Claims
- 1. An isolated polynucleotide comprised of a sequence encoding a mycobacterial InhA polypeptide, wherein the polypeptide has at least 80% sequence identity to the mycobacterial polypeptide encoded in SEQ ID NO:1 and is involved in mycolic acid biosynthesis.
- 2. The isolated polynucleotide of claim 1, wherein the polypeptide can confer isoniazid (INH) sensitivity to mycobacterium.
- 3. The isolated polynucleotide of claim 1, wherein the polypeptide can confer INH resistance to mycobacterium.
- 4. An isolated polynucleotide comprised of a sequence encoding a mycobacterial InhA polypeptide, wherein the polypeptide has at least 80% sequence identity to the mycobacterial polypeptide encoded in SEQ ID NO:3 and is involved in mycolic acid biosynthesis.
- 5. The isolated polynucleotide of claim 4, wherein the polypeptide can confer INH sensitivity to mycobacterium.
- 6. The isolated polynucleotide of claim 4, wherein the polypeptide can confer INH resistance to mycobacterium.
- 7. An isolated polynucleotide according to claim 1, wherein the polypeptide has at least 90% sequence identity to the mycobacterial polypeptide encoded in SEQ ID NO:1.
- 8. The isolated polynucleotide of claim 7, wherein the polypeptide can confer INH sensitivity to mycobacterium.
- 9. The isolated polynucleotide of claim 7, wherein the polypeptide can confer INH resistance to mycobacterium.
- 10. An isolated polynucleotide according to claim 1, wherein the InhA polypeptide has the amino acid sequence present in that of SEQ ID NO:4.
- 11. An isolated polynucleotide according to claim 1, wherein the InhA polypeptide has the amino acid sequence present in that of SEQ ID NO:5.
- 12. An isolated polynucleotide according to claim 1, wherein the InhA polypeptide has the amino acid sequence present in that of SEQ ID NO:6.
- 13. An isolated polynucleotide according to claim 1, wherein the InhA polypeptide has the amino acid sequence present in that of SEQ ID NO:7.
CROSS-REFERENCE TO RELATED APPLICATIONS
This is a Continuation-in-Part of application Ser. No. 08/062,409 filed May 14, 1993 now abandoned entitled USE OF GENES OF M. TUBERCULOSIS AND M. SMEGMATIS WHICH CONFER ISONIAZID RESISTANCE TO TREAT TUBERCULOSIS AND TO ASSESS DRUG RESISTANCE, which is hereby incorporated by reference herein in its entirety.
STATEMENT OF GOVERNMENT INTEREST
This invention was made with government support under NIH Grant No. A126170 and National Cooperative Drug Discovery Group Grant No. UO1A130189. As such, the United States government has certain rights in the invention.
US Referenced Citations (1)
Number |
Name |
Date |
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5573915 |
Barry |
Nov 1996 |
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Non-Patent Literature Citations (5)
Entry |
Banerjee et al., "inhA, a gene encoding a target for isoniazid and ethionde in Mycobacterium tuberculosis" Science (1994) 263:277-230. |
Uhlmann et al., "Antisense oligonucleotides: A new therapeutic principle" Chem. Reviews (1990) 90(4):543-584. |
Orita et al., "Rapid and sensitive detection of point mutations and DNA polymorphisms using the polymerase chain reaction" Genomics (1989) 5:874-879. |
Telenti et al., "Rapid identification of Mycobacteria to the species level by polymerase chain reaction and restriction enzyme analysis" J. Clin. Microbiol. (1993) 31(2):175-178. |
Zhang et al Nature 358 591-593, 1992. |
Continuation in Parts (1)
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Number |
Date |
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Parent |
62409 |
May 1993 |
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