Claims
- 1. Copolymers comprising a boronate containing polymer that adsorbs to a biological tissue and a polymer that does not adsorb to biological tissue.
- 2. The copolymer of claim 1, wherein the tissue adsorbing polymer comprises a boronate group selected from the group consisting of phenylboronic acid (PBA), 2-carboxyethaneboronic acid, 1,2-dicarboxyethaneboronic acid, β, β′-dicarboxyethaneboronate, β, γ-dicarboxypropaneboronate, 2-nitro- and 4-nitro-3-succinamidobenzene boronic acids, 3-nitro-4-(6-aminohexylamido)phenyl boronic acid, {4-[(hexamethylenetetramine)methyl]phenyl} boronic acid, 4-(N-methyl)carboxamidobenzene boronic acid, 2-{[(4-boronphenyl)methyl]-ethylammonio} ethyl and 2-{[(4-boronphenyl)methyl]diethylammonio} -ethyl groups, succinyl-3-aminophenylboronic acid, 6-aminocaproyl-3-aminophenylboronic acid, 3-(N-succinimidoxycarbonyl)aminophenylboronate, p-(ω-aminoethyl)phenylboronate, p-vinylbenzeneboronate, N-(3-dihydroxyborylphenyl)succinamic acid, N-(4-nitro-3-dihydroxyborylphenyl)succinamic acid, O-dimethylaminomethylbenzeneboronic acid, 4-carboxybenzeneboronic acid, 4-(N-octyl)carboxamidobenzeneboronic acid, 3-nitro-4-carboxybenzeneboronic acid, 2-nitro-4-carboxybenzeneboronic acid, 4-bromophenylboronate, p-vinylbenzene boronate, 4-(ω-aminoethyl)phenylboronate, catechol [2-(diethylamino)carbonyl, 4-bromomethyl]phenyl boronate, and 5-vinyl-2-dimethylaminomethylbenzeneboronic acid.
- 3. The copolymer of claim 1, wherein the tissue adsorbing polymer comprises phenylboronic acid.
- 4. The copolymer of claim 1, wherein the non tissue binding polymer is selected from the group consisting of polyalkylene oxides, mixed polyalkylene oxides having a solubility of at least one gram/liter in aqueous solutions, water soluble polysaccharides, polyvinyl alcohol, poly-N-vinyl pyrrolidone, noncationic polyacrylates, noncationic polymethacrylates, and mixtures and copolymers thereof.
- 5. The copolymer of claim 1, wherein the non tissue binding polymer is polyethylene glycol.
- 6. The copolymer of claim 1, wherein the copolymer is a block copolymer.
- 7. The copolymer of claim 1, wherein the copolymer is a brush copolymer, having a backbone of the boronate containing tissue adsorbing polymer and bristles of the non tissue binding polymer.
- 8. The copolymer of claim 1, wherein the copolymer is a dendritic copolymer.
- 9. The copolymer of claim 1 wherein the copolymer is in a composition further comprising a pharmaceutically acceptable carrier.
- 10. The copolymer of claim 1 further comprising a biodegradable linkage.
- 11. The copolymer of claim 1 further comprising a bioactive, prophylactic, or diagnostic agent.
- 12. The copolymer of claim 11 wherein the agent is chemically coupled to the polymer.
- 13. The copolymer of claim 1 wherein the boronate containing polymer that adsorbs to a biological tissue comprises electron withdrawing groups so that the effective pKa of the copolymer is lower than that of the copolymer without electron withdrawing groups.
- 14. The copolymer of claim 1 wherein the boronate containing polymer is flanked by amine groups so that the effective pKa of the copolymer is lower than that of the copolymer without amine groups.
- 15. A method for altering cell or tissue interaction comprising applying an effective amount of a copolymer comprising a boronate containing polymer that adsorbs to the cell or tissue and a polymer that does not adsorb to the cell or tissue, as defined by any of claims 1-14, to alter the cell or tissue interaction.
- 16. The method of claim 15, wherein the copolymer is applied in an amount effective to minimize or prevent tissue adhesion.
- 17. The method of claim 15, wherein the copolymer is applied in an amount effective to decrease the risk of thrombosis, decrease inflammation, or decrease the abnormal proliferation of cells.
- 18. The method of claim 15, wherein the copolymer is applied in an amount effective to enhance wound healing.
- 19. The method of claim 15, wherein the copolymer is applied in an amount effective for the delivery of a bioactive, prophylactic, or diagnostic agent.
- 20. A method for altering cell or tissue interaction with a surface comprising applying an effective amount of a copolymer comprising a boronate containing polymer that adsorbs to the surface and a polymer that does not adsorb to the cell or tissue, to the surface to alter the cell or tissue interaction with the surface.
- 21. The method of claim 20, wherein the copolymer is applied to coat a prosthesis or other implantable device.
- 22. The method of claim 20, wherein the copolymer is applied to a cell or tissue.
- 23. The method of claim 15, wherein the site where adhesion is to be prevented is a region where tissue has been injured.
- 24. The method of claim 15, wherein the copolymer is applied at the time of or shortly after tissue injury.
- 25. The method of claim 15, wherein the copolymer is applied using a catheter or other minimally invasive technique.
CROSS REFERENCE TO RELATED APPLICATION
[0001] This application claims priority to provisional patent application Serial No. 60/056,854 filed on Aug. 27, 1997 by Jeffrey A. Hubbell, Natalie D. Winblade, and Donald L. Elbert and entitled “Steric Protection of Biological Surfaces by the Adsorption of Copolymers Containing Poly(ethylene glycol) and Phenylboronic Acid”.
STATEMENT REGARDING FEDERALLY FUNDED RESEARCH
[0002] The U.S. Government has certain right in this invention by virtue of a National Science Foundation Graduate Fellowship to Natalie Winblade and NIH Grant number HL56297 to Jeffrey Hubbell.
Provisional Applications (1)
|
Number |
Date |
Country |
|
60056854 |
Aug 1997 |
US |
Divisions (1)
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Number |
Date |
Country |
Parent |
09140781 |
Aug 1998 |
US |
Child |
10403344 |
Mar 2003 |
US |