Claims
- 1. A method of promoting angiogenesis comprising contacting a localized area of tissue with a PEG polymer in an amount effective to induce angiogenesis within the area of tissue.
- 2. The method of claim 1, wherein the PEG polymer further comprises a matrix.
- 3. The method of claim 2, wherein the matrix is selected from the group consisting of alginate, alginate/poly-L-lysine/alginate, and agarose/poly-L-lysine/alginate.
- 4. The method of claim 2, wherein the matrix comprises heparin sepharose beads.
- 5. The method of claim 3, wherein the matrix is in the form of a capsule which is surrounded by the PEG polymer.
- 6. The method of claim 1, further comprising contacting an angiogenic protein, or an expression vector encoding an angiogenic protein, with the area of tissue.
- 7. The method of claim 4, wherein the heparin sepharose bead contains an angiogenic protein or an expression vector encoding an angiogenic protein.
- 8. The method of claim 5, wherein the matrix core contains an angiogenic protein or an expression vector encoding an angiogenic protein.
- 9. The method of any one of claims 6, 7 or 8, wherein the angiogenic protein is selected from the group consisting of M-CSF, GM-CSF, VEGF-A, VEGF-B, VEGF-C, VEGF-D, basic FGF, PDGF-B, Angiopoietin 1, Angiopoietin 2, erythropoietin, BMP-2, BMP-4, BMP-7, TGF-beta, IGF-1, Osteopontin, Pleiotropin, Activin, and Endothelin-1.
- 10. The method of any one of claims 6, 7 or 8, wherein the expression vector is selected from the group consisting of adenoviral vectors, retroviral vectors, RNA vectors, DNA vectors, naked DNA, liposomes, cationic lipids, lentiviral vectors, AAV, and transposons.
- 11. The method of claim 1, wherein the PEG polymer is contacted with the tissue area by injection.
RELATED INFORMATION
[0001] This application claims priority to U.S. Provisional Patent Application Serial No. 60/205,346, filed on May 18, 2000, and to U.S. Provisional Patent Application Serial No. 60/211,323, filed on Jun. 14, 2000, both of which are incorporated by reference herein in their entirety. The contents of all patents, patent applications, and references cited throughout this specification also are hereby incorporated by reference in their entireties.
Provisional Applications (2)
|
Number |
Date |
Country |
|
60205346 |
May 2000 |
US |
|
60211323 |
Jun 2000 |
US |