Patent Application
20140370520
The present application relates to methods of diagnosing cancers, particularly bladder and prostate cancer, using compounds which are useful in the detection of NQO1 or NQO2 expressing cells.
Bladder cancer is the ninth most common cancer worldwide. It is more prevalent in men than women. Worldwide an estimated 356,600 new cases of bladder cancer occur each year (2008), with approximately 20,000 deaths per year. The highest bladder cancer incidence rates are generally found in industrially developed countries, particularly in North America and Western Europe.
In developed countries approximately 90% of bladder cancers are transitional cell carcinomas (TCC, bladder warts) while the remaining 10% are squamous cell carcinomas and adenocarcinomas. Superficial transitional cell carcinoma tends to spread only within the bladder unless it is left untreated for a long period of time. TCC may spread along the lining of the bladder but does not penetrate deeply into the bladder (unless left untreated) and the cells are shed into the urine.
Superficial bladder tumours can be managed very effectively by repeated resection. The tumour is removed (resected) via a cystoscope which is passed up the urethra. This type of treatment is highly invasive. Superficial bladder tumours tend to recur intermittently and may require resection on a repeated basis. Invasive bladder cancer requires a more aggressive approach. In the early stages, the tumours can be resected surgically either by partial or complete removal of the bladder. This may require major surgery which will require the creation of an ileal conduit. Routine ‘check cystoscopy’ is used to detect the recurrence of tumours particularly in the early stages and further treatment initiated if required. The time interval between check cystoscopies is usually 3-4 months initially but may be increased if the bladder remains free of tumour at subsequent investigation. Check cystoscopy is recommended for a period of several years to ensure that the tumour has not returned. Approximately 85% of patients with bladder cancer suffer recurrence within 5 years, the majority of patients within 2 years. The high recurrence rate may, to a large extent, be attributed to the tumours in the bladder being present in multiple locations which may be missed on examination or are too small to be seen by the surgeon during initial resection. Hence there is a clinical need for a sensitive, specific and non-invasive means of bladder cancer detection.
The need for a diagnostic test to detect early stage bladder cancer (superficial transitional cell carcinoma) is warranted due to the high risk of recurrence (85%) following surgical or chemotherapeutic intervention in the first 5 years. In addition to methods requiring invasive examination and sampling, several non-invasive tests have been described. A recent review (Shariat et al, 2008) of such methods concluded that none of the tests reviewed met all of the criteria of an ideal tumour marker. Thus there is a need for a simple, rapid, accurate and non-invasive method for early detection of bladder cancer and the invention disclosed herein provides a solution to this problem.
Elevated levels of NAD(P)H:quinone reductase-1 (NQO1, E.C. 1.6.99.2) and other related redox enzymes in superficial bladder cancer have been detected when compared to invasive transitional cell carcinoma (Li et al, 2001, J. Urol., 166, 2500-2505; Choudry et al., 2001, Br. J. Cancer, 85, 1137-1146). This significant difference has been exploited to treat early stage bladder cancers by using NQO1-specific agents. Benzoquinone-drug conjugate systems have been developed to specifically target NQO1-rich tumour cells.
NQO1 is also over-expressed in the cells of various other types of cancer, including breast, non-small cell lung, pancreas, colon and prostate cancers, relative to normal cells.
The related enzyme NQO2 is also over-expressed in cancer cells, including those set out above.
The present invention takes advantage of the over-expression of NQO1 and NQO2 in cancer cells relative to normal cells and relates to enzyme substrates that are activated by NQO1 or NQO2, resulting in a detectable signal in the presence of cancer cells. By contrast, minimal signal is observed in the absence of cancer cells (and thus greatly reduced amounts of NQO1 and/or NQO2).
In a first aspect of the invention there is provided a method of determining the presence or absence, in a biological sample from a patient, of cancer cells which over-express NQO1 and/or NQO2, the method comprising:
i. contacting the biological sample, or a processed derivative thereof, with a compound of general formula (I)
wherein R1, R2, R3, R4 and R5 each independently represent hydrogen, halogen, NR6R7, C(O)NR6R7 or C1-C6 alkyl, —O(C1-C6 alkyl) or C(O)O(C1-C6 alkyl), any of which may optionally be substituted with one or more reactive substituents;
R6 and R7 each independently represent hydrogen or C1-C6 alkyl optionally substituted with halo; or
R1 and R2 together with the carbon atoms to which they are attached form a 5- or 6-membered optionally substituted aromatic, heteroaromatic, carbocyclic or heterocyclic ring system;
z-XH;
or an ion of the formula:
z-X−;
wherein z and X are as defined in general formula (I), wherein presence of the compound or ion indicates the presence in the sample of cancer cells which over-express NQO1 and/or NQO2.
In a second aspect of the invention there is provided a method of determining the presence or absence, in a biological sample from a patient, of cancer cells which over-express NQO1 and/or NQO2, the method comprising:
i. contacting the biological sample, or a processed derivative thereof, with a compound of general formula (Ia), (Ib), (Ic), (Id), (Ie) or (If) or a salt of any thereof; wherein the biological sample contains or is suspected of containing cancer cells which over-express NQO1 and/or NQO2;
ii. optionally, in the case where the cancer cells over-express or are suspected of over-expressing NQO2, adding an NQO2 co-substrate to the sample; and
iii. determining the presence or absence of a compound of the formula:
z-XH;
or an ion of the formula:
z-X−;
wherein z and X are as defined in general formula (I), wherein presence of the compound or ion indicates the presence in the sample of cancer cells which over-express NQO1 and/or NQO2;
wherein:
general formula (Ia) is:
wherein R1, R2, R3, R4, R5, X, Y and z are as defined for general formula (I);
R1′, R2′, R3′, R4′ and R5′ each independently represent hydrogen, halogen, NR6R7, C(O)NR6R7 or C1-C6 alkyl, —O(C1-C6 alkyl) or C(O)O(C1-C6 alkyl), any of which may optionally be substituted with one or more reactive substituents;
R6 and R7 each independently represent hydrogen or C1-C6 alkyl optionally substituted with halo; or
R1′ and R2′ together with the carbon atoms to which they are attached form a 5- or 6-membered optionally substituted aromatic, heteroaromatic, carbocyclic or heterocyclic ring system;
wherein R1, R2, R4, X and z are as defined for general formula (I) and Rx is H, or C1-C3 alkyl;
general formula (Ic) is:
wherein R4, R5, X, Y and z are as defined for general formula (I); and
R10, R11, R12 and R13 each independently represent hydrogen, halogen, NR6R7, C(O)NR6R7 or C1-C6 alkyl, —O(C1-C6 alkyl) or C(O)O(C1-C6 alkyl), any of which may optionally be substituted with one or more reactive substituents;
R6 and R7 each independently represent hydrogen or C1-C6 alkyl optionally substituted with halo;
general formula (Id) is:
wherein X and z are as defined for general formula (I); and
R10, R11, R12 and R13 each independently represent hydrogen, halogen, NR6R7, C(O)NR6R7 or C1-C6 alkyl, —O(C1-C6 alkyl) or C(O)O(C1-C6 alkyl), any of which may optionally be substituted with one or more reactive substituents;
R6 and R7 each independently represent hydrogen or C1-C6 alkyl optionally substituted with halo;
general formula (Ie) is:
wherein X, Y and z are as defined for general formula (I); and
R10, R11, R12 and R13 each independently represent hydrogen, halogen, NR6R7, C(O)NR6R7 or C1-C6 alkyl, —O(C1-C6 alkyl) or C(O)O(C1-C6 alkyl), any of which may optionally be substituted with one or more reactive substituents;
R6 and R7 each independently represent hydrogen or C1-C6 alkyl optionally substituted with halo;
general formula (If) is:
wherein z is as defined for general formula (I);
R10, R11, R12 and R13 each independently represent hydrogen, halogen, NR6R7, C(O)NR6R7 or C1-C6 alkyl, —O(C1-C6 alkyl) or C(O)O(C1-C6 alkyl), any of which may optionally be substituted with one or more reactive substituents;
R6 and R7 each independently represent hydrogen or C1-C6 alkyl optionally substituted with halo;
R14 is H or C1-C6 alkyl.
They compounds of general formulae (I), (Ia), (Ib), (Ic), (Id), (Ie) and (If) are susceptible to reduction to yield a product of formula z-XH or an anion of formula z-X− and a reduced residue. As will be discussed in greater detail below, the moiety z is a detectable marker and the remainder of the molecule is chosen such that the detectable signal emitted by the group z is modified when it forms part of the compound of general formula (I), (Ia), (Ib), (Ic), (Id), (Ie) or (If) compared with the signal emitted by the compound z-XH or the ion z-X−.
Quinone or benzoquinone compounds of formula (I) and (Ia), indoles of formula (Ib), nitro-based compounds of general formula (Ic), (Id) and (Ie) and compounds of general formula (If) are all known in the art. For example, compounds of general formula (Ia) are taught by Huang et al, Org. Letters, 8(2), 2665-268 (2006) and compounds of general formulae (I), (Ib), (Ic), (Id) and (Ie) are discussed in Blanche et al, Tetrahedron, 65(25), 4892-4903 (2009).
The nature of the detectable moiety, i.e. whether it is a compound of the formula z-XH or an anion of formula z-X− will depend upon the nature of the detectable marker z, the detection method used and the environment in which the detection method is conducted. Therefore, hereafter, references to a compound of formula z-XH should be taken also to comprehend an anion of formula z-X−. Thus, methods for the detection and/or quantification of a compound z-XH also include methods for the detection and/or quantification of an anion z-X−.
Further, reference to a processed derivative of a biological sample includes reference to a biological sample after it has been treated, typically for the purpose of preparing it for the method of the invention or preserving it prior to undertaking the said method and involves the use of conventional techniques well known to those skilled in the art of taking, preparing or preserving biological samples.
In the present specification “C1-C6 alkyl” refers to a straight or branched saturated hydrocarbon chain having one to six carbon atoms. Examples include methyl, ethyl, n-propyl, isopropyl, t-butyl and n-hexyl.
The term “C1-C3 alkyl” refers to an alkyl group having from 1 to 3 carbon atoms.
The term “aromatic” in the context of the present specification refers to a ring system with aromatic character having 5 or 6 ring carbon atoms. Aromatic groups may optionally be substituted with one or more substituents independently selected from halo, methyl, ethyl, methoxy, ethoxy, nitro and cyano. Phenyl is a particularly suitable aryl group.
The term “heteroaromatic” in the context of the specification refers to a ring system with aromatic character having 5 or 6 ring atoms, at least one of which is a heteroatom selected from N, O and S. Examples of heteroaromatic groups include pyridine, pyrimidine, furan, thiophene, oxazole, diazole and triazole. Heteroaromatic groups may optionally be substituted with one or more substituents independently selected from halo, methyl, ethyl, methoxy, ethoxy, nitro and cyano.
The term “carbocyclic” in the context of the present specification refers to a non-aromatic ring system having 5 or 6 ring carbon atoms. The ring may contain one or more carbon-carbon double bonds and the term therefore encompasses cycloalkyl and cycloalkenyl groups. Examples of carbocyclic groups include cyclohexyl, cyclopentyl and cyclohexenyl groups. Carbocyclic groups may optionally be substituted with one or more substituents independently selected from halo, methyl, ethyl, methoxy, ethoxy, nitro and cyano.
The term “heterocyclic” in the context of the present specification refers to a non-aromatic ring system having 5 or 6 ring atoms, at least one of which is a heteroatom selected form N, O and S. The ring may contain one or more double bonds. Examples of heterocyclic groups include piperidinyl, piperazinyl, morpholinyl and tetrahydrofuryl groups. heterocyclic groups may optionally be substituted with one or more substituents independently selected from halo, methyl, ethyl, methoxy, ethoxy, nitro and cyano.
The term “halo” refers to fluoro, chloro, bromo or iodo.
The term “reactive substituents” as used herein refers to a substituent which is capable of reacting with a pendant group of a solid substrate such as a membrane, nanoparticle or polymer surface or on a protein or polypeptide. There are many reactions by which compounds of formula (I) can be linked to solid substrates, and the reactive substituents will, of course, depend upon the nature of the pendant group and the chosen reaction. Suitable reactive substituents include halo, hydroxy, thiol, amino, carbonyl, carboxyl, cyano, azido, C2-C6 alkenyl and C2-C6 alkynyl groups, with particularly suitable reactive substituents being halo, hydroxyl, thiol, amino, carbonyl and carboxy.
The biological sample may be a biopsy sample, or a processed derivative thereof, taken from a patient who has or is suspected of having breast, non-small cell lung, pancreas, colon, cervical, testicular, prostate or bladder cancer. Alternatively, the biological sample may be a processed derivative of the biopsy sample, for example cells harvested from a biopsy sample, for example by centrifugation, and if necessary re-suspended in an alternative medium.
The method is particularly suitable for diagnosing prostate or bladder cancer, especially superficial bladder tumours, since cells are shed into the urine which can thus be used as the biological sample. Alternatively, a processed derivative of a urine sample may be used as the biological sample. An example of such a processed derivative is cells harvested from a urine sample and, if necessary re-suspended in an alternative medium.
In a preferred method of the invention the number of cells in said sample, or a test amount thereof, is also determined such that the NQO1/NQO2 activity can be expressed per cell. Said cells may be present in the crude sample or may be enriched, isolated or purified from the crude sample. This may be achieved by centrifugation, filtration or other methods disclosed in the scientific literature. Ideally, enrichment or purification is undertaken using a ligand binding method, such as, but without limitation, the use of paramagnetic particles coated with an antibody, receptor or other binding partner for a selected cell surface marker of interest.
In a preferred method of the invention the presence or absence of z-XH or z-X− is determined as an amount represented by a ratio of the z-XH or z-X− concentration of the sample to that of a negative assay control containing either no cells, no NQO1 and/or NQO2 expressing cells or normal cells which may because of their nature express very low levels of the enzymes. Ideally, different amounts of this ratio are correlated with known cancer cell staging techniques such that a simple in vitro assay can be used to reliably inform a clinician about, not only the existence of a cancer, but also its likely progression. In a further preferred method of the invention, greater NQO1 activity is seen in those samples from patients with later stage tumours.
In a further preferred method of the invention the assay protocol involves centrifuging a sample containing cells thought to be over-expressing NQO1 and/or NQO2, removing the supernatant, re-suspending the cells in selected buffer, incubating with a compound of general formula (I), and assaying for z-XH or z-X− as above. Ideally the incubation is undertaken for approximately 3 min. It follows that the assay of the invention can be undertaken relatively straightforwardly and takes only a short period of time, favouring a point-of-care application.
As described in greater detail below, the marker z may be a chromophore or a luminophore (e.g. a fluorescent, phosphorescent, bioluminescent or chemiluminescent marker); or a modulator of emissions from a fluorescent, phosphorescent, chemiluminescent or bioluminescent molecule or ion; or a co-factor for a chemiluminescent or bioluminescent reaction. Alternatively, the marker may be a detectable micro or nanoparticle such as, but without limitation, a coloured or magnetic particle. The method by which the presence or absence of the compound z-XH or z-X− is determined will vary depending upon the nature of the detectable marker z. For example, changes in fluorescence intensity or wavelength can be monitored using a fluorimeter and analogous changes in chemiluminescence monitored using a luminometer. If required, the product of the enzyme reaction may be isolated, for example by liquid chromatography, prior to detection and/or quantitation.
The cleaved compound z-XH or z-X− may alternatively be detectable by its ability to bind to a capture moiety and the z-XH or z-X− and capture moiety binding pairs may comprise, for example, avidin or streptavidin and biotin or an antibody/antigen binding pair such as fluorescein/anti-fluorescein.
As also described in more detail below, the cleaved compound z-XH or z-X− may alternatively be detectable by its ability to bind to a capture moiety and the z-XH or z-X− and capture moiety binding pairs may comprise, for example, avidin or streptavidin and biotin or an antibody/antigen binding pair such as fluorescein/anti-fluorescein.
The compounds of general formula (I), (Ia), (Ib), (Ic), (Id), (Ie) and (If) are substrates for NQO1 and NQO2 and are reduced by these enzymes. Reduction results in cleavage of the marker moiety z which can then be detected. Cleavage occurs according to Scheme 1 below, which illustrates the reaction mechanism for compounds of general formula (I). Reduction of compounds of general formulae (Ia), (Ib), (Ic), (Id), (Ie) and (If) proceeds in a similar manner.
The compounds of the invention are particularly useful in the detection of cancer, since the quinone moiety is a substrate for the enzymes NQO1 and NQO2, which are over-expressed in cancer cells. The compounds are particularly useful for detecting prostate and superficial bladder cancer because such cells are shed into the urine and a diagnostic test can therefore be carried out on a urine sample or on a processed derivative of a urine sample such as cells harvested from the sample, without the need for an invasive procedure. In the case where the cancer cells over-express NQO2, it may be necessary to add to the sample or processed derivative an NQO2 co-substrate such as N-ribosyldihydronicotinamide (NRH) or 1-methyl1-3-carboxamidopyridinium iodide especially when reduced to the 1,4-dihydroptridine derivative or 1-carbamoylmethyl-3-carbamoyl-1,4-dihydropyridine, all of which act as a co-substrate for NQO2. Other NQO2 co-substrates are available and known to those skilled in the art such as those described in Knox et al cancer res. 60 pp 4179-4186, 2000. It is not usually necessary to add a co-substrate in cases where the over-expression of NQO1 is to be detected since NAD(P)H, the co-substrate for NQO1, is present in all cells. However, the latter co-substrate, or its equivalent, can be used in situations where the NQO1 has previously been isolated from the biological sample prior to measurement of activity, or the cells to be investigated have been lysed.
The discussion below relates to the detection of compounds z-XH or ions z-X−. In this discussion, references to a compound of formula (I) apply also to compounds of general formulae (Ia), (Ib), (Ic), (Id), (Ie) and (If).
Suitable detectable compounds z-XH or ions z-X− include, in particular, chromophores and luminophores, for example fluorescent, phosphorescent, chemiluminescent or bioluminescent molecules or ions; or modulators of emissions from fluorescent, phosphorescent, chemiluminescent or bioluminescent molecules or ions; or a co-factor for a chemiluminescent or bioluminescent reaction. In particular, the moiety z may be chosen such that its optical properties change when it is cleaved from the remainder of the compound of general formula (I) to form the compound z-XH or the ion z-X−.
The change in optical properties may be, for example, a detectable change in the wavelength of emitted light, the removal of a quenching effect exerted by quinone moiety of general formula (I) or, in the case of co-factors, a modulation of their activity.
Most commonly, when the moiety z is a luminophore or chromophore, the change in its optical properties on cleavage from the remainder of the compound of formula (I) is based on two underlying mechanisms. The first is due to the ‘electron-withdrawing’ attachment (via the C—O—X link) of the active signalling moiety and the other is due to quenching achieved via the (pseudo) π-stacking phenomenon. These mechanisms are illustrated in Schemes 2A and 2B, using coumarin as an example.
In Scheme 2A, the coumarin anion undergoes resonance tautomerism resulting in fluorescence output. If the group X is attached to an electron withdrawing quinone moiety as in general formula (I), the fluorescence is switched off.
Scheme 2B shows pseudo π-stacking fluorescent quenching between the quinone and the aromatic coumarin moieties.
In an alternative embodiment, the moiety z may comprise a detectable label, for example a detectable particle especially a detectable micro- or nano-particle such as, but without limitation, a coloured latex microparticle, gold nanoparticle or magnetic particle, as well as numerous detectable molecules, all of which are well known to those of skill in the art. The method by which the presence or absence of the compound z-XH or ion z-X− is determined will vary depending upon the nature of the detectable moiety z. For example, changes in fluorescence intensity or wavelength can be monitored using a fluorimeter and analogous changes in chemiluminescence monitored using a luminometer. If required, the product of the enzyme reaction may be isolated, for example by liquid chromatography, prior to detection and/or quantitation.
Alternatively, the cleaved compound z-XH or ion z-X− may be detectable by its ability to bind to a capture moiety. In this case, the moiety z may simply comprise a binding portion which selectively binds the capture moiety and the capture moiety may comprise a binding partner for the moiety z and a detectable label, for example a detectable particle as described above.
When z comprises a detectable label as described above, it may further comprise a binding portion which selectively binds a capture moiety.
Examples of suitable binding pairs which may be used in this type of embodiment are known, for example biotin and either avidin or streptavidin and antigen/antibody binding pairs.
Thus, in some cases, one of z and the capture moiety may comprise biotin or a biotin derivative and the other may comprise avidin or streptavidin or a derivative thereof. Alternatively, one of z and the capture moiety may comprise an antigen and the other an antibody specific for the antigen, for example, fluorescein/anti-fluorescein. Other examples of suitable binding pairs are well known in the art.
One way of detecting such a marker is to immobilize the complex formed by the marker z and the capture moiety on a solid substrate and to detect the label on the said solid substrate. Thus, in one embodiment, the capture moiety will be immobilized on a solid substrate such as beads, fibres or a membrane and the moiety z will comprise a detectable label.
If NQO1 is present in the sample, the moiety z will be cleaved from the compound of general formula (I) and the free z-XH or z-X− will bind to the immobilized capture moiety allowing detection of the label.
Alternatively, a binding assay format may be used and this will be particularly suitable when moiety z simply comprises a binding partner for the capture moiety. In this case an appropriate labeled secondary binding reagent is used to monitor the occupancy of the capture moiety.
In some cases, the compound of formula (I) may be immobilized on a solid substrate at a first location, for example by covalent attachment involving any of the groups R1 to R5 or suitable derivatives thereof, and a capture molecule immobilized at a second location. Suitable derivatives and methods for generally immobilising molecules to solid supports are well-known to those skilled in the art. If NQO1 or NQO2 is present in the sample, the compound of formula (I) will be reduced, the moiety z will be cleaved from the residue of the compound of general formula (I) and will be free to move to the second location where it can be captured and detected.
The detection methods described above may be qualitative or quantitative. The quantitative detection of the compound z-XH or z-X− makes it possible to determine the severity of the cancer and to monitor the effectiveness of any treatment.
In a further aspect of the invention there is provided a kit for diagnosing a cancer which over-expresses NQO1 and/or NQO2, the kit comprising a composition comprising a compound of general formula (I), (Ia), (Ib), (Ic), (Id), (Ie) or (If) in a suitable container; instructions for using the kit and optionally a composition comprising an NQO2 co-substrate in a suitable container.
Specific examples of marker moieties z include fluorescein, 2-oxo-2H-1-benzopyranyl and 4-methyl-2-oxo-2H-chromen-7-yl.
In a particularly suitable embodiment, the biological sample is contacted with a compound of general formula (I).
In suitable compounds of general formula (I) independently or in any combination:
R1, R2, R3, R4 and R5 are each independently hydrogen, methyl or ethyl;
In more suitable compounds R1, R2, R3, R4 and R5 are each independently hydrogen or methyl and still more suitably:
R1 and R2 are both methyl;
R3 is hydrogen or methyl; and
R4 and R5 are the same and may be either hydrogen or methyl.
Particularly suitable compounds of general formula (I) include:
In separate alternative embodiments, the sample is contacted with a compound of general formula (Ia), a compound of general formula (Ib), a compound of general formula (Ic), a compound of general formula (Id), a compound of general formula (Ie) or a compound of general formula (If).
In the compounds of general formula (Ia), particularly suitable values for R1, R2, R3, R4, R5, X, Y and z are as defined for general formula (I), while particularly suitable values for R1′, R2′, R3′, R4′ and R5′, X′ and Y′ are as defined for R1, R2, R3, R4, R5, X and Y of general formula (I).
In compounds of general formula (Ib), particularly suitable values of R1, R2, R3, R4, R5, X, Y and z are as defined for general formula (I).
In compounds of general formula (Ic), particularly suitable values of R4, R5, X, Y and z are as defined for general formula (I);
In compounds of general formula (Id), particularly suitable values of X and z are as defined for general formula (I).
In compounds of general formula (Ie), particularly suitable values of X, Y and z are as defined for general formula (I);
In compounds of general formula (If), particularly suitable values of X and z are as defined for general formula (I) and R10 is suitably H or methyl.
In more suitable compounds of general formulae (Ic), (Id), (Ie) and (If), each of R10, R11, R12 and R13 independently represents H or C1-C6 alkyl, particularly H or methyl and most suitably H.
Compounds of general formula (I) may be prepared from compounds of general formula (II):
wherein R1, R2, R3, R4, R5 and Y are as defined for general formula (I); by reaction with a compound of formula (III):
z-XH or z-X− (III)
where X and z are as defined above for general formula (I).
Typically, this reaction is carried out in the presence of a coupling reagent such as dicyclohexylcarbodiimide (DCC) and a base such as 4-dimethylaminopyridine (DMAP). The reaction may be conducted at a temperature of about 15-30° C., typically at room temperature. When X is NR8, the reaction may be carried out in the presence of DCC and N-hydroxysuccinimide and proceeds in a similar manner to a conventional peptide coupling reaction.
Compounds of general formula (II) may be prepared from compounds of general formula (IV):
wherein R1, R2, R3, R4, R5, and Y are as defined for general formula (I);
by reaction with N-bromosuccinimide in acetonitrile, followed by the addition of water. The reaction may be conducted at a temperature of about 15-30° C., typically at room temperature.
Compounds of general formula (IV) may be prepared by reacting a compound of general formula (VI):
wherein R1, R2 and R3 are as defined for general formula (I);
with a compound of general formula (VII):
wherein Y, R4 and R5 are as defined for general formula (I).
The reaction may be conducted under acidic conditions, for example in the presence of methane sulfonic acid and at a temperature of from about 60-100° C., more usually 70-90° C.
Compounds of general formulae (VI) and (VII) are readily available or can be prepared by methods well known to those of skill in the art.
As already mentioned above, compounds of general formulae (Ia) are taught by Huang et al, Org. Letters, 8(2), 2665-268 (2006) and compounds of general formulae (Ib), (Ic), (Id) and (Ie) are discussed in Blanche et al, Tetrahedron, 65(25), 4892-4903 (2009). Methods for the preparation of these compounds are taught in these references directly or are described in references cited in those documents.
The compounds of general formula (I) and of general formulae (Ia), (Ib), (Ic), (Id), (Ie) and (If) are of use in methods for diagnosing cancer and therefore in a further aspect of the invention there is provided a compound of general formula (I) or of general formulae (Ia), (Ib), (Ic), (Id), (Ie) or (If) as defined above for use in diagnosing cancer.
There is also provided the use of a compound of general formula (I) or a compound of general formulae (Ia), (Ib), (Ic), (Id), (Ie) or (If) in the preparation of an agent for diagnosing cancer.
Examples of cancers which the compounds of general formula (I) and general formulae (Ia), (Ib), (Ic), (Id), (Ie) and (If) may be used to diagnose include breast, non-small cell lung, pancreas, colon and prostate cancers. However, the method is especially suitable for the detection of urological malignancies where tumour cells may be present in urine. Therefore, more suitably, the cancer is prostate cancer or and, still more suitably, bladder cancer, especially superficial bladder tumours.
The Invention will now be described in greater detail with reference to the Examples below and to the drawings in which:
TABLE 2 shows the assay results for patients already diagnosed with bladder cancer but as yet untreated. Results are expressed as the ratio of 4-MU concentration of the samples to that of a negative assay control. Sample 89 in this series could not be assayed due to the presence of gross haematuria.
TABLE 3 shows the NQO1 assay results for patients diagnosed with prostate carcinoma. These samples were collected following digital rectal examination.
One type of assay format is illustrated in
The invention will now be described in greater detail with reference to the examples.
Chemicals and reagents were obtained from Aldrich Chemical Co., Dorset UK, Lancaster Synthesis Ltd, Lancashire, UK and VWR International, Leicestershire, UK. Deuterated solvents and tetramethylsilane (TMS) were obtained from Cambridge Isotope Laboratories Inc., Andover USA. NQO1 was expressed and purified by Morvus Technology Ltd. Reactions were monitored using thin layer chromatography (TLC) on pre-coated 60-F254 silica gel aluminium backed plates visualised by ultra-violet (UV) radiation at 254 nm and 325 nm using a UV GL-58 mineral light lamp or by staining with potassium permanganate (KMnO4) solution. Column chromatography was carried out using silica gel 100-125 mesh from VWR international. The 1H and 13C nuclear magnetic resonance (NMR) spectra were recorded on a Bruker Avance 300 MHz or Varian 400 MHz NMR spectrometers. Chemical shifts are reported as δ parts per million (ppm) downfield of TMS for samples run in deuterated chloroform (CDCl3) or deuterated dimethyl sulfoxide (DMSO-d6). The 13C spectra were assigned with the aid of Distortionless Enhancement through Polarisation Transfer experiments (DEPT experiments). The following abbreviations were used: s (singlet), d (doublet), dd (doublet of doublets), t (triplet) and m (multiplet). J values measured in Hz. Electron ionisation (EI) and positive and negative chemical ionisation (CI) were recorded on a Micromass Trio 2000 spectrometer. Positive and negative electrospray ionisation (ESI) was recorded using a Micromass Tof Spec 2e ionisation spectrometer. High resolution spectra (HRMS) were recorded on a Thermo Finnigan MAT95XP spectrometer. Infrared spectroscopy was recorded using Jasco FT/IR-4100 running spectra manager. All peaks were reported in wavelength cm−1 Melting points (MP) were determined in open glass capillary tubes on a GallenKamp MPD.350.BM2.5 apparatus and remained uncorrected. Ultra-violet (UV) spectra were recorded on a Cary 100 UV spectrometer running Cary Win UV software using pathlength quartz cuvettes (2 opposing frosted sides), 1 cm path length. Fluorescence spectra for the enzyme reactions were recorded on a Cary Eclipse fluorimeter using 4-sided quartz thermostatted cuvettes, 1 cm path length. Slit values of 2.5, 5 and 10 were used depending on intensity generated, with an automatic shutter-on function being used to minimise photo-bleaching. The fluorescence emission and excitation spectra for chemical reductions were recorded in 4-sided quartz thermostatted cuvettes using a Shimadzu RF-5301PC spectrofluorophotometer. The light source was supplied via a 150w Xenon bulb. Data were processed using Shimadzu Rf-5301PC software. ThermoFisher Accela U-HPLC (Ultra-High Pressure Liquid Chromatography) System and separation was achieved using a C18 reverse-phase column (dimensions: 50×2.1 mm; particle size 1.9 micron). The data was analyzed using a PDA (Photo Diode Array) system and processed using ChromQuest software (version 4.2). All solvents were used without further purification. In reactions, solutions were dried with MgSO4 Solvents were evaporated under reduced pressure.
Magnetisable particles coated with anti-Ber-EP4 antibodies were obtained from (Invitrogen Dynal AS, Oslo, Norway.).
Abbreviations: DCC is dicyclohexylcarbodiimide; DMAP is 4-dimethylaminopyridine; DCM is dichloromethane; DCE is dichloroethane; DMSO is Dimethyl sulfoxide; PBS is Phosphate buffered saline.
2,3,5-Trimethyl-1,4-hydroquinone (5.0 g, 32.9 mmol) and methyl 3-3-dimethylacrylate (4.31 g, 4.94 ml, 37.8 mmol) were added to methanesulfonic acid (50 ml). The mixture was stirred at 70° C. for 3 hours and then quenched with H2O (200 ml) and extracted with ethyl acetate (3×100 mL). The organic layer was then washed with H2O (100 mL), aqueous saturated NaHCO3 (100 mL) and saturated brine (100 mL). The organic layer was then dried over MgSO4 and condensed in vacuo to give a solid. The residue was recrystallised from hexane-chloroform (3:1) to give the lactone (5.4 g, 70.2%) as colourless crystals. MP:182-184° C., lit 186-187° C. (Borchardt and Cohen, 1972b). 1H NMR (CDCl3): δ 4.73 (1H, s, OH), 2.55 (2H, s, CH2), 2.36 (3H, s, ArCH3), 2.22 (3H, s, ArCH3), 2.19 (3H, s, ArCH3), 1.45 (6H, s, 2×gem-CH3). 13C NMR (CDCl3): δ 169.3 (C═O), 148.7, 142.3, 127.6, 123.4, 120.9, 119.5 (6×ArC), 45.7 (CH2), 34.6 (C(CH3)2), 27.5 (2×gem-CH3), 14.6, 12.4, 12.1 (3×ArCH3). MS: CI 235, (M+1)+ 33%; EI 234, (M)+ 100%.
2,3-Dimethyl-1,4-hydroquinone (5.03 g, 36.2 mmol) and methyl 3-3-dimethylacrylate (5.68 mL, 4.96 g, 43.5 mmol) were added to methanesulfonic acid (50 ml). The mixture was stirred at 90° C. for 5 hours and then quenched with H2O (200 mL) and extracted into ethyl acetate (3×200 mL). The organic layer was then washed with water (100 mL), saturated NaHCO3 (100 mL) and saturated brine (100 mL). The organic layer was then dried over MgSO4 and condensed in vacuo. The residue was recrystallised from hexane-chloroform (3:1) to give the lactone as colourless crystals (3.40 g, 42.7%). MP: 145-147° C., lit: 146-148° C. (Yenes and Messeguer, 1999). 1H NMR (CDCl3): δ 6.63 (1H, s, ArH), 5.21 (1H, s, OH), 2.58 (2H, s, CH2), 2.23 (3H, s, CH3), 2.16 (3H, s, CH3), 1.30 (6H, s, 2×gem-CH3); 13C NMR (CDCl3): δ 169.4 (C═O), 150.3, 142.6, 126.5, 126.3, 122.7, 107.8 (6×ArC), 43.6 (CH2), 33.1 (C(CH3)2), 27.6 (2×gem-CH3), 12.3, 12.0 (2×ArCH3); MS: CI 221, (M+1)+ 44.7%; EI 220, (M)+ 100%.
2,3,6,-Trimethylhydroquinone (5 g, 32.9 mmol) and methyl 3-3-dimethylacrylate (3.39 g, 3.55 mL, 39.5 mmol) were added to methanesulfonic acid (50 ml). The mixture was stirred at 90° C. for 2 hours and then quenched with H2O (200 mL) and extracted with ethyl acetate (3×200 mL). The organic layer was then washed with H2O (100 mL), saturated aqueous NaHCO3 (100 mL) and saturated aqueous brine (100 mL). The organic layer was dried over MgSO4 and condensed in vacuo. The residue was recrystallised from hexane-chloroform (3:1) to give the lactone (4.21 g, 62.1%) as colourless crystals. MP: 169-171° C. 1H NMR (CDCl3): δ 4.65 (1H, s, OH), 2.91 (2H, t, CH2 J=7.2), 2.71 (2H, t, CH2 J=7.4), 2.21 (3H, s, CH3), 2.19 (3H, s, CH3), 2.18 (3H, s, CH3); 13C NMR (CDCl3): δ 169.5 (C═O), 148.2, 144.2, 122.9, 121.8, 119.3, 118.0, (6×CAr), 29.1 (3CH2), 21.3 (4CH2), 12.2, 12.1, 11.8 (3×ArCH3); CI 207, (M+1)+ 25%; EI 206, (M)+ 100%.
6-Hydroxy-4,4,5,7,8-pentamethyl-1-benzopyran-2-one (3.7 g, 15.8 mmol) was suspended in acetonitrile (aq., 15% v/v, 200 ml). A solution of NBS (3.8 g, 21.3 mmol) in acetonitrile (aq., 40% v/v, 60 ml), was added dropwise over a period of 1 hour to the suspension. The mixture was stirred for a further 30 minutes, then diluted with H2O (330 mL) and extracted with diethyl ether (3×75 mL). The combined organic phase was washed with H2O (2×100 mL) and brine (100 mL), dried over MgSO4, and then condensed at the pump to give a solid. The solid was recrystallised from ethyl acetate to afford the quinone acid as yellow crystals (1.9 g, 48.1%). MP: 97-99° C., lit: 101-103° C. (Borchardt and Cohen, 1973c). 1H NMR (CDCl3): δ 11.04 (1H, brs, OH), 3.06 (2H, s, CH2), 2.10, (3H, s, ArCH3), 1.95 (6H, s, 2×CH3), 1.43 (6H, s, 2×gem-CH3); 13C NMR (CDCl3): δ 190.9, 187.4 (2×quinone C═O), 178.9 (COOH), 152.0, 143.0, 139.0, 138.4 (4×ringC), 47.3 (CH2), 37.9 (C(CH3)2), 28.8 (2×gem-CH3), 14.3, 12.5, 12.1 (3×ArCH3); MS: CI 251, (M+1)+ 100%; EI 250, (M)+ 5%.
6-Hydroxy-4,4,7,8-tetramethyl-1-benzopyran-2-one (2.0 g, 9.1 mmol) was suspended in acetonitrile (aq., 15%, 110 mL). A solution of NBS (2.18 g, 12.3 mmol) in acetonitrile (aq., 40%, 35 mL), was added dropwise over 1 hour to the stirred lactone suspension. The mixture was stirred for a further 2 hours, then diluted with H2O (180 mL) and extracted into diethyl ether (3×45 mL). The combined organic extracts were washed with H2O (120 mL), saturated brine (100 mL) and dried over MgSO4. The yellow organic solution was evaporated to dryness and recrystallised from ethyl acetate to yield the quinone acid as yellow crystals (1.3 g, 60.5%) MP: 92-94° C. 1H NMR (CDCl3): δ 10.37 (1H, brs, OH), 6.53 (1H, s, 2-H), 2.94 (2H, s, CH2), 2.00 (6H, s, 2×CH3), 1.32 (6H, s, 2×gem-CH3); 13C NMR (CDCl3): δ 187.9, 187.5 (quinone C═O), 177.3 (COOH), 152.9, 142.4, 139.7, 132.2 (4×ringC), 44.8 (CH2), 36.8 (C(CH3)2), 28.1 (2×gemCH3), 12.6, 11.9 (2×Ar—CH3). MS: CI 237, (M+1)+ 22.4%, 254 (M+NH4) 100%; EI 237, (M+1)+ 55.3%.
The appropriate lactone (1.5 g, 7.3 mmol) was suspended in acetonitrile (aq., 15%, 90 mL). A solution of NBS (1.72 g, 9.83 mmol) in acetonitrile (aq., 40%, 30 mL), was added dropwise over 1 hour to the stirred lactone suspension. The mixture was stirred for a further 1 hour, then diluted with H2O (220 mL) and extracted with diethyl ether (3×75 mL). The combined organic extracts were washed with H2O (150 mL), dried over MgSO4, condensed in vacuo and recrystallised from ethyl acetate to yield the quinone acid as a yellow compound (1.19 g, 73.5%). MP: 113-115° C. 1H NMR (CDCl3): δ 11.00 (1H, br, OH), 2.82 (2H, t, CH2 J=7.7), 2.52 (2H, t, CH2 J=7.7), 2.11 (3H, s, CH3), 2.02 (6H, s, 2×CH3); 13C NMR (CDCl3): δ 187.5, 186.9 (quinone C═O), 178.5 (COOH), 141.8, 141.6, 140.8, 140.6 (4×ringC), 32.6 (2CH2), 22.1 (3CH2), 12.4, 12.3, 12.3 (3×Ar—CH3). MS: CI 223, (M+1)+ 100%; EI 222, (M)+ 19.7%.
A mixture of 3-(3′,6′-dioxo-2′,4′,5′-trimethylcyclohexa-1′,4′-diene)-3,3-dimethylpropanoic acid (400 mg, 1.7 mmol), DCC (415 mg, 2.0 mmol) and DMAP (21 mg, 0.2 mmol) were suspended in dry DCM (10 ml). The suspension was stirred for 30 minutes. 7-Hydroxycoumarin (317 mg, 2 Ommol) was added and the mixture was stirred for a further 24 hours. The resulting suspension was filtered and the filtrate was evaporated. The formed suspension was filtered, evaporated and redissolved in ethyl acetate and filtered. The organic extract purified by column chromatography (1:3, ethyl acetate-hexane) to afford the product: 330 mg (50.0%) as a yellow solid. MP: 128-130° C. 1H NMR (CDCl3): δ 7.68 (1H, d, ArH, J=9.6), 7.46 (1H, d, ArH, J=8.4), 7.02 (1H, d, ArH, J=2.1), 6.94 (1H, d, d, ArH, J=2.1) 6.39 (1H, d, ArH, J=9.6), 3.29 (2H, s, CH2), 2.19 (3H, s, CH3), 1.94 (6H, s, 2×CH3), 1.53 (6H, s, 2×gem-CH3). 13C NMR (CDCl3): δ 190.8, 187.3 (2×quinone C═O), 170.8 (C═OO), 160.3 (C═O coumarin), 154.7, 152.9, 151.4, 142.8, 142.7, 139.5, 138.9, 128.6, 118.3, 116.7, 116.2, 110.4 (12×ringC), 47.7 (CH2), 39.0 (C(CH3)2), 29.9 (2×gem-CH3), 14.5, 12.7, 12.2 (3×CH3). MS: ES+ve 417.1 (M+Na) 100%.
To a suspension of 3-(3′,6′-dioxo-4′,5′-dimethylcyclohexa-1′,4′-diene)-3,3-dimethylpropanoic acid (400 mg, 1.7 mmol) in dry DCM (10 mL) was added DCC (4.18 mg, 2.0 mmol) and DMAP (21 mg, 0.2 mmol). The mixture stirred for 30 minutes and then 7-hydroxycoumarin (329 mg, 2.0 mmol) was added. The mixture was then stirred for 16 hours. The resultant mixture was filtered and condensed in vacuo. The resultant solid was dissolved in ethyl acetate and filtered. The filtrate was condensed and redissolved in ethyl acetate and filtered, the resultant filtrate was again reduced in vacuo and purified by column chromatography (1:3 ethyl acetate-hexane) to yield the product as a yellow solid 285 mg (44.4%). MP: 108-110° C. 1H NMR (CDCl3): δ 7.66, (1H, d, ArH, J=9.6), 7.44 (1H, d, ArH, J=8.4), 7.00 (1H, d, ArH, J=1.8), 6.93 (1H, d,d, ArH, J=2.1, 2.1), 6.59 (1H, s, ArH), 6.38 (1H, d, ArH, J=9.6), 3.22 (2H, s, CH2), 2.02 (3H, s, CH3), 1.94 (3H, s, CH3), 1.42 (6H, s, 2×gem-CH3). 13C NMR (CDCl3): δ188.0, 187.0 (2×C═O), 170.1 (C═OO), 160.7 (C═O coumarin), 154.9, 153.2, 153.1, 143.3, 142.7, 140.3, 132.6, 129.0, 118.7, 117.1, 116.4, 110.7 (12×ringC), 45.4 (CH2), 37.7 (C(CH3)2), 28.6 (2×gem-CH3), 13.0, 12.3 (2×CH3). MS: ES+ 403.1 (M+Na).
To a solution of DCC (557 mg, 2.7 mmol) and DMAP (27 mg, 0.2 mmol) in dry DCM (10 ml) was added to 3-(3′,6′-dioxo-2′,4′,5′-trimethylcyclohexa-1′,4′-diene) propanoic acid (500 mg, 2.3 mmol) and the suspension was stirred for 30 minutes. To the mixture was added 7-hydroxycoumarin (437 mg, 2.7 mmol) and stirring was continued for 16 hours at room temperature. The resultant mixture was filtered and condensed in vacuo. The resultant solid was dissolved in ethyl acetate and filtered. The filtrate was condensed and redissolved in ethyl acetate and filtered. The resultant filtrate was again reduced in vacuo and purified by column chromatography (eluting with 1:3 ethyl acetate:hexane) to yield the product as a yellow solid 285 mg (34.6% yield). 1H NMR (CDCl3): δ 7.69 (1H, d, ArH J=9.6), 7.49 (1H, d, ArH J=8.4), 7.13 (1H, d, ArH J=2.1), 7.06, 7.04 (1H, d,d, ArH J=2.1), 6.40 (1H, d, ArH J=9.6), 2.94 (2H, t, CH2 J=7.5), 2.76 (2H, t, CH2 J=7.5), 2.11 (3H, s, CH3), 2.03 (3H, s, CH3).
A mixture of 3-(3′,6′-dioxo-2′,4′,5′-trimethylcyclohexa-1′,4′-diene)-3,3-dimethylpropanoic acid (800 mg, 3.4 mmol), DCC (830 mg, 4.0 mmol) and DMAP (42 mg, 0.4 mmol) were suspended in dry DCE (20 ml). The suspension was stirred for 30 minutes. 4-metylumberlliferone (634 mg, 3.6 mmol) was added and the mixture was stirred for a further 24 hours. The resulting suspension was filtered and the filtrate was evaporated. The formed suspension was filtered, evaporated and redissolved in ethyl acetate and filtered. The organic extract was evaporated and recrystallised from ethyl acetate to afford the product as yellow crystals (0.96 g, 69%). 1H NMR (CDCl3): δ 7.58 (1H, d, ArH, J=5 Hz), 7.02 (1H, d, ArH, J=3 Hz), 6.98 (1H, d, ArH, J=3 Hz), 6.96 (1H, d, ArH, J=3 Hz), 3.29 (2H, s, CH2), 2.41 (3H, s, CH3), 2.18 (3H, s, CH3), 1.94 (3H, s, CH3), 1.53 (6H, s, 2×CH3). 13C NMR (CDCl3): δ 190.8, 187.3 (2×quinone C═O), 170.8 (C═OO), 160.4 (C═O coumarin), 154.1, 152.8, 151.8, 151.5, 142.7, 139.7, 138.8, 125.4, 117.9, 117.8, 114.6, 110.4 (12×ringC), 47.7 (CH2), 38.4 (C(CH3)2), 29.0 (2×gem-CH3), 18.7 (CH3), 14.4, 12.6, 12.1 (3×CH3).
The following amido substrates were synthesized as models of the compounds of general formula (I) in which X is NRB.
To the appropriate quinone acid (400 mg, 1.6 mmol) was added DCC (371 mg, 1.8 mmol) and DMAP (22 mg, 0.2 mmol) in dry DCM (10 ml). The mixture was stirred for 30 mins, after which time N-methylaniline (192.6 mg, 1.8 mmol) was added. The mixture was then stirred for 16 hours. The resultant suspension was filtered and washed with HCl (aq., 0.1M, 5 ml), reduced in vacuo and purified by column chromatography (eluting with 3:1 ethyl acetate:hexane) to yield a yellow solid of the product: 390 mg (71.9%). MP: 92-94° C. 1H NMR (CDCl3): δ 7.45 (2H, t, meta H, J=6.9), 7.36 (1H, t, para H J=6.8), 7.20 (2H, d, ortho H J=7.2), 3.17 (3H, s, NCH3), 2.75 (2H, s, CH2), 2.10 (3H, s, CH3), 2.01 (3H, s, CH3), 1.97 (3H, s, CH3), 1.30 (6H, s, 2×gem-CH3). 13C NMR (CDCl3): δ 191.2 (Quinone C═O), 187.7 (Quinone C═O), 172.1 (C═O), 154.8, 144.0, 143.6, 137.7, 136.2 (5×ringC), 129.8 (meta C), 127.8 (para C), 127.5 (ortho C), 47.6 (CH2), 38.0 (C(CH3)2), 37.1 (NCH3), 28.4 (2×gem-CH3), 14.1, 12.7, 12.1 (3×CH3). MS: ES+362.2 (M+Na).
To a solution of DCC (371 mg, 1.80 mmol), and DMAP (22 mg, 0.180 mmol) in dry 10 ml DCM, 3-(3′,6′-dioxo-4′,5′-dimethylcyclohexa-1′,4′-diene)-3,3-dimethylpropanoic acid (400 mg, 1.69 mmol) was added. The mixture was stirred for 30 minutes after which time N-methylaniline (181.8 mg, 1.80 mmol) was added. The mixture was then stirred for 16 hours. The mixture was filtered and washed with HCl (aq., 0.1M, 5 ml) and reduced in vacuo and purified by column chromatography (eluting with 3:1 ethyl acetate:hexane) gave a yellow solid of the product 330 mg (60.4%). MP: 110-112° C. 1H NMR (CDCl3): δ 7.44 (2H, t, meta H J=7.4), 7.36 (1H, t, para H J=7.2), 7.16 (2H, d, meta H J=7.5), 6.49 (1H, s, quinone ArH), 3.16 (3H, s, NCH3), 2.02 (3H, s, CH3), 1.99 (3H, s, CH3), 1.16 (6H, s, 2×gem-CH3). 13C NMR (CDCl3): δ 188.2 (Quinone C═O), 188.0 (Quinone C═O), 171.3 (C═O), 155.5, 144.1, 142.3, 139.6, 130.4 (5×ringC), 129.8 (2×meta C), 127.8 (para C), 127.5 (2×ortho C), 44.4 (CH2), 37.4 (C(CH3)2), 37.2 (NCH3), 28.5 (2×gem-CH3), 12.7, 11.9 (2×Quinone CH3). MS: ES+348.1 (M+Na).
To the appropriate quinone acid (64) (400 mg, 1.8 mmol) was added DCC (371 mg, 1.8 mmol) and DMAP (22 mg, 0.2 mmol) in dry DCM (10 ml) and stirred for 30 minutes. To the stirring mixture was added N-methylaniline (192.6 mg, 1.8 mmol). The mixture was then stirred for 16 hours. The resultant mixture was filtered and washed with HCl (aq., 0.1M, 5 ml) and reduced in vacuo and purified by column chromatography (eluting with 3:1 ethyl acetate:hexane) to yield a yellow solid of the product: 285 mg (50.9%). MP: 69-71° C. 1H NMR (CDCl3): δ 7.40 (2H, t, meta H J=7.4), 7.32 (1H, t, para H J=7.2), 7.17 (2H, d, ortho H J=7.5), 3.26 (3H, s, NCH3), 2.75 (2H, t, CH2 J=7.8), 2.19 (2H, t, CH2, J=7.8) 2.00 (3H, s, 2×CH3), 1.94 (3H, s, CH3). 13C NMR (CDCl3): δ 187.6 (Quinone C═O), 186.8 (Quinone C═O), 171.7 (C═O), 143.8, 142.9, 141.0, 140.4 (5×ring C), 129.8 (2×Meta C), 127.9 (Para C), 127.3 (2×Ortho C), 37.4 (NCH3), 32.8, 23.0 (2×CH2), 12.4, 12.3, 12.1 (3×CH3). MS: ES+334.2 (M+Na).
To the appropriate quinone acid (400 mg, 1.6 mmol) was added DCC (371 mg, 1.8 mmol) and DMAP (22 mg, 0.2 mmol) in dry 10 ml DCM and stirred for 30 minutes. To the stirring mixture was added aniline (148.8 mg, 1.6 mmol). The mixture was then stirred for 16 hours at room temperature. The resultant mixture was filtered and washed with HCl (aq., 0.1M, 5 ml) and reduced in vacuo and purified by column chromatography (eluting with 3:1 ethyl acetate:hexane) to yield a yellow solid product: 410 mg (78.8%). MP: 152-154° C. 1H NMR (CDCl3): δ 7.40 (2H, d, ortho H J=8.1), 7.28 (2H, t, meta H J=7.7), 7.13 (1H, s, NH) 7.07 (1H, t, para H J=7.4), 3.02 (2H, s, CH2), 2.15 (3H, s, CH3), 1.95 (6H, s, 2×CH3), 1.50 (6H, s, 2×gem-CH3). 13C NMR (CDCl3): δ 191.6 (Quinone C═O), 187.5 (Quinone C═O), 170.2 (C═O), 153.0 (Quinone alkene C), 143.3 (Quinone alkene C), 138.3 (Quinone alkene C), 138.2 (aniline ipso C), 137.6 (Quinone alkene C), 129.0 (Meta C), 124.3 (Para C), 119.8 (Ortho C), 50.5 (CH2), 38.4 (C(CH3)2), 29.1 (2×gem-CH3), 14.2 (Quinone CH3), 12.7 (Quinone CH3), 12.2 (Quinone CH3). MS: ES+348.1 (M+Na).
To the appropriate quinone acid (400 mg, 1.69 mmol) was added DCC (371 mg, 1.80 mmol) and DMAP (22 mg, 0.180 mmol) in dry DCM (10 ml) and stirred for 30 minutes. To the stirring mixture was added aniline (157.2 mg, 1.69 mmol). The mixture was then stirred for 16 hours at room temperature. The resultant mixture was filtered and washed with HCl (aq., 0.1M, 2 ml) and reduced in vacuo and purified by column chromatography (eluting with 3:1 ethyl acetate:hexane) to yield a yellow solid (86) 390 mg (73.9%). MP: 131-133° C. 1H NMR (CDCl3): δ 7.37 (2H, d, ortho H J=7.5), 7.27 (2H, t, meta H, J=7.8), 7.07 (2H, t, para H, J=7.4) 7.05 (NH, D2O exchange), 6.60 (1H, s, quinone H), 2.94 (2H, s, CH2), 2.02 (3H, s, CH3), 2.00 (3H, s, CH3), 1.38 (6H, s, 2×gemCH3). 13C NMR (CDCl3): δ 188.4 (quinone carbonyl C═O), 187.9 (quinone carbonyl C═O), 169.1 (amide carbonyl C═O), 153.5 (quinone C), 142.3 (quinone C), 140.0 (quinone C), 137.4 (aniline ipso C), 132.0 (quinone C), 129.0 (meta C), 124.4 (para C), 119.8 (ortho C), 48.3 (CH2), 37.6 (C(CH3)2), 28.5 (2×gemCH3), 12.6 (quinone CH3), 11.9 (quinone CH3). MS: ES+334.1 (M+Na).
To the appropriate quinone acid (400 mg, 1.80 mmol) was added DCC (371 mg, 1.80 mmol) and DMAP (22 mg, 0.180 mmol) in dry DCM (10 ml) and stirred for 30 minutes. To the stirring mixture was added aniline (167.4 mg, 1.80 mmol). The mixture was then stirred for 16 hours at room temperature. The resultant mixture was filtered and washed with HCl (aq., 0.1M, 2 ml) and reduced in vacuo and purified by column chromatography (eluting with 3:1 ethyl acetate:hexane) to yield a yellow solid of the product: 360 mg (67.2%). MP: 135-137° C. 1H NMR (CDCl3): δ 8.09 (1H, s, NH), 7.51 (2H, d, ortho H J=7.8), 7.30 (2H, t, meta H J=8.0), 7.08 (1H, t, para H J=7.4), 2.89 (2H, t, CH2 J=7.7), 2.50 (2H, t, CH2, J=7.8), 2.08 (3H, s, CH3), 2.00 (6H, s, 2×CH3). 13C NMR (CDCl3): δ 187.4, 187.4 (2×C=Oquinone), 170.0 (C═O), 142.2, 141.7, 141.0, 140.4, 137.8 (5×ringC), 129.0 (meta C), 124.3 (para C), 119.8 (ortho C), 36.2 (CH2), 23.0 (CH2), 12.5, 12.3 (3×CH3). MS: ES+ 320.2 (M+Na) 100%.
NMP Solubility: A solution of the product of Example 3D (MTL8-252) was prepared in NMP and further diluted to 0.1M in NMP. The purity of the compound was assessed via uplc using a 1-99% Acetonitrile gradient. Injection volumes of 0.5 μl, 1 μl, 2 μl, 3 μl and 5 μl were used. Purity was determined to be almost 100% based on uv data at 265 nm. The FL Plus settings were (high PMT voltage, λex 410 nm and λem 550 nm, off peak). Only a single peak with retention time of approx 4.3 min was detected. MTL8-252 was equally very soluble in DMSO.
Aqueous solutions of NMP and DMSO stocks in 10 mM sodium phosphate buffer, pH 10 resulted in a slightly cloudy solution that did not pellet when spun at 13000 rpm in a bench microfuge. However, the peak area from UV hplc trace did not appear to have been altered (compared to that of NMP diluted sample). Filtration using a 0.2 micron membrane resulted in a clear solution but no peak was detected on hplc, suggesting that the material was retained on the nylon filter. A similar result was obtained for the diluted DMSO stock. Subsequent experiments utilised 0.1M diluted stock in 10 mM phosphate buffer, pH 7.0 and injected directly onto the uplc column
Stability of 0.1M MTL8-252 in NMP was assessed over a 2-hour period at 37° C. The compound appeared stable as evidenced by the fact that the uv absorbance peak area remained substantially unchanged. No detectable release of 4-methylumbelliferone (4-MU) was observed. A similar experiment was carried out with DMSO stock diluted in 10 mM sodium phosphate buffer. The result (see
Initial HPLC was carried out on samples containing MTL8-252 (0.1 mM), NADH (0.5 mM) and 0.5 μg/ml hNQO1 in phosphate buffer (10 mM, pH 7) using a 1-99% acetonitrile gradient and a 1 μl injection volume. Temperature was maintained at 37° C. The results are shown in Table 1 below.
Reduction of the parent compound was rapid as most of it had disappeared by the second injection. An enzyme concentration of 0.2 μg/ml hNQO1 was found to be suitable for determining the initial rate of MTL8-252 reduction. No significant reduction in the area of the peak was observed in the presence of either MTL8-252 alone or with NADH alone (see
This finding demonstrates that MTL8-252 is a substrate for hNQO1. There was an increase in 4-MU peak area when monitored at 325 nm that matched the loss of substrate. This was confirmed by an increase in 4-MU fluorescence peak. The presence of NADH did not interfere with the FL spectra.
The cut off point for the UV data was initially set between 220-800 nm and when the lower wavelength was reduced to 180 nm, the lactone, 4-MU and MTL8-252 were quite evident despite minimal baseline noise interference.
By altering the settings of the FL Plus detector (Low PMT voltage, λex 410 nm, λem 550 nm) and calibrating for 4-MU measurements by establishing a calibration graph it can be seen that there was a linear response to 4-MU up to at least 200 μM. The maximum concentration used in incubation experiments is 100 μM, final.
Following calibration of the instrument, a repeat experiment of the activation of MTL8-252 by hNQO1 was carried out resulting in calculated rates of loss of MTL8-252 and formation of 4-MU (see
Replots of UV data at 210 nm, showed initial rates of formation of 4-MU and lactone were 11.48 uM/min and 9.84 uM/min, respectively (see
This experiment was performed to ensure the products of activation were not further metabolised by hNQO1. HPLC was carried out on samples containing 4-MU and Lactone (0.1 mM), NADH (0.5 mM) and hNQO1 (0.2 μg) in phosphate buffer (10 mM, pH 7) using a 1-99% acetonitrile gradient and a 2 μl injection volume. Temperature was maintained at 37° C. The results show both by products released following activation of MTL8-252 were not substrates of hNQO1 (see
Human NQO1 expressing cells (HDT7) and null expressing cells (F179) were cultured in Eagle's MEM medium enriched with non-essential amino acids and 10% FBS. Cells were harvested in exponential growing phase and suspended in cold PBS @ 5×106 cells/ml. 100 ul of cell suspension (5×105 cells) were added to 3 ml PBS containing 10 uM MTL8-252 at 37° C. in a fluorimeter cuvette and the rate of increase in fluorescence intensity (attributed to 4-MU release), measured over 3 minutes. Control experiment contained substrate alone in PBS.
The Luminescence Spectrophotometer settings were as follows: λex=360 nm; λem=452 @ 37° C.
Cells in exponential growth phase were harvested and resuspended in cold PBS at 5×106 cells/ml. 100 ul of cell suspension (5×105 cells) were added to 1 ml PBS containing 10 uM MTL8-252 at 37° C. and incubated for the following time periods: 0, 5, 30 and 60 min. The reaction was stopped by spinning down cells at 13,000 rpm for 30 seconds and the cell free extract injected (2 ul) directly unto the uplc. Separation of metabolites was achieved using a Whatman Partisil C18 column (S/N no. 4SF03177) with a gradient of 1-99% MeCN over 10 min at a flow rate of lml/min FL-Plus detector settings were as follows: PMT (low), λex=360 nm; λem=450 nm
Replacement of PBS with cell culture medium suggests other components in the media can reduce MTL8-252,
The following general method was adopted for the results shown in
The standard method described above was performed on replicate samples of NQO1-producing HDT7 cells added into culture medium or urine.
The method was also used to assay various cell types for NQO1 activity, namely HDT7 and F179 (NQO1-producing and null engineered cell lines respectively), human bladder cancer cell lines EJ138 and RT112 and human prostate cancer cell line PC3. The results are shown in
Prostate carcinoma cells (as exemplified by PC3 cells) express both NQO1 and NQO2 activity. However, the latter is only activated by the presence of a co-substrate not naturally present in the cells (EP-0152R, 1-carbamoylmethyl-3-carbamoyl-1,4-dihydronicotinamide). This was demonstrated as follows:
To 440 ul of PBS containing 5×105 PC-3 cells pre-incubated at 37° C. for 5 min was added 10 uM MTL8-252 fluorogenic substrate in DMSO (final, 10 ul) and volume adjusted to 500 ul with PBS. After incubating for a further 5 min the mixture was syringe filtered as above and 2 ul of supernatant analysed by uplc as described earlier. The reaction on the same number of cells was repeated with the inclusion of 100 uM EP-0152R (50 ul) as co-substrate during incubation. The reaction mixture was filtered and analysed as above. It is known that in the absence of EP-152R hNQO2 is inactive.
Various numbers of RT112 cells were subjected to the above assay to demonstrate the quantitation capabilities of the method. The data for these tests are shown in
In certain situations it is advantageous to improve sensitivity and/or specificity of the method by enrichment/isolation/purification of cells prior to assay. This example demonstrates the ability and compatibility of the present invention with such procedures. Here, samples containing cancer cells carrying the Ber-EP4 epithelial antigen were isolated using magnetisable particles coated with antibodies to this antigen (Invitrogen). The particles were used according to the Manufacturer's instructions such that a suspension of particle/cell complexes was obtained which was processed in the standard assay protocol described above.
Urine samples (20 ml) were obtained from patients attending a urology clinic and subjected to the assay outlined above. Results are expressed as the ratio of 4-MU concentration (produced from the fluorogenic substrate as the result of NQO1 activity) of the samples to that of the negative assay control and correlated with the eventual clinical diagnosis (transitional cell carcinoma, or not). The open triangle represents a sample containing significant amounts of debris suggesting that the use of immunoextraction as described herein would be beneficial for increasing specificity. The open circle represents a sample from a patient subsequently diagnosed with very early stage Ta bladder cancer and it is likely that there were insufficient cells for reliable analysis of this sample. The chemiluminogenic substrates described herein are likely to be advantageous in situations where higher sensitivity of detection is required.
Table 2 shows the corresponding results for patients already diagnosed with bladder cancer but as yet untreated. Sample 89 in this series could not be assayed due to the presence of gross haematuria.
Further urine samples (20 ml) were collected from patients diagnosed with prostate carcinoma. These samples were collected following digital rectal examination. Table 3 shows the results of the NQO1 assay for these samples. In both of these experiments, greater NQO1 activity was seen in those samples from patients with later stage tumours. In the method of the invention the quantitative increase in NQO1/NQO2 activity with advanced disease means the method can be used, not only to identify cancer, but also to assess the progress of the disease by relating the quantitative nature of the assay to the various stages of the disease. Those skilled in the art will appreciate this is possible because of the sensitivity of the assay and the pathology of the disease.
| Number | Date | Country | Kind |
|---|---|---|---|
| 1021494.8 | Dec 2010 | GB | national |
| Filing Document | Filing Date | Country | Kind | 371c Date |
|---|---|---|---|---|
| PCT/GB2011/052430 | 12/8/2011 | WO | 00 | 7/9/2013 |
