Claims
- 1. A polymer matrix incorporating catalase co-immobilized with an analytic enzyme which generates hydrogen peroxide, wherein the concentration of catalase ranges from about 100 units/ml to about 1000 units/ml.
- 2. The polymer matrix of claim 1, which is pH-sensitive.
- 3. The polymer matrix of claim 1, which has a crosslinking proportion of between about 0.5 mol % and about 6 mol %.
- 4. The polymer matrix of claim 1, which when hydrated has a thickness ranging from about 0.1 mm to about 3.0 mm.
- 5. The polymer matrix of claim 1, wherein the analytic enzyme is glucose oxidase.
- 6. The polymer matrix of claim 1, wherein the matrix is composed of hydroxypropyl methacrylate, N,N-dimethylaminoethyl methacrylate, and tetraethyleneglycol dimethacrylate.
- 7. A biosensor or analyte-responsive drug delivery device which contains a polymer matrix and an analytic enzyme that generates hydrogen peroxide, wherein the analytic enzyme is co-immobilized in the biosensor or drug delivery device with catalase at a concentration ranging from about 100 units/ml to about 900 units/ml.
- 8. The biosensor or drug delivery device of claim 7, wherein the matrix is pH-sensitive.
- 9. The biosensor or drug delivery device of claim 7, wherein the matrix is not pH-sensitive.
- 10. The biosensor or drug delivery device of claim 7, which has a crosslinking proportion of between about 0.5 mol % and about 6 mol %.
- 11. The biosensor or drug delivery device of claim 7, which when hydrated has a thickness ranging from about 0.1 mm to about 3.0 mm.
- 12. The biosensor or drug delivery device of claim 7, wherein the analytic enzyme is glucose oxidase.
- 13. The biosensor or drug delivery device of claim 7, wherein the analyte is detected by means of a pressure sensor.
- 14. The biosensor or drug delivery device of claim 7, wherein the analyte is detected by amperometric means.
- 15. A method of making a polymer matrix for use in a biosensor or analyte-responsive drug delivery device containing an analytic enzyme that generates hydrogen peroxide, including a step of co-immobilizing the analytic enzyme with catalase at a concentration ranging from about 100 units/ml to about 1000 units/ml.
- 16. The method of claim 16, wherein the polymer matrix is formulated to have a crosslinking proportion of between about 0.5 mol % and about 6 mol %.
- 17. The method of claim 16, wherein the polymer matrix is formed to have a thickness when hydrated of between about 0.1 mm and about 3.0 mm.
- 18. The method of claim 16, wherein the analytic enzyme is glucose oxidase.
- 19. A method of making a biosensor or analyte-responsive drug delivery device, comprising the steps of:
providing an analyte-sensitive enzyme that generates hydrogen peroxide, providing catalase, providing a pre-gel solution of polymerizable constituents, combining the catalase at a concentration of between about 100 units/ml and about 1000 units/ml with the analytic enzyme in the pre-gel solution to produce an analytic pre-gel solution, placing the analytic pre-gel solution in an appropriate support structure, subjecting the analytic pre-gel solution to appropriate conditions to form a shaped polymer matrix having the catalase and the analytic enzyme co-immobilized therein, providing sensing components capable of detecting a change proportional to the activity of the analyte-sensitive enzyme, providing structural components necessary to construct a biosensor, and functionally associating the shaped polymer matrix with the sensing and structural components to produce a biosensor.
- 20. The method of claim 19, wherein the polymerizable constituents are hydroxypropyl methacrylate, N,N-dimethylaminoethyl methacrylate, and tetraethyleneglycol dimethacrylate, the shaped polymer matrix has a thickness of between about 0.1 mm and about 0.4 mm, and the analyte-sensitive enzyme is glucose oxidase.
Parent Case Info
[0001] This application for a utility patent is a continuation in part of a previously filed patent PCT/US98/20750, now U.S. patent application Ser. No. 09/308,392 and claims the benefit of U.S. Provisional Patent Application No. 60/194,042, filed Apr. 3, 2000.
Government Interests
[0002] This patent was supported by US government NIH grant (R43 DK55958-01) and the government may have certain rights herein.
Provisional Applications (1)
|
Number |
Date |
Country |
|
60194042 |
Apr 2000 |
US |
Continuation in Parts (1)
|
Number |
Date |
Country |
Parent |
09308392 |
May 1999 |
US |
Child |
09824552 |
Apr 2001 |
US |