3) δ: 7.16 (br t, 1H, J=8.8 Hz), 6.52 (br d, 1H, J=2.4 Hz), 6.49 (m, 2H), 6.06 (m, 1H, J=Hz), 5.42 (dq, 1H, J=17.2, 1.6 Hz), 5.28 (dq, 1H, J=10.4, 1.2 Hz), 4.52 (dt, 2H, J=5.2, 1.6 Hz), 3.97 (t, 2H, J=6.8 Hz), 1.82 (m, 1H), 1.67 (q, 2H, J=6.4 Hz), 0.96 (d, 3H, J=6.4 Hz). MS m/z (relative intensity): 220 (25%), 150 (100%), 149 (30%), 135 (20%), 107 (22%).


1-allyloxy-4-methoxybenzene, 3c{1,6} (10.4 g, 99%): liquid; GC (RI 1251, 97%); 1H NMR (600 MHz, CDCl3) δ: 3.78 (s, 3H), 4.50 (dt, 2H, J=5.3, 1.5 Hz), 5.27 (dq, 1H, J=10.5, 1.4 Hz), 5.40 (dq, 1H, J=17.3, 1.6 Hz), 6.01-6.09 (ddt, 1H, J=17.2, 10.6, 5.3 Hz), 6.81-6.89 (m, 4H); MS m/z (relative intensity): 164 (M+, 38%), 123 (100%), 95 (43%).


1-allyloxy-4-ethoxybenzene, 3c{2,6} (6.2 g, 82%): solid; GC (RI 1251, 98%); 1H NMR (600 MHz, CDCl3) δ: 1.39 (t, 3H, J=7.0 Hz), 3.98 (q, 2H, J=7.0 Hz), 4.48 (dt, 2H, J=5.3, 1.5 Hz), 5.27 (dq, 1H, J=10.5, 1.4 Hz), 5.40 (dq, 1H, J=17.3, 1.6 Hz), 6.05 (ddt, 1H, J=17.2, 10.6, 5.3 Hz), 6.78-6.89 (m, 4H); MS m/z (relative intensity): 179 (M++1, 84%), 178 (M+100%), 137 (74%).


1-allyloxy-4-propoxybenzene, 3c{3,6} (4.2 g, 81%): solid; GC (RI 1251, 100%); 1H NMR (600 MHz, CDCl3) δ: 1.01 (t, 3H, J=7.4 Hz), 1.73-1.80 (m, 2H), 3.85 (t, 2H, J=6.6 Hz), 4.47 (dt, 2H, J=5.3, 1.5 Hz), 5.25 (dq, 1H, J=10.5, 1.4 Hz), 5.38 (dq, 1H, J=17.3, 1.6 Hz), 6.03 (ddt, 1H, J=17.3, 10.5, 5.3 Hz), 6.86-6.79 (m, 4H); 13C NMR (400 MHz, CDCl3) δ: 153.4, 152.6, 133.6, 117.4, 115.7 (2C), 115.3 (2C), 70.1, 69.5, 22.6, 10.5. MS m/z (relative intensity): 193 (M++1, 53%), 192 (M, 88%), 151 (40%), 109 (100%). HRMS-ESI calcd for C12H17O2 (M+H)+, m/z 193.1223, found m/z 193.1215.


1-allyloxy-4-isopentoxybenzene, 3c{5,6}, (510 mg, 84%): 1H NMR (400 MHz, CDCl3) δ: 6.84 (m, 4H), 6.05 (m, 1H), 5.40 (dq, 1H, J=17.2, 1.6 Hz), 5.27 (dq, 1H, J=10.4, 1.2 Hz), 4.49 (dt, 2H, J=5.2, 1.2 Hz), 3.94 (t, 2H, J=6.4 Hz), 1.82 (m, 1H), 1.65 (q, 2H, J=6.8 Hz), 0.96 (d, 3H, J=6.8 Hz). MS m/z (relative intensity): 220 (60%), 150 (24%), 109 (100%), 71 (84%), 43 (82%).


4-ethoxy-1-propoxybenzene, 3c{2,3}, (700 mg, 49%): solid; 1H NMR (400 MHz, CDCl3) δ: 1.02 (t, 3H, J=7.6 Hz), 1.39 (t, 3H, J=7.2 Hz), 1.78 (m, 2H), 3.87 (t, 1H, J=6.4 Hz), 3.98 (q, 1H, J=6.8 Hz), 6.82 (s, 4H). MS m/z (relative intensity): 180 (25%), 138 (20%), 110 (100%), 41 (28%).


1,4-dimethoxy-2-allyl-benzene, 5c{1,1}: (9.4 g, 58% yield of the Claisen rearrangement): liquid, 1H NMR (600 MHz, CDCl3) δ: 3.40 (d, 2H, J=6.7 Hz), 3.80 (s, 3H), 3.82 (s, 3H), 5.14-5.06 (m, 2H), 6.02 (ddt, 1H, J=6.6, 10.1, 16.8 Hz), 6.88-6.70 (m, 3H).


1-methoxy-2-allyl-4-propoxy-benzene, 5c{3,1}: (7.7 g, 81% yield of the Claisen rearrangement): liquid, 1H NMR (600 MHz, CDCl3) δ: 1.03 (t, 3H, J=7.4 Hz), 1.84-1.73 (m, 2H, J=7.5 Hz), 3.37 (d, 2H, J=6.7 Hz), 3.75 (s, 3H), 3.86 (t, 2H, J=6.4 Hz), 5.16-4.98 (m, 2H), 6.06-5.89 (m, 1H), 6.86-6.58 (m, 3H), MS m/z (relative intensity): 208 (100%), 206 (M+, 61%), 164 (41%), 150 (94%), 149 (56%).


1-allyl-2,4-dimethoxybenzene and 1,3-dimethoxy-2-allylbenzene, 5b{1,1} (isomers x and y, ratio 1.8:1), 2.6 g, 79% yield of the Claisen rearrangement, liquid, 1H NMR (600 MHz, CDCl3) δ: 3.37 (d, 2H, J=6.5 Hz, isomer x), 3.48 (d, 2H, J=4.6 Hz, isomer y), 3.92-3.80 (s, 3H, isomers x and y), 5.00 (m, 1H, isomer y), 5.09 (m, 1H, isomer y), 5.16-5.25 (m, 2H, isomer x), 6.10-5.96 (m, isomers x and y), 6.49 (d, 1H, J=8.3 Hz, isomer x), 6.51 (br s, 1H, isomer x) 6.60 (d, 2H, J=8.3 Hz, isomer y), 7.08 (d, 1H, J=8.3 Hz, isomer x), 7.20 (t, 1H, J=8.3 Hz, isomer y), MS m/z (relative intensity): isomer x (retention time 5.81 min): 178 (M+, 100%), 177 (41%), 151 (26%), 149 (28%), 147 (40%), 121 (40%), 91 (27%). isomer y (retention time 5.48 min): 178 (M+, 100%), 149 (57%), 121 (26%), 91 (41%). HRMS-ESI calcd for C11H15O2 (M+H)+, m/z 179.1067, found m/z 179.1061.


1-allyl-4-methoxy-2-propoxy-benzene and 1-methoxy-2-allyl-3-propoxy-benzene, 5b{3,1} (isomers x and y, ratio 1.2:1), 11.1 g, 88% yield of Claisen rearrangement, liquid, 1H NMR (600 MHz, CDCl3) δ: 1.07 (dt, 6H, J=7.4, 1.3 Hz), 1.79-1.87 (m, 4H), 3.35 (d, 2H, J=6.7 Hz), 3.46 (d, 2H, J=6.3 Hz), 3.79-3.81 (m, 3H), 3.82-3.84 (m, 3H), 3.92 (td, 4H, J=9.7, 6.4 Hz), 4.92-5.10 (m, 4H), 5.93-6.04 (m, 2H), 6.42-6.47 (m, 2H), 6.55 (d, 2H, J=8.3 Hz), 7.05 (d, 1H, J=8.1 Hz), 7.14 (t, 1H, J=8.3 Hz). HRMS-ESI calcd for C13H19O2 (M+H)+, m/z 207.1380, found m/z 207.1371.


1-allyl-4-methoxy-2-propoxy-benzene, 5b{3,1} (isomer x), 15 mg, 96% enriched, liquid, 1H NMR of isomer x (600 MHz, CDCl3) δ: 1.05 (t, 3H, J=7.4 Hz), 1.77-1.85 (m, 2H), 3.43 (td, 2H, J=6.3, 1.4 Hz), 3.78 (s, 3H), 3.92 (t, 21-1, J=6.4 Hz), 5.00 (ddd, 1H, J=10.0, 3.6, 1.4 Hz), 5.03 (ddd, 1H, J=17.1, 3.6, 1.4 Hz), 5.95 (tdd, 1H, J=17.1, 10.0, 6.3 Hz), 6.46 (m, 2H), 7.12 (d, 1H, J=8.3 Hz), MS m/z (relative intensity): 206 (M+, 100%), 177 (25%), 164 (38%), 163 (74%), 149 (27%).


1-methoxy-2-allyl-3-propoxy-benzene, 5b{3,1} (isomer y), 48 mg, 100% enriched, 2.6 g 86% enriched, liquid, 1H NMR of pure y (600 MHz, CDCl3) δ: 1.04 (t, 3H, J=7.4 Hz), 1.76-1.84 (m, 2H), 3.30-3.34 (m, 2H), 3.78 (s, 3H), 3.87-3.93 (t, 2H, J=Hz), 4.89-5.06 (m, 2H), 5.90-6.00 (m; 1H), 6.39-6.45 (d, 2H, J=8.3 Hz), 7.02 (d, 1H, J=8.3 Hz). 13C NMR (400 MHz, CDCl3) δ: 158.2, 157.7, 137.0, 127.0, 116.8, 114.0, 104.8, 103.6, 69.9, 55.8, 27.4, 22.8, 10.7. MS m/z (relative intensity): 206 (M+, 100%), 177 (90%), 164 (25%), 163 (30%), 149 (71%), 135 (81%), 133 (34%), 121 (76%), 107 (52%). HRMS-ESI calcd for C13H19O2 (M+H)+, m/z 207.1380, found m/z 207.1370.


1-allyl-4-ethoxy-2-propoxy-benzene and 1-ethoxy-2-allyl-3-propoxy-benzene, 5b{3,2} (isomers x and y, ratio: 2.3:1), 2.6 g, 89% yield of the Claisen rearrangement, liquid, 1H NMR (600 MHz, CDCl3) δ: 1.09 (m, 6H), 1.44 (m, 6H), 1.79-1.92 (m, 4H), 3.36 (m, 2H), 3.50 (m, 2H), 3.94 (m, 4H), 3.99-4.10 (m, 4H), 4.91-5.12 (m, 4H), 6.00 (m, 2H), 6.42-6.56 (m, 4H), 7.00-7.16 (m, 2H). HRMS-ESI calcd for C14H21O2 (M+H)+, m/z 221.1536, found m/z 221.1528.


1-allyl-4-ethoxy-2-propoxy-benzene, 5b{3,2} (isomer x), 13.2 mg, 96% enriched, liquid, 1H NMR (600 MHz, CDCl3) δ: 1.02 (t, 3H, J=7.4 Hz), 1.38 (t, 3H, J=7.0 Hz), 1.72-1.85 (m, 2H), 3.30 (d, 2H, J=6.7 Hz), 3.88 (m, 2H), 4.05 (q, 2H, J=7.0 Hz), 4.94 (ddd, 1H, J=10.2, 2.1, 1.2 Hz), 5.05 (ddd, 1H, J=16.8, 2.4, 1.2 Hz), 5.88-5.99 (m, 1H), 6.38 (dd, 1H, J=8.4, 2.4 Hz), 6.42 (d, 1H, J=2.4 Hz), 6.98 (d, 1H, J=8.4 Hz), MS m/z (relative intensity): 220 (M+, 100%), 191 (25%), 177 (16%), 149 (58%).


1-ethoxy-2-allyl-3-propoxy-benzene, 5b{3,2} (isomer y), 49.7 mg, 100% enriched, liquid, 1H NMR (600 MHz, CDCl3) δ: 1.07 (t, 3H, J=7.4 Hz), 1.42 (t, 3H, J=7.0 Hz), 1.77-1.89 (m, 2H), 3.46 (dt, 2H, J=6.5, 1.3 Hz), 3.94 (t, 2H, J=6.4 Hz), 4.05 (q, 2H, J=7.0 Hz), 4.94 (ddt, 1H, J=10.0, 2.4, 1.3 Hz), 5.05 (ddd, 1H, J=17.0, 3.8, 1.6 Hz), 5.97 (ddt, 1H, J=17.0, 10.0, 6.5 Hz), 6.53 (d, 2H, J=8.3 Hz), 7.11 (t, 1H, J=8.3 Hz). 13C NMR (400 MHz, CDCl3) δ: 157.7, 157.6, 137.1, 126.9, 117.1, 114.0, 104.7, 104.6, 69.8, 63.9, 27.6, 22.8, 15.0, 10.7. MS m/z (relative intensity): 220 (M+, 60%), 191 (62%), 177 (12%), 149 (100%), 135 (46%), 121 (59%), 107 (29%). HRMS-ESI calcd for C14H21O2 (M+H)+, m/z 221.1536, found m/z 221.1532.


1-allyl-4-methoxy-3-isopentoxybenzene and 1-methoxy-2-allyl-3-isopentoxybenzene, 5b{5,1} (isomers x and y, ratio 1.8:1), 11.0 g, 91% yield of the Claisen rearrangement, liquid, 1H NMR (600 MHz, CDCl3) δ: 0.99 (d and s, 12H, J=6.9 Hz), 1.75-1.68 (m, 4H), 1.95-1.85 (m, 2H), 3.34 (d, 2H, J=6.7 Hz), 3.45 (d, 2H, J=6.3 Hz), 3.80-3.82 (m, 3H), 3.83-3.84 (m, 3H), 3.97-4.03 (m, 6H), 4.93-5.09 (m, 4H), 5.93-6.04 (m, 2H), 6.43-6.48 (m, 2H), 6.56 (dd, 2H, J=8.3, 5.3 Hz), 7.05 (d, 1H, J=8.3 Hz), 7.15 (t, 1H, J=8.3 Hz), MS m/z (relative intensity): isomer x (retention time 8.03 min): 234 (M+, 80%), 177 (2%), 164 (95%), 163 (100%), 149 (33%), 147 (37%), 133 (28%). isomer y (retention time 7.83 min): 234 (M+, 100%), 205 (35%), 177 (7%), 164 (72%), 163 (39%), 135 (99%), 133 (41%), 121 (71%), 107 (40%), 77 (28%). HRMS-ESI calcd for C15H23O2 (M+H)+, m/z 235.1693, found m/z 235.1691.


1-allyl-3-allyloxy-4-methoxybenzene and 1-methoxy-2-allyl-3 allyloxybenzene, 5b{6,1} (isomers x and y, ratio 2.3:1), 8.6 g, 69% yield of the Claisen rearrangement, liquid, 1H NMR (600 MHz, CDCl3) δ: 3.37 (br d, 2H, J=6.6 Hz, isomer x), 3.48 (br d, 2H, J=6.0 Hz, isomer y), 3.80 (s, 3H, isomer x), 3.85 (s, 3H, isomer y), 4.54 (ddd, 2H, J=5.4, 1.8, 1.8 Hz, isomer x), 4.55 (ddd, 2H, J=4.8, 1.2, 1.2 Hz, isomer y), 4.90 (m, 1H, isomer y), 5.02 (m, 1H, isomer y), 5.03 (m, 1H, isomer x), 5.07 (m, 1H, isomer x), 5.00-5.10 (m, 2H isomer x+1H isomer y), 5.27 (m, 1H, isomer y), 5.28 (m, 1H, isomer x), 5.44 (ddd, 1H, J=17.4, 3.6, 1.8 Hz, isomer y), 5.45 (ddd, 1H, J=16.8, 3.0, 1.2 Hz, isomer x), 5.95-6.12 (m, 2H, isomer x+2H, isomer y), 6.44-6.49 (m, 2H, isomer x), 6.55 (d, J=8.4 Hz, 1H isomer y), 6.57 (d, J=9.0 Hz, 1H isomer y), 7.04-7.08 (br d, J=9.0 Hz, 2H, isomer x), 7.14 (t, 1H, J=8.4 Hz, isomer y), MS m/z (relative intensity): isomer x (retention time 6.92 min): 204 (M+, 100%), 203 (32%), 177 (9%), 163 (44%), 161 (28%), 135 (43%), isomer y (retention time 6.70 min): 204 (M+, 100%), 189 (26%), 177 (20%), 175 (25%), 163 (85%), 161 (42%), 147 (30%), 135 (89%), 107 (88%), 105 (42%), 103 (50%), 91 (47%), 77 (52%). HRMS-ESI calcd for C13H17O2 (M+H)+, m/z 205.1223, found m/z 205.1232.


For the synthesis of the first set of mini-libraries (Set A, Scheme 1 or 1-1, and Table 2), equimolar mixtures of monoalkoxy 2(a-c){n} compounds were alkylated to afford chemsets of 4 or 5 members 3(a-c){n,1-5}. In order to effect complete deprotonation of the monoalkoxy compounds 2(a-c){n}, the alkylation was conducted with NaH as the base, in DMF, at room temperature. The reaction was monitored by GC and it proceeded at similar rates for all the components, affording crude products of high purity (>90% by GC). However, the removal of DMF resulted in losses of material. Further, the more volatile dialkoxy members 3(a-c){n,1-5} evaporated in sufficient quantities to introduce biases (e.g Table 2, entry 2). Following optimization, the K2CO3/acetone base/solvent system afforded better yields and much less bias (e.g. Table 2, entry 6).









TABLE 2







Purity of the Libraries and % Distribution of the Members in Libraries









Distribution of members



in library (%)d















no.
Librarya
n
Purityb
{n,1}c
{n,2}
{n,3}
{n,4}
{n,5}










Set A















1
3a{1,1-5}
1
100
13.7
13.0
16.9
26.0
30.4


2
3a{2 1-5}
2
99
 7.0
 8.8
15.3
32.5
35.0


3
3a{3,1-5}
3
99
 9.7
12.3
20.2
25.8
30.7


4
3a{4,1-5}
4
99
 9.0
14.7
18.8
27.9
27.2


5
3a{5,1-5}
5
99
 7.7
12.9
17.9
31.2
29.6


6
3a{6,1-5}
6
100
13.2
15.9
19.2
27.3
24.3


7
3b{1,1-5}*
1
99
21.1
21.7
26.1

30.0


8
3b{2,1-5}*
2
95
16.2
20.4
26.4

32.3


9
3b{3,1-5}*
3
97
12.0
16.2
28.6

39.7


10
3b{4,1-5}*
4
100
19.1
20.7
27.7

32.2


11
3b{5,1-5}*
5
97
22.5
22.9
27.3

24.3


12
3b{6,1}
6
100
100  






13
3b{6,2-3}
6
97

62  
38  




14
3b{6,4-5}
6
97



59  
41  


15
3c{1,1-5}*
1
97
15.1
20.2
23.2

38.2


16
3c{2,1-5}*
2
98
20.1
23.6
23.9

30.7


17
3c{3,1-5}*
3
96
19.7
18.6
24.9

32.9


18
3c{4,1-5}*
4
97
24.6
23.0
24.7

24.8


19
3c{5,1-5}*
5
95
22.7
21.2
24.5

26.7


20
3c{6,1-5}
6
100
10.1
13.6
18.6
23.8
33.9







Set B















21
4a{1-5}

95
13.7
17.3
18.9
23.1
22.0


22
4b{1}e

100
61/39






23
4b{2-3}e

100

22/20
32/27




24
4b{4-5}e

100



26/24
28/22


25
4c{1-5}

100
 9.1
14.3
20.6
22.9
33.1







Set C















26
5a{1,1-5}
1
92
12.9
14.5
17.2
24.1
23.7


27
5a{2,1-5}
2
93
14.3
15.5
17.0
23.2
23.1


28
5a{3,1-5}
3
94
14.0
15.2
17.6
23.7
23.1


29
5a{4,1-5}
4
90
16.7
16.7
15.7
22.3
18.5


30
5a{5,1-5}
5
90
19.9
20.7
15.6
19.7
14.5


31
5a{6,1-5}
6
96
17.6
21.5
18.0
22.5
16.4


32
5b{1,1}e
1
99
60/40






33
5b{1,2-3}e
1
96

23/16
38/24


34
5b{1,4-5}e
1
100



34/20
29/17


35
5b{2,1}e
2
100
62/38






36
5b{2,2-3}e
2
98

24/16
38/22




37
5b{2,4-5}e
2
100



36/20
27/17


38
5b{3,1}e
3
100
61/39






39
5b{3,2-3}e
3
100

25/16
35/23




40
5b{3,4-5}e
3
100



35/19
31/15


41
5b{4,1}e,f
4
98
62/38






42
5b{4,2-3}e,f
4
98

34/20
31/15




43
5b{4,4-5}e,f
4
95



30/18
32/20


44
5b{5,1}e,f
5
99
62/38






45
5b{5,2-3}e,f
5
100

34/20
31/15




46
5b{5,4-5}e,f
5
99



30/19
31/20


47
5b{6,1}e,f
6
94
61/39






48
5b{6,2-3}e,f
6
94

36/21
26/17




49
5b{6,4-5}e,f
6
88



29/20
30/21


50
5c{1,1-5}
1
99
11.0
14.0
19.9
23.7
30.0


51
5c{2,1-5}
2
100
12.6
16.5
22.8
21.3
26.8


52
5c{3,1-5}
3
100
12.5
15.9
21.9
22.7
26.9


53
5c{4,1-5}
4
100
 9.8
14.5
23.1
24.1
28.5


54
5c{5,1-5}
5
99
16.5
18.9
22.0
20.3
21.3


55
5c{6,1-5}
6
100
14.1
21.5
27.5
18.6
18.3







Set D















56
6c{1-5}

100
10.5
15.1
19.9
24.6
29.9





*These libraries do not contain the {n,4} member;



aSequence of alkyl substituents in the brackets is interchangeable: e.g. member 3a{1,2} is identical with member 3a{2,1};




bPurity was determined by GC;




c“n” has the same significance as in Scheme 1 and it corresponds to the first number in the bracket of the respective chemset;




dPercent distribution of the library members was determined by GC and validated by NMR and GC-MS data;




eMeta compounds undergoing a Claisen Rearrangement yielded two products, and the “5-alkoxy-2-allyl phenol” (x) is listed first; the same format holds for the alkylated derivatives of the meta Claisen Rearrangement products;




fInitial lot of starting material, 4b{n}, was used completely and re-synthesized as a second lot.







Further expansion of the libraries was accomplished via the ortho-Claisen rearrangement of chemsets 3(a-c){6,1-5} at 180° C. and afforded pure libraries (Set B, Table 2). For the ortho library, 4a{1-5}, traces (2-5%) of the para-Claisen rearrangement products were detected (Scheme 2). For the meta 4b libraries no para-Claisen rearrangement was detected and for para 4c libraries the para-Claisen rearrangement was not possible and not observed (Scheme 2). Under thermal conditions, the para-Claisen rearrangement of allyl phenyl ethers is not an important pathway (Ito, F et al. 2007). Under selected Lewis acid or metal catalysis conditions, and when the ortho positions are blocked the para-Claisen rearrangement can be significant Kuntz et al. 2006; Ollevier et al. 2006; Yadav et al. 2007. The meta compounds 3b{6,1-5} yielded two products: 5-alkoxy-2-allylphenol, x, and 3-alkoxy-2-allylphenol, y (Table 2, Scheme 2) upon ortho-Claisen rearrangement. The rearrangement to the less sterically hindered side was slightly more prevalent (1.4-1.8x) than the alternative rearrangement to the hindered position, consistent with previous literature on the thermal Claisen rearrangement of meta-substituted allyl phenol ethers (Ito, F et al. 2007; Gozzo et al 2003; White and Slater 1961).










The Claisen rearrangement introduced an OH group, which was further alkylated to afford Set C of trisubstituted mini-libraries 5(a-c){n,1-5}. The 4(a-c){1-5} and 5(a-c){n,1-5} mini-libraries can be considered as eugenol (2-methoxy-4-(2-propenyl)phenol) analogues. In one instance, prolonged heating of the 4c{1-5} mini-library afforded the mini-library of racemic dihydrobenzofurans 6c{1-5} which was isolated in 60% yield and 100% purity. Despite many attempts, the ortho and meta sets did not undergo this cyclization reaction upon prolonged heating.


The increment of one carbon between the members of a chemset was reflected in very well resolved peaks in both GC and GC-MS. Members of chemsets belonging to Sets A and C have a common alkyl group, n, and a variable second alkyl group, 1 to 5 (see Scheme 1 or 1-1 for naming). Each chemset contains a member with identical alkyl groups, and these members were synthesized as single compounds and fully identified (1H NMR, 13C NMR and GC-MS). These individual compounds are helpful during screening assays, to obtain information about the molecular mass range and substitution pattern that are best for activity (see below). Data from the 1H NMR spectra of these dialkylated compounds 3(a-c){n,n} and of the monoalkylated phenol compounds 2(a-c){n} was used to assign at least one characteristic signal for each member of a chemset. The proportion of each compound in a set, obtained from the integration of these characteristic signals, was the same as the proportion of that compound obtained by GC. This congruence of 1H NMR and GC data validates the composition of the libraries (Table 2). Each library composition was further confirmed during GC-MS calibrations.


Reaction rates of components in the libraries. Within each set, 2a {1-5}, 2b {1-5} or 2c {1-5}, the rates of the second alkylation were similar for all compounds in the mixture, suggesting that the size of the substituent did not influence the rate of the reaction. This was especially surprising in the case of the ortho substituted substrates for which, regardless of the differences in size of the alkyl substituent or alkyl halide reagent (methyl to iso-pentyl), complete conversion was achieved at the same time for all the members of the set.


To determine whether the Claisen rearrangement of the 3(a-c){6,1-5} libraries was also independent of substituent size, the rearrangement was monitored by GC at regular time intervals. For the para library, 3c{6,1-5}, the GC peaks corresponding to the substrates were better resolved and the percent conversion of four of the starting materials was calculated and plotted against time (FIG. 1). The graph confirms that the size of the substituent did not influence the reaction progress, and that complete conversion of all compounds in a set was achieved after about 9 hours of reaction time. A similar behavior was also obtained for the ortho and meta libraries 3(a,b){6,1-5}. A previous kinetic study of the Claisen rearrangement of various para-substituted allyl phenyl ethers also suggested that the rate of the rearrangement is mildly dependent on the nature of the substituents; electron-releasing groups accelerated the reaction. The methoxy and ethoxy members of that study gave the same rates of rearrangement (Goering and Jacobson 1958).


Comparison between the conversion profile of ortho, meta and para substituted library members showed differences in the half-time to total conversion, but not in the total reaction time. Members of the ortho library 3a{6,1-5} achieved 50% conversion in 1 hour while it took 3 and 4 hours for the members of the para library 3c{6,1-5} and meta library 3b{6,1-5}, respectively, to reach the same point. The time necessary to achieve total conversion was not dependent upon the substitution pattern. For clarity, only data for one member in each library are shown (FIG. 2). In a previous kinetic study, the rearrangement of para and meta methoxy substituted allyl phenyl ether had the same rate constants (Goering and Jacobson 1958).


Dihydrobenzofuran formation. When the para library 3c{6, 1-5} was heated three times longer (30 hours) than required for the completion of the Claisen rearrangement, dihydrobenzofurans 6c{1-5} were obtained. Reported spectral data for the known compound, 6c{1}, was used to confirm the identity of the products (Grant and Liu 2005). As a further proof we synthesized 6c{3} as a single compound, and its spectra as well as GC retention time matched the data for the respective library member. Interestingly, cyclization occurred only on the para substituted compounds 3c{6,1-5} and not on the ortho 3a{6,1-5} or meta 3b{6,1-5} substituted ones. Ortho and meta allyl ethers began decomposing when heated longer than was necessary to complete the Claisen rearrangement. Further, we learned that the cyclization reaction followed the Claisen rearrangement and, therefore library 6c{1-5} could also be obtained directly from the 4c{1-5} library. The cyclization reaction proceeded in a Markovnikov sense, and this selectivity has been observed also with (3′-methyl)-2′-butenyl (dimethylallyl) substituents (Ollevier et al. 2006). In previous literature, allyl aryl ethers were rearranged and cyclized to dihydrobenzofurans in the presence of a copper (II) triflate catalyst (Ito et al. 2007), an iridium (III)/silver triflate catalyst (Grant and Liu 2005), aluminum-containing mesoporous molecular sieves (Mathew et al. 2004), a gold (I)-catalyst (Reich et al. 2006) or a bismuth triflate catalyst (Ollevier et al. 2006). These studies also suggest that the Claisen rearrangement occurs first, followed by the Markovnikov addition of the new phenol OH to the allyl double bond (Reich et al. 2006). In fact, few catalysts promoted the tandem reaction; some only catalyzed the Claisen reaction and others caused decomposition. Further, the allyl phenyl ethers that cyclized best, generally had electron-releasing groups or no additional substituents on the benzene ring.


Preparation of Compounds Group II


For the meta compounds, the Claisen rearrangement gave two isomers. For the alkoxy substituents that were used, the isomer in which the allyl group migrates to position 4 (isomer x) is slightly favoured thermodynamically over the isomer in which the allyl group migrates to position 2 (isomer y) (White and Slater 1961; Gozzo et al. 2003). Typical ratios of compounds x:y range from 2.3:1 to 1.2:1. Isomers x and y from the Claisen rearrangement of meta substituted allyloxybenzenes were separated by flash chromatography on AgNO3-silica.


The two isomers x and y were separated for selected cases of series 5b (see Table 7). Briefly, 1% (w/v) AgNO3 was dissolved in water, to which was added silica gel to form a thick slurry. The slurry dried overnight (120° C.), before being packed into the column. Care was taken not to expose the silver nitrate silica to light, by wrapping the beaker with the slurry and later the column with aluminum foil. The silver-silica column was equilibrated with hexane-toluene: 99:1, and the loaded compounds were eluted with 90:10 hexane-toluene. To monitor the separation, 1% AgNO3 TLC plates were prepared by running the silver nitrate solution up the plates and drying them. The plates could be stained with anisaldehyde solution. Isomer y ran faster than x, and it was possible to obtain several fractions that contained pure y. However, y also tailed into the x peak, so that it was not possible to obtain fractions with 100% x by FCC. Alternatively, 5b{3,1}y and 5b{3,1}x as well as 5b{3,2}y and 5b{3,2}x could be separated by preparative TLC (100% hexanes) with multiple developments.


The more compact isomer y was more volatile than x, eluting usually 0.5-1 min earlier from the GC (DB-5 column). Also, in general, isomer y formed an M+1 ion in the mass spectrum more readily and fragmented more extensively (for example, to the tropylium ion m/z 91) than isomer x.


Example 2
Testing of Compounds for Toxicity, Oviposition and Feeding

Materials and Methods


Plant Material


Cabbage plants (Brassica oleraceae var. Stonehead) used in the bioassays were routinely grown in plastic pots with a mixture of sandy loam soil and peat moss (4:1) in a greenhouse at the University of British Columbia, Vancouver, BC, Canada. Leaves were collected from cabbage plants that were 5-6 weeks old.


Test Insects



T. ni larvae and moths were obtained from a long established colony (>50 generations) maintained on an artificial diet, Velvetbean Caterpillar Diet No. F9796 [Bio-Serv Inc. (Frenchtown, N.J.)] in the insectary of the University of British Columbia (UBC). The diet was supplemented with finely ground alfalfa, to improve acceptability, and vitamins [No. 8045; Bioserv Inc. (Frenchtown, N.J.)].


General Testing Procedure


Initial screening for feeding deterrent effects was conducted at 50 μg/cm2 in feeding deterrent bioassays. Compounds that exhibited >50% feeding deterrence at this concentration were subjected to further testing for oviposition deterrent effects and contact toxicity at 0.25% of the test substance. For compounds exhibiting ≧50% values for feeding deterrence and ≧70% mortality by contact, DC50 (concentration causing 50% feeding deterrence compared with the control) and LC50 (concentration causing 50% mortality compared with the control) were determined, respectively, based on bioassays involving a minimum of four concentrations (3.12-25 μg/cm2).


Feeding Deterrence Bioassays


Leaf disk choice bioassays (Akhtar et al. 2003; Akhtar and Isman 2004) were conducted to determine feeding deterrent effects of the synthetic compounds using freshly molted third instar larvae starved for 4-5 h prior to each bioassay. Larvae were given the choice of feeding on two leaf disks, one treated with 10 μL of a solution of the test substance painted on each side and the other treated with a carrier solvent alone. The number of larvae was 25 per treatment. Bioassays were terminated when approximately 50% of the control disk had been eaten (normally 3-5 h). Areas of control and treated leaf disks consumed by the larvae were measured using Scion Image software, and feeding deterrence was calculated (Akhtar and Isman 2004) using the formula [(C−T)/(C+T)]×100, where C and T are areas consumed of the control and treated leaf disks, respectively.


Oviposition Deterrence Bioassays


Oviposition response of T. ni moths was measured according to the oviposition choice bioassay described in Akhtar and Isman 2003 and Chow et al. 2005. T. ni larvae were reared on normal diet from neonates (<24 h old) until pupation. Pupae were sexed and put in separate plastic containers until emergence. After eclosion, pairs of moths (one male and one female) were introduced into each cage with a control and a treated cabbage leaf. Pairs of moths (n=25) were used per treatment. Each leaf (approximately 100-110 cm2) was sprayed with 0.5 mL of MeOH or a methanolic solution of the test chemical on each side. Eggs were counted on each cabbage leaf after 48 h. ODI (oviposition deterrence index) was calculated using the formula ODI=[(C−T)/(C+T)]×100, where C and T are the numbers of eggs laid on the control and treated leaf disks, respectively (Akhtar and Isman 2003; Chow et al. 2005).


Contact Toxicity Bioassay


Mortality was determined 24 h after spraying larvae directly with test solutions. Third instar T. ni larvae were sprayed in 90 mm×15 mm Petri dishes (Falcon) lined with Fisher Scientific filter paper (90 mm diameter). Small plastic hand spraying bottles (50 mL capacity) were used. Larvae were then transferred to Petri dishes (90 mm×15 mm) with a small piece of artificial diet. Each Petri dish contained 10 larvae. Three replicates, each consisting of 10 larvae, were used per treatment.


Comparison of Toxicity, Oviposition, and Feeding Deterrence Values


The mortality of each test material was plotted against its respective oviposition deterrence value (determined at 0.25%) to explore the relationship between the two bioassays using correlation analysis. Similarly, feeding deterrence was plotted against oviposition deterrence and mortality.


Data Analysis


Feeding deterrence data (percent) for initial screening concentration were analyzed by analysis of variance (ANOVA) after arcsin transformation using statistics software (Statistix 7. Analytical Software, Statistix 7 for Windows 95, 98, NT, 2000. Analytical Software, Tallahassee). Where significant F values were found, Tukey's HSD multiple comparison tests were used to test for significant differences between individual treatments.


Results for Test Compound Group 1
Example 3
Effects of p-Dialkoxybenzene Libraries, Pure Compounds, and 1-Hydroxy-4-alkoxy Compounds

All six of the p-dialkoxybenzene libraries and five individual compounds (3c{R1;R2} exhibited >50% feeding deterrence at the initial screening concentration (50 μg/cm2) and, therefore, were subjected to further testing (Table 3) against third instar T. ni larvae for toxic and oviposition deterrent effects. The response of the larvae to the initial screening concentration varied significantly in most cases (one-way ANOVA; F16405=9.6, p<0.0001).


Feeding Deterrence Effects

1-Isopentyloxy-4-alkoxybenzene 3c{5,1-5} had the lowest DC50 value (8.5 μg/cm2) followed by 1-butyloxy-4-alkoxybenzene 3c{4,1-5} (14.5 μg/cm2) and 1-allyloxy-4-isopentoxybenzene 3c{5,6} (15.7 μg/cm2) (Table 3). 1-Hydroxy-4-methoxybenzene 2c{1} and 1-hydroxy-4-propoxybenzene 2c{3} acted as feeding stimulants at the screening concentration. 1-Hydroxy-4-ethoxybenzene 2c{2} (a precursor to diethyl and the ethyl minilibrary) was a weak feeding deterrent.


Toxic Effects

1,4-Diethoxybenzene 3c{2,2} and the 1-ethoxy-4-alkoxybenzene 3c{2,1-5} library were the most toxic (Table 3) at 0.25% (LC50 values were 0.03% for both) followed by 1-butyloxy-4-alkoxybenzene 3c{4,1-5} and 1-propoxy-4-alkoxybenzene 3c{3,1-5}.









TABLE 3







Bioactivities of 1,4-dialkoxybenzene libraries and analogues


(3c{R1; R2}) against third instar T. ni larvaea


























FD (%),
DC50, μg/cm 2

OD (%),





mean ± SE
(r2)b
mortality (%)
mean ± SE


compound
R1
R2
(n = 25)
(n = 25)
(n = 3 × 10)
(n = 25-33)





1,4-dimethoxybenzene
CH3
CH3
 9.9 ± 18.0cd
-c




1,4-diethoxybenzene
CH3
C2H5
 80.8 ± 11.3b
25.9 (0.91) 20.3
100.0d
74.7 ± 13.3a 


1,4-dipropoxybenzene
C3H7
C3H7
 96.0 ± 2.8a
(0.99)
22.6
11.8 ± 13.1b 


1,4-diisopentoxybenzene
C5H11
C5H11
 44.0 ± 18.3abc





1,4-diallyloxybenzene
C3H5
C3H5
 96.9 ± 3.6a
23.9 (0.97)
16.0
14.1 ± 13.1b 


Me library (1-methoxy-4-alkoxybenzene)
CH3
CH3, C2H5, C3H7, C5H11
 80.2 ± 9.8b
34.6 (0.90)
23.3
6.0 ± 5.1b 


Et library (1-ethoxy-4-alkoxybenzene)
C2H5
CH3, C2H5, C3H7, C5H11
 90.7 ± 10.2a
23.4 (0.99)
96.8e
56.6 ± 16.9ab


Pr library (1-propoxy-4-alkoxybenzene)
C3H7
CH3, C2H5, C3H7, C5H11
 53.4 ± 15.8abc
39.7 (0.94) 14.5
53.1
9.6 ± 13.8b


Bu library (1-bulytoxy-4-alkoxybenzene)
C4H9
CH3, C2H5, C3H7, C5H11
 83.4 ± 9.7b
(0.83)
58.1
50.1 ± 14.5ab


iPent library (1-isopentyloxy-4-
C5H11
CH3, C2H5, C3H7, C5H11
100.0 ± 0.0a
5.8 (0.85)
18.8
22.9 ± 14.2ab


alkoxybenzene allyl small








library (1-allyloxy-4-alkoxybenzene)
C3H7
CH3, C2H5C3H7
 82.4 ± 10.7b
27.9 (0.93)
10.0
5.4 ± 13.9b


1-allytoxy-4-butoxybenzene
C3H7
C4H9
 84.0 ± 10.7b
22.6 (0.90) 15.7
10.0
16.8 ± 13.8ab


1-allyloxy-4-isopentoxybenzene
C3H7
C5H11
 75.1 ± 12.7abc
(0.90)
7.0
18.8 ± 14.3ab


1-hydroxy-4-methoxybenzene
H
CH3
−26.5 ± 18.6d





1-hydroxy-4-ethoxybenzene
H
C2H5
 29.8 ± 18.2bcd





1-hydroxy-4-propoxybenzene
H
C3H7
−28.0 ± 18.4d





1-hydroxy-4-isopentoxybenzene
H
C5H11
 29.5 ± 16.6bcd








a Feeding deterrent (FD) effects (mean ± SE) at 50 μg/cm2are expressed in %.


DC50s (concentrations causing 50% feeding deterrence compared with the control) were calculated for samples showing >50% feeding deterrence in initial screening (≧50 μg/cm2) using Excel: linear regression analysis was conducted for all dose-response experimental data.


Mortality and oviposition deterrent effects were determined at 0.25% for samples showing >50% feeding deterrence in initial screening.


LC50(concentrations causing 50% mortality compared with the control) was calculated for test compounds exhibiting ≧70% mortality at 0.25%.


Means followed by the same letters within a column do not differ significantly (Tukey's test, p < 0.05).


b Coefficient of determination.


c Not tested.


d LC50 = 0.03%.


e LC50 = 0.03%.


f Precursor to diethyl and the ethyl minilibrary.






Oviposition Deterrence Effects

1,4-Diethoxybenzene 3c{2,2}, the 1-ethoxy-4-alkoxybenzene library 3c{2,1-5}, and the 1-butyloxy-4-alkoxybenzene 3c{4,1-5} library showed the strongest oviposition deterrent effects (74.7%, 56.6%, and 50.1%, respectively) when tested at 0.25% (Table 3). Other members in the group demonstrated weak oviposition deterrent effects (<23%). Responses of moths varied significantly in most cases (one-way ANOVA; F10304=2.8, p<0.003).


Example 4
m-Dialkoxybenzene Libraries, Pure Compounds, and 1-Hydroxy-3-alkoxy Compounds

All five of the m-dialkoxybenzene libraries and two pure compounds (3b{R1;R2}) exhibited >50% feeding deterrence in initial screening (50 μg/cm2) and therefore were subjected to further testing (Table 4). The response of the larvae to initial screening concentration varied significantly in most cases (one-way ANOVA; F12307=8.6, p<0.0001).


Feeding Deterrence Effects

The 1-butoxy-3-alkoxybenzene 3b{4,1-5} library had the lowest DC50 value (14.4 μg/cm2) followed by the 1-isopentoxy-3-alkoxybenzene 3b{5,1-5} library (DC50) 19.8 μg/cm2). Three compounds acted as feeding stimulants to third instar T. ni larvae.


Toxic Effects

1,3-Dipropoxybenzene 3b{3,3} was the most toxic (Table 4) at 0.25% and had a LC50 value of 0.16% followed by the 1-propoxy-3-alkoxybenzene library 3b{3,1-5} (50% mortality).


Oviposition Deterrence Effects

The 1-methoxy-3-alkoxybenzene 3b{1,1-5} library demonstrated the strongest oviposition deterrent effect (70.2%) followed by the 1-isopentoxy-3-alkoxybenzene 3b{5,1-5}library (35.7%) (Table 4) when tested at 0.25%. Responses of moths varied significantly in most cases (one-way ANOVA; F6199=2.19, p<0.04).









TABLE 4







Bioactivities of 1,3-Dialkoxybenzene Libraries and Analogues


(3b{R1; R2}) against Third Instar T. ni Larvaea


























FD (%),
DC50, μg/

OD (%),





mean ± SE
cm 2 (r2)b
mortality (%)
mean ± SE


compound
R1
R2
(n = 25)
(n = 25)
(n = 3 × 10)
(n = 25-33)





1,3-dimelhoxybenzene
CH3
CH3
 20.4 ± 19.1bcde
−c




1.3-diethoxybenzene
C2H5
C2H5
 89.4 ± 8.3ab
28.7 (0.85)
36.7
3.4 (14.1b


1,3-dipropoxybenzene
C3H7
C3H7
 96.9 ± 3.1a
26.8 (0.79)
76.7d
33.0 (14.6ab


1,3-diisopentoxybenzene
C5H11
C5H11
−12.2 ± 18.8de





Me library (1-methoxy-3-alkoxybenzene)
CH3
CH3, C2H5, C3H7, C5H11
 54.8 ± 16.6abcd
61.5 (0.94)
16.7
70.2 (17.3a


Et library (1-ethoxy-3-alkoxybenzene)
C2H5
CH3, C2H5, C3H7, C5H11
 69.8 ± 13.8abc
26.5 (0.98)
20.0
14.3 (13.5ab


Pr library (1-propoxy-3-alkoxybenzene)
C3H7
CH3, C2H5, C3H7, C5H11
 98.0 ± 1.5a
21.5 (0.96)
50.0
2.7 (14.2b


Bu library (1-butyloxy-3-alkoxybenzene)
C4H9
CH3, C2H5, C3H7, C5H11
 84.1 ± 8.9ab
14.4 (0.85)
30.0
25.9 (13.9ab


iPent library (1-isopentyloxy-3-alkoxybenzene
C5H11
CH3, C2H5, C3H7, C5H11
 82.6 ± 12.0ab
19.8 (0.96)
20.8
35.7 (13.4ab


1-hydroxy-3-methoxybenzene
H
CH3
 44.7 ± 17.5abcde





1-hydroxy-3-ethoxybenzene
H
C2H5
−18.7 ± 17.8e





1-hydroxy-3-propoxybenzene
H
C3H7
 4.0 ± 20.4cde





1-hydroxy-3-isopenloxybenzene
H
C5H11
 −5.8 ± 18.7de










a
Feeding deterrent (FD) effects (mean ± SE) at 50 μg/cm2are expressed in %.



DC50s (concentrations causing 50% feeding deterrence compared with the control) were calculated for samples showing >50% feeding deterrence in initial screening concentration (≧50 μg/cm2) using Excel; linear regression analysis was conducted for all dose-response experimental data.


Mortality and oviposition deterrent effects were determined at 0.25% for samples showing >50% feeding deterrence in initial screening.


LC50(concentrations causing 50% mortality compared with the control) was calculated for test compounds exhibiting ≧70% mortality at 0.25%.


Means followed by the same letters within a column do not differ significantly (Tukey's test, p < 0.05).




b
Coefficient of determination.





c
Not tested.



d LC50= 0.16%.






Example 5
o-Dialkoxybenzene Libraries, Pure Compounds, and 1-Hydroxy-2-alkoxy Compounds

Six o-dialkoxybenzene libraries and three individual compounds (3a{R1;R2}) exhibited >50% feeding deterrence in initial testing and therefore were subjected to further testing (Table 5) as explained above. The response of the larvae to initial screening concentration varied significantly in most cases (one-way ANOVA; F16401=5.4, p<0.0001).


Feeding Deterrence Effects


The 1-butoxy-2-alkoxybenzene 3a{4,1-5} library had the lowest DC50 value (16.8 μg/cm2) followed by the 1-propoxy-2-alkoxybenzene library (DC50=19.4 μg/cm2).


Toxic Effects


None of the o-dialkoxybenzene libraries or pure compounds caused >40% mortality at 0.25% (Table 5).


Oviposition Deterrence Effects


The 1-allyloxy-2-alkoxybenzene 3a{6,1-5} library demonstrated strong oviposition deterrent activity (66.7%) at 0.25%. The 1-allyloxy-2-alkoxybenzene library demonstrated strong oviposition deterrent activity (66.7%) at 0.25%. Other libraries and compounds had modest oviposition deterrent activities (Table 5; one-way ANOVA; F7205=1.07, p=0.38).









TABLE 5







Bioactivities of 1,2-Dialkoxybenzene Libraries and Analogues


(3a{R1; R2}) against Third Instar T. ni Larvaea


























FD (%),
DC50, μg/

OD (%),





mean ± SE
cm 2 (r2)b
mortality (%)
mean ± SE


compound
R1
R2
(n = 25)
(n = 25)
(n = 3 × 10)
(n = 25-33)





1,2-dimethoxybenzene
CH3
CH3
 26.0 ± 17.6abcd
−c




1,2-diethoxybenzene
C2H5
C2H5
 −2.1 ± 18.6bcd





1,2-dipropoxybenzene
C3H7
C3H7
 26.2 ± 17.6abcd





1,2-dibutoxybenzene
C4H9
C4H9
 56.4 ± 13.8abcd
43.8 (0.90)
10.0
19.6 (14.4a


1,2-diisopentoxybenzene
C5H11
C5H11
 69.9 ± 11.5abc
19.6 (0.96)
40.0
11.5 (13.8a


1,2-diallyloxybenzene
C3H5
C3H5
 70.4 ± 13.2abc
22.4 (0.99)
20.0
15.0 (16.6a


Me library (1-methoxy-2-
CH3
CH3, C2H5, C3H7, C4H9, C5H11
 23.5 ± 15.9abcd





alkoxybenzene)








Et library (1-ethoxy-2-alkoxybenzene)
C2H5
CH3, C2H5, C3H7, C4H9, C5H11
 71.0 ± 10.6ab
24.1 (0.95)
 6.7
28.7 (16.4a


Pr library (1-propoxy-2-alkoxybenzene)
C3H7
CH3, C2H5, C3H7, C4H9, C5H11
100.0 ± 0.0a
19.4 (0.89)
13.8
31.7 (17.4a


Bu library (1-butoxy-2-alkoxybenzene)
C4H9
CH3, C2H5, C3H7, C4H9, C5H11
 98.0 ± 1.9a
16.8 (0.90)
 6.7
28.7 (16.7a


iPent library (1-isopentoxy-2-
C5H11
CH3, C2H5, C3H7, C4H9, C5H11
 66.9 ± 12.7abc
32.5 (0.90)
10.0
29.4 )16.7a


alkoxybenzene)








allyl library (1-allyloxy-2-
C3H7
CH3, C2H5, C3H7, C4H9, C5H11
 67.8 ± 13.5abc
30.0 (0.90)
23.3
66.7 (16.9a


alkoxybenzene)








1-hydroxy-2-allyloxybenzene
H

 31.1 ± 15.5abcd





1-hydroxy-2-methoxybenzene
H
CH3
 −7.5 ± 19.8cd





1-hydroxy-2.butoxybenzene
H
C4H9
 12.0 ± 20.3bcd





1-hydroxy-2-ethoxybenzene
H
C2H5
 −5.3 ± 19.8bcd





1-hydroxy-2-propoxybenzene
H
C3H7
−12.0 ± 20.0e









aFeeding deterrent (FD) effects (mean ± SE) at 50 μg/cm2are expressed in %.



DC50s (concentrations causing 50% feeding deterrence compared with the control) were calculated for samples showing >50% feeding deterrency in initial screening (≧50 μg/cm 2) using Excel; linear regression analysis was conducted for all dose-response experimental data.


Mortality and oviposition deterrent effects were determined at 0.25% for samples showing >50% feeding deterrency in initial screening.


Means followed by the same letters within a column do not differ significantly (Tukey's test, p < 0.05).



bCoefficient of determination.




cNot tested.







Example 6
Example 7
Comparison of Toxicity, Oviposition, and Feeding Deterrence Values of Test Compounds (Group I)

Toxicity and oviposition deterrence: There was a very slight positive correlation (y=0.33x+18.0, R2=0.18) although there were some strong deterrents that were not toxic in the data set. Feeding deterrence and oviposition deterrence: There was no correlation (y=−0.26x+48.0, R2=0.04) within the data set. Feeding deterrence and toxicity: There was no correlation (y=0.07x+77.0, R2=0.01) within the data set.









TABLE 6





Summary of Exemplary Feeding and Oviposition Deterrents, Grouped According to Their Contact Toxicity to Third Instar T. ni Larvaea




















Toxic lead compounds and mini libraries






























Feeding
strong
strong
strong
strong
strong


deterrency







Oviposition
strong
moderate
strong
strong
none


deterrency















R2 = Me, Et, Pr, isopentyl *moderate toxicity (58% mortality)















Low toxicity lead compounds and mini libraries






























Feeding
strong
strong
strong
strong
strong


deterrency







Oviposition
weak
weak
weak
none
weak


deterrency













































Feeding
strong
strong
strong
moderate


deterrency






Oviposition
moderate
moderate
moderate
strong





R2= Me, Et, Pr, Bu, isopentyl



aCompounds with >80% mortality were considered toxic and compounds with <25% mortality were considered of low toxicity. See Tables 1-3 for the activity data. Strong feeding deterrency, >80%; >60%. Strong oviposition deterrency, >50%; moderate, >25%; weak, >10%; none, <10%.







Example 10
Testing of Compounds of Group II for Toxicity, Oviposition and Feeding Deterrence

Individual Compounds or Compound Sets (Group II)


All four of the dialkoxybenzene sets and thirteen individual compounds along with DEET exhibited >50% feeding deterrence at the initial screening concentration (50 μg/cm2) and, therefore, were subjected to further testing (Table 8) against third instar T. ni larvae for toxic and oviposition deterrent effects. The response of larvae to sets or compounds at the initial screening concentration varied significantly in most cases (one-way ANOVA; F18.462=4.4, p<0.0001).


DC50 values varied from 0.5-42.1 μg/cm2 (Table 8). The compound 1-allyloxy-4-propoxybenzene, 3c{3,6}, had the lowest DC50 value (0.5 μg/cm2) followed by the 1-allyloxy-4-alkoxybenzene set (8.5 μg/cm2), 3c{6,1-5}, which contains compound 3c{3,6}. DEET showed a DC50 value of 46.7 μg/cm (Table 8).


At 0.25% the 1-allyloxy-3-ethoxy/isopropoxybenzene set 3b{6,2-3} was the most toxic (65% mortality) followed by the 1-allyloxy-3-butoxy/isopentoxybenzene set 3b{6,4-5} (40% mortality) (Table 8). 1-Allyloxy-4-ethoxybenzene 3c{2,6} was the least toxic (6.7% mortality) in this group.


At 0.25% the 1-allyloxy-2-alkoxy benzene set 3a{6,1-5} showed the strongest oviposition deterrent effect (66.7%) (Table 8). Members of set 3c{6,1-5} with small alkoxy substituents (3c{1,6}, 3c{2,6} and 3c{3,6}) were poor oviposition deterrents (<30%). The meta substituted dialkoxybenzenes (3b compounds) were generally weak, with the strongest congeners being the ones with a molecular volume of 250-260 Å3 and either an allyloxy or an isopentyloxy group (3b{1,5} and 3b{6,6} (25-30% oviposition deterrence).









TABLE 7







Libraries of ortho Claisen rearrangement products from 1-allyloxy-3-alkoxybenzenes.



























DC50c







F.D (%)b
μg/cm2
Mortality
OD (%)d


Compound/


Mean ± SE
(R2)
(%)d
Mean ± SE


setsa
R1a
R2a
N = 25
N = 25
N = 3 × 10
N = 35-40
















4b{1}
Me
H
 74 ± 11ABC
25 (0.98)
20
12 ± 12


4b{2-3}
Et, Pr
H
 73 ± 11ABC
33 (0.99)
70
31 ± 14


4b{4-5}
Bu, iPent
H
 66 ± 13ABC
26 (0.96)
50
45 ± 15


5b{1,1}
Me
Me
 77 ± 9ABC
25 (0.99)
50
−5 ± 13


5b{1,2-3}
Et, Pr
Me
 74 ± 11ABC
23 (0.98)
65
19 ± 15













5b{1,4-5}
Bu, iPent
Me
 41 ± 16ABC
















5b{2,1}
Me
Et
 93 ± 5AB
24 (0.89)
3.6
−2 ± 13


5b{2,2-3}
Et, Pr
Et
 79 ± 10ABC
17 (0.99)
20
28 ± 14


5b{2,4-5}
Bu, iPent
Et
 81 ± 11ABC
15 (0.95)
10
−24 ± 13 


5b{3,1}
Me
Pr
100 ± 0A
16 (0.90)
3.6
−11 ± 15 


5b{3,2-3}
Et, Pr
Pr
 93 ± 5AB
8.7 (0.85)
10
 5 ± 13













5b{3,4-5}
Bu, iPent
Pr
 33 ± 16BC


34 ± 15













5b{3,2}
Et
Pr
 92 ± 6AB
17 (0.79)
0.6
25 ± 13


5b{4,1}
Me
Bu
 60 ± 16ABC
27 (0.90)
10
13 ± 14


5b{4,2-3}
Et, Pr
Bu
 54 ± 16ABC
49 (0.96)
6.7
 9 ± 13













5b{4,4-5}
Bu,iPent
Bu
 26 ± 16C


18 ± 14













5b{5,1}
Me
iPent
 71 ± 14ABC
19 (0.93)
16
51 ± 14


5b{5,2-3}
Et, Pr
iPent
 79 ± 9ABC
24 (0.98)
6.7
15 ± 14













5b{5,4-5}
Bu, iPent
iPent
 41 ± 17ABC
















5b}6,1}
Me
allyl
 76 ± 12ABC
21 (0.97)
25
50 ± 14


5b{6,2-3}
Et, Pr
allyl
 90 ± 8AB
16 (0.96)
33
 8 ± 13


5b{6,4-5}
Bu, iPent
allyl
 57 ± 15ABC
43 (0.96)
33
19 ± 13










Second lots and purified isomers of 5b{n,1} or 5b{3,2} compounds
















5b{1,1}e
Me
Me
 58 ± 15ABC
39 (0.97)
−2.9
10 ± 15


5b{3,1}e
Me
Pr
 91 ± 6AB
26 (0.96)
10
 .7 ± 11


5b{3,1}yf
Me
Pr
100 ± 0A
14 (0.98)
−4.2
17 ± 14


(100% y)








5b{3,1}xf
Me
Pr
 68 ± 12ABC
24 (0.76)
−32
0


(68%) x, 32% y)








5b{3,2}g
Et
Pr
 92 ± 6AB
17 (0.79)
0.6
25 ± 13


5b{3,2}yf
Et
Pr
 74 ± 14ABC
27.6 (0.99) 
0
−6.4 ± 11.4


(100% y)








5b{3,2}yf
Et
Pr
 95 ± 3AB
25 (0.98)
0
26 ± 8 


(82% x, 18% y)








5b{5,1}e
Me
iPent
 99 ± 1A
 4 (0.98)
6.0±
32 ± 12


5b{6,1}e
Me
allyl
 92 ± 6AB
23 (0.92)
−1.6±
10 ± 14






aMe = methyl, Et = ethyl, Pr = propyl, Bu = n-butyl, iPent = isopentyl (= 3-methylbutyl). For 5b sets, the code is 5b{R2, R1} (Scheme 1). The compounds are a mixture of isomers x and y in a ratio of x: y 2: 1.




bFeeding deterrent effects (Mean ± SE) at 50 μg/cm2are expressed in %. Means followed by the same capitalized letters within a column do not differ significantly (One-way ANOVA, F27.684= 3.2, p < 0.0001; Tukey's test, p < 0.05).




cDC50s (concentrations causing 50% feeding deterrence compared with the control) were calculated for samples showing >50% feeding deterrence in initial screening concentration (50 μg/cm2), using Excel. Linear regression analysis was conducted for all dose-response experimental data. The R2values for the linear regressions are shown in parenthesis after the number.




dMortality and oviposition deterrent (OD) effects were determined at 0.25% for samples showing >50% feeding deterrence in initial screening.




e These lots were prepared on a larger scale than previously (22), and the ratios of x: y were: 5b{1,1} 1.1.8: 1, 5b{3,1} 1.2: 1, 5b{3,2} 2.3: 1, 5b{5,1} 1.8: 1, 5b{6,1} 2.3: 1.




fThe isomers were separated on a column of silica/silver nitrate (see methods). gSame set as listed above with the sets, provided for convenience.



—not tested













TABLE 8







Activity of individual compounds or compound sets of compound set


B or precursor sets for the Claisen rearrangements and subsequent alkylations.


The activities of the products of those reactions are shown in Tables 9,


10 and 7 Compounds were synthesized, as shown in Scheme 1A.









a = ortho, OR2 at position 2


b = meta, OR2 at position 3


c = para, OR2 at position 4




















DC50c


















F.D (%)b
μg/cm2
Mortality
OD (%)d





Mean ± SE
(R2)
(%)d
Mean ± SE


Compound/setsa
R1a
R2a
N = 25
N = 25
N = 3 × 10
N = 35-40
















3a{3,6}
Allyl
Propyl
 96 ± 3A
12 (0.98)
17
28 ± 13


3a{3,6}


 97 ± 15A
16 (0.92)
10
12 ± 13


3a{4,6}
Allyl
Butyl
 92 ± 6A
17 (0.96)
19
35 ± 17


3a{3,4}
Propyl
Butyl
 98 ± 2A
21 (0.97)
19
37 ± 14


3a{6,1-5}
Allyl
Me, Et, Pr, Bu,
 68 ± 12AB
30 (0.90)
23
67 ± 15




iPent

















3a{5,6}
Allyl
iPent



 26 ± 13.5













3b{3,5}
Propyl
iPent
 97 ± 3A
17 (0.98)
10
 9 ± 17


3b{1,5}
Methyl
iPent
 89 ± 7A
29 (0.96)
30
30 ± 14


3b{1,6}
Methyl
Allyl
 66 ± 12AB
34 (0.89)
33
9 ± 14


3b{6,2-3}
Allyl
Et, Pr
 64 ± 12AB
42 (0.93)
65
−9 ± 14 


3b{6,4-5}
Allyl
Bu,
 69 ± 11AB
32 (0.89)
40
−1 ± 15 




iPent






3b{6,6}
Allyl
Allyl
 97 ± 3A
20 (0.98)
13
26 ± 16 













3b{5,6}
Allyl
iPent



10 ± 1 3


2b{6}
H
Allyl
 36 ± 10B


5 ± 13













3c{6,1-5}
Allyl
Me, Et, Pr, Bu,
100 ± 0A
 9 (0.99)
7
46 ± 15 




iPent






3c{1,6}
Methyl
Allyl
 62 ± 15AB
30 (0.96)
6
13 ± 13 


3c{2,6}
Ethyl
Allyl
 73 ± 12AB
30 (0.98)
−5
17 ± 14 


3c{3,6}
Propyl
Allyl
100 ± 0A
0.5 (0.98) 
−11
21 ± 14 













3c{5,6}
iPent
Allyl



−25 ± 12 













3c{2,3}
Ethyl
Propyl
100 ± 0A
33.3 (0.99) 
17
10 ± 13 












DEET
not applicable
 60 ± 15AB
47 (0.98) 
7
23 ± 14 






aMe = methyl, Et = ethyl, Pr = propyl, Bu = n-butyl, iPent = isopentyl (= 3-methylbutyl).




bFeeding deterrent effects (Mean ± SE) at 50 μg/cm2are expressed in %. Means followed by the same capitalized letters within a column do not differ significantly (One-way ANOVA. F18.462= 4.4, p < 0.0001; Tukey's test, p < 0.05).




cDC50s (concentrations causing 50% feeding deterrence compared with the control) were calculated for samples showing >50% feeding deterrence in initial screening concentration (50 μg/cm2), using Excel. Linear regression analysis was conducted for all dose-response experimental data. The R2values for the linear regressions are shown in parenthesis after the number.




dMortality and oviposition deterrent (OD) effects were determined at 0.25% for samples showing >50% feeding deterrence in initial screening.



—not tested







Libraries of Ortho Claisen Rearrangement Products from 1-allyloxy-2-alkoxybenzenes


The 5a{1,1-5} mini library had the lowest DC50 value (16 μg/cm2) followed by 5a{2,1-5} and 4a{1-5} (˜20 μg/cm2) (Table 9). A structure-activity relationship was observed among these compounds: small R2 groups (H, methyl or maximally ethyl) gave high feeding deterrence. This activity was lost somewhat with a one or more carbon increase in the size of group R2.


Set 5a{3, 1-5} was the most toxic (Table 9) at 0.25% (47% mortality). A structure-activity relationship could be seen, with R2=propyl being most toxic and R2 groups smaller or larger than that being less toxic. At 0.25% the sets 5a{1,1-5} and 5a{2, 1-5} caused ˜30% oviposition deterrence (Table 9).









TABLE 9







Libraries of ortho Claisen rearrangement products from


1-allyloxy-2-alkoxybenzenes.
























F.D (%)b
DC50c
Mortality
OD (%)d


Compound/

Mean ± SE
μg/cm2
(%)d
Mean ± SE


setsa
R2a
N = 25
(R2) N = 25
N = 3 × 10
N = 35-40















4a{1-5}
H
100 ± 0A
20.6 (0.92)
17
 7 ± 15


5a{1,1-5}
Me
100 ± 0A
15.5 (0.86)
17
32 ± 15


5a{2,1-5)
Et
100 ± 0A
20.0 (0.91)
27
31 ± 15


5a{3,1-5}
Pr
 31 ± 15B

47



5a{4,1-5}
Bu
 6 ± 17B

1



5a{5,1-5}
iPent
 22 ± 16B

7



5a{6,1-5}
allyl
 22 ± 18B

7







aMe = methyl, Et = ethyl, Pr = propyl, Bu = n-butyl, iPent = isopentyl (= 3-methylbutyl). For 5a sets, the code is 5a{R2, R1} (Scheme 1).




bFeeding deterrent effects (Mean ± SE) at 50 μg/cm2are expressed in %. Means followed by the same capitalized letters within a column do not differ significantly (One-way ANOVA, F6.173= 12.5, p < 0.0001; Tukey's test, p < 0.05).




cDC50s (concentrations causing 50% feeding deterrence compared with the control) were calculated for samples showing >50% feeding deterrence in initial screening concentration (50 μg/cm2), using Excel. Linear regression analysis was conducted for all dose-response experimental data. The R2values for the linear regressions are shown in parenthesis after the number.




dMortality and oviposition deterrent (OD) effects were determined at 0.25% for samples showing >50% feeding deterrence in initial screening.



—not tested







Libraries of Ortho Claisen Rearrangement Products from 1-allyloxy-4-alkoxybenzenes


Set 5c{3,1} had the lowest DC50 value (9 μg/cm2) while 4c{1-5} had the highest DC50 value (57 μg/cm2). There was a moderate structure-activity relationship among the sets 5c{R2,1-5}, with aR2=butyl or allyl being less active than R2=methyl, ethyl, propyl or isopentyl. Compounds 5c{3,1} and 5c{1,1} were more active than the entire 5c{3,1-5} or 5c{1,1-5} sets, respectively. Because sets and compounds were tested at the same concentration by weight, this result suggests that the activity detected for the sets came mostly from the most active component. Overall, the structure-activity suggests that good feeding deterrents in the 5c group have an odd-numbered (methyl or propyl) or branched (isopentyl) R2 alkyl group and with a small R1 (methyl) group.


Compound set 6c{1-5} was formed during the synthesis of 5c libraries (in cases when the Claisen reaction was left too long). The cyclic portion of compounds 6c{1-5} resembles a branched chain, and could fit the same type of site as the 5c compounds.


At 0.25% set 5c{1,1} was the most toxic with 38.9% mortality followed by 5c{5,1-5} (Table 10). Other members in the group exhibited <26% mortality (Table 10). Set 5c{2,1-5} demonstrated strong oviposition deterrent activity (63.6%) followed by 4c{1-5} (51.4%) and 5c{1,1-5} (37%). There was some structure-activity relationship with respect to oviposition deterrence, with the optimal R2 alkyl group being ethyl: smaller or larger was less effective. Set 6c{1-5} acted as a moderate oviposition stimulant. Other libraries had modest oviposition deterrent activities (Table 10).









TABLE 10







Libraries of ortho Claisen rearrangement products from 1-allyloxy-4-alkoxybenzenes.
























F.D (%)b
DC50c
Mortality
OD (%)d




Mean ± SE
μg/cm2
(%)d
Mean ± SE


Compound/setsa
R2a
N = 25
(R2) N = 25
N = 3 × 10
N = 35-40















4c{1-5}
H
 51 ± 18ABC
57 (0.92)
3.3
51 ± 17


5c{1,1}
Me
 80 ± 11AB
20 (0.88)
39
14 ± 12


5c{1,1-5}
Me
 68 ± 12 ABC
19 (0.94)
17
37 ± 15


5c{2,1-5}
Et
 66 ± 14ABC
22 (0.95)
3.3
64 ± 17


5c{3,1}
Pr
 92 ± 6A
9.4 (0.90) 
6.4
 7 ± 15


5c{3,1-5}
Pr
 65 ± 11ABC
33 (0.98)
17
21 ± 14












5c{4,1-5}
Bu
 21 ± 16C


−14 ± 13 












5c{5,1-5}
iPent
 83 ± 12AB
15 (0.85)
27
20 ± 15












5c{6,1-5}
allyl
 29 ± 11BC















6c{1-5}
cyclic
100 ± 0A
16 (0.95)
1.0
−12 ± 13 






aMe = methyl, Et = ethyl, Pr = propyl, Bu = n-butyl, iPent = isopentyl (= 3-methylbutyl). For 5c sets, the code is 5c{R2, R1} (Scheme 1).




bFeeding deterrent effects (Mean ± SE) at 50 μg/cm2are expressed in %. Means followed by the same capitalized letters within a column do not differ significantly (One-way ANOVA, F9.241= 4.3, p < 0.0001; Tukey's test, p < 0.05).




cDC50s (concentrations causing 50% feeding deterrence compared with the control) were calculated for samples showing >50% feeding deterrence in initial screening concentration (50 μg/cm2), using Excel. Linear regression analysis was conducted for all dose-response experimental data. The R2values for the linear regressions are shown in parenthesis after the number.




dMortality and oviposition deterrent (OD) effects were determined at 0.25% for samples showing >50% feeding deterrence in initial screening.



—not tested







Libraries of Ortho Claisen Rearrangement Products from 1-allyloxy-3-alkoxybenzenes


Set 5b{5,1}, a mixture of two isomeric compounds (Table 7), exhibited the lowest DC50 value (4 μg/cm2), in one trial. A different lot of set 5b{5,1}exhibited a higher DC50 value (16 μg/cm2). With respect to feeding deterrence there were observed structure-activity relationships. For group R2, propyl gave the best results, and the smaller (methyl, ethyl or allyl) or larger (butyl or isopentyl) groups gave lower feeding deterrence. For group R1 the structure-activity relationship was clear: within each group with R2 constant, there was a decrease in activity in going from R1=methyl to the larger groups. For cases in which isomers x and y were separated, the more compact isomer y was more active as a feeding deterrent than isomer x.


Compound 4b{2-3} was the most toxic, causing 70% mortality at 0.25% (Table 7). Thus group R2═H or methyl gave high mortality. For the larger R2 sets, mortality was lower, and there was a slight pattern with respect to group R1 within each group with constant R2 (ethyl or propyl): the set with R1=ethyl/propyl was more toxic than the set with R1=methyl.


5b{5,1} and 5b{6,1} demonstrated the strongest oviposition deterrent effects (50%). Set 5b{2,4-5} acted as a mild oviposition stimulant (Table 7). There were observed structure-activity patterns in the oviposition data. Among the 4b sets, oviposition deterrence increased with increasing size of group R1. Also in the 5b sets when R2=methyl, ethyl or propyl, there was an increase in oviposition deterrence going from R1=methyl to R1=ethyl/propyl, but when R2=isopentyl or allyl, there was a decrease in oviposition deterrence going from R1=methyl to R1=ethyl/propyl or butyl/isopentyl. In the R2=butyl sets, oviposition deterrence was the same for all R1 groups.


Example 11
Comparison of Toxicity, Oviposition and Feeding Deterrence of Compounds (Group II)

There was no correlation between toxicity and oviposition deterrence (y=−0.0738x+20.582, R2=0.0044) within the data sets. Similarly, there was no correlation between feeding deterrence and oviposition deterrence (y=−0.2005x+25.333, R2=0.0368) within the data sets. Further, there was no correlation between toxicity and feeding deterrence (y=−0.0235x+20.565, R2=0.0006).


Table 11 provides a list of exemplary non-toxic oviposition deterrents.









TABLE 11







Non-toxic oviposition deterrents
















Index =






%
deterrence/


Group
compound
% deterrence
mortality
mortality
Table















II
5c{2,1-5}
64
3
21
10


II
4c {1-5}
51
3
17
10


I
3b{1,1-5}
70
17
4.1
4


II
5b{5,1}
51
16
3.1
7


I
3a{6,1-5}
67
23
2.9
5


II
butyl
34 BL
15
2.2
5



eugenol


II
5c{1,1-5}
37 BL
17
2.1
10


II
5b{6,1}
50
25
2
7


II
3a{3,4}
37 BL
19
1.9
8


II
3a{4,6}
35 BL
19
1.8
8


II
4b{4-5}
45
50
0.9
7


I
3c{4,1-5}
50
58
0.86
3


I
3c{2,2}
75
100
0.75
3


I
3c{2,1-5}
57
97
0.58
3









Table 12 provides a list of exemplary non-toxic feeding deterrents.









TABLE 12







Non-toxic feeding deterrents

















Index =







FD/(DC50 ×


Group
compound
% deterrence
DC50
% mortality
mortality)















II
3c{3,6}
100
0.5
−11b 
2000


II
5b{3,1}y
100
14

  −4.2b

71


II
5b{6,1}
92
23

−2b

40


II
5b{3,2}x
95
25
0b
38


II
5b{3,2}
92
17
  0.6
9


II
5b{3,2}
92
17
  0.6
9


II
6c{1-5}
100
16
 1
6.3


II
5b{5,1}
99
4
 6
4


II
5c{3,1}
92
9
 6
1.7


II
3c{6,1-5}
100
9
 7
1.6


II
5b{3,1}
100
16
 4
1.6


II
5b{2,1}
93
24
 4
1


II
5b{3,2-3}
93
9
10
1


I
3c{5,1-5}
100
6
19
0.9


I
3a{4,1-5}
98
17
 7
0.8


II
3b{3,5}
97
17
10
0.6


II
3a{3,6}
96
12-16a
10-17a
0.5


II
5b{2,4-5}
81
15
10
0.5


I
3a{3,1-5}
100
19
14
0.4


II
3b{6,6}
97
20
13
0.4


II
5a{1,1-5}
100
16
17
0.4


II
5b{3,1}
91
26
10
0.35


II
3a{4,6}
92
17
19
0.3


II
4a{1-5}
100
21
17
0.3


II
allyl
86
15
18
0.3


I
3c{6,6}
97
24
16
0.25


II
3a{3,4}
98
21
19
0.25


I
3c{3,3}
96
20
23
0.2


I
3b{4,1-5}
84
14
30
0.2


I
3b{5,1-5}
83
20
21
0.2


II
5c{5,1-5}
83
15
27
0.2


II
5b{6,2-3}
90
16
33
0.2


II
5a{2,1-5}
100
20
27
0.19


II
3c{2,3}
100
33
17
0.18


I
3c{1,1-5}
80
35
23
0.1


I
3c{4,1-5}
83
15
58
0.1


II
3b{1.5}
89
29
30
0.1


II
5c{1,1}
80
20
39
0.1


I
3b{3,1-5}
98
22
50
0.09


I
3b{2,2}
89
29
37
0.08


I
3b{3,3}
97
27
77
0.05


I
3c{2,1-5}
91
23
97
0.04


I
3c{2,2}
81
26
100 
0.03






aUsed the average




bUsed 0.1, in order to get meaningful values







Example 12
Comparison of Feeding Deterrence with Botanical Insecticides

Based upon antifeedant activity, the compounds/libraries of possess levels of activity that compare to some of the most active botanical insecticides in current use. One of the compounds, 3c{3,6} in the group is more active than pyrethrum (DC50=0.9 μg/cm2) on third instar T. ni larvae using the feeding deterrent bioassay. Similarly, other compounds/libraries including 5c{3,1}, 3c{6,1-5}, 5b{3,2-3} and 5b{5,1} x+y were more active than rotenone against third instar, T. ni larvae. All of the compounds/libraries were more active than rosemary oil (DC50 value of 158 μg/cm2), clove leaf oil (DC50 value of 217 μg/cm2), Melia azedarach (DC50=288 μg/cm2), Trichilia americana (DC50=190 μg/cm2) and ryania (DC50=725 μg/cm2) (Akhtar et al. 2008).


Example 13
Comparison of Compounds (Group II) with DEET

Many of the pure compounds and libraries were more active than a commercial insect repellent, DEET, as feeding and/or oviposition deterrents against T. ni larvae and adult female moths, respectively. DEET exhibited the highest DC50 value (47 μg/cm2) in the whole group (Table 8) as opposed to the low DC50 value of 0.5 μg/cm2 exhibited by 3c{3,6}.


Example 14
Test of Compound 3c{3,6} on T. ni Neonates in Greenhouse

The compound 1-allyloxy-4-propoxybenzene, 3c{3,6}, was tested in the Greenhouse at 3 different concentrations (2 μg, 5 μg and 10 μg). Concentrations in % are as follows: 0.0002%, 0.005% and 0.01%. Cabbage plants had 3-5 leaves. Each plant was sprayed (˜1 ml/leaf) and dried before the introduction of insects. There were 3 replicates of 5 plants for each treatment. Approximately nine T. ni neonates (<24 h old) were transferred on each plant. They were weighed after 6 days of feeding on the treatments. Numbers of larvae recovered from each treatment were also recorded. The results indicate that weight of the larvae was not affected at the lowest concentration (0.0002%), but that the weight reduction was 33.3% and 48% at 0.0005 and 0.001%, respectively (FIG. 3). This suggests that larvae are feeding less and, therefore, gaining less weight. The mean larval recovery was not affected at the lowest concentration (0.0002%). However, mean larval recovery was 66.7% and 52% at 0.0005 and 0.001%, respectively (FIG. 4). This suggests that some of the larvae were not surviving.


The highest concentration of the compound used in the experiment was 10 ppm. A growth reduction of ˜50% and larval recovery was observed at this concentration. For comparison, the amount of active ingredient in most of the commercial insecticides varies from 24-24000 ppm.


Table 13 provides a list of the test compounds of Group I.









TABLE 13







Test Compounds Group I











Table Reference
Compound name (IUPAC)
R1
R2
Name





Table 3
1,4-dimethoxybenzene
methyl
methyl
3c{1,1}



1,4-diethoxybenzene
ethyl
ethyl
3c{2,2}



1,4-dipropoxybenzene
propyl
propyl
3c{3,3}



1,4-diisopentoxybenzene
isopentyl
isopentyl
3c{5,5}



1,4-diallyloxybenzene
allyl
allyl
3c{6,6}



Me library (1-methoxy-4-
methyl
methyl,ethyl,propyl,isopentyl
3c{1,1-



alkoxybenzene)


5}



Et library (1-ethoxy-4-
ethyl
methyl,ethyl,propyl,isopentyl
3c{2,1-



alkoxybenzene)


5}



Pr library (1-propoxy-4-
propyl
methyl,ethyl,propyl,isopentyl
3c{3,1-



alkoxybenzene)


5}



Bu library (1-butoxy-4-
butyl
methyl,ethyl,propyl,isopentyl
3c{4,1-



alkoxybenzene)


5}



iPent library (1-isopentyloxy-4-
isopentyl
methyl,ethyl,propyl,isopentyl
3c{5,1-



alkoxybenzene)


5}



allyl small library (1-allyloxy-4-
allyl
methyl,ethyl,propyl
3c{6,1-



alkoxybenzene)


3}



1-allyloxy-4-butoxybenzene
allyl
butyl
3c{4,6}



1-allyloxy-4-isopetoxybenzene
allyl
isopentyl
3c{5,6}



1-hydroxy-4-methoxybeznene
H
methyl
2c{1}



1-hydroxy-4-ethoxybeznene
H
ethyl
2c{2}



1-hydroxy-4-propoxybenzene
H
propyl
2c{3}



1-hydroxy-4-isopentoxybenzene
H
isopentyl
2c{5}


Table 4
1,3-dimethoxybenzene
methyl
methyl
3b{1,1}



1,3-diethoxybenzene
ethyl
ethyl
3b{2,2}



1,3-dipropoxybenzene
propyl
propyl
3b{3,3}



1,3-diisopentoxybenzene
isopentyl
isopentyl
3b{5,5}



Me library (1-methoxy-3-
methyl
methyl,ethyl,propyl,isopentyl
3b{1,1-



alkoxybenzene)


5}



Et library (1-ethoxy-3-
ethyl
methyl,ethyl,propyl,isopentyl
3b{2,1-



alkoxybenzene)


5}



Pr library (1-propoxy-3-
propyl
methyl,ethyl,propyl,isopentyl
3b{3,1-



alkoxybenzene)


5}



Bu library (1-butoxy-3-
butyl
methyl,ethyl,propyl,isopentyl
3b{4,1-



alkoxybenzene)


5}



iPent library (1-isopentyloxy-3-
isopentyl
methyl,ethyl,propyl,isopentyl
3b{5,1-



alkoxybenzene)


5}



1-hydroxy-3-methoxybenzene
H
methyl
2b{1}



1-hydroxy-3-ethoxybeznene
H
ethyl
2b{2}



1-hydroxy-3-propoxybenzene
H
propyl
2b{3}



1-hydroxy-3-isopentoxybenzene
H
isopentyl
2b{5}


Table 5
1,2-dimethoxybenzene
methyl
methyl
3a{1,1}



1,2-diethoxybenzene
ethyl
ethyl
3a{2,2}



1,2-dipropoxybenzene
propyl
propyl
3a{3,3}



1,2-dibutoxybenzene
butyl
butyl
3a{4,4}



1,2-diisopentoxybenzene
isopentyl
isopentyl
3a{5,5}



1,2-diallyloxybenzene
allyl
allyl
3a{6,6}



Me library (1-methoxy-2-
methyl
methyl,ethyl,propyl,butyl,isopentyl
3a{1,1-



alkoxybenzene)


5}



Et library (1-ethoxy-2-
ethyl
methyl,ethyl,propyl,butyl,isopentyl
3a{2,1-



alkoxybenzene)


5}



Pr library (1-propoxy-2-
propyl
methyl,ethyl,propyl,butyl,isopentyl
3a{3,1-



alkoxybenzene)


5}



Bu library (1-butoxy-2-
butyl
methyl,ethyl,propyl,butyl,isopentyl
3a{4,1-



alkoxybenzene)


5}



iPent library (1-isopentyloxy-2-
isopentyl
methyl,ethyl,propyl,butyl,isopentyl
3a{5,1-



alkoxybenzene)


5}



allyl library (1-allyloxy-2-
allyl
methyl,ethyl,propyl,butyl,isopentyl
3a{6,1-



alkoxybenzene)


5}



1-hydroxy-2-allyloxybenzene
H
allyl
2a{6}



1-hydroxy-2-methoxybenzene
H
methyl
2a{1}



1-hydroxy-2-ethoxybeznene
H
ethyl
2a{2}



1-hydroxy-2-propoxybenzene
H
propyl
2a{3}



1-hydroxy-2-butoxybenzene
H
butyl
2a{4}


Table 6
1,2-diethoxybenzene
ethyl
ethyl
3c{2,2}


(list of most
1,3-dipropoxybenzene
propyl
propyl
3b{3,3}


active
1-ethoxy-4-alkoxybenzene
ethyl
methyl,ethyl,propyl,isopentyl
3c{2,1-


compounds,



5}


listed in order of
1-butoxy-4-alkoxybenzene
butyl
methyl,ethyl,propyl,isopentyl
3c{4,1-


the table, from



5}


top left across
1-propoxy-3-alkoxybenzene
propyl
methyl,ethyl,propyl,isopentyl
3b{3,1-


the first row,



5}


then across the
1,4-dipropoxybenzene
propyl
propyl
3c{3,3}


second row and
1,4-diallyloxybenzene
allyl
allyl
3c{6,6}


across the third
1-allyloxy-4-butoxybenzene
allyl
butyl
3c{4,6}


row)
allyl small library (1-allyloxy-4-
allyl
methyl,ethyl,propyl
3c{6,1-



alkoxybenzene)


3}



iPent library (1-isopentyloxy-4-
isopentyl
methyl,ethyl,propyl,isopentyl
3c{5,1-



alkoxybenzene)


5}



iPent library (1-isopentyloxy-3-
isopentyl
methyl,ethyl,propyl,isopentyl
3b{5,1-



alkoxybenzene)


5}



Pr library (1-propoxy-2-
propyl
methyl,ethyl,propyl,butyl,isopentyl
3a{3,1-



alkoxybenzene)


5}



Bu library (1-butoxy-2-
butyl
methyl,ethyl,propyl,butyl,isopentyl
3a{4,1-



alkoxybenzene)


5}



allyl library (1-allyloxy-2-
allyl
methyl,ethyl,propyl,butyl,isopentyl
3a{6,1-



alkoxybenzene)


5}









Table 14 provides a list of the test compounds of Group II.









TABLE 14







Test compounds — Group II











Table Reference
Compound name (IUPAC)
R1
R2
Name





Table 8
1-allyloxy-2-propoxybenzne
Allyl
Propyl
3a{3,6}






3a{3,6}



1-allyloxy-2-butoxybenzene
Allyl
Butyl
3a{4,6}



1-butoxy-3-propoxybenzene
Propyl
Butyl
3a{3,4}



allyl library (1-allyloxy-
Allyl
Me, Et, Pr,
3a{6,1-5}



2-alkoxybenzene)

Bu, iPent




1-allyloxy-2-
Allyl
iPent
3a{5,6}



isopentoxybenzene






1-propoxy-3-
Propyl
iPent
3b{3,5}



isopentoxybenzene






1-methoxy-3-
Methyl
iPent
3b{1,5}



isopentoxybenzene






1-allyloxy-3-methoxybenzene
Methyl
Allyl
3b{1,6}



small allyl set (1-allyloxy-
Allyl
Et, Pr
3b{6,2-3}



3-ethoxy/propoxybenzene)






small allyl set (1-allyloxy-
Allyl
Bu, iPent
3b{6,4-5}



3-butoxy/isopentoxybenzene)






1,3-diallyloxybenzene
Allyl
Allyl
3b{6,6}



1-allyloxy-3-
Allyl
iPent
3b{5,6}



isopentoxybenzene






1-hydroxy-2-allyloxybenzne
H
Allyl
2b{6}



allyl library (1-allyloxy-
Allyl
Me, Et,
3c{6,1-5}



4-alkoxybenzene)

Pr, Bu, iPent




1-allyloxy-4-methoxybenzene
Methyl
Allyl
3c{1,6}



1-allyloxy-4-ethoxybenzene
Ethyl
Allyl
3c{2,6}



1-allyloxy-4-propoxybenzene
Propyl
Allyl
3c{3,6}



1-allyloxy-4-
iPent
Allyl
3c{5,6}



isopentoxybenzene






1-ethoxy-4-propoxybenzene
Ethyl
Propyl
3c{2,3}





Table 9
R3 = allyl (1-alkoxy-2-(hydroxy or alkoxy)-3-allylbenzene)
Me, Et, Pr, Bu, iPent
H
4a{1-5}














Me, Et,
Me
5a{1,1-5}



Pr, Bu,





iPent





Me, Et,
Pr
5a{3,1-5}



Pr, Bu,





iPent





Me, Et,
Bu
5a{4,1-5}



Pr, Bu,





iPent





Me, Et,
iPent
5a{5,1-5}



Pr, Bu,





iPent





Me, Et,
allyl
5a{6,1-5}



Pr, Bu,





iPent













Table 10
R3 = allyl (1-(hydroxy or alkoxy)- 2-allyl- 4-alkoxybenzene)
Me, Et, Pr, Bu, iPent
H
4c{1-5}














Me
Me
5c{1,1}



Me, Et,
Me
5c{1,1-5}



Pr, Bu,





iPent





Me, Et,
Et
5c{2,1-5}



Pr, Bu,





iPent





Pr
Pr
5c{3,1}



Me, Et,
Pr
5c{3,1-5}



Pr, Bu,





iPent





Me, Et,
Bu
5c{4,1-5}



Pr, Bu,





iPent





Me, Et,
iPent
5c{5,1-5}



Pr, Bu,





iPent





Me, Et,
allyl
5c{6,1-5}



Pr, Bu,





iPent





















R4; dihydrofuran
Me, Et, Pr, Bu, iPent
cyclic (dihydrofuran)
6c{1-5}





Table 7

Me
H
4b{1}




Et, Pr
H
4b{2-3}




Bu, iPent
H
4b{4-5}



R3 = allyl
Me
Me
5b{1,1}










isomer x: 1-allyl-2-alkoxy- 4-alkoxybenzene isomer y: 1-alkoxy-2-allyl- 3-alkoxybenzene
Et, Pr
Me
5b{1,2-3}














Bu, iPent
Me
5b{1,4-5}



Me
Et
5b{2,1}



Et, Pr
Et
5b{2,2-3}



Bu, iPent
Et
5b{2,4-5}



Me
Pr
5b{3,1}



Et, Pr
Pr
5b{3,2-3}



Bu, iPent
Pr
5b{3,4-5}



Et
Pr
5b{3,2}



Me
Bu
5b{4,1}



Et, Pr
Bu
5b{4,2-3}



Bu, iPent
Bu
5b{4,4-5}



Me
iPent
5b{5,1}



Et, Pr
iPent
5b{5,2-3}



Bu, iPent
iPent
5b{5,4-5}



Me
allyl
5b{6,1}



Et, Pr
allyl
5b{6,2-3}



Bu, iPent
allyl
5b{6,4-5}



Me
Me
5b{1,1}



Me
Pr
5b{3,1}



Me
Pr
5b{3,1} y





(100% y)





5b{3,1} x





(68% x,





32% y)



Et
Pr
5b{3,2}



Et
Pr
5b{3,2} y





(100% y)



Et
Pr
5b{3,2} y





(82% x,





18% y)



Me
iPent
5b{5,1}



Me
allyl
5b{6,1}









OTHER EMBODIMENTS

Although various embodiments of the invention are disclosed herein, many adaptations and modifications may be made within the spirit and scope of the invention in accordance with the common general knowledge of those skilled in this art. Such modifications include the substitution of known equivalents for any aspect of the invention in order to achieve the same result in substantially the same way. Numeric ranges are inclusive of the numbers defining the range, and of sub-ranges encompassed therein. As used herein, the terms “comprising”, “comprises”, “having” or “has” are used as an open-ended terms, substantially equivalent to the phrase “including, but not limited to”. Terms such as “the,” “a,” and “an” are to be construed as indicating either the singular or plural. Citation of references herein shall not be construed as an admission that such references are prior art to the present invention. All publications are incorporated herein by reference as if each individual publication were specifically and individually indicated to be incorporated by reference herein and as though fully set forth herein. The invention includes all embodiments and variations substantially as hereinbefore described and with reference to the examples and drawings.


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Claims
  • 1. A method for controlling infestation by a Trichoplusia ni comprising applying an effective amount of a compound of Formula I:
  • 2. The method of claim 1 wherein the controlling is selected from the group consisting of one or more of comprises oviposition deterrence, feeding deterrence, oviposition stimulation, feeding stimulation, and toxicity.
  • 3. The method of claim 1 wherein the compound of Formula I is an oviposition deterrent.
  • 4. The method of claim 3 wherein the compound of Formula I is selected from the group consisting of one or more of 3a{4,6}, 3a{3,4}, 5c{1,1-5}, 4b{4-5}, 3c{4,1-5}, 5b{6,1}, 4c {1-5}, 5b{5,1}, 3c{2,1-5}, 5c{2,1-5}, 3a{6,1-5}, 3a{6,1-5}, 3b{1,1-5}, or 3c{2,2}.
  • 5. The method of claim 1 wherein the compound of Formula I is a feeding deterrent.
  • 6. The method of claim 5 wherein the compound of Formula I is selected from the group consisting of one or more of 3c{1,1-5}, 5c{1,1}, 3c{2,2}, 5b{2,4-5}, 3c{4,1-5}, 3b{5,1-5}, 5c{5,1-5}, 3b{4,1-5}, 3b{2,2}, 3b{1.5}, 5b{6,2-3}, 3c{2,1-5}, 5b{3,1}, 3a{4,6}, 5c{3,1}, 5b{3,2}, 5b{3,2}, 5b{6,1}, 5b{2,1}, 5b{3,2-3}, 5b{3,2}x, 3c{3,3}, 3a{3,6}, 3c{6,6}, 3b{3,3}, 3b{3,5}, 3b{6,6}, 3b{3,1-5}, 3a{4,1-5}, 3a{3,4}, 5b{5,1}, 3c{5,1-5}, 3a{3,1-5}, 3c{6,1-5}, 3c{3,6}, 3c{2,3}, 4a{1-5}, 5a{1,1-5}, 5a{2,1-5}, 6c{1-5}, 5b{3,1}, or 5b{3,1}y.
  • 7. The method of claim 1 wherein the compound of Formula I is an oviposition stimulant.
  • 8. The method of claim 7 wherein the compound of Formula I is selected from the group consisting of one or more of 3c{5,6} and 5b{2,4-5}.
  • 9. The method of claim 1 wherein the compound of Formula I is a feeding stimulant.
  • 10. The method of claim 9 wherein the compound of Formula I is selected from the group consisting of one or more of 2b{2}, 2c{1}, and 2c{3}.
  • 11. The method of claim 1 wherein the compound of Formula I is toxic or is non-toxic.
  • 12. The method of claim 11 wherein the toxicity is selective for T. ni.
  • 13. The method of claim 1 wherein two or more compounds of Formula I are applied simultaneously or sequentially.
  • 14. The method of claim 1 wherein the compound of Formula I is applied in combination with another compound or treatment.
  • 15. The method of claim 14 wherein the other compound is selected from one or more of the group consisting of an oviposition deterrent, an oviposition stimulant, a feeding deterrent, a feeding stimulant, an attractant, and a toxicant.
  • 16. The method of claim 1 wherein the T. ni is a larva or an adult.
  • 17. The method of claim 1 wherein the site of interest comprises a plant or part thereof.
  • 18. The method of claim 1 wherein the compound of Formula I is provided in a formulation selected from one or more of the group consisting of a spray, aerosol, solid, or liquid.
  • 19. A method of protecting a plant from infestation by a Trichoplusia ni comprising applying an effective amount of a compound of Formula I:
  • 20. A composition comprising one or more compounds selected from one or more of an oviposition deterrent, an oviposition stimulant, a feeding deterrent, a feeding stimulant and toxicant.
Parent Case Info

This application claims the priority benefit of U.S. Provisional application 61/116,235, filed Nov. 19, 2008, the contents of which is herein incorporated by reference.

Provisional Applications (1)
Number Date Country
61116235 Nov 2008 US