BRIEF DESCRIPTION OF DRAWINGS
FIG. 1 shows an embryo/endosperm structure derived from a 1-day post-pollinated microinjected nucellar slab after 21 days in vitro culture. The nucellar slab, isolated from a transgenic plant stably transformed with PHP10006 (Ubi::FRT1::GFP::35S::MOPAT::FRT1::GUS::FRT5), was injected with Agrobacterium containing PHP17797 (RB::Ubi-FRT1::FLPm::35S::Bar::FRT5::LB). The FLP enzyme expressed from PHP17797 mediated excision of the DNA between the FRT1 sites in PHP10006 in the injected slab, thereby creating an operable linkage of the promoterless GUS gene to the Ubi promoter, resulting in GUS expression. The blue spot (arrow) shows the GUS expression in the embryo area, demonstrating T-DNA transfer and gene expression from the injected Agrobacterium. Bar=1 mm.