Patent Application
20100112547
The Sequence Listing provided in file 020054—004146US_SEQLIST.txt, of 295,121 bytes in size and was created on Aug. 11, 2009, is hereby incorporated by reference in its entirety for all purposes.
Epidemic viral infections are responsible for significant worldwide loss of life and income in human illnesses ranging from the common cold to life-threatening influenza, West Nile and HIV infections. Timely detection, diagnosis and treatment are key in limiting spread of disease in epidemic, pandemic and epizootic settings. Rapid screening and diagnostic methods are particularly useful in reducing patient suffering and population risk. Similarly, therapeutic agents that rapidly inhibit viral assembly and propagation are particularly useful in treatment regimens.
Influenza A has emerged recently as a potential significant risk to human populations. Avian strains have crossed into humans and there is growing evidence that human to human spread may soon occur1. Examples of the impact of avian influenza strains on human populations is provided by the recent emergence of highly virulent strains of avian influenza H5N1 (bird flu) where approximately 50% of infected individuals (42 people) succumbed and food shortages resulted from slaughter of millions of birds in China, Indonesia and Vietnam. Tracking the potential for epidemic, the World Health Organization considered raising the global threat level to 4 or 5 (on a scale of six) in July of 2005. One opinion leader recently expressed in press that with avian influenza—“detection, surveillance, prevention and therapy” . . . (is) . . . “a race against time”1. Since avian strains have rarely been isolated from humans and mortality rates in humans are high, it seems likely that immunity in the worldwide population is virtually non-existent. Thus, the opportunity exists for a worldwide pandemic. For comparison, in 1918 a global influenza epidemic resulted in an estimated 20-40 million deaths. With increased population density today, higher mortality is likely.
Virology test methods for detection and confirmation of influenza A infection in a virus-secure reference laboratory, e.g., satisfying requirements for Containment Group 4 pathogens, are time consuming, high-risk and laborious, i.e., involving 4-7 days isolation of the virus in embryonated eggs; harvesting allantoic fluids from dead or dying embryos; testing the fluid in hemagglutination and hemagglutination inhibition tests, immunodiffusion; and, eventual subtyping of the virus in the fluid by hemagglutinin and neuraminidase in overnight immunodiffusion assays using specially prepared monospecific antisera. Present subtyping involves identifying each of 16 different possible viral hemagglutinin proteins in combination with 9 different possible viral neuraminidase proteins. Unfortunately, since only a few pathogenic strains of influenza A are of economic and health concern at any point in time, much of this time-consuming effort may be unnecessary and wasted.
Current rapid immunodiagnostic tests for influenza antigens like “Binax NOW FluA and FluB™” (Binax, Inc., Portland, Me.), “Directigen Flu A+B™” (Becton Dickinson, Franklin Lakes, N.J.), “Flu OIA™” (Biostar Inc., Boulder, Colo.), “Quick Vue™” (Quidel, Sand Diego, Calif.), “Influ AB Quick™” (Denka Sieken Co., Ltd., Japan) and “Xpect Flu A & B” (Remel Inc., Lenexa, Kans.), can reportedly either detect influenza A or distinguish between Influenza A and B, but importantly, not between different influenza A subtypes or between pathogenic and non-pathogenic strains of influenza A. The complexity of the test formats may require special training. In addition, significant amounts of virion particles are commonly required to obtain a positive test result, limiting their use to a short window of time when virus shedding is at its highest levels. Assay sensitivity is also variable with up to 20% false negative test results in certain assays being of significant current concern (e.g., see “WHO recommendations on the use of rapid testing for influenza diagnosis”, July 2005). Recent introduction of reverse-transcriptase PCR-based diagnostics (RT-PCR) for confirming influenza A virus have resulted in important advances in capabilities36, but are laborious and require highly trained personnel making on-site or field-testing difficult. Because of the relative inefficiency of the reverse transcriptase enzyme, significant amounts of virus (e.g., 104 virion particles) and as many as 20 primers may be required to effectively detect viral RNA. Despite these significant obstacles, in reference laboratory RT-PCR influenza A testing high levels of proficiency have recently been recorded between 12 different participating test laboratories in the US, Canada and Hong Kong36. Using RT-PCR and HA primers, Lee et al.37 described quantitative discrimination between H5 and H7 subtypes of virus. Munch et al.38 report similar possible differential specificity in RT-PCR using NP primers. Unfortunately, RT-PCR is not easily adapted to high throughput screening of subjects in an epidemic setting or to field uses in an agricultural or point-of-care setting.
Additionally, the complexity, diversity and rapid emergence of new influenza strains has made diagnosis of high risk strains difficult, and therefore rapid response is at present nearly impossible. For epidemiologists, diversity resulting from high mutation rates and genetic reassortment make it difficult to anticipate where new strains may originate and respond with the timely introduction of new diagnostic primers for PCR. As a result, (at present) the diversity of influenza dictates the necessity of multiplex PCR approaches.
Avian influenza virus (H5N1) is believed to be evolving by both mutation and segmental reassortment with influenza viruses in aquatic wildfowl2,3. Highly pathogenic disease in “sick” birds may vary from sudden death with few overt signs of disease to a more characteristic disease with respiratory signs, excessive lacrimation, sinusitis, edema of the head, cyanosis of the unfeathered skin and diarrhea, i.e., the diagnostic signs of “sick” employed by OIE in their health guidelines (Manual of Diagnostic Tests and Vaccines for Terrestrial Animals, 5th edition, 2004, World Organization for Animal Health). In infected birds influenza A virus is shed in just 2-3 days4,5. Given the high mortality rates in humans, rapid detection is essential to isolate infected avian and human subjects and protect human populations. In the field, human cases of bird flu have historically originated in regions of South East Asia that lacks easy access to sophisticated diagnostic test equipment, virus-secure reference BL4 laboratories and methods. Thus, assessing population risk at an individual patient level is presently highly problematic. In other objects, the invention offers solutions to these problems.
Rapid diagnostic testing, needed to support agriculture and the public health, is proving to be challenging—i.e., for either serological detection of anti-viral host responses (antibody) or identification of viral proteins (antigens) in samples. Testing for influenza A subtypes is also complicated by: (i) the scope of epidemiological and the public health needs, i.e., potential needs for viral detection in environmental samples and in infected livestock, (e.g. swine flu), poultry (e.g. avian flu) and humans (e.g. bird flu); and, (ii) the wide range of possible test samples which may include serum, nasopharyngeal, throat gargle, nasal or laryngeal samples (human); and, cloacal, feces and tracheal samples (bird). Since high risk viruses tend to spread rapidly, speed is of the essence. High affinity specific binding reagents are clearly key and required. In other objects, the invention solves these key needs.
Classical influenza serological testing (for antibody) by hemagglutination-inhibition (HI) is relatively simple, but in agricultural practice these tests are relatively insensitive for detecting avian antibody responses following either vaccination or natural infection as serum antibody tends to fall rapidly after infection. Under optimal conditions Xu et al.39 e.g. recently described a latex agglutination test, i.e., using complete heat inactivated vaccine virus and serum from vaccinated birds. The latter HI-test reportedly had 88% sensitivity (12% false negatives) and 98% specificity, in this case, false negative rates too high for agricultural or public health detection of such dangerous viral pathogens. Similarly, using avian field samples in China, Jin et al.4 recently described potential uses of a recombinant influenza NP antigen in ELISA assays. These investigators observed that virus shedding began at days 2, but titers of anti-viral antibodies were most significant at 2 weeks. Unfortunately, the latter “lag” before detection of infected animals is unacceptable in the current worldwide crisis. Demonstrating a further possible complication, data in the latter studies showed that low doses of virus generated only very low titers of antibody, i.e., suggesting that subclinical infections might go undetected.
Present limitations in routine diagnostic methods for flu, i.e., Influenza B, were noted in data published by Steininger et al.41. In the latter studies, different test methods were employed to detect a standard influenza A stock virus preparation; and, with the following findings: namely, rapid enzyme-based assays were about 1000-fold less sensitive than detection by conventional virus isolation methods; which were, in turn, about 1000-fold less sensitive than RT-PCR. Despite the latter gross quantitative limitations in sensitivity, the ELISA still correctly identified 62% of positive samples and 88% for samples obtained from patients younger than 5 yrs. of age with Influenza B (flu). As an example of the impact that poor samples can have on assay performance, commercially viable flu tests were assayed for their sensitivity in detecting viral antigen in nasopharyngeal samples of experimentally infected volunteers. The reported results suggest that assay sensitivity was about 60% for the Directigen flu test43 (Becton Dickinson); and, in the range of 48-100% for the flu optical immunoassay (FLU OIA; ThermoBioStar/Biota)44. Importantly, (despite the obvious limitations of the latter tests), Sharma et al.45 reported that rapid confirmation of influenza virus type A infection: (i) decreased irrelevant laboratory testing, e.g. urinalysis and wbc testing, as well as, (ii) inappropriate antibiotic use in febrile infants and toddlers. Thus, a relatively poor sensitivity in these screening assays was still useful in clinical practice because the assay correctly identified those patients who needed additional follow-up. Clearly, for non-reference lab uses, improvements in user friendliness, speed, discrimination and absolute quantitative sensitivity are needed, i.e., even for routine flu testing. Similarly, routine flu testing is not particularly helpful in suggesting how one may achieve a method with the requisite assay performance needed to test for high risk strains of influenza A in patient samples.
Emergent virulence factors in H5N1 and H7 avian influenza A viruses and the panzooic spread of H9N2 influenza virus and their known interactions with mammalian host factors have been reviewed5. Among the proteins encoded by virulent avian strains of influenza, NS1 (non-structural protein-1) is expressed early in infected cells, but unlike HA and NA, it is not virion associated and is expressed only as an intracellular protein. NS1 is encoded by genome segment 8 and is a viral regulatory factor enhancing translation of viral mRNA; interfering with maturation and transport of host cell mRNA6; binding poly(A) tails of host mRNA; altering intrinsic small interfering RNA (siRNA) control of host cell gene expression7; preventing ds-RNA induction of antiviral protein kinase R; inhibiting induction8 of, and antagonizing9,10 anti-viral action of interferon α/β (IFN-α/β); and, stimulating production of pro-inflammatory cytokines by macrophages11 and dendritic cells12. The roles of INF-α/β signaling in innate and adaptive immune responses and pathogenesis has recently been reviewed.13
Distribution of NS1 protein in infected cells suggest preferential nuclear localization, i.e., but with lesser amounts in cytoplasmic, ribosomal and polysomal fractions22-24. NS1 protein of the highly virulent avian H5N1 strain apparently suppresses interferon responses of human cells in vitro25. Certain mechanistic studies suggest that carboxyl terminal deletions in NS1, may attenuate in vivo virulence of wild-type A/Swine/Texas/4199-2/98 (TX/98) virus26, as well as, equine influenza virus27. Interestingly, Influenza A lacking the NS1 gene seems to replicates best in interferon-deficient cell lines28, suggesting to the authors that NS1 inhibition of INF-α/β may be necessary for efficient viral propagation. In addition, reassortment of the high-virulence H5N1-NS1 gene into the lower virulence H1N1-A strain reportedly reduced lung clearance rates of the hybrid virus, and also resulted in increased levels of inflammatory cytokines29. Tumpey et al.40 reported that detecting anti-NS1 antibodies may be useful in distinguishing vaccinated from infected poultry, i.e., because NS1 is only expressed in infected cells not in inactivated gradient purified vaccine virus. Unfortunately, the latter antibody-based serological test methods suffer from the same general problems identified above in regard to HI tests: namely, low sensitivity and inability to detect virus prior to virus shedding and potential spread of infection.
Using the H7N3 strain, Cattoli et al.42 reportedly evaluated the timing, specificity and sensitivity of detection of virus in tracheal samples from experimentally and naturally infected turkeys, i.e., in antigen-capture ELISA, RT-PCR and a real-time RT-PCR, (i.e., the later two tests targeting the M gene). Under the latter relatively controlled laboratory conditions, virus was detectable with good specificity and sensitivity as early as 3-5 days post-infection. They concluded that it should be theoretically possible to detect, at least this particular avian virus and perhaps other more highly virulent avian strains at day 3 to 5 of infection provided there were sufficient assay sensitivity.
Thus, there remains a significant need in the medical arts for improved, inexpensive, rapid, accurate and discriminatory methods capable of detecting the particular strains of pathogenic viruses most often involved in generating medically important diseases. There is also a special need for simple assay methodologies that can be routinely used by relatively untrained individuals in underdeveloped nations, markets, clinics, doctor's and veterinary offices, schools and food processing plants where resources may be limited and sophisticated lab equipment not widely available. In view of the worldwide threat posed by the spread of new Influenza A variants, there is a need in the clinical arts for new and improved anti-viral medicinal agents. This invention meets these needs.
In one aspect, the invention provides methods for identifying whether a patient is infected with influenza virus type A, by determining whether NS1 protein of influenza virus type A is present in a patient sample, presence indicating the patient is infected with influenza virus type A. The determining step can involve contacting a patient sample with an agent that specifically binds to influenza virus type A protein NS1; and detecting specific binding between the agent and the NS1 protein, specific binding indicating presence of the influenza virus type A. Alternatively or in addition, the determining can include determining the presence of mRNA encoding the PDZ ligand motif (PL) of the NS1 protein and inferring presence of the NS1 protein from the presence of the mRNA. Preferably the PL has the motif: S/T-X-V/I/L where the S is serine, T is threonine, V is valine, I is isoleucine, L is leucine and X is any amino acid. Preferably, the agent is at least one PDZ polypeptide. Alternatively, the agent can be at least one antibody. For pan-specific antibodies, the antibody can be specific to a conserved region of the NS1 protein. Preferably, the contacting step involves contacting the patient sample with first and second agents that specifically bind to different epitopes of influenza virus type A protein NS1, and the first agent is immobilized on a support, and the detecting step detects a sandwich in which the first and second agents are specifically bound to the NS1 protein to indicate presence of the virus. The first and second agents can be first and second antibodies, but preferably, the first agent is one or more PDZ polypeptides and the second agent is one or more antibodies. The first agent can be a mixture of one or more PDZ polypeptides and one or more antibodies. The antibody can be an antibody specific for all subtypes of Influenza virus type A NS1.
The one or more PDZ polypeptides can be one or more of the following: Outer Membrane, PSD95 (PDZ #2), PSD95 (PDZ #1,2,3), DLG1 (PDZ #1), DLG1 (PDZ #1,2), DLG1 (PDZ #2), DLG2 (PDZ #1), DLG2 (PDZ #2), Magi3 (PDZ #1), PTN3 (PDZ #1), MAST2 (PDZ #1), NeDLG (PDZ #1,2), Shank1 d1, Shank2 d1, Shank3 d1, Syntrophin1 alpha, Syntrophin gamma 1, Magi1 (PDZ #1), Magi1 (PDZ #4), Tip1; PTPL1 (PDZ #1), Mint3 (PDZ #1), Lym Mystique (PDZ #1), DLG2 (PDZ #3), MUPP1 (PDZ #8), NeDLG (PDZ #1), DLG5 (PDZ #1), PSD95 (PDZ #1), NumBP (PDZ #3), LIMK1 (PDZ #1), KIAA0313, DLG1 (PDZ #2), Syntenin (PDZ #2), Pick1, MAST2, PTN3 (PDZ #1), NOS1 (PDZ #1, 2, 3), MINT1 (PDZ #2), ZO-1 (PDZ #2), NSP and RIM212.
The patient sample can be any of the following: blood, tissue, a nasal secretion, a lung exudate, a cloacal sample, a fecal sample, a throat swab and saliva. Preferably, the patient is a human, a bird, a swine, a horse, or a mammal. The PDZ polypeptide preferably includes the PL binding region (80-100 amino acid region), for example the PL binding region for PSD95 d2 is provided in SEQ ID NO:1. For subtype specific assays, the PDZ polypeptide is preferably PSD95 d1, PSD95 d2, PSD95 d3, INADL8d1, Magi1 d1, DLG1d2, DLG1d3, NeDLG1d1, or NeDLG1d2
In a further aspect, the invention provides methods for the diagnosis and typing of Influenza type A infections, by identifying the presence of subtype specific Influenza type A virus protein NS1 PDZ ligand motif (PL) regions. Preferably, the PL regions have the motif: S/T-X-V/I/L where the S is serine, T is threonine, V is valine, I is isoleucine, L is leucine and X is any amino acid.
In one aspect, the invention provides methods for detecting the presence and amount of Influenza virus type A protein containing a PL region in a test sample, by admixing an aliquot of a test sample with at least one PDZ peptide and at least one PDZ ligand (PL) detect reagent under conditions suitable for binding; and measuring the binding between the PDZ peptide and the PL detect reagent, a decrease in binding indicates the presence of Influenza virus type A protein in the test sample. Preferably, the Influenza virus type A protein is NP, HA, M1 or NS1. Preferably, the PL detect reagent includes the PL motif from the C-terminus of an Influenza virus type A protein selected from the group consisting of: NP, HA, M1 and NS1. Preferably the PL motif is: S/T-X-V/I/L where the S is serine, T is threonine, V is valine, I is isoleucine, L is leucine and X is any amino acid.
In a further aspect, the invention provides methods for identifying whether a patient is infected with influenza virus type A, by determining whether NS1 protein of influenza virus type A is present in a nasal secretion, a sputum sample or a throat swab from the patient, presence indicating the patient is infected with influenza virus type A.
In one aspect, the invention provides methods for detecting the presence and amount of Influenza virus type A protein containing a PL region in a test sample, by admixing an aliquot of a test sample with at least one PDZ peptide; and measuring the binding between the PDZ peptide and the PL Influenza virus type A protein, binding indicates the presence of Influenza virus type A protein in the test sample.
In a further aspect, the invention provides methods of determining whether a patient is infected with a pathogenic strain of influenza A, by determining whether a patient is infected with influenza A, and if the patient is infected, determining presence of a nonstructural protein with a PL motif in a patient sample, presence indicating that the patient is infected with a pathogenic strain of influenza virus type A.
In one aspect, the invention provides methods for identifying the presence of a specific subtype of an Influenza type A virus in a patient sample, by contacting a patient sample with at least one PDZ polypeptide or at least one capture antibody that specifically binds to a PL motif of an NS1 protein specific to a subtype of an influenza virus A; and detecting whether the PDZ polypeptide or capture antibody specifically binds to the PL motif in the sample, specific binding indicating presence of the subtype. Preferably, the contacting step involves contacting the patient sample with a plurality of PDZ polypeptides that specifically bind to a plurality of PL motifs in a plurality of NS1 proteins specific to a plurality of subtypes of influenza virus A; and the detecting involves determining which of the PDZ polypeptides specifically binds to its PL motif, the binding at one or more PDZ polypeptides thereby indicating presence of the subtype. Preferably, the capture antibody recognizes the carboxy terminus of NS1. Preferably, the capture antibody or PDZ polypeptide recognizes one or more of PDZ ligand motifs (PLs): ESEV (SEQ ID NO:2), ESEI (SEQ ID NO:3), ESKV (SEQ ID NO:4), TSEV (SEQ ID NO:5), GSEV (SEQ ID NO:6), RSEV (SEQ ID NO:7), RSKV (SEQ ID NO:8), GSEI (SEQ ID NO:9), GSKV (SEQ ID NO:10), NICI (SEQ ID NO:11), TICI (SEQ ID NO:12), RICI (SEQ ID NO:13), DMAL (SEQ ID NO:14), DMTL (SEQ ID NO:15), DIAL (SEQ ID NO:16), DLDY (SEQ ID NO:17), SICL (SEQ ID NO:18), SEV, SEI, SKV, and SKI. Preferably, the PDZ polypeptide is at least one of the following: Outer Membrane, PSD95 (PDZ #2), PSD95 (PDZ #1,2,3), DLG1 (PDZ #1), DLG1 (PDZ #1,2), DLG1 (PDZ #2), DLG2 (PDZ #1), DLG2 (PDZ #2), Magi3 (PDZ #1), PTN3 (PDZ #1), MAST2 (PDZ #1), NeDLG (PDZ #1,2), Shank1 d1, Shank2 d1, Shank3 d1, Syntrophin1 alpha, Syntrophin gamma 1, Magi1 (PDZ #1), Magi1 (PDZ #4), Tip1; PTPL1 (PDZ #1), Mint3 (PDZ #1), Lym Mystique (PDZ #1), DLG2 (PDZ #3), MUPP1 (PDZ #8), NeDLG (PDZ #1), DLG5 (PDZ #1), PSD95 (PDZ #1), NumBP (PDZ #3), LIMK1 (PDZ #1), KIAA0313, DLG1 (PDZ #2), Syntenin (PDZ #2), Pick1, MAST2, PTN3 (PDZ #1), NOS1 (PDZ #1, 2, 3), MINT1 (PDZ #2), ZO-1 (PDZ #2), NSP and RIM2. The patient sample can be a nasal secretion, a sputum sample, a throat swab, a cloacal sample, a fecal sample, a lung exudates, or saliva. If the method is used to identify a subtype, the subtype is preferably avian influenza A and the PL is the PL motif ESEV/I/A (SEQ ID NO:19). Alternatively the subtype is H3N2 and the PL is the PL motif RSKV (SEQ ID NO:8). Alternatively, the PL is the PL motif ESKV (SEQ ID NO:4). Alternatively, the subtype is H1N1 and the PL is the PL motif RSEV (SEQ ID NO:7). The method can also include contacting the sample with a detection antibody. Preferably, the detection antibody includes a signal generating compound and does not inhibit the binding of PL to the PDZ or the capture antibody to the NS1.
The PDZ polypeptide or antibody can be immobilized on a solid support. If the solid support is a capillary flow assay device the contacting step involves dipping the stick in the patient sample. Preferably, the capillary flow assay is an immunoassay. Preferably, the solid support is a lateral flow assay.
In one aspect, the invention provides kits for the identification and subtyping of Influenza A virus in a patient sample, having an agent that specifically binds to the Influenza A virus NS1 immobilized on a solid support. Preferably, the agent is an antibody, a PDZ polypeptide, an oligonucleotide aptamer, or a mixture.
In a further aspect, the invention provides kits for the identification and or subtyping of influenza A virus in a patient sample, including an agent that specifically binds to a Influenza A virally encoded protein; and an agent that specifically binds to an NS1 protein. Preferably, the agent that specifically binds to an NS1 protein, binds to the PL region on the protein. Preferably, the agent is an antibody, a PDZ polypeptide, an oligonucleotide aptamer, or a mixture. Preferably, the Influenza A virally encoded protein is NS1.
In one aspect, the invention provides kits for the identification and or subtyping of influenza A virus in a patient sample, including an agent that specifically binds to NS1 other than at a PL motif and an agent that specifically binds to NS1 at a PL motif.
In one aspect, the invention provides kits having a plurality of PDZ polypeptides specific for a plurality of PL motifs in a plurality of NS1 proteins of a plurality of influenza A viruses.
In one aspect, the invention provides methods for identifying a PDZ polypeptide capable of specifically binding to an influenza virus PDZ ligand (PL), by bringing the influenza virus non-structural protein PL into contact with a candidate polypeptide having a PDZ domain under conditions suitable for binding; detecting specific binding of the PL to the candidate polypeptide; and confirming that the PL is binding to the PDZ binding site.
In one aspect, the invention provides isolated antibodies that specifically bind to a carboxy-terminal motif in an NS1 protein of influenza virus type A. Preferably, the carboxy-terminal motif having a PL motif is ESEV (SEQ ID NO:2), ESEI (SEQ ID NO:3), ESKV (SEQ ID NO:4), TSEV (SEQ ID NO:5), GSEV (SEQ ID NO:6), RSEV (SEQ ID NO:7), RSKV (SEQ ID NO:8), GSEI (SEQ ID NO:9), GSKV (SEQ ID NO:10), NICI (SEQ ID NO:11), TICI (SEQ ID NO:12), RICI (SEQ ID NO:13), DMAL (SEQ ID NO:14), DMTL (SEQ ID NO:15), DIAL (SEQ ID NO:16), DLDY (SEQ ID NO:17), SICL (SEQ ID NO:18), SEV, SEI, SKV, or SKI. Preferably, the antibody is a monoclonal antibody or an antibody fragment. Preferably, the PL motif is ESEV/I/A (SEQ ID NO:19).
In one aspect, the invention provides methods for the treatment or prophylaxis of a patient having or at risk of an Influenza virus type A infection, by administering to the patient an effective regime of an agent that that inhibits interaction of an NS1 protein of the virus with a PDZ protein of the cell and thereby effecting treatment or prophylaxis of the infection. Preferably, the agent is an antibody that specifically binds to the PL motif of an NS1 protein of Influenza virus type A. Preferably, the agent is an antisense oligonucleotide, a small molecule, an siRNA or a zinc finger protein, and the agent inhibits expression of either the influenza A NS1 protein or a PDZ protein. Preferably, the PL motif of the NS1 is ESEV (SEQ ID NO:2), ESEI (SEQ ID NO:3), ESKV (SEQ ID NO:4), TSEV (SEQ ID NO:5), GSEV (SEQ ID NO:6), RSEV (SEQ ID NO:7), RSKV (SEQ ID NO:8), GSEI (SEQ ID NO:9), GSKV (SEQ ID NO:10), NICI (SEQ ID NO:11), TICI (SEQ ID NO:12), RICI (SEQ ID NO:13), DMAL (SEQ ID NO:14), DMTL (SEQ ID NO:15), DIAL (SEQ ID NO:16), DLDY (SEQ ID NO:17), SICL (SEQ ID NO:18), SEV, SEI, SKV, or SKI. Preferably, the agent is a PDZ polypeptide and it includes at least the binding region that interacts with a PL, SEQ ID NO:1. Preferably, the PDZ polypeptide is at least one of: Outer membrane, PSD95 (PDZ #2), PSD95 (PDZ #1,2,3), DLG1 (PDZ #1), DLG1 (PDZ #1,2), DLG1 (PDZ #2), DLG2 (PDZ #1), DLG2 (PDZ #2), Magi3 (PDZ #1), PTN3 (PDZ #1), MAST2 (PDZ #1), NeDLG (PDZ #1,2), Shank1 d1, Shank2 d1, Shank3 d1, Syntrophin1 alpha, Syntrophin gamma 1, Magi1 (PDZ #1), Magi1 (PDZ #4), Tip1; PTPL1 (PDZ #1), Mint3 (PDZ #1), Lym Mystique (PDZ #1), DLG2 (PDZ #3), MUPP1 (PDZ #8), NeDLG (PDZ #1), DLG5 (PDZ #1), PSD95 (PDZ #1), NumBP (PDZ #3), LIMK1 (PDZ #1), KIAA0313, DLG1 (PDZ #2), Syntenin (PDZ #2), Pick1, MAST2, PTN3 (PDZ #1), NOS1 (PDZ #1, 2, 3), MINT1 (PDZ #2), ZO-1 (PDZ #2), NSP and RIM2.
In a further aspect, the invention provides methods for screening for anti-viral agents, by contacting a PDZ polypeptide and an influenza viral PDZ ligand (PL) in the presence and absence of a test compound; and comparing the amount of PDZ/PL binding in the presence of the test compound as compared to the absence, preferably the anti-viral agent reduces PDZ/PL binding and may also include testing the agent in vivo or intracellularly to identify whether it interferes with Interferon production.
In one aspect, the invention provides non-natural PDZ ligand (PL) peptide diagnostic reagents, having a linear array of amino acids selected from within the C-terminal amino acid sequence of an Influenza A protein, such that the PL is capable of binding to a mammalian PDZ polypeptide. Preferably, the PL has the motif: S/T-X-V/I/L where the S is serine, T is threonine, V is valine, I is isoleucine, L is leucine and X is any amino acid. Preferably, the array of Influenza A NS1 proteins includes at least one of ESEV (SEQ ID NO:2), ESEI (SEQ ID NO:3), ESKV (SEQ ID NO:4), TSEV (SEQ ID NO:5), GSEV (SEQ ID NO:6), RSEV (SEQ ID NO:7), RSKV (SEQ ID NO:8), GSEI (SEQ ID NO:9), GSKV (SEQ ID NO:10), NICI (SEQ ID NO:11), TICI (SEQ ID NO:12), RICI (SEQ ID NO:13), DMAL (SEQ ID NO:14), DMTL (SEQ ID NO:15), DIAL (SEQ ID NO:16), DLDY (SEQ ID NO:17), SICL (SEQ ID NO:18), SEV, SEI, SKV or SKI. A diagnostic reagent such as a positive control, a negative control, an assay standard, an assay calibrator, a competition assay ligand, a labeled peptide detect agent or a solid-phase capture agent can also be included. A synthetic peptide, a recombinant polypeptide, a substantially purified natural PL polypeptide, a substantially purified fragment of a natural PL polypeptide, a peptide mimetic PL, an oligonucleotide aptamer PL or a polypeptide aptamer PL can also be included. Preferably, the PL peptide is from the Influenza A NS1 protein.
In one aspect, the invention provides a non-natural PDZ polypeptide diagnostic reagent for detecting an Influenza A PL in a biological sample having a non-natural PDZ polypeptide capable of binding to an Influenza A NS1 protein, preferably the PDZ domain protein diagnostic reagent is selected from the group of diagnostic reagents consisting of a positive control, a negative control, an assay standard, an assay calibrator, a competition ligand, a labeled protein detect binding partner and a capture agent, preferably Outer Membrane, PSD95 (PDZ #2), PSD95 (PDZ #1,2,3), DLG1 (PDZ #1), DLG1 (PDZ #1,2), DLG1 (PDZ #2), DLG2 (PDZ #1), DLG2 (PDZ #2), Magi3 (PDZ #1), PTN3 (PDZ #1), MAST2 (PDZ #1), NeDLG (PDZ #1,2), Shank1 d1, Shank2 d1, Shank3 d1, Syntrophin1 alpha, Syntrophin gamma 1, Magi1 (PDZ #1), Magi1 (PDZ #4), Tip1; PTPL1 (PDZ #1), Mint3 (PDZ #1), Lym Mystique (PDZ #1), DLG2 (PDZ #3), MUPP1 (PDZ #8), NeDLG (PDZ #1), DLG5 (PDZ #1), PSD95 (PDZ #1), NumBP (PDZ #3), LIMK1 (PDZ #1), KIAA0313, DLG1 (PDZ #2), Syntenin (PDZ #2), Pick1, MAST2, PTN3 (PDZ #1), NOS1 (PDZ #1, 2, 3), MINT1 (PDZ #2), ZO-1 (PDZ #2), NSP or RIM2.
In a further aspect, the invention provides signal generating conjugate agents for detecting an Influenza A protein in a test sample having a non-natural PL or a non-natural PDZ either of which PL or PDZ is a peptide or a polypeptide covalently linked with a signal generating compound.
In one aspect, the invention provides methods for identifying whether a patient is infected with a pathogenic influenza A, by determining whether NS2 protein of influenza virus type A is present in a patient sample, the protein having a Serine at position 70, presence indicating the patient is infected with a pathogenic strain of Influenza A. Preferably the determining step is contacting a patient sample with an agent that specifically binds to a sequence having the Serine 70. Preferably the agent is an antibody or a nucleic acid.
In one aspect, methods for identifying whether a patient is infected with a pathogenic avian influenza virus type A are provides that involve, contacting a patient sample with a PSD-95 PDZ protein; and detecting specific binding between the PSD-95 PDZ protein and the sample, specific binding indicating presence of the influenza virus type A, presence indicating the patient is infected with a pathogenic avian influenza virus type A. Preferably, the pathogenic influenza virus type A is H5N1. Preferably, the PSD-95 PDZ protein is domain 2 of PSD-95. Preferably, the influenza NS1 protein PL has a motif of ESKV (SEQ ID NO:4), ESEI (SEQ ID NO:3), or ESEV (SEQ ID NO:2). In one aspect, the contacting step involves contacting the patient sample with the PSD-95 PDZ protein and an antibody that specifically binds to a different epitope of influenza virus type A protein NS1 than the PSD-95 PDZ protein, and the PSD-95 is immobilized on a support, and the detecting step detects the NS1 protein specifically bound to the antibody. In a further aspect, the method includes another step of contacting the patient sample with a second PDZ protein, INADL d8 as a control and determining specific binding, a greater specific binding of the first PDZ-95 protein relative to the second PDZ protein, indicating that the patient is infected with a pathogenic avian influenza virus type A.
a-f are 11 exemplary lateral flow Influenza test formats.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by those of ordinary skill in the art to which this invention pertains. The following references provide one of skill with a general definition of many of the terms used in this invention: Singleton et al., DICTIONARY OF MICROBIOLOGY AND MOLECULAR BIOLOGY (2d ed. 1994); THE CAMBRIDGE DICTIONARY OF SCIENCE AND TECHNOLOGY (Walker ed., 1988); and Hale & Marham, THE HARPER COLLINS DICTIONARY OF BIOLOGY (1991). Although any methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present invention, the presently preferred methods and materials are described. Definitions are provided in a logical (rather than alphabetical) order to assist the reader in the practice of the invention, i.e., as follows: namely,
“Agent” includes any substance, molecule, element, compound, entity, or a combination thereof including but not limited to, e.g., proteins, polypeptides, small organic molecules, polysaccharide-peptide chimeric molecules, nucleotide-peptide chimeric molecules and the like. Representative examples of agents include natural products in a non-natural state, synthetic peptide compounds, chemical compounds, as well as, combinations of two or more natural or unnatural compounds. Unless otherwise specified, the terms “agent”, “substance”, and “compound” are used interchangeably.
“Avian influenza A” means an influenza A subtype that infects an avian subject and is transmissible between avian subjects. Representative examples of avian influenza hemagglutinin subtypes include H5, H6, H7, H9 and H10 and representative strains include H5N1, H6N2, H7N3, H7N7, H9N2, H10N4 and H10N5.
“Avian subject” means a subject suitable for testing or treatment including all species of birds, including both wild birds (such as wildfowl) and domesticated species (such as poultry). Preferably, the avian subject to be tested or treated is selected from the group consisting of chickens, turkeys, ducks, geese, quail, ostrich, emus and exotic birds such as parrots, cockatoos and cockatiels. More preferably, the avian subject to be tested is a chicken, turkey, goose or quail.
“Non-natural” is used to mean a composition not occurring in nature. Representative examples of non-natural compositions include substantially purified compositions, as well as, those containing compounds which do not appear in the same chemical form in nature, e.g., chemically and genetically modified proteins, nucleic acids and the like.
“Modulation” as used herein refers to both up-regulation, (i.e., activation or stimulation) for example by agonizing, and down-regulation (i.e. inhibition or suppression) for example by antagonizing a binding activity. As used herein, the term “PDZ ligand binding modulator” refers to an agent that is able to alter binding of a PDZ domain-containing polypeptide to a PDZ-ligand (i.e., “PL”). Modulators include, but are not limited to, both activators e.g. agonists and inhibitors e.g. antagonists. An inhibitor may cause partial or complete inhibition of binding.
“Pathogenic strain of influenza A” when used in the context of distinguishing between different strains of influenza virus means a “notifiable avian influenza” (NAI) virus according to the guidelines set forth by the OIE World Organization for Animal Health, World Health Organization or their designated representatives e.g., as set forth in the OIE “Manual of Diagnostic Tests and Vaccines for Terrestrial Animals, 5th edition, 2004 (www.oie.int). Further, the subject pathogenic strain has “high pathogenicity” in a representative test for virulence or an H5 or H7 virus with an influenza A hemagglutinin (HA) precursor protein HA0 cleavage site amino acid sequence that is similar to any of those that have been observed in virulent viruses, i.e., as defined by the OIE or a representative similar national or international organization or trade association. Representative examples of HA0 cleavage site amino acid sequences in virulent H5 and H7 strains of influenza A comprise multiple basic amino acids (arginine or lysine) at the cleavage site of the viral precursor hemagglutinin protein, e.g., where low virulence strains of H7 viruses have -PEIPKGR*GLF-(SEQ ID NO:20) or -PENPKGR*GLF-(SEQ ID NO:21) highly pathogenic strains have -PEIPKKKKR*GLF-(SEQ ID NO:22), -PETPKRKRKR*GLSF-(SEQ ID NO:23), -PEIPKKREKR*GLF-(SEQ ID NO:24) or -PETPKRRRR*GLF-(SEQ ID NO:25). Current representative tests for virulence include inoculation of 4-8 week old chickens with infectious virus wherein strains are considered to be highly pathogenic if they cause more than 75% mortality within 10 days; and/or, any virus that has an intravenous pathogenicity index (IVPI) greater than 1.2, wherein intravenously inoculated birds are examined at 24-hour intervals over a 10-day period; scored for “0”, normal; “1” sick; “2” severely sick”; “3” dead; and, the mean score calculated as the IVPI. The latter highly pathogenic strains are referred to by the OIE as a “highly pathogenic NAI virus” (HPNIA). Current representative examples of NAI include the H5 and H7 strains of influenza A. Current representative examples of HPNIA include H5N1.
“Less Pathogenic strain of influenza A” means an avian influenza A that is notifiable, i.e., an NAI isolate (supra), but which is not pathogenic for chickens and does not have an HA0 cleavage site amino acid sequence similar to any of those that have been observed in virulent viruses, i.e., a strain referred to by the OIE as a “low pathogenicity avian influenza (LPAI).
“PDZ domain” means an amino acid sequence homologous over about 90 contiguous amino acids; preferably about 80-90; more preferably, about 70-80, more preferably about 50-70 amino acids with the brain synaptic protein PSD-95, the Drosophila septate junction protein Discs-Large (DLG) and/or the epithelial tight junction protein ZO1 (ZO1). Representative examples of PDZ domains are also known in the art as Discs-Large homology repeats (“DHRs”) and “GLGF” repeats (SEQ ID NO:26). Examples of PDZ domains are found in diverse membrane-associated proteins including members of the MAGUK family of guanylate kinase homologs, several protein phosphatases and kinases, neuronal nitric oxide synthase, tumor suppressor proteins, and several dystrophin-associated proteins, collectively known as syntrophins. The instant PDZ domains encompass both natural and non-natural amino acid sequences. Representative examples of PDZ domains include polymorphic variants of PDZ proteins, as well as, chimeric PDZ domains containing portions of two different PDZ proteins and the like. Preferably, the instant PDZ domains contain amino acid sequences which are substantially identical to those disclosed in U.S. patent application Ser. No. 10/485,788 (filed Feb. 3, 2004), International patent application PCT/US03/285/28508 (filed Sep. 9, 2003), International patent application PCT/US01/44138 (filed Nov. 9, 2001), incorporated herein by reference in their entirety. Representative non-natural PDZ domains include those in which the corresponding genetic code for the amino acid sequence has been mutated, e.g., to produce amino acid changes that alter (strengthen or weaken) either binding or specificity of binding to PL. Optionally a PDZ domain or a variant thereof has at least 50, 60, 70, 80 or 90% sequence identity with a PDZ domain from at least one of brain synaptic protein PSD-95, the Drosophila septate junction protein Discs-Large (DLG) and/or the epithelial tight junction protein ZO1 (ZO1), and animal homologs. Optionally a variant of a natural PDZ domain has at least 90% sequence identity with the natural PDZ domain. Sequence identities of PDZ domains are determined over at least 70 amino acids within the PDZ domain, preferably 80 amino acids, and more preferably 80-90 or 80-100 amino acids. Amino acids of analogs are assigned the same numbers as corresponding amino acids in the natural human sequence when the analog and human sequence are maximally aligned. Analogs typically differ from naturally occurring peptides at one, two or a few positions, often by virtue of conservative substitutions. The term “allelic variant” is used to refer to variations between genes of different individuals in the same species and corresponding variations in proteins encoded by the genes. An exemplary PDZ domain for PSD-95 d2 is provided as SEQ ID NO:1.
“PDZ protein”, used interchangeably with “PDZ-domain containing polypeptides” and “PDZ polypeptides”, means a naturally occurring or non-naturally occurring protein having a PDZ domain (supra). Representative examples of PDZ proteins have been disclosed previously (supra) and include CASK, MPP1, DLG1, DLG2, PSD95, NeDLG, TIP-33, TIP-43, LDP, LIM, LIMK1, LIMK2, MPP2, AF6, GORASP1, INADL, KIAA0316, KIAA1284, MAGI1, MAST2, MINT1, NSP, NOS1, PAR3, PAR3L, PAR6 beta, PICK1, Shank 1, Shank 2, Shank 3, SITAC-18, TIP1, and ZO-1. The instant non-natural PDZ domain polypeptides useful in screening assays may contain e.g. a PDZ domain that is smaller than a natural PDZ domain. For example a non-natural PDZ domain may optionally contain a “GLGF” motif (SEQ ID NO:26), i.e., a motif having the GLGF amino acid sequence (SEQ ID NO:26), which typically resides proximal, e.g. usually within about 10-20 amino acids N-terminal, to an PDZ domain. The latter GLGF motif (SEQ ID NO:26), and the 3 amino acids immediately N-terminal to the GLGF motif (SEQ ID NO:26) are often required for PDZ binding activity. Similarly, non-natural PDZ domains may be constructed that lack the β-sheet at the C-terminus of a PDZ domain, i.e., this region may often be deleted from the natural PDZ domain without affecting the binding of a PL. Some exemplary PDZ proteins are provided and the GI or accession numbers are provided in parenthesis: PSMD9 (9184389), af6 (430993), AIPC (12751451), ALP (2773059), APXL-1 (13651263), MAGI2 (2947231), CARDI1 (1282772), CARDI4 (13129123), CASK (3087815), CNK1 (3930780), CBP (3192908), Densin 180 (16755892), DLG1 (475816), DLG2 (12736552), DLG5 (3650451), DLG6 splice var 1 (14647140), DLG6 splice var 2 (AB053303), DVL1 (2291005), DVL2 (2291007), DVL3 (6806886), ELFIN 1 (2957144), ENIGMA (561636), ERBIN (8923908), EZRIN binding protein 50 (3220018), FLJ00011 (10440342), FLJ11215 (11436365), FLJ12428 (BC012040), FLJ12615 (10434209), FLJ20075 Semcap2 (7019938), FLJ21687 (10437836), F1131349 (AK055911), F1132798 (AK057360), GoRASP1 (NM031899), GoRASP2 (13994253), GRIP1 (4539083), GTPase Activating Enzyme (2389008), Guanine Exchange Factor (6650765), HEMBA 1000505 (10436367), HEMBA 1003117 (7022001), HSPC227 (7106843), HTRA3 (AY040094), HTRA4 (AL576444), INADL (2370148), KIAA0147 Vartul (1469875), KIAA0303 MAST4 (2224546), KIAA0313 (7657260), KIAA0316 (6683123), KIAA0340 (2224620), KIAA0380 (2224700), KIAA0382 (7662087), KIAA0440 (2662160), KIAA0545 (14762850), KIAA0559 (3043641), KIAA0561 MAST3 (3043645), KIAA0613 (3327039), KIAA0751 RIM2 (12734165), KIAA0807 MAST2 (3882334), KIAA0858 (4240204), KIAA0902 (4240292), KIAA0967 (4589577), KIAA0973 SEMCAP3 (5889526), KIAA1202 (6330421), KIAA1222 (6330610), KIAA1284 (6331369), KIAA1389 (7243158), KIAA1415 (7243210), KIAA1526 (5817166), KIAA1620 (10047316), KIAA1634 MAGI3 (10047344), KIAA1719 (1267982), LIM Mystique (12734250), LIM (3108092), LIMK1 (4587498), LIMK2 (1805593), LIM-RIL (1085021), LU-1 (U52111), MAGI1 (3370997), MGC5395 (BC012477), MINT1 (2625024), MINT3 (3169808) MPP1 (189785), MPP2 (939884), MPP3 (1022812), MUPP1 (2104784), NeDLG (10853920), Neurabin II (AJ401189), NOS1 (642525), novel PDZ gene (7228177), Novel Serine Protease (1621243), Numb Binding Protein (AK056823), Outer Membrane Protein (7023825), p55T (12733367), PAR3 (8037914), PAR3-like (AF428250), PAR6 (2613011), PAR6BETA (13537116), PAR6GAMMA (13537118), PDZ-73 (5031978), PDZK1 (2944188), PICK1 (4678411), PIST (98394330), prIL16 (1478492), PSAP (6409315), PSD95 (3318652), PTN-3 (179912), PTN-4 (190747), PTPL1 (515030), RGS12 (3290015), RGS3 (18644735), Rho-GAP10 (NMO20824), Rhophilin-like (14279408), Serine Protease (2738914), Shank 2 (6049185), Shank 3 (AC000036), Shroom (18652858), Similar to GRASP65 (14286261), Similar to Ligand of Numb px2 (BC036755), Similar to PTP Homolog (21595065), SIP1 (2047327), SITAC-18 (8886071), SNPCIIA (20809633), Shank 1 (7025450), Syntenin (2795862), Syntrophin 1 alpha (1145727), Syntrophin beta 2 (476700), Syntrophin gamma 1 (9507162), Syntrophin gamma 2 (9507164), TAX2-like protein (3253116), TIAM 1 (4507500), TIAM 2 (6912703), TIP 1 (2613001), TIP2 (2613003), TIP33 (2613007), TIP43 (2613011), X-11 beta (3005559), ZO-1 (292937), ZO-2 (12734763), ZO-3 (10092690).
“PDZ ligand”, abbreviated “PL”, means a naturally occurring protein that has an amino acid sequence which binds to and forms a molecular interaction complex with a PDZ-domain. Representative examples of PL have been provided previously in prior US and International patent applications (supra). Additional examples of influenza A PL are provided in the Examples section, below.
“PDZ agent” is used to mean a compound that interferes with the binding interaction occurring between a PDZ ligand polypeptide and a PDZ domain-containing polypeptide in a test assay by at least 20%, e.g., at least 30%, at least 40%, at least 50%, at least 60%, at least 70%, at least 80%, at least 90%, at least 95%, up to about 99% or 100%, as compared to controls that do not include the PDZ agent. While not wishing to be limited to any particular mechanism of action, the instant PDZ agent may interfere e.g. by binding to a PDZ domain that would otherwise bind to an influenza NS1 ligand; or alternatively, it may bind directly to the NS1 ligand to prevent its binding to the PDZ protein. In general, the latter PDZ agents are those which exhibit IC50s in a particular assay in the range of about 1 mM or lower. Compounds which exhibit lower IC50s, for example, commonly have IC50s of about 100 μM, 10 μM, 1 μM, 100 nM, 10 nM, 1 nM, or even lower. The latter PDZ agents are useful in therapeutic and prophylactic medicinal compositions administered to alleviate, treat or prevent one or more symptoms of disease resulting from infection with an influenza A virus. “PL modulator” is used in the context of a PDZ agent (supra) to mean a compound that binds to an influenza A NS1 protein and modulates its binding to a PDZ domain.
“PDZ modulator” is used in the context of a PDZ agent (supra) to mean a compound that binds to a PDZ domain and modulates the binding of an influenza NS1 protein at the subject PDZ domain site.
The instant PDZ modulators and PL modulators may be peptides, peptidomimetics or small molecule mimetics designed to bind a PDZ domain or PL, respectively. Assays for determining whether a PDZ modulator binds to a PDZ domain are described in great detail in the Examples section, below. Similarly, assays for determining whether a PL modulator binds to a PDZ domain are set forth, e.g., recombinant PDZ domain fusion proteins binding to recombinant NS1 fusion proteins.
“PDZ-mediated disorder” means one or more symptoms in an Influenza A infected subject that result from binding of an influenza A viral protein PL at a host cell PDZ domain. The latter symptoms caused by viral infection, include, but are not limited to fever, cough, sore throat, muscle aches, conjunctivitis, breathing problems, excessive mucus production in the airways, increased susceptibility to secondary bacterial infection, pneumonia, neural infection and the like.
“Sick” when used herein to refer to an avian subject, includes signs and symptoms which may vary from sudden death with few overt signs of disease to a more characteristic disease with respiratory signs, excessive lacrimation, sinusitis, edema of the head, cyanosis of the unfeathered skin and diarrhea. Representative diagnostic signs, specimens and tests of “sick” disclosed by OIE in their health guidelines “Manual of Diagnostic Tests and Vaccines for Terrestrial Animals, 5th edition, 2004, World Organization for Animal Health” are incorporated herein by reference in their entirety.
“Analog” is used herein to refer to a molecule that structurally resembles a natural PDZ or PL molecule of interest but which has been modified e.g. by replacing or chemically modifying one or more selected amino acid substituents. Compared to the starting molecule, an analog may exhibit the same, similar or improved utility. Synthesis and screening of analogs to identify variants of known compounds having improved traits is well known in the medicinal arts, e.g., increasing binding affinity, altering selectivity of binding to a target, lowering binding to non-target molecules, improving stability in vitro and in vivo and improving pharmacologic properties.
“Contacting” has its normal meaning and refers to combining two or more agents so that constituents are thereby brought together, e.g., a PL in a test sample is brought together with a PDZ. Contacting can occur in vitro, e.g., a PDZ protein is brought together with a cell lysate in a test tube or other container; or, in situ, e.g., a natural host cell PDZ protein and a natural viral PL are brought together in an influenza infected cell by virtue of the natural biosynthetic activities of the cell. Alternatively, a recombinant PDZ is brought together with a viral PL by e.g. transfecting a PDZ domain coding sequence into an influenza A infected cell.
“Polymer” is used to refer to a serial array of one or more types of repeating units, regardless of the source. Polymers may be found in biological systems and particularly include polypeptides and polynucleotides, as well as, compounds containing amino acids, nucleotides, or analogs thereof. The term “polynucleotide” refers to a polymer of nucleotides, or analogs thereof, of any length, including oligonucleotides that range from 10-100 nucleotides in length and polynucleotides of greater than 100 nucleotides in length. The term “polypeptide” refers to a polymer having a serial array of amino acids of any length, preferably in the range of about 12 to about 50 amino acids in serial array; and, most preferably greater than about 50 amino acids.
“Polypeptide” and “protein” are used interchangeably to include polymeric serial arrays of amino acids in which the natural peptide-bond backbone has been replaced with non-natural synthetic backbones, and polypeptides in which one or more of the natural amino acids have been replaced with one or more non-naturally occurring or synthetic mimetic amino acids.
“Fusion protein” means a polypeptide composed of amino acid sequences derived from two or more natural proteins which are expressed as a single recombinant protein, i.e., two or more amino acid sequences that while not attached in their native state are joined together in the recombinant protein e.g. by their respective amino and carboxyl termini through a peptide linkage to form a single continuous amino acid sequence. Fusion proteins may be a combination of two, three or even four or more different natural or non-natural proteins. Representative fusion proteins include those with two or more heterologous, i.e., unrelated, amino acid sequences; those with both heterologous and homologous, i.e., related, sequences. Fusion proteins also consist of amino acid sequences with or without N-terminal methionine residues, those tagged for identification with antigenic epitopes, as well as, those having a signal generating compound as a fusion partner, e.g., fusion proteins with a fluorescent partner; an enzyme partner such as β-galactosidase; a chemilluminescent partner such as luciferase; and the like.
“Capture agent”, when used in the context of a diagnostic assay reagent or method, refers to an agent that is capable of binding to an influenza viral analyte in a binding interaction that is of sufficient strength, e.g. measured as a binding affinity, and specificity that it enables concentration of the viral analyte from within a mixture of different viral analytes; and, in a time period suitable for use in an a diagnostic assay format, i.e., typically about 5 minutes to about 90 minutes; preferably about 5 minutes to about 60 minutes; and, most preferably about 5 minutes to about 30 minutes. According to alternative embodiments of the invention, the instant capture agents are contain either a PDZ domain or a PL. Representative capture agents are illustrated in the Examples section below. Capture agents usually “specifically bind” one or more viral analytes, e.g., PL containing proteins, to the exclusion of other analytes, e.g., proteins that do not contain a PL. Preferably, the instant capture agents bind the subject viral analyte with a dissociation constant (KD) that is less than about 10−6 M; preferably, less than about 10−7M; and, most preferably, less than about 10−8 M.
“Specific binding”, when used in regard to the binding interaction between the instant natural and non-natural PDZ domain and PL reagents, is used to refer to the ability of a capture- or detect-agent to preferentially bind to a particular viral analyte that is present in a mixture of different viral analytes. In certain embodiments, the subject specific binding interaction is capable of discriminating between proteins having or lacking a PL, i.e., in some embodiments the discriminatory capacity is greater than about 10- to about 100-fold; and, preferably greater than about 1000- to about 10,000-fold.
The term “substantial identity” means that two peptide sequences, when optimally aligned, such as by the programs GAP or BESTFIT using default gap weights, share at least 65 percent sequence identity, preferably at least 80 or 90 percent sequence identity, more preferably at least 95 percent sequence identity or more (e.g., 99 percent sequence identity or higher). Preferably, residue positions which are not identical differ by conservative amino acid substitutions.
“Binding interference”, is used in regard to the first binding interaction of a PDZ domain with a PL to form a complex in a diagnostic assay format; wherein, the subject complex is subsequently detected in a requisite second binding interaction, i.e., interference results when the first binding interaction inhibits the second binding interaction resulting in a decrease in the strength of the signal produced by a signal generating compound. The signal generated by the instant compositions in the methods of the invention are subject to less than 15% binding interference; preferably, less than 10%; and, most preferably less than about 5%.
“Capture agent/analyte complex” is a complex that results from the specific binding of a capture agent, e.g. a PDZ domain fusion protein, with an analyte, e.g. an influenza viral protein having a PL. A capture agent and an analyte specifically bind, i.e., the one to the other, under “conditions suitable for specific binding”, wherein such physicochemical conditions are conveniently expressed e.g. in terms of salt concentration, pH, detergent concentration, protein concentration, temperature and time. The subject conditions are suitable to allow binding to occur e.g. in a solution; or alternatively, where one of the binding members is immobilized on a solid phase. Representative conditions so-suitable are well known in the diagnostic arts e.g. see, Harlow and Lane, “Antibodies: A Laboratory Manual”, Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y. (1989). Suitable conditions preferably result in binding interactions having dissociation constants (KD) that are less than about 10−6M; preferably, less than about 10−7M; and, most preferably less than about 10−8M.
“Surface-bound capture agent” is used interchangeably with “solid-phase capture agent” to refer to a PDZ domain or PL capture agent that is immobilized on a surface of a solid substrate, e.g., a sheet, bead, or other structure, such as a plate with wells and the like as set forth in greater detail below. In certain embodiments, the collections of capture agents employed herein are present on a surface of the same support, e.g., in the form of an array wherein a particular location on a surface is correspond to the presence of a particular surface-bound capture agent.
“Isolated” or “purified” generally refers to isolation of a substance (compound, polynucleotide, protein, polypeptide, polypeptide composition) such that the substance comprises a significant percent (e.g., greater than 2%, greater than 5%, greater than 10%, greater than 20%, greater than 50%, or more, usually up to about 90%-100%) of the sample in which it resides. In certain embodiments, a substantially purified component comprises at least 50%, 80%-85%, or 90-95% of the sample. Techniques for purifying polynucleotides and polypeptides of interest are well-known in the art and include, for example, ion-exchange chromatography, affinity chromatography and sedimentation according to density. Generally, a substance is purified when it exists in a sample in an amount, relative to other components of the sample, that is not found naturally.
“Assessing”, when used in the context of the instant assay, refers to evaluating a test result and/or conducting a test measurement to determine whether an influenza A viral analyte is present in a test sample. Representative evaluations include “determining”, “measuring”, “evaluating”, “assessing” and “assaying”, as they may be used interchangeably to include quantitative and/or qualitative determinations. Assessing may be relative or absolute. “Assessing binding” includes determining the amount or extent of a binding interaction, as well as, determining whether particular binding interaction has occurred, i.e., whether binding is present or absent.
“Treatment”, “treating”, “treat”, and the like, refer to administering a compound according the invention to a subject in need thereof with the aim of achieving a desired pharmacologic and/or physiologic effect, e.g., preventing or alleviating one or more symptoms of disease (supra). The treatment may be administered in a prophylactic manner, i.e., to prevent development of one or more symptoms of disease; and/or, therapeutically, to reduce or eliminate a disease symptom. Subjects in need thereof include mankind and domesticated animals.
“Subject”, is used herein to refer to a man and domesticated animals, e.g. mammals, fishes, birds, reptiles, amphibians and the like.
“Signal generating compound”, abbreviated “SGC”, means a molecule that can be linked to a PL or a PDZ (e.g. using a chemical linking method as disclosed further below and is capable of reacting to form a chemical or physical entity (i.e., a reaction product) detectable in an assay according to the instant disclosure. Representative examples of reaction products include precipitates, fluorescent signals, compounds having a color, and the like. Representative SGC include e.g., bioluminescent compounds (e.g., luciferase), fluorophores (e.g., below), bioluminescent and chemiluminescent compounds, radioisotopes (e.g., 131I, 125I, 14C, 3H, 35S, 32P and the like), enzymes (e.g., below), binding proteins (e.g., biotin, avidin, streptavidin and the like), magnetic particles, chemically reactive compounds (e.g., colored stains), labeled-oligonucleotides; molecular probes (e.g., CY3, Research Organics, Inc.), and the like. Representative fluorophores include fluorescein isothiocyanate, succinyl fluorescein, rhodamine B, lissamine, 9,10-diphenlyanthracene, perylene, rubrene, pyrene and fluorescent derivatives thereof such as isocyanate, isothiocyanate, acid chloride or sulfonyl chloride, umbelliferone, rare earth chelates of lanthanides such as Europium (Eu) and the like. Representative SGC's useful in a signal generating conjugate include the enzymes in: IUB Class 1, especially 1.1.1 and 1.6 (e.g., alcohol dehydrogenase, glycerol dehydrogenase, lactate dehydrogenase, malate dehydrogenase, glucose-6-phosphate dehydrogenase, glyceraldehyde-3-phosphate dehydrogenase and the like); IUB Class 1.11.1 (e.g., catalase, peroxidase, amino acid oxidase, galactose oxidase, glucose oxidase, ascorbate oxidase, diaphorase, urease and the like); IUB Class 2, especially 2.7 and 2.7.1 (e.g., hexokinase and the like); IUB Class 3, especially 3.2.1 and 3.1.3 (e.g., alpha amylase, cellulase, β-galacturonidase, amyloglucosidase, β-glucuronidase, alkaline phosphatase, acid phosphatase and the like); IUB Class 4 (e.g., lyases); IUB Class 5 especially 5.3 and 5.4 (e.g., phosphoglucose isomerase, trios phosphatase isomerase, phosphoglucose mutase and the like.) Signal generating compounds also include SGC whose products are detectable by fluorescent and chemilluminescent wavelengths, e.g., luciferase, fluorescence emitting metals such as 152Eu, or others of the lanthanide series; compounds such as luminol, isoluminol, acridinium salts, and the like; bioluminescent compounds such as luciferin; fluorescent proteins; and the like. Fluorescent proteins include, but are not limited to the following: namely, (i) green fluorescent protein (GFP), i.e., including, but not limited to, a “humanized” versions of GFP wherein codons of the naturally-occurring nucleotide sequence are exchanged to more closely match human codon bias; (ii) GFP derived from Aequoria victoria and derivatives thereof, e.g., a “humanized” derivative such as Enhanced GFP, which are available commercially, e.g., from Clontech, Inc.; (iii) GFP from other species such as Renilla reniformis, Renilla mulleri, or Ptilosarcus guernyi, as described in, e.g., WO 99/49019 and Peelle et al. (2001) J. Protein Chem. 20:507-519; (iv) “humanized” recombinant GFP (hrGFP) (Stratagene); and, (v) other fluorescent and colored proteins from Anthozoan species, such as those described in Matz et al. (1999) Nature Biotechnol. 17:969-973; and the like. The subject signal generating compounds may be coupled to a PL or PDZ domain polypeptide. Attaching certain SGC to proteins can be accomplished through metal chelating groups such as EDTA. The subject SGC share the common property of allowing detection and/or quantification of an influenza PL analyte in a test sample. The subject SGC are detectable using a visual method; preferably, an a method amenable to automation such as a spectrophotometric method, a fluorescence method, a chemilluminescent method, a electrical nanometric method involving e.g., a change in conductance, impedance, resistance and the like and a magnetic field method.
“Solid phase”, as used herein, means a surface to which one or more reactants may be attached electrostatically, hydrophobically, or covalently. Representative solid phases include e.g.: nylon 6; nylon 66; polystyrene; latex beads; magnetic beads; glass beads; polyethylene; polypropylene; polybutylene; butadiene-styrene copolymers; silastic rubber; polyesters; polyamides; cellulose and derivatives; acrylates; methacrylates; polyvinyl; vinyl chloride; polyvinyl chloride; polyvinyl fluoride; copolymers of polystyrene; silica gel; silica wafers glass; agarose; dextrans; liposomes; insoluble protein metals; and, nitrocellulose. Representative solid phases include those formed as beads, tubes, strips, disks, filter papers, plates and the like. Filters may serve to capture analyte e.g. as a filtrate, or act by entrapment, or act by covalently-binding PL or PDZ onto the filter (e.g., see the Examples section below). According to certain embodiments of the invention, a solid phase capture reagent for distribution to a user may consist of a solid phase (supra) coated with a “capture reagent” (below), and packaged (e.g., under a nitrogen atmosphere) to preserve and/or maximize binding of the capture reagent to an influenza PL analyte in a biological sample.
“Capture reagent” means an immobilized PDZ polypeptide (or peptide) capable of binding an influenza PL. The subject capture reagent may consist of a solution of a PDZ; or a PDZ modified so as to promote its binding to a solid phase; or a PDZ already immobilized onto the surface of a solid phase, e.g., immobilized by attaching the PDZ to a solid phase (supra) through electrostatic forces, van Der Waals forces, hydrophobic forces, covalent chemical bonds, and the like (as disclosed further below.) Representative examples of PDZ capture reagents are disclosed in the Examples section, below, and include mobile solid phase PDZ capture reagents such as PDZ immobilized on movable latex beads e.g. in a latex bead dipstick assay.
“Detect reagent” means a conjugate containing an SGC linked to a PL or P) DZ polypeptide or peptide; or alternatively, an SGC linked to an antibody capable of binding specifically to a PL or a PDZ. Representative examples of the instant detect reagents include complexes of one or more PL or PDZ with one or more SGC compounds, i.e., macromolecular complexes. The subject detect reagents include mobile solid-phase detect reagents such as movable latex beads in latex bead dipstick assays.
“Biological sample” means a sample obtained from a living (or dead) organism, e.g., a mammal, fish, bird, reptile, marsupial and the like. Biological samples include tissue fluids, tissue sections, biological materials carried in the air or in water and collected there from e.g. by filtration, centrifugation and the like, e.g., for assessing bioterror threats and the like. Alternative biological samples can be taken from fetus or egg, egg yolk, and amniotic fluids. Representative biological fluids include, e.g. urine, blood, plasma, serum, cerebrospinal fluid, semen, lung lavage fluid, feces, sputum, mucus, water carrying biological materials and the like. Alternatively, biological samples include nasopharyngeal or oropharyngeal swabs, nasal lavage fluid, tissue from trachea, lungs, air sacs, intestine, spleen, kidney, brain, liver and heart, sputum, mucus, water carrying biological materials, cloacal swabs, sputum, nasal and oral mucus, and the like. Representative biological samples also include foodstuffs, e.g., samples of meats, processed foods, poultry, swine and the like. Biological samples also include contaminated solutions (e.g., food processing solutions and the like), swab samples from out-patient sites, hospitals, clinics, food preparation facilities (e.g., restaurants, slaughter-houses, cold storage facilities, supermarket packaging and the like). Biological samples may also include in-situ tissues and bodily fluids (i.e., samples not collected for testing), e.g., the instant methods may be useful in detecting the presence or severity or viral infection in the eye e.g., using eye drops, test strips applied directly to the conjunctiva; or, the presence or extent of lung infection by e.g. placing an indicator capsule in the mouth or nasopharynx of the test subject. Alternatively, a swab or test strip can be placed in the mouth. The biological sample may be derived from any tissue, organ or group of cells of the subject. In some embodiments a scrape, biopsy, or lavage is obtained from a subject. Biological samples may include bodily fluids such as blood, urine, sputum, and oral fluid; and samples such as nasal washes, swabs or aspirates, tracheal aspirates, chancre swabs, and stool samples. Methods are known to those of skill in the art for the collection of biological specimens suitable for the detection of individual pathogens of interest, for example, nasopharyngeal specimens such as nasal swabs, washes or aspirates, or tracheal aspirates in the case of high risk influenza A viruses involved in respiratory disease, oral swabs and the like. Thus, embodiments of the invention provide methods useful in testing a variety of different types of biological samples for the presence or amount of a influenza A contamination or infection. Optionally, the biological sample may be suspended in an isotonic solution containing antibiotics such as penicillin, streptomycin, gentamycin, and mycostatin.
“Ligand” as used herein refers to a PL compound capable of binding to an PDZ binding site. Representative examples of ligands include PL-containing complex viral particles (supra) as found in a variety of different strains of influenza A. The subject ligand is capable of filling a three-dimensional space in binding site of a PDZ domain binding site so that electrostatic repulsive forces are minimized, electrostatic attractive forces are maximized, and hydrophobic and hydrogen bonding forces are maximized. Ligands bind to PDZ polypeptides in a specific and saturable manner, and binding affinities may be measured according to ligand binding assays known to those skilled in the art, e.g. as disclosed further below.
“Specificity”, when used in the context of an assay according to an embodiment of the invention, means that the subject assay, as performed according to the steps of the invention, is capable of properly identifying an “indicated” percentage of samples from within a panel of biological samples (e.g., a panel of 100 samples). The subject panel of samples all contain one or more murein analytes (e.g., positive control samples contaminated with bacteria or fungi.) Preferably the subject “indicated” specificity is greater than 85%, (e.g., the assay is capable of indicating that more than 85 of the 100 samples contain one or more murein analyte), and most preferably, the subject assay has an indicated specificity that is greater than 90%. Optionally, the subject assay is capable of identifying “true non-influenza A cases”, i.e., detecting an “indicated” percentage of negative samples from within a panel of biological samples (e.g., a panel of 100 samples). Preferably, the instant steps of the invention are capable of properly identifying “true non-avian influenza A cases”; and most preferably, the instant steps of the invention are capable of properly identifying “true low-pathogenic avian influenza A cases”. In different embodiments, the subject negative control panel of samples either do not contain influenza A PL analytes; or, contain non-avian influenza A PL analytes; or, contain non-pathogenic influenza A PL. Preferably the subject specificity is greater than 85%, (e.g., the assay is capable of indicating that more than 85 of the 100 samples and most preferably, the subject assay has specificity that is greater than 90%.
“Sensitivity”, when used in the context of an assay according to an embodiment of the invention, means that the subject assay, as performed according to the steps of the invention, is capable of identifying at an “indicated” percentage those samples which contain an influenza PL analyte from within a panel of samples containing both positive controls (supra) and negative controls (i.e., lacking PL analyte.) Preferably the subject “indicated” sensitivity is greater than 85% and most preferably greater than 90%. Optionally, the subject assay is capable of identifying “true influenza A cases” at an “indicated” percentage of those samples which contain an influenza PL analyte from within a panel of samples. Preferably, the instant steps of the invention are capable of properly identifying “true avian influenza A cases”; and, most preferably, the instant steps of the invention are capable of properly identifying “true pathogenic avian influenza A cases”. In different embodiments, the subject positive control panel of samples either contain influenza A PL analytes; or, contain avian influenza A PL analytes; or, contain highly pathogenic influenza A PL. Preferably the subject “indicated” sensitivity is greater than about 70% and more preferably greater than about 80%. Even more preferably, the sensitivity is greater than about 85% and most preferably greater than about 90% of that of the control. Alternatively, the sensitivity can be measured with respect to the sensitivity of a PCR reaction that identifies the same protein
With respect to Specificity and Sensitivity, optionally, the following definitions can be applied:
“Positive predictive value”, abbreviated PPV, means the percentage of samples that test positive in the instant method and are true avian influenza A cases. Preferably, the instant method has a PPV greater than about 65% and most preferably greater than about 80%.
“Negative predictive value”, abbreviated NPV, means the percentage of samples the percentage of samples that test negative and are true negative influenza A cases. Preferably, the instant method has an NPV greater than about 85% and most preferably greater than about 90%.
“True positive influenza A” when used in reference to a biological sample means a sample containing influenza A virion particles as confirmed in two or more independent tests, e.g., isolation and cultivation in embryonated chicken eggs, identification of viral antigen in a commercial immunoassay test, immunodiffusion, hemagglutination and/or hemagglutination inhibition testing to identify the HA and/or NA subtype, RT-PCR detection of viral RNA or immunofluorescence detection of influenza A antigen in cells in respiratory specimens.
“True positive avian influenza A” when used in reference to a biological sample means a sample containing avian influenza A virion particles as confirmed in two or more independent tests, e.g., isolation and cultivation in embryonated chicken eggs, identification of viral antigen in a commercial immunoassay test, immunodiffusion, hemagglutination and/or hemagglutination inhibition testing to identify the HA and/or NA subtype, RT-PCR detection of viral RNA or immunofluorescence detection of influenza A antigen in cells in respiratory specimens.
“True positive highly pathogenic avian influenza A” when used in reference to a biological sample means a sample containing highly pathogenic avian influenza A virion particles as defined supra and as confirmed in two or more independent tests, e.g., isolation and cultivation in embryonated chicken eggs, identification of viral antigen in a commercial immunoassay test, immunodiffusion, hemagglutination and/or hemagglutination inhibition testing to identify the HA and/or NA subtype RT-PCR detection of viral RNA or immunofluorescence detection of influenza A antigen in cells in respiratory specimens.
“True negative influenza A” when used in reference to a biological sample means a sample that does not contain influenza A virion particles as confirmed in two or more independent tests, e.g., isolation and cultivation in embryonated chicken eggs, identification of viral antigen in a commercial immunoassay test, immunodiffusion, hemagglutination and/or hemagglutination inhibition testing to identify the HA and/or NA subtype RT-PCR detection of viral RNA or immunofluorescence detection of influenza A antigen in cells in respiratory specimens.
“True negative avian influenza A” when used in reference to a biological sample means a sample that does not contain avian influenza A virion particles as confirmed in two or more independent tests, e.g., isolation and cultivation in embryonated chicken eggs, identification of viral antigen in a commercial immunoassay test, immunodiffusion, hemagglutination and/or hemagglutination inhibition testing to identify the HA and/or NA subtype, RT-PCR detection of viral RNA or immunofluorescence detection of influenza A antigen in cells in respiratory specimens. In this case, the biological sample may contain influenza A virion particles other than avian influenza A virion particles, i.e., as defined supra.
“True negative highly pathogenic avian influenza A” when used in reference to a biological sample means a sample does not contain highly pathogenic avian influenza A virion particles as defined supra and as confirmed in two or more independent tests, e.g., isolation and cultivation in embryonated chicken eggs, identification of viral antigen in a commercial immunoassay test, immunodiffusion, hemagglutination and/or hemagglutination inhibition testing to identify the HA and/or NA subtype, RT-PCR detection of viral RNA or immunofluorescence detection of influenza A antigen in cells in respiratory specimens. The subject sample may however contain influenza A virion particles or lower pathogenicity avian influenza A virion particles as defined supra.
“Background”, when used in the context of an assay according to an embodiment of the invention, means the uncertainty in a test result, (sometime expressed as a percentage of false-positive or false-negative test results or by a measurement of a degree of confidence in a test result), occasioned by substances which may interfere with the proper performance of the assay when they are present in the assay. Representative examples of substances which may so interfere, i.e., interfering substances, confounding substances, and the like, include endogenous PDZ binding polypeptides, inhibitors or substrates for signal generating compounds, e.g., enzyme inhibitors, free radical reactive compounds, endogenous peroxides and the like.
“Substantially purified” is used herein to refer to a preparation that contains a natural PDZ or PL polypeptide or peptide in a non-natural state e.g. a higher level of purity than in nature. Representative higher levels of purity than recorded in natural samples include PDZ and PL polypeptides and fragments thereof that are enriched greater than about 10-fold to about 25-fold, preferably greater than about 26-fold to about 50-fold and most preferably greater than about 100-fold from the levels present in a natural source material. The subject preparation also preferably contains less than about 10% impurities, and most preferably less than about 5% impurities detectable e.g. by either SDS-PAGE or reverse-phase HPLC.
Nucleic acid and protein sequences that have been previously determined and electronically deposited into NCBI's Genbank database are referenced herein by Genbank accession number (GI). The sequences set forth in those Genbank entries are incorporated by reference herein in their entirety for all purposes. The Applicants expressly reserve the right to later amend the specification to specifically recite one or more of these sequences, or any indicated portion thereof.
Various biochemical and molecular biology methods referred to herein are well known in the art, and are described in, for example, Sambrook et al., Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Press, N.Y. Second (1989) and Third (2000) Editions, and Current Protocols in Molecular Biology, (Ausubel, F. M. et al., eds.) John Wiley & Sons, Inc., New York (1987-1999).
A nucleic acid can be DNA or RNA, and single- or double-stranded. Oligonucleotides can be naturally occurring or synthetic, but are typically prepared by synthetic means. Preferred nucleic acids of the invention include segments of DNA, or their complements including any one of the NS2 sequences comprising Ser 70 shown in Table 12. The segments are usually between 5 and 100 contiguous bases, and often range from 5, 10, 12, 15, 20, or 25 nucleotides to 10, 15, 30, 25, 20, 50 or 100 nucleotides. Nucleic acids between 5-10, 5-20, 10-20, 12-30, 15-30, 10-50, 20-50 or 20-100 bases are common. The polymorphic site can occur within any position of the segment. The segments can be from any of the allelic forms of NS2 shown in Table 12. For brevity in the table, the symbol T is used to represent both thymidine in DNA and uracil in RNA. Thus, in RNA oligonucleotides, the symbol T should be construed to indicate a uracil residue.
Hybridization probes are capable of binding in a base-specific manner to a complementary strand of nucleic acid. Such probes include nucleic acids, peptide nucleic acids, as described in Nielsen et al., Science 254, 1497-1500 (1991).
The term primer refers to a single-stranded oligonucleotide capable of acting as a point of initiation of template-directed DNA synthesis under appropriate conditions (i.e., in the presence of four different nucleoside triphosphates and an agent for polymerization, such as, DNA or RNA polymerase or reverse transcriptase) in an appropriate buffer and at a suitable temperature. The appropriate length of a primer depends on the intended use of the primer but typically ranges from 15 to 40 nucleotides. Short primer molecules generally require cooler temperatures to form sufficiently stable hybrid complexes with the template. A primer need not reflect the exact sequence of the template but must be sufficiently complementary to hybridize with a template. The term primer site refers to the area of the target DNA to which a primer hybridizes. The term primer pair means a set of primers including a 5′ upstream primer that hybridizes with the 5′ end of the DNA sequence to be amplified and a 3′, downstream primer that hybridizes with the complement of the 3′ end of the sequence to be amplified.
Polymorphism refers to the occurrence of two or more genetically determined alternative sequences or alleles in a population of viruses. The first identified allelic form is arbitrarily designated as the reference form and other allelic forms are designated as alternative or variant alleles. In this case, the polymorphism comprises the position 70 in which Glycine is replaced with Serine.
A single nucleotide polymorphism occurs at a polymorphic site occupied by a single nucleotide, which is the site of variation between allelic sequences. The site is usually preceded by and followed by highly conserved sequences of the allele (e.g., sequences that vary in less than 1/100 or 1/1000 members of the populations).
A single nucleotide polymorphism usually arises due to substitution of one nucleotide for another at the polymorphic site. A transition is the replacement of one purine by another purine or one pyrimidine by another pyrimidine. A transversion is the replacement of a purine by a pyrimidine or vice versa. Single nucleotide polymorphisms can also arise from a deletion of a nucleotide or an insertion of a nucleotide relative to a reference allele.
A set of polymorphisms means at least 2, and sometimes 5, or more of the polymorphisms shown in Tables 12 or 13 and/or Tables 3a-e.
Hybridizations are usually performed under stringent conditions that allow for specific binding between an oligonucleotide and a target DNA containing one of the polymorphic sites shown in Tables 12 or 13 and/or Tables 3a-e. A stringent condition is defined as any suitable buffer concentrations and temperatures that allow specific hybridization of the oligonucleotide to highly homologous sequence spanning at least one of the polymorphic sites shown in Table 12 or 13 and any washing conditions that remove non-specific binding of the oligonucleotide. For example, conditions of 5×SSPE (750 mM NaCl, 50 mM Na Phosphate, 5 mM EDTA, pH 7.4) and a temperature of 25-30° C. are suitable for allele-specific probe hybridizations.
The washing conditions usually range from room temperature to 60° C.
The term “primer” refers to a single-stranded oligonucleotide capable of acting as a point of initiation of template-directed DNA synthesis under appropriate conditions (i.e., in the presence of four different nucleoside triphosphates and an agent for polymerization, such as, DNA or RNA polymerase or reverse transcriptase) in an appropriate buffer and at a suitable temperature. The appropriate length of a primer depends on the intended use of the primer but typically ranges from 15 to 30 nucleotides, although shorter or longer primers can also be used. Short primer molecules generally require cooler temperatures to form sufficiently stable hybrid complexes with the template. A primer need not reflect the exact sequence of the template but must be sufficiently complementary to hybridize with a template. The term “primer site” refers to the area of the target DNA to which a primer hybridizes. The term “primer pair” means a set of primers including a 5′ upstream primer that hybridizes with the 5′ end of the DNA sequence to be amplified and a 3′, downstream primer that hybridizes with the complement of the 3′ end of the sequence to be amplified.
For purposes of classifying amino acids substitutions as conservative or nonconservative, amino acids are grouped as follows: Group I (hydrophobic sidechains): norleucine, met, ala, val, leu, ile; Group II (neutral hydrophilic side chains): cys, ser, thr; Group III (acidic side chains): asp, glu; Group IV (basic side chains): asn, gln, his, lys, arg; Group V (residues influencing chain orientation): gly, pro; and Group VI (aromatic side chains): trp, tyr, phe. Conservative substitutions involve substitutions between amino acids in the same class. Non-conservative substitutions constitute exchanging a member of one of these classes for a member of another.
Methods recited herein may be carried out in any order of the recited events, i.e., to the extent that such order is logically possible. Furthermore, where a range of values is provided, it is understood that every intervening value, between the upper and lower limit of the recited range, as well as, any other stated or intervening value falling within the subject range is encompassed within the invention. Also, it is contemplated that any optional feature of the inventive variations described may be set forth and claimed independently, or in combination with any one or more of the features described herein.
Reference to a singular item includes the possibility that there are plural of the same items present. More specifically, as used herein and in the appended claims, the singular forms “a,” “an,” “said” and “the” include plural referents unless the context clearly dictates otherwise. It is further noted that the claims may be drafted to exclude any optional element. As such, this statement is intended to serve as antecedent basis for use of such exclusive terminology as “solely,” “only” and the like in connection with the recitation of claim elements, or use of a “negative” limitation.
The invention is premised in part on the insight that the influenza NS1 proteins possess a PL region that interacts with mammalian PDZ proteins and that different PL motifs interact specifically with different PDZ proteins. The invention is further premised in part on the result that detectable levels of the NS1 PL protein can be found in body secretions, such as nasal secretions. Influenza A usurps normal host cell functions and triggers changes that result in pathogenicity. It has been discovered that certain pathogenic strains of influenza have nonstructural NS1 proteins with ligand motifs that bind to mammalian PDZ proteins. As emergent virulence factors, NS1 proteins likely interfere with, or divert, PDZ proteins assembly of host cell macromolecular protein complexes. Since PDZ proteins are also normally involved in chaperone, endocytic and secretory processes, evidence disclosed herein is highly supportive of the notion that virulent influenza strains disrupt cellular PDZ-based regulatory mechanisms. The invention provides novel diagnostic compositions and methods, as well as, therapeutic anti-viral targets and candidate compounds.
The present results show that specific PDZ proteins bind influenza NS1 with high affinity and specificity. PDZ proteins bind C-terminal tri- and tetra-peptide NS1 motifs in virulent, but not in non-virulent, strains of influenza A. As an illustration of the methods, utilizing recombinant PDZ proteins and cross-reactive anti-NS1 monoclonal antibodies, chimeric assays were constructed to distinguish between pathogenic and non-pathogenic strains of influenza A (also called virulent and non-virulent). The assay methods involved contacting a test sample from a subject with a PDZ-domain containing polypeptide and detecting whether a pathogenic influenza A NS1 PDZ ligand in the sample bound to the PDZ ligand polypeptide. Binding between the PDZ-containing polypeptide and the viral PDZ ligand indicated that the NS1 was from a virulent strain of influenza A. This result when using the assay for a patient sample indicates that the subject is infected with a pathogenic strain of influenza A virus. The assay is particularly suitable to identify the pathogenic strains H5 and/or H7. More preferably the assay identifies at least one pathogenic strain including H5N1, H7N2, H7N7, H10N7, and most preferably the assay identifies the strain H5N1. More preferably, the assay identifies a pathogenic strain that is an avian strain, such as that currently causal for avian influenza, H5N1 having the NS1 PL motif ESEV (SEQ ID NO:2).
The various strains of influenza A encode proteins that have different PDZ ligands (PL). The various strains of influenza A can, therefore, be distinguished on the basis of their PL. Thus, the invention also provides methods for determining the sub-type of an influenza virus by the correlation with a specific NS1 PL class. Methods are also provided for determining whether a human subject is infected with an avian H5N1 strain of influenza virus. Assays for identifying anti-viral agents are also provided. Because the instant methods detect viral NS1 antigens that are produced only inside infected cells, the instant methods are useful in screening to detect subjects that are currently infected. The method is particularly advantageous because, unlike other methods, it can distinguish between vaccinated and infected subjects. Infected subjects have viral NS1 antigens, whereas vaccinated do not. Most preferably, the instant method is capable of distinguishing between the different subtypes of avian influenza A virus to identify, i.e., with a positive test result, one or more highly pathogenic strains of avian influenza A if they are present in a biological sample. Preferably, the instant test methods comprise steps for monitoring avian subjects for infection with a highly pathogenic strain of avian influenza A such as H5N1 or H7, e.g., in a commercial slaughter house facility, farm or breeding facility. In other embodiments, the invention provides methods for preventing the spread of an influenza A virus epidemic in a plurality of subjects by identifying infected animals and removing and/or destroying and/or treating them to prevent transmission to other subjects. Preferably, the instant methods comprise distinguishing avian and human subjects that are infected with a highly pathogenic influenza A strain, e.g., an avian subtype such as H5N1, from those who are infected with a lower pathogenicity strain.
The invention additionally provides a method for determining if a subject is infected with an influenza virus; and/or, whether the subject is infected with a high risk avian strain of influenza A virus. The method involves contacting a test sample from the subject with a PDZ-domain polypeptide, antibody, and/or aptamer and/or other agent, that specifically recognizes an NS1 PL, and determining whether a binding interaction occurs between an analyte in the test sample and the PDZ domain polypeptide, antibody, and/or aptamer. Assessing and detecting the subject binding interaction serves to determine that the test sample contains an influenza virus PL; thereby identifying that the subject is infected. The instant methods can also distinguish between the strains of influenza A virus, e.g., assessing whether a subject is infected with a high risk strain (pathogenic) of avian influenza virus such as H5N1, or alternatively, with a lower risk H1N1 strain (not pathogenic). Screening assays useful for identifying medicinal anti-viral compounds, e.g. in pharmaceutical development, are also provided. Thus, the invention finds uses in a variety of diagnostic and therapeutic applications.
The influenza viruses belong to the Orthomyxoviridae family, and are classified into groups A, B, and C based upon antigenic differences in their nucleoprotein (NP) and matrix protein (M1). Further subtyping into strains is commonly based upon assessing the type of antigen present in two virion glycoproteins, namely, hemagglutinin (HA; H) and neuraminidase (NA; N). HA and NP are virulence factors mediating attachment of the virion to the surface of host cells. M1 protein is thought to function in virus assembly and budding, while NP functions in RNA replication and transcription. In addition to these virion proteins, two other non-structural, i.e., non-virion, proteins are expressed in virus infected cells which are referred to as non-structural proteins 1 and 2 (NS1; NS2). The non-structural viral protein NS1 has multiple functions including the regulation of splicing and nuclear export of cellular mRNAs and stimulation of translation, as well as the counteracting of host interferon ability. The NS1 protein has been identified and sequenced in influenza viruses and the sequence can be found in the NCBI database. The NS1 protein in other influenza viruses, means a protein having the greatest sequence similarity to one of the proteins identified as NS1 proteins in known influenza subtypes, using as sequence for example, genbank accession numbers, CY003340, CY003324, DQ266101, etc.
All avian influenza viruses are classified as type A. Type A viruses have been isolated from humans, pigs, horses and sea mammals as well as both domestic and wild birds. Avian influenza viruses are key contributors to the emergence of human influenza pandemics, as both the Asian flu of 1957 and the Hong Kong flu of 1968 were caused by viruses believed to have been derived from avian sources. In recent years pure avian influenza viruses, of subtypes H5N1 and H7N7, have directly caused fatal human illnesses in Hong Kong and in Holland (Horimoto, T. and Kawaoka, Y. (2001) Clin. Microbiol. Rev. 14: 129-149; Guan, Y. et al. (2004) Proc. Natl. Acad. Sci. USA 101: 8156-8161).
The examples below show that the Influenza viral pathogens contain viral proteins having motifs for PDZ ligands that bind to PDZ proteins. The viral proteins having PL motifs, include the hemagglutinin (HA), nucleoprotein (NP), matrix 1 (M1) and non-structural protein 1 (NS1) proteins. However, the class II PL motifs (in all but the NS1 proteins) show a weaker binding for PDZ proteins. The PL motifs can typically be found in the last three or four C-terminal amino acids of the protein. An identifiable motif found in the majority of influenza NS1 proteins is S/T-X-V/I/L, where the S is serine, T is threonine, V is valine, I is isoleucine, L is leucine and X is any amino acid. The frequency of each specific motif is shown in Example 1, and Tables 3a-e). Although EPEV (SEQ ID NO:27) and KMAD (SEQ ID NO:28) do not correspond to typical PL motifs, they bind to PDZs at some level and can also be used for identification. The results in Table 3a-e and
PDZ domains have recently emerged as central organizers of protein complexes at the plasma membrane. PDZ domains were originally identified as conserved sequence elements within the postsynaptic density protein PSD95/SAP90, the Drosophila tumor suppressor dlg-A, and the tight junction protein ZO-1. Although originally referred to as GLGF (SEQ ID NO:26) or DHR motifs, they are now known by an acronym representing these first three PDZ-containing proteins (PDZ: PSD95/DLG/ZO-1). These 80-90 amino acids sequences have now been identified in well over 75 proteins and are characteristically expressed in multiple copies within a single protein. PDZ domains are recognized as families by the National Center for Biotechnology Information (www.ncbi.gov) for example in Pfam. They are also found throughout phylogeny in organisms as diverse as metazoans, plants, and bacteria. Such a broad species distribution appears to be unique to this domain, but perhaps the most distinguishing feature of PDZ domains is the observation that the overwhelming majority of proteins containing them are associated with the plasma membrane. Although PDZ domains are found in many different structures, each PDZ protein is generally restricted to specific subcellular domains, such as synapses; cell-cell contacts; or the apical, basal, or lateral cell surface. This leads to the speculation that PDZ domains evolved early to provide a central role in the organization of plasma membrane domains. The most general function of PDZ domains may be to localize their ligands to the appropriate plasma membrane domain. In polarized epithelial cells, PDZ proteins clearly localize at distinct apical, basal-lateral, and junctional membrane domains and, in most cases, colocalize with their transmembrane and cytosolic binding partners. PDZ proteins also clearly have a fundamental role spatially clustering and anchoring transmembrane proteins within specific subcellular domains.
PDZ domains contain ˜80-90 residues that fold into a structure with a beta-sandwich of 5-6 beta-strands and two alpha-helices. The peptide ligand binds in a hydrophobic cleft composed of a beta-strand (bB), an alpha-helix and a loop that binds the peptide carboxylate group. The peptide binds in an anti-parallel fashion to the bB strand, with the C-terminal residue occupying a hydrophobic pocket. PDZ heterodimers form a linear head-to-tail arrangement that involves recognition of an internal on one of the partner proteins. PDZ domain proteins are known in the art and new proteins can be identified as having PDZ domains by sequencing the protein and identifying the presence of a PDZ domain. PDZ proteins are explained in detail and a large number of examples are given in U.S. patent application Ser. No. 10/485,788, filed Aug. 2, 2004. Alternatively, a protein suspected of being a PDZ protein can be tested for binding to a variety of PL proteins or NS1 PL classes.
NS1 proteins from influenza containing the PL motif bound to PDZ proteins as shown in the Examples. Methods used to identify binding are shown in Example 2. Two complementary assays (the A and G assays) to detect binding between a PDZ-domain polypeptide and candidate PDZ ligand polypeptide are set out in detail in U.S. patent application Ser. Nos. 10/485,788, filed Aug. 2, 2004 and 10/714,537, filed Nov. 14, 2003. In each of the two different assays, binding is detected between a peptide having a sequence corresponding to the C-terminus of a protein anticipated to bind to one or more PDZ domains (i.e. a candidate PL peptide) and a PDZ-domain polypeptide (typically a fusion protein containing a PDZ domain).
A. Assays for Detection of Interactions Between PDZ-Domain Polypeptides and NMDA Receptor PL Proteins
Two complementary assays, termed “A” and “G,” were developed to detect binding between a PDZ-domain polypeptide and candidate PDZ ligand. In each of the two different assays, binding is detected between a peptide having a sequence corresponding to the C-terminus of a protein anticipated to bind to one or more PDZ domains (i.e. a candidate PL peptide) and a PDZ-domain polypeptide (typically a fusion protein containing a PDZ domain). In the “A” assay, the candidate PL peptide is immobilized and binding of a soluble PDZ-domain polypeptide to the immobilized peptide is detected (the “A” assay is named for the fact that in one embodiment an avidin surface is used to immobilize the peptide). In the “G” assay, the PDZ-domain polypeptide is immobilized and binding of a soluble PL peptide is detected (The “G” assay is so-named because a GST-binding surface is used to immobilize the PDZ-domain polypeptide). Exemplary assays are described below.
I. “A Assay” Detection of PDZ-Ligand Binding Using Immobilized PL Peptide.
The assay involves the following:
1) Biotinylated candidate PL peptides are immobilized on an avidin coated surface. The binding of PDZ-domain fusion protein to this surface is then measured.
(2) Avidin is bound to a surface, e.g. a protein binding surface. Optionally, avidin is bound to a polystyrene 96 well plate (e.g., Nunc Polysorb (cat #475094) by addition of 100 μL per well of 20 μg/mL of avidin (Pierce) in phosphate buffered saline without calcium and magnesium, pH 7.4 (“PBS”, GibcoBRL) at 4° C. for 12 hours. The plate is then treated to block nonspecific interactions by addition of 200 μL per well of PBS containing 2 g per 100 mL protease-free bovine serum albumin (“PBS/BSA”) for 2 hours at 4° C. The plate is then washed 3 times with PBS by repeatedly adding 200 μL per well of PBS to each well of the, plate and then dumping the contents of the plate into a waste container and tapping the plate gently on a dry surface.
(3) Biotinylated PL peptides (or candidate PL peptides) are immobilized on the surface of wells of the plate by addition of 50 μL per well of 0.4 μM peptide in PBS/BSA for 30 minutes at 4° C. Usually, each different peptide is added to at least eight different wells so that multiple measurements (e.g. duplicates and also measurements using different (GST/PDZ-domain fusion proteins and a GST alone negative control) can be made, and also additional negative control wells are prepared in which no peptide is immobilized. Following immobilization of the PL peptide on the surface, the plate is washed 3 times with PBS.
(4) GST/PDZ-domain fusion protein is allowed to react with the surface by addition of 50 μL per well of a solution containing 5 μg/mL GST/PDZ-domain fusion protein in PBS/BSA for 2 hours at 4° C. As a negative control, GST alone (i.e. not a fusion protein) is added to specified wells, generally at least 2 wells (i.e. duplicate measurements) for each immobilized peptide. After the 2 hour reaction, the plate is washed 3 times with PBS to remove unbound fusion protein.
(5) The binding of the GST/PDZ-domain fusion protein to the avidin-biotinylated peptide surface can be detected using a variety of methods, and detectors known in the art. In one embodiment, 50 μL per well of an anti-GST antibody in PBS/BSA (e.g. 2.5 μg/mL of polyclonal goat-anti-GST antibody, Pierce) is added to the plate and allowed to react for 20 minutes at 4° C. The plate is washed 3 times with PBS and a second, detectably labeled antibody is added. In one embodiment, 50 μL per well of 2.5 μg/mL of horseradish peroxidase (HRP)-conjugated polyclonal rabbit anti-goat immunoglobulin antibody is added to the plate and allowed to react for 20 minutes at 4° C. The plate is washed 5 times with 50 mM Tris pH 8.0 containing 0.2% Tween 20, and developed by addition of 100 μL per well of HRP-substrate solution (TMB, Dako) for 20 minutes at room temperature (RT). The reaction of the HRP and its substrate is terminated by the addition of 100 μL per well of 1M sulfuric acid and the optical density (O.D.) of each well of the plate is read at 450 nm.
(6) Specific binding of a PL peptide and a PDZ-domain polypeptide is detected by comparing the signal from the well(s) in which the PL peptide and PDZ domain polypeptide are combined with the background signal(s). The background signal is the signal found in the negative controls. Typically a specific or selective reaction will be at least twice background signal, more typically more than 5 times background, and most typically 10 or more times the background signal. In addition, a statistically significant reaction involves multiple measurements of the reaction with the signal and the background differing by at least two standard errors, more typically four standard errors, and most typically six or more standard errors. Correspondingly, a statistical test (e.g. a T-test) comparing repeated measurements of the signal with repeated measurements of the background will result in a p-value<0.05, more typically a p-value<0.01, and most typically a p-value<0.001 or less. As noted, in an embodiment of the “A” assay, the signal from binding of a GST/PDZ-domain fusion protein to an avidin surface not exposed to (i.e. not covered with) the PL peptide is one suitable negative control (sometimes referred to as “B”). The signal from binding of GST polypeptide alone (i.e. not a fusion protein) to an avidin-coated surface that has been exposed to (i.e. covered with) the PL peptide is a second suitable negative control (sometimes referred to as “B2”). Because all measurements are done in multiples (i.e. at least duplicate) the arithmetic mean (or, equivalently, average) of several measurements is used in determining the binding, and the standard error of the mean is used in determining the probable error in the measurement of the binding. The standard error of the mean of N measurements equals the square root of the following: the sum of the squares of the difference between each measurement and the mean, divided by the product of (N) and (N−1). Thus, in one embodiment, specific binding of the PDZ protein to the plate-bound PL peptide is determined by comparing the mean signal (“mean S”) and standard error of the signal (“SE”) for a particular PL-PDZ combination with the mean B1 and/or mean B2.
II. “G Assay”—Detection of PDZ-Ligand Binding Using Immobilized PDZ-Domain Fusion Polypeptide
In one aspect, the invention provides an assay in which a GST/PDZ fusion protein is immobilized on a surface (“G” assay). The binding of labeled PL peptide (for example one of those listed in
(1) A PDZ-domain polypeptide is bound to a surface, e.g. a protein binding surface. In a preferred embodiment, a GST/PDZ fusion protein containing one or more PDZ domains is bound to a polystyrene 96-well plate. The GST/PDZ fusion protein can be bound to the plate by any of a variety of standard methods, although some care must be taken that the process of binding the fusion protein to the plate does not alter the ligand-binding properties of the PDZ domain. In one embodiment, the GST/PDZ fusion protein is bound via an anti-GST antibody that is coated onto the 96-well plate. Adequate binding to the plate can be achieved when:
(2) Biotinylated PL peptides are allowed to react with the surface by addition of 50 μL per well of 20 μM solution of the biotinylated peptide in PBS/BSA for 10 minutes at 4° C., followed by an additional 20 minute incubation at 25° C. The plate is washed 3 times with ice cold PBS.
(3) The binding of the biotinylated peptide to the GST/PDZ fusion protein surface can be detected using a variety of methods and detectors known to one of skill in the art. In an exemplary procedure, 100 μL per well of 0.5 μg/mL streptavidin-horse radish peroxidase (HRP) conjugate dissolved in BSA/PBS is added and allowed to react for 20 minutes at 4° C. The plate is then washed 5 times with 50 mM Tris pH 8.0 containing 0.2% Tween 20, and developed by addition of 100 μL per well of HRP-substrate solution (TMB, Dako) for 20 minutes at room temperature (RT). The reaction of the HRP and its substrate is terminated by addition of 100 μL per well of 1 M sulfuric acid, and the optical density (O.D.) of each well of the plate is read at 450 um.
(4) Specific binding of a PL peptide and a PDZ domain polypeptide is determined by comparing the signal from the well(s) in which the PL peptide and PDZ domain polypeptide are combined, with the background signal(s). The background signal is the signal found in the negative control(s). Typically a specific or selective reaction is at least twice background signal, more typically more than 5 times background, and most typically 10 or more times the background signal. In addition, a statistically significant reaction involves multiple measurements of the reaction with the signal and the background differing by at least two standard errors, more typically four standard errors, and most typically six or more standard errors. Correspondingly, a statistical test (e.g. a T-test) comparing repeated measurements of the signal with -repeated measurements of the background will result in a p-value<0.05, more typically a p-value<0.01, and most typically a p-value<0.001 or less. As noted, in an embodiment of the “G” assay, the signal from binding of a given PL peptide to immobilized (surface bound) GST polypeptide alone is one suitable negative control (sometimes referred to as “B 1”). Because all measurement are done in multiples (i.e. at least duplicate) the arithmetic mean (or, equivalently, average) of several measurements is used in determining the binding, and the standard error of the mean is used in determining the probable error in the measurement of the binding. The standard error of the mean of N measurements equals the square root of the following: the sum of the squares of the difference between each measurement and the mean, divided by the product of (N) and (N−1). Thus, in one embodiment, specific binding of the PDZ protein to the platebound peptide is determined by comparing the mean signal (“mean S”) and standard error of the signal (“SE”) for a particular PL-PDZ combination with the mean B1.
i) “G′ assay” and “G″ assay”
Two specific modifications of the specific conditions described supra for the “G assay” are particularly useful. The modified assays use lesser quantities of labeled PL peptide and have slightly different biochemical requirements for detection of PDZ-ligand binding compared to the specific assay conditions described supra.
For convenience, the assay conditions described in this section are referred to as the “G′ assay” and the “G″ assay,” with the specific conditions described in the preceding section on G assays being referred to as the “G0 assay.” The “G' assay” is identical to the “G0 assay” except at step (2) the peptide concentration is 10 uM instead of 20 uM. This results in slightly lower sensitivity for detection of interactions with low affinity and/or rapid dissociation rate. Correspondingly, it slightly increases the certainty that detected interactions are of sufficient affinity and half-life to be of biological importance and useful therapeutic targets.
The “G″ assay” is identical to the “G0 assay” except that at step (2) the peptide concentration is 1 μM instead of 20 μM and the incubation is performed for 60 minutes at 25° C. (rather than, e.g., 10 minutes at 4° C. followed by 20 minutes at 25° C.). This results in lower sensitivity for interactions of low affinity, rapid dissociation rate, and/or affinity that is less at 25° C. than at 4° C. Interactions will have lower affinity at 25° C. than at 4° C. if (as we have found to be generally true for PDZ-ligand binding) the reaction entropy is negative (i.e. the entropy of the products is less than the entropy of the reactants). In contrast, the PDZ-PL binding signal may be similar in the “G″ assay” and the “G0 assay” for interactions of slow association and dissociation rate, as the PDZ-PL complex will accumulate during the longer incubation of the “G″ assay.” Thus comparison of results of the “G″ assay” and the “G0 assay” can be used to estimate the relative entropies, enthalpies, and kinetics of different PDZ-PL interactions. (Entropies and enthalpies are related to binding affinity by the equations delta G=RT ln (Kd)=delta H−T delta S where delta G, H, and S are the reaction free energy, enthalpy, and entropy respectively, T is the temperature in degrees Kelvin, R is the gas constant, and Kd is the equilibrium dissociation constant). In particular, interactions that are detected only or much more strongly in the “G0 assay” generally have a rapid dissociation rate at 25° C. (t1/2<10 minutes) and a negative reaction entropy, while interactions that are detected similarly strongly in the “G″ assay” generally have a slower dissociation rate at 25° C. (t1/2>10 minutes). Rough estimation of the thermodynamics and kinetics of PDZ-PL interactions (as can be achieved via comparison of results of the “G0 assay” versus the “G″ assay” as outlined supra) can be used in the design of efficient inhibitors of the interactions. For example, a small molecule inhibitor based on the chemical structure of a PL that dissociates slowly from a given PDZ domain (as evidenced by similar binding in the “G″ assay” as in the “G0 assay”) may itself dissociate slowly and thus be of high affinity.
In this manner, variation of the temperature and duration of step (2) of the “G assay” can be used to provide insight into the kinetics and thermodynamics of the PDZ-ligand binding reaction and into design of inhibitors of the reaction.
The detectable labels of the invention can be any detectable compound or composition which is conjugated directly or indirectly with a molecule (such as described above). The label can be detectable by itself (e.g., radioisotope labels or fluorescent labels) or, in the case of an enzymatic label, can catalyze a chemical alteration of a substrate compound or composition which is detectable. The preferred label is an enzymatic one which catalyzes a color change of a non-radioactive color reagent.
Sometimes, the label is indirectly conjugated with the antibody. One of skill is aware of various techniques for indirect conjugation. For example, the antibody can be conjugated with biotin and any of the categories of labels mentioned above can be conjugated with avidin, or vice versa (see also “A” and “G” assay above). Biotin binds selectively to avidin and thus, the label can be conjugated with the antibody in this indirect manner. See, Ausubel, supra, for a review of techniques involving biotin-avidin conjugation and similar assays. Alternatively, to achieve indirect conjugation of the label with the antibody, the antibody is conjugated with a small hapten (e.g. digoxin) and one of the different types of labels mentioned above is conjugated with an anti-hapten antibody (e.g. anti-digoxin antibody). Thus, indirect conjugation of the label with the antibody can be achieved.
Assay variations can include different washing steps. By “washing” is meant exposing the solid phase to an aqueous solution (usually a buffer or cell culture media) in such a way that unbound material (e.g., non-adhering cells, non-adhering capture agent, unbound ligand, receptor, receptor construct, cell lysate, or HRP antibody) is removed therefrom. To reduce background noise, it is convenient to include a detergent (e.g., Triton X) in the washing solution. Usually, the aqueous washing solution is decanted from the wells of the assay plate following washing. Conveniently, washing can be achieved using an automated washing device. Sometimes, several washing steps (e.g., between about 1 to 10 washing steps) can be required.
Various buffers can also be used in PDZ-PL detection assays. For example, various blocking buffers can be used to reduce assay background. The term “blocking buffer” refers to an aqueous, pH buffered solution containing at least one blocking compound which is able to bind to exposed surfaces of the substrate which are not coated with a PL or PDZ-containing protein. The blocking compound is normally a protein such as bovine serum albumin (BSA), gelatin, casein or milk powder and does not cross-react with any of the reagents in the assay. The block buffer is generally provided at a pH between about 7 to 7.5 and suitable buffering agents include phosphate and TRIS.
Various enzyme-substrate combinations can also be utilized in detecting PDZ-PL interactions. Examples of enzyme-substrate combinations include, for example:
(i) Horseradish peroxidase (HRPO) with hydrogen peroxidase as a substrate, wherein the hydrogen peroxidase oxidizes a dye precursor (e.g. orthophenylene diamine [OPD] or 3,3′,5,5′-tetramethyl benzidine hydrochloride [TMB]) (as described above).
(ii) alkaline phosphatase (AP) with para-Nitrophenyl phosphate as chromogenic substrate.
(iii) β-D-galactosidase (β D-Gal) with a chromogenic substrate (e.g. p-nitrophenyl-β-D-galactosidase) or fluorogenic substrate 4-methylumbelliferyl-β-D-galactosidase. Numerous other enzyme-substrate combinations are available. For a general review of these, see U.S. Pat. Nos. 4,275,149 and 4,318,980, both of which are herein incorporated by reference.
TABLES 1 and 2, on the following page, list PDZ domain-containing proteins (“PDZ proteins”) and PDZ ligands (“PL”) which have been identified as herein as binding to one another. Each of the PL proteins has binding affinity for at least one PDZ protein. The second column of TABLE 1 lists the influenza A protein from which the PL protein is derived (for example, hemagglutinin (HA), nucleoprotein (NP), matrix (M1) and non-structural protein 1 (NS1); the third column lists the PL motif amino acid sequence; and the fourth column provides the GenBank identification number (GI number) for the PDZ domain proteins binding to the PL (which database entries are incorporated by reference herein, including any annotation described therein).
PDZ proteins can be produced as fusion proteins, as long as they contain an active PDZ domain. For example, PDZ domains cloned into a vector (PGEX-3X vector) for production of GST-PDZ fusion proteins (Pharmacia) have been produced and taught in prior US and International patent applications, e.g., U.S. patent application Ser. No. 10/485,788 (filed Feb. 3, 2004), International patent application PCT/US03/285/28508 (filed Sep. 9, 2003), International patent application PCT/US01/44138 (filed Nov. 9, 2001), incorporated herein by reference in their entirety.
Methods of screening can include the use of sequence analysis to identify PDZ domains using any computer program known for the use of sequence analysis and/or domain analysis. Once a PDZ protein is identified, it can be screened for the ability to interact with influenza PL proteins.
A PDZ protein or PDZ domain polypeptide is any protein that contains a PDZ domain. Any protein containing a PDZ domain, whether natural, recombinant, chimeric or a fragment can be screened for its ability to bind to an influenza PL domain. Methods of identification of PDZ domains are given in U.S. patent application Ser. No. 10/485,788 (filed Feb. 3, 2004), International patent application PCT/US03/285/28508 (filed Sep. 9, 2003), International patent application PCT/US01/44138 (filed Nov. 9, 2001), incorporated herein by reference in their entirety.
PL binding agents suitable for use in a diagnostic assay include any agent that specifically binds to one or more PL motifs. Such agents can be identified using the same methods as disclosed in methods of screening for anti-viral agents. For example, agents can be identified using a protein containing a PL motif. Test compounds can be identified using any type of library, including expression libraries and small molecule libraries for example. A preferred source of test compounds for use in screening for therapeutics or therapeutic leads is a phage display library. See, e.g., Devlin, WO 91/18980; Key, B. K., et al., eds., Phage Display of Peptides and Proteins, A Laboratory Manual, Academic Press, San Diego, Calif., 1996. Phage display is a powerful technology that allows one to use phage genetics to select and amplify peptides or proteins of desired characteristics from libraries containing 108-109 different sequences. Libraries can be designed for selected variegation of an amino acid sequence at desired positions, allowing bias of the library toward desired characteristics. Libraries are designed so that peptides are expressed fused to proteins that are displayed on the surface of the bacteriophage. The phage displaying peptides of the desired characteristics are selected and can be regrown for expansion. Since the peptides are amplified by propagation of the phage, the DNA from the selected phage can be readily sequenced facilitating rapid analyses of the selected peptides.
Phage encoding peptide inhibitors can be selected by selecting for phage that bind specifically to a PDZ domain protein and/or to an NS1 PL. Libraries are generated fused to proteins such as gene II that are expressed on the surface of the phage. The libraries can be composed of peptides of various lengths, linear or constrained by the inclusion of two Cys amino acids, fused to the phage protein or can also be fused to additional proteins as a scaffold. One can also design libraries biased toward the PL regions disclosed herein or biased toward peptide sequences obtained from the selection of binding phage from the initial libraries provide additional test inhibitor compound.
The NS1, NS1 PL, PDZ and PDZ PL binding domain polypeptides of the invention are useful for generating antibodies for use in diagnostics and therapeutics. The antibodies can be polyclonal antibodies, distinct monoclonal antibodies or pooled monoclonal antibodies with different epitopic specificities. Monoclonal antibodies are made from antigen-containing fragments of the protein by standard procedures according to the type of antibody (see, e.g., Kohler, et al., Nature, 256:495, (1975); and Harlow & Lane, Antibodies, A Laboratory Manual (C.S.H.P., NY, 1988) Queen et al., Proc. Natl. Acad. Sci. USA 86:10029-10033 (1989) and WO 90/07861; Dower et al., WO 91/17271 and McCafferty et al., WO 92/01047 (each of which is incorporated by reference for all purposes). Phage display technology can also be used to mutagenize CDR regions of antibodies previously shown to have affinity for the peptides of the present invention. Some antibodies bind to an epitope present in one form of NS1 or PDZ protein but not others. For example, some antibodies bind to an epitope within the C-terminus PL site of NS1. Those antibodies that bind to specific NS1 PL motifs can be classified as NS1 PL class-specific antibodies. Further, some antibodies bind to an epitope within the PDZ domain of a PDZ protein. Some antibodies specifically bind to a PDZ polypeptide such as that shown in Table 1 without binding to others. The antibodies can be purified, for example, by binding to and elution from a support to which the polypeptide or a peptide to which the antibodies were raised is bound.
The term “antibody” or “immunoglobulin” is used to include intact antibodies and binding fragments thereof. Typically, fragments compete with the intact antibody from which they were derived for specific binding to an antigen fragment including separate heavy chains, light chains Fab, Fab′ F(ab′)2, Fabc, and Fv. Fragments are produced by recombinant DNA techniques, or by enzymatic or chemical separation of intact immunoglobulins. The term “antibody” also includes one or more immunoglobulin chains that are chemically conjugated to, or expressed as, fusion proteins with other proteins. The term “antibody” also includes bispecific antibody. A bispecific or bifunctional antibody is an artificial hybrid antibody having two different heavy/light chain pairs and two different binding sites. Bispecific antibodies can be produced by a variety of methods including fusion of hybridomas or linking of Fab′ fragments. See, e.g., Songsivilai & Lachmann, Clin. Exp. Immunol. 79:315-321 (1990); Kostelny et al., J. Immunol. 148, 1547-1553 (1992).
The antibodies may be utilized as reagents (e.g., in pre-packaged kits) for prognosis and diagnosis of influenza A infection and subtypes thereof, and in particular Avian influenza A infection. A variety of methods may be used to prognosticate and diagnose influenza A infection.
A. Pan-Reactive Antibodies
Pan-reactive or pan-specific antibodies are monoclonal or polyclonal antibodies that bind to any and all influenza A virus NS1 proteins or alternatively, that bind to more than 3 influenza NS1 proteins, or more preferably more than 5. Preferably, the pan-reactive or Pan-specific antibodies recognize at least the following three influenza A strains: H5N1, H3N2, and H1N1. Pan-reactive antibodies can be used to identify the presence of an influenza A virus without identifying what subtype it is. Thus, pan-reactive monoclonal antibodies can specifically recognize conserved regions of the NS1 protein or can recognize two or more PL regions of the NS1 proteins or specific NS1 PL classes. Preferred conserved regions of the NS1 protein can be found for example in the RNA binding domain and are shown on the National Center for Biotechnology Information website as NCBI IVNS1ABP. While, the PL region has been shown to differ between virus subtypes, it is possible to identify monoclonal antibodies that bind to more than one PL in the NS1 region.
However, other embodiments of pan-reactive antibodies include polyclonal antibodies and/or mixtures of monoclonal antibodies that, as a whole, identify all or many influenza A viruses. These antibodies can recognize conserved or non-conserved regions of the NS1 protein. If the antibodies recognize the NS1 PL region, the mixture of antibodies preferably recognize the NS1 that also contain PL regions: ESEV (SEQ ID NO:2), ESEI (SEQ ID NO:3), ESKV (SEQ ID NO:4), TSEV (SEQ ID NO:5), GSEV (SEQ ID NO:6), RSEV (SEQ ID NO:7), RSKV (SEQ ID NO:8), GSEI (SEQ ID NO:9), GSKV (SEQ ID NO:10), NICI (SEQ ID NO:11), TICI (SEQ ID NO:12), RICI (SEQ ID NO:13), DMAL (SEQ ID NO:14), DMTL (SEQ ID NO:15), DIAL (SEQ ID NO:16), DLDY (SEQ ID NO:17), SICL (SEQ ID NO:18), SEV, SEI, SKV and SKI. If more than one antibody and/or PDZ protein is used, the PDZ protein is preferably at least one of those selected from Tables 1 or 2 and the antibody preferably mimics at least one of the PDZ proteins.
B. Monoclonal Antibody Surrogates of PDZ Proteins
As shown above and in the examples, there are a wide variety of PDZ proteins that recognize and bind to the PL motif on NS1 proteins. Antibodies that recognize the same motif can also be used as surrogates of these PDZ proteins. Preferably, the PDZ proteins are one of the following: Outer membrane proteins, PSD95 (PDZ #2); PSD95 (PDZ #1,2,3); DLG1 (PDZ #1); DLG1 (PDZ #1,2); DLG1 (PDZ #2); DLG2 (PDZ #1); DLG2 (PDZ #2); Magi3 (PDZ #1); PTN3 (PDZ #1); MAST2 (PDZ #1); NeDLG (PDZ #1,2); Shank1 d1; Shank2 d1; Shank3 d1; Syntrophin1 alpha; Syntrophin gamma 1; Magi1 (PDZ #1); Magi1 (PDZ #4); Tip1; PTPL1 (PDZ #1); Mint3 (PDZ #1); Lym Mystique (PDZ #1); DLG2 (PDZ #3); MUPP1 (PDZ #8); NeDLG (PDZ #1); DLG5 (PDZ #1); PSD95 (PDZ #1); NumBP (PDZ #3); LIMK1 (PDZ #1); KIAA0313; DLG1 (PDZ #2); Syntenin (PDZ #2); Pick1 or an analog or fragment. More preferably the antibodies mimic any PDZ protein that specifically recognizes the PL ESEV (SEQ ID NO:2). The antibodies surrogates that recognize specific NS1 PL motifs can be designated NS1 PL class-specific.
C. Mixture of Antibodies and Other Binding Agents
A mixture of antibodies and PDZ proteins (and/or aptamers) can be used in any of the assays. The PDZ proteins and antibodies can be used for identification of different sub-types of NS1, identification of influenza A virus, and identification of pathogenic forms as compared to those that are less pathogenic. In some assays, the antibody(s) and PDZ protein(s) are mixed and administered together to a sample. In other assays, the antibody(s) and PDZ protein(s) are separated and allowed to bind to different samples for identification of two different subtypes or for confirmation of the identification of a subtype.
Aptamers are RNA or DNA molecules selected in vitro from vast populations of random sequence that recognize specific ligands by forming binding pockets. Allosteric ribozymes are RNA enzymes whose activity is modulated by the binding of an effector molecule to an aptamer domain, which is located apart from the active site. These RNAs act as precision molecular switches that are controlled by the presence or absence of a specific effector. Aptamers can bind to nucleic acids, proteins, and even entire organisms. Aptamers are different from antibodies, yet they mimic properties of antibodies in a variety of diagnostic formats. Thus, aptamers can be used instead of or in combination with antibodies and/or PDZ proteins to identify the presence of general and specific NS1 PL regions.
The nonstructural proteins NS1 and NS2 of Influenza A are both produced from the same gene using differential splicing. The type of splicing that occurs results in differences at the carboxy terminus of the NS1 and NS2 proteins. In the case of NS1 this results in the distinctive PL at the carboxy terminus, whereas NS2 does not possess a PL at the C-terminus. Because the specific sequence of the PL region in NS1 can be correlated with pathogenicity, changes in the NS2 protein were analyzed for any type of correlation. The NS2 sequences resulting from the splice were analyzed in pathogenic strains as compared to those that were not pathogenic. The sequence was analyzed both at the protein level and at the nucleotide level in Tables 12 and 13. The tables show that a Glycine to Serine substitution in position 70 is highly correlative with the pathogenicity and/or virulence of the virus, particularly with reference to the H1N1 strain that of 1918. An exemplary NS2 sequence is described by the H5N1 strain as described by the National Center for Biotechnology Information (www.ncbi.gov) for example AF144307, and the amino acid and codons encoding the amino acids are numbered for other NS2 proteins correspondingly when the sequences are maximally aligned. Because of this correlation, a method was identified that uses the NS2 polymorphism at Ser 70 as a separate test to analyze whether a given influenza A strain is pathogenic. The method may also be used to identify specific Influenza strains. Alternatively, the NS2 polymorphism can be used in conjunction with the NS1 tests disclosed herein to identify pathogenicity or to confirm pathogenicity identified by a different method.
Methods of screening for the Ser 70 sequence change in the NS2 protein include methods of identifying the change at the protein level or at the nucleotide level.
1. Protein-Based Diagnostic Tests
The invention provides protein-based diagnostic tests to identify the presence of an NS2 protein comprising Ser 70 for identifying Influenza A viruses, Influenza A virus strains, and pathogenic Influenza A virus strains. The diagnostic tests using the Ser 70 polymorphic sequence in NS2 can use the same formats as those for use in NS1 analysis (see section VIII and other related sections). The assay identifies the presence of a serine at position 70 and if the serine is present, the influenza strain is identified as pathogenic. If the serine is not present, the influenza strain is identified as not pathogenic.
Monoclonal or polyclonal antibodies that recognize the Serine 70 change in the NS2 protein can be used to identify an influenza strain as Influenza A, can identify a specific Influenza A strain, and can identify whether a virus is pathogenic. NS2 antibodies can be produced to recognize the presence of a Serine 70 and can be used to identify pathogenic strains. For example, antibodies can be produced using the peptides provided in Tables 12 or 13 for the NS2 region having a serine at the 70 position. Ser-70 antibodies can then be screened to ascertain whether they cross-react with a peptide having a Glycine or other amino acid at position 70. Alternatively, the antibodies can be produced to recognize the specific sequence comprising the Serine 70 for each strain, producing strain-specific antibodies (see also section VIII as applied to NS1 antibodies). In some assays, the antibody is used to identify a strain as pathogenic. In some assays the NS2 antibody is used as an alternative to an NS1 antibody. In some assays the NS2 antibody is used in combination with an NS1 antibody in any of the assays employing the NS1 protein. The NS2 antibody can be used to identify a specific Influenza A virus, to identify a virus as an Influenza A virus, or to identify a virus as pathogenic.
Alternatively, other binding agents can be used in lieu of antibodies, such as peptides selected by phage display library techniques.
2. Nucleic Acid Diagnostic Tests
The invention also provides nucleic acid-based diagnostic tests to identify the presence of an NS2 nucleic acid coding for a protein comprising Ser 70. These can be used for identifying Influenza A viruses, Influenza A virus strains, and pathogenic Influenza A virus strains. The diagnostic tests use a sequence comprising a codon encoding the Ser 70 in NS2 in a variety of formats. For example, the diagnostic tests can use probes or primers complementary to a sequence encoding the Ser 70. Preferably, the sequences encoding the peptides identified in Table 12 are used. If the Ser 70 is identified as present, the influenza virus is identified as pathogenic.
Methods of detection of polymorphisms in NS2. The identity of bases occupying the sequence comprising Ser-70 shown in Table 12 of the NS2 nucleic acid can be determined in a sample by several methods, which are described in turn.
A. Single Base Extension Methods
Single base extension methods are described by e.g., U.S. Pat. No. 5,846,710, U.S. Pat. No. 6,004,744, U.S. Pat. No. 5,888,819 and U.S. Pat. No. 5,856,092. In brief, the methods work by hybridizing a primer that is complementary to a target sequence such that the 3′ end of the primer is immediately adjacent to but does not span a site of potential variation in the target sequence. That is, the primer comprises a subsequence from the complement of a target polynucleotide terminating at the base that is immediately adjacent and 5′ to the polymorphic site. The hybridization is performed in the presence of one or more labeled nucleotides complementary to base(s) that may occupy the site of potential variation. For example, for the sequence encoding the NS2 Ser 70 polymorphisms, one or more labeled nucleotides primers can be used. The primers for each polymorphism can include different labels to differentiate the polymorphism. Preferably, the primer overlaps or partially codes for the splicing site. This means that some part of the splicing site or polymorphic region is contained in the primer, preferably the Ser 70 site. In some methods, particularly methods employing multiple differentially labeled nucleotides, the nucleotides are dideoxynucleotides. Hybridization is performed under conditions permitting primer extension if a nucleotide complementary to a base occupying the site of variation in the target sequence is present. Extension incorporates a labeled nucleotide thereby generating a labeled extended primer. If multiple differentially labeled nucleotides are used and the target is heterozygous then multiple differentially labeled extended primers can be obtained. Extended primers are detected providing an indication of which bas(es) occupy the site of variation in the target polynucleotide.
B. Allele-Specific Probes
The design and use of probes for analyzing polymorphisms is described by e.g., Saiki et al., Nature 324, 163-166 (1986); Dattagupta, EP 235,726, Saiki, WO 89/11548. Using this disclosure, probes can be designed that recognize specific sequences comprising the Ser 70 polymorphism that hybridizes to a segment of target DNA from one type of virus or viral strain but do not hybridize to the corresponding segment from another type of virus or viral strain due to the presence of different polymorphic forms in the respective segments from the two viruses. Hybridization conditions should be sufficiently stringent that there is a significant difference in hybridization intensity between alleles of the Ser 70 region, and preferably an essentially binary response, whereby a probe hybridizes to only one of the alleles. Some probes are designed to hybridize to a segment of target DNA such that the polymorphic site at Ser 70 aligns with a central position (e.g., in a 15 mer at the 7 position; in a 16 mer, at either the 8 or 9 position) of the probe. This design of the probe achieves good discrimination in hybridization between different nucleic acids encoding NS2 proteins from different viruses and/or strains.
These probes are often used in pairs, one member of a pair showing a perfect match to one reference form of a target sequence and the other member showing a perfect match to a variant form or a different reference form. Several pairs of probes can then be immobilized on the same support for simultaneous analysis of multiple polymorphisms within the same target sequence. The polymorphisms can also be identified by hybridization to nucleic acid arrays, some example of which are described by WO 95/11995 (incorporated by reference in its entirety for all purposes).
C. Allele-Specific Amplification Methods
An allele-specific primer hybridizes to a site on target DNA overlapping a polymorphism and only primes amplification of an allelic form to which the primer exhibits perfect complementarily. See Gibbs, Nucleic Acid Res. 17, 2427-2448 (1989). This primer is used in conjunction with a second primer that hybridizes at a distal site. Amplification proceeds from the two primers leading to a detectable product signifying the particular allelic form is present. A control is usually performed with a second pair of primers, one of which shows a single base mismatch at the polymorphic site and the other of which exhibits perfect complementarily to a distal site. The single-base mismatch prevents amplification and no detectable product is formed. In some methods, the mismatch is included in the 3′-most position of the oligonucleotide aligned with the polymorphism because this position is most destabilizing to elongation from the primer. See, e.g., WO 93/22456. In this case, the allele specific primer can be designed to overlap the splice site of NS2, comprising the Ser 70 position.
D. Direct-Sequencing
The direct analysis of the sequence of the NS2 polymorphisms of the present invention can be accomplished using either the dideoxy-chain termination method or the Maxam-Gilbert method (see Sambrook et al., Molecular Cloning, A Laboratory Manual (2nd Ed., CSHP, New York 1989); Zyskind et al., Recombinant DNA Laboratory Manual, (Acad. Press, 1988)).
E. Denaturing Gradient Gel Electrophoresis
Amplification products generated using the polymerase chain reaction can be analyzed by the use of denaturing gradient gel electrophoresis. Different alleles of the NS2 Ser 70 polymorphism can be identified based on the different sequence-dependent melting properties and electrophoretic migration of DNA in solution. Erlich, ed., PCR Technology, Principles and Applications for DNA Amplification, (W.H. Freeman and Co, New York, 1992), Chapter 7.
F. Single-Strand Conformation Polymorphism Analysis
Alleles of target sequences can be differentiated using single-strand conformation polymorphism analysis, which identifies base differences by alteration in electrophoretic migration of single stranded PCR products, as described in Orita et al., Proc. Nat. Acad. Sci. 86, 2766-2770 (1989). Amplified PCR products can be generated as described above, and heated or otherwise denatured, to form single stranded amplification products. Single-stranded nucleic acids may refold or form secondary structures that are partially dependent on the base sequence. The different electrophoretic mobilities of single-stranded amplification products can be related to base-sequence difference between alleles of target sequences.
3. NS2 therapeutics—the anti-viral therapeutics and methods for screening for anti-viral therapeutics as disclosed herein for NS1 and PDZ proteins can similarly be used for identifying binding partners for NS2, identifying therapeutic agents that block an interaction between NS2 and a binding partner, and treating a patient with a virulent Influenza A infection. (see section XI Pharmaceutical compositions). Targets for identification of therapeutics with respect to NS2 include agents that block the interaction between a binding partner and NS2 at the overlap region, including the Ser 70, agents that block an interaction between an NS2 PL (internal site) and a PDZ binding partner, and serine kinases that phosphorylate the Ser 70, resulting in inhibition of an interaction.
Embodiments of the invention provide diagnostic capture and detect reagents useful in assay methods for identifying influenza A viruses and their products in a variety of different types of biological samples. Representative assay formats useful for detecting influenza viruses include enzyme-linked solid-phase absorbent assays, radiolabeled binding assays, fluorescence PDZ- and PL-binding assays, time-resolved PDZ and PL fluorescence assays, as well as, sandwich- and enzyme-cascade assay formats. Illustrative methods, as may be adaptable from the immunoassay art for use in the subject assays include homogeneous and heterogeneous assay formats; competitive and non-competitive assay formats; enzyme-linked solid phase assay formats, fluorescence assay formats, time resolved fluorescence assay formats, bioluminescent assay formats, cascade enzyme assays and the like.
In certain embodiments of the invention, one or more PDZ proteins are used as capture agents to isolate one or more PL analyte from a biological sample. In other alternative embodiments, one or more PDZ proteins are conjugated with one or more signal generating compounds and used as detect reagents for identifying the presence or amount of one or more PL analytes in a biological sample. In yet other embodiments, PL proteins and PL peptides are conjugated with signal generating compounds (PL-SGC) and used in competitive ligand inhibition assays, i.e., where the presence of a viral PL competes the binding of one or more PL-SGC to a PDZ. Preferably, the PDZ proteins are at least one of: Outer membrane protein, PSD95 (PDZ #2); PSD95 (PDZ #1,2,3); DLG1 (PDZ #1); DLG1 (PDZ #1,2); DLG1 (PDZ #2); DLG2 (PDZ #1); DLG2 (PDZ #2); Magi3 (PDZ #1); PTN3 (PDZ #1); MAST2 (PDZ #1); NeDLG (PDZ #1,2); Shank1 d1; Shank2 d1; Shank3 d1; Syntrophin1 alpha; Syntrophin gamma 1; Magi1 (PDZ #1); Magi1 (PDZ #4); Tip1; PTPL1 (PDZ #1); Mint3 (PDZ #1); Lym Mystique (PDZ #1); DLG2 (PDZ #3); MUPP1 (PDZ #8); NeDLG (PDZ #1); DLG5 (PDZ #1); PSD95 (PDZ #1); NumBP (PDZ #3); LIMK1 (PDZ #1); KIAA0313; DLG1 (PDZ #2); Syntenin (PDZ #2); Pick1 or an analog or fragment. For tests that generally identify influenza A, a mixture of PDZ proteins and antibodies can be used. For these tests, the PDZ protein may include one of the above in admixture with others that recognize other pathogen-specific or influenza A specific PL motifs.
The present invention provides methods of detecting pathogen PL proteins in a sample and finds utility in diagnosing viral infection in a subject. In many embodiments, a biological sample is obtained from a subject, and, the presence of a pathogen PL protein in the sample is determined. The presence of a detectable amount of pathogen PL protein in a sample indicates that the individual is infected with a particular virus. In other embodiments, the level of pathogen PL protein in a biological sample is determined, and compared to the amount of a control in the sample. The relative amount of pathogen PL protein in a sample indicates the severity of the infection by the pathogen.
The methods generally involve two binding partners specific for an influenza A PL protein, one of which is a PDZ domain polypeptide, as described above. In general, the methods involve a) isolating the pathogen PL from a sample using one of the binding partners, and b) detecting the pathogen PL protein with the other binding partner.
For sub-type specific tests or NS1 PL class-specific tests, the PL to be identified is preferably one of: ESEV (SEQ ID NO:2), ESEI (SEQ ID NO:3), ESKV (SEQ ID NO:4), TSEV (SEQ ID NO:5), GSEV (SEQ ID NO:6), RSEV (SEQ ID NO:7), RSKV (SEQ ID NO:8), GSEI (SEQ ID NO:9), GSKV (SEQ ID NO:10), NICI (SEQ ID NO:11), TICI (SEQ ID NO:12), RICI (SEQ ID NO:13), DMAL (SEQ ID NO:14), DMTL (SEQ ID NO:15), DIAL (SEQ ID NO:16), DLDY (SEQ ID NO:17), SICL (SEQ ID NO:18), SEV, SEI, SKV and SKI. For the sub-type specific test, the PDZ protein used is preferably at least one of: PSD95 (PDZ #2); PSD95 (PDZ #1,2,3); DLG1 (PDZ #1); DLG1 (PDZ #1,2); DLG1 (PDZ #2); DLG2 (PDZ #1); DLG2 (PDZ #2); Magi3 (PDZ #1); PTN3 (PDZ #1); MAST2 (PDZ #1); NeDLG (PDZ #1,2); Shank1 d1; Shank2 d1; Shank3 d1; Syntrophin1 alpha; Syntrophin gamma 1; Magi1 (PDZ #1); Magi1 (PDZ #4); Tip1; PTPL1 (PDZ #1); Mint3 (PDZ #1); Lym Mystique (PDZ #1); DLG2 (PDZ #3); MUPP1 (PDZ #8); NeDLG (PDZ #1); DLG5 (PDZ #1); PSD95 (PDZ #1); NumBP (PDZ #3); LIMK1 (PDZ #1); KIAA0313; DLG1 (PDZ #2); Syntenin (PDZ #2); Pick1 or an analog or fragment. The NS1 PL can be strongly predictive of the H and A antigens and sub-type of the virus.
For the pathogen-specific test, the NS1 PL to be identified is preferably at least one of: ESEV (SEQ ID NO:2), ESEI (SEQ ID NO:3), ESKV (SEQ ID NO:4), TSEV (SEQ ID NO:5), GSEV (SEQ ID NO:6), RSEV (SEQ ID NO:7), RSKV (SEQ ID NO:8), GSEI (SEQ ID NO:9), GSKV (SEQ ID NO:10), NICI (SEQ ID NO:11), TICI (SEQ ID NO:12), RICI (SEQ ID NO:13), DMAL (SEQ ID NO:14), DMTL (SEQ ID NO:15), DIAL (SEQ ID NO:16), DLDY (SEQ ID NO:17), SICL (SEQ ID NO:18), SEV, SEI, SKV and SKI. For the pathogen-specific test, the PDZ protein used is preferably at least one of those selected from Tables 1 or 2 or an analog or fragment.
For the influenza A specific test, the NS1 PL to be identified is preferably at least one of: ESEV (SEQ ID NO:2), ESEI (SEQ ID NO:3), ESKV (SEQ ID NO:4), TSEV (SEQ ID NO:5), GSEV (SEQ ID NO:6), RSEV (SEQ ID NO:7), RSKV (SEQ ID NO:8), GSEI (SEQ ID NO:9), GSKV (SEQ ID NO:10), NICI (SEQ ID NO:11), TICI (SEQ ID NO:12), RICI (SEQ ID NO:13), DMAL (SEQ ID NO:14), DMTL (SEQ ID NO:15), DIAL (SEQ ID NO:16), DLDY (SEQ ID NO:17), SICL (SEQ ID NO:18), SEV, SEI, SKV and SKI. For the pathogen-specific test, the PDZ protein used is preferably at least one of those selected from Tables 1 or 2 or an analog or fragment.
A. ELISA Sandwich Heterogeneous Assay Format
Using the instant PDZ capture and monoclonal anti-NS1, as illustrated in the Examples section, below, a sandwich assay format was constructed to detect high risk influenza A strains in biological samples. The instant assays had a sensitivity in the range of 1-1,000 ng/ml, i.e., sufficiently sensitive for commercial use in detecting the type or amount of an Influenza A virus in a biological sample, with the following caveats: namely,
While a variety of competitive and non-competitive assay formats are identifiable for possible use in the instant methods, a sandwich assay format is presently preferred because these assays have proven performance characteristics and a variety of well established signal amplification strategies. In a presently preferred sandwich immunoassay embodiment, a specific high affinity non-natural PDZ protein is employed to capture a natural viral NS1 antigen from within a biological sample; an anti-NS1 mouse monoclonal antibody is used to detect the bound NS1 antigen; and, the presence of the bound anti-NS1 antibody is detected using a signal generating compound, e.g. with either an enzyme-conjugated second antibody (e.g., horse radish peroxidase-conjugated antibody; HRP) or a biotinylated second antibody and streptavidin-enzyme conjugate (e.g., HRP).
In general, methods of the invention involve the steps of (i) separating (i.e., isolating) native viral PL protein analyte from within a complex biological sample using a first binding agent, i.e., a capture agent; and, (ii) detecting the isolated PL analyte using a second binding agent, i.e., a detect agent. The separating and detecting steps may be achieved using binding partners that have different levels of specificity for the PL analyte, e.g., if the capture agent is highly specific then lesser specificity may be required in the detect reagent and vice versa. In certain embodiments, the capture agent is preferably a PDZ domain polypeptide. More preferably, the capture agent is one of those listed in Table 1 and/or Table 2. In alternative embodiments, the first binding partner is an anti-pathogen PL protein antibody or mixture of antibodies, with the proviso that in these embodiments at least one component of the detect reagent is a PDZ polypeptide, e.g., a PDZ protein detect agent that binds to the captured/isolated PL analyte and whose presence in the complex is then detected using an anti-PDZ antibody conjugated with a signal generating compound. In certain presently preferred embodiments, a PDZ capture agent is bound, directly or via a linker, to a solid phase. For example, in one non-limiting example the PDZ domain polypeptide is bound to a magnetic bead. In the latter example, when brought into contact with a biological sample the PDZ capture agent immobilized on the magnetic bead is effective in forming a PDZ-PL interaction complex with an influenza virual PL protein in the sample. Next, a magnetic field is applied and the interaction complex, with the bound influenza virus PL, is isolated from the sample. In another non-limiting example, a PDZ domain polypeptide capture agent is immobilized on the surface of a microtiter plate; a biological sample containing an influenza PL is brought into contact with the immobilized capture reagent resulting in binding of the PL to the surface of the plate; the plate is washed with buffer removing non-PL viral analytes from the plate; and, the immobilized PL analyte is, thus, isolated from the biological sample. Different separation/isolation means are known, e.g., applying a magnetic field, washing and the like. The particular means employed is dependent upon the particular assay format. For example, separation may be accomplished by a number of different methods including but not limited to washing; magnetic means; centrifugation; filtration; chromatography including molecular sieve, ion exchange and affinity; separation in an electrical field; capillary action as e.g. in lateral flow test strips; immunoprecipitation; and, the like as disclosed further below.
In certain embodiments, influenza PL protein is separated from other viral and cellular proteins in a biological sample by bringing an aliquot of the biological sample into contact with one end of a test strip, and then allowing the proteins to migrate on the test strip, e.g., by capillary action such as lateral flow. The instant methods are distinguished from prior immunoassay methods by the presence in the assay of one or more PDZ polypeptide agents, antibodies, and/or aptamers, e.g., as capture and/or detect reagents, conferring upon the assay the ability to specifically identify the presence or amount of a high risk influenza A strain of virus. The instant methods are distinguished from prior immunoassay methods by the fact that they identify a viral protein that is present in the patient sample, rather than an antibody. Methods and devices for lateral flow separation, detection, and quantification are known in the art, e.g., U.S. Pat. Nos. 6,942,981, 5,569,608; 6,297,020; and 6,403,383 incorporated herein by reference in their entirety. In one non-limiting example, a test strip comprises a proximal region for loading the sample (the sample-loading region) and a distal test region containing a PDZ polypeptide capture agent and buffer reagents and additives suitable for establishing binding interactions between the PDZ polypeptide and any influenza PL protein in the migrating biological sample. In alternative embodiments, the test strip comprises two test regions that contain different PDZ domain polypeptides, i.e., each capable of specifically interacting with a different influenza PL protein analyte.
According to the methods disclosed above, influenza PL protein analytes are separated from other proteins in a biological sample, i.e., in such a manner that the analyte in the sample is suitable for detection and/or quantification. Embodiments of the invention provide novel methods for detection of isolated influenza PL proteins using PDZ polypeptides, PDZ polypeptides conjugated with signal generating compounds, antibodies, aptamers and the like. According to alternative embodiments, influenza PL analyte bound to a PDZ capture agent, antibody and/or aptamer is detected using an antibody or antibodies specific for the pathogen PL protein, e.g., an antibody conjugated with a signal generating compound. A variety of detection methods are, of course, known in the diagnostic arts and it is not the intention of the present (non-limiting) disclosure to set forth all alternative well-known methods. Rather, the instant disclosure is intended to satisfy the requirement for setting forth the best mode of practicing the invention and to act as a general reference guide to alternative methods.
In certain embodiments, a PDZ domain conjugated with an SGC (signal generating compound) is used to detect the presence of a pathogen PL protein analyte in a sample in a homogeneous assay format, i.e., without need for a separation step. In this assay method the binding of a PL to the PDZ domain induces a change in the signal produced by the SGC, e.g., a change in fluorescent anisotropy.
In other embodiments, heterogeneous solid phase assay formats (disclosed supra) are useful for detecting influenza PL analytes in biological samples. As noted in the Background section above, PDZ proteins bind cellular proteins containing PL. Similarly, in infected cells influenza viral proteins containing PL bind host cell PDZ proteins. While these interactions would normally be expected to compete with binding in a diagnostic assay format, further guidance is provided hereby that, unexpectedly, the affinities and mass balance of these latter natural interactions are sufficiently weak, or are sufficiently disrupted in detergent extracted cell lysates, that influenza PL analytes are detectable in the instant diagnostic assay formats. Accordingly, lysates may be prepared and assays may be conducted in the presence of less than about 0.5% of a detergent such as Tween-20 or Triton X100; preferably, less than about 0.2%; and, most preferably, less than about 0.1%.
In certain embodiments, the level of viral PL protein in a sample may be quantified and/or compared to controls. Suitable negative control samples are e.g. obtained from individuals known to be healthy, e.g., individuals known not to have a influenza viral infection. Specificity controls may be collected from individuals having known influenza B infection, or individuals infected with lower virulence influenza strains, e.g., H1N1, H3N2 and the like. Control samples can be from individuals genetically related to the subject being tested, but can also be from genetically unrelated individuals. A suitable negative control sample may also be a sample collected from an individual at an earlier stage of infection, i.e., a time point earlier than the time point at which the test sample is taken. Embodiments of the invention also include non-infectious positive controls, i.e., recombinant proteins having amino acid sequences of high-risk influenza A viral PL.
Initial Western blots, (see the Examples section, below), show that NS1 levels in biological samples are sufficient to allow detection of these antigens in a variety of different possible immunoassay formats. However, should the levels of NS1 in a particular biological sample prove to be limiting for detection in a particular immunoassay format, then, as one other alternative embodiment, the live virus in a biological sample can be amplified by infecting cells in vitro, i.e., the NS1 protein in the virus-amplified sample should be detectable in about 6 hrs to about 12 hrs. In other alternative embodiments, methods for improving the yield of NS1 antigen in biological samples and virus-amplified samples include uses of protease inhibitors and proteasome inhibitors, e.g. MG132.
B. Preparation of Reagents
PL peptides, PDZ domain polypeptides, and aptamers may be made synthetically (i.e., using a machine) or using recombinant means, as is known in the art. For example, methods and conditions for expression of recombinant proteins are well known in the art, e.g., see Sambrook, supra, and Ausubel, supra. The use of mammalian tissue cell culture to express polypeptides is discussed generally in Winnacker, “From Genes to Clones, VCH Publishers, N.Y., N.Y., 1987; and, in Ausubel, supra.
Details of the binding assays are also disclosed in U.S. patent application Ser. No. 10/630,590, filed Jul. 29, 2003 and published as US20040018487 and in U.S. Pat. No. 6,942,981.
Cell-based assays generally involve co-producing (i.e., producing in the same cell, regardless of the time at which they are produced), the subject PDZ domain polypeptides and influenza PL using recombinant expression systems. Suitable cells for producing the subject polypeptides in eukaryotic cells are disclosed in the Examples section, below. Cell types that are potentially suitable for expression of subject PDZ domain polypeptide and influenza PL include the following: e.g., monkey kidney cells (COS cells), monkey kidney CVI cells transformed by SV40 (COS-7, ATCC CRL 165 1); human embryonic kidney cells (HEK-293, Graham et al. J. Gen Virol. 36:59 (1977)); HEK-293T cells; baby hamster kidney cells (BHK, ATCC CCL 10); Chinese hamster ovary-cells (CHO, Urlaub and Chasin, Proc. Natl. Acad. Sci. (USA) 77:4216, (1980); mouse sertoli cells (TM4, Mather, Biol. Reprod. 23:243-251 (1980)); monkey kidney cells (CVI ATCC CCL 70); african green monkey kidney cells (VERO-76, ATCC CRL-1587); human cervical carcinoma cells (HELA, ATCC CCL 2); canine kidney cells (MDCK, ATCC CCL 34); buffalo rat liver cells (BRL 3A, ATCC CRL 1442); human lung cells (W138, ATCC CCL 75); human liver cells (hep G2, HB 8065); mouse mammary tumor (MMT 060562, ATCC CCL 51); TR1 cells (Mather et al., Annals N.Y. Acad. Sci. 383:44-68 (1982)); NIH/3T3 cells (ATCC CRL-1658); and mouse L cells (ATCC CCL-1). Additional cell lines will be apparent. A wide variety of cell lines are available from the American Type Culture Collection, 10801 University Boulevard, Manassas, Va. 20110-2209.
C. Sample Preparation
Any sample can be used that contains a detectable concentration of influenza proteins and preferably of NS1. Examples of samples that can be used are lung exudates, cell extracts (respiratory, epithelial lining nose), blood, mucous, and nasal swabs, for example. It was shown herein that a very high concentration of NS1 can be found in nasal swabs from swine and humans. This was surprising in that NS1 was thought to be an intracellular protein. Thus, a preferred sample for identification of NS1 is nasal secretion.
Binding of the PL protein to the PDZ protein and/or to an antibody was shown in the Examples to occur in the presence of up to 0.05% SDS, including 0.03% and 0.01%. Therefore, when the nasal or other bodily secretion is not likely to easily be used in a lateral flow format, it can be treated with SDS. Preferably, the amount of SDS added is up to a final concentration of 0.01%, more preferably 0.03% and even more preferably, 0.05%. Other detergents and the like can be used that do not interfere with binding of the PDZ protein, antibody, or aptamer or other agent to the PL protein. Other methods of sample treatment that do not interfere with protein/protein interactions can be used, including dilution with a buffer or water.
D. General Influenza A Test Alone or in Combination
This test identifies the presence of influenza A in a sample. Therefore, the test can use the method of identifying the presence of an NS1 conserved region using antibodies or aptamers or the like. Preferably, a single monoclonal antibody or a single aptamer identifies all of the variants of NS1. This is most likely when using an antibody that recognizes a conserved region of the NS1 protein. Alternatively, more than one antibody and/or aptamer and/or PDZ protein or other binding agent can be uses to identify all Influenza A subtypes. The method can also use a mixture of antibodies and PDZ proteins to identify all influenza A by the presence of the NS1 protein. The general Influenza A test can be used in combination with a more specific test to subtype the virus, the tests can be performed sequentially or at the same time. See also the description of a pan-specific antibody above for preferred PL regions and PDZ proteins if used in the test.
E. Pathogenic Influenza A Test
This test identifies all forms of the virus having an NS1 protein PL motif. It was identified herein that the nonpathogenic strains of Influenza A have NS1 proteins that are devoid of the avian PL motifs. Thus, methods to specifically identify the presence of a pathogenic influenza A virus can identify the presence of NS1 containing an avian PL region. One or more PDZ proteins and/or antibodies can be used to identify all of the varieties of PL regions. For example, if only PDZ proteins were used, at least two PDZ proteins would be necessary to identify all of the NS1 PL proteins. Alternatively, a single antibody that is capable of recognizing NS1 proteins having a PL region is used. Preferably, the PL region of NS1 to be identified is at least one of: ESEV (SEQ ID NO:2), ESEI (SEQ ID NO:3), ESKV (SEQ ID NO:4), TSEV (SEQ ID NO:5), GSEV (SEQ ID NO:6), RSEV (SEQ ID NO:7), RSKV (SEQ ID NO:8), GSEI (SEQ ID NO:9), GSKV (SEQ ID NO:10), NICI (SEQ ID NO:11), TICI (SEQ ID NO:12), RICI (SEQ ID NO:13), DMAL (SEQ ID NO:14), DMTL (SEQ ID NO:15), DIAL (SEQ ID NO:16), DLDY (SEQ ID NO:17), SICL (SEQ ID NO:18), SEV, SEI, SKV and SKI. Preferably, the PL region to be identified is that having an avian PL region. Preferably, the one or more PDZ proteins is at least one of those selected from Tables 1 or 2 or analogs or active fragments.
F. Pathogenic Avian Influenza Virus Type A Test
The NS1 protein from H5N1 avian influenza has a C-terminal sequence that binds to a diversity of PDZ domains that fail to bind to NS1 from typical human influenza, such as H3N2. NS1 protein from 77% of avian flu H5N1 isolates terminates in ESEV (SEQ ID NO:2), moreover, the two most common C-terminal NS1 sequences after ESEV (SEQ ID NO:2), ESKV (SEQ ID NO:4) and ESEI (SEQ ID NO:3), account for another 17% of avian influenza isolates also bind PSD-95 with high affinity (i.e.: 45 nM and 200 nM respectively). H3N2 NS1 terminates in RSKV (SEQ ID NO:8) which binds PSD-95 with very low affinity if at all. Therefore PSD-95 can be used as a detection reagent for avian flu and distinguish avian flu from other strains such as H3N2.
Although any part of PSD-95 protein can be used as long as it has a PDZ domain, PSD-95 domains-1, -2 and -3 have different binding specificities and affinities. As part of the identification of which PDZ protein binds with highest affinity to the avian flu H5N1 PLs (see Example 2 and Tables 4a-e), it was found that the PSD-95 domain 2 PDZ binds with highest affinity. Therefore, the PSD-95 PDZ protein used in the assay need only comprise one PDZ domain from the protein, and preferably comprises at least the PDZ from domain 2 or a fragment thereof sufficient for specific binding. The PSD-95 PDZ protein is contacted with a sample. If the sample contains a pathogenic influenza virus A, the PSD-95 PDZ specifically binds to the PL of the NS1 protein of the pathogenic influenza virus.
A lateral flow format such as that set out in
For qualitative or quantitative analysis and for quality control, any one or all of the following controls can be included. A control band composed of goat anti-mouse antibody (GAM). A lane that identifies whether any influenza A is present, by depositing an antibody that binds to all forms of the NS1 protein on the membrane. A negative control including a PSD-95 protein having all of the domains except the PDZ domains can be included. Other controls can include controls for quantitating the signal, such as purified forms of PL proteins that are known to bind weakly, moderately, strongly or not at all to the capture agent on the membrane, preferably the capture agent is either a PDZ protein or an antibody specific for one or more PLs.
Controls for quantitating the signal can be included to allow for analysis of the strength of binding to differentiate PLs that bind weakly or moderately to PSD-95. For example, Example 6 states the binding strength is quantified by using the following symbols: (−) for no binding, (+) for weak binding, (++) for moderate binding and (+++) for strong binding. The strength of binding to a specific PDZ protein can be used to differentiate H1N1 which has an NS1 that terminates in RSEV which binds PSD-95 with moderate affinity. A positive control for strong binding can be purified NS1 from H5N1, a control for weak binding can be purified NS1 from H3N2, a control for moderate binding can be purified NS1 from H1N1.
Alternatively, other PDZ proteins can be used to further differentiate between strains that bind to PSD-95. For example, as shown in Example 6, both H5N1 and H1N1 bind to PSD-95. So, INADL D8 is used to identify whether the strain is H1N1 or H5N1, since only H1N1 binds. The binding to INADL D8 allows one to unequivocally identify the PL binding to PSD-95 as H5N1. Other PDZ proteins that bind to H1N1 and do not bind to H5N1 can be found in Tables 4a-e and Example 2.
G. Specific NS1 PL Test
This test allows for the identification of a specific class of NS1 PL class by the specific NS1 PL. It may also allow for identifying a subtype by the specific NS1 PL class. Although generally, the type of HA and NP antigens correlate with the NS1 PL region, this is not always the case. It is possible that, for example, due to re-assortment or other genetic processes the virus can undergo, the NS1 PL region from, for example an H1N1 virus can be transferred to an H2N1 virus. However, without being bound to a specific theory, the presence of the NS1 PL region is likely to be more indicative of the pathogenicity of the virus in the patient sample. This may be because of the biological role that NS1 plays in the infection. A preferred test identifies the human PLs ESEV (SEQ ID NO:2). A preferred test identifies the Avian influenza A NS1 PLs having the motifs ESEV (SEQ ID NO:2), ESEI (SEQ ID NO:3), and ESKV (SEQ ID NO:4). This identifies a very pathogenic strain of the virus and appropriate measures can be taken to treat and to contain the disease. Other preferred tests include, for example, an array that allows one to specifically identify the NS1 PL subtype. This type of array can also include a general test for Influenza A. This type of test can also include a test to determine the type of HA and NP protein. Preferably, the PL to be identified is one of: ESEV (SEQ ID NO:2), ESEI (SEQ ID NO:3), ESKV (SEQ ID NO:4), TSEV (SEQ ID NO:5), GSEV (SEQ ID NO:6), RSEV (SEQ ID NO:7), RSKV (SEQ ID NO:8), GSEI (SEQ ID NO:9), GSKV (SEQ ID NO:10), NICI (SEQ ID NO:11), TICI (SEQ ID NO:12), RICI (SEQ ID NO:13), DMAL (SEQ ID NO:14), DMTL (SEQ ID NO:15), DIAL (SEQ ID NO:16), DLDY (SEQ ID NO:17), SICL (SEQ ID NO:18), SEV, SEI, SKV and SKI. More preferably, the NS1 PL to be identified is ESEV (SEQ ID NO:2). Preferably, the at least one PDZ protein used is at least one of those selected from Tables 1 or 2, fragments or analogs. More preferably, the at least one PDZ protein is at least one of: Outer membrane protein, PSD95 (PDZ #2); PSD95 (PDZ #1,2,3); DLG1 (PDZ #1); DLG1 (PDZ #1,2); DLG1 (PDZ #2); DLG2 (PDZ #1); DLG2 (PDZ #2); Magi3 (PDZ #1); PTN3 (PDZ #1); MAST2 (PDZ #1); NeDLG (PDZ #1,2); Shank1 d1; Shank2 d1; Shank3 d1; Syntrophin1 alpha; Syntrophin gamma 1; Magi1 (PDZ #1); Magi1 (PDZ #4); Tip1; PTPL1 (PDZ #1); Mint3 (PDZ #1); Lym Mystique (PDZ #1); DLG2 (PDZ #3); MUPP1 (PDZ #8); NeDLG (PDZ #1); DLG5 (PDZ #1); PSD95 (PDZ #1); NumBP (PDZ #3); LIMK1 (PDZ #1); KIAA0313; DLG1 (PDZ #2); Syntenin (PDZ #2); Pick1 or an analog or fragment and/or antibodies (or aptamers) that mimic any PDZ protein.
H. Test for Serum Antibodies
Tests to identify the presence of serum antibodies that bind to specific NS1 PL motifs and/or to NS2 proteins that have a serine at position 70 can be used in any of the diagnostic methods for formats. The specific NS1 PL peptide and/or peptides that include the overlap region containing the Ser 70 can be used as capture reagents in lateral flow or other formats.
I. Use of the Assay in an Epidemic Setting
Assay sensitivity and specificity can be changed to achieve different absolute levels of detection of influenza A NS1 in a biological sample, e.g., by decreasing the levels of a competitive ligand in a competition assay format, changing the amounts of capture and detect reagent in sandwich assays and the like. Thus, the instant test methods encompass a variety of assays having different performance attributes to meet different needs encountered in different uses as illustrated in the Examples section, below. For instance, in an avian epidemic setting the highest PPV is commonly recorded and positive test results are more likely to be true, i.e., with the lowest NPV and false negative results tending to be more likely. Also in monitoring epidemics of influenza A in avian subjects, it is presently common practice to submit all samples to reference laboratories for testing. By identifying the true positive samples in the instant screening assay, e.g., in the field or at the point of care, the instant test assays find uses in reducing the number of samples that must ultimately be submitted to a reference laboratory for testing, i.e., a particular value when the burden of testing is high during an epidemic. Because it is current practice to slaughter all animals, irrespective of whether they are infected, a relatively high false positive rate may be acceptable, but it must also be accompanied by a relatively low false negative rate. In certain embodiments, the invention provides test kits having different specificity, sensitivity, PPV and NPV for use during epidemics, referred to herein as “epidemic test methods”. Preferably to suit current needs, the instant epidemic test methods have assay performance as follows: namely, specificity greater than about 65%; sensitivity greater than about 90%; PPV greater than about 85%; NPV greater than about 65%; false positive values of less than about 25% and false negative values of less than about 5%.
In contrast, in times of low influenza A incidence in avian subjects, the lowest PPV is commonly recorded with false positive test results more likely and with the highest NPV and negative tests results tending to be more likely and true. During these times of low incidence the aim in screening may be to rapidly identify potentially infected animals and isolate them until confirmatory testing is completed e.g. in a reference laboratory. Thus, in certain embodiments the invention provides test methods having increased sensitivity and NPV for use during times of low influenza A incidence where monitoring is essential, i.e., referred to herein as “monitoring test methods”. Preferably, the instant monitoring test methods have assay performance as follows: namely, specificity greater than about 65%; sensitivity greater than about 90%; PPV greater than about 85%; NPV greater than about 65%; false positive values of less than about 20% and false negative values of less than about 5%. When the instant monitoring test methods are used to screen more than 100 members of a flock, the PPV for the flock as a whole is significantly higher than the predictive values achieved in any one particular assay. Thus, when a positive test result is obtained in a monitoring test method it may prove beneficial to retest the members of the flock using an epidemic test assay, supra.
In human, rather than avian, testing the aims are of course different. Timely evidence of an influenza A infection can have important case management implications, e.g., prompting early administration of anti-viral agents in children or aged subjects. Generally with human diagnostic products a high degree of specificity and sensitivity are needed, e.g., greater than 90% specificity and sensitivity with greater than 90% PPV. However, in a defined epidemic setting, e.g., a cruise ship infection, where PPV is high; the likelihood of false positives is low and likelihood of false negatives is high; and, when samples are submitted for confirmatory testing, it may prove desirable to have a lesser specificity such as 65% in order to yet further lower the number of false negative test results e.g. to a value less than about 5%.
J. Diagnostic and Therapeutic Kits
Kits are provided for carrying out the instant methods. The instant kit is distinguished from immunoassay kits by at least the presence of one or more of: (i) a PDZ domain polypeptide and (ii) printed instructions for conducting an assay to identify a high risk influenza A avian virus strain in a biological sample using the PDZ domain polypeptide. The kit allows for the identification of a viral protein in the patient sample rather than an antibody, making it more specific to an infected individual. The instant kit optionally contains one or more of the reagents, buffers or additive compositions or reagents disclosed supra; and, in certain embodiments the kit can also contain antibodies specific for influenza A viral PL, preferably NS1. In yet other embodiments, the instant kit can further comprise a means, such as a device or a system, for removing the influenza viral PL from other potential interfering substances in the biological sample. The instant kit can further include, if desired, one or more of various components useful in conducting an assay: e.g., one or more assay containers; one or more control or calibration reagents; one or more solid phase surfaces on which to conduct the assay; or, one or more buffers, additives or detection reagents or antibodies; one or more printed instructions, e.g. as package inserts and/or container labels, for indicating the quantities of the respective components that are to be used in performing the assay, as well as, guidelines for assessing the results of the assay. The instant kit can contain components useful for conducting a variety of different types of assay formats, including e.g. test strips, sandwich ELISA, Western blot assays, latex agglutination and the like. The subject reference, control and calibrators within the instant kits can contain e.g. one or more natural and non-natural influenza PL proteins, recombinant PL polypeptides, synthetic PL peptides, PDZ domain polypeptides, PDZ domain peptides and/or appropriate colorimetric and enzyme standards for assessing the performance and accuracy of the instant methods.
The instructions for practicing the subject methods are commonly recorded on a suitable recording medium and included with the kit, e.g., as a package insert. For example, the instructions can be printed on a substrate such as paper or plastic. In other embodiments, the instructions can be digitally recorded on an electronic computer-readable storage medium, e.g. CD-ROM, diskette and the like. In yet other embodiments, instructions for conducting the instant methods can be obtained by a user from a remote digital source, e.g. at an internet website in the form of a downloadable document file.
Optionally, the kits can include reagents for performing a general test for Influenza A as well as specific tests. For example a lateral flow test can have a lane for identifying the presence of a general influenza A virus and a lane for identifying whether that virus is Avian Influenza A. The general test can be any test that identified the presence of an influenza A virus, including the test for the presence of NS1. Other types of general influenza A that can be included can identify any Influenza A protein. Alternatively the presence of influenza A can be identified by the presence of antibodies in the blood of the patient. Finally, PCR tests can be used to generally identify the presence of influenza A.
K. Arrays
In yet other embodiments, the invention provides PDZ, antibody, and/or aptamer arrays consisting of different PDZ polypeptides, antibodies, and/or aptamers or comparable binding agents immobilized at identifiable selected locations on a solid phase. Each of the immobilized PDZ polypeptides, antibodies and/or aptamers in the array has a defined binding affinity and specificity for PL ligands, i.e., including identified binding interactions with PL in influenza viral proteins. The discriminatory activity of the array is contributed by (i) the binding affinity of the respective different PDZ polypeptides, antibodies, and/or aptamers; (ii) the binding specificities of the respective different PDZ polypeptides, antibodies, and/or aptamers for PL; and, (iii) the assay conditions, e.g., ionic strength, time, pH and the like. PDZ domains are highly specific, e.g., the PDZ domain in MAST205 is capable of distinguishing between C-terminal PL sequences containing TDV and SDV. Similarly, within the same PDZ protein the different respective domains can have different binding specificities and affinities, i.e., PSD-95 domains-1, -2 and -3 have different binding specificities and affinities. Applicants have cloned, expressed and disclosed in prior US patent applications, the sequences of more than 255 different human PDZ domains comprising greater than 90% of all the PDZ domains in the human genome. Mapped interactions of the PDZ domain fusion proteins with different PL peptides constitute the basis for selecting particular members of the instant influenza array. Unexpectedly, the selectivity of the array is based in the findings of: (i) distinguishingly different NS-1 PL amino acid sequence motifs in different strains of influenza A, as illustrated in the Examples section below; and, combined with (ii) the different PL sequence motifs in different influenza viral proteins, i.e., HA, NP, MA1, NS1 and the like.
Embodiments of the invention provide methods for distinguishing between the different strains of an Influenza A virus, or Influenza B, in a test sample based on the constituent binding properties of the PL in the influenza viral proteins, e.g., HA, NP, MA-1, NS1 and the like, in which the different strains and/or subtypes of influenza A and B produce a distinctive pattern of binding on the array. The methods involve the steps of: (a) bringing into contact aliquots of a test sample at different predefined positions in the array; (b) detecting the presence or absence of binding at a particular position in the array; (c) determining from the pattern of binding in the array that (i) influenza PL are present in test sample and (ii) that the pattern of PL binding in the array constitutes a distinguishing signature for a particular strain of influenza A or B virus. Representative examples of the influenza A viruses that are distinguishable based in arrays include e.g. H1N1, H2N2, H2N3, H2N5, H3N2, H3N8, H4N6, H5N1, H6N1, H6N2, H7N2, H7N3 and H7N7. Preferably, the array is at least partly based on the binding to NS1 PL. More preferably, the PDZ, antibody, and/or aptamer arrays specifically identify the presence of at least one NS1 PL, including: ESEV (SEQ ID NO:2), ESEI (SEQ ID NO:3), ESKV (SEQ ID NO:4), TSEV (SEQ ID NO:5), GSEV (SEQ ID NO:6), RSEV (SEQ ID NO:7), RSKV (SEQ ID NO:8), GSEI (SEQ ID NO:9), GSKV (SEQ ID NO:10), NICI (SEQ ID NO:11), TICI (SEQ ID NO:12), RICI (SEQ ID NO:13), DMAL (SEQ ID NO:14), DMTL (SEQ ID NO:15), DIAL (SEQ ID NO:16), DLDY (SEQ ID NO:17), SICL (SEQ ID NO:18), SEV, SEI, SKV and SKI. More preferably, the NS1 PL is ESEV (SEQ ID NO:2). Preferably, the PDZ protein is at least one of those selected from Tables 1 or 2, fragments or analogs. More preferably, the array includes at least one PDZ protein, antibody or aptamer mimic of any PDZ protein listed in Tables 1 and 2, analogs and active fragments. More preferably, the array includes a PDZ protein, antibody mimic and/or aptamer, including Outer membrane protein, PSD95 (PDZ #2); PSD95 (PDZ #1,2,3); DLG1 (PDZ #1); DLG1 (PDZ #1,2); DLG1 (PDZ #2); DLG2 (PDZ #1); DLG2 (PDZ #2); Magi3 (PDZ #1); PTN3 (PDZ #1); MAST2 (PDZ #1); NeDLG (PDZ #1,2); Shank1 d1; Shank2 d1; Shank3 d1; Syntrophin1 alpha; Syntrophin gamma 1; Magi1 (PDZ #1); Magi1 (PDZ #4); Tip1; PTPL1 (PDZ #1); Mint3 (PDZ #1); Lym Mystique (PDZ #1); DLG2 (PDZ #3); MUPP1 (PDZ #8); NeDLG (PDZ #1); DLG5 (PDZ #1); PSD95 (PDZ #1); NumBP (PDZ #3); LIMK1 (PDZ #1); KIAA0313; DLG1 (PDZ #2); Syntenin (PDZ #2); Pick1 or an analog or fragment and/or antibodies (or aptamers) that mimic any PDZ protein.
L. Lateral Flow Designs
Similar to a home pregnancy test, lateral flow devices work by applying fluid to a test strip that has been treated with specific biologicals. Carried by the liquid sample, phosphors labeled with corresponding biologicals flow through the strip and can be captured as they pass into specific zones. The amount of phosphor signal found on the strip is proportional to the amount of the target analyte.
A sample suspected of containing influenza A is added to a lateral flow device by some means, the sample is allowed to move by diffusion and a line or colored zone indicates the presence of Influenza A. The lateral flow typically contains a solid support (for example nitrocellulose membrane) that contains three specific areas: a sample addition area, a capture area containing one or more PDZ proteins and antibodies immobilized, and a read-out area that contains one or more zones, each zone containing one or more labels. The lateral flow can also include positive and negative controls. Thus, for example a lateral flow device in certain embodiments would perform as follows: an influenza PL protein is separated from other viral and cellular proteins in a biological sample by bringing an aliquot of the biological sample into contact with one end of a test strip, and then allowing the proteins to migrate on the test strip, e.g., by capillary action such as lateral flow. One or more PL binding agents such as PDZ polypeptide agents, antibodies, and/or aptamers are included as capture and/or detect reagents. Methods and devices for lateral flow separation, detection, and quantification are known in the art, e.g., U.S. Pat. Nos. 5,569,608; 6,297,020; and 6,403,383 incorporated herein by reference in their entirety. In one non-limiting example, a test strip comprises a proximal region for loading the sample (the sample-loading region) and a distal test region containing a PDZ polypeptide capture agent and buffer reagents and additives suitable for establishing binding interactions between the PDZ polypeptide and any influenza PL protein in the migrating biological sample. In alternative embodiments, the test strip comprises two test regions that contain different PDZ domain polypeptides, i.e., each capable of specifically interacting with a different influenza PL protein analyte.
The above screening processes can identify one or more types of compounds that can be incorporated into pharmaceutical compositions. These compounds include agents that are inhibitors of transcription, translation and post-translational processing of either at least one NS1 protein, at least one PDZ protein. The agents also may also inhibit or block binding of an NS1 and a PDZ protein, or mixtures thereof. These compounds also include agents that are inhibitors of either one or more NS1 proteins, one or more PDZ proteins or the interaction between an NS1 and a PDZ protein and have an inherent respiratory and/or digestive or epithelial cell-specific activity or imaging activity. The compounds also include conjugates in which a pharmaceutical agent or imaging component is linked to an inhibitor of either an NS1, a PDZ protein or the interaction between NS1 proteins and PDZ proteins. Conjugates comprising an agent with a pharmacological activity and a conjugate moiety having decreased substrate capacity for a PDZ protein relative to the agent alone are also provided for the purpose of reducing transport of the agent into non-infected cells, where the agent would confer undesired side effects. Preferably, the compound or agent inhibits or blocks the binding of at least one of the following PLs to a PDZ protein: ESEV (SEQ ID NO:2), ESEI (SEQ ID NO:3), ESKV (SEQ ID NO:4), TSEV (SEQ ID NO:5), GSEV (SEQ ID NO:6), RSEV (SEQ ID NO:7), RSKV (SEQ ID NO:8), GSEI (SEQ ID NO:9), GSKV (SEQ ID NO:10), NICI (SEQ ID NO:11), TICI (SEQ ID NO:12), RICI (SEQ ID NO:13), DMAL (SEQ ID NO:14), DMTL (SEQ ID NO:15), DIAL (SEQ ID NO:16), DLDY (SEQ ID NO:17), SICL (SEQ ID NO:18), SEV, SEI, SKV and SKI. More preferably, the NS1 PL that is blocked or inhibited is ESEV (SEQ ID NO:2). Preferably, the compound or agent inhibits the binding to at least one of the PDZ proteins from Tables 1 or 2. More preferably, the PDZ protein or interaction that is inhibited is at least one of: PSD95 (PDZ #2); PSD95 (PDZ #1,2,3); DLG1 (PDZ #1); DLG1 (PDZ #1,2); DLG1 (PDZ #2); DLG2 (PDZ #1); DLG2 (PDZ #2); Magi3 (PDZ #1); PTN3 (PDZ #1); MAST2 (PDZ #1); NeDLG (PDZ #1,2); Shank1 d1; Shank2 d1; Shank3 d1; Syntrophin1 alpha; Syntrophin gamma 1; Magi1 (PDZ #1); Magi1 (PDZ #4); Tip1; PTPL1 (PDZ #1); Mint3 (PDZ #1); Lym Mystique (PDZ #1); DLG2 (PDZ #3); MUPP1 (PDZ #8); NeDLG (PDZ #1); DLG5 (PDZ #1); PSD95 (PDZ #1); NumBP (PDZ #3); LIMK1 (PDZ #1); KIAA0313; DLG1 (PDZ #2); Syntenin (PDZ #2); Pick1 or an analog or fragment and/or antibodies (or aptamers) that mimic any PDZ protein.
One or more of the above entities can be combined with pharmaceutically-acceptable, non-toxic carriers or diluents, which are defined as vehicles commonly used to formulate pharmaceutical compositions for animal or human administration. The diluent is selected so as not to affect the biological activity of the combination. Examples of such diluents are distilled water, buffered water, physiological saline, phosphate buffered saline (PBS), Ringer's solution, dextrose solution, and Hank's solution. In addition, the pharmaceutical composition or formulation can also include other carriers, adjuvants, or non-toxic, nontherapeutic, nonimmunogenic stabilizers, excipients and the like. The compositions can also include additional substances to approximate physiological conditions, such as pH adjusting and buffering agents, toxicity adjusting agents, wetting agents, detergents and the like (see, e.g., Remington's Pharmaceutical Sciences, Mace Publishing Company, Philadelphia, Pa., 17th ed. (1985); for a brief review of methods for drug delivery, see, Langer, Science 249:1527-1533 (1990); each of these references is incorporated by reference in its entirety).
Pharmaceutical compositions for oral administration can be in the form of e.g., tablets, pills, powders, lozenges, sachets, cachets, elixirs, suspensions, emulsions, solutions, or syrups. Some examples of suitable excipients include lactose, dextrose, sucrose, sorbitol, mannitol, starches, gum acacia, calcium phosphate, alginates, tragacanth, gelatin, calcium silicate, microcrystalline cellulose, polyvinylpyrrolidone, cellulose, sterile water, syrup, and methylcellulose. Preserving agents such as methyl- and propylhydroxy-benzoates; sweetening agents; and flavoring agents can also be included. Depending on the formulation, compositions can provide quick, sustained or delayed release of the active ingredient after administration to the patient. Polymeric materials can be used for oral sustained release delivery (see “Medical Applications of Controlled Release,” Langer and Wise (eds.), CRC Pres., Boca Raton, Fla. (1974); “Controlled Drug Bioavailability,” Drug Product Design and Performance, Smolen and Ball (eds.), Wiley, New York (1984); Ranger and Peppas, 1983, J. Macromol. Sci. Rev. Macromol Chem. 23:61; see also Levy et al., 1985, Science 228: 190; During et al., 1989, Ann. Neurol. 25:351; Howard et al., 1989, J. Neurosurg. 71:105). Sustained release can be achieved by encapsulating conjugates within a capsule, or within slow-dissolving polymers. Preferred polymers include sodium carboxymethylcellulose, hydroxypropylcellulose, hydroxypropylmethylcellulose and hydroxyethylcellulose (most preferred, hydroxypropyl methylcellulose). Other preferred cellulose ethers have been described (Alderman, Int. J. Pharm. Tech. & Prod. Mfr., 1984, 5 (3) 1-9). Factors affecting drug release have been described in the art (Bamba et al., Int. J. Pharm., 1979, 2, 307). For administration by inhalation, the compounds for use according to the disclosures herein are conveniently delivered in the form of an aerosol spray preparation from pressurized packs or a nebulizer, with the use of a suitable propellant, e.g., dichlorodifluoromethane, trichlorofluoromethane, dichlorotetrafluoroethane, carbon dioxide or other suitable gas, or from propellant-free, dry-powder inhalers. In the case of a pressurized aerosol the dosage unit can be determined by providing a valve to deliver a metered amount. Capsules and cartridges of, e.g., gelatin for use in an inhaler or insufflator can be formulated containing a powder mix of the compound and a suitable powder base such as lactose or starch.
Effective dosage amounts and regimes (amount and frequency of administration) of the pharmaceutical compositions are readily determined according to any one of several well-established protocols. For example, animal studies (e.g., mice, rats) are commonly used to determine the maximal tolerable dose of the bioactive agent per kilogram of weight. In general, at least one of the animal species tested is mammalian. The results from the animal studies can be extrapolated to determine doses for use in other species, such as humans for example.
A compound can be administered to a patient for prophylactic and/or therapeutic treatments. A therapeutic amount is an amount sufficient to remedy a disease state or symptoms, or otherwise prevent, hinder, retard, or reverse the progression of disease or any other undesirable symptoms in any way whatsoever. In prophylactic applications, a compound is administered to a patient susceptible to or otherwise at risk of a particular disease or infection. Hence, a “prophylactically effective” amount is an amount sufficient to prevent, hinder or retard a disease state or its symptoms. In either instance, the precise amount of compound contained in the composition depends on the patient's state of health and weight.
An appropriate dosage of the pharmaceutical composition is determined, for example, using animal studies (e.g., mice, rats) are commonly used to determine the maximal tolerable dose of the bioactive agent per kilogram of weight. In general, at least one of the animal species tested is mammalian. The results from the animal studies can be extrapolated to determine doses for use in other species, such as humans for example.
The components of pharmaceutical compositions are preferably of high purity and are substantially free of potentially harmful contaminants (e.g., at least National Food (NF) grade, generally at least analytical grade, and more typically at least pharmaceutical grade).
To the extent that a given compound must be synthesized prior to use, the resulting product is typically substantially free of any potentially toxic agents, particularly any endotoxins, which may be present during the synthesis or purification process. Compositions are usually made under GMP conditions. Compositions for parenteral administration are usually sterile and substantially isotonic.
A. Antiviral Agents
Pharmaceutical compositions suitable for use in the present invention include compositions wherein the active agents are contained in an effective dosage. Anti-viral agents include inhibitors of NS1, PDZ, and/or NS1/PDZ interactions that preferably show at least 30, 50, 75, 95, or 99% inhibition of levels of NS1 or PDZ mRNA or protein. Protein expression can be quantified by forming immunological analyses using an antibody that specifically binds to the protein followed by detection of complex formed between the antibody and protein. mRNA levels can be quantified by, for example, dot blot analysis, in-situ hybridization, RT-PCR, quantitative reverse-transcription PCR (i.e., the so-called “TaqMan” methods), Northern blots and nucleic acid probe array methods. Preferably, the NS1 PL used to identify inhibitors is one of: ESEV (SEQ ID NO:2), ESEI (SEQ ID NO:3), ESKV (SEQ ID NO:4), TSEV (SEQ ID NO:5), GSEV (SEQ ID NO:6), RSEV (SEQ ID NO:7), RSKV (SEQ ID NO:8), GSEI (SEQ ID NO:9), GSKV (SEQ ID NO:10), NICI (SEQ ID NO:11), TICI (SEQ ID NO:12), RICI (SEQ ID NO:13), DMAL (SEQ ID NO:14), DMTL (SEQ ID NO:15), DIAL (SEQ ID NO:16), DLDY (SEQ ID NO:17), SICL (SEQ ID NO:18), SEV, SEI, SKV and SKI. More preferably, the NS1 PL used to identify inhibitors is ESEV (SEQ ID NO:2). Preferably, the PDZ protein used to identify inhibitors is at least one of those selected from Tables 1 or 2, fragments or analogs. More preferably, the PDZ protein used to identify inhibitors is at least one of: Outer membrane protein, PSD95 (PDZ #2); PSD95 (PDZ #1,2,3); DLG1 (PDZ #1); DLG1 (PDZ #1,2); DLG1 (PDZ #2); DLG2 (PDZ #1); DLG2 (PDZ #2); Magi3 (PDZ #1); PTN3 (PDZ #1); MAST2 (PDZ #1); NeDLG (PDZ #1,2); Shank1 d1; Shank2 d1; Shank3 d1; Syntrophin1 alpha; Syntrophin gamma 1; Magi1 (PDZ #1); Magi1 (PDZ #4); Tip1; PTPL1 (PDZ #1); Mint3 (PDZ #1); Lym Mystique (PDZ #1); DLG2 (PDZ #3); MUPP1 (PDZ #8); NeDLG (PDZ #1); DLG5 (PDZ #1); PSD95 (PDZ #1); NumBP (PDZ #3); LIMK1 (PDZ #1); KIAA0313; DLG1 (PDZ #2); Syntenin (PDZ #2); Pick1 or an analog or fragment and/or antibodies (or aptamers) that mimic any PDZ protein.
Anti-viral agents can include PL peptide therapeutics identified as binding to a PDZ protein that interacts with an influenza NS1 or other PL protein. Anti-viral agents include peptides including on based PL motifs or PDZ domains. Some exemplary peptides for inhibiting interactions between influenza virus PL and PDZ domains binding to the PLs are shown in table 11 (SEQ ID NOS:89-987). Other useful peptides are SEQ ID NOS: 2, 48, 53, 996 and 997, as described in the Examples. Therapeutic agents of the invention include the peptides themselves, truncations thereof including at least 5, 10, 15 or 20 contiguous residues starting at the C-terminus, and conservatively substituted variants and mimetics of all of these peptides, optionally incorporated into pharmaceutical compositions. Conservative substitutions, if any, preferably occur outside the C-terminal 3-4 amino acids of the peptides. Peptides that block binding of a pathogenic influenza PL to the PDZ are useful for treating pathogenic influenza. Preferably, the peptides shown in Table 11, truncations, conservatively substituted variants or mimetics thereof are linked to a transporter peptide (protein transduction domain) at the N-terminus of the peptide sequence. Several transporter peptide sequences can be used, including Tat and antennapedia (see also Example 7). Anti-viral therapeutics also include small molecules that inhibit the interaction between a viral PL and a PDZ, as well as Cox2 inhibitors (as identified in Table 8 herein). Some small molecule inhibitors have been identified in Tables 9 and 10 herein.
B. Methods of Screening for Anti-Viral Agents
Methods of screening for agents that bind to NS1 PL proteins and/or PDZ proteins are disclosed herein. The agents are initially screened for binding to the NS1 PL or the PDZ domain of the PDZ protein. Then they are tested for the ability to inhibit the PDZ/PL interaction. These methods are also provided below in “B. assay for anti-viral agents.” The binding assay can be performed in vitro using natural or synthetic PL proteins. Alternatively, natural or synthetic PDZ domain containing proteins can be used to identify agents capable of binding to a particular PDZ protein.
Methods of screening for anti-viral agents disclosed herein identify agents that block or inhibit the interaction between the viral PL and any PDZ protein that it interacts with. Inhibitors and DNA encoding them are screened for capacity to inhibit expression of NS1 and/or PDZ. An initial screen can be performed to select a subset of agents capable of inhibiting or stopping the PDZ/PL interaction. Such an assay can be performed in vitro using an isolated PDZ protein and PL protein or fragments thereof capable of binding to each other. Agents identified by such a screen can then be assayed functionally. Agents can also be screened in cells expressing PL proteins and either expressing the PDZ protein naturally or transformed to express the PDZ protein.
In addition to the diagnostic assays disclosed and illustrated above, embodiments provide assays for identifying candidate anti-viral agents capable of modulating one or more binding interactions occurring between an influenza viral PL and a host cell PDZ polypeptide in an influenza A infected cell. The instant methods involve testing the binding of a control PL, e.g., a synthetic PL peptide, to a PDZ domain polypeptide, e.g., a recombinant PDZ fusion protein, in the presence of an anti-viral test agent. A candidate anti-viral agent modulates the binding between the control PL and the PDZ domain polypeptide. Applicant has previously disclosed assays for measuring binding interactions between control PL and PDZ domain polypeptides in US and International patent applications, e.g., U.S. Pat. Nos. 5,569,608; 6,297,020; and 6,403,383 incorporated herein by reference in their entirety.
Particularly useful screening assays employ cells which express both one or more influenza NS1 PLs and one or more PDZ domain proteins. Such cells can be made recombinantly by co-transfection of the cells with polynucleotides encoding the proteins, or can be made by transfecting a cell which naturally contains one of the proteins with the second protein. In a particular embodiment, such cells are grown up in multi-well culture dishes and are exposed to varying concentrations of a test compound or compounds for a pre-determined period of time, which can be determined empirically. Whole cell lysates, cultured media or cell membranes are assayed for inhibition of the PL/PDZ interaction. Test compounds that significantly inhibit activity compared to control (as discussed below) are considered therapeutic candidates.
Isolated PDZ domain proteins or PL-binding fragments thereof, can be used for screening therapeutic compounds in any of a variety of drug screening techniques. Alternatively, isolated NS1 PL proteins or fragments containing the PL motif can be used The protein employed in such a test can be membrane-bound, free in solution, affixed to a solid support, borne on a cell surface, or located intracellularly. The formation of binding complexes between the PDZ domain or NS1 PL and the agent being tested can be measured. More specifically, a test compound is considered as an inhibitor of the PDZ/PL interaction if the interaction is significantly lower than the interaction measured in the absence of test compound. In this context, the term “significantly lower” means that in the presence of the test compound the PDZ/PL interaction, when compared to that measured in the absence of test compound, is measurably lower, within the confidence limits of the assay method.
Random libraries of peptides or other compounds can also be screened for suitability as inhibitors of the PDZ/PL binding, or for simply binding to either the PDZ domain protein or the NS1 PL protein. Combinatorial libraries can be produced for many types of compounds that can be synthesized in a step-by-step fashion. Such compounds include polypeptides, beta-turn mimetics, polysaccharides, phospholipids, hormones, prostaglandins, steroids, aromatic compounds, heterocyclic compounds, benzodiazepines, oligomeric N-substituted glycines and oligocarbamates. Large combinatorial libraries of the compounds can be constructed by the encoded synthetic libraries (ESL) method described in Affymax, WO 95/12608, Affymax, WO 93/06121, Columbia University, WO 94/08051, Pharmacopeia, WO 95/35503 and Scripps, WO 95/30642 (each of which is incorporated by reference for all purposes).
A preferred source of test compounds for use in screening for therapeutics or therapeutic leads is a phage display library. See, e.g., Devlin, WO 91/18980; Key, B. K., et al., eds., Phage Display of Peptides and Proteins, A Laboratory Manual, Academic Press, San Diego, Calif., 1996. Phage display is a powerful technology that allows one to use phage genetics to select and amplify peptides or proteins of desired characteristics from libraries containing 108-109 different sequences. Libraries can be designed for selected variegation of an amino acid sequence at desired positions, allowing bias of the library toward desired characteristics. Libraries are designed so that peptides are expressed fused to proteins that are displayed on the surface of the bacteriophage. The phage displaying peptides of the desired characteristics are selected and can be regrown for expansion. Since the peptides are amplified by propagation of the phage, the DNA from the selected phage can be readily sequenced facilitating rapid analyses of the selected peptides.
Phage encoding peptide inhibitors can be selected by selecting for phage that bind specifically to a PDZ domain protein and/or to an NS1 PL. Libraries are generated fused to proteins such as gene II that are expressed on the surface of the phage. The libraries can be composed of peptides of various lengths, linear or constrained by the inclusion of two Cys amino acids, fused to the phage protein or can also be fused to additional proteins as a scaffold. One can also design libraries biased toward the PL regions disclosed herein or biased toward peptide sequences obtained from the selection of binding phage from the initial libraries provide additional test inhibitor compound.
C. Types of Anti-Viral Agents
Any of the agents set out below can be used as pharmaceuticals as well as those identified in screening methods. Inhibitors can be identified from any type of library, including RNA expression libraries, bacteriophage expression libraries, small molecule libraries, peptide libraries. Inhibitors can also be produced using the known sequence of the nucleic acid and/or polypeptide. The compounds also include several categories of molecules known to regulate gene expression, such as zinc finger proteins, ribozymes, siRNAs and antisense RNAs.
(a) siRNA Inhibitors
siRNAs are relatively short, at least partly double stranded, RNA molecules that serve to inhibit expression of a complementary mRNA transcript. Although an understanding of mechanism is not required for practice of the invention, it is believed that siRNAs act by inducing degradation of a complementary mRNA transcript. Principles for design and use of siRNAs generally are described by WO 99/32619, Elbashir, EMBO J. 20, 6877-6888 (2001) and Nykanen et al., Cell 107, 309-321 (2001); WO 01/29058.
siRNAs of the invention are formed from two strands of at least partly complementary RNA, each strand preferably of 10-30, 15-25, or 17-23 or 19-21 nucleotides long. The strands can be perfectly complementary to each other throughout their length or can have single stranded 3′-overhangs at one or both ends of an otherwise double stranded molecule. Single stranded overhangs, if present, are usually of 1-6 bases with 1 or 2 bases being preferred. The antisense strand of an siRNA is selected to be substantially complementary (e.g., at least 80, 90, 95% and preferably 100%) complementary to a segment of a NS1 or PDZ transcript. Any mismatched based preferably occur at or near the ends of the strands of the siRNA. Mismatched bases at the ends can be deoxyribonucleotides. The sense strand of an siRNA shows an analogous relationship with the complement of the segment of the NS1 or PDZ transcript. siRNAs having two strands, each having 19 bases of perfect complementarity, and having two unmatched bases at the 3′ end of the sense strand and one at the 3′ end of the antisense strand are particularly suitable.
If an siRNA is to be administered as such, as distinct from the form of DNA encoding the siRNA, then the strands of an siRNA can contain one or more nucleotide analogs. The nucleotide analogs are located at positions at which inhibitor activity is not substantially effected, e.g. in a region at the 5′-end and/or the 3′-end, particularly single stranded overhang regions. Preferred nucleotide analogues are sugar- or backbone-modified ribonucleotides. Nucleobase-modified ribonucleotides, i.e. ribonucleotides, containing a non-naturally occurring nucleobase instead of a naturally occurring nucleobase such as uridines or cytidines modified at the 5-position, e.g. 5-(2-amino)propyl uridine, 5-bromo uridine; adenosines and guanosines modified at the 8 position, e.g. 8-bromo guanosine; deaza nucleotides, e.g. 7-deaza-adenosine; O- and N-alkylated nucleotides, e.g. N6-methyl adenosine are also suitable. In preferred sugar-modified ribonucleotides, the 2′ OH-group is replaced by a group selected from H, OR, R, halo, SH, SR, NH2, NHR, NR2 or CN, wherein R is C1-C6 alkyl, alkenyl or alkynyl and halo is F, CI, Br or I. In preferred backbone-modified ribonucleotides the phosphoester group connecting to adjacent ribonucleotides is replaced by a modified group, e.g. of phosphothioate group. A further preferred modification is to introduce a phosphate group on the 5′ hydroxide residue of an siRNA. Such a group can be introduced by treatment of an siRNA with ATP and T4 kinase. The phosphodiester linkages of natural RNA can also be modified to include at least one of a nitrogen or sulfur heteroatom. Modifications in RNA structure can be tailored to allow specific genetic inhibition while avoiding a general panic response in some organisms which is generated by dsRNA. Likewise, bases can be modified to block the activity of adenosine deaminase.
A number of segments within the NS1 or PDZ transcript are suitable targets for design of siRNAs. When a selected segment of NS1 PL is used to selectively target a subtype, the segment preferably shows a lack of perfect sequence identity with other NS1 PL regions of the transcript. Preferably, the selected segment of an NS1 or PDZ protein shows at least at least 1, 2, 3, 4 or more nucleotide differences from a corresponding segment (if any) of a NS1 PL. Target sites can be chosen from the coding region, 5′UTR and 3′UTR of NS1 or PDZ, in some cases, the PL site of NS1 is preferred. A preferred target site is that of the siRNA termed NS1 PL (see Examples). This site is at the C-terminus and is specific for subtypes of the Influenza A virus. Other preferred sites include the PL binding site of the PDZ protein.
siRNA can be synthesized recombinantly by inserting a segment of DNA encoding the siRNA between a pair of promoters that are oriented to drive transcription of the inserted segment in opposite orientations. Transcription from such promoters produces two complementary RNA strands that can subsequently anneal to form the desired dsRNA. Exemplary plasmids for use in such systems include the plasmid (PCR 4.0 TOPO) (available from Invitrogen). Another example is the vector pGEM-T (Promega, Madison, Wis.) in which the oppositely oriented promoters are T7 and SP6; the T3 promoter can also be used. Alternatively, DNA segments encoding the strands of the siRNA are inserted downstream of a single promoter. In this system, the sense and antisense strands of the siRNA are co-transcribed to generate a single RNA strand that is self-complementary and thus can form dsRNA. Vectors encoding siRNAs can be transcribed in vitro, or in cell culture or can be introduced into transgenic animals or patients for expression in situ. Suitable vectors are described below. The selection of promoters and optionally other regulatory sequences for recombinant expression can determine the tissue specificity of expression. For example, PDGF, prion, neural enolase, or thy-1 promoters are suitable for expression in the central nervous system.
The strands of an siRNAs can also be synthesized by organic chemical synthesis and annealed in vitro. If synthesized chemically or by in vitro enzymatic synthesis, the RNA can be purified prior to introduction into the cell. For example, RNA can be purified from a mixture by extraction with a solvent or resin precipitation, electrophoresis, chromatography; or a combination thereof. The RNA can be dried for storage or dissolve in an aqueous solution. The solution can contain buffers or salts to promote annealing, and/or stabilization of the duplex stands. siRNAs can be introduced into cells or organisms either as RNA or in the form of DNA encoding the RNA by a variety of approaches, as described below.
(b) Antisense Polynucleotides
Antisense polynucleotides can cause suppression by binding to, and interfering with, the translation of sense mRNA, interfering with transcription, interfering with processing or localization of RNA precursors, repressing transcription of mRNA or acting through some other mechanism. The particular mechanism by which the antisense molecule reduces expression is not critical.
Typically antisense polynucleotides comprise a single-stranded antisense sequence of at least 7 to 10 to typically 20 or more nucleotides that specifically hybridize to a sequence from mRNA of a gene. Some antisense polynucleotides are from about 10 to about 50 nucleotides in length or from about 14 to about 35 nucleotides in length. Some antisense polynucleotides are polynucleotides of less than about 100 nucleotides or less than about 200 nucleotides. In general, the antisense polynucleotide should be long enough to form a stable duplex but short enough, depending on the mode of delivery, to administer in vivo, if desired. The minimum length of a polynucleotide required for specific hybridization to a target sequence depends on several factors, such as G/C content, positioning of mismatched bases (if any), degree of uniqueness of the sequence as compared to the population of target polynucleotides, and chemical nature of the polynucleotide (e.g., methylphosphonate backbone, peptide nucleic acid, phosphorothioate), among other factors.
To ensure specific hybridization, the antisense sequence is at least substantially complementary to a segment of target mRNA or gene encoding the same. Some antisense sequences are exactly complementary to their intended target sequence. The antisense polynucleotides can also include, however, nucleotide substitutions, additions, deletions, transitions, transpositions, or modifications, or other nucleic acid sequences or non-nucleic acid moieties so long as specific binding to the relevant target sequence corresponding to RNA or its gene is retained as a functional property of the polynucleotide. Antisense polynucleotides intended to inhibit NS1 or PDZ protein expression are designed to show perfect or a substantial degree of sequence identity to a specific NS1 or PDZ gene or transcript and imperfect and a lower degree of sequence identity to different PDZ gene.
Some antisense sequences are complementary to relatively accessible sequences of mRNA (e.g., relatively devoid of secondary structure). This can be determined by analyzing predicted RNA secondary structures using, for example, the MFOLD program (Genetics Computer Group, Madison Wis.) and testing in vitro or in vivo as is known in the art. Another useful method for identifying effective antisense compositions uses combinatorial arrays of oligonucleotides (see, e.g., Milner et al., 1997, Nature Biotechnology 15:537).
Antisense nucleic acids (DNA, RNA, modified, analogues, and the like) can be made using any suitable method for producing a nucleic acid, such as the chemical synthesis and recombinant methods disclosed herein. Antisense RNA can be delivered as is or in the form of DNA encoding the antisense RNA. DNA encoding antisense RNA can be delivered as a component of a vector, or in nonreplicable form, such as described below.
(c) Zinc Finger Proteins
Zinc finger proteins can also be used to suppress expression of the NS1 or PDZ protein or nucleic acid or a specific NS1 subtype. Zinc finger proteins can be engineered or selected to bind to any desired target site within a target gene. In some methods, the target site is within a promoter or enhancer. In other methods, the target site is within the structural gene. In some methods, the zinc finger protein is linked to a transcriptional repressor, such as the KRAB repression domain from the human KOX-1 protein (Thiesen et al., New Biologist 2, 363-374 (1990); Margolin et al., Proc. Natl. Acad. Sci. USA 91, 4509-4513 (1994); Pengue et al., Nucl. Acids Res. 22:2908-2914 (1994); Witzgall et al., Proc. Natl. Acad. Sci. USA 91, 4514-4518 (1994). Methods for selecting target sites suitable for targeting by zinc finger proteins, and methods for design zinc finger proteins to bind to selected target sites are described in WO 00/00388. Methods for selecting zinc finger proteins to bind to a target using phage display are described by EP.95908614.1. The target site used for design of a zinc finger protein is typically of the order of 9-19 nucleotides. For inhibition of NS1 or PDZ protein or polynucleotide, a target site is chosen within the NS1 or PDZ protein or polynucleotide that shows imperfect or lack of substantial sequence identity to a different PDZ gene or transcript as discussed above. Methods for using zinc finger proteins to regulate endogenous genes are described in WO 00/00409. Zinc finger proteins can be administered either as proteins or in the form of nucleic acids encoding zinc fingers. In the latter situation, the nucleic acids can be delivered using vectors or in nonreplicable form as described below.
(d) Ribozymes
Ribozymes are RNA molecules that act as enzymes and can be engineered to cleave other RNA molecules at specific sites. The ribozyme itself is not consumed in this process, and can act catalytically to cleave multiple copies of mRNA target molecules. General rules for the design of ribozymes that cleave target RNA in trans are described in Haseloff & Gerlach, (1988) Nature 334:585-591 and Uhlenbeck, (1987) Nature 328:596-603 and U.S. Pat. No. 5,496,698.
Ribozymes typically include two flanking segments that show complementarity to and bind to two sites on a transcript (target subsites) and a catalytic region between the flanking segments. The flanking segments are typically 5-9 nucleotides long and optimally 6 to 8 nucleotides long. The catalytic region of the ribozyme is generally about 22 nucleotides in length. The mRNA target contains a consensus cleavage site between the target subsites having the general formula NUN, and preferably GUC. (Kashani-Sabet and Scanlon, (1995) Cancer Gene Therapy 2:213-223; Perriman, et al., (1992) Gene (Amst.) 113:157-163; Ruffner, et al., (1990) Biochemistry 29: 10695-10702); Birikh, et al., (1997) Eur. J. Biochem. 245:1-16; Perrealt, et al., (1991) Biochemistry 30:4020-4025).
The specificity of a ribozyme can be controlled by selection of the target subsites and thus the flanking segments of the ribozyme that are complementary to such subsites. For an inhibitor of NS1 or PDZ proteins, the target subsites are preferably chosen so that there are no exact corresponding subsites in other PDZ proteins and preferably no corresponding subsites with substantial sequence identity. Ribozymes can be delivered either as RNA molecules or in the form of DNA encoding the ribozyme as a component of a replicable vector or in nonreplicable form as described below.
(e). Antibodies
The compounds include antibodies, both intact and binding fragments thereof, such as Fabs, Fvs, which specifically bind to a protein encoded by a gene of the invention. Usually the antibody is a monoclonal antibody although polyclonal antibodies can also be expressed recombinantly (see, e.g., U.S. Pat. No. 6,555,310). Examples of antibodies that can be expressed include mouse antibodies, chimeric antibodies, humanized antibodies, veneered antibodies and human antibodies. Chimeric antibodies are antibodies whose light and heavy chain genes have been constructed, typically by genetic engineering, from immunoglobulin gene segments belonging to different species (see, e.g., Boyce et al., Annals of Oncology 14:520-535 (2003)). For example, the variable (V) segments of the genes from a mouse monoclonal antibody can be joined to human constant (C) segments. A typical chimeric antibody is thus a hybrid protein consisting of the V or antigen-binding domain from a mouse antibody and the C or effector domain from a human antibody. Humanized antibodies have variable region framework residues substantially from a human antibody (termed an acceptor antibody) and complementarity determining regions substantially from a mouse-antibody, (referred to as the donor immunoglobulin). See Queen et al., Proc. Natl. Acad. Sci. USA 86:10029-10033 (1989) and WO 90/07861, U.S. Pat. No. 5,693,762, U.S. Pat. No. 5,693,761, U.S. Pat. No. 5,585,089, U.S. Pat. No. 5,530,101 and Winter, U.S. Pat. No. 5,225,539. The constant region(s), if present, are also substantially or entirely from a human immunoglobulin. Antibodies can be obtained by conventional hybridoma approaches, phage display (see, e.g., Dower et al., WO 91/17271 and McCafferty et al., WO 92/01047), use of transgenic mice with human immune systems (Lonberg et al., WO93/12227 (1993)), among other sources. Nucleic acids encoding immunoglobulin chains can be obtained from hybridomas or cell lines producing antibodies, or based on immunoglobulin nucleic acid or amino acid sequences in the published literature.
(f). Mimetic Compounds
In particular embodiments, the subject candidate anti-viral compound identified in the instant screening methods compound is a peptidomimetic of the subject PDZ domain polypeptide or PL, i.e., a synthetic chemical compound that has substantially the same structural and/or functional characteristics as a subject PDZ domain or PL. The subject mimetic can be either entirely composed of synthetic, non-natural analogues of amino acids, or, is a chimeric molecule of partly natural peptide amino acids and partly non-natural analogs of amino acids. The mimetic can also incorporate any amount of natural amino acid conservative substitutions as long as such substitutions also do not substantially alter the mimetic's structure and/or inhibitory or binding activity. As with polypeptides of the invention which are conservative variants, routine experimentation determines whether a mimetic is within the scope of the invention, i.e., that its structure and/or function is not substantially altered. Thus, a mimetic composition is within the scope of the invention if it is capable of inhibiting binding between the subject polypeptides.
Mimetics can contain any combination of normatural structural components, which are typically from three structural groups: a) residue linkage groups other than the natural amide bond (“peptide bond”) linkages; b) non-natural residues in place of naturally occurring amino acid residues; or c) residues which induce secondary structural mimicry, i.e., to induce or stabilize a secondary structure, e.g., a beta turn, gamma turn, beta sheet, alpha helix conformation, and the like.
A polypeptide can be characterized as a mimetic when all or some of its residues are joined by chemical means other than natural peptide bonds. Individual peptidomimetic residues can be joined by peptide bonds, other chemical bonds or coupling means, such as, e.g., glutaraldehyde, N-hydroxysuccinimide esters, bifunctional maleimides, N,N=-dicyclohexylcarbodiimide (DCC) or N,N=-diisopropylcarbodiimide (DIC). Linking groups that can be an alternative to the traditional amide bond (“peptide bond”) linkages include, e.g., ketomethylene (e.g., —C(═O)—CH2— for —C(═O)—NH—), aminomethylene (CH2—NH), ethylene, olefin (CH═CH), ether (CH2—O), thioether (CH2—S), tetrazole (CN4—), thiazole, retroamide, thioamide, or ester (see, e.g., Spatola (1983) in Chemistry and Biochemistry of Amino Acids, Peptides and Proteins, Vol. 7, pp 267-357, A Peptide Backbone Modifications, Marcell Dekker, NY).
A polypeptide can also be characterized as a mimetic by containing all or some non-natural residues in place of naturally occurring amino acid residues. Normatural residues are well described in the scientific and patent literature; a few exemplary normatural compositions useful as mimetics of natural amino acid residues and guidelines are described below.
Mimetics of aromatic amino acids can be generated by replacing by, e.g., D- or L-naphylalanine; D- or L-phenylglycine; D- or L-2 thieneylalanine; D- or L-1, -2,3-, or 4-pyreneylalanine; D- or L-3 thieneylalanine; D- or L-(2-pyridinyl)-alanine; D- or L-(3-pyridinyl)-alanine; D- or L-(2-pyrazinyl)-alanine; D- or L-(4-isopropyl)-phenylglycine; D-(trifluoromethyl)-phenylglycine; D-(trifluoromethyl)-phenylalanine; D-p-fluorophenylalanine; D- or L-p-biphenylphenylalanine; K- or L-p-methoxybiphenylphenylalanine; D- or L-2-indole(alkyl)alanines; and, D- or L-alkylainines, where alkyl can be substituted or unsubstituted methyl, ethyl, propyl, hexyl, butyl, pentyl, isopropyl, iso-butyl, sec-isotyl, iso-pentyl, or a non-acidic amino acids. Aromatic rings of a normatural amino acid include, e.g., thiazolyl, thiophenyl, pyrazolyl, benzimidazolyl, naphthyl, furanyl, pyrrolyl, and pyridyl aromatic rings.
Mimetics of acidic amino acids can be generated by substitution by, e.g., non-carboxylate amino acids while maintaining a negative charge; (phosphono)alanine; sulfated threonine. Carboxyl side groups (e.g., aspartyl or glutamyl) can also be selectively modified by reaction with carbodiimides (R=—N—C—N—R═) such as, e.g., 1-cyclohexyl-3(2-morpholinyl-(4-ethyl) carbodiimide or 1-ethyl-3(4-azonia-4,4-dimetholpentyl) carbodiimide. Aspartyl or glutamyl can also be converted to asparaginyl and glutaminyl residues by reaction with ammonium ions.
Mimetics of basic amino acids can be generated by substitution with, e.g., (in addition to lysine and arginine) the amino acids ornithine, citrulline, or (guanidino)-acetic acid, or (guanidino)alkyl-acetic acid, where alkyl is defined above. Nitrile derivative (e.g., containing the CN-moiety in place of COOH) can be substituted for asparagine or glutamine. Asparaginyl and glutaminyl residues can be deaminated to the corresponding aspartyl or glutamyl residues.
Arginine residue mimetics can be generated by reacting arginyl with, e.g., one or more conventional reagents, including, e.g., phenylglyoxal, 2,3-butanedione, 1,2-cyclohexanedione, or ninhydrin, preferably under alkaline conditions.
Tyrosine residue mimetics can be generated by reacting tyrosyl with, e.g., aromatic diazonium compounds or tetranitromethane. N-acetylimidizol and tetranitromethane can be used to form O-acetyl tyrosyl species and 3-nitro derivatives, respectively.
Cysteine residue mimetics can be generated by reacting cysteinyl residues with, e.g., alpha-haloacetates such as 2-chloroacetic acid or chloroacetamide and corresponding amines, to give carboxymethyl or carboxyamidomethyl derivatives. Cysteine residue mimetics can also be generated by reacting cysteinyl residues with, e.g., bromo-trifluoroacetone, alpha-bromo-beta-(5-imidozoyl) propionic acid; chloroacetyl phosphate, N-alkylmaleimides, 3-nitro-2-pyridyl disulfide; methyl 2-pyridyl disulfide; p-chloromercuribenzoate; 2-chloromercuri-4 nitrophenol; or, chloro-7-nitrobenzo-oxa-1,3-diazole.
Lysine mimetics can be generated (and amino terminal residues can be altered) by reacting lysinyl with, e.g., succinic or other carboxylic acid anhydrides. Lysine and other alpha-amino-containing residue mimetics can also be generated by reaction with imidoesters, such as methyl picolinimidate, pyridoxal phosphate, pyridoxal, chloroborohydride, trinitrobenzenesulfonic acid, O-methylisourea, 2,4, pentanedione, and transamidase-catalyzed reactions with glyoxylate.
Mimetics of methionine can be generated by reaction with, e.g., methionine sulfoxide. Mimetics of proline include, e.g., pipecolic acid, thiazolidine carboxylic acid, 3- or 4-hydroxy proline, dehydroproline, 3- or 4-methylproline, or 3,3,-dimethylproline. Histidine residue mimetics can be generated by reacting histidyl with, e.g., diethylprocarbonate or para-bromophenacyl bromide.
Other mimetics include, e.g., those generated by hydroxylation of proline and lysine; phosphorylation of the hydroxyl groups of seryl or threonyl residues; methylation of the alpha-amino groups of lysine, arginine and histidine; acetylation of the N-terminal amine; methylation of main chain amide residues or substitution with N-methyl amino acids; or amidation of C-terminal carboxyl groups.
An amino acid of a subject polypeptide can also be replaced by an amino acid (or peptidomimetic residue) of the opposite chirality. Thus, any amino acid naturally occurring in the L-configuration (which can also be referred to as the R or S, depending upon the structure of the chemical entity) can be replaced with the amino acid of the same chemical structural type or a peptidomimetic, but of the opposite chirality, generally referred to as the D-amino acid, but which can additionally be referred to as the R- or S-form.
The mimetics of the invention can also include compositions that contain a structural mimetic residue, particularly a residue that induces or mimics secondary structures, such as a beta turn, beta sheet, alpha helix structures, gamma turns, and the like. For example, substitution of natural amino acid residues with D-amino acids; N-alpha-methyl amino acids; C-alpha-methyl amino acids; or dehydroamino acids within a peptide can induce or stabilize beta turns, gamma turns, beta sheets or alpha helix conformations. Beta turn mimetic structures have been described, e.g., by Nagai (1985) Tet. Lett. 26:647-650; Feigl (1986) J. Amer. Chem. Soc. 108:181-182; Kahn (1988) J. Amer. Chem. Soc. 110:1638-1639; Kemp (1988) Tet. Lett. 29:5057-5060; Kahn (1988) J. Molec. Recognition 1:75-79. Beta sheet mimetic structures have been described, e.g., by Smith (1992) J. Amer. Chem. Soc. 114:10672-10674. For example, a type VI beta turn induced by a cis amide surrogate, 1,5-disubstituted tetrazol, is described by Beusen (1995) Biopolymers 36:181-200. Incorporation of achiral omega-amino acid residues to generate polymethylene units as a substitution for amide bonds is described by Banerjee (1996) Biopolymers 39:769-777. Secondary structures of polypeptides can be analyzed by, e.g., high-field 1H NMR or 2D NMR spectroscopy, see, e.g., Higgins (1997) J. Pept. Res. 50:421-435. See also, Hruby (1997) Biopolymers 43:219-266, Balaji, et al., U.S. Pat. No. 5,612,895.
D. Improving Anti-Viral Agents
To improve acceptance and introduction of the anti-viral agent into a cell of choice, there are a number of known methods. For example, PEGylation of proteins can be used to make them more resistant to the immune system. Alternatively, intracellular signals or moieties can be added to proteins and vectors to allow them to more easily enter the cell of choice. Moieties that make the protein or vector specifically acceptable to uptake by infected cells can be added, in this case a ligand that is specific for a receptor expressed by respiratory cells. The moiety may be specific for an influenza receptor or cell-type specific receptor.
The instant therapeutic compounds may be further modified to make the compound more soluble or to facilitate its entry into a cell. For example, the compound may be PEGylated at any position, or the compound may be conjugated to a membrane translocating peptide such as a tat, Antennapedia or signal sequence membrane translocation peptide such as described by U. Langel, “Cell Penetrating Peptides”, CRC Press, Boca Rotan, 2002, i.e., incorporated herein by reference in its entirety.
A number of peptide sequences have been described in the art as capable of facilitating the entry of a peptide linked to these sequences into a cell through the plasma membrane (Derossi et al., 1998, Trends in Cell Biol. 8:84). For the purpose of this invention, such peptides are collectively referred to as “transmembrane transporter peptides”, which is used interchangeably with “cell penetrating peptides”. Examples of the latter cell penetrating peptides include, but are not limited to the following: namely, tat derived from HIV (Vives et al., 1997, J. Biol. Chem. 272:16010; Nagahara et al., 1998, Nat. Med. 4:1449), antennapedia from Drosophila (Derossi et al., 1994, J. Biol. Chem. 261:10444), VP22 from herpes simplex virus (Elliot and D'Hare, 1997, Cell 88:223-233), complementarity-determining regions (CDR) 2 and 3 of anti-DNA antibodies (Avrameas et al., 1998, Proc. Natl. Acad. Sci. U.S.A., 95:5601-5606), 70 KDa heat shock protein (Fujihara, 1999, EMBO J. 18:411-419) and transportan (Pooga et al., 1998, FASEB J. 12:67-77). In certain embodiments, a truncated HIV tat peptide may be employed.
E. Interferon Production
Interferon-α and -β (IFN-α/β) play key roles in innate cellular mechanisms of anti-viral resistance, e.g., inhibiting transcription and translation of viral sequences. Assembly of IFN-α/β receptor signaling complexes requires recruitment of factors including transcription factors, e.g. NF-κB, STAT and INF-induced transcription factor-3; and protein kinases to the receptor complex. It is believed RACK1 may serve as the scaffolding protein recruiting and/or binding PKC and STAT to the complex; possibly in association with Plectin, i.e., a hemidesmasome organizer. Recent data from other laboratories suggests that mumps and measles viruses may disrupt the INF-α/β signaling complex, i.e., the mumps V-protein reportedly associates with RACK1 and induces dissociation of STAT from the receptor complexes; and, in measles virus infected cells the viral C and/or V proteins reportedly inhibit phosphorylation of signaling kinases by associating with and “freezing” the INF-α/β receptor complex.
Interferon-α/β signaling inhibits pro-apoptotic responses promoting cell survival through nuclear mobilization of STAT and NFκB14. Interferon receptor signaling triggers activation of PKC-δ15 which, in turn, can down-regulate caspase 316, as well as, proinflammatory signaling through STAT17 and, in the airway, through NFκB18,19. PKC-δ activation also reportedly suppresses TNFα-induced apoptosis20,21. In this respect, avian influenza NS1 inhibition of IFN-α/β signaling seems destined to promote cell death and determine the severity of disease. Thus, candidate medicinal agents and novel molecular targets for drug development are those that interfere with and/or interrupt NS1 effects on IFN-α/β signaling. These agents promote a desired therapeutic effect of ameliorating one or more symptoms of disease in a subject infected with influenza A.
High-risk (see also pathogenic) avian strains of influenza A establish fulminant infections in humans, i.e., spreading rapidly beyond mucosal pulmonary tissues into circulation and the CNS. Without being bound to a particular theory, it is highly likely that certain of the latter effects result from inhibition of INF-α/β signaling mediated by non-structural influenza A viral proteins. Further, it is highly likely that viral proteins such as NS1 and NS2 inhibit intracellular PDZ domain-PL interactions requisite for effective IFN-α/β signaling and induction of cellular anti-viral resistance mechanisms.
Possible PDZ-ligand (PL) sequences were identified herein in INF-α/β receptor-1 (Accession No. 16166194), the C-terminal sequence “QDFV” (SEQ ID NO: 31), i.e., a possible class-1 PL sequence. Similarly, other potential members of the INF-α/β-receptor-1 signaling complex also contain putative C-terminal PL sequence motifs as follows: namely, MAP-1A (Accession No. 2119250) contains “KSRV” (SEQ ID NO: 32); MAP-1B (Accession No. 14165456/5174525) contains “KIEL” (SEQ ID NO: 33); MAP-1A/1B light chain-3 (Accession No. 12383056/18551443) contains C-terminal “KLSV” (SEQ ID NO: 34); Plectin-1 (Accession No. 4505877) contains C-terminal PL sequence motif “SAVA” (SEQ ID NO: 35); PKC-δ (Accession No. 509050) contains “KVLL” (SEQ ID NO: 36)); INF-inducible protein kinase (Accession No. 13637584) and INF-inducible elf2 alpha kinase (Accession No. 4506103) contain C-terminal sequence motifs “RHTC” (SEQ ID NO: 37); interferon alpha responsive transcription factor-3 (Accession No. 5174475) has C-terminal motif “LSLV” (SEQ ID NO: 38); and, interferon regulatory factor-2 (Accession No. 20141499/4504723) contains “VKSC” (SEQ ID NO: 39).
Thus, it is highly likely that PDZ domain-PL interactions play significant roles in viral pathogenesis and thus constitute targets for development of medicinal compounds.
Medicinal compounds capable of inhibiting the interaction of NS1 with the intracellular PDZ-domains of the IFN-α/β receptor complex include PL peptides, and mimetics thereof, peptide inhibitors of NS1 PL/IFN interactions, inhibitors of NS1 expression, cell permeable non-natural PDZ domain polypeptides, and mimetics thereof, and small molecule inhibitors capable of inhibiting the binding of NS1 PL to human the specific PDZ domains involved in the IFN-α/β response.
F. Methods of Treatment
Pharmaceutical compositions disclosed herein are used in methods of treatment of prophylaxis of Influenza A diseases.
As can be appreciated from the disclosure above, the present invention has a wide variety of applications. For example, the inhibitors of either NS1 protein, PDZ protein or the interaction between an NS1 and PDZ protein, can be used to identify an agent or conjugate that interacts with the transporter and that can cross into the infected cell. The inhibitors of either NS1 protein, PDZ protein or the interaction between NS1 protein and PDZ protein also can be used to increase the capacity of an agent to bind to an infected cell by identifying a conjugate moiety that binds to the infected cell and linking the conjugate moiety to the agent.
In prophylactic application, pharmaceutical compositions or medicants are administered to a patient susceptible to, or otherwise at risk for developing Influenza A infections in an amount sufficient to prevent, reduce, or arrest the development of influenza A infections. In therapeutic applications, compositions or medicants are administered to a patient suspected to develop, or already suffering from influenza in an amount sufficient to reverse, arrest, or at least partially arrest, the symptoms of influenza A infections. In both prophylactic and therapeutic regimes, active agents in the form of inhibitors of NS1, PDZ, and/or the NS1-PDZ interaction, of the present invention are usually administered in several dosages until a sufficient response has been achieved. However, in both prophylactic and therapeutic regimes, the active agents can be administered in a single dosages until a sufficient response has been achieved. Typically, the treatment is monitored and repeated dosages can be given. Furthermore, the treatment regimes can employ similar dosages; routes of administration and frequency of administration to those used in treating Influenza A infection or progression of an influenza A infection.
The amount of the inhibitors of NS1 protein, PDZ protein and/or the NS1/PDZ interaction and other active agents that can be combined with a carrier material to produce a single dosage form vary depending upon the disease treated, the mammalian species, and the particular mode of administration. The “effective dosage”, “pharmacologically acceptable dose” or “pharmacologically acceptable amount” for any particular patient can depend on a variety of factors including the activity of the specific compound employed, the species, age, body weight, general health, sex and diet of the patient being treated; the time and route of administration; the rate of metabolism or excretion; other drugs which are concurrently or have previously been administered; the type and severity of the disease; severity of side-effects, whether the patient is animal or human, and the like. Usually the patient is human, but nonhuman mammals, including transgenic mammals, can also be treated. Full length or active fragments of the active agents may be administered in effective dosages.
For any inhibitors of NS1 protein, PDZ protein and/or the NS1/PDZ interaction and other active agents used in the methods of the present invention, an effective dose for humans can be estimated initially from non-human animal models. An effective dose can be determined by a clinician using parameters known in the art. Generally, dosing begins with an amount somewhat less than the optimal effective dose. Dosing is then increased by small increments thereafter until an effective dosage is achieved. (See The Merck Manual of Diagnosis and Therapy, 16th Edition, §22, 1992, Berkow, Merck Research Laboratories, Rahway, N.J., which is incorporated herein by reference).
Dosages need to be titrated to optimize safety and efficacy. Toxicity and therapeutic efficacy of the compounds described herein can be determined by standard pharmaceutical procedures in experimental animals, e.g., by determining the LD50, (the dose lethal to 50% of the population tested) and the ED50 (the dose therapeutically effective in 50% of the population tested). The dose ratio between toxic and therapeutic effect is the therapeutic index and can be expressed as the ratio between LD50 and ED50. Compounds that exhibit high therapeutic indices are preferred. The data obtained from these nonhuman animal studies can be used in formulating a dosage range that is not toxic for use in humans. The dosage of such compounds lies preferably within a range of circulating concentrations that include the ED50 with little or no toxicity. The exact formulation, route of administration and dosage can be chosen by the individual physician in view of the patient's condition. (See, e.g., Fingl et al. (1975) In: The Pharmacological Basis of Therapeutics, Chapter 1, which is incorporated herein by reference).
G. Methods of Administration
Inhibitors of NS1 protein, PDZ protein and/or the NS1/PDZ interaction and other active agents can be delivered or administered to a mammal, e.g., a human patient or subject, alone, in the form of a pharmaceutically acceptable salt or hydrolyzable precursor thereof, or in the form of a pharmaceutical composition wherein the compound is mixed with suitable carriers or excipient(s) in an effective dosage. An effective regime means that a drug or combination of drugs is administered in sufficient amount and frequency and by an appropriate route to at least detectably prevent, delay, inhibit or reverse development of at least one symptom of influenza A infection. An “effective dosage”, “pharmacologically acceptable dose”, “pharmacologically acceptable amount” means that a sufficient amount of an inhibitors of NS1 proteins or expression, PDZ proteins or expression and/or the NS1/PDZ protein interaction, an active agent or inhibitors of NS1, PDZ protein and/or the NS1/PDZ protein interaction in combination with other active agents is present to achieve a desired result, e.g., preventing, delaying, inhibiting or reversing a symptom of influenza A infections or the progression of influenza A infections when administered in an appropriate regime.
Inhibitors of NS1 from influenza A, one or more PDZ proteins and/or the NS1/PDZ protein interaction and other active agents that are used in the methods of the present invention can be administered as pharmaceutical compositions comprising the inhibitors of NS1, PDZ protein and/or the NS1/PDZ protein interaction or active agent, together with a variety of other pharmaceutically acceptable components. Pharmaceutical compositions can be in the form of solids (such as powders, granules, dragees, tablets or pills), semi-solids (such as gels, slurries, or ointments), liquids, or gases (such as aerosols or inhalants).
Suitable formulations for use in the present invention are found in Remington's Pharmaceutical Sciences (Mack Publishing Company 1985) Philadelphia, Pa., 17th edition) and Langer, Science (1990) 249:1527-1533, which are incorporated herein by reference. The pharmaceutical compositions described herein can be manufactured in a conventional manner, i.e., mixing, dissolving, granulating, dragee-making, levigating, emulsifying, encapsulating, entrapping or lyophilizing processes.
Inhibitors of NS1, PDZ protein and/or the NS1/PDZ protein interaction and other active agents can be formulated with common excipients, diluents or carriers, and compressed into tablets, or formulated as elixirs or solutions for convenient oral administration. Inhibitors of NS1, PDZ protein and/or NS1/PDZ protein interaction and other active agents can also be formulated as sustained release dosage forms and the like.
Administration of the compounds can be achieved in various ways, including oral, buccal, rectal, parenteral, intraperitoneal, intradermal, transdermal, intratracheal, intravenous, and intramuscular administration. The compound can be administered in a local rather than systemic manner, in a depot or sustained release formulation. In addition, the compounds can be administered in a liposome. Moreover, the compound can be administered by gene therapy.
For buccal administration, the compositions can take the form of tablets or lozenges formulated in a conventional manner.
For administration by inhalation, the compounds for use according to the present invention are conveniently delivered in the form of an aerosol spray preparation from pressurized packs, a nebulizer or a syringe sprayer, with the use of a suitable propellant, e.g., dichlorodifluoromethane, trichlorofluoromethane, dichlorotetrafluoroethane, carbon dioxide or other suitable gas, or from propellant-free, dry-powder inhalers. In the case of a pressurized aerosol the dosage unit can be determined by providing a valve to deliver a metered amount. Capsules and cartridges of, e.g., gelatin for use in an inhaler or insufflator can be formulated containing a powder mix of the compound and a suitable powder base such as lactose or starch.
The compounds can be formulated for parenteral administration by injection, e.g., by bolus injection or continuous infusion. Formulations for injection can be presented in unit dosage form, e.g., in ampules or in multidose containers, with an added preservative. The compositions can take such forms as suspensions, solutions or emulsions in oil-based or aqueous vehicles, and can contain formulator agents such as suspending, stabilizing and/or dispersing agents. The compositions are formulated as sterile, substantially isotonic and in full compliance with all Good Manufacturing Practice (GMP) regulations of the U.S. Food and Drug Administration.
Inhibitors of NS1, PDZ protein and/or the NS1/PDZ protein interaction and other active agents can also be formulated in rectal compositions such as suppositories or retention enemas, e.g., containing conventional suppository bases such as cocoa butter, carbowaxes, polyethylene glycols or other glycerides, all of which melt at body temperature, yet are solidified at room temperature.
In addition to the formulations described previously, the compounds can also be formulated as a depot preparation. Such long acting formulations can be administered by implantation (e.g., subcutaneously or intramuscularly) or by intramuscular injection. Thus, for example, the compounds can be formulated with suitable polymeric or hydrophobic materials (e.g., as an emulsion in an acceptable oil) or ion exchange resins, or as sparingly soluble derivatives, for example, as a sparingly soluble salt. (See, e.g., Urquhart et al., (1984), Ann Rev. Pharmacol. Toxicol. 24:199; Lewis, ed., 1981, Controlled Release of Pesticides and Pharmaceuticals, Plenum Press, New York, N.Y., U.S. Pat. Nos. 3,773,919, and 3,270,960, which are incorporated herein by reference).
Alternatively, other delivery systems for hydrophobic pharmaceutical compounds can be employed. Liposomes and emulsions are well known examples of delivery vehicles or carriers for hydrophobic drugs. In some methods, long-circulating, i.e., stealth, liposomes can be employed. Such liposomes are generally described in Woodle, et al., U.S. Pat. No. 5,013,556, the teaching of which is hereby incorporated by reference. The compounds of the present invention can also be administered by controlled release means and/or delivery devices such as those described in U.S. Pat. Nos. 3,845,770; 3,916,899; 3,536,809; 3,598,123; and 4,008,719; the disclosures of which are hereby incorporated by reference.
For administration by gene therapy, genetic material (e.g., DNA or RNA) of interest is transferred into a host to treat or prevent Influenza A infection. In the present invention, the genetic material of interest encodes an inhibitor of NS1, PDZ and/or the NS1/PDZ interaction, an active agent or a fragment thereof. According to one aspect of the invention, the genetic material should be therapeutically effective. Many such proteins, vectors, DNA are known per se. (See Culver, K. W., “Gene Therapy”, 1994, p. xii, Mary Ann Liebert, Inc., Publishers, New York, N.Y., incorporated herein by reference in its entirety). For the purposes of example only, vectors can be selected from the group consisting of Moloney murine leukemia virus vectors, adenovirus vectors with tissue specific promoters, herpes simplex vectors, vaccinia vectors, artificial chromosomes, receptor mediated gene delivery, and mixtures of the above vectors. Gene therapy vectors are commercially available from different laboratories such as Chiron, Inc., Emeryville, Calif.; Genetic Therapy, Inc., Gaithersburg, Md.; Genzyme, Cambridge, Mass.; Somtax, Alameda, Calif.; Targeted Genetics, Seattle, Wash.; Viagene and Vical, San Diego, Calif.
Adenoviruses are promising gene therapy vectors for genetic material encoding inhibitors of NS1, PDZ and/or NS1/PDZ interaction, active agent or a fragment thereof. Adenovirus can be manipulated such that it encodes and expresses the desired gene product (e.g., inhibitors of NS1, PDZ and/or NS1/PDZ interaction or a fragment thereof) and at the same time is inactivated in terms of its ability to replicate in a normal lytic viral life cycle. Adenovirus expression is achieved without integration of the viral DNA into the host cell chromosome, thereby alleviating concerns about insertional mutagenesis. Furthermore, adenoviruses have been used as live enteric vaccines for many years with an excellent safety profile (Schwartz, A. R. et al. (1974) Am. Rev. Respir. Dis. 109:233-238). Finally, adenovirus mediated gene transfer has been demonstrated in a number of instances including transfer of alpha-1-antitrypsin and CFTR to the lungs of cotton rats (Rosenfeld, M. A. et al. (1991) Science 252:431-434; Rosenfeld et al., (1992) Cell 68:143-155). Furthermore, extensive studies to attempt to establish adenovirus as a causative agent in human cancer were uniformly negative (Green, M. et al. (1979) PNAS USA 76:6606).
The pharmaceutical compositions also can comprise suitable solid or gel phase carriers or excipients. Examples of such carriers or excipients include but are not limited to calcium carbonate, calcium phosphate, various sugars, starches, cellulose derivatives, gelatin, and polymers such as polyethylene glycols.
Examination of the influenza resource database of the NCBI revealed that NS1 protein sequences possess features consistent with the ability to bind to PDZ domains. Such sequences are designated PDZ domain ligand or PL. The PL motif in these Influenza NS1 proteins was identified to be S/T-X-V/I/L where the S is serine, T is threonine, V is valine, I is isoleucine, L is leucine and X is any amino acid. Of the 747 full-length human NS1 sequences in the NCBI database, 572 had this motif. Of the 345 full-length chicken NS1 sequences in the NCBI database, 237 had this motif. The data is summarized in Tables 3a-3e, and
Human PL fell into five sequence groups (see Table 3a): RSKI (SEQ ID NO:40), ESEV (SEQ ID NO:2), KSEV (SEQ ID NO: 41), RSEV (SEQ ID NO: 7), and RSKV (SEQ ID NO: 8). There was a strong association of subtypes with a particular PL motif; RSKI (SEQ ID NO: 40) with H3N2 (93%), ESEV (SEQ ID NO:2) with H5N1 (100%), KSEV (SEQ ID NO: 41) with H1N1 (100%, though numbers are small), RSEV (SEQ ID NO: 7) with H3N2 (98%), and RSKV (SEQ ID NO: 8) with H3N2 (95%).
Chicken PL fell into five sequence groups (see Table 3b): ESEI (SEQ ID NO:3), ESEV (SEQ ID NO:2), GSEV (SEQ ID NO: 6), ESKV (SEQ ID NO:4) and GSKV (SEQ ID NO: 10). There was a strong association of subtypes or groups of subtypes with a particular PL motif; ESEI (SEQ ID NO:3) with H7N2 (90%), ESEV (SEQ ID NO:2) with H5N1 (64%), ESKV (SEQ ID NO:4) with H5N2 (84%) and GSKV (SEQ ID NO: 10) with H5N2 (100%—but the numbers were somewhat small). If the notifiable avian influenza or H5 and H7 were combined, ESEI (SEQ ID NO:3) was 100% associated with NAI and ESEV (SEQ ID NO:2) was 83% associated with NAI.
Duck PL fell into three sequence groups (see Table 3c). Swine PL fell into seven sequence groups (see Table 3d). Equine PL fell into one sequence group (see Table 3e).
The non-random assortment of NS1 PL sequences with HN subtyping suggests a method of identifying HN subtypes by NS1 PL typing. PDZ binding profiles can be used to differentiate between the different PL sequences and act as the foundation for influenza subtyping.
Examination of three representative PL sequence groups, ESEV (SEQ ID NO:2), EPEV (SEQ ID NO: 27) and RSKV (SEQ ID NO: 8) revealed a possible origin of the PL. ESEV (SEQ ID NO:2) first appeared in avian isolates and did not enter into the human and mammalian host until 2003 (see
The above analysis demonstrated a new method for testing for the presence of influenza virus NS1 polypeptides using PL motifs specific to Influenza A and to specific subtypes. Identification of a specific PL is a means for identifying which strain of influenza virus is present in a sample.
This example describes the binding of PDZ proteins to various influenza A PL motifs. The PDZ proteins were assessed using a modified ELISA. Briefly, a GST-PDZ fusion was produced that contained the entire PDZ domain of the PDZ proteins. In addition, biotinylated peptides corresponding to the C-terminal 20 amino acids of various influenza A strain NS1 proteins were synthesized and purified by HPLC. Binding between these entities was detected through the “G” Assay, a colorimetric assay using avidin-HRP to bind the biotin and a peroxidase substrate. The sequences of the NS1 proteins from the specific influenza strains are shown as SEQ ID NOS: 42-47.
Binding of NS1 PL (or C terminus in the case of H5N1A) to human PDZ proteins was determined using both (i) biotinylated synthetic 20-mer peptides selected to mimic certain of the NS1 PL (or C terminus) sequences in the H5N1, H1N1, and H3N2 strains of Influenza A; and, (ii) recombinant NS1 proteins encoded by synthetic genes in recombinant systems, i.e., NS1 DNA synthesized and fused to sequences encoding a MBP immunochemical tag in an expression system (maltose binding protein; NEB; produced according to manufacturer's instructions).
Matrix graph Peptides and proteins were tested in an array format constituting a near complete set (255) of all the different PDZ domains in the human genome. Each PDZ domain polypeptide was expressed as a recombinant GST-PDZ polypeptide in a commercial glutathione S-transferase tagged expression system. Specific binding of biotinylated-PL peptides to PDZ domain polypeptides was detected using streptavidin-HRP and TMB substrate. Similarly, specific binding of NS1-MBP fusion proteins to PDZ domain polypeptides was visualized using biotinylated anti-MBP, streptavidin-HRP and TMB substrate. The relative strength of binding was analyzed and the strong and weak binders are shown for each PL. A PDZ protein that binds more strongly is preferable when used for capturing or identifying PL proteins. However for tests that use differential binding of the PDZ protein to various PL proteins, weak binding PDZ proteins may still be useful. The results were as follows: MBP.NS1H1N1 (RSEV; SEQ ID NO: 7) PL from strain A/Taiwan/1996 Ac.# AAC14269 (SEQ ID NO:42) was tested for binding to a variety of PDZ proteins. The following PDZ proteins were found to bind strongly: Rho-Gap 10, Syntrophin 1 alpha, outer Membrane, Magi2 d3, Magi1 d4, Tip43 d1, Magi1 d1, Tip 1, PSD95 d-1,2,3, PTPL1d2, PSD95 d2, INADL d8, DLG1d-1,2, Vartul d2, PSD95 d1, magi13 d1, DLG1d2, Mast2 d1, NeDLG d-1,2, SNPC 11a, DLG2 d2. The following PDZ proteins were found to bind weakly: Magi3 d2, PTN3 d1, DLG2 d1. In a titration study using a direct binding sandwich assay, PSD95 d-1,2,3 was found to bind with an EC50 of 1.29 μg/ml and Outer Membrane protein was found to bind with an EC50 of 1.25 μg/ml. Other measurement are shown in Table 4a.
MBP.NS1H3N2 (RSKV; SEQ ID NO: 8) PL (SEQ ID NO:43) from strain A/New York/31/2004 Ac.# AAX56415 (SEQ ID NO: 43) was tested for binding to a variety of PDZ proteins. The following PDZ proteins were found to bind strongly: Outer Membrane, PSD95 d-1,2,3, INADL d8, DLG1d-1,2, Grip 1d4, Shank 1, GoRasp1 d1, Sim GoRasp65, Syntenin d2, NeDLG d3, FLJ12615, KIAA0967, PTN3 d1, DLG2 d1, NeDLG1, d-1,2, DLG2 d2, mast1 d1, Kiaa1719d4, Kiaa1415 d1, and PICK1 FL. The following were found to bind weakly: Shank 2, NumbBP d3, psd95 d-1,2,3, and Mast2d1. In a titration study using a direct binding sandwich assay, PSD95 d-1,2,3 was found to bind with an EC50 of 25.3 μg/ml and INADL d8 was found to bind with an EC50 of 0.869 μg/ml. Other measurement are shown in Table 4b.
MBP.NS1H5N1A (EPEV; SEQ ID NO: 27) PL from strain A/Hong Kong/97/1998 Ac.# AAK49317 (SEQ ID NO:44) was tested for binding to a variety of PDZ proteins. The following PDZ proteins were found to bind strongly: ALP, PSD95 d1, and PICK FL. The following were found to bind weakly: INADL d8, NeDLG d-1,2, and KIAA1719 d4. In a titration study using a direct binding sandwich assay, Outer membrane protein was found to bind with an EC50 of 12.55 μg/ml and PSD95 d-1,2,3 was found to bind with an EC50 of 15.76 μg/ml. Other measurement are shown in Table 4c.
MBP.NS1H5N1B (ESEV; SEQ ID NO: 2) PL from strain A/Vietnam/1194/2004 Ac.# AAT73394 (SEQ ID NO:45) was tested for binding to a variety of PDZ proteins. The following PDZ proteins were found to bind strongly: DLG1d-1,2, LIM mystique d1, DLG2 d3, Vartul d2, PSD95 d1, Magi3 d1, DLG1d2, PTN-3 d1, DLG2 d1, NeDLG1d-1,2, Magi2 d5, DLG2 d2, and PSD95 d3 CS Bound, Magi2 d1, DLG1 d1, RhoGap10, Outer membrane, Magi1 d4, Tip 43, Tip1 d1, PSD95 d-1,2,3, Tip33 d1, PSD95 d2. The following were found to bind weakly: SIP1d2, Lim RiL, mint3 d2, ALP1, PSD95 d3, SEMCAP 3 d1, LIMK 1, Kiaa0613, Syntrophin Gamma 1, Magi2 d6, Mast2d1, Magi1 d5, INADL d3, Magi3 d2, syntrophin 1 Alpha, magi2 d3, par3L d2, Magi1 d1, Kiaa1719 d5, Vartul d1, and PTPL1 d1. In a titration study using a direct binding sandwich assay, PSD95 d1,2,3 was found to bind with an EC50 of 0.29 μg/ml and Outer Membrane protein was found to bind with an EC50 of 0.18 μg/ml. Other measurement are shown in Table 4d (ND means not done).
Peptide 1958 H5N1A (EPEV; SEQ ID NO: 27) PL from strain A/duck/ST/4003/2003 Ac.# AAF02349/6048830 (SEQ ID NO:46) was tested for binding to a variety of PDZ proteins. The following PDZ proteins were found to bind weakly: MAST2 d1, PSD95 d-1,2,3, and PSD95 d2. In a titration study using a direct binding sandwich assay, PSD95 d2 was found to bind with an EC50 of 3.8 μg/ml and PSD95 d-1,2,3 was found to bind with an EC50 of 4.1 μg/ml. Other measurement are shown in Table 4e.
Peptide 1959 H5N1B (ESEV; SEQ ID NO: 2) PL from strain A/chicken/Hong Kong/915/1997 Ac.# AAT73457/50296374 (SEQ ID NO:47) was tested for binding to a variety of PDZ proteins.
The PDZs that met specific criteria for hit classification are summarized in the Matrix Hits List tables 4a-e, showing the relative strength of the interaction. To be classified as a hit the OD of the NS1-PDZ interaction had to be greater than twice the average background, and it had to qualify as a hit in at least two samples. Hits classified as “weak” had an OD of less than 0.5, and hits classified as “strong” had an OD of greater than 0.5.
Peptide and fusion protein titrations were performed using the same detection system as described above for the Matrix assays. The Matrix Hits Lists were used to determine which PDZs would be titrated with NS1 to measure the strengths of the interactions. The results of the titrations are shown above with respect to each specific PL tested. The EC50s calculated for the titrated NS1-PDZ interactions are listed. The specific assays and methods that were used are provided below.
Peptides representing the C-terminal 20 amino acids of various Influenza A NS1 proteins, were synthesized by standard FMOC chemistry and biotinylated if not used as an unlabeled competitor. The peptides were purified by reverse phase high performance liquid chromatography (HPLC) using a Vydac 218TP C18 Reversed Phase column having the dimensions of 10*25 mm, 5 um. Approximately 40 mg of peptide was dissolved in 2.0 ml of aqueous solution of 49.9% acetonitrile and 0.1% Tri-Fluoro acetic acid (TFA). This solution was then injected into the HPLC machine through a 25 micron syringe filter (Millipore). Buffers used to get a good separation were (A) distilled water with 0.1% TFA and (B) 0.1% TFA with Acetonitrile. The separation occurred based on the nature of the peptides. A peptide of overall hydrophobic nature eluted off later than a peptide of a hydrophilic nature. Fractions containing the “pure” peptide were collected and checked by Mass Spectrometer (MS). Purified peptides were lyophilized for stability and later use.
1) Coat plate with 100 μl of 5 μg/ml goat anti GST, 0/N@ 4° C.
2) Dump coating antibodies out and tap dry
3) Blocking—Add 200 μl per well 2% BSA, 2 hrs at 4° C.
4) Prepare proteins in 2% BSA
Human nasal secretions were examined for the presence and amount of NS1 from Influenza A. Human nasal aspirates were collected and stored in M4 viral transport media (Remel, Inc, Lenexa, Kans.) at −80° C. Stored material was thawed and run on 10% SDS-PAGE. Western blot analysis was performed with monoclonal antibodies to NS1, 3H3 and 1A10 (Arbor Vita Corporation, Sunnyvale, Calif.). The results for six samples are shown in
To investigate the timeline of when NS1 was produced and secreted by cells infected with influenza A virus, MDCK cells were infected with human influenza A/PR/8 at a MOI of 0.1. Supernatant as well as cells were collected and lysed in 1% Triton X-100 and subjected to SDS-PAGE and western analysis with monoclonal antibody 3H3 which is pan-reactive to NS1. NS1 was detected in infected cells within 24 hours after infection and detected in the supernatant of infected cells within 48 hours (see
To verify that NS1 interacts with PDZ proteins in cells, a series of PDZ pull-down experiments were performed. 293 HEK cells were transfected with plasmids containing HA-NS1-H5N1B or with HA-NS1-H3N2. Lysates were prepared as described herein. Glutathione-sepharose-PDZ beads were prepared (10 ug of DLG1d1,2, 10 ug of NeDLGd1,2, and 10 ug PSD95d1,2,3) and used to pulldown 150 ug of lysate from transfected 293ET cells as shown in
Similarly, glutathione-sepharose-PDZ beads were prepared (40 ug of INADLd8) and used to pulldown 150 ug of lysate from 293ET cells transfected with H3N2. Following an overnight incubation at 4° C. and multiple washes with PBS, a western blot was prepared and probed a-HA (1:500) (Roche). INADL d8 successfully pulldown HA-H3N2 NS1 from cell lysate (
The conclusion is that the NS1 PL is functional within the cell and can interact with PDZ domains as determined by the MATRIX assay.
Monoclonal antibodies were prepared to specifically bind to subtype NS1 proteins, NS1 PL classes and for pan-specificity. The strategy for the generation of monoclonal antibodies to NS1 is as follows and the results are shown in Tables 5, 6, and 7:
Examples of lateral flow formats for detection of NS1 are provided in
The PDZ striped membrane was inserted into the NS1/anti-NS1 protein solution and flow initiated by capillary action and a wicking pad. NS1 was subtyped based on the pattern of PDZ reactivity; H1N1 binds to both PSD95 and INADL d8; H3N2 binds to INADL d8 only; H5N1 binds to PSD95 only. Influenza A subtyping was performed based on the results of the NS1 lateral flow using reactivity to PDZ and detection with a gold conjugated pan-reactive anti-NS1 monoclonal antibody.
In
A lateral flow assay to identify pathogenic Influenza A in a patient sample is produced having pan-specific antibodies deposited on the membrane. The patient sample is admixed with a mixture of gold-labeled antibodies that recognize all NS1 PL's. The sample is applied to the lateral flow test strip and if a pathogenic strain of influenza A is present a line is formed on the strip.
The strip tests were run using the following protocol and materials: The materials that were used included: strips previously striped with goat anti-mouse/PSD95 d1,2,3/INADL d8; TBST/2% BSA/0.25% Tween 20 buffer; Stocks of NS1 proteins MBP-H1N1, MBP-H3N2, MBP-H5N1A, and MBP-H5N1B “old” (Jon's) fast gold-conjugated F68-4B2 antibody; and Maxisorp ELISA plates. The procedure was performed as follows:
The test provided in
1. Prepare cards with a sample membrane and sink pad.
2. Stripe membrane with the PDZ protein and/or antibodies (see above for conc.)
3. Dry the membrane overnight at 56 degrees, then cut the cards into strips 4.26 mm wide.
4. Attach a glass fiber sample pad to the bottom of the strip and place the entire strip inside a cassette for testing.
5. Thaw sample to be tested and add 80 μl of sample to 20 μl of buffer. Pipette up and down several times to mix.
6. Spike 8 μl of the gold-conjugated (Au—) detector mix into the sample/buffer solution. This detector mix is 4 μl of Au—F68-4B2 with 4 μl of Au—F68-3D5. Pipette up and down several times to mix.
7. Add 100 μl of the prepared sample to the sample well on the cassette.
8. Read the test and control lines on the cassette at 15 minutes post-addition of sample. The control line is clearly visible for any test results to be read reliably. Flu A positive samples are noted with (+). Flu A negative samples are noted with (−). The top arrow is pointing to the control and the bottom arrow is pointing to the test. In both cases the top line is a control line (goat anti-mouse mAb), the second line down is the test line (mixture of F64-3H3 and F68-4H9 mAbs for the Pan-Flu A Test and PSD95 d-1,2,3 for the Avian test). 2 ng of H5N1 protein was tested for the Avian test. The bottom circular spot is the sample well. In
c shows three of twenty human samples that were tested with the format shown in
In
In this example, compounds were selected for analysis as inhibitors of PDZ/PDZ ligand interactions. The following 23 drugs were screened against select PDZ/PL pairs (numbers 1-17 are COX inhibitors). 1. Niflumic acid, 2. Ibuprofen, 3. Naproxen sodium, 4. Diclofenac sodium salt, 5. Acetylsalicylic acid, 6. Salicylic acid, 7. Flurbiprofen, 8. Sulindac sulphide, 9. Sulindac, 10. Etodolac, 11. Indomethacin, 12. Ketorolac Tris salt, 13. Ketoprofen, 14. Mefenamic acid, 15. Carprofen, 16. Baclofen, 17. Fenoprofen, 18. Benztropine mesylate, 19. Amitriptyline HCl, 20. Cromolyn sodium, 21. Desipramine HCl, 22. Clomipramine HCl, and 23. Nortriptyline HCl. In the description below, Section A provides the experiments that were performed using COX inhibitors, Section B provides the experiments that were performed using small molecule inhibitors and Section C provides the experiments that were performed using peptide inhibitors. Table 8 provides the PDZ/PL interactions that were used to identify inhibitors in sections A-C. The PL sequences used were SEQ ID NOS:54-59. The results are shown in Table 11-13.
A. COX inhibitors were selected based on two criteria: 1. The presence of a carboxylate group which may interact favorably at the position zero of the PDZ, and 2. a hydrophobic or aromatic group near the carboxylate which may be placed at the position zero of the PDZ. The hydrophobic or aromatic group was not absolutely necessary but was preferred.
COX molecules were subject to screening in a matrix/array competition assay format at 250 uM drug concentration, i.e., assays where docking of ligands to solid phase PDZ domain in fusion proteins was assessed in the presence and absence of the small molecule competitor as described previously. The results are as follows. MAGI1 d1/AVC1857 was inhibited by Sulindac sulphide. The PSD95 d1/AVC1912 interaction was inhibited by Fenoprofen. The PSD95 d2/AVCAA345 interaction was not significantly inhibited by any of the drugs in the assay. The PSD95 d2/AVCAA348 interaction was inhibited by Fenoprofen. The PSD95 d3/AVC1916 interaction was inhibited by Fenoprofen. The SHANK1/AVC1965 interaction was inhibited by Fenoprofen. The TIP1/AVCAA56 interaction was inhibited by Sulindac sulphide. The other drugs did not show significant inhibition in this assay. The two main small molecule hits were Sulindac Sulphide and Fenoprofen.
The results show that COX inhibitors can be used as inhibitors of PDZ/PDZ ligand interactions and derivatives of these can be useful therapeutics for PDZ based targets and that of those tested, Sulindac Sulphide and Fenoprofen showed the strongest inhibition.
B. Small Molecule Inhibitors of PDZ/PDZ ligand interactions were predicted from molecular modeling. In silico screening with Accelrys software (Accelrys, San Diego, Calif.) was used to model and dock a 650,000 molecule library (ChemDiv, San Diego, Calif.; Blanca Pharmaceuticals, Mountain View, Calif.) with 4 different PDZ domain mimics. The molecular modeling was based on finding compounds that had the capability of interacting with the PDZ via electrostatic, hydrogen bonding and hydrophobic interactions.
The best hits from in silico screening were subject to screening in a matrix/array competition assay format, i.e., assays where docking of ligands to solid phase PDZ domain in fusion proteins was assessed in the presence and absence of the small molecule competitor as described elsewhere. The small molecules were screened for inhibition of the PDZ/PDZ ligand interactions listed in Table 9. The chemical structures and formulas of the small molecule inhibitors tested can be found with reference to any public database of small molecules known to one of skill in the art. Other examples of small molecule inhibitors can be found in United States Provisional application ARBV:002USP1, entitled “Small Molecule Inhibitors of PDZ Interactions,” filed ______, herein incorporated by reference in its entirety. The small molecule concentration used in the screen was ˜250 uM. The results of these screens are shown in Table 10.
With reference to Tables 10 and 11 which summarize the results, the small molecules were considered as hits based on the OD(450) readout of the assay either as weak, medium or strong: Weak hit: >40% reduction in OD relative to control, Medium Hit: ˜40-60% reduction in OD relative to control, Strong Hit: >40% reduction in OD.
The best of the hits in this latter analysis were then subject to titration binding studies, i.e., titration of small molecule in the same competition assay to estimate and IC50 value and the results are summarized Table 10.
Based on in silico screening, various small molecule inhibitors of PDZ/PL interactions were identified. These molecules can be used to block PDZ/PL interactions of therapeutic value, including Influenza A NS1/PDZ interactions.
C. Peptide therapeutic inhibitors were identified and tested (see Table 11). Each influenza A NS1 protein type containing a PL (H5N1, H3N2 and H1N1) has the potential to interact with several PDZs. Each of these PDZ's may in itself be a potential therapeutic target against the relevant Influenza A strain, and as such, blocking the PDZ with a peptide may have therapeutic utility. In order to identify potential therapeutic peptides, an AVC proprietary database was searched for PDZ ligands of each of the PDZ's. The AVC database contained PL/PDZ interactions that were identified as such based on a proprietary ELISA based assay (G assay) previously described. The criterion used to identify promising PL's was based on the following three criteria: 1) OD(450 nm)>=0.5, 2) Relative standard deviation of measurements<0.25, 3) Peptide concentration=<20 micromolar.
Based on a database of PDZ/PL binding interactions, structure and binding data, C-terminal sequences were identified as most likely to bind to the PSD95 d2 structure (SEQ ID NO:1, for example) based on PSD95 d2 structure and binding data. Thus, preferred peptide therapeutic inhibitors for the Avian FluA (H5N1) are based on peptides that bind to PSD95 d2 and, the optimum and preferred peptide sequences that bind to PSD95 d2 conform to the consensus sequence: E/D/N/Q-S/T-D/E/Q/N-V/L (SEQ ID NO:48)
Using this consensus sequence, the following are examples of preferred C-terminal sequences for peptide inhibitors that bind to the PSD95 d2 domain:
Potential PDZ ligand therapeutic peptides for each PDZ are summarized in Table 11. Table 11 sets out the PL peptide identifier (AVC ID) in the first column, the PL peptide name (derived from the protein from which it was derived) in the second column, the peptide sequence in the third column and the sequence identification number in the last column. Each part of the Table contains a heading identifying the PDZ protein that the PLs will bind. The peptides shown in table 11 or truncations thereof that leave the C-terminal PL are agents suitable for treating influenza. The PL peptide therapeutics that block binding of a pathogenic influenza PL to the PDZ are useful for treating pathogenic influenza. For example, the C-terminal sequences (3 to 20 amino acids long) of each of these peptides (SEQ ID NOS:89-987) is converted into a therapeutic by attaching a transporter peptide (protein transduction domain) to the N-terminus of the peptide sequence. Subfragments of these peptides of at least 5 amino acids long with the C-terminal 3 amino acids conserved are used as therapeutic inhibitors of the viral PL/PDZ interaction for each PDZ listed in Table 11, preferably, at least 6 amino acids long, 7 amino acids long, 8 amino acids long, 9 amino acids long, and 10 amino acids long. Preferably at least the C-terminal 4 amino acids are conserved, more preferably, the C-terminal 5 amino acids are conserved, the C-terminal 6 amino acids, or the C-terminal 7 amino acids. The peptide therapeutics also include peptides containing conservative substitutions of the amino acids in the peptide mimetics. However, preferably the conservative substitution is in a region other than the last 3 or 4 amino acids. Several transporter peptide sequences are used, including Tat and antennapedia. The peptides are subjected to further analysis by identifying those peptides that inhibit PDZ/PL interactions using the A or G PDZ assays as described in Example 2. Those peptides that are shown to be inhibitory are subjected to further studies in vitro and in an animal model of influenza.
In previous sections, the NS1 PL motif, ESEV (SEQ ID NO:2), was associated with the highly virulent/lethal phenotype seen in avian subtypes such as H5N1. Since the PL portion of NS1 overlaps with NS2, the impact of avian PL conservation on NS2 sequence in the overlap region were analyzed. NS1 and NS2 use different reading frames over the overlapping region and this places constraints on the choice of codons that can be used. The analysis identified that the sequence variation in this region changes the protein sequence of NS1 but not NS2 (see Tables 12 and 13—In Table 12 STYPE refers to the Subtype of the virus). Specifically, in H5N1 the PL sequences ESEV (SEQ ID NO:2), EPEV (SEQ ID NO:27) and ESKV (SEQ ID NO:4) did not change the protein sequence in NS2, maintaining a serine (S or Ser) at position 70 of NS2. In contrast, benign subtypes such as H3N2 contained nucleotide sequences that led to a glycine at position 70. The only exception to this was the 1918 strain H1N1, responsible for the lethal pandemic of 1918, expressed the PL, KSEV (SEQ ID NO:41), that resulted in a serine at position 70 like the H5N1 strain. The NS1 PL sequences shown in Table 12 are ESEV (SEQ ID NO:2), EPEV (SEQ ID NO:27), ESKV (SEQ ID NO:4), RSKV (SEQ ID NO:8), KSEV (SEQ ID NO:41), and RSEV (SEQ ID NO:7), the SEQ ID NOs for the NS1 C-Terminal coding region are identified in the Table as are the SEQ ID NOs for the NS2 REGION (See Tables 12 and 13). Therefore, a serine at position 70 in the Influenza A virus NS2 protein correlates with the virulence of the virus. As a result, the serine at position 70 can be used as a marker for high virulence while a glycine at position 70 in NS2 can be used as a marker for a more benign clinical course. The variation at position 70 of NS2 is used as a diagnostic marker and a therapeutic target below. The serine substitution permits this sequence to be phosphorylated and possibly regulated by kinases.
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A mucous sample is taken from a patient that presents with symptoms of influenza A. The sample is treated to be more fluid for use in a lateral flow format. A lateral flow format is produced using the protocol presented in Example 6, except that a nucleic acid that is complementary to the sequence comprising the overlap and containing the serine 70 in the NS2 protein from Table 12 is used to identify capture agents to capture any NS2 containing a serine at position 70 present in the sample. The capture agent includes complementary nucleic acids for all known virulent influenza A strains. A positive result indicates that the patient should be treated for a highly virulent form of Influenza A virus.
A monoclonal antibody based test is identical except that a series of antibodies that specifically recognize the NS2 overlap regions including the serine 70 from Table 12 are used as capture agents.
A therapeutic agent that blocks the interaction between the NS2 and a target is used. Specifically, the therapeutic agents block the binding at the serine 70 position of the NS2 protein. Peptides or small molecules therapeutic agents are administered to a patient that has been infected with Influenza A or prior to infection in an amount sufficient to block the interaction between NS2 and its target. The administration is via inhalation and the treatment is continued until the patient is free of symptoms and/or the patient is no longer in danger of contracting the disease.
All publications and patents cited in this specification are herein incorporated by reference as if each individual publication or patent were specifically and individually indicated to be incorporated by reference. Genbank records referenced by GID or accession number, particularly any polypeptide sequence, polynucleotide sequences or annotation thereof, are incorporated by reference herein. The citation of any publication is for its disclosure prior to the filing date and should not be construed as an admission that the present invention is not entitled to antedate such publication by virtue of prior invention.
While the present invention has been described with reference to the specific embodiments thereof, it should be understood by those skilled in the art that various changes may be made and equivalents may be substituted without departing from the true spirit and scope of the invention. In addition, many modifications may be made to adapt a particular situation, material, composition of matter, process, process step or steps, to the objective, spirit and scope of the present invention. All such modifications are intended to be within the scope of the claims appended hereto. All publications and patent applications mentioned in this specification are herein incorporated by reference to the same extent as if each individual publication or patent application was specifically and individually indicated to be incorporated by reference.
This application is a continuation of U.S. application Ser. No. 11/481,411; filed Jul. 3, 2006, which claims the benefit of U.S. Provisional Applications Nos. 60/696,221 filed Jul. 1, 2005; 60/726,377 filed Oct. 12, 2005; 60/765,292 filed Feb. 2, 2006; and 60/792,274 filed Apr. 14, 2006, each of which is herein incorporated by reference in its entirety.
| Number | Date | Country | |
|---|---|---|---|
| 60792274 | Apr 2006 | US | |
| 60765292 | Feb 2006 | US | |
| 60726377 | Oct 2005 | US | |
| 60696221 | Jul 2005 | US |
| Number | Date | Country | |
|---|---|---|---|
| Parent | 11481411 | Jul 2006 | US |
| Child | 12539535 | US |
