METHODS AND DEVICES FOR THE SELECTIVE DETECTION OF MICROORGANISMS

STATEMENT OF GOVERNMENTAL SUPPORT

[Not Applicable]

FIELD OF THE INVENTION

The present invention relates to field of assays and diagnostics. In particular assays methods and devices are provided for the rapid and specific detection of target microorganisms, cells, and the like.

BACKGROUND OF THE INVENTION

Various bacteria are responsible for numerous human diseases. For example, Escherichia coli can cause several intestinal and extra-intestinal infections such as urinary tract infections, meningitis, peritonitis, mastitis, septicemia and Gram-negative pneumonia. Bacterial infections from Mycoplasma pneumoniae, may lead to tracheobronchitis, primary atypical pneumonia, contribute to the onset and exacerbation of asthma, and other respiratory disorders. Infections from Mycoplasma genitalium may lead to urogenital disease. Bacterial infections, such as these noted above, are the cause of millions of hospitalizations and thousands of deaths each year. Other infections impact the food and agriculture industries. For example, Mycoplasma gallisepticum (MG) causes severe chronic respiratory disease in chickens and turkeys resulting in hundreds of millions of dollars in annual losses to the poultry industry in the US alone.

Classical microbiological methods are still the most commonly used techniques for identifying and quantifying specific bacterial pathogens. These methods are generally easy to perform, do not require expensive supplies or laboratory facilities, and offer high levels of selectivity; however, they are slow. Classical microbiological methods, however, are hindered by the requirement to first grow or cultivate pure cultures of the targeted organism, which can take many hours to days. This time constraint severely limits the ability to provide a rapid and ideal response to the presence of virulent strains of microorganisms. The extensive time it takes to identify microorganisms using standard methods is a serious problem resulting in significant human morbidity and increased economic costs.

Molecular biology techniques are quickly gaining acceptance as valuable alternatives to standard microbiological tests. Serological methods have been widely employed to evaluate a host of matrices for targeted microorganisms (see, e.g., Kingsbury and Falkow (1985) Rapid Detection And Identification of Infectious Agents, Academic Press, Inc., New York). These tests focus on using antibodies to first trap and then separate targeted organisms from other constituents in complicated biological mixtures. Once isolated, the captured organism can be concentrated and detected by a variety of different techniques that do not require cultivating the biological analyte. One such approach, termed “immunomagnetic separation” (IMS), involves immobilizing antibodies to spherical, micro-sized magnetic or paramagnetic beads and using these beads to trap targeted microorganisms from liquid media. The beads are easily manipulated under the influence of a magnetic field facilitating the retrieval and concentration of targeted organisms.

Detection methods previously used with IMS include, for example, ELISA (Kofitsyo et al. (1995) Int. J. Food Microbiol., 27: 11-25), dot blot assays (Skjerve et al. (1990) Appl. & Env. Microbiol., 3478-3481), electrochemiluminescence (Yu and Bruno (1996) Appl. & Env. Microbiol., 587-592), and flow cytometry (Pyle et al. (1999) Appl. & Env. Microbiol., 1966-1972). Although these tests provide satisfactory results, they are laborious to perform and give binary responses (yes/no) that are highly susceptible to false-positive results due to cross-reactivity with non-target analytes. While these approaches can offer faster results than do traditional microbiology methods, they do not typically achieve the sensitivity levels that substrate-based assays do, are more expensive, and typically require more highly trained technicians than do classical substrate-based methods.

Other molecular biology techniques that have received a great deal of attention recently are Polymerase Chain Reaction (PCR) methods. PCR detection of specific microorganisms in a sample involves extraction of the genetic material (RNA and/or DNA) in a sample, amplification of a target genetic sequence specific to the microorganism of interest, and then detection of the amplified genetic material. PCR techniques offer high selectivity owing to the uniqueness of the detected genetic material, high sensitivity because of the substantial amplification of the target genetic material, and rapid results owing to the potentially fast amplification process. However, PCR instruments and reagents are quite expensive and highly trained technicians are needed to perform the tests. In addition, numerous steps are involved that increase the chance of errors.

SUMMARY OF THE INVENTION

In various embodiments methods and devices are provided for the rapid and specific detection of target microorganisms, cells, and the like. In one embodiment, the methods involve contacting a target microorganism (e.g., in a sample) with a selective permeabilization reagent that selectively permeabilizes or lyses the target microorganism; contacting the selectively permeabilized or lysed microorganism with a detection reagent that is taken into the selectively permeabilized organism or that contacts metabolites or enzymes released by the selectively permeabilized microorganism, where the detection reagent produces a signal in the presence of said metabolites or enzymes; and detecting a signal produced by the detection reagent in the presence of the metabolites or enzymes wherein the strength of the signal indicates the presence or amount of the target microorganism.

In another illustrative embodiment, the method involves contacting the target microorganism with a permeabilization reagent that selectively permeabilizes the target microorganism; contacting the selectively permeabilized target microorganism with a cell-impermeant label; and detecting said label in the microorganism (cell) where the presence or amount of said label associated with a microorganism indicates the presence or amount of the target microorganism.

In certain embodiments the methods provide methods of detecting or quantifying a target microorganism in a sample are provided. The methods typically involve contacting the target microorganism with a selective permeabilization reagent that selectively permeabilizes or lyses the microorganism; contacting the selectively permeabilized microorganism with a detection reagent that is taken into the selectively permeabilized organism or that contacts metabolites or enzymes released by the selectively permeabilized microorganism, where the detection reagent produces a signal in the presence of the metabolites or enzymes; and detecting a signal produced by the detection reagent in the presence of the metabolites or enzymes where the strength of the signal indicates the presence and/or amount of the target microorganism in the sample. In certain embodiments the metabolites or enzymes comprise a metabolite or enzyme selected from the group consisting of ATP, DNA, RNA, calcium, beta-galactosidase (beta-gal), beta-glucuronidase, alcohol dehydrogenase or other NAD oxidoreductase, a transferase, an alkaline phosphatase or other hydrolase, a lyase, an isomerase, an oxidase, a gyrase, a DNA nuclease (DNases), and 1RNA nuclease (RNase), and a restriction enzyme. In certain embodiments the metabolites or enzymes comprise ATP. In certain embodiments the detection reagent comprises a luciferase and the signal comprises a luminescence signal. In certain embodiments the detection reagent comprises a target responsive electrochemical aptamer switch (TREAS) for ATP detection and the signal comprises an electrochemical signal. In certain embodiments the detection reagent comprises a molecular beacon (MB)-like DNA for the detection of ATP and the signal comprises a fluorescent signal. In certain embodiments the detection reagent comprises an enzyme substrate (e.g., beta-galactosidase (beta-gal), beta-glucuronidase, alcohol dehydrogenase or other NAD oxidoreductases, transferases, alkaline phosphatases or other hydrolases, lyases, isomerases, oxidases, gyrases, a DNA nuclease (DNases), and 1RNA nuclease (RNase), a restriction enzyme, and the like) and the detecting comprises detecting a reaction between the released enzyme and the enzyme substrate. In certain embodiments the substrate is selected from the group consisting of coumarin-4-acetic acid 7-O-caprylate, coumarin-4-acetic acid 7-O-beta-D-glucuronide, and coumarin-4-acetic acid 7-O-beta-D-galactopyranoside. In certain embodiments the detection reagent comprises an enzyme (e.g., an enzyme that uses NAD, NADP, or FAD as a cofactor) and a substrate for that enzyme and the detecting comprises detecting the reaction between the enzyme and the substrate in the presence of a cofactor or a coenzyme that is released from the microorganism. In various embodiments the enzyme substrate and/or the enzyme is provided on and/or in a solid support. In certain embodiments the substrate comprises glucose or another substrate for glucose oxidase, and glucose dehydrogenase. In certain embodiments the detecting comprises detecting the reduction of one or more coenzymes selected from the group consisting of NAD, NADP, and FAD. In certain embodiments the substrate comprises hexokinase, a hexose, glucose-6-phosphate dehydrogenase, and NAD. In certain embodiments the detecting comprises detecting released ATP by detecting the reduction of the NAD to NADH. In certain embodiments the substrate comprises glucose-6-phosphate dehydrogenase. In certain embodiments the detecting comprises detecting released NAD by detecting the reduction of the NAD to NADH. In various embodiments the detection of the reduction of NAD NADP, or FAD comprises detection of a colorimetric reagent that changes color when oxidized or reduced. In certain embodiments the detection of the reduction of NAD NADP or FAD comprises electrochemical detection of a reagent that is oxidized or reduced. In certain embodiments the substrate comprises a test strip compatible with a glucometer readout device. In certain embodiments the test strip comprises a calibration code. In various embodiments the contacting the target microorganism with a selective permeabilization reagent occurs on and/or in the substrate (support). In certain embodiments the contacting the target microorganism with a selective permeabilization reagent occurs in a sample collection device before application to the substrate.

In certain embodiments methods of detecting or quantifying a target microorganism in a sample are provided where the methods involve contacting the target microorganism with a permeabilization reagent that selectively permeabilizes the microorganism; contacting the selectively permeabilized microorganism with a cell-impermeant label; and detecting the label in the cell where the presence or amount of the label associated with a microorganism indicates the presence or amount of the target microorganism in the sample. In certain embodiments the detecting comprises a method selected from the group consisting of microscopy, flow cytometry, solid phase cytometry, luminometry, and spectroscopy. In certain embodiments the impermeant label comprises a label selected from the group consisting of propidium iodide, SYTOX Green, SYBR®-14, YoYo®-1, YO-PRO™-1, BO-PRO-1, PO-PRO-1, YO-PRO-1, TO-PRO-1, TO-PRO-3, BO-PRO-3, YO-PRO-3, TO-PRO-#, POPO-1, BOBO-1, YOYO-1, TOTO-1, POPO-3, BOBO-2, YOYO-3, TOTO-3, ethidium homodimers-1, ethidium homodimers-2, ethidium bromide, ethidium monoazide, and Trypan blue. In certain embodiments the detecting comprises a method selected from the group consisting of microscopy, flow cytometry, solid phase cytometry. In various embodiments the methods further involve concentrating the microorganisms before detecting the label.

In certain embodiments of any one of the foregoing methods, the permeabilization reagent comprises a reagent that disrupts or permeabilizes a microorganism or cell (e.g., an antimicrobial peptide) attached to a targeting peptide or antibody that preferentially or specifically binds to the target microorganism. In certain embodiments the targeting peptide preferentially or specifically binds to a target microorganism selected from the group consisting of Acinetobacter baumannii, Actinomyces naeslundii, Aspergillus niger, Bacteroides fragilis, Bacillus subtilis, Candida albicans, Clostridium difficile, Corynebacterium jeikeium, Campylobacter jejuni, Escherichia coli, Enterococcus faecalis, Fusobacterium nucleatum, Lactobacillus acidophilus, Legionella pneumophila, Micrococcus luteus, Mycobacterium smegmatis, Malassezia furfur, Methicillin-resistant Staphylococcus aureus (MRSA), Myxococcus xanthus, Pseudomonas aeruginosa, Porphyromonas gingivalis, Progeussmirabilis, S. epidermidis, Streptococcus mutans, Streptococcus pneumoniae, Treponema denticola, and Trichophyton rubrum. In certain embodiments the targeting peptide is a targeting peptide selected from the targeting peptides listed in Table 2. In certain embodiments the targeting peptide is attached directly or indirectly (e.g. via a linker) to an antimicrobial peptide. In certain embodiments the antimicrobial peptide is an antimicrobial peptide selected from the antimicrobial peptides listed in Table 4. In various embodiments the target microorganism is S. mutans, and the targeting peptide attached to an antimicrobial peptide comprises an amino acid sequence selected from the group consisting of TFFRLFNRSFTQALGKGGGKNLRIIRKGIHIIKKY (C16G2, SEQ ID NO:1117), KFINGVLSQFVLERKPYPKLFKFLRKHLL (1845L621, SEQ ID NO:1118), FIDSFIRSFGGGKLFKFLRKHLL (b43BD2.21, (SEQ ID NO:1119), TFFRLFNRSFTQALGKGGGFLKFLKKFFKKLKY (C16AF5, (SEQ ID NO:1120), and FIKHFIHRFGGGKNLRIIRKGIHIIKKY (2_—1G2, (SEQ ID NO:1121). In certain embodiments the targeting peptide attached to an antimicrobial peptide comprises an amino acid sequence selected from the group consisting of KKHRKHRKHRKH GGSGGS KNLRRIIRKGIHIIKKYG (G10KHc, (SEQ ID NO:1122). In various embodiments of any one of the foregoing methods the method is performed in a well of a multi-well plate. In certain embodiments different wells of the multi-well plate contain permeabilization reagents that selectively permeabilize different microorganisms. In any one of the foregoing embodiments, the sample comprises a sample from saliva, plaque, urine, feces, cerebrospinal fluid, blood, vaginal secretions, soil, a surface swab, an agricultural product, a meat product, a poultry product, and a fish product.

In various embodiments a diagnostic test device is provided. The device typically comprises a substrate test strip comprising a selective permeabilization reagent; an enzyme substrate; and a detection reagent that detects a change in oxidation state of a coenzyme. In certain embodiments the substrate comprises glucose or another substrate for glucose oxidase, and glucose dehydrogenase. In certain embodiments substrate comprises one or more coenzymes selected from the group consisting of NAD and FAD. In certain embodiments the substrate comprises hexokinase, a hexose, glucose-6-phosphate dehydrogenase, and NAD. In certain embodiments the substrate comprises glucose-6-phosphate dehydrogenase. In certain embodiments the detection reagent comprises a colorimetric reagent that changes color when oxidized or reduced. In certain embodiments the detection reagent that is detectable using an electrochemical detection device. In certain embodiments substrate comprises a test strip compatible with a glucometer readout device. In certain embodiments the test strip comprises a calibration code.

In certain embodiments a diagnostic test unit is provided. The test unit typically comprises a swab member carried by a housing base defining a sample chamber a housing cap comprising a first reagent chamber where the housing cap interfits with the housing base to cooperatively form a capped sample chamber with the swab disposed therein and a break-off nib, channel, or port that communicates between the first reagent chamber and the sample chamber; and a permeabilization reagent that selectively permeabilizes or lyses a target microorganism where the permeabilization reagent is disposed within the first reagent chamber or within the sample chamber. In certain embodiments the first reaction chamber further contains a detection reagent. In certain embodiments the housing cap or the housing base comprises a second reagent chamber containing a detection reagent. In certain embodiments the sample chamber contains a detection reagent. In certain embodiments the detection reagent comprises an enzyme substrate or a luciferase. In certain embodiments permeabilization reagent comprises a reagent that disrupts or permeabilizes a microorganism attached to a targeting peptide that preferentially or specifically binds to the target microorganism. In certain embodiments the targeting peptide preferentially or specifically binds to a target microorganism selected from the group consisting of Acinetobacter baumannii, Actinomyces naeslundii, Aspergillus niger, Bacteroides fragilis, Bacillus subtilis, Candida albicans, Clostridium difficile, Corynebacterium jeikeium, Campylobacter jejuni, Escherichia coli, Enterococcus faecalis, Fusobacterium nucleatum, Lactobacillus acidophilus, Legionella pneumophila, Micrococcus luteus, Mycobacterium smegmatis, Malassezia furfur, Methicillin-resistant Staphylococcus aureus (MRSA), Myxococcus xanthus, Pseudomonas aeruginosa, Porphyromonas gingivalis, Progeussmirabilis, S. epidermidis, Streptococcus mutans, Streptococcus pneumoniae, Treponema denticola, and Trichophyton rubrum. In certain embodiments targeting peptide is a targeting peptide selected from the targeting peptides listed in Table 2. In certain embodiments the targeting peptide is attached directly or indirectly to an antimicrobial peptide. In certain embodiments the antimicrobial peptide is an antimicrobial peptide selected from the antimicrobial peptides listed in Table 4. In certain embodiments the target microorganism is S. mutans, and the targeting peptide attached to an antimicrobial peptide comprises an amino acid sequence selected from the group consisting of TFFRLFNRSFTQALGKGGGKNLRIIRKGIHIIKKY (C16G2, SEQ ID NO:1129), KFINGVLSQFVLERKPYPKLFKFLRKHLL (1845L621, SEQ ID NO:1130), FIDSFIRSFGGGKLFKFLRKHLL (b43BD2.21, (SEQ ID NO:1131), TFFRLFNRSFTQALGKGGGFLKFLKKFFKKLKY (C16AF5, (SEQ ID NO:1132), and FIKHFIHRFGGGKNLRIIRKGIHIIKKY (2_—1G2, (SEQ ID NO:1133). In certain embodiments the targeting peptide attached to an antimicrobial peptide comprises an amino acid sequence selected from the group consisting of KKHRKHRKHRKH GGSGGS KNLRRIIRKGIHIIKKYG (G10KHc, (SEQ ID NO:1134).

In certain embodiments in any of the foregoing methods, diagnostic devices, or diagnostic test units, the selective permeabilization reagent comprises one of the targeting peptides selected from Table 2 attached to an antimicrobial peptide (AMP). Such “STAMPS” include, for example, 1T-3 attached to an AMP, 1T-4 attached to an AMP, 1T-6 attached to an AMP, 1T-7 attached to an AMP, 1T-8 attached to an AMP, 1T-9 attached to an AMP, 1T-10 attached to an AMP, 1T-11 attached to an AMP, 1T-12 attached to an AMP, 1T-13 attached to an AMP, 1T-14 attached to an AMP, 1T-15 attached to an AMP, 1T-16 attached to an AMP, 1T-17 attached to an AMP, 1T-18 attached to an AMP, 1T-19 attached to an AMP, 1T-20 attached to an AMP, 1T-21 attached to an AMP, 1T-22 attached to an AMP, 1T-23 attached to an AMP, 1T-24 attached to an AMP, 1T-25 attached to an AMP, 1T-26 attached to an AMP, 1T-27 attached to an AMP, 1T-28 attached to an AMP, 1T-29 attached to an AMP, 1T-30 attached to an AMP, 1T-31 attached to an AMP, 1T-32 attached to an AMP, 1T-33 attached to an AMP, 1T-34 attached to an AMP, 1T-35 attached to an AMP, 1T-36 attached to an AMP, 1T-37 attached to an AMP, 1T-38 attached to an AMP, 1T-39 attached to an AMP, 1T-40 attached to an AMP, 1T-41 attached to an AMP, 1T-42 attached to an AMP, 1T-43 attached to an AMP, 1T-44 attached to an AMP, 1T-45 attached to an AMP, 1T-46 attached to an AMP, 1T-47 attached to an AMP, 1T-48 attached to an AMP, 1T-49 attached to an AMP, 1T-50 attached to an AMP, 1T-51 attached to an AMP, 1T-52 attached to an AMP, 1T-53 attached to an AMP, 1T-54 attached to an AMP, 1T-55 attached to an AMP, 1T-56 attached to an AMP, 1T-57 attached to an AMP, 1T-58 attached to an AMP, 1T-59 attached to an AMP, 1T-60 attached to an AMP, 1T-61 attached to an AMP, 1T-62 attached to an AMP, 1T-63 attached to an AMP, 1T-64 attached to an AMP, 1T-65 attached to an AMP, 1T-66 attached to an AMP, 1T-67 attached to an AMP, 1T-68 attached to an AMP, 1T-69 attached to an AMP, 1T-70 attached to an AMP, 1T-71 attached to an AMP, 1T-72 attached to an AMP, 1T-73 attached to an AMP, 1T-74 attached to an AMP, 1T-75 attached to an AMP, 1T-76 attached to an AMP, 1T-77 attached to an AMP, 1T-78 attached to an AMP, 1T-79 attached to an AMP, 1T-80 attached to an AMP, 1T-81 attached to an AMP, 1T-82 attached to an AMP, 1T-83 attached to an AMP, 1T-84 attached to an AMP, 1T-85 attached to an AMP, 1T-86 attached to an AMP, 1T-87 attached to an AMP, 1T-89 attached to an AMP, 1T-90 attached to an AMP, 1T-91 attached to an AMP, 1T-92 attached to an AMP, 1T-93 attached to an AMP, 1T-94 attached to an AMP, 1T-95 attached to an AMP, 1T-96 attached to an AMP, 1T-97 attached to an AMP, 1T-98 attached to an AMP, 1T-99 attached to an AMP, 1T-100 attached to an AMP, 1T-101 attached to an AMP, 1T-102 attached to an AMP, 1T-103 attached to an AMP, 1T-104 attached to an AMP, 1T-105 attached to an AMP, 1T-106 attached to an AMP, 1T-107 attached to an AMP, 1T-108 attached to an AMP, 1T-109 attached to an AMP, 1T-110 attached to an AMP, 1T-111 attached to an AMP, 1T-112 attached to an AMP, 1T-113 attached to an AMP, 1T-114 attached to an AMP, 1T-115 attached to an AMP, 1T-116 attached to an AMP, 1T-117 attached to an AMP, 1T-118 attached to an AMP, 1T-119 attached to an AMP, 1T-120 attached to an AMP, 1T-121 attached to an AMP, 1T-122 attached to an AMP, 1T-123 attached to an AMP, 1T-124 attached to an AMP, 1T-125 attached to an AMP, 1T-126 attached to an AMP, 1T-127 attached to an AMP, 1T-128 attached to an AMP, 1T-129 attached to an AMP, 1T-130 attached to an AMP, 1T-131 attached to an AMP, 1T-132 attached to an AMP, 1T-133 attached to an AMP, 1T-134 attached to an AMP, 1T-135 attached to an AMP, 1T-136 attached to an AMP, 1T-137 attached to an AMP, 1T-138 attached to an AMP, 1T-139 attached to an AMP, 1T-140 attached to an AMP, 1T-141 attached to an AMP, 1T-142 attached to an AMP, 1T-143 attached to an AMP, 1T-144 attached to an AMP, 1T-145 attached to an AMP, 1T-146 attached to an AMP, 1T-147 attached to an AMP, 1T-148 attached to an AMP, 1T-149 attached to an AMP, 1T-150 attached to an AMP, 1T-151 attached to an AMP, 1T-152 attached to an AMP, 1T-153 attached to an AMP, 1T-154 attached to an AMP, 1T-155 attached to an AMP, 1T-156 attached to an AMP, 1T-157 attached to an AMP, 1T-158 attached to an AMP, 1T-159 attached to an AMP, 1T-160 attached to an AMP, 1T-161 attached to an AMP, 1T-162 attached to an AMP, 1T-163 attached to an AMP, 1T-164 attached to an AMP, 1T-165 attached to an AMP, 1T-166 attached to an AMP, 1T-167 attached to an AMP, 1T-168 attached to an AMP, 1T-169 attached to an AMP, 1T-170 attached to an AMP, 1T-171 attached to an AMP, 1T-172 attached to an AMP, 1T-173 attached to an AMP, 1T-174 attached to an AMP, 1T-175 attached to an AMP, 1T-176 attached to an AMP, 1T-177 attached to an AMP, 1T-178 attached to an AMP, 1T-179 attached to an AMP, 1T-180 attached to an AMP, 1T-181 attached to an AMP, 1T-182 attached to an AMP, 1T-183 attached to an AMP, 1T-184 attached to an AMP, 1T-185 attached to an AMP, 1T-188 attached to an AMP, 1T-190 attached to an AMP, 1T-192 attached to an AMP, 1T-193 attached to an AMP, 1T-195 attached to an AMP, 1T-196 attached to an AMP, 1T-197 attached to an AMP, 1T-198 attached to an AMP, 1T-199 attached to an AMP, 1T-200 attached to an AMP, 1T-201 attached to an AMP, 1T-202 attached to an AMP, 1T-203 attached to an AMP, 1T-207 attached to an AMP, 1T-208 attached to an AMP, 1T-215 attached to an AMP, 1T-216 attached to an AMP, 1T-217 attached to an AMP, 1T-218 attached to an AMP, 1T-219 attached to an AMP, 1T-221 attached to an AMP, 1T-222 attached to an AMP, 1T-223 attached to an AMP, 1T-225 attached to an AMP, 1T-226 attached to an AMP, 1T-228 attached to an AMP, 1T-230 attached to an AMP, 1T-233 attached to an AMP, 1T-234 attached to an AMP, 1T-237 attached to an AMP, 1T-238 attached to an AMP, 1T-239 attached to an AMP, 1T-240 attached to an AMP, 1T-242 attached to an AMP, 1T-243 attached to an AMP, 1T-248 attached to an AMP, 1T-249 attached to an AMP, 1T-250 attached to an AMP, 1T-252 attached to an AMP, 1T-253 attached to an AMP, 1T-256 attached to an AMP, 1T-257 attached to an AMP, 1T-258 attached to an AMP, 1T-259 attached to an AMP, 1T-260 attached to an AMP, 1T-261 attached to an AMP, 1T-262 attached to an AMP, 1T-263 attached to an AMP, 1T-264 attached to an AMP, 1T-265 attached to an AMP, 1T-266 attached to an AMP, 1T-267 attached to an AMP, 1T-268 attached to an AMP, 1T-269 attached to an AMP, 1T-270 attached to an AMP, 1T-271 attached to an AMP, 1T-272 attached to an AMP, 1T-273 attached to an AMP, 1T-274 attached to an AMP, 1T-275 attached to an AMP, 1T-276 attached to an AMP, 1T-277 attached to an AMP, 1T-278 attached to an AMP, 1T-279 attached to an AMP, 1T-280 attached to an AMP, 1T-281 attached to an AMP, 1T-282 attached to an AMP, 1T-283 attached to an AMP, 1T-284 attached to an AMP, 1T-285 attached to an AMP, 1T-286 attached to an AMP, 1T-287 attached to an AMP, 1T-288 attached to an AMP, 1T-289 attached to an AMP, 1T-290 attached to an AMP, 1T-291 attached to an AMP, 1T-292 attached to an AMP, 1T-293 attached to an AMP, 1T-294 attached to an AMP, 1T-295 attached to an AMP, 1T-296 attached to an AMP, 1T-297 attached to an AMP, 1T-298 attached to an AMP, 1T-299 attached to an AMP, 1T-300 attached to an AMP, 1T-301 attached to an AMP, 1T-302 attached to an AMP, 1T-303 attached to an AMP, 1T-304 attached to an AMP, TT-305 attached to an AMP, TT-306 attached to an AMP, TT-307 attached to an AMP, TT-308 attached to an AMP, TT-309 attached to an AMP, TT-310 attached to an AMP, TT-311 attached to an AMP, PF-060 attached to an AMP, PF-024 attached to an AMP, PF-636 attached to an AMP, PF-178 attached to an AMP, PF-761 attached to an AMP, PF-770 attached to an AMP, 1T-65 attached to an AMP, PF-141 attached to an AMP, PF-543 attached to an AMP, PF-634 attached to an AMP, PF-040 attached to an AMP, PF-051 attached to an AMP, PF-580 attached to an AMP, PF-583 attached to an AMP, 1T-36 attached to an AMP, PF-206 attached to an AMP, 1T-13 attached to an AMP, 1T-21 attached to an AMP, PF-030 attached to an AMP, PF-463 attached to an AMP, PF-380 attached to an AMP, PF-515 attached to an AMP, PF-458 attached to an AMP, PF-S018 attached to an AMP, 1T-16 attached to an AMP, PF-211 attached to an AMP, PF-002 attached to an AMP, PF-S003 attached to an AMP, PF-021 attached to an AMP, 1T-14 attached to an AMP, 1T-15 attached to an AMP, PF-629 attached to an AMP, PF-617 attached to an AMP, PF-621 attached to an AMP, PF-631 attached to an AMP, PF-C009 attached to an AMP, 1T-17 attached to an AMP, 1T-20 attached to an AMP, 1T-68 attached to an AMP, 1T-70 attached to an AMP, PF-167 attached to an AMP, PF-168 attached to an AMP, PF-170 attached to an AMP, PF-176 attached to an AMP, attached to an AMP, Rv2369c attached to an AMP, PF2-038 attached to an AMP, Rv2561 attached to an AMP, PF2-040 attached to an AMP, Rv1535 attached to an AMP, PF2-033 attached to an AMP, Rv2660c attached to an AMP, PF2-044 attached to an AMP, Rv3760 attached to an AMP, PF2-059 attached to an AMP, PF-S024 attached to an AMP, PF-001 attached to an AMP, PF-002 attached to an AMP, PF-003 attached to an AMP, PF-004 attached to an AMP, PF-005 attached to an AMP, PF-006 attached to an AMP, PF-007 attached to an AMP, PF-008 attached to an AMP, PF-009 attached to an AMP, PF-010 attached to an AMP, PF-011 attached to an AMP, PF-012 attached to an AMP, PF-013 attached to an AMP, PF-014 attached to an AMP, PF-015 attached to an AMP, PF-016 attached to an AMP, PF-017 attached to an AMP, PF-018 attached to an AMP, PF-020 attached to an AMP, PF-021 attached to an AMP, PF-022 attached to an AMP, PF-023 attached to an AMP, PF-024 attached to an AMP, PF-025 attached to an AMP, PF-026 attached to an AMP, PF-027 attached to an AMP, PF-028 attached to an AMP, PF-029 attached to an AMP, PF-030 attached to an AMP, PF-031 attached to an AMP, PF-033 attached to an AMP, PF-034 attached to an AMP, PF-035 attached to an AMP, PF-036 attached to an AMP, PF-037 attached to an AMP, PF-038 attached to an AMP, PF-039 attached to an AMP, PF-040 attached to an AMP, PF-041 attached to an AMP, PF-042 attached to an AMP, PF-043 attached to an AMP, PF-044 attached to an AMP, PF-045 attached to an AMP, PF-046 attached to an AMP, PF-047 attached to an AMP, PF-048 attached to an AMP, PF-049 attached to an AMP, PF-050 attached to an AMP, PF-051 attached to an AMP, PF-052 attached to an AMP, PF-053 attached to an AMP, PF-054 attached to an AMP, PF-055 attached to an AMP, PF-056 attached to an AMP, PF-057 attached to an AMP, PF-058 attached to an AMP, PF-060 attached to an AMP, PF-061 attached to an AMP, PF-062 attached to an AMP, PF-063 attached to an AMP, PF-064 attached to an AMP, PF-065 attached to an AMP, PF-066 attached to an AMP, PF-067 attached to an AMP, PF-068 attached to an AMP, PF-069 attached to an AMP, PF-070 attached to an AMP, PF-071 attached to an AMP, PF-073 attached to an AMP, PF-074 attached to an AMP, PF-075 attached to an AMP, PF-076 attached to an AMP, PF-097 attached to an AMP, PF-099 attached to an AMP, PF-101 attached to an AMP, PF-104 attached to an AMP, PF-123 attached to an AMP, PF-124 attached to an AMP, PF-125 attached to an AMP, PF-126 attached to an AMP, PF-127 attached to an AMP, PF-128 attached to an AMP, PF-129 attached to an AMP, PF-130 attached to an AMP, PF-131 attached to an AMP, PF-132 attached to an AMP, PF-133 attached to an AMP, PF-134 attached to an AMP, PF-135 attached to an AMP, PF-136 attached to an AMP, PF-137 attached to an AMP, PF-138 attached to an AMP, PF-139 attached to an AMP, PF-140 attached to an AMP, PF-141 attached to an AMP, PF-142 attached to an AMP, PF-143 attached to an AMP, PF-144 attached to an AMP, PF-145 attached to an AMP, PF-146 attached to an AMP, PF-147 attached to an AMP, PF-148 attached to an AMP, PF-149 attached to an AMP, PF-150 attached to an AMP, PF-151 attached to an AMP, PF-152 attached to an AMP, PF-153 attached to an AMP, PF-154 attached to an AMP, PF-155 attached to an AMP, PF-156 attached to an AMP, PF-157 attached to an AMP, PF-158 attached to an AMP, PF-159 attached to an AMP, PF-160 attached to an AMP, PF-161 attached to an AMP, PF-162 attached to an AMP, PF-163 attached to an AMP, PF-164 attached to an AMP, PF-165 attached to an AMP, PF-166 attached to an AMP, PF-167 attached to an AMP, PF-168 attached to an AMP, PF-169 attached to an AMP, PF-170 attached to an AMP, PF-171 attached to an AMP, PF-172 attached to an AMP, PF-173 attached to an AMP, PF-174 attached to an AMP, PF-175 attached to an AMP, PF-176 attached to an AMP, PF-177 attached to an AMP, PF-178 attached to an AMP, PF-180 attached to an AMP, PF-181 attached to an AMP, PF-182 attached to an AMP, PF-183 attached to an AMP, PF-184 attached to an AMP, PF-185 attached to an AMP, PF-186 attached to an AMP, PF-187 attached to an AMP, PF-188 attached to an AMP, PF-189 attached to an AMP, PF-190 attached to an AMP, PF-191 attached to an AMP, PF-192 attached to an AMP, PF-193 attached to an AMP, PF-194 attached to an AMP, PF-195 attached to an AMP, PF-196 attached to an AMP, PF-197 attached to an AMP, PF-200 attached to an AMP, PF-201 attached to an AMP, PF-202 attached to an AMP, PF-203 attached to an AMP, PF-204 attached to an AMP, PF-205 attached to an AMP, PF-206 attached to an AMP, PF-207 attached to an AMP, PF-208 attached to an AMP, PF-209 attached to an AMP, PF-210 attached to an AMP, PF-211 attached to an AMP, PF-212 attached to an AMP, PF-213 attached to an AMP, PF-214 attached to an AMP, PF-215 attached to an AMP, PF-216 attached to an AMP, PF-217 attached to an AMP, PF-218 attached to an AMP, PF-219 attached to an AMP, PF-221 attached to an AMP, PF-222 attached to an AMP, PF-223 attached to an AMP, PF-224 attached to an AMP, PF-225 attached to an AMP, PF-226 attached to an AMP, PF-228 attached to an AMP, PF-229 attached to an AMP, PF-230 attached to an AMP, PF-231 attached to an AMP, PF-232 attached to an AMP, PF-233 attached to an AMP, PF-234 attached to an AMP, PF-235 attached to an AMP, PF-236 attached to an AMP, PF-237 attached to an AMP, PF-238 attached to an AMP, PF-239 attached to an AMP, PF-240 attached to an AMP, PF-242 attached to an AMP, PF-244 attached to an AMP, PF-245 attached to an AMP, PF-246 attached to an AMP, PF-247 attached to an AMP, PF-248 attached to an AMP, PF-249 attached to an AMP, PF-252 attached to an AMP, PF-253 attached to an AMP, PF-254 attached to an AMP, PF-255 attached to an AMP, PF-256 attached to an AMP, PF-257 attached to an AMP, PF-259 attached to an AMP, PF-262 attached to an AMP, PF-263 attached to an AMP, PF-265 attached to an AMP, PF-266 attached to an AMP, PF-267 attached to an AMP, PF-268 attached to an AMP, PF-270 attached to an AMP, PF-271 attached to an AMP, PF-273 attached to an AMP, PF-275 attached to an AMP, PF-276 attached to an AMP, PF-278 attached to an AMP, PF-279 attached to an AMP, PF-281 attached to an AMP, PF-282 attached to an AMP, PF-283 attached to an AMP, PF-284 attached to an AMP, PF-285 attached to an AMP, PF-288 attached to an AMP, PF-289 attached to an AMP, PF-291 attached to an AMP, PF-292 attached to an AMP, PF-294 attached to an AMP, PF-295 attached to an AMP, PF-297 attached to an AMP, PF-299 attached to an AMP, PF-300 attached to an AMP, PF-301 attached to an AMP, PF-304 attached to an AMP, PF-305 attached to an AMP, PF-306 attached to an AMP, PF-307 attached to an AMP, PF-308 attached to an AMP, PF-309 attached to an AMP, PF-311 attached to an AMP, PF-313 attached to an AMP, PF-314 attached to an AMP, PF-317 attached to an AMP, PF-319 attached to an AMP, PF-320 attached to an AMP, PF-323 attached to an AMP, PF-324 attached to an AMP, PF-325 attached to an AMP, PF-326 attached to an AMP, PF-328 attached to an AMP, PF-329 attached to an AMP, PF-333 attached to an AMP, PF-334 attached to an AMP, PF-336 attached to an AMP, PF-337 attached to an AMP, PF-338 attached to an AMP, PF-340 attached to an AMP, PF-344 attached to an AMP, PF-347 attached to an AMP, PF-348 attached to an AMP, PF-349 attached to an AMP, PF-350 attached to an AMP, PF-351 attached to an AMP, PF-352 attached to an AMP, PF-353 attached to an AMP, PF-354 attached to an AMP, PF-355 attached to an AMP, PF-356 attached to an AMP, PF-357 attached to an AMP, PF-358 attached to an AMP, PF-359 attached to an AMP, PF-360 attached to an AMP, PF-361 attached to an AMP, PF-362 attached to an AMP, PF-363 attached to an AMP, PF-364 attached to an AMP, PF-365 attached to an AMP, PF-366 attached to an AMP, PF-367 attached to an AMP, PF-368 attached to an AMP, PF-369 attached to an AMP, PF-370 attached to an AMP, PF-371 attached to an AMP, PF-372 attached to an AMP, PF-373 attached to an AMP, PF-374 attached to an AMP, PF-375 attached to an AMP, PF-376 attached to an AMP, PF-378 attached to an AMP, PF-379 attached to an AMP, PF-380 attached to an AMP, PF-381 attached to an AMP, PF-382 attached to an AMP, PF-383 attached to an AMP, PF-384 attached to an AMP, PF-385 attached to an AMP, PF-386 attached to an AMP, PF-387 attached to an AMP, PF-388 attached to an AMP, PF-389 attached to an AMP, PF-390 attached to an AMP, PF-392 attached to an AMP, PF-393 attached to an AMP, PF-394 attached to an AMP, PF-395 attached to an AMP, PF-396 attached to an AMP, PF-397 attached to an AMP, PF-398 attached to an AMP, PF-399 attached to an AMP, PF-400 attached to an AMP, PF-401 attached to an AMP, PF-402 attached to an AMP, PF-403 attached to an AMP, PF-404 attached to an AMP, PF-405 attached to an AMP, PF-406 attached to an AMP, PF-407 attached to an AMP, PF-408 attached to an AMP, PF-409 attached to an AMP, PF-410 attached to an AMP, PF-411 attached to an AMP, PF-412 attached to an AMP, PF-413 attached to an AMP, PF-414 attached to an AMP, PF-416 attached to an AMP, PF-417 attached to an AMP, PF-418 attached to an AMP, PF-421 attached to an AMP, PF-422 attached to an AMP, PF-423 attached to an AMP, PF-424 attached to an AMP, PF-425 attached to an AMP, PF-426 attached to an AMP, PF-427 attached to an AMP, PF-428 attached to an AMP, PF-429 attached to an AMP, PF-430 attached to an AMP, PF-431 attached to an AMP, PF-432 attached to an AMP, PF-433 attached to an AMP, PF-434 attached to an AMP, PF-435 attached to an AMP, PF-436 attached to an AMP, PF-438 attached to an AMP, PF-439 attached to an AMP, PF-440 attached to an AMP, PF-441 attached to an AMP, PF-442 attached to an AMP, PF-443 attached to an AMP, PF-444 attached to an AMP, PF-445 attached to an AMP, PF-446 attached to an AMP, PF-447 attached to an AMP, PF-448 attached to an AMP, PF-450 attached to an AMP, PF-451 attached to an AMP, PF-452 attached to an AMP, PF-453 attached to an AMP, PF-454 attached to an AMP, PF-456 attached to an AMP, PF-457 attached to an AMP, PF-458 attached to an AMP, PF-459 attached to an AMP, PF-460 attached to an AMP, PF-461 attached to an AMP, PF-462 attached to an AMP, PF-463 attached to an AMP, PF-464 attached to an AMP, PF-465 attached to an AMP, PF-466 attached to an AMP, PF-467 attached to an AMP, PF-469 attached to an AMP, PF-470 attached to an AMP, PF-471 attached to an AMP, PF-472 attached to an AMP, PF-473 attached to an AMP, PF-474 attached to an AMP, PF-475 attached to an AMP, PF-476 attached to an AMP, PF-477 attached to an AMP, PF-478 attached to an AMP, PF-479 attached to an AMP, PF-480 attached to an AMP, PF-481 attached to an AMP, PF-482 attached to an AMP, PF-483 attached to an AMP, PF-484 attached to an AMP, PF-486 attached to an AMP, PF-487 attached to an AMP, PF-488 attached to an AMP, PF-489 attached to an AMP, PF-490 attached to an AMP, PF-491 attached to an AMP, PF-492 attached to an AMP, PF-493 attached to an AMP, PF-494 attached to an AMP, PF-495 attached to an AMP, PF-496 attached to an AMP, PF-497 attached to an AMP, PF-498 attached to an AMP, PF-499 attached to an AMP, PF-500 attached to an AMP, PF-501 attached to an AMP, PF-502 attached to an AMP, PF-503 attached to an AMP, PF-504 attached to an AMP, PF-505 attached to an AMP, PF-506 attached to an AMP, PF-507 attached to an AMP, PF-508 attached to an AMP, PF-509 attached to an AMP, PF-510 attached to an AMP, PF-511 attached to an AMP, PF-512 attached to an AMP, PF-513 attached to an AMP, PF-514 attached to an AMP, PF-515 attached to an AMP, PF-517 attached to an AMP, PF-518 attached to an AMP, PF-519 attached to an AMP, PF-520 attached to an AMP, PF-521 attached to an AMP, PF-522 attached to an AMP, PF-523 attached to an AMP, PF-524 attached to an AMP, PF-526 attached to an AMP, PF-527 attached to an AMP, PF-528 attached to an AMP, PF-529 attached to an AMP, PF-537 attached to an AMP, PF-539 attached to an AMP, PF-540 attached to an AMP, PF-542 attached to an AMP, PF-543 attached to an AMP, PF-544 attached to an AMP, PF-545 attached to an AMP, PF-546 attached to an AMP, PF-547 attached to an AMP, PF-548 attached to an AMP, PF-549 attached to an AMP, PF-550 attached to an AMP, PF-551 attached to an AMP, PF-552 attached to an AMP, PF-553 attached to an AMP, PF-554 attached to an AMP, PF-555 attached to an AMP, PF-556 attached to an AMP, PF-557 attached to an AMP, PF-558 attached to an AMP, PF-559 attached to an AMP, PF-560 attached to an AMP, PF-562 attached to an AMP, PF-563 attached to an AMP, PF-564 attached to an AMP, PF-566 attached to an AMP, PF-567 attached to an AMP, PF-568 attached to an AMP, PF-569 attached to an AMP, PF-570 attached to an AMP, PF-572 attached to an AMP, PF-573 attached to an AMP, PF-575 attached to an AMP, PF-576 attached to an AMP, PF-577 attached to an AMP, PF-578 attached to an AMP, PF-579 attached to an AMP, PF-580 attached to an AMP, PF-581 attached to an AMP, PF-583 attached to an AMP, PF-584 attached to an AMP, PF-585 attached to an AMP, PF-586 attached to an AMP, PF-587 attached to an AMP, PF-588 attached to an AMP, PF-589 attached to an AMP, PF-590 attached to an AMP, PF-592 attached to an AMP, PF-593 attached to an AMP, PF-594 attached to an AMP, PF-595 attached to an AMP, PF-596 attached to an AMP, PF-597 attached to an AMP, PF-598 attached to an AMP, PF-599 attached to an AMP, PF-600 attached to an AMP, PF-601 attached to an AMP, PF-602 attached to an AMP, PF-603 attached to an AMP, PF-604 attached to an AMP, PF-605 attached to an AMP, PF-607 attached to an AMP, PF-609 attached to an AMP, PF-610 attached to an AMP, PF-612 attached to an AMP, PF-613 attached to an AMP, PF-614 attached to an AMP, PF-615 attached to an AMP, PF-616 attached to an AMP, PF-617 attached to an AMP, PF-619 attached to an AMP, PF-621 attached to an AMP, PF-622 attached to an AMP, PF-623 attached to an AMP, PF-625 attached to an AMP, PF-626 attached to an AMP, PF-627 attached to an AMP, PF-629 attached to an AMP, PF-630 attached to an AMP, PF-631 attached to an AMP, PF-632 attached to an AMP, PF-634 attached to an AMP, PF-635 attached to an AMP, PF-636 attached to an AMP, PF-637 attached to an AMP, PF-638 attached to an AMP, PF-639 attached to an AMP, PF-640 attached to an AMP, PF-641 attached to an AMP, PF-642 attached to an AMP, PF-643 attached to an AMP, PF-644 attached to an AMP, PF-645 attached to an AMP, PF-646 attached to an AMP, PF-647 attached to an AMP, PF-648 attached to an AMP, PF-649 attached to an AMP, PF-650 attached to an AMP, PF-651 attached to an AMP, PF-652 attached to an AMP, PF-653 attached to an AMP, PF-654 attached to an AMP, PF-655 attached to an AMP, PF-656 attached to an AMP, PF-657 attached to an AMP, PF-658 attached to an AMP, PF-659 attached to an AMP, PF-660 attached to an AMP, PF-661 attached to an AMP, PF-662 attached to an AMP, PF-663 attached to an AMP, PF-664 attached to an AMP, PF-665 attached to an AMP, PF-666 attached to an AMP, PF-667 attached to an AMP, PF-668 attached to an AMP, PF-669 attached to an AMP, PF670 attached to an AMP, PF-671 attached to an AMP, PF-673 attached to an AMP, PF-674 attached to an AMP, PF-675 attached to an AMP, PF-676 attached to an AMP, PF-677 attached to an AMP, PF-678 attached to an AMP, PF-679 attached to an AMP, PF-680 attached to an AMP, PF-681 attached to an AMP, PF-682 attached to an AMP, PF-683 attached to an AMP, PF-684 attached to an AMP, PF-685 attached to an AMP, PF-686 attached to an AMP, PF-687 attached to an AMP, PF-688 attached to an AMP, PF-689 attached to an AMP, PF-690 attached to an AMP, PF-691 attached to an AMP, PF-692 attached to an AMP, PF-693 attached to an AMP, PF-694 attached to an AMP, PF-695 attached to an AMP, PF-696 attached to an AMP, PF-697 attached to an AMP, PF-698 attached to an AMP, PF-699 attached to an AMP, PF-700 attached to an AMP, PF-701 attached to an AMP, PF-702 attached to an AMP, PF-703 attached to an AMP, PF-704 attached to an AMP, PF-705 attached to an AMP, PF-706 attached to an AMP, PF-707 attached to an AMP, PF-709 attached to an AMP, PF-711 attached to an AMP, PF-712 attached to an AMP, PF-714 attached to an AMP, PF-716 attached to an AMP, PF-717 attached to an AMP, PF-720 attached to an AMP, PF-724 attached to an AMP, PF-727 attached to an AMP, PF-728 attached to an AMP, PF-731 attached to an AMP, PF-732 attached to an AMP, PF-733 attached to an AMP, PF-735 attached to an AMP, PF-737 attached to an AMP, PF-738 attached to an AMP, PF-741 attached to an AMP, PF-744 attached to an AMP, PF-745 attached to an AMP, PF-746 attached to an AMP, PF-748 attached to an AMP, PF-749 attached to an AMP, PF-750 attached to an AMP, PF-751 attached to an AMP, PF-752 attached to an AMP, PF-756 attached to an AMP, PF-757 attached to an AMP, PF-758 attached to an AMP, PF-760 attached to an AMP, PF-761 attached to an AMP, PF-762 attached to an AMP, PF-763 attached to an AMP, PF-764 attached to an AMP, PF-765 attached to an AMP, PF-766 attached to an AMP, PF-767 attached to an AMP, PF-768 attached to an AMP, PF-770 attached to an AMP, PF-772 attached to an AMP, PF-773 attached to an AMP, PF-776 attached to an AMP, PF-777 attached to an AMP, PF-778 attached to an AMP, PF-779 attached to an AMP, PF-780 attached to an AMP, PF-781 attached to an AMP, PF-782 attached to an AMP, PF-783 attached to an AMP, PF-784 attached to an AMP, PF-785 attached to an AMP, PF-786 attached to an AMP, PF-787 attached to an AMP, PF-788 attached to an AMP, PF-789 attached to an AMP, PF-790 attached to an AMP, PF-791 attached to an AMP, PF-792 attached to an AMP, PF-793 attached to an AMP, PF-794 attached to an AMP, PF-795 attached to an AMP, PF-796 attached to an AMP, PF-797 attached to an AMP, PF-798 attached to an AMP, PF-799 attached to an AMP, PF-800 attached to an AMP, PF-C003 attached to an AMP, PF-C008 attached to an AMP, PF-C034 attached to an AMP, PF-C045 attached to an AMP, PF-C050 attached to an AMP, PF-C052 attached to an AMP, PF-C055 attached to an AMP, PF-C057 attached to an AMP, PF-C058 attached to an AMP, PF-C061 attached to an AMP, PF-C062 attached to an AMP, PF-C063 attached to an AMP, PF-C064 attached to an AMP, PF-C065 attached to an AMP, PF-C068 attached to an AMP, PF-C069 attached to an AMP, PF-C071 attached to an AMP, PF-C072 attached to an AMP, PF-C075 attached to an AMP, PF-C0080 attached to an AMP, PF-C0084 attached to an AMP, PF-C0085 attached to an AMP, PF-C0086 attached to an AMP, PF-C088 attached to an AMP, PF-C091 attached to an AMP, PF-C093 attached to an AMP, PF-C095 attached to an AMP, PF-C098 attached to an AMP, PF-C120 attached to an AMP, PF-C131 attached to an AMP, PF-C134 attached to an AMP, PF-C 135 attached to an AMP, PF-C 136 attached to an AMP, PF-C 137 attached to an AMP, PF-C 139 attached to an AMP, PF-C 142 attached to an AMP, PF-C 143 attached to an AMP, PF-C145 attached to an AMP, PF-C160 attached to an AMP, PF-C180 attached to an AMP, PF-C181 attached to an AMP, PF-C194 attached to an AMP, PF-C259 attached to an AMP, PF-C271 attached to an AMP, PF-C273 attached to an AMP, PF-C281 attached to an AMP, PF-C285 attached to an AMP, PF-C290 attached to an AMP, PF-C291 attached to an AMP, PF-S003 attached to an AMP, PF-S004 attached to an AMP, PF-S007 attached to an AMP, PF-S015 attached to an AMP, PF-S018 attached to an AMP, PF-S023 attached to an AMP, PF-S026 attached to an AMP, or PF-S029 attached to an AMP. In certain illustrative embodiments the STAMP comprises any one of the foregoing targeting peptides attached (directly or through a linker (e.g., one of the linkers shown in Table 5)) to an one of the AMPs shown in Table 4 (e.g., one of K-1, K-2, K-7, K-8, K-9, K-10, K-11, K-12, K-13, K-14, K-15, K-16, K-17, K-18, K-19, K-20, K-21, K-22, 1T-88, PF-531, PF-527, PF-672, PF-606, PF-547, PF-C06, PF-545, PF-278, PF-283, PF-307, PF-168, PF-538, PF-448, PF-583, PF-600, PF-525, PF-529, PF-148, PF-530, PF-522, PF-497, PF-499, PF-322, PF-511, PF-512, PF-520, PF-521, PF-523, PF-524, PF-209, PF-437, V, W, X, Y, or Z).

In certain embodiments in any of the foregoing methods, diagnostic devices, or diagnostic test units the selective permeabilization reagent comprises one of the antimicrobial peptides selected from Table 4 attached to a targeting peptide forming a STAMP. In certain embodiments the targeting peptide is a peptide found in Table 2. Thus in various embodiments, the permeabilization reagent comprises a STAMP such as K-1 attached to one of the peptides 1T-3 through PF-Z in Table 2, K-2 attached to one of the peptides 1T-3 through PF-Z in Table 2, K-7 attached to one of the peptides 1T-3 through PF-Z in Table 2, K-8 attached to one of the peptides 1T-3 through PF-Z in Table 2, K-9 attached to one of the peptides 1T-3 through PF-Z in Table 2, K-10 attached to one of the peptides 1T-3 through PF-Z in Table 2, K-11 attached to one of the peptides 1T-3 through PF-Z in Table 2, K-12 attached to one of the peptides 1T-3 through PF-Z in Table 2, K-13 attached to one of the peptides 1T-3 through PF-Z in Table 2, K-14 attached to one of the peptides 1T-3 through PF-Z in Table 2, K-15 attached to one of the peptides 1T-3 through PF-Z in Table 2, K-16 attached to one of the peptides 1T-3 through PF-Z in Table 2, K-17 attached to one of the peptides 1T-3 through PF-Z in Table 2, K-18 attached to one of the peptides 1T-3 through PF-Z in Table 2, K-19 attached to one of the peptides 1T-3 through PF-Z in Table 2, K-20 attached to one of the peptides 1T-3 through PF-Z in Table 2, K-21 attached to one of the peptides 1T-3 through PF-Z in Table 2, K-22 attached to one of the peptides 1T-3 through PF-Z in Table 2, 1T-88 attached to one of the peptides 1T-3 through PF-Z in Table 2, PF-531 attached to one of the peptides 1T-3 through PF-Z in Table 2, PF-527 attached to one of the peptides 1T-3 through PF-Z in Table 2, PF-672 attached to one of the peptides 1T-3 through PF-Z in Table 2, PF-606 attached to one of the peptides 1T-3 through PF-Z in Table 2, PF-547 attached to one of the peptides 1T-3 through PF-Z in Table 2, PF-606 attached to one of the peptides 1T-3 through PF-Z in Table 2, PF-545 attached to one of the peptides 1T-3 through PF-Z in Table 2, PF-278 attached to one of the peptides 1T-3 through PF-Z in Table 2, PF-283 attached to one of the peptides 1T-3 through PF-Z in Table 2, PF-307 attached to one of the peptides 1T-3 through PF-Z in Table 2, PF-168 attached to one of the peptides 1T-3 through PF-Z in Table 2, PF-538 attached to one of the peptides 1T-3 through PF-Z in Table 2, PF-448 attached to one of the peptides 1T-3 through PF-Z in Table 2, PF-583 attached to one of the peptides 1T-3 through PF-Z in Table 2, PF-600 attached to one of the peptides 1T-3 through PF-Z in Table 2, PF-525 attached to one of the peptides 1T-3 through PF-Z in Table 2, PF-529 attached to one of the peptides 1T-3 through PF-Z in Table 2, PF-148 attached to one of the peptides 1T-3 through PF-Z in Table 2, PF-530 attached to one of the peptides 1T-3 through PF-Z in Table 2, PF-522 attached to one of the peptides 1T-3 through PF-Z in Table 2, PF-497 attached to one of the peptides 1T-3 through PF-Z in Table 2, PF-499 attached to one of the peptides 1T-3 through PF-Z in Table 2, PF-322 attached to one of the peptides 1T-3 through PF-Z in Table 2, PF-511 attached to one of the peptides 1T-3 through PF-Z in Table 2, PF-512 attached to one of the peptides 1T-3 through PF-Z in Table 2, PF-520 attached to one of the peptides 1T-3 through PF-Z in Table 2, PF-521 attached to one of the peptides 1T-3 through PF-Z in Table 2, PF-523 attached to one of the peptides 1T-3 through PF-Z in Table 2, PF-524 attached to one of the peptides 1T-3 through PF-Z in Table 2, PF-209 attached to one of the peptides 1T-3 through PF-Z in Table 2, PF-437 attached to one of the peptides 1T-3 through PF-Z in Table 2, V attached to one of the peptides 1T-3 through PF-Z in Table 2, W attached to one of the peptides 1T-3 through PF-Z in Table 2, X attached to one of the peptides 1T-3 through PF-Z in Table 2, Y attached to one of the peptides 1T-3 through PF-Z in Table 2, or Z attached to one of the peptides 1T-3 through PF-Z in Table 2.

In certain embodiments, the selective permeabilization reagent is not or does not comprise a biological organism. In certain embodiments the selective permeabilization reagent is not a bacteriophage (phage).

DEFINITIONS

The terms “selectively permeabilize” or “selectively lyse” refers to increasing the permeability of the membrane (and/or where present a cell wall) of a target microorganism (or target cell) while having no or a substantially reduced effect on other target microorganisms (or target cell(s)) that may be present in the sample. In certain embodiments, a target microorganism or cell is selectively permeabilized by a reagent when contact of the reagent permits entry of at least 1.2×, preferably at least 1.5×, or 2×, more preferably at least 3×, 5×, or 10× the amount of the reagent into the target microorganism or cell as compared to the amount of the reagent that enters other microorganisms or cells in the same sample.

A “selective permeabilization reagent” refers to a reagent that selectively permeabilizes or selectively lyses a particular target microorganism or a particular group of target microorganisms (e.g., gram−bacteria, gram+bacteria, etc.).

The term “detection reagent” refers to a reagent or combination of reagents that can be used to detect the presence or quantity of a metabolite, enzyme, ionic species or other cellular component.

An “impermeant label” refers to a label that is unable to pass through or substantially unable to pass through a semipermeable membrane (e.g., a cell membrane), and/or where present a cell wall. The impermeant label thereby distinguishes a permeabilized or lysed cell from an unaltered (intact) cell.

A sample refers to target and substance or collection of substances in which or from which it is desired to ascertain the presence and/or quantity of one or more target microorganisms and/or cells. Illustrative samples, include, but are not limited to, samples of water, soil, crops and vegetation, meats, fish, and poultry, milk and cheese, and various biological samples derived from human or non-human organisms. In certain embodiments the sample comprises isolated cells, a mixed cellular community, or a clinical sample. Clinical sample materials include, but are not limited to blood or blood fractions, cerebrospinal fluid, urine, saliva, mucus, tissue samples, and the like.

As used herein, an “antibody” refers to a protein consisting of one or more polypeptides substantially encoded by immunoglobulin genes or fragments of immunoglobulin genes. The recognized immunoglobulin genes include the kappa, lambda, alpha, gamma, delta, epsilon and mu constant region genes, as well as myriad immunoglobulin variable region genes. Light chains are classified as either kappa or lambda. Heavy chains are classified as gamma, mu, alpha, delta, or epsilon, which in turn define the immunoglobulin classes, IgG, IgM, IgA, IgD and IgE, respectively.

A typical immunoglobulin (antibody) structural unit is known to comprise a tetramer. Each tetramer is composed of two identical pairs of polypeptide chains, each pair having one “light” (about 25 kD) and one “heavy” chain (about 50-70 kD). The N-terminus of each chain defines a variable region of about 100 to 110 or more amino acids primarily responsible for antigen recognition. The terms variable light chain (V_L) and variable heavy chain (V_H) refer to these light and heavy chains respectively.

Antibodies exist as intact immunoglobulins or as a number of well characterized fragments produced by digestion with various peptidases. Thus, for example, pepsin digests an antibody below the disulfide linkages in the hinge region to produce F(ab)′₂, a dimer of Fab which itself is a light chain joined to V_H-C_H1 by a disulfide bond. The F(ab)′₂may be reduced under mild conditions to break the disulfide linkage in the hinge region thereby converting the (Fab′)₂dimer into an Fab′ monomer. The Fab′ monomer is essentially an Fab with part of the hinge region (see, Fundamental Immunology, W. E. Paul, ed., Raven Press, N.Y. (1993), for a more detailed description of other antibody fragments). While various antibody fragments are defined in terms of the digestion of an intact antibody, one of skill will appreciate that such Fab′ fragments may be synthesized de novo either chemically or by utilizing recombinant DNA methodology. Thus, the term antibody, as used herein also includes antibody fragments either produced by the modification of whole antibodies or synthesized de novo using recombinant DNA methodologies, including, but are not limited to, Fab′₂, IgG, IgM, IgA, scFv, dAb, nanobodies, unibodies, and diabodies.

In certain embodiments antibodies and fragments of the present invention can be bispecific. Bispecific antibodies or fragments can be of several configurations. For example, bispecific antibodies may resemble single antibodies (or antibody fragments) but have two different antigen binding sites (variable regions). In various embodiments bispecific antibodies can be produced by chemical techniques (Kranz et al. (1981) Proc. Natl. Acad. Sci., USA, 78: 5807), by “polydoma” techniques (see, e.g., U.S. Pat. No. 4,474,893), or by recombinant DNA techniques. In certain embodiments bispecific antibodies of the present invention can have binding specificities for at least two different epitopes, at least one of which is an epitope of a microbial organism. The microbial binding antibodies and fragments can also be heteroantibodies. Heteroantibodies are two or more antibodies, or antibody binding fragments (e.g., Fab) linked together, each antibody or fragment having a different specificity.

The term “STAMP” refers to Specifically Targeted Anti-Microbial Peptides. In various embodiments, a STAMP comprises one or more peptide targeting moieties attached to one or more antimicrobial moieties (e.g., antimicrobial peptides (AMPs)). An MH-STAMP is a STAMP bearing two or more targeting domains (i.e., a multi-headed STAMP).

BRIEF DESCRIPTION OF THE DRAWINGS

FIGS. 1A and 1B schematically illustrate two embodiments of the methods described herein. FIG. 1A illustrates that in contrast to a typical lysis reagent (e.g., a detergent) a selectively permeabilization reagent permeabilizes the target microorganism (e.g., S. mutans) without substantially permeabilizing other microorganisms in the sample. This permits metabolites, enzymes, or other cellular components to exit the microorganism where they are detected indicating the presence and/or the amount of the target microorganism. FIG. 1B illustrates an assay where the selective permeabilization reagent permits entry of an impermeant label into the target microorganism

FIG. 2 illustrates one method of performing an assay described herein. As illustrated therein, in step A, a sample (e.g., saliva) is collected on a collection device (e.g., a swab). In step B, the sample is incubated with a selective permeabilization reagent (e.g., a STAMP). In step C, a detection reagent is added to the mix and the reaction is optionally agitated. In step D, the reaction mixture is inserted into a test reader, and in step E, the results are read.

FIG. 3 shows that assays described herein are capable of quantitatively detecting as little as 10⁴cells/ml of cultured S. mutans grown in the lab.

FIG. 4 shows that assays described herein are capable of quantifying S. mutans spiked in a fresh unfiltered saliva sample.

FIG. 5 demonstrates targeted permeabilization of spiked S. mutans in fresh saliva samples.

FIG. 6 shows a schematic diagram of one illustrative diagnostic test unit.

DETAILED DESCRIPTION

Novel methods and devices for the detection and/or quantification of microorganisms are provided herein. The methods are rapid, do not require significant instrumentation, and show high specificity and selectivity.

In certain embodiments the methods involve contacting a target microorganism (or a sample containing one or more target microorganisms) with a permeabilization reagent that selectively permeabilizes or lyses the target microorganism. The selective permeabilization releases enzymes or metabolites from the target microorganism where they can be contacted with one or more detection reagents that produce signal(s) upon contact/reaction with the enzyme or metabolite (see, e.g., FIG. 1). The magnitude of the signal provides an indication of the presence and/or amount of target microorganism present. Because the permeabilization reagent is selective for the target microorganism, the assay provides a signal that predominantly represents the presence or quantity of the target microorganism even in the presence of other microorganisms.

It will be appreciated, that in certain embodiments, the selective permeabilization permits entrance of the detection reagent(s) into the target microorganism where they react with metabolites or substrates and the reaction provides an indication of the presence and/or amount of the target microorganism.

Similarly, in another illustrative embodiment, the methods involve contacting the target microorganism (e.g., in a biological sample) with a permeabilization reagent that selectively permeabilizes the microorganism. The microorganism is contacted with a cell-impermeant label (e.g., a cell impermeant fluorescent dye (e.g., propidium iodide, SYTOX Green, etc.), a colorimetric dye (e.g. Trypan blue, etc.)) and, because the microorganisms is selectively permeabilized by the permeabilization reagent, the label enters the microorganism. Because the permeabilization reagent is selective for the target microorganism, other microorganisms that may be present are not permeabilized and internalize little or no label (see, e.g., FIG. 1B). The label is then detected in the microorganism where the presence or amount of said label associated with the microorganism indicates the presence or amount of the target microorganism in said sample. Additionally, a concentration step (filter, centrifugation, other) allows increased sensitivity of the method.

In certain embodiments microorganisms/cells permeabilized and stained with fluorescent or colorimetric dyes can be filtered (single pore size filter, serial filters, etc.) to remove debris, concentrate and capture bacteria/cells on the filter surface. Bacteria/cells can be quantitated by measuring the fluorescent or color intensity using a measuring device or by visual observation. Additionally bacteria/cells captured on the filter surface can be imaged via microscopy, solid-phase cytometry or other method.

The methods described herein can be performed to detect essentially any microorganism. Such microorganisms include, but are not limited to bacteria, yeasts, fungi, molds, viruses, algae, protozoa, and the like. In certain embodiments, the methods can be used to detect and/or quantify Gram-negative bacteria (e.g., Acinetobacter baumannii, Escherichia coli, Fusobacterium nucleatum, Pseudomonas aeruginosa, Porphyromonas gingivalis, and the like), Gram-positive bacteria (e.g., Actinomyces naeslundii, Bacillus subtilis, Clostridium difficile, Enterococcus faecalis, Staphylococcus aureus (and MRSA), S. epidermidis, Streptococcus mutans, Streptococcus pneumoniae, and the like), yeast or fungi (e.g., Aspergillus niger, Candida albicans, Malassezia furfur, Trichophyton rubrum, and the like) (see, e.g., Table 1 identifying illustrative target microorganisms and associated pathologies).

TABLE 1

Illustrative target microorganisms and associated pathology.

Acinetobacter
baumannii

Pathogenic gram-negative bacillus that is naturally sensitive

(A.baumannii)
to relatively few antibiotics.

Actinomyces
naeslundii

Gram positive rod shaped bacteria that occupy the oral

(A.naeslundii)
cavity and are implicated in periodontal disease and root caries.

Aspergillus
niger

A fungal infection that often causes a black mold to appear

(A.niger)
on some fruit and vegetables but may also infect humans

through inhalation of fungal spores.

Bacteroides
fragilis

Gram positive bacilli that are opportunistic human

(B.fragilis)
pathogens, causing infections of the peritoneal cavity,

gastrointestinal surgery, and appendicitis via abscess

formation, inhibiting phagocytosis. Resistant to a wide

variety of antibiotics --β-lactams, aminoglycosides, and

recently many species have acquired resistance to

erythromycin and tetracycline.

Bacillus
subtilis

Gram-positive, catalase-positive bacterium.

(B.subtilis)

Candida
albicans

Causal agent of opportunistic oral and genital fungal

(C.albicans)
infections in humans.

Clostridium
difficile

A gram-positive, anaerobic, spore-forming bacillus that is

(C.difficile)
responsible for the development of antibiotic-associated

diarrhea and colitis.

Corynebacterium
jeikeium

Gram positive, opportunistic pathogen primarily of

(C.jeikeium)
immunocompromised (neutropenic) patients. Highly

resistant to antibiotics

Campylobacter
jejuni

Gram negative cause of human gastroenteritis/food

(C.jejuni)
poisoning.

Escherichia
coli

Gram negative rod-shaped bacterium commonly found in the

(E.coli)
lower intestine of warm-blooded organisms. Certain strains

cause serious food poisoning in humans.

Enterococcus
faecalis

Gram-positive commensal bacterium

(E.faecalis)

Fusobacterium
nucleatum

Gram negative schizomycetes bacterium often seen in

(F.nucleatum)
necrotic tissue and implicated, but not conclusively, with

other organisms in the causation and perpetuation of

periodontal disease.

Lactobacillus
acidophilus

Gram-positive commensal bacterium.

(L.acidophilus)

Legionella
pneumophila

Gram negative bacterium that is the causative agent of

(L.pneumophila)
legionellosis or Legionnaires' disease.

(Micrococcusluteus)
Gram positive, spherical, saprotrophic bacterium found in

M.
luteus

soil, dust, water and air, and as part of the normal flora of the

mammalian skin. The bacterium also colonizes the human

mouth, mucosae, oropharynx and upper respiratory tract.

Considered an emerging nosocomial pathogen in

immunocompromised patients.

Mycobacterium
smegmatis

Gram-variable (acid-fast) soil-dwelling organism utilized as

(M.smegmatis)
a proxy for Mycobacterium tuberculosis during research and

development.

Malassezia
furfur

Yeast-cutaneous pathogen.

(M.furfur)

Methicillin-resistant
Any strain of Staphylococcusaureus bacteria (gram positive)

Staphylococcus
aureus

that is resistant to a one or more members of a large group of

(MRSA)
antibiotics called the beta-lactams. Responsible for skin and

systemic infections.

Myxococcus
xanthus

Gram negative cells that form biofilms and display primitive

(M.xanthus)
social motility and fruiting body organization.

Pseudomonas
aeruginosa

Gram-negative rod. Frequent opportunistic pathogen and

P.
aeruginosa

infects burn wounds. Causes ear infections in children.

Infects the lungs of cystic fibrosis patients.

Porphyromonas
gingivalis

Non-motile, gram-negative, rod-shaped, anaerobic

(P.gingivalis)
pathogenic bacterium (periodontal disease)

Proteus
mirabilis

Gram-negative, facultatively anaerobic bacterium. Causes

(P.mirabilis)
90% of all ‘Proteus’ infections in humans.

Staphylococcus
epidermidis

Gram-positive, coagulase-negative cocci. Nosocomial

(S.epidermidis)
pathogen associated with infection (biofilm) of implanted

medical device.

Streptococcus
mutans

Gram-positive, facultatively anaerobic bacterium commonly

(S.mutans)
found in the human oral cavity and is a significant

contributor to tooth decay

Streptococcus
pneumoniae

Gram-positive, alpha-hemolytic, bile soluble aerotolerant

(S.pneumoniae)
anaerobe. Causal agent for streptococcal pneumonia.

Treponema
denticola

Gram-negative oral spirochete associated with the incidence

(T.denticola)
and severity of human periodontal disease and localized

osteitis following tooth extraction (dry socket)

Trichophyton
rubrum

Most common cause of athlete's foot, jock itch and

(T.rubrum)
ringworm.

The methods described herein are not only useful to detect pathogens in biological samples derived from animals or humans, but can also be used to detect contaminants in foods/agricultural products, to detect environmental contaminants in, for example, soil or water, to detect contaminants in clean/sterile environments (e.g., hospitals, operating rooms), to detect contaminants of devices (e.g., surgical devices, etc.), and the like.

By way of example, Campylobacter jejuni is a common contaminant of poultry. Similarly, Clostridium botulinum is a common food toxin. Escherichia coli is a common toxin found in ground beef, raw milk, chicken, vegetables, and fruit. Salmonella typhimurium is typically found in meats, poultry, eggs or milk products. Shigella is often found as a contaminant of salads (potato, chicken, seafood, vegetable), raw vegetables, milk and other dairy products, and meat products especially poultry. Staphylococcus aureus is typically found in custard or cream-filled baked goods, ham, poultry, eggs, potato salad, cream sauces, sandwich fillings. Vibrio cholera, the causal agent of cholera can be transmitted by water or food. Vibrio vulnificus is a free-living ocean bacterium that can cause food borne illnesses from contaminated seafood and is especially dangerous in the warm weather months when eating shellfish that are undercooked or raw. Water contamination is usually due to the presence of three bacteria, E. coli, Clostridium perfringens, and enterococci, the bacteria normally found in the feces of people and many animals. The methods described herein can easily be used to screen foods, processing plants, and equipment for these various pathogens.

Similarly, the methods can be used to detect certain parasites. Such parasites include, but are not limited to Entamoeba histolytica, Giardia duodenalis, Cryptosporidium parvum, Cyclospora cayetanensis, Toxoplasma gondii, Trichinella spiralis, Taenia saginatajsolium, and Taenia saginata.

In certain embodiments the methods can be used to distinguish particular strains of microorganism.

The methods described herein are not limited to the detection of microorganisms. It will be recognized that such methods can be used to detect particular cells (using a targeting moiety that binds the target cell type), tissues comprising such cells, and the like.

The methods described herein are not limited to the detection/quantification of a single class (e.g., gram+/gram−), genus/species/strain of microorganism at a time. Using various formats, multiple microorganisms can be detected/quantified at a time. For example, the assays can be set up in a multi-well plate (e.g., 6, 24, 96, 384, 1536 well microtiter plates) where different wells contain different permeabilization reagents selective for different microorganisms and thereby permit detection of different target microorganisms. Similarly different permeabilization reagents can be provided in different regions of an array. In various embodiments, flow through systems can be used where different regions in a channel or tube can introduce a sample to different permeabilization reagents selective for different microorganisms and thereby permit sequential screening for different target microorganisms.

In one illustrative embodiment (schematically shown in FIG. 2), a saliva sample is collected (using for example, a swab). The saliva sample is deposited in a reaction chamber with a selective permeabilizing reagent (e.g., a STAMP) and the in another control reaction chamber (e.g., without a selective permeabilizing reagent). A luciferase reagent is added to the samples and they are mixed. Then light from the samples is measured to determine the presence of a targeted, permeabilized microorganism (e.g. S. mutans).

In a dental office, for example, the procedure could be carried out as follows: 1) Upon entering the exam room the dental assistant unpackages the S. mutans diagnostic containing a control and test reaction; 2) The patient holds the saliva collector in his mouth for 10 second allowing it to absorb saliva and bacteria present in the oral cavity; 3) The collector is removed, e.g., by the dental assistant and incubated for e.g., 10 minutes; 4) After incubation the luciferase reagent is added to the collected saliva and luminescence is measured in a handheld luminometer as prompted by the device to determine the presence and/or quantity of S. mutans.

The foregoing uses, target microorganisms, and embodiments are illustrative and not limiting. Using the teachings provided herein, other variants on the assays and uses thereof will be available to one of skill in the art.

Selective Permeabilization Reagents.

In various embodiments selectivity/specificity of the assays described herein is provided (at least in part) by the use of a selective permeabilization reagent that selectively permeabilizes or lyses the target microorganism.

Various permeabilization reagents can be used to selectively permeabilize or lyse the target microorganism. In certain embodiments the permeabilzation reagent comprises a reagent that is intrinsically selective for a particular (e.g., genus, species, strain, etc.) target microorganism. Such reagents include, for example, certain antimicrobial peptides (AMPs).

In certain embodiments, however, selectivity can be conferred by providing a targeting moiety (e.g., a target specific peptide, a target specific antibody, a target specific receptor ligand, etc.) attached to a moiety that permeabilizes or lyses a microorganism. The targeting moiety is selected to specifically or preferentially bind to the target microorganism thereby selectively delivering the permeabilizing moiety to the target microorganism. Suitable targeting moieties are described below.

Targeting Moieties.

In certain embodiments, the targeting moiety comprises one or more targeting peptides that bind particular bacteria, fungi, and/or yeasts, and/or algae, and/or viruses, and/or cells, and/or that bind particular groups of bacteria, and/or groups of fungi, and/or groups of yeasts, and/or groups of algae.

In certain embodiments the targeting peptides include peptides comprising or consisting of one or more of the amino acid sequences shown in Table 2 (SEQ ID NOs:1-1030). In various embodiments the peptides include peptides comprising or consisting of the retro, inverso, retro-inverso, and/or beta form of one or more of the amino acid sequences shown in Table 2. Also contemplated are circular permutations of these sequences as well as peptides comprising or consisting of the retro, inverso, retro-inverso, and/or beta form of such circular permutations. Also contemplated are targeting peptides comprising one, two, three four, or five conservative substitutions of these amino acid sequences.

TABLE 2

Illustrative list of novel targeting peptides.

SEQ ID

ID
Target(s)
Targeting Peptide Sequence
NO

1T-3

S. mutans, S. gordonii
VLGIAGGLDAYGELVGGN
1

1T-4

S. mutans, S. gordonii, S. sanguinis,
LDAYGELVGGN
2

S. oralis, V. atypica,

L. casei

1T-6

S. mutans

KFINGVLSQFVLERK
3

1T-7

M. xanthus

SQRIIEPVKSPQPYPGFSVS
4

1T-8

M. xanthus

FSVAACGEQRAVTFVLLIE
5

DLI

1T-9

M. xanthus

WAWAESPRCVSTRSNIHA
6

LAFRVEVAALT

1T-10

M. xanthus

SPAGLPGDGDEA
7

1T-11

S. mutans, S. epidermidis, P. aeruginosa
RISE
8

1T-12

C. xerosis, C. striatum, S. epidermidis,
FGNIFKGLKDVIETIVKWT
9

S. mutans

AAK

1T-13

S. aureus, S. epidermidis, P. aeruginosa
FRSPCINNNSLQPPGVYPAR
10

1T-14

S. mutans, S. aureus, S. epidermidis,
ALAGLAGLISGK
11

C. xerosis

1T-15

S. mutans

DVILRVEAQ
12

1T-16

P. aeruginosa

IDMR
13

1T-17

S. mutans

NNAIVYIS
14

1T-18

S. aureus, S. epidermidis, C. striatum,
YSKTLHFAD
15

P. aeruginosa

1T-19

S. aureus, S. epidermidis, P. aeruginosa
PGAFRNPQMPRG
16

1T-20

S. mutans, P. aeruginosa
PALVDLSNKEAVWAVLD
17

DHS

1T-21

S. mutans, P. aeruginosa
YVEEAVRAALKKEARIST
18

EDTPVNLPSFDC

1T-22

S. epidermidis, P. aeruginosa
VPLDDGTRRPEVARNRDK
19

DRED

1T-23

S. mutans, P. aeruginosa
PALVDLSNKEAVWAVLD
20

DHS

1T-24

P. aeruginosa

EEAEEKLAEVSQAVKRLVR
21

1T-25

S. aureus, S. epidermidis, C. xerosis,
VGLDVSVLVLFFGLQLLS
22

C. striatum, P. aeruginosa
VLLGAMIR

1T-26

S. mutans, S. aureus, S. epidermidis,
LTILPTTFFAIIVPILAVAFI
23

C. jeikeium, C. xerosis,
AYSGFKIKGIVEHKDQW

C. striatum, P. aeruginosa

1T-27

S. mutans, S. aureus, S. epidermidis,
ALFVSLEQFLVVVAKSVF
24

C. jeikeium, C. xerosis,
ALCHSGTLS

C. striatum, P. aeruginosa

1T-28

P. aeruginosa

VSRDEAMEFIDREWTTLQ
25

PAGKSHA

1T-29

S. mutans, S. aureus, S. epidermidis,
GSVIKKRRKRMSKKKHRK
26

C. jeikeium, C. xerosis,
MLRRTRVQRRKLGK

C. striatum, P. aeruginosa

1T-30

S. aureus, S. epidermidis, C. xerosis,
GKAKPYQVRQVLRAVDK
27

C. striatum, P. aeruginosa
LETRRKKGGR

1T-31

S. mutans, P. aeruginosa
NATGTDIGEVTLTLGRFS
28

1T-32

S. mutans

VSFLAGWLCLGLAAWRL
29

GNA

1T-33

S. aureus, S. epidermidis, C. jeikeium,
VRTLTILVIFIFNYLKSISY
30

C. xerosis, C. striatum,
KLKQPFENNLAQSMISI

P. aeruginosa

1T-34

S. aureus, S. epidermidis, C. jeikeium,
AFWLNILLTLLGYIPGIVH
31

C. xerosis, C. striatum,
AVYIIAKR

P. aeruginosa

1T-35

P. aeruginosa

EICLTLVFPIRGSYSEAAKF
32

PVPIHIVEDGTVELPK

1T-36

S. aureus, S. epidermidis, C. jeikeium,
VYRHLRFIDGKLVEIRLERK
33

C. xerosis, C. striatum,

P. aeruginosa

1T-37

S. mutans, S. aereus, S. epidermidis,
YIVGALVILAVAGLIYSML
34

C. jeikeium, C. xerosis,
RKA

C. striatum, P. aeruginosa

1T-38

S. mutans, S. aereus, S. epidermidis,
VMFVLTRGRSPRPMIPAY
35

C. jeikeium, C. xerosis,

C. striatum, P. aeruginosa

1T-39

S. mutans, P. aeruginosa
FGFCVWMYQLLAGPPGPPA
36

1T-40

S. mutans, P. aeruginosa
QRVSLWSEVEHEFR
37

1T-41

S. mutans, S. aureus, S. epidermidis,
KRGSKIVIAIAVVLIVLAG
38

C. jeikeium, C. striatum,
VWVW

P. aeruginosa

1T-42

S. aureus, S. epidermidis, C. xerosis,
TVLDWLSLALATGLFVYL
39

C. striatum, P. aeruginosa
LVALLRADRA

1T-43

C. jeikium, P. aeruginosa
DRCLSVLSWSPPKVSPLI
40

1T-44

S. mutans, S. aureus, S. epidermidis,
DPALADFAAGMRAQVRT
41

C. jeikeium, C. striatum,

P. aeruginosa

1T-45

S. aureus, S. epidermidis, C. striatum,
WTKPSFTDLRLGFEVTLY
42

P. aeruginosa

FANR

1T-46

S. aureus, S. epidermidis, C. jeikeium,
FSFKQRVMFRKEVERLR
43

C. xerosis, C. striatum,

P. aeruginosa

1T-47

S. mutans, S. epidermidis, P. aeruginosa
VIKISVPGQVQMLIP
44

1T-48

S. aureus, S. epidermidis, C. jeikeium,
KLQVHHGRATHTLLLQPP
45

C. xerosis, C. striatum,
LCAPGTIR

P. aeruginosa

1T-49

S. aureus, S. epidermidis, C. jeikeium,
SLVRIHDQQPWVTRGAFI
46

P. aeruginosa

DAARTCS

1T-50

P. aeruginosa

HSDEPIPNILFKSDSVH
47

1T-51

S. aureus, P. aeruginosa
GKPKRMPAEFIDGYGQAL
48

LAGA

1T-52

S. aureus, C. xerosis, P. aeruginosa
DEYPAKLPLSDKGATEPR
49

RH

1T-53

P. aeruginosa

SDILAEMFEKGELQTLVK
50

DAAAKANA

1T-54

S. epidermidis, C. xerosis, C. striatum,
RWVSCNPSWRIQ
51

P. aeruginosa

1T-55

C. xerosis, P. aeruginosa
NHKTLKEWKAKWGPEAV
52

ESWATLLG

1T-56

C. xerosis, P. aeruginosa
LALIGAGIWMIRKG
53

1T-57

P. aeruginosa

RLEYRRLETQVEENPESG
54

RRPMRG

1T-58

P. aeruginosa

CDDLHALERAGKLDALLSA
55

1T-59

S. aureus, S. epidermidis, P. aeruginosa
AVGNNLGKDNDSGHRGK
56

KHRKHKHR

1T-60

S. aureus, S. epidermidis, C. jeikeium,
YLTSLGLDAAEQAQGLLT
57

C. striatum, P. aeruginosa
ILKG

1T-61

P. aeruginosa

HATLLPAVREAISRQLLPA
58

LVPRG

1T-62

S. epidermidis, P. aeruginosa
GCKGCAQRDPCAEPEPYF
59

RLR

1T-63

S. aureus, S. epidermidis, C. jeikeium,
EPLILKELVRNLFLFCYAR
60

C. xerosis, C. striatum,
ALR

P. aeruginosa

1T-64

S. aureus, S. epidermidis, C. jeikeium,
QTVHHIHMHVLGQRQMH
61

C. xerosis, C. striatum,
WPPG

P. aeruginosa

1T-65

S. mutans, S. aureus, S. epidermidis,
HARAAVGVAELPRGAAV
62

C. jeikeium, C. xerosis,
EVELIAAVRP

C. striatum, P. aeruginosa

1T-66

S. mutans, S. aureus, S. epidermidis,
DTDCLSRAYAQRIDELDK
63

C. jeikeium, C. xerosis,
QYAGIDKPL

C. striatum, P. aeruginosa

1T-67

S. aureus, S. epidermidis, C. jeikeium,
GQRQRLTCGRVSGCSEGP
64

C. xerosis, C. striatum,
SREAAR

P. aeruginosa

1T-68

S. mutans, S. aureus, C. jeikeium,
GGTKEIVYQRG
65

C. xerosis, C. striatum,

P. aeruginosa

1T-69

S. mutans, P. aeruginosa
ILSQEADRKKLF
66

1T-70

S. aureus, C. jeikeium, P. aeruginosa
NRQAQGERAHGEQQG
67

1T-71

P. aeruginosa

KIDTNQWPPNKEG
68

1T-72

P. aeruginosa

EPTDGVACKER
69

1T-73

S. pneumoniae

GWWEELLHETILSKFKITK
70

ALELPIQL

1T-74

S. pneumoniae

DIDWGRKISCAAGVAYGA
71

IDGCATTV

1T-75

S. pneumoniae

GVARGLQLGIKTRTQWG
72

AATGAA

1T-76

S. pneumoniae

EMRLSKFFRDFILWRKK
73

1T-77

S. pneumoniae

EMRISRIILDFLFLRKK
74

1T-78

S. pneumoniae

FFKTIFVLILGALGVAAGL
75

YIEKNYIDK

1T-79

S. pneumoniae

FGTPWSITNFWKKNFNDR
76

PDFDSDRRRY

1T-80

S. pneumoniae

GGNLGPGFGVIIP
77

1T-81

S. pneumoniae

AIATGLDIVDGKFDGYLWA
78

1T-82

S. pneumoniae

FGVGVGIALFMAGYAIGK
79

DLRKKFGKSC

1T-83

S. pneumoniae

QKPRKNETFIGYIQRYDID
80

GNGYQSLPCPQN

1T-84

S. pneumoniae

FRKKRYGLSILLWLNAFT
81

NLVNSIHAFYMTLF

1T-85

A. naeslundii, F. nucleatum, P. gingivalis,
VMASLTWRMRAASASLP
82

S. epidermidis, S. gordonii,
THSRTDA

S. mitis, S. mutans, S. oralis,

S. salivarious, S. sanguinis

1T-86

S. mitis, S. oralis, S. salivarious
HRKNPVLGVGRRHRAHN
83

VA

1T-87

S. mitis, S. mutans, S. oralis
EAVGQDLVDAHHP
84

1T-89

S. mitis, S. mutans
HEDDKRRGMSVEVLGFE
85

VVQHEE

1T-90

S. gordonii, S. mitis, S. mutans,
RNVIGQVL
86

S. oralis, S. sanguinis

1T-91

S. mitis, S. mutans, S. oralis, S. sanguinis
TSVRPGAAGAAVPAGAA
87

GAAGAGWRWP

1T-92

S. mitis, S. mutans
GQDEGQRRAGVGEGQGV
88

DG

1T-93

S. epidermidis, S. gordonii, S. mitis,
AMRSVNQA
89

S. mutans, S. oralis, S. sanguinis

1T-94

S. mitis, S. mutans, S. oralis
DQVAHSGDMLVQARRRDS
90

1T-95

S. gordonii, S. mitis, S. mutans,
GHLLRVGGRVGGVGGVA
91

S. oralis, S. sanguinis
GACAQPFGGQ

1T-96

S. gordonii, S. mitis, S. mutans,
VAGACAQPFGGQ
92

S. oralis, S. sanguinis

1T-97

A. naeslundii, F. nucleatum, P. gingivalis,
GVAERNLDRITVAVAIIWT
93

S. epidermidis, S. gordonii,
ITIVGLGLVAKLG

S. mitis, S. mutans, S. oralis,

S. salivarious, S. sanguinis

1T-98

A. naeslundii, F. nucleatum, P. gingivalis,
VRSAKAVKALTAAGYTG
94

S. epidermidis, S. gordonii,
ELVNVSGGMKAWLGQ

S. mitis, S. mutans, S. oralis,

S. salivarious, S. sanguinis

1T-99

S. gordonii, S. mitis, S. mutans,
MKAWLGQ
95

S. oralis, S. sanguinis

1T-100

S. gordonii, S. mitis, S. mutans
LDPLEPRIAPPGDRSHQGA
96

PACHRDPLRGRSARDAER

1T-101

A. naeslundii, P. gingivalis, S. epidermidis,
RLRVGRATDLPLTSFAVG
97

S. gordonii, S. mitis,
VVRNLPDAPAH

S. mutans, S. oralis, S. sanguinis

1T-102

A. naeslundii, F. nucleatum, P. gingivalis,
WKRLWPARILAGHSRRR
98

S. epidermidis, S. gordonii,
MRWMVVWRYFAAT

S. mitis, S. mutans, S. oralis,

S. salivarious, S. sanguinis

1T-103

A. naeslundii, F. nucleatum, P. gingivalis,
AQFYEAIITGYALGAGQRI
99

S. epidermidis, S. gordonii,
GQL

S. mitis, S. mutans, S. oralis,

S. sanguinis

1T-104

S. mitis

RAVAAHLQGRHHGHQVR
100

RQRHGQR

1T-105

S. epidermidis, S. gordonii, S. mitis,
GEGLPPPVLHLPPPRMSGR
101

S. mutans, S. oralis

1T-106

S. gordonii, S. mitis, S. mutans,
DALRRSRSQGRRHR
102

S. oralis, S. salivarious

1T-107

A. naeslundii, S. epidermidis, S. gordonii,
SPVPRFTAVGGVSRGSP
103

S. mitis, S. mutans, S. oralis,

S. salivarious, S. sanguinis

1T-108

S. gordonii, S. mitis, S. mutans,
WGPLGPERPLW
104

S. oralis, S. salivarious, S. sanguinis

1T-109

A. naeslundii, S. epidermidis, S. gordonii,
VTTNVRQGAGS
105

S. mitis, S. mutans, S. oralis,

S. salivarious, S. sanguinis

1T-110

A. naeslundii, P. gingivalis, S. epidermidis,
LAAKTAVCVGRAFM
106

S. gordonii, S. mitis,

S. mutans, S. oralis, S. sanguinis

1T-111

A. naeslundii, F. nucleatum, P. gingivalis,
GRLSRREEDPATSIILLRG
107

S. epidermidis, S. gordonii,
AYRMAVF

S. mitis, S. mutans, S. oralis,

S. salivarious, S. sanguinis

1T-112

S. gordonii

SDNDGKLILGTSQ
108

1T-113

S. mitis

HGAHQRTGQRLHHHRGR
109

TVSGCRQNPVAGVDPDEHR

1T-114

A. naeslundii, P. gingivalis, S. epidermidis,
RQAPGPGLVTITAACSAPG
110

S. gordonii, S. mitis,
SRSR

S. mutans, S. oralis, S. sanguinis

1T-115

A. naeslundii, F. nucleatum, P. gingivalis,
LLIERFSNHH
111

S. epidermidis, S. gordonii,

S. mitis, S. mutans, S. oralis,

S. salivarious, S. sanguinis

1T-116

A. naeslundii, P. gingivalis, S. epidermidis,
MILHRRRDR
112

S. gordonii, S. mitis,

S. mutans, S. oralis, S. salivarious,

S. sanguinis

1T-117

S. mutans

GPGVVGPAPFSRLPAHAL
113

NL

1T-118

A. naeslundii, F. nucleatum, P. gingivalis,
TASPPAPSDQGLRTAFPAT
114

S. epidermidis, S. gordonii,
LLIALAALARISR

S. mitis, S. mutans, S. oralis,

S. salivarious, S. sanguinis

1T-119

S. gordonii, S. mitis, S. mutans,
SPATQKAPTRAQPSRAPV
115

S. oralis

QDCGDGRPTAAPDDVERL

SPR

1T-120

A. naeslundii, F. nucleatum, P. gingivalis,
DVRDRVDLAGADLCAAH
116

S. epidermidis, S. gordonii,
ATR

S. mitis, S. mutans, S. oralis,

S. salivarious, S. sanguinis

1T-121

S. gordonii, S. mitis, S. mutans,
FAKETGFGIGGAQEGWWI
117

S. oralis, S. salivarious, S. sanguinis
IADIYGPNPF

1T-122

S. mitis

GAIPDPVTHRVDWEEDHQ
118

TRPSR

1T-123

S. gordonii

LVRRNAVAGRSDGLAGA
119

EQLDLVRLQGVL

1T-124

S. mitis, S. mutans, S. oralis
LFDERNKIA
120

1T-125

S. epidermidis, S. gordonii, S. mutans,
DAITGGNPPLSDTDGLRP
121

S. oralis

1T-126

S. gordonii, S. mitis, S. mutans
QGLARPVLRRIPL
122

1T-127

A. naeslundii, F. nucleatum, P. gingivalis,
YDPVPKRKNKNSEGKREE
123

T. denticola, S. gordonii,

S. mitis, S. mutans, S. oralis,

S. salivarious, S. sanguinis

1T-128

A. naeslundii, P. gingivalis, S. epidermidis,
SGSAIRMLEIATKMLKR
124

S. gordonii, S. mitis,

S. mutans, S. oralis, S. salivarious,

S. sanguinis

1T-129

A. naeslundii, P. gingivalis, S. epidermidis,
YDKYIKYLSIQPPFIVYFI
125

S. gordonii, S. mitis,

S. mutans, S. oralis, S. salivarious,

S. sanguinis

1T-130

A. naeslundii, F. nucleatum, P. gingivalis,
QKIIDMSKFLFSLILFIMIV
126

S. epidermidis, S. gordonii,
VIYIGKSIGGYSAIVSSIML

S. mitis, S. mutans, S. oralis,
ELDTVLYNKKIFFIYK

S. salivarious, S. sanguinis

1T-131

A. naeslundii, F. nucleatum, P. gingivalis,
DEVWKMLGI
127

T. denticola, S. gordonii,

S. mitis, S. mutans, S. oralis,

S. salivarious, S. sanguinis

1T-132

A. naeslundii, F. nucleatum, P. gingivalis,
YSKKLFEYFYFIIFILIRYLI
128

S. epidermidis, S. gordonii,
FYKIIQNKNYYINNIAYN

S. mitis, S. mutans, S. oralis,

S. salivarious, S. sanguinis

1T-133

A. naeslundii, P. gingivalis, S. epidermidis,
YFIKDDNEALSKDWEVIG
129

S. gordonii, S. mitis,
NDLKGTIDKYGKEFKVR

S. mutans, S. oralis, S. salivarious,

S. sanguinis

1T-134

A. naeslundii, F. nucleatum, P. gingivalis,
SRLVREIKKKCRKS
130

S. epidermidis, S. gordonii,

S. mitis, S. mutans, S. oralis,

S. salivarious, S. sanguinis

1T-135

A. naeslundii, P. gingivalis, S. epidermidis,
FESLLPQATKKIVNNKGSK
131

S. gordonii, S. mitis,
INKIF

S. mutans, S. oralis, S. salivarious,

S. sanguinis

1T-136

A. naeslundii, F. nucleatum, P. gingivalis,
ELLTQIRLALLYSVNEW
132

S. epidermidis, S. gordonii,

S. mitis, S. mutans, S. oralis,

S. salivarious, S. sanguinis

1T-137

A. naeslundii, F. nucleatum, P. gingivalis,
PLNFYRAVKENRLPLSEK
133

S. epidermidis, S. gordonii,
NINDFTNIKLKVSPKLINLL

S. mitis, S. mutans, S. oralis,
QESSIFYNFSPKKRNTN

S. salivarious, S. sanguinis

1T-138

A. naeslundii, F. nucleatum, P. gingivalis,
YPNEYCIFLENLSLEELKEI
134

S. epidermidis, S. gordonii,
KAINGETLNLEEIINERKN

S. mitis, S. mutans, S. oralis,
LKD

S. salivarious, S. sanguinis

1T-139

A. naeslundii, S. gordonii, S. mitis,
AVAGAAVGALLGNDARS
135

S. mutans, S. oralis
TAVGAAIGGALGAGAGEL

TKNK

1T-140

A. naeslundii, F. nucleatum, P. gingivalis,
IKGTIAFVGEDYVEIRVDK
136

S. epidermidis, S. gordonii,
GVKLTFRKSAIANVINNN

S. mitis, S. mutans, S. oralis,
QQ

S. salivarious, S. sanguinis

1T-141

F. nucleatum, P. gingivalis, S. epidermidis,
KKFIILLFILVQGLIFSATK
137

S. gordonii, S. mitis,
TLSDIIAL

S. mutans, S. oralis, S. sanguinis

1T-142

A. naeslundii, F. nucleatum, P. gingivalis,
FTQGIKRIVLKRLKED
138

T. denticola, S. gordonii,

S. mitis, S. mutans, S. oralis,

S. salivarious, S. sanguinis

1T-143

A. naeslundii, F. nucleatum, P. gingivalis,
MPKRHYYKLEAKALQFG
139

S. epidermidis, S. gordonii,
LPFAYSPIQLLK

S. mitis, S. mutans, S. oralis,

S. salivarious, S. sanguinis

1T-144

A. naeslundii, F. nucleatum, P. gingivalis,
IIELHPKSWTQDWRCSFL
140

T. denticola, S. gordonii,

S. mitis, S. mutans, S. oralis,

S. salivarious, S. sanguinis

1T-145

S. mitis, S. mutans, S. oralis
VEAGKRNISLENIEKISKG
141

LGISISELFKYIEEGEDKIG

1T-146

A. naeslundii, F. nucleatum, P. gingivalis,
RNSADNQTKIDKIRIDISL
142

T. denticola, S. gordonii,
WDEHLNIVKQGK

S. mitis, S. mutans, S. oralis,

S. salivarious, S. sanguinis

1T-147

A. naeslundii, F. nucleatum, P. gingivalis,
GVENRRFYERDVSKVSM
143

T. denticola, S. gordonii,
MTSEAVAPRGGSK

S. mitis, S. mutans, S. oralis,

S. salivarious, S. sanguinis

1T-148

A. naeslundii, F. nucleatum, P. gingivalis,
IVELDDTTILERALSMLGE
144

T. denticola, S. gordonii,
ANA

S. mitis, S. mutans, S. oralis,

S. salivarious, S. sanguinis

1T-149

A. naeslundii, F. nucleatum, P. gingivalis,
SVRAVKPIDETVARHFPG
145

T. denticola, S. gordonii,
DFIVN

S. mitis, S. mutans, S. oralis,

S. salivarious, S. sanguinis

1T-150

A. naeslundii, F. nucleatum, P. gingivalis,
YINRRLKKAFSDADIKEAP
146

T. denticola, S. gordonii,
AEFYEELRRVQYV

S. mitis, S. mutans, S. oralis,

S. salivarious, S. sanguinis

1T-151

A. naeslundii, F. nucleatum, P. gingivalis,
SVRAVKPIDEIVAWHFPG
147

T. denticola, S. gordonii,
DFIVN

S. mitis, S. mutans, S. oralis,

S. salivarious, S. sanguinis

1T-152

A. naeslundii, F. nucleatum, P. gingivalis,
YVSADESAYNHIVTDDIPL
148

S. epidermidis, S. gordonii,
ADRRIEAVQQ

S. mitis, S. mutans, S. oralis,

S. salivarious, S. sanguinis

1T-153

A. naeslundii, F. nucleatum, P. gingivalis,
YIACPGYFY
149

S. epidermidis, S. gordonii,

S. mitis, S. mutans, S. oralis,

S. salivarious, S. sanguinis

1T-154

P. gingivalis

YFSFLEIVGMARR
150

1T-155

A. naeslundii, F. nucleatum, P. gingivalis,
LKLAFGVYPFQAMSQSDT
151

S. epidermidis, S. gordonii,
AVSERNVLWR

S. mitis, S. mutans, S. oralis,

S. salivarious, S. sanguinis

1T-156

A. naeslundii, F. nucleatum, P. gingivalis,
GRFQISIRGEEKSKVKVQG
152

T. denticola, S. gordonii,
KGTFTDRNTT

S. mitis, S. mutans, S. oralis,

S. salivarious, S. sanguinis

1T-157

A. naeslundii, F. nucleatum, P. gingivalis,
RRFRKTTENREKSKNKKA
153

T. denticola, S. gordonii,
VLGLSTTSTASY

S. mitis, S. mutans, S. oralis,

S. salivarious, S. sanguinis

1T-158

A. naeslundii, F. nucleatum, P. gingivalis,
WENKPSPLGSIKKLQGLV
154

S. epidermidis, S. gordonii,
YRLIGYRHFWV

S. mitis, S. mutans, S. oralis,

S. salivarious, S. sanguinis

1T-159

P. gingivalis

IFSLHHFALICSEMGTFAV
155

SKRAKYKWEVL

1T-160

A. naeslundii, F. nucleatum, P. gingivalis,
AQYKYINKLLN
156

T. denticola, S. gordonii,

S. mitis, S. mutans, S. oralis,

S. salivarious, S. sanguinis

1T-161

A. naeslundii, F. nucleatum, P. gingivalis,
NKVLQVEVMWDGSVVGR
157

S. epidermidis, S. gordonii,
PAGVISIKSSKKG

S. mitis, S. mutans, S. oralis,

S. salivarious, S. sanguinis

1T-162

A. naeslundii, F. nucleatum, P. gingivalis,
QKAKEESDRKAAVSYNGF
158

T. denticola, S. gordonii,
HRVNVVSIPK

S. mitis, S. mutans, S. oralis,

S. salivarious, S. sanguinis

1T-163

A. naeslundii, F. nucleatum, P. gingivalis,
MENILIYIPMVLSPFGSGIL
159

S. epidermidis, S. gordonii,
LFLGKDRRYML

S. mitis, S. mutans, S. oralis,

S. salivarious, S. sanguinis

1T-164

A. naeslundii, F. nucleatum, P. gingivalis,
KKSHSQGKRKLKDLNSAY
160

S. epidermidis, S. gordonii,
KIDNQLHYALR

S. mitis, S. mutans, S. oralis,

S. salivarious, S. sanguinis

1T-165

A. naeslundii, F. nucleatum, P. gingivalis,
CYDSFDFSIFVTFANRMKL
161

S. epidermidis, S. gordonii,
SVGS

S. mitis, S. mutans, S. oralis,

S. salivarious, S. sanguinis

1T-166

A. naeslundii, F. nucleatum, P. gingivalis,
AQSAGQIKRKSKVRIHV
162

S. epidermidis, S. gordonii,

S. mitis, S. mutans, S. oralis,

S. salivarious, S. sanguinis

1T-167

A. naeslundii, F. nucleatum, P. gingivalis,
SRMSEHSPAGLVFEVGPM
163

S. epidermidis, S. gordonii,
DKGSFIILDSYHPTVKK

S. mitis, S. mutans, S. oralis,

S. salivarious, S. sanguinis

1T-168

A. naeslundii, F. nucleatum, P. gingivalis,
ELHRIMSTEKIGAVTKMN
164

S. epidermidis, S. gordonii,
FDTAPIMSILPIDIYPKEVGI

S. mitis, S. mutans, S. oralis,
GS

S. salivarious, S. sanguinis

1T-169

A. naeslundii, F. nucleatum, P. gingivalis,
FARVRRLHQNRILTQPLTN
165

S. epidermidis, S. gordonii,
LKYCLRQPIYSD

S. mitis, S. mutans, S. oralis,

S. salivarious, S. sanguinis

1T-170

P. gingivalis

AYGKVFSMDIMLSENDKL
166

IVLRISHHSAWH

1T-171

A. naeslundii, F. nucleatum, P. gingivalis,
SVRAVKPIDKTVARHFPG
167

S. epidermidis, S. gordonii,
DFIVN

S. mitis, S. mutans, S. oralis,

S. salivarious, S. sanguinis

1T-172

A. naeslundii, F. nucleatum, P. gingivalis,
FEGLKNLLGDDII
168

S. epidermidis, S. gordonii,

S. mitis, S. mutans, S. oralis,

S. salivarious, S. sanguinis

1T-173

A. naeslundii, F. nucleatum, P. gingivalis,
LFRKEDQEHVLL
169

S. gordonii, S. mitis,

S. mutans, S. oralis, S. salivarious,

S. sanguinis

1T-174

A. naeslundii, F. nucleatum, P. gingivalis,
SGGSDTDGSSSGEPGSHSG
170

T. denticola, S. gordonii,
DL

S. mitis, S. mutans, S. oralis,

S. salivarious, S. sanguinis

1T-175

A. naeslundii, F. nucleatum, P. gingivalis,
GEPGSHSGDL
171

S. epidermidis, S. gordonii,

S. mitis, S. mutans, S. oralis,

S. salivarious, S. sanguinis

1T-176

A. naeslundii, P. gingivalis, S. epidermidis,
PVGDIMSGFLRGANQPRF
172

S. gordonii, S. mitis,
LLDHISFGS

S. mutans, S. oralis, S. salivarious,

S. sanguinis

1T-177

P. gingivalis, S. gordonii, S. mitis,
GTNVPTQILGYSREERFDY
173

S. mutans, S. oralis, S. salivarious,
EPAPEQR

S. sanguinis

1T-178

A. naeslundii, F. nucleatum, P. gingivalis,
LLASHPERLSLGVFFVYRV
174

S. epidermidis, S. gordonii,
LHLLLENT

S. mitis, S. mutans, S. oralis,

S. salivarious, S. sanguinis

1T-179

A. naeslundii, F. nucleatum, P. gingivalis,
TCYPLIQRKTDRAYEA
175

T. denticola, S. gordonii,

S. mitis, S. mutans, S. oralis,

S. salivarious, S. sanguinis

1T-180

A. naeslundii, F. nucleatum, P. gingivalis,
VVFGGGDRLV
176

T. denticola, S. gordonii,

S. mitis, S. mutans, S. oralis,

S. salivarious, S. sanguinis

1T-181

A. naeslundii, F. nucleatum, P. gingivalis,
YGKESDP
177

T. denticola, S. gordonii,

S. mitis, S. mutans, S. oralis,

S. salivarious, S. sanguinis

1T-182

A. naeslundii, F. nucleatum, P. gingivalis,
LTASICRQWNDNSTPYQR
178

T. denticola, S. gordonii,

S. mitis, S. mutans, S. oralis,

S. salivarious, S. sanguinis

1T-183

A. naeslundii, F. nucleatum, P. gingivalis,
PLRSFVAEKAEHAFRVVRI
179

S. epidermidis, S. gordonii,
ADFDFGHS

S. mitis, S. mutans, S. oralis,

S. salivarious, S. sanguinis

1T-184

A. naeslundii, F. nucleatum, P. gingivalis,
ALLVLNLLLMQFFFGKNM
180

T. denticola, S. gordonii,

S. mitis, S. mutans, S. oralis,

S. salivarious, S. sanguinis

1T-185

A. naeslundii, F. nucleatum, P. gingivalis,
HYHFLLEFGFHKGDYLE
181

T. denticola, S. gordonii,

S. mitis, S. mutans, S. oralis,

S. salivarious, S. sanguinis

1T-188

S. sanguinis

HRKDVYKK
182

1T-190

A. naeslundii, F. nucleatum, P. gingivalis,
IQIIVNAFVEKDKTGAVIE
183

S. epidermidis, S. gordonii,
VLYASNNHEKVKAKYEE

S. mitis, S. mutans, S. oralis,
LVAIS

S. salivarious, S. sanguinis

1T-192

S. sanguinis

ILVLLALQVELDSKFQY
184

1T-193

S. sanguinis

LMIFDKHANLKYKYGNRS
185

FGVEAIM

1T-195

S. mutans

AASGFTYCASNGVWHPY
186

1T-196

F. nucleatum, S. sanguinis
KPEKEKLDTNTLMKVVN
187

KALSLFDRLLIKFGA

1T-197

A. naeslundii, F. nucleatum, P. gingivalis,
TEILNFLITVCADRENWKI
188

S. epidermidis, S. gordonii,
KHGLSDSVLLIFFARFTGA

S. mitis, S. mutans, S. oralis,
EYW

S. salivarious, S. sanguinis

1T-198

P. gingivalis, S. epidermidis, S. gordonii,
MPVSKKRYMLSSAYATA
189

S. mitis, S. mutans, S. oralis,
LGICYGQVATDEKESEITA

S. sanguinis

IPDLLDYLSVEEYLL

1T-199

S. sanguinis

RAGRIKKLSQKEAEPFEN
190

1T-200

A. naeslundii, F. nucleatum, S. epidermidis,
MRFKRFDRDYALSGDNV
191

S. gordonii, S. mitis,
FEVLTASCDVIERNLSYRE

S. mutans, S. oralis, S. salivarious,
MCGLMQ

S. sanguinis

1T-201

S. sanguinis

KRKHENVIVAEEMRVIKN
192

1T-202

A. naeslundii, F. nucleatum, P. gingivalis,
LCRLEKLCKQFLRQDKVV
193

S. epidermidis, S. gordonii,
TYYLMLPYKRAIEAFYQE

S. mitis, S. mutans, S. oralis,
LKERS

S. salivarious, S. sanguinis

1T-203

A. naeslundii, F. nucleatum, P. gingivalis,
YPFCLATVDHLPEGLSVT
194

S. epidermidis, S. gordonii,
DYERVQRLVSQFLLNKEER

S. mitis, S. mutans, S. oralis,

S. salivarious, S. sanguinis

1T-207

F. nucleatum, S. sanguinis
SPLEKYGTGSMTALTFLL
195

GCCLLVLSKKSR

1T-208
Unanalyzed
KRKRWAILTLFLAGLGAV
196

GIVLATF

1T-215

S. sanguinis

VCFKDISVFLSPFRGQEVL
197

FCGKAKHSLIYVIGT

1T-216

S. sanguinis

FFLNVIAIRIPHF
198

1T-217

F. nucleatum, S. sanguinis
MLSNVLSRSVVSPNVDIP
199

NSMVILSPLLISISNYH

1T-218

F. nucleatum, S. sanguinis
KLIFAALGLVFLLIGLRDS
200

RSK

1T-219

S. sanguinis

RNINVSATFITEKSLV
201

1T-221

A. naeslundii, F. nucleatum, P. gingivalis,
DIGRIIGKKGRTITAIRSIV
202

S. epidermidis, S. gordonii,
YSVPTQGKKVRLVIDEK

S. mitis, S. mutans, S. oralis,

S. salivarious, S. sanguinis

1T-222

F. nucleatum, S. sanguinis
RIEASLISAIMFSMFNAIVK
203

FLQK

1T-223

A. naeslundii, F. nucleatum, P. gingivalis,
NQKMEINSMTSEKEKMLA
204

S. epidermidis, S. gordonii,
GHFHNEANFAVIFKYSLF

S. mitis, S. mutans, S. oralis,
YNFF

S. salivarious, S. sanguinis

1T-225

A. naeslundii, F. nucleatum, P. gingivalis,
RRSLGNSASFAEWIEYIRY
205

S. epidermidis, S. gordonii,
LHYIIRVQFIHFFSKNKKI

S. mitis, S. mutans, S. oralis,

S. salivarious, S. sanguinis

1T-226

A. naeslundii, F. nucleatum, S. epidermidis,
KLQEKQIDRNFERVSGYS
206

S. gordonii, S. mitis,
TYRAVQAAKAKEKGFISL

S. mutans, S. oralis, S. salivarious,
EN

S. sanguinis

1T-228

A. naeslundii, F. nucleatum, P. gingivalis,
IFKLFEEHLLYLLDAFYYS
207

S. epidermidis, S. gordonii,
KIFRRLKQGLYRRKEQPY

S. mitis, S. mutans, S. oralis,
TQDLFRM

S. salivarious, S. sanguinis

1T-230

A. naeslundii, F. nucleatum, P. gingivalis,
EFLEKFKVLKQPRKANNIS
208

S. epidermidis, S. gordonii,
KNRVAMIFLTIHKSRGFLS

S. mitis, S. mutans, S. oralis,
SPY

S. salivarious, S. sanguinis

1T-233

A. naeslundii, P. gingivalis, S. epidermidis,
TDQELEHLIVTELESKRLD
209

S. gordonii, S. mitis,
FTYSKDITEFFDEAFPEYD

S. mutans, S. oralis, S. salivarious,
QNY

S. sanguinis

1T-234

A. naeslundii, F. nucleatum, P. gingivalis,
DNFYLILKMEERGKSKKT
210

S. epidermidis, S. gordonii,
SQTRGFRAFFDIIRKKIKKE

S. mitis, S. mutans, S. oralis,
DGK

S. salivarious, S. sanguinis

1T-237

S. sanguinis

EDPVPNHFTLRRNKKEKP
211

SKS

1T-238

A. naeslundii, F. nucleatum, P. gingivalis,
IFNRRKFFQYFGLSKEAM
212

S. epidermidis, S. gordonii,
VEHIQPFILDIWQIHLF

S. mitis, S. mutans, S. oralis,

S. salivarious, S. sanguinis

1T-239

A. naeslundii, S. gordonii, S. mitis,
ADDLLNKRLTDLIMENAE
213

S. mutans, S. oralis, S. sanguinis
TVKTIDLDNSD

1T-240

A. naeslundii, F. nucleatum, P. gingivalis,
VILGNGISNIAQTLGQLPNI
214

S. epidermidis, S. gordonii,
AWVWIYMVLIAALLEESN

S. mitis, S. mutans, S. oralis,
VC

S. salivarious, S. sanguinis

1T-242

F. nucleatum, S. sanguinis
KQVQNTTLIICGTVLLGIL
215

FKSYLKSQKSV

1T-243

A. naeslundii, P. gingivalis, S. epidermidis,
SENIARFAAAFENEQVVS
216

S. gordonii, S. mitis,
YARWFRRSWRGSGSSSRF

S. mutans, S. oralis, S. salivarious,

S. sanguinis

1T-248

S. sanguinis

IGGALNSCG
217

1T-249

F. nucleatum, S. sanguinis
VFSVLKHTTWPTRKQSW
218

HDFISILEYSAFFALVIFIFD

KLLTLGLAELLKRF

1T-250

S. mitis, S. mutans, S. oralis
LVQGDTILIENHVGTPVKD
219

DGKDCLIIREADVLAVVND

1T-252

F. nucleatum, S. sanguinis
MKKNLKRFYALVLGFIIG
220

CLFVSILIFIGY

1T-253

A. naeslundii, F. nucleatum, P. gingivalis,
KTKESLTQQEKKFLKDYD
221

S. epidermidis, S. gordonii,
RKSLHHFRDILTYCFILDK

S. mitis, S. mutans, S. oralis,
LTNK

S. salivarious, S. sanguinis

1T-256

S. sanguinis

KGKSLMPLLKQINQWGKL
222

YL

1T-257

A. naeslundii, F. nucleatum, P. gingivalis,
IILAKAADLAEIERIISEDPF
223

S. epidermidis, S. gordonii,
KINEIANYDIIEFCPTKSSK

S. mitis, S. mutans, S. oralis,
AFEKVLK

S. salivarious, S. sanguinis

1T-258

A. naeslundii, F. nucleatum, P. gingivalis,
TINIDDKVLDYLKKINSKA
224

T. denticola, S. mitis,
ITIDLIGCAS

S. mutans, S. oralis

1T-259

F. nucleatum, P. gingivalis, T. denticola,
EKLKKILLKLAVCGKAWY
225

S. mitis, S. mutans, S. oralis,
TL

S. sanguinis

1T-260

A. naeslundii, P. gingivalis, S. epidermidis,
NILYFIHDENQWEPQKAEI
226

S. gordonii, S. mitis,
FRGSIKHCAWLSS

S. mutans, S. oralis, S. sanguinis

1T-261

F. nucleatum, S. mutans, S. oralis,
SFEKNKIENNLKIAQAYIYI
227

S. sanguinis

KPKPRICQA

1T-262

A. naeslundii, F. nucleatum, P. gingivalis,
LSLPLIVLTKSI
228

S. epidermidis, S. gordonii,

S. mitis, S. mutans, S. oralis,

S. salivarious, S. sanguinis

1T-263

A. naeslundii, F. nucleatum, P. gingivalis,
FIAVSFTGNPATFKLVIGC
229

S. epidermidis, S. gordonii,
KADN

S. mitis, S. oralis, S. salivarious,

S. sanguinis

1T-264

S. sanguinis

LEGKFYMAEDFDKTPECF
230

KDYV

1T-265

A. naeslundii, F. nucleatum, P. gingivalis,
GMFENLLMINFQIMNDLK
231

S. epidermidis, S. gordonii,
IEIVVKDRICAV

S. mitis, S. mutans, S. oralis,

S. salivarious, S. sanguinis

1T-266

S. sanguinis

RAGTWLVVDEIR
232

1T-267

A. naeslundii, F. nucleatum, P. gingivalis,
RIKEERKNRSYKFFIWRLF
233

T. denticola, S. mitis,
DEKTGFI

S. mutans, S. oralis, S. sanguinis

1T-268

F. nucleatum, S. mutans, S. oralis,
PITPKKEKCGLGTYAPKNP
234

S. sanguinis

VFSKSRV

1T-269

F. nucleatum, S. mutans, S. oralis,
PLYVAAVEKINTAKKH
235

S. sanguinis

1T-270

F. nucleatum, S. mutans, S. oralis,
VHEFDIQKILQNR
236

S. sanguinis

1T-271

A. naeslundii, F. nucleatum, P. gingivalis,
FLIQKFLLIKTFPPYRKKY
237

S. epidermidis, S. gordonii,
VVIVSQTGTA

S. mitis, S. mutans, S. oralis,

S. salivarious, S. sanguinis

1T-272

F. nucleatum, S. mutans, S. oralis,
QLAPIDKQLKAVKKIAFY
238

S. sanguinis

ESESTAAKAVTVA

1T-273

F. nucleatum, P. gingivalis, T. denticola,
YNEPNYKWLESYKIYKQR
239

S. mitis, S. mutans, S. oralis
CEDRTGMYYTEET

1T-274

F. nucleatum, S. mutans, S. oralis,
ETTTEINAIKLHRIKQRSPQ
240

S. sanguinis

GTRRVN

1T-275

A. naeslundii, F. nucleatum, P. gingivalis,
QVLKNFSISRRYKINNPFF
241

T. denticola, S. epidermidis,
KILLFIQLRTL

S. gordonii, S. mitis,

S. mutans, S. oralis, S. salivarious,

S. sanguinis

1T-276

A. naeslundii, F. nucleatum, P. gingivalis,
ILTLLILGSIGFFILKIKLKL
242

S. epidermidis, S. gordonii,
GRF

gordonii, S. mitis, S. mutans, S. oralis,

S. sanguinis

1T-277

A. naeslundii, F. nucleatum, P. gingivalis,
IYYMRFVNKPLEKTFFKI
243

T. denticola, S. gordonii,

S. mitis, S. mutans, S. oralis,

S. salivarious, S. sanguinis

1T-278

A. naeslundii, F. nucleatum, P. gingivalis,
SINSSAGIQPHCLSSSFVLR
244

S. gordonii, S. mitis,
TKHCFY

S. mutans, S. oralis, S. salivarious,

S. sanguinis

1T-279

A. naeslundii, F. nucleatum, P. gingivalis,
FVLRTKHCFY
245

S. gordonii, S. mitis,

S. mutans, S. oralis, S. salivarious,

S. sanguinis

1T-280

A. naeslundii, F. nucleatum, P. gingivalis,
TNNKNKVIIKAIKFKNKDF
246

T. denticola, S. gordonii,
INLDLFIYRR

S. mitis, S. mutans, S. oralis,

S. salivarious, S. sanguinis

1T-281

A. naeslundii, F. nucleatum, P. gingivalis,
KYEKLTKENLFIRNSGNM
247

S. epidermidis, S. gordonii,
CVFIYFLFFG

S. mitis, S. mutans, S. oralis,

S. salivarious, S. sanguinis

1T-282

F. nucleatum, P. gingivalis, S. gordonii,
ISLVFPAYT
248

S. mitis, S. mutans, S. oralis,

S. salivarious, S. sanguinis

1T-283

A. naeslundii, F. nucleatum, P. gingivalis,
LCTKLEDKQRGRIPAELFII
249

T. denticola, S. epidermidis,
SPIKILERNDAL

S. gordonii, S. mitis,

S. mutans, S. oralis, S. salivarious,

S. sanguinis

1T-284

A. naeslundii, F. nucleatum, P. gingivalis,
FQYYFSLKRV
250

S. gordonii, S. mitis,

S. mutans, S. oralis, S. salivarious,

S. sanguinis

1T-285

A. naeslundii, F. nucleatum, P. gingivalis,
FFPYYLADFYKQLKFLNE
251

S. gordonii, S. mitis,
YQTKNKDKVVEFK

S. mutans, S. oralis, S. salivarious,

S. sanguinis

1T-286

S. sanguinis

LGFFNNKADLVKADTERD
252

NRMSSLKIKDL

1T-287

P. gingivalis, T. denticola, S. gordonii,
KGYPLPFQYRLNNH
253

S. mitis, S. mutans, S. oralis,

S. salivarious, S. sanguinis

1T-288

F. nucleatum, S. gordonii, S. salivarious,
RWVGGEPSADIYLSAKDT
254

S. sanguinis

KT

1T-289

F. nucleatum, P. gingivalis, S. gordonii,
EPSADIYLSAKDTKT
255

S. mitis, S. mutans, S. oralis,

S. sanguinis

1T-290

A. naeslundii, F. nucleatum, P. gingivalis,
IINQLNLILLRLMEILIL
256

S. gordonii, S. mitis,

S. mutans, S. oralis, S. salivarious,

S. sanguinis

1T-291

A. naeslundii, F. nucleatum, P. gingivalis,
DMKIIKLYIKILSFLFIKYC
257

T. denticola, S. mitis,
NKKLNSVKLKA

S. mutans, S. oralis

1T-292

A. naeslundii, F. nucleatum, P. gingivalis,
IINQLNLILLRLMEILIL
258

S. epidermidis, S. gordonii,

S. mitis, S. mutans, S. oralis,

S. salivarious, S. sanguinis

1T-293

A. naeslundii, F. nucleatum, P. gingivalis,
HVEDCFLLSNARTTAIHG
259

S. epidermidis, S. gordonii,
RANPARGEPRTRSE

S. mitis, S. mutans, S. oralis,

S. salivarious, S. sanguinis

1T-294

T. denticola

YDKIADGVFKIGKRGVL
260

1T-295

S. mitis, S. salivarious, S. sanguinis
KYKLKKIIL
261

1T-296

A. naeslundii, F. nucleatum, P. gingivalis,
EYSQQSFKAKPCSERGVL
262

S. gordonii, S. mitis,
SP

S. mutans, S. oralis, S. salivarious,

S. sanguinis

1T-297

A. naeslundii, F. nucleatum, T. denticola,
RSLRLNNALTKLPKLWYN
263

S. mitis, S. mutans, S. oralis
RIKEAFYAYNDYDK

1T-298

A. naeslundii, F. nucleatum, P. gingivalis,
ILNKKPKLPLWKLGKNYF
264

T. denticola, S. gordonii,
RRFYVLPTFLA

S. mitis, S. mutans, S. oralis,

S. salivarious, S. sanguinis

1T-299

A. naeslundii, F. nucleatum, S. epidermidis,
SMLTSFLRSKNTRSLKMY
265

S. gordonii, S. mitis,
KDVHF

S. mutans, S. oralis, S. salivarious,

S. sanguinis

1T-300

A. naeslundii, F. nucleatum, P. gingivalis,
PLIISKAQIKMSGDILGSCF
266

S. epidermidis, S. gordonii,
KLFYLRPFF

S. mitis, S. mutans, S. oralis,

S. salivarious, S. sanguinis

1T-301

F. nucleatum, S. gordonii, S. sanguinis
SKLPRVLDASLKL
267

1T-302

A. naeslundii, P. gingivalis, S. epidermidis,
IIIILPKIYLVCKTV
268

S. gordonii, S. mitis,

S. mutans, S. oralis, S. salivarious,

S. sanguinis

1T-303

A. naeslundii, F. nucleatum, P. gingivalis,
LDYENMDCKKRIRI
269

S. gordonii, S. mitis,

S. mutans, S. oralis, S. salivarious,

S. sanguinis

1T-304

P. gingivalis

STAGEASRRTASEASRRT
270

AAKLRG

TT-305

F. nucleatum

ARNALNMRDVPVDAAIIG
271

IIDGMDEE

TT-306

F. nucleatum

KILNEAEGKLLKVIEKNGE
272

IDIEEI

TT-307

F. nucleatum

NGDKKAKEELDKWDEVI
273

KELNIQF

TT-308

F. nucleatum

GLVIIPNLIALIILFSQVRQQ
274

TKDYFSNPKLSSR

TT-309

F. nucleatum

EPLPLTKYDKKDTEMKKV
275

FKEILAGKVGYEKEEE

TT-310

F. nucleatum,
TKLKKNNKLLSAKKENTL
276

HTKDK

TT-311

S. mutans, S. sobrinus
AIFDAMHNL
277

PF-060

C. albicans hyphae
HSSHL
278

PF-024

C. albicans hyphae
DLRKAK
279

PF-636

C. albicans hyphae
LVRLA
280

PF-178

C. albicans hyphae
EVYSSPTNNVAITVQNN
281

PF-761

C. albicans hyphae
SKFELVNYASGCSCGADC
282

KCASETECKCASKK

PF-770

C. albicans hyphae
GVGIGFIMMGVVGYAVK
283

LVHIPIRYLIV

1T-65

C. albicans hyphae
HARAAVGVAELPRGAAV
284

EVELIAAVRP

PF-141

C. albicans hyphae
VVRRFQGM
285

PF-543

C. albicans hyphae
NILFGIIGFVVAMTAAVIV
286

TAISIAK

PF-634

C. albicans hyphae
MPKARPVNHNKKKSKITI
287

KSNFTLFYMFNP

PF-040

C. albicans hyphae
MIHLTKQNTMEALHFIKQ
288

FYDMFFILNFNV

PF-051

C. albicans hyphae
RFFNFEIKKSTKVDYVFAH
289

VDLSDV

PF-580

C. albicans hyphae
EILNNNQVIKELTMKYKT
290

QFESNLGGWTARARR

PF-583

C. albicans hyphae
KFQGEFTNIGQSYIVSASH
291

MSTSLNTGK

1T-36

C. albicans hyphae
VYRHLRFIDGKLVEIRLERK
292

PF-206

C. albicans hyphae
KLRSASKKSLQEKSCGIMP
293

EKPAG

1T-13
yeast and hyphal forms
FRSPCINNNSLQPPGVYPAR
294

1T-21
yeast and hyphal forms
YVEEAVRAALKKEARIST
295

EDTPVNLPSFDC

PF-030
yeast and hyphal forms
MTCHQAPTTTHQSNMA
296

PF-463
yeast and hyphal forms
MVILVFSLIFIFTDNYLVY
297

QSKSIKEDVMI

PF-380
yeast and hyphal forms
KKIIPLITLFVVTLVG
298

PF-515
yeast and hyphal forms
DKSTQDKDIKQAKLLAQE
299

LGL-NH2

PF-458
yeast and hyphal forms
ISLIIFIMLFVVALFKCITNY
300

KHQS

PF-S018
yeast and hyphal forms
GMPQIPRLRI
301

1T-16
yeast and hyphal forms
IDMR
302

PF-211
yeast and hyphal forms
DSFDSLSPFRERGGEREDG
303

CDAMPLP

PF-002
yeast and hyphal forms
NDDAQ
304

PF-S003
yeast and hyphal forms
ALALLKQDLLNFEGRGRII
305

TSTYLQFNEGCVP

PF-021
yeast and hyphal forms
FSLNFSKQKYVTVN
306

1T-14
yeast and hyphal forms
ALAGLAGLISGK
307

1T-15
yeast and hyphal forms
DVILRVEAQ
308

PF-629
yeast and hyphal forms
GLAAIATVFALY
309

PF-617
yeast and hyphal forms
PMNAAEPE
310

PF-621
yeast and hyphal forms
PPSSFLV
311

PF-631
yeast and hyphal forms
LIIYFSKTGNTARATRQI
312

PF-009
yeast and hyphal forms
SKKYNHILNQENR
313

1T-17
yeast and hyphal forms
NNAIVYIS
314

1T-20
yeast and hyphal forms
PALVDLSNKEAVWAVLD
315

DHS

1T-68
yeast cells
GGTKEIVYQRG
316

1T-70
yeast cells
NRQAQGERAHGEQQG
317

PF-167
yeast cells
AIEGVIKKGACFKLLRHE
318

MF

PF-168
yeast cells
VLPFPAIPLSRRRACVAAP
319

RPRSRQRAS

PF-170
yeast cells
RLARGRPTNLCGRRG
320

PF-176
yeast cells
RLTSNQFLTRITPFVFAQH
321

Rv2369c

C. albicans

VRQVPVDRPESRHQKPGD
322

PF2-038

VPRDPRC

Rv2561

C. albicans

QHQCPGMRPAPADAPEVP
323

PF2-040

HAARADQKRPSLRL

Rv1535

C. albicans

DPLVDGAARLLSIPLRHLY
324

PF2-033

AALWRVGLLEVQA

Rv2660c

C. albicans

RSPDFVDETAGQSWCAIL
325

PF2-044

GLNQFH

Rv3760

C. albicans

GLITVFAGTARILQLRRAA
326

PF2-059

KKTHAAALR

PF-S024

Corynebacteria spp.
SKRGRKRKDRRKKKANH
327

GKRPNS

PF-001

S. epidermidis, M. luteus, P. mirabilis,
MNNWIIVAQLSVTVINEII
328

E. coli, P. aeruginosa,
DIMKEKQKGGK

C. albicans, MRSA, E. faecalis,

C. jeikeium

PF-002

S. epidermidis, P. mirabilis, C. albicans,
NDDAQ
329

C. jeikeium, C. jejuni

PF-003

S. epidermidis, M. luteus, P. mirabilis,
MNNWIKVAQISVTVINEVI
330

C. albicans, MRSA, C. jeikeium
DIMKEKQNGGK

PF-004

S. epidermidis, B. subtilis, B. fragilis,
ARLSKAIIIAVIVVYHLDV
331

E. coli, P. aeruginosa, C. albicans,
RGLF

S. pneumoniae, E. faecalis,

C. jeikeium

PF-005

S. epidermidis, E. coli, MRSA, S. pneumoniae,
MESIFKIKLMNGICRSENM
332

E. faecalis

NMKKKNKGEKI

PF-006

S. epidermidis, M. luteus, E. coli,
MGIIAGIIKFIKGLIEKFTGK
333

P. aeruginosa, MRSA, E. faecalis,

C. jeikeium, C. jejuni

PF-007

S. epidermidis, M. luteus, E. coli,
MGIIAGIIKVIKSLIEQFTGK
334

P. aeruginosa, C. albicans,

MRSA, S. pneumoniae, E. faecalis,

C. jeikeium

PF-008

S. epidermidis, M. luteus, MRSA,
MIEIGSIAYLNGGSKKYNH
335

C. jejuni

ILNQENR

PF-009

M. luteus, P. mirabilis, C. albicans
SKKYNHILNQENR
336

PF-010

S. epidermidis, M. luteus, E. coli,
MDIDVNKLLQAFVYFKSF
337

C. albicans

EKLRHNNS

PF-011

M. luteus, E. coli, P. aeruginosa,
MFCYYKQHKGDNFSIEEV
338

S. pneumoniae, C. jeikeium
KNIIADNEMKVN

PF-012

S. epidermidis, M. luteus, P. mirabilis,
WRGPNTEAGGKSANNIVQ
339

E. coli, P. aeruginosa,
VGGAPT

MRSA, S. pneumoniae, C. jeikeium,

C. jejuni

PF-013

M. luteus, P. mirabilis, E. coli, P. aeruginosa,
LIQKGLNQTFIVVIRLNNFI
340

MRSA, S. pneumoniae,
KKS

C. jeikeium, C. jejuni

PF-014

E. coli, C. jeikeium
HPTDNKHN
341

PF-015

E. faecalis, C. jeikeium
SIDKRNLYNLKYYE
342

PF-016

S. epidermidis, E. faecalis, C. jeikeium
RKQYDDLSFNFLY
343

PF-017

E. coli

ESIIE
344

PF-018

E. coli, C. jeikeium
YYKTYFKEV
345

PF-020

S. epidermidis, M. luteus, C. albicans,
MKIILLLFLIFGFIVVVTLK
346

MRSA, S. pneumoniae,
SEHQLTLFSI

E. faecalis

PF-021

S. epidermidis, M. luteus, P. mirabilis,
FSLNFSKQKYVTVN
347

E. coli, C. albicans, E. faecalis,

C. jeikeium

PF-022

M. luteus, P. mirabilis, E. coli, P. aeruginosa,
MINELKNKNSGIMNNYVV
348

C. albicans, MRSA,
TKESKL

S. pneumoniae, E. faecalis, C. jeikeium,

C. jejuni

PF-023

M. luteus, C. jeikeium
MTKNTIISLENEKTQINDS
349

ENESSDLRKAK

PF-024

M. luteus, C. albicans, MRSA, E. faecalis,
DLRKAK
350

C. jeikeium

PF-025

S. epidermidis, M. luteus, P. mirabilis,
LLIIFRLWLELKWKNKK
351

E. coli, P. aeruginosa,

MRSA, E. faecalis, C. jejuni

PF-026

S. epidermidis, M. luteus, P. mirabilis,
SIHFIN
352

C. albicans, MRSA, E. faecalis,

C. jeikeium

PF-027

M. luteus, MRSA, E. faecalis, C. jejuni
HNARKYLEFISQKIDGDKL
353

TKEDSL

PF-028

S. epidermidis, M. luteus, MRSA
ALDCSEQSVILWYETILDK
354

IVGVIK

PF-029

S. epidermidis, M. luteus, C. albicans,
NSTNE
355

C. jejuni

PF-030

S. epidermidis, M. luteus, P. mirabilis,
MTCHQAPTTTHQSNMA
356

E. coli, P. aeruginosa,

C. albicans, MRSA, S. pneumoniae,

E. faecalis, C. jeikeium,

C. jejuni

PF-031

M. luteus, C. albicans
MPHHSTTSSRIVVPAHQS
357

NMASTPNLSITP

PF-033

S. epidermidis, M. luteus, E. coli,
MFIFKTTSKSHFHNNVKSL
358

P. aeruginosa, C. albicans,
ECIKIPINKNR

MRSA, S. pneumoniae

PF-034

M. luteus

EPKKKHFPKMESASSEP
359

PF-035

S. epidermidis, M. luteus, E. coli,
SFYESY
360

C. albicans, MRSA, C. jeikeium,

C. jejuni

PF-036

S. epidermidis, M. luteus, P. mirabilis,
ILNRLSRIVSNEVTSLIYSLK
361

E. coli, C. albicans,

MRSA, S. pneumoniae, C. jejuni

PF-037

S. epidermidis, M. luteus, P. aeruginosa,
MTKKRRYDTTEFGLAHS
362

C. albicans, MRSA,
MTAKITLHQALYK

S. pneumoniae, E. faecalis, C. jeikeium

PF-038

M. luteus

MAYKDEGKETKFAVKGY
363

KD

PF-039

P. mirabilis, C. jeikeium
MLEEKNKSL
364

PF-040

S. epidermidis, M. luteus, P. mirabilis,
MIHLTKQNTMEALHFIKQ
365

E. coli, P. aeruginosa,
FYDMFFILNFNV

C. albicans, MRSA, S. pneumoniae,

E. faecalis, C. jeikeium,

C. jejuni

PF-041
MRSA
ELLVILPGFI
366

PF-042

S. epidermidis, M. luteus, P. mirabilis,
LLLSYFRYTGALLQSLF
367

E. coli, P. aeruginosa,

C. albicans, MRSA, S. pneumoniae,

E. faecalis, C. jeikeium

PF-043

M. luteus, C. jejuni
MIKNETAYQMNELLVIRS
368

AYAK

PF-044

S. epidermidis, M. luteus, MRSA,
KLKKYIHKPD
369

C. jeikeium

PF-045

S. epidermidis, E. coli, E. faecalis,
LDINDYRSTY
370

C. jejuni

PF-046

E. coli, E. faecalis, C. jeikeium
LDFYLTKHLTLML
371

PF-047

S. mutans

NQEPSLQQDKEQKDNKG
372

PF-048

S. epidermidis, M. luteus, E. coli,
LYFAFKKYQERVNQAPNI
373

MRSA, C. jeikeium, C. jejuni
EY

PF-049

S. epidermidis, MRSA, C. jeikeium,
AYYLKRREEKGK
374

C. jejuni

PF-050

S. epidermidis, M. luteus, E. coli,
SYYLKRREEKGK
375

C. jeikeium

PF-051

S. epidermidis, M. luteus, P. aeruginosa,
RFFNFEIKKSTKVDYVFAH
376

C. albicans, MRSA,
VDLSDV

S. pneumoniae, E. faecalis

PF-052

S. epidermidis, M. luteus, E. coli,
QELINEAVNLLVKSK
377

MRSA, E. faecalis, C. jeikeium,

C. jejuni

PF-053

S. epidermidis, M. luteus, E. coli,
KLFGQWGPELGSIYILPAL
378

P. aeruginosa, C. albicans,
IGSIILIAIVTLILRAMRK

MRSA, S. pneumoniae, E. faecalis

PF-054

S. epidermidis, E. coli
VSISRFIGGGHVFNGNNKR
379

NL

PF-055

S. mutans

GHVFNGNNKRNL
380

PF-056

S. epidermidis, M. luteus, P. mirabilis,
AEQLFGKQKQRGVDLFLN
381

E. coli, P. aeruginosa,
RLTIILSILFFVLMICISYLGM

C. albicans, MRSA, S. pneumoniae,

E. faecalis, C. jeikeium

PF-057

S. epidermidis, M. luteus, P. mirabilis,
TMIVISIPRFEEYMKARHK
382

E. coli, P. aeruginosa,
KWM

C. albicans, MRSA, S. pneumoniae,

E. faecalis, C. jeikeium,

C. jejuni

PF-058

S. epidermidis, M. luteus, E. coli,
FADQSQDNA
383

C. albicans, MRSA, C. jeikeium,

C. jejuni

PF-060

E. coli, C. albicans, C. jeikeium
HSSHL
384

PF-061

S. epidermidis, S. pneumoniae
GYNSYKAVQDVKTHSEE
385

QRVTAKK

PF-062

S. epidermidis, M. luteus, E. coli,
MKKKRINNDILGRMIYSSS
386

P. aeruginosa, MRSA, S. pneumoniae,
IDKRNLYNLKYYE

E. faecalis, C. jeikeium

PF-063

S. epidermidis, M. luteus, E. coli,
IAAIIVLVLFQKGLLQIFN
387

P. aeruginosa, MRSA, S. pneumoniae,
WILIQLQ

E. faecalis, C. jeikeium,

C. jejuni

PF-064

E. coli

DYYGKE
388

PF-065

M. luteus, E. coli, P. aeruginosa,
LEKNTRDNYFIHAIDRIYI
389

C. albicans, MRSA, S. pneumoniae,
NTSKGLFPESELVAWG

C. jeikeium, C. jejuni

PF-066

M. luteus, E. coli, C. jeikeium
IKGTVKAVDETTVVITVN
390

GHGTELTFEKPAIKQVDPS

PF-067

S. epidermidis, M. luteus, P. mirabilis,
DLIVKVHICFVVKTASGY
391

E. coli, P. aeruginosa,
CYLNKREAQAAI

C. albicans, MRSA, S. pneumoniae,

E. faecalis, C. jeikeium,

C. jejuni

PF-068

S. epidermidis, M. luteus, P. mirabilis,
SHLINNFGLSVINPSTPICL
392

E. coli, P. aeruginosa,
NFSPVFNLLTVYGITCN

C. albicans, MRSA, S. pneumoniae,

E. faecalis, C. jeikeium

PF-069

E. faecalis, C. jejuni
FDPVPLKKDKSASKHSHK
393

HNH

PF-070

S. epidermidis, C. jejuni
SMVKSEIVDLLNGEDNDD
394

PF-071

S. epidermidis, E. coli, P. aeruginosa,
HCVIGNVVDIANLLKRRA
395

C. albicans, MRSA,
VYRDIADVIKMR

S. pneumoniae, E. faecalis, C. jeikeium,

C. jejuni

PF-073

S. epidermidis, M. luteus, P. aeruginosa,
CPSVTMDACALLQKFDFC
396

C. albicans, MRSA,
NNISHFRHFFAIKQPIER

S. pneumoniae, E. faecalis

PF-074

S. epidermidis, M. luteus, MRSA
RDIHPIYFMTKD
397

PF-075

M. luteus, E. coli, P. aeruginosa,
FVNSLIMKDLSDNDMRFK
398

MRSA, C. jeikeium
YEYYNREKDT

PF-076

S. epidermidis, M. luteus, E. coli,
LYQYELLSKEEYLKCTLII
399

P. aeruginosa, C. albicans,
NQRRNEQK

MRSA, S. pneumoniae, E. faecalis,

C. jeikeium, C. jejuni

PF-097

C. jeikeium

QPTQGEQGTRPRRPTPMR
400

GLLI

PF-099

S. epidermidis, M. luteus, E. coli,
EIIAYLEGRFANA
401

C. jeikeium

PF-101

S. mutans

DPVPERQEQACACHRTAK
402

PGK

PF-104
MRSA, C. jeikeium
ERTAVNDLWI
403

PF-123

M. luteus, E. coli
TTRPQVAEDRQLDDALKE
404

TFPASDPISP

PF-124

S. epidermidis, M. luteus, P. mirabilis,
MADGQIAAIAKLHGVPVA
405

E. coli, P. aeruginosa,
TRNIRHFQSFGVELINPWSG

C. albicans, MRSA, E. faecalis,

C. jejuni

PF-125

S. epidermidis, M. luteus, P. mirabilis,
YVVGALVILAVAGLIYSM
406

E. coli, P. aeruginosa,
LRKA

C. albicans, MRSA, S. pneumoniae,

E. faecalis, C. jejuni

PF-126

S. epidermidis, M. luteus, P. mirabilis,
FSPEAFGIGAAGVLGSFVT
407

E. coli, P. aeruginosa,
GLLIGWVASLLRKAK

C. albicans, MRSA, S. pneumoniae,

E. faecalis, C. jeikeium,

C. jejuni

PF-127

S. epidermidis, M. luteus, E. coli,
MLRYLSLFAVGLATGYA
408

P. aeruginosa, C. albicans,
WGWIDGLAASLAV

MRSA, S. pneumoniae, E. faecalis

PF-128

M. luteus, P. aeruginosa, E. faecalis
GIKVVAARFEEIQFSENFD
409

SIILA

PF-129

S. epidermidis, MRSA, E. faecalis,
MKLLARDPWVCAWNDIW
410

C. jeikeium, C. jejuni

PF-130

E. faecalis, C. jeikeium, C. jejuni
LQRSDEESMPRRHEKYS
411

PF-131

S. epidermidis, E. coli, MRSA, C. jeikeium
RRAAARTKGNRR
412

PF-132

S. epidermidis, C. jeikeium
RPGDGAAEQGRSR
413

PF-133

S. epidermidis, C. jeikeium, C. jejuni,
GDPTAGQKPVECP
414

M. smegmatis

PF-134

S. epidermidis, C. jeikeium
GKAMKRQDCSAL
415

PF-135

S. epidermidis, M. luteus, E. coli,
PPARPARIPQTPTLHGASL
416

P. aeruginosa, MRSA, C. jeikeium,
FRQRS

M. smegmatis

PF-136

S. epidermidis, M. luteus, P. mirabilis,
LRGRVGRITACGYPP
417

E. coli, MRSA, E. faecalis,

C. jeikeium, C. jejuni, M. smegmatis

PF-137

S. epidermidis, P. mirabilis, S. pneumoniae,
VLGKGHDLLDVGKTALK
418

C. jeikeium, C. jejuni
SRVFAWLGGS

PF-138

S. epidermidis, M. luteus, P. mirabilis,
AVHHSLLFR
419

E. coli, C. albicans,

MRSA, C. jeikeium, C. jejuni

PF-139

S. epidermidis, M. luteus, P. mirabilis,
ALSKPAIQARTLCRRQDPP
420

E. coli, P. aeruginosa,

C. albicans, S. pneumoniae, E. faecalis,

C. jeikeium, C. jejuni

PF-140

S. epidermidis, M. luteus, P. mirabilis,
FHRRVIRASEWALTTRSFS
421

E. coli, P. aeruginosa,
TPLRSAAR

C. albicans, MRSA, S. pneumoniae,

E. faecalis, C. jeikeium,

C. jejuni, M. smegmatis

PF-141

S. epidermidis, M. luteus, C. albicans,
VVRRFQGM
422

MRSA, C. jeikeium

PF-142

S. mutans

GIDRGCQAAR
423

PF-143

S. epidermidis, MRSA, C. jeikeium
LSPRPIIVSRRSRADNNND
424

WSR

PF-144

S. epidermidis, M. luteus, E. coli,
RSGQPVGRPSRRAWLR
425

C. albicans, MRSA, S. pneumoniae,

E. faecalis, C. jeikeium

PF-145

S. epidermidis, M. luteus, P. mirabilis,
GIVLTGRAGLVSGACSMA
426

E. coli, P. aeruginosa,
LGVGLG

C. albicans, MRSA, S. pneumoniae,

E. faecalis, C. jeikeium,

C. jejuni, M. smegmatis

PF-146

S. epidermidis, M. luteus, P. aeruginosa,
GCGKRRIITKSASRDTR
427

C. albicans, MRSA,

C. jeikeium

PF-147

S. epidermidis, M. luteus, MRSA
RRPRRRRSGHGQSASAA
428

PF-148

S. epidermidis, M. luteus, P. mirabilis,
RRGCTERLRRMARRNAW
429

E. coli, P. aeruginosa,
DLYAEHFY

C. albicans, MRSA, S. pneumoniae,

E. faecalis, C. jeikeium,

C. jejuni, M. smegmatis

PF-149

S. epidermidis, M. luteus, E. coli,
GKVSVLTRVPRSLGGAPA
430

MRSA, C. jeikeium
NQ

PF-150

S. epidermidis, MRSA
EIQAKGTG
431

PF-151

S. epidermidis, MRSA, E. faecalis,
EEYPARVPLSGEDVTEAR
432

C. jeikeium

RH

PF-152

S. epidermidis, C. albicans,
VGYFIWKDSHSRKG
433

MRSA, E. faecalis, C. jeikeium

PF-153

M. luteus, P. mirabilis, E. coli,
GILARADCSQIA
434

MRSA

PF-154

S. mutans

GIKKSKHPSTDDYVVKTTI
435

DSL

PF-155

C. jeikeium

GRYGDDSKERQGRAQ
436

PF-156

S. epidermidis, C. jeikeium
LITAEQPATAPIAGK
437

PF-157

S. epidermidis, M. luteus, P. mirabilis,
HTAVVWLAGVSGCVALS
438

E. coli, P. aeruginosa,
HCEPA

C. albicans, MRSA, S. pneumoniae,

E. faecalis, C. jeikeium,

C. jejuni

PF-158

S. epidermidis

VRLESRPADLPE
439

PF-159

S. epidermidis

TMAFVEKAQLRVPVGDD
440

LPV

PF-160

S. epidermidis

SFHASLTKNEKPIKSTG
441

PF-161

S. epidermidis, M. luteus, E. coli,
RGRALASTATTRPARRRR
442

C. jejuni

PF-162

S. epidermidis, MRSA
GIRRLHSVENLNREISHRM
443

AGLR

PF-163

S. epidermidis

TSWLRAAERQEIGEPTKTF
444

GEKTTSL

PF-164

S. epidermidis, M. luteus, E. coli,
EEVSRALAGIGLGLGCRIG
445

C. jeikeium

PF-165
MRSA, C. jejuni
GPVSVVASLRRGTTVQRH
446

SQNNHNKGKP

PF-166

E. coli, C. jeikeium
SKAVSRKRSI
447

PF-167

S. epidermidis, E. coli, C. albicans,
AIEGVIKKGACFKLLRHE
448

MRSA, C. jeikeium, C. jejuni
MF

PF-168

S. epidermidis, M. luteus, E. coli,
VLPFPAIPLSRRRACVAAP
449

C. albicans, MRSA, C. jeikeium,
RPRSRQRAS

C. jejuni

PF-169

S. epidermidis, E. coli, C. albicans,
APGSAADSPRSRADD
450

E. faecalis, C. jeikeium

PF-170

S. epidermidis, M. luteus, P. mirabilis,
RLARGRPTNLCGRRG
451

E. coli, P. aeruginosa,

C. albicans, MRSA, S. pneumoniae,

E. faecalis, C. jejuni

PF-171

S. epidermidis, E. coli, P. aeruginosa,
TQVTLCRTW
452

S. pneumoniae

PF-172

S. epidermidis, M. luteus, E. coli,
LTGVRRPWRAPWAGTSG
453

P. aeruginosa, MRSA, E. faecalis,
WALR

C. jejuni

PF-173

S. epidermidis, M. luteus, P. mirabilis,
AGRTAIVQGGG
454

E. coli, P. aeruginosa,

C. albicans, C. jeikeium, C. jejuni

PF-174

S. epidermidis, P. aeruginosa, C. jeikeium
RGGDSPARRRPGLAGPGG
455

PG

PF-175

S. epidermidis, E. faecalis
RRRPAGQRPEKASQAMIAA
456

PF-176

S. epidermidis, M. luteus, P. mirabilis,
RLTSNQFLTRITPFVFAQH
457

E. coli, C. albicans,

MRSA, E. faecalis, C. jeikeium

PF-177

M. luteus, MRSA, E. faecalis, C. jeikeium
VTSEPGIAHDIRLLPRAAA
458

FR

PF-178

S. epidermidis, M. luteus, B. subtilis,
EVYSSPTNNVAITVQNN
459

P. mirabilis, E. coli, P. aeruginosa,

C. albicans, MRSA,

S. pneumoniae, E. faecalis, C. jeikeium

PF-180

S. epidermidis, M. luteus, P. aeruginosa,
SGLGDLGFSSEAK
460

C. albicans, MRSA,

E. faecalis, C. jejuni, M. smegmatis

PF-181

S. epidermidis, M. luteus, E. coli,
GIAPRRNEWGAVGGR
461

MRSA, E. faecalis, C. jeikeium

PF-182

S. epidermidis, M. luteus, E. coli,
LPATRDKTRVPASVAGAP
462

E. faecalis, C. jeikeium

PF-183

S. epidermidis, M. luteus, E. coli,
KPGISVENRQ
463

C. albicans, MRSA, E. faecalis,

C. jeikeium

PF-184

S. epidermidis, M. luteus, E. coli,
LIADRHIRA
464

P. aeruginosa, C. albicans,

MRSA, C. jeikeium

PF-185

E. coli, P. aeruginosa
RPAQARQGPGGLIADRHI
465

RA

PF-186

S. epidermidis, M. luteus, E. coli,
DADKNLSLERDRFAWRV
466

P. aeruginosa, MRSA, C. jeikeium
AAP

PF-187

S. epidermidis, M. luteus, E. coli,
EIQKIAKGVSGQVYGPSR
467

MRSA
QITISKKR

PF-188

S. epidermidis, M. luteus, E. coli,
ARTFAGRLGTRYFGGLMR
468

C. albicans, MRSA, E. faecalis
STKA

PF-189

S. epidermidis, M. luteus, C. albicans,
GNLTRSREAARATQ
469

MRSA, E. faecalis, C. jejuni

PF-190

S. epidermidis, M. luteus, P. mirabilis,
HFILRKPLLFMIHSLKTGP
470

E. coli, P. aeruginosa,
LDRF

C. albicans, MRSA, S. pneumoniae,

E. faecalis, C. jeikeium

PF-191

E. coli, P. aeruginosa, C. jejuni
QFCNFAWLFLASNNAQVS
471

ALA

PF-192

S. epidermidis, M. luteus, P. aeruginosa,
VEEDEAPPPHY
472

C. albicans, E. faecalis,

C. jeikeium

PF-193

S. epidermidis, M. luteus, E. coli,
PPHCPPGHAKKGWC
473

MRSA, E. faecalis, C. jejuni

PF-194

C. jeikeium

MKGNKLATAHEQPVKNS
474

APPL

PF-195

S. epidermidis, M. luteus, E. faecalis,
EMAEGSADDRLRKTPRDC
475

C. jeikeium

PF-196

S. epidermidis, M. luteus, P. mirabilis,
TTARYIRRQCHTSITPLSQG
476

E. coli, P. aeruginosa,

C. albicans, MRSA, S. pneumoniae,

E. faecalis, C. jejuni

PF-197

S. epidermidis, M. luteus, C. albicans,
CNALLRRGHPPSAL
477

E. faecalis, C. jejuni

PF-200

S. epidermidis, M. luteus, MRSA,
GIELKSLIMAQIERWRQA
478

E. faecalis, C. jeikeium

PF-201

S. epidermidis, M. luteus, E. coli,
GCRPASLSDADPDGR
479

C. albicans, E. faecalis, C. jeikeium,

C. jejuni

PF-202

S. epidermidis, M. luteus, E. coli,
ALNRASLRLALGE
480

MRSA, E. faecalis, C. jeikeium,

C. jejuni

PF-203

S. epidermidis, M. luteus, P. mirabilis,
SWKCHHLAI
481

E. coli, P. aeruginosa,

C. albicans, MRSA, S. pneumoniae,

E. faecalis, C. jejuni

PF-204

S. epidermidis, P. mirabilis, E. coli,
ALQKQDMNLPSVKNQLV
482

P. aeruginosa, C. albicans,
FLKSTG

C. jejuni

PF-205

S. epidermidis, M. luteus, E. coli,
AGVLETPRCRGEYGAN
483

P. aeruginosa, C. albicans,

MRSA, E. faecalis, C. jeikeium,

C. jejuni

PF-206

M. luteus, C. albicans, C. jeikeium,
KLRSASKKSLQEKSCGIMP
484

C. jejuni

EKPAG

PF-207

M. luteus, C. jeikeium
AAGCRDLGSLSSLVTNPS
485

PF-208

S. epidermidis, C. albicans,
DAYHCHLVRSPDAHDLS
486

MRSA, S. pneumoniae, E. faecalis,
MRIGFV

C. jeikeium, C. jejuni

PF-209

C. albicans, C. jeikeium, C. jejuni
NYAVVSHT
487

PF-210

S. epidermidis, P. aeruginosa, C. albicans,
EREDGCDAMPLP
488

MRSA, E. faecalis, C. jeikeium,

C. jejuni

PF-211

S. epidermidis, M. luteus, E. coli,
DSFDSLSPFRERGGEREDG
489

P. aeruginosa, C. albicans, S. pneumoniae,
CDAMPLP

E. faecalis, C. jeikeium,

C. jejuni, M. smegmatis

PF-212

M. luteus, P. aeruginosa
NDSKASN
490

PF-213

S. epidermidis

MTTGVDFIIEKV
491

PF-214

S. mutans, S. epidermidis, M. luteus,
GHLRVCWVFSASLLTPFR
492

E. coli, P. aeruginosa, A. baumannii
SATLI

PF-215

S. epidermidis, M. luteus, P. aeruginosa,
ELKITNYNVNTVLYRYYK
493

A. baumannii

WGNDLCE

PF-216

S. mutans, E. coli
ESVDKITEALEEDGFPAKVQ
494

PF-217

S. mutans

DWEFTHKTIPQKK
495

PF-218

S. epidermidis, M. luteus, P. aeruginosa,
SETPEKPVGTFFYSIYYKIIL
496

A. baumannii

PF-219

S. epidermidis, M. luteus, P. aeruginosa,
FLALAVIAGLFKVILIYAA
497

A. baumannii

PYLK

PF-221

S. epidermidis, M. luteus, P. aeruginosa
VFDNIDINF
498

PF-222

S. epidermidis

HIKETR
499

PF-223

S. epidermidis, M. luteus, A. baumannii
VKFCIECQTKLERKRR
500

PF-224

S. epidermidis, P. aeruginosa, A. baumannii
DYFYITLSQKNTF
501

PF-225

S. epidermidis

MNCASPEFKKLMELYK
502

PF-226

A. baumannii

LMFFSENMDKRDTLSGKF
503

RYFAGSKVIKLMNWLSEN

GK

PF-228

S. mutans

NQLGSQAFAQL
504

PF-229

S. epidermidis, M. luteus, P. aeruginosa,
DPILIQIGFTRFALRKAEAE
505

A. baumannii

KIEIQVEEGVPA

PF-230

S. mutans

EDKPTNTIQEIKPVKWQ
506

PF-231

S. mutans

AVRDFKKSVREEDEAASL
507

NSPRTIDAQVKTSESTSVKS

PF-232

S. epidermidis, M. luteus
FDQLYALEREGKLDELLA
508

PF-233

S. epidermidis, M. luteus, P. aeruginosa,
DANAMARTTIAIVYILALI
509

A. baumannii

ALTISYSL

PF-234

S. epidermidis, M. luteus
RTPYILRS
510

PF-235

S. epidermidis, M. luteus
GIPFSKPHKRQVNYMKSD
511

VLAYIEQNKMAHTA

PF-236

S. mutans, S. epidermidis, E. coli,
KEIRTATVAELNAKRRLTS
512

C. albicans, S. pneumoniae, E. faecalis
AEQALAEVS

PF-237

S. epidermidis

YVKPKVGVHE
513

PF-238

S. mutans, S. epidermidis, E. coli,
RNAVVVTEATFPKYEEEIT
514

P. aeruginosa, C. albicans,
NYLNRRFGEDWSLKLEKC

MRSA, S. pneumoniae, E. faecalis
SVA

PF-239

S. mutans, E. coli
PKHNVVTGVSVDLDYKP
515

PF-240

S. mutans, E. coli
RITEVPPDEHSDR
516

PF-242

S. mutans, E. coli
KLFEDPLIKSKAVENFQTT
517

WHEQCLAKELAKNM

PF-244

S. epidermidis, M. luteus, P. aeruginosa,
HMRTISYLLAFAKFSLFIPP
518

A. baumannii

KQSLKRL

PF-245

S. epidermidis, M. luteus, P. aeruginosa,
MNDVKPVVQPKQTLKAF
519

A. baumannii

LVQLLSVRAGVYIKQNNQ

LPKTKG

PF-246

S. mutans

QPDEKAEFFDPSLDKVYR
520

HPTFYHIPDGIEHM

PF-247

S. epidermidis

ETAASETH
521

PF-248

S. mutans, S. epidermidis, M. luteus,
ILSKLWFWMINSLGVVLL
522

E. coli, P. aeruginosa, A. baumannii
VSYWLLAKWGVA

PF-249

S. epidermidis, M. luteus
INSRYKISF
523

PF-252

S. mutans

MKKLVAALAVIVILTGCV
524

YDPVNYDKIHDQEFQDHL

RQNG

PF-253

S. epidermidis, M. luteus
VRDDDS
525

PF-254

S. epidermidis, M. luteus, P. aeruginosa,
FIYGVGFVPHFWLWKWLF
526

A. baumannii

SPWIAWPLMLLGYYIWFLT

PF-255

P. aeruginosa

DHKINESQHNPFRSDSNK
527

QNVDFF

PF-256

S. epidermidis

EYFKQVYVKNEKIYSFWI
528

CKDLSPKEAAKRAEDILV

KLK

PF-257

S. epidermidis

VWENRKKYLENEIERHNV
529

FLKLGQEVIKGLNALASR

GR

PF-259

S. epidermidis, P. aeruginosa, A. baumannii
LPFSKIGRRVSYKKKDVL
530

KYEQSKTVLNTAQLATV

PF-262

S. mutans, S. epidermidis, M. luteus,
DPHSEIDVTRYCQLHHFTC
531

E. coli, P. aeruginosa, A. baumannii
QTMQISEREFHYLIETQ

PF-263

S. epidermidis, M. luteus, A. baumannii
NLKKCPC
532

PF-265

S. epidermidis, M. luteus, A. baumannii
MKTLFFPLFLIIFVLIIQAL
533

DQSYQKKIGISKPQKHPEF

MQ

PF-266

S. mutans

DQEKKNKTEESTEQ
534

PF-267

M. luteus

SDDKRTD
535

PF-268

S. mutans

EVLLSDLRPDIFSET
536

PF-270

S. epidermidis, M. luteus, P. aeruginosa
MYLTPYAWIAVGSIFAFS
537

VTTIKIGDQNDEKQKSHK

NDVHKR

PF-271

S. epidermidis, M. luteus, P. aeruginosa,
AAQPQTTSP
538

A. baumannii

PF-273

S. epidermidis, M. luteus, P. aeruginosa,
LVGALLIFVALIYMVLKG
539

A. baumannii

NADKN

PF-275

S. mutans

LVSGVANTVKNTAHTVG
540

NTAKHAGHVAADTTVKA

TKKQQVK

PF-276

S. epidermidis

LDLALSTNSLNLEGFSF
541

PF-278

M. luteus, A. baumannii
LSLATFAKIFMTRSNWSL
542

KRFNRL

PF-279

S. mutans, S. epidermidis, M. luteus,
SHIGFISISACLAVLLGIAR
543

E. coli, P. aeruginosa, A. baumannii
LFVWTWVKFFA

PF-281

S. mutans, E. coli
SYNTYYNKLIHGQRTPDGM
544

PF-282

S. mutans

QNNDTSAWCGSAHKNGNS
545

PF-283

B. subtilis, B. fragilis, C. difficile
MIRIRSPTKKKLNRNSISD
546

WKSNTSGRFFY

PF-284

C. difficile

MRYITYSLIPRLLSKKVIH
547

QQ

PF-285

S. mutans

VPAKLLRVIDEIPE
548

PF-288

S. mutans, E. coli
IYQLLNIEYSEDD
549

PF-289

C. difficile

MGRHLWNPSYFVATVSE
550

NTEEQIRKYRKNK

PF-291

S. mutans, E. coli
DVDGAIESEL
551

PF-292

S. epidermidis, B. subtilis, B. fragilis
SFVSTTVRLIFEESKRYKF
552

PF-294

S. epidermidis, C. difficile
DFLVNFLWFKGELNWGK
553

KRYK

PF-295

C. difficile

NIQVYESECGNYIFKKSDE
554

SFLIDIFDKNGTH

PF-297

S. epidermidis, B. subtilis, B. fragilis
ISKGIDDIVYVINKILSIGNI
555

FKIIKRK

PF-299

B. subtilis

LATKLKYEKEHKKM
556

PF-300

B. subtilis, C. difficile
VKDVLLELFNKIIGA
557

PF-301

C. difficile

GIVLIGLKLIPLLANVLN
558

PF-304

S. mutans

LVKDTSDIKNDLNNIEIVT
559

SKNSNDIAKLKSVK

PF-305

C. difficile

MREWICPSCNETHDRDIN
560

ASINILKEGLRLITIQNK

PF-306

C. difficile

GCILPHKKDNYNYIMSKF
561

QDLVKITSKK

PF-307

S. epidermidis, B. subtilis, B. fragilis,
MKRRRCNWCGKLFYLEE
562

C. difficile

KSKEAYCCKECRKKAKK

VKK

PF-308

C. difficile

QQYLILDRM
563

PF-309

S. mutans, E. coli
GIPGMTAAPAEENEQEEN
564

ADEE

PF-311

C. difficile

IDAVTKKKTTCMIRAPTKI
565

PIAHTDN

PF-313

S. epidermidis, C. difficile
YITSHKNARAIIKKFERDEI
566

LEEVITHYLNRK

PF-314

S. mutans

ECLKKAIKSKALNKAFKID
567

VPDEVYDNLLMELEEYEK

PF-317

S. mutans

LILVSDI
568

PF-319

S. epidermidis, B. subtilis, C. difficile
SIGSMIGMYSFRHKTKHIK
569

FTFGIPFILFLQFLLVYFYILK

PF-320

S. mutans, E. coli
DSGYYALLENKEERVVW
570

DGEVVANNIFNNLWIVVN

KVKTG

PF-323

S. mutans

ARESIEKSHVPVDATIVGV
571

VDSFEVFDE

PF-324

C. difficile

HFSLL
572

PF-325

S. mutans, E. coli
LTIDEKLRNHR
573

PF-326

S. mutans, E. coli
VIVGNLGAQKEKRNDTPIS
574

AKKDIMGDKTVRVRADL

HH

PF-328

S. mutans

NGNEKAFSEVENLVK
575

PF-329

S. epidermidis

IGILFDKSVRKY
576

PF-333

S. mutans

YMTKKLVEMAEQQMAG
577

KSNR

PF-334

S. epidermidis, C. difficile
QQYLILDRM
578

PF-336

S. mutans, E. coli
MLTSRKKRLKKIVEEQNK
579

KDESI

PF-337

S. epidermidis

YMTKKLVEMAERQMAGK
580

PF-338

S. mutans

KGTSCPDQLSKAIRQSI
581

PF-340

S. mutans, E. coli
VKDVLLELFNKIIGA
582

PF-344

B. subtilis, C. jejuni
DERLPEAKAIRNFNGSVM
583

VLGR

PF-347

S. epidermidis, B. subtilis, B. fragilis,
GIFTGVTVVVSLKHC
584

E. coli, P. aeruginosa, C. albicans,

MRSA, S. pneumoniae,

E. faecalis

PF-348

B. subtilis, E. coli, P. aeruginosa,
ESASAAEWYNPNMNVKK
585

C. albicans, E. faecalis, C. jejuni
AICMG

PF-349

S. epidermidis, B. subtilis, B. fragilis,
MPKSCHVPVLCDFFFLVII
586

E. coli, P. aeruginosa, C. albicans,
KFLALFKTIQS

MRSA, S. pneumoniae,

E. faecalis, C. jeikeium

PF-350

S. epidermidis, E. coli, E. faecalis,
LAVILRAIVY
587

C. jeikeium, C. jejuni

PF-351

S. mutans

YLFFKGKKVAEEEATKDE
588

VKR

PF-352

C. jeikeium

RVKKIG
589

PF-353

S. epidermidis, M. luteus, B. subtilis,
EKTNFKGVKRNFYKKASF
590

E. coli, C. albicans, S. pneumoniae,
FV

E. faecalis, C. jeikeium,

C. jejuni

PF-354

S. epidermidis, B. subtilis, E. coli,
FTFSKCRASNGRGFGTLWL
591

P. aeruginosa, C. albicans,

MRSA, S. pneumoniae, E. faecalis,

C. jeikeium, C. jejuni

PF-355

S. epidermidis, B. subtilis, B. fragilis,
WIAIGLLLYFSLKNQ
592

E. coli, P. aeruginosa, C. albicans,

MRSA, S. pneumoniae,

E. faecalis, C. jeikeium

PF-356

S. epidermidis, B. subtilis, B. fragilis,
VSIKIGAIVIGMIGLMELLTE
593

E. coli, P. aeruginosa, C. albicans,

MRSA, S. pneumoniae,

E. faecalis, C. jeikeium

PF-357

S. epidermidis, M. luteus, P. mirabilis,
MLTIIIGFIFWTMTLMLGY
594

E. coli, P. aeruginosa,
LIGEREGRKHE

C. albicans, MRSA, S. pneumoniae,

E. faecalis, C. jeikeium

PF-358

S. epidermidis, B. subtilis, E. coli,
RNTAHNIKWRSKN
595

C. albicans, MRSA, S. pneumoniae,

E. faecalis, C. jeikeium,

C. jejuni

PF-359

S. epidermidis, B. fragilis, P. aeruginosa,
MTVMEDPGSEQRNKIQSP
596

C. albicans, MRSA,
MKGEDFSALFGR

E. faecalis, C. jeikeium

PF-360

S. epidermidis, B. subtilis, E. coli,
MEQKVKVIFVPRSKPDNQ
597

P. aeruginosa, C. albicans, E. faecalis,
LKTFVSAVLFKA

C. jeikeium, C. jejuni

PF-361

S. epidermidis, E. coli, E. faecalis,
NQVTEGIRLLVE
598

C. jejuni

PF-362

S. epidermidis, E. coli, P. aeruginosa,
NIERILKEKVWMIRCVE
599

C. albicans, E. faecalis,

C. jejuni

PF-363

B. subtilis, E. coli, P. aeruginosa,
SMLSVTVMCLMHASVAA
600

C. albicans, S. pneumoniae, E. faecalis
NQAMEKKV

PF-364

S. epidermidis, B. fragilis, P. aeruginosa,
LVNGIKI
601

C. jeikeium, C. jejuni

PF-365

S. epidermidis, B. subtilis, B. fragilis,
LYKQKIQLEEELEKLKDD
602

P. aeruginosa, C. albicans
RQ

PF-366

S. epidermidis, M. luteus, B. fragilis,
ALCSVIKAIELGIINVHLQ
603

P. mirabilis, E. coli, P. aeruginosa,

C. albicans, MRSA,

S. pneumoniae, E. faecalis, C. jeikeium,

C. jejuni

PF-367

B. subtilis

TKTPGTFTPGTGIQKTAVPL
604

PF-368

C. jeikeium, C. jejuni
MLKQTA
605

PF-369

B. subtilis, B. fragilis, E. coli, P. aeruginosa,
MSEAVNLLRGARYSQRY
606

C. albicans, S. pneumoniae,
AKNQVPYEVIIEK

C. jeikeium, C. jejuni

PF-370

S. epidermidis, E. coli, P. aeruginosa,
VIFLHKESGNLKEIFY
607

E. faecalis, C. jejuni

PF-371

S. epidermidis, B. fragilis, C. jejuni
TFIYNEF
608

PF-372

C. jeikeium, C. jejuni
KKQDKRIEDKYKRMKKGD
609

PF-373

S. epidermidis, E. coli, P. aeruginosa,
HFYLLFER
610

C. albicans, MRSA,

E. faecalis, C. jejuni

PF-374

S. epidermidis, B. subtilis, B. fragilis,
HLFFVKGMFILCQKNQIN
611

E. coli, P. aeruginosa, C. albicans,
DE

MRSA, S. pneumoniae,

E. faecalis, C. jeikeium, C. jejuni

PF-375

S. epidermidis, B. subtilis, B. fragilis,
MDSAKAQTMRTDWLAVS
612

E. coli, P. aeruginosa, C. albicans,
CLVASAYLRSMLA

S. pneumoniae, E. faecalis,

C. jeikeium, C. jejuni

PF-376

S. epidermidis, B. subtilis, B. fragilis,
MTVFEALMLAIAFATLIV
613

E. coli, P. aeruginosa, C. albicans,
KISNKNDKK

MRSA, S. pneumoniae,

E. faecalis, C. jeikeium, C. jejuni

PF-378

B. subtilis, B. fragilis, E. coli, P. aeruginosa,
ESAKSNLNFLMQEEWALF
614

C. jeikeium

LLL

PF-379

S. epidermidis, B. subtilis, B. fragilis,
VFVVLFIIYLASKLLTKLFP
615

E. coli, P. aeruginosa, C. albicans,
IKK

MRSA, S. pneumoniae,

E. faecalis, C. jeikeium, C. jejuni

PF-380

S. epidermidis, B. subtilis, B. fragilis,
KKIIPLITLFVVTLVG
616

E. coli, P. aeruginosa, C. albicans,

MRSA, S. pneumoniae,

E. faecalis, C. jeikeium, C. jejuni

PF-381

E. coli, P. aeruginosa, C. jejuni
QGANPCQQVGFTVNDPD
617

CRLAKTV

PF-382

S. epidermidis, B. subtilis, B. fragilis,
KYKCSWCKRVYTLRKDH
618

E. coli, P. aeruginosa, E. faecalis,
KTAR

C. jeikeium, C. jejuni

PF-383

S. epidermidis, B. subtilis, B. fragilis,
WSEIEINTKQSN
619

E. coli, C. jejuni

PF-384

E. faecalis, C. jeikeium, C. jejuni
HISKERFEAY
620

PF-385

S. epidermidis, E. coli, P. aeruginosa,
MIKKSILKIKYYVPVLISLT
621

C. albicans, E. faecalis
LILSA

PF-386

S. epidermidis, B. subtilis, B. fragilis,
FTLTLITTIVAILNYKDKKK
622

E. coli, P. aeruginosa, C. albicans,

S. pneumoniae, E. faecalis,

C. jeikeium, C. jejuni

PF-387

B. subtilis, E. coli, P. aeruginosa,
GAVGIAFFAGNMKQDKRI
623

E. faecalis, C. jeikeium, C. jejuni
ADRQNKKSEKK

PF-388

E. faecalis, C. jeikeium, C. jejuni
ITPLLDEIGKVCIDKISK
624

PF-389

S. epidermidis, C. albicans,
GLQFKEIAEEFHITTTALQ
625

MRSA, S. pneumoniae, E. faecalis,
QWHKDNGYPIYNKNNRK

C. jeikeium

PF-390

S. epidermidis, P. aeruginosa, C. albicans,
VVAYVITQVGAIRF
626

MRSA

PF-392

S. epidermidis, B. subtilis, S. pneumoniae,
DPAGCNDIVRKYCK
627

C. jeikeium, C. jejuni

PF-393

S. epidermidis, E. coli, C. albicans,
DLVQSILSEFKKSG
628

MRSA, S. pneumoniae,

C. jejuni

PF-394

S. epidermidis, MRSA, C. jejuni
VLKEECYQKN
629

PF-395

S. epidermidis, E. coli, P. aeruginosa,
YCVPLGNMGNMNNKIW
630

S. pneumoniae, E. faecalis,

C. jeikeium, C. jejuni

PF-396

S. epidermidis, E. coli, P. aeruginosa,
LIYTILASLGVLTVLQAILG
631

C. albicans, E. faecalis,
REPKAVKA

C. jeikeium

PF-397

S. epidermidis, MRSA, S. pneumoniae,
VEDLMEDLNA
632

E. faecalis, C. jejuni

PF-398

S. epidermidis, B. subtilis, B. fragilis,
ILVVLAGILLVVLSYVGIS
633

E. coli, P. aeruginosa, C. albicans,
KFKMNC

MRSA, S. pneumoniae,

E. faecalis, C. jeikeium, C. jejuni

PF-399

S. epidermidis, M. luteus, P. mirabilis,
FPIISALLGAIICIAIYSFIVN
634

E. coli, P. aeruginosa,
RKA

C. albicans, MRSA, S. pneumoniae,

E. faecalis, C. jejuni

PF-400

S. epidermidis, E. coli, S. pneumoniae,
VIAWKFRNKFENSGV
635

E. faecalis, C. jeikeium

PF-401

S. epidermidis, E. coli, P. aeruginosa,
YWLSRVTTGHSFAFEKPV
636

MRSA, E. faecalis, C. jejuni
PLSLTIK

PF-402

S. epidermidis, P. aeruginosa, E. faecalis,
FIDVLKSKINEFLN
637

C. jejuni

PF-403

E. coli, P. aeruginosa, S. pneumoniae,
LLSTEQLLKYYDGETFDG
638

E. faecalis, C. jeikeium,
FQLPSNE

C. jejuni

PF-404

S. epidermidis, E. coli, P. aeruginosa,
VLYFQATVV
639

E. faecalis, C. jeikeium,

C. jejuni

PF-405

S. epidermidis, E. coli, E. faecalis
LVRIEVDDLEEWYERNFI
640

PF-406

E. coli, C. jejuni
YLEMNADYLSNMDIFDEL
641

WEKYLENNK

PF-407

S. epidermidis, B. subtilis, E. coli,
KPKNKKEKTVISYEKLLS
642

P. aeruginosa, MRSA, S. pneumoniae,
MY

E. faecalis, C. jeikeium,

C. jejuni

PF-408

S. epidermidis, E. coli, P. aeruginosa,
YCVPLGNMGNMNNKIW
643

MRSA, E. faecalis, C. jeikeium,

C. jejuni

PF-409

S. epidermidis, MRSA, C. jeikeium,
DLVQSILSEFKKSG
644

C. jejuni

PF-410

S. epidermidis, M. luteus, B. fragilis,
FALELIALCRNLFIVYFP
645

P. mirabilis, E. coli, P. aeruginosa,

C. albicans, MRSA,

S. pneumoniae, E. faecalis

PF-411

M. luteus, B. subtilis, B. fragilis,
WVAVAILLNIALQTQLT
646

P. mirabilis, P. aeruginosa, C. albicans,

MRSA, S. pneumoniae,

E. faecalis, C. jeikeium, C. jejuni

PF-412

M. luteus, E. coli, C. albicans, C. jeikeium,
TSGWLGQLEQ
647

C. jejuni

PF-413

P. aeruginosa, C. albicans, C. jejuni
TFAGSIKIGVPDLVHVTFN
648

CKR

PF-414

E. coli, C. albicans, C. jeikeium
LLNKKLE
649

PF-416

S. pneumoniae, C. jeikeium
SKAGLYGKIERSDKRE
650

PF-417

S. epidermidis, C. jeikeium, C. jejuni
DSYFRS
651

PF-418

S. epidermidis, M. luteus, P. mirabilis,
FFLVHFYIRKRKGKVSIFL
652

E. coli, P. aeruginosa,
NYF

C. albicans, MRSA, S. pneumoniae,

E. faecalis, C. jeikeium,

C. jejuni

PF-421

C. jeikeium

KHCFEITDKTDVV
653

PF-422

C. albicans, MRSA, C. jeikeium
MSRKKYENDEKSQKKLKI
654

GRKSDVFYGIID

PF-423

S. epidermidis, M. luteus, E. coli,
AGKKERLLSFREQFLNKN
655

S. pneumoniae, E. faecalis, C. jeikeium
KKK

PF-424

S. epidermidis, C. albicans,
IAAFVTSRAFSDTVSPI
656

MRSA

PF-425

S. epidermidis, M. luteus, E. coli,
MMELVLKTIIGPIVVGVVL
657

P. aeruginosa, C. albicans,
RIVDKWLNKDK

MRSA, S. pneumoniae, C. jeikeium

PF-426

S. epidermidis, E. coli, P. aeruginosa,
MLQKYTQMISVTKCIITKN
658

C. albicans, MRSA,
KKTQENVDAYN

S. pneumoniae, E. faecalis, C. jeikeium

PF-427

M. luteus, P. aeruginosa, C. albicans,
YVLEYHGLRATQDVDAF
659

C. jejuni

MAL

PF-428

S. epidermidis, C. albicans, E. faecalis,
ENEESIF
660

C. jeikeium

PF-429

S. epidermidis, S. pneumoniae, C. jeikeium
AATLICVGSGIMSSL
661

PF-430

S. epidermidis, M. luteus, E. coli,
AVVCGYLAYTATS
662

S. pneumoniae, E. faecalis, C. jeikeium,

C. jejuni

PF-431

S. epidermidis, M. luteus, E. coli,
VAYAAICWW
663

P. aeruginosa, MRSA, S. pneumoniae,

E. faecalis, C. jeikeium,

C. jejuni

PF-432

S. epidermidis, M. luteus, E. coli,
FNGDSEFFLCIAF
664

P. aeruginosa, C. albicans,

MRSA, S. pneumoniae, E. faecalis,

C. jeikeium, C. jejuni

PF-433

S. epidermidis, E. coli, S. pneumoniae,
MRKEFHNVLSSGQLLADK
665

C. jeikeium

RPARDYNRK

PF-434

S. epidermidis, M. luteus, S. pneumoniae,
GQLLADKRPARDYNRK
666

C. jeikeium

PF-435

C. jeikeium

MSRWDGHSDKGEAPAGK
667

PPMHGFGLNGENK

PF-436

C. jeikeium

KKHVLVGKQEKNG
668

PF-438

S. epidermidis, E. coli, S. pneumoniae,
QPYFQNQFKKITGYTPLQ
669

C. jeikeium, C. jejuni
YRKEKR

PF-439

S. epidermidis, M. luteus, B. fragilis,
RVLVLKKFHGIMDGNRN
670

P. mirabilis, E. coli, P. aeruginosa,
VAVFFVGQ

C. albicans, MRSA,

S. pneumoniae, E. faecalis, C. jeikeium,

C. jejuni

PF-440

S. epidermidis, M. luteus, P. mirabilis,
MFIISPDLFNIAVILYILFFI
671

E. coli, P. aeruginosa,
HDILLLILS

C. albicans, MRSA, S. pneumoniae,

E. faecalis, C. jeikeium,

C. jejuni

PF-441

C. jeikeium

TQVHKMARGIDPGPANGI
672

YR

PF-442

S. epidermidis, E. coli, C. albicans,
MQIFYIKTKIFLSFFLFLLIF
673

S. pneumoniae, E. faecalis
SQCFYKIEE

PF-443

S. epidermidis, M. luteus, P. mirabilis,
KLLYFFNYFENLQQVHLL
674

E. coli, P. aeruginosa,
VQL

C. albicans, S. pneumoniae, E. faecalis,

C. jeikeium, C. jejuni

PF-444

M. luteus, C. albicans, S. pneumoniae,
MAAKLWEEGKMVYASSA
675

C. jeikeium

SMTKRLKLAMSKV

PF-445

M. luteus, S. pneumoniae, C. jeikeium
ASMTKRLKLAMSKV
676

PF-446

M. luteus, C. jeikeium
SGNEKV
677

PF-447

S. epidermidis, M. luteus, E. coli,
IDKSRNKDQFSHIFGLYNI
678

S. pneumoniae

CSG

PF-448

S. epidermidis, M. luteus, P. mirabilis,
SLQSQLGPCLHDQRH
679

E. coli, S. pneumoniae,

E. faecalis, C. jeikeium, C. jejuni

PF-450

S. epidermidis, M. luteus, E. coli,
HRNLIILQRTIFI
680

P. aeruginosa, C. albicans,

MRSA, S. pneumoniae, E. faecalis,

C. jeikeium, C. jejuni

PF-451

S. epidermidis, M. luteus, E. coli,
MVNYIIGSYMLYREQNNN
681

P. aeruginosa, C. albicans,
EALRKFDITLAM

MRSA, S. pneumoniae, E. faecalis,

C. jeikeium, C. jejuni, M. smegmatis

PF-452

M. luteus, P. aeruginosa, C. albicans,
MNNWIKVAQISVTVINEVI
682

S. pneumoniae, E. faecalis,
DIMKEKQNGGK

C. jeikeium, M. smegmatis

PF-453

M. luteus, E. coli, P. aeruginosa,
IIQDIAHAFGY
683

S. pneumoniae, E. faecalis, C. jeikeium,

C. jejuni

PF-454

S. epidermidis, M. luteus, E. coli,
MSVFVPVTNIFMFIMSPIF
684

P. aeruginosa, C. albicans,
NVNLLHFKVYI

MRSA, S. pneumoniae, E. faecalis,

C. jeikeium, C. jejuni, M. smegmatis

PF-456

C. albicans, MRSA, E. faecalis,
TCVKPRTIN
685

C. jeikeium, C. jejuni

PF-457

C. albicans, S. pneumoniae, E. faecalis,
INKYHHIA
686

C. jeikeium

PF-458

S. epidermidis, M. luteus, E. coli,
ISLIIFIMLFVVALFKCITNY
687

P. aeruginosa, C. albicans,
KHQS

MRSA, S. pneumoniae, E. faecalis,

C. jeikeium, C. jejuni

PF-459

P. aeruginosa

EKRMSFNENQSHRPLL
688

PF-460

S. epidermidis, M. luteus, P. mirabilis,
MEHVLPFQNTPPNIVIIYK
689

E. coli, P. aeruginosa,
DFTHLKSITFS

C. albicans, MRSA, S. pneumoniae,

E. faecalis, C. jeikeium,

C. jejuni, M. smegmatis

PF-461

E. coli, S. pneumoniae
MTLAIKNCSVTKCLGFGD
690

FVNDDSDSYFDA

PF-462

E. faecalis, C. jeikeium
KNKTDTL
691

PF-463

S. epidermidis, E. coli, P. aeruginosa,
MVILVFSLIFIFTDNYLVY
692

C. albicans, S. pneumoniae,
QSKSIKEDVMI

E. faecalis, M. smegmatis

PF-464

S. epidermidis, C. albicans,
VDMVNRFLGN
693

MRSA, S. pneumoniae, E. faecalis,

C. jeikeium, C. jejuni

PF-465

S. epidermidis, M. luteus, E. coli,
KPVGKALEEIADGKIEPVV
694

P. aeruginosa, C. albicans,
PKEYLG

MRSA, S. pneumoniae, E. faecalis,

C. jeikeium, C. jejuni

PF-466
MRSA, C. jeikeium, C. jejuni
VRKSDQ
695

PF-467
MRSA, E. faecalis, C. jeikeium,
YYKDYFKEI
696

C. jejuni

PF-469

S. epidermidis, M. luteus, P. mirabilis,
YKVNYNNIDNHFNTLRH
697

E. coli, C. albicans,

MRSA, S. pneumoniae, E. faecalis,

C. jeikeium, C. jejuni

PF-470

M. luteus, E. coli, MRSA, E. faecalis,
PYSDSYATRPHWEQHRAR
698

C. jeikeium, C. jejuni

PF-471

S. epidermidis, M. luteus, E. coli,
MVGKIRGVTPRNDLLNAN
699

P. aeruginosa, C. albicans,
ITGQLNLNYRLI

MRSA, S. pneumoniae, E. faecalis,

C. jeikeium, C. jejuni

PF-472

S. epidermidis, E. coli, P. aeruginosa,
MHISHLLDEVEQTEREKA
700

C. albicans, MRSA,
VNVLENMNGNVI

S. pneumoniae, E. faecalis, C. jeikeium

PF-473

S. epidermidis, E. coli, P. aeruginosa,
MAADIISTIGDLVKWIIDT
701

C. albicans, MRSA,
VNKFKK

S. pneumoniae, E. faecalis

PF-474

S. epidermidis, M. luteus, P. mirabilis,
MHRNLVLVKMEPIPHIMII
702

E. coli, P. aeruginosa,
ANQIGIIIEKA

C. albicans, MRSA, S. pneumoniae,

E. faecalis, C. jeikeium,

C. jejuni, M. smegmatis

PF-475

S. epidermidis, M. luteus, P. mirabilis,
MREKVRFTQAFKLFWTN
703

E. coli, P. aeruginosa,
YFNFKGRSRRSEY

C. albicans, MRSA, S. pneumoniae,

E. faecalis, C. jeikeium,

C. jejuni

PF-476

M. luteus, P. mirabilis, C. albicans,
WADAQYKLCENCSE
704

S. pneumoniae, E. faecalis,

C. jeikeium, C. jejuni

PF-477

S. epidermidis, M. luteus, C. albicans,
HKNKLNIPHIKS
705

S. pneumoniae, C. jeikeium,

C. jejuni

PF-478

S. epidermidis, M. luteus, P. mirabilis,
HLFILKSHLKPFPPFRYTYD
706

E. coli, C. albicans, S. pneumoniae,

E. faecalis, C. jeikeium,

C. jejuni

PF-479

S. epidermidis, M. luteus, P. mirabilis,
AYILKRREEKNK
707

E. coli, C. albicans, S. pneumoniae,

E. faecalis, C. jeikeium,

C. jejuni

PF-480

S. epidermidis, M. luteus, P. mirabilis,
MVEILVNTAISVYIVALYT
708

E. coli, P. aeruginosa,
QWLSTRDNLKA

C. albicans, MRSA, S. pneumoniae,

E. faecalis, C. jeikeium,

C. jejuni, M. smegmatis

PF-481

C. jeikeium

DELYEIMDKVIEEFNKDIE
709

QNNNNGNNEDLTENKIN

PF-482

S. epidermidis, M. luteus, P. mirabilis,
LVGYVRTSGTVRSYKIN
710

E. coli, P. aeruginosa,

C. albicans, MRSA, S. pneumoniae,

E. faecalis, C. jeikeium,

C. jejuni

PF-483

P. mirabilis, C. jeikeium, C. jejuni
EDNKDKKDKKDK
711

PF-484

S. epidermidis, M. luteus, P. mirabilis,
HKKDIRKQVFKN
712

E. coli, E. faecalis, C. jeikeium,

C. jejuni

PF-486

S. mutans

MQKEGEEDY
713

PF-487

S. mutans, E. coli
MYKAIAVLAMTIMAFFIF
714

VYPFFIVGLILG

PF-488

S. mutans, E. coli
YPNEQGHHKNNLKNIIIE
715

PF-489

S. mutans

KVDRVSTTITEKIK
716

PF-490

S. mutans, E. coli
RLILVSGNATVQK
717

PF-491

S. mutans, E. coli
IHQYSSKPDIVGQEAKTVQ
718

QINS

PF-492

S. mutans, B. subtilis, E. coli
IQIDAASFYSISKSTIK
719

PF-493

S. mutans, B. subtilis, E. coli
PGAFFFCRGRGCWCGIGW
720

PF-494

S. mutans

FTEPLRPLQAKGQIISIKPS
721

TSSS

PF-495

S. mutans, E. coli
KGIYKKRTY
722

PF-496

S. mutans, E. coli
EVTKRLVALAQQQLRG
723

PF-497

S. mutans, B. subtilis, E. coli
LVLRICTDLFTFIKWTIKQ
724

RKS

PF-498

S. mutans, E. coli
MSEEEEVSEKVYNYLRRN
725

EFFEVRKEEFSA

PF-499

S. mutans, E. coli
VYSFLYVLVIVRKLLSMK
726

KRIERL

PF-500

S. mutans, E. coli
MGIFKEEKIKFIDCKGEEVI
727

LKIKIKDIKK

PF-501

S. mutans

GSTAHKSPIGSTNNQWGM
728

KKTPTD

PF-502

S. mutans

NKGKQMQDQTGKQPIVD
729

NG

PF-503

S. mutans

VVTLKDIVAVIEDQGYDVQ
730

PF-504

S. mutans, E. coli
ILSVELSTKTSASGS
731

PF-505

S. mutans

GYTKDPGTGI
732

PF-506

S. mutans, E. coli
SGRGFALIVVLFILLIIVGA
733

ACIR

PF-507

S. mutans, E. coli
LALSIANLFKKKA
734

PF-508

S. mutans

VSTFGKVVKVVDEK
735

PF-509

S. mutans, B. subtilis, E. coli
EAKVQAKGEQIACNNY
736

PF-510

S. mutans, E. coli
WYLYKKQSNQNDRGIPK
737

PF-511

E. coli, P. aeruginosa, S. pneumoniae,
VMQSLYVKPPLILVTKLA
738

C. jeikeium

QQN

PF-512

S. pneumoniae, C. jeikeium
SFMPEIQKNTIPTQMK
739

PF-513

C. albicans

SNGVGLGVGIGSGIRF-
740

NH2

PF-514

S. epidermidis, E. coli, P. aeruginosa,
QRFYKLFYHIDLTNEQAL
741

C. albicans, MRSA,
KLFQVK

S. pneumoniae, E. faecalis, C. jeikeium

PF-515

S. epidermidis, C. albicans, S. pneumoniae,
DKSTQDKDIKQAKLLAQE
742

C. jeikeium

LGL-NH2

PF-517

C. jejuni

VKPTMTASLISTVC
743

PF-518

S. epidermidis, E. coli, P. aeruginosa,
SFYSKYSRYIDNLAGAIFL
744

C. albicans, MRSA,
FF

S. pneumoniae, E. faecalis

PF-519

M. luteus, E. faecalis, C. jeikeium
YLVYSGVLATAAAF-NH2
745

PF-520

S. epidermidis, M. luteus, E. coli,
LGLTAGVAYAAQPTNQPT
746

C. albicans, MRSA, S. pneumoniae,
NQPTNQPTNQPTNQPTNQ

E. faecalis, C. jeikeium,
PRW-NH2

C. jejuni

PF-521

S. epidermidis, E. coli, P. aeruginosa,
CGKLLEQKNFFLKTR
747

S. pneumoniae, E. faecalis

PF-522

S. epidermidis, E. coli, P. aeruginosa,
FELVDWLETNLGKILKSK
748

S. pneumoniae, E. faecalis
SA-NH2

PF-523

S. epidermidis, M. luteus, C. albicans,
ASKQASKQASKQASKQAS
749

S. pneumoniae, C. jeikeium,
KQASRSLKNHLL

C. jejuni

PF-524

S. epidermidis, E. coli, P. aeruginosa,
PDAPRTCYHKPILAALSRI
750

C. albicans, MRSA,
VVTDR

S. pneumoniae, E. faecalis, C. jeikeium

PF-526

S. epidermidis, E. coli, P. aeruginosa,
VLLLFIFQPFQKQLL-NH2
751

C. albicans, MRSA,

S. pneumoniae, E. faecalis, C. jeikeium,

C. jejuni

PF-527

S. epidermidis, M. luteus, P. mirabilis,
GSVIKKRRKRMAKKKHR
752

E. coli, P. aeruginosa,
KLLKKTRIQRRRAGK

MRSA, S. pneumoniae, E. faecalis,

C. jeikeium, C. jejuni

PF-528

S. epidermidis, P. aeruginosa, C. albicans,
LVDVVVLIRRHLPKSCS-
753

MRSA, S. pneumoniae,
NH2

E. faecalis

PF-529

S. epidermidis, E. coli, C. albicans,
LSEMERRRLRKRA-NH2
754

MRSA, S. pneumoniae,

E. faecalis, C. jeikeium

PF-537

S. epidermidis, M. luteus, P. mirabilis,
LANDYYKKTKKSW
755

E. coli, C. albicans,

MRSA, S. pneumoniae, E. faecalis,

C. jeikeium

PF-539

S. epidermidis, M. luteus, B. subtilis,
SIILTKKKRRKIPLSIDSQIY
756

P. mirabilis, E. coli, P. aeruginosa,
KYTFKQ

C. albicans, MRSA,

S. pneumoniae, E. faecalis, C. jeikeium

PF-540

C. albicans

KSILILIKVIFIGQTTIIL
757

PF-542

C. jeikeium

KKDNPSLNDQDKNAVLN
758

LLALAK

PF-543

S. epidermidis, M. luteus, B. subtilis,
NILFGIIGFVVAMTAAVIV
759

P. mirabilis, E. coli, P. aeruginosa,
TAISIAK

C. albicans, MRSA,

S. pneumoniae, E. faecalis, C. jeikeium

PF-544

S. epidermidis, M. luteus, P. mirabilis,
FGEKQMRSWWKVHWFHP
760

P. aeruginosa, MRSA,

S. pneumoniae, E. faecalis, C. jeikeium,

C. jejuni

PF-545

S. epidermidis, E. coli, P. aeruginosa,
RESKLIAMADMIRRRI-
761

S. pneumoniae, E. faecalis,
NH2

C. jeikeium

PF-546

S. epidermidis, E. coli, C. albicans,
PIIAPTIKTQIQ
762

S. pneumoniae, E. faecalis,

C. jeikeium

PF-547

S. epidermidis, E. coli, P. aeruginosa,
WSRVPGHSDTGWKVWHR
763

C. albicans, MRSA,
W-NH2

S. pneumoniae, E. faecalis

PF-548

M. luteus, P. mirabilis, E. coli, P. aeruginosa,
ARPIADLIHFNSTTVTASG
764

C. albicans, S. pneumoniae,
DVYYGPG

E. faecalis, C. jeikeium,

C. jejuni

PF-549

E. coli, C. albicans, S. pneumoniae,
TGIGPIARPIEHGLDS
765

C. jeikeium

PF-550

S. pneumoniae

STENGWQEFESYADVGV
766

DPRRYVPL

PF-551

S. pneumoniae

QVKEKRREIELQFRDAEK
767

KLEASVQAE

PF-552

S. pneumoniae

ELDKADAALGPAKNLAPL
768

DVINRS

PF-553

S. epidermidis, E. coli, P. aeruginosa,
LTIVGNALQQKNQKLLLN
769

C. albicans, MRSA,
QKKITSLG

S. pneumoniae, C. jeikeium

PF-554

S. pneumoniae

AKNFLTRTAEEIGEQAVR
770

EGNINGP

PF-555
MRSA, S. pneumoniae, C. jeikeium
EAYMRFLDREMEGLTAA
771

YNVKLFTEAIS

PF-556

S. epidermidis, M. luteus, B. fragilis,
SLQIRMNTLTAAKASIEAA
772

P. mirabilis, E. coli, P. aeruginosa,

C. albicans, MRSA,

S. pneumoniae, E. faecalis

PF-557

S. pneumoniae

AANKAREQAAAEAKRKA
773

EEQAR

PF-558

S. epidermidis, E. coli, C. albicans,
ADAPPPLIVRYS
774

C. jeikeium, C. jejuni

PF-559

S. epidermidis, M. luteus, C. albicans,
SRPGKPGGVSIDVSRDRQ
775

C. jeikeium, C. jejuni
DILSNYP

PF-560

S. epidermidis, M. luteus, E. coli,
FGNPFRGFTLAMEADFKK
776

S. pneumoniae, C. jeikeium, C. jejuni
RK

PF-562

S. epidermidis, M. luteus, P. mirabilis,
TPEQWLERSTVVVTGLLN
777

E. coli, P. aeruginosa,
RK

C. albicans, MRSA, S. pneumoniae,

E. faecalis, C. jeikeium,

C. jejuni

PF-563

S. epidermidis, M. luteus, C. jeikeium
RPELDNELDVVQNSASLD
778

KLQASYN

PF-564

S. epidermidis, C. albicans, S. pneumoniae,
TIILNDQINSLQERLNKLN
779

C. jeikeium

AETDRR

PF-566

P. mirabilis, S. pneumoniae
EAQQVTQQLGADFNAITT
780

PTATKV

PF-567

S. epidermidis, P. aeruginosa, C. albicans,
QQRVKAVDASLSQVSTQV
781

MRSA, S. pneumoniae,
SGAVASA

C. jeikeium

PF-568

S. epidermidis

TQAVQVKTAQAQQQ
782

PF-569

M. luteus, P. mirabilis, S. pneumoniae,
KSKISEYTEKEFLEFVEDIY
783

E. faecalis, C. jeikeium
TNNK

PF-570

S. pneumoniae, C. jeikeium
KKFPTEESHIQAVLEFKKL
784

TEHPSG

PF-572

S. epidermidis, M. luteus, E. coli,
WRASKGLPGFKAG
785

S. pneumoniae, C. jeikeium

PF-573

S. epidermidis, S. pneumoniae
EKKLIVKLIDSIGKSHEEIV
786

GAG

PF-575

M. luteus, E. coli, C. albicans,
LNFRAENKILEKIHISLIDT
787

MRSA, S. pneumoniae, E. faecalis,
VEGSA

C. jeikeium

PF-576

M. luteus, P. mirabilis, E. coli, P. aeruginosa,
AYSGELPEPLVRKMSKEQ
788

C. albicans, S. pneumoniae
VRSVMGK

PF-577

S. epidermidis, M. luteus, P. mirabilis,
PFETRESFRVPVIGILGGW
789

E. coli, P. aeruginosa,
DYFMHP

C. albicans, MRSA, S. pneumoniae,

E. faecalis

PF-578

S. epidermidis, M. luteus, P. mirabilis,
QKANLRIGFTYTSDSNVC
790

P. aeruginosa, C. albicans,
NLTFALLGSK

MRSA, S. pneumoniae,

E. faecalis, C. jeikeium

PF-579

S. epidermidis, M. luteus, P. mirabilis,
MILVCAAVIWGRVLFILKF
791

E. coli, P. aeruginosa,
PIYFSIRLAFL

C. albicans, MRSA, S. pneumoniae,

E. faecalis, C. jeikeium,

C. jejuni

PF-580

S. epidermidis, M. luteus, E. coli,
EILNNNQVIKELTMKYKT
792

P. aeruginosa, C. albicans,
QFESNLGGWTARARR

MRSA, S. pneumoniae, E. faecalis,

C. jeikeium

PF-581

S. epidermidis, M. luteus, E. coli,
WTARARR
793

P. aeruginosa, C. albicans,

MRSA, S. pneumoniae, E. faecalis,

C. jeikeium

PF-583

S. epidermidis, M. luteus, E. coli,
KFQGEFTNIGQSYIVSASH
794

P. aeruginosa, C. albicans,
MSTSLNTGK

MRSA, S. pneumoniae, E. faecalis,

C. jeikeium

PF-584

S. epidermidis, M. luteus, P. mirabilis,
SYIKNLSNQKFLIAF
795

C. albicans, MRSA, S. pneumoniae,

E. faecalis, C. jeikeium

PF-585

S. epidermidis, E. coli, C. albicans,
DYNHLLNVVQDWVNTN
796

MRSA, S. pneumoniae,

C. jeikeium

PF-586

S. epidermidis, M. luteus, E. coli,
FFNQANYFFKEF
797

P. aeruginosa, MRSA, S. pneumoniae,

E. faecalis, C. jeikeium,

C. jejuni

PF-587

S. epidermidis, M. luteus, E. coli,
ASGKYQSYLLNVYVDSK
798

MRSA, S. pneumoniae, E. faecalis,
KDRLDIFDKLKAKAKFVL

C. jeikeium

PF-588

S. epidermidis, E. coli, C. albicans,
ESVEAIKAKAIK
799

E. faecalis, C. jeikeium,

C. jejuni

PF-589

S. epidermidis, C. albicans,
APLRIDEIRNSNVIDEVLD
800

MRSA, S. pneumoniae
CAPKKQEHFFVVPKIIE

PF-590

S. epidermidis, M. luteus, E. coli,
YYQAKLFPLL
801

E. faecalis, C. jeikeium

PF-592

S. epidermidis, M. luteus, P. mirabilis,
IMKNYKYFKLFIVKYALF
802

E. coli, P. aeruginosa,

C. albicans, MRSA, S. pneumoniae,

E. faecalis, C. jeikeium,

C. jejuni

PF-593

C. jeikeium

MEISTLKKEKLHVKDELS
803

QYLANYKK

PF-594

C. jeikeium

IVSAIV
804

PF-595

S. epidermidis, M. luteus, P. mirabilis,
LQNKIYELLYIKERSKLCS
805

E. coli, P. aeruginosa,

C. albicans, MRSA, S. pneumoniae,

E. faecalis, C. jeikeium,

C. jejuni

PF-596

S. epidermidis, M. luteus, P. mirabilis,
SKMWDKILTILILILELIRE
806

E. coli, P. aeruginosa,
LIKL

MRSA, E. faecalis, C. jeikeium,

C. jejuni

PF-597

P. mirabilis

DEIKVSDEEIEKFIKENNL
807

PF-598

S. epidermidis, M. luteus, P. mirabilis,
MKFMLEVRNKAISAYKEI
808

P. aeruginosa, C. albicans,
TRTQI

MRSA, S. pneumoniae,

C. jeikeium

PF-599

S. epidermidis, P. mirabilis, E. coli,
LFEIFKPKH
809

C. albicans, MRSA, S. pneumoniae,

C. jeikeium

PF-600

S. epidermidis, M. luteus, B. subtilis,
TKKIELKRFVDAFVKKSY
810

P. mirabilis, E. coli, P. aeruginosa,
ENYILERELKKLIKAINEEL

MRSA, S. pneumoniae,
PTK

E. faecalis, C. jeikeium

PF-601

C. jeikeium

YRVTVKALE
811

PF-602

P. mirabilis, C. jeikeium
LEKEKKEYIEKLFKTK
812

PF-603

S. epidermidis, M. luteus, B. subtilis,
IDKLKKMNLQKLSYEVRI
813

E. coli, P. aeruginosa,
SQDGKSIYARIK

MRSA, S. pneumoniae, E. faecalis,

C. jeikeium

PF-604

S. epidermidis, C. albicans, C. jeikeium
LMEQVEV
814

PF-605

S. epidermidis, M. luteus, P. mirabilis,
HYRWNTQWWKY
815

E. coli, P. aeruginosa,

C. albicans, MRSA, S. pneumoniae,

E. faecalis, C. jeikeium,

C. jejuni

PF-607

S. epidermidis, P. mirabilis, E. coli,
YIESDPRKFDYIFGAIRDH
816

MRSA, S. pneumoniae, C. jeikeium

PF-609

P. mirabilis, E. coli, S. pneumoniae
TEIKLDNNEYLVLNLDDIL
817

GILK

PF-610

S. epidermidis, M. luteus, P. mirabilis,
VFLKLKTSKIDLASIIFYP
818

E. coli, P. aeruginosa,

C. albicans, MRSA, S. pneumoniae,

E. faecalis, C. jeikeium,

C. jejuni

PF-612

S. epidermidis, M. luteus, P. mirabilis,
GTTLKYGLERQLKIDIHPE
819

E. coli, P. aeruginosa,
ITIINLNGGADEFAKL

C. albicans, MRSA, S. pneumoniae,

E. faecalis, C. jeikeium

PF-613

C. jeikeium

ADEFAKL
820

PF-614

S. epidermidis, E. coli, C. jeikeium
GLDIYA
821

PF-615

S. epidermidis, M. luteus, P. mirabilis,
FLNRFIFYIFTVKTKSALIK
822

E. coli, P. aeruginosa,
NLFLD

C. albicans, MRSA, S. pneumoniae,

C. jeikeium, C. jejuni

PF-616

C. jeikeium

IVFVVTKEKK
823

PF-617

P. aeruginosa, C. albicans
PMNAAEPE
824

PF-619

S. epidermidis, M. luteus, B. subtilis,
WSRVPGHSDTGWKVWHRW
825

P. mirabilis, E. coli, P. aeruginosa,

C. albicans, MRSA,

S. pneumoniae, E. faecalis, C. jeikeium

PF-621

S. epidermidis, C. albicans
PPSSFLV
826

PF-622

S. epidermidis, P. aeruginosa, C. albicans,
TREDVFSVRLINNIVNKQA
827

S. pneumoniae, E. faecalis,

C. jeikeium

PF-623

S. epidermidis, P. aeruginosa,
VLFAVYLGALDWLFSWL
828

MRSA, S. pneumoniae, E. faecalis,
TQKM

C. jeikeium

PF-625

S. epidermidis, M. luteus, S. pneumoniae,
SDSTNNARTRKKARDVTT
829

C. jeikeium

KDIDK

PF-626

S. epidermidis, M. luteus, E. coli,
KYDFDDFEPEEA
830

C. albicans, MRSA, S. pneumoniae,

E. faecalis, C. jeikeium

PF-627

S. epidermidis, P. aeruginosa,
INDLLSYFTLHEK
831

MRSA, S. pneumoniae, E. faecalis,

C. jeikeium

PF-629

S. epidermidis, P. aeruginosa, C. albicans,
GLAAIATVFALY
832

MRSA, S. pneumoniae,

E. faecalis, C. jeikeium

PF-630

S. epidermidis, M. luteus, P. mirabilis,
IPATPIIHS
833

P. aeruginosa, C. albicans,

MRSA, S. pneumoniae,

E. faecalis, C. jeikeium

PF-631

S. epidermidis, P. aeruginosa, C. albicans,
LIIYFSKTGNTARATRQI
834

MRSA, S. pneumoniae,

E. faecalis, C. jeikeium

PF-632

S. epidermidis, P. aeruginosa, C. albicans,
TTIQGVASLEKHGFRYTII
835

MRSA, S. pneumoniae,
YPTRI

E. faecalis, C. jeikeium

PF-634

S. epidermidis, M. luteus, P. mirabilis,
MPKARPVNHNKKKSKITI
836

E. coli, P. aeruginosa,
KSNFTLFYMFNP

C. albicans, MRSA, S. pneumoniae,

E. faecalis, C. jeikeium

PF-635

S. epidermidis, P. aeruginosa, C. albicans,
MNAHGHSLIFQKMIVHAF
837

MRSA, S. pneumoniae,
AFFSKQKNYLYF

E. faecalis, C. jeikeium

PF-636

S. epidermidis, C. albicans,
LVRLA
838

MRSA, S. pneumoniae, E. faecalis

PF-637

S. epidermidis, P. aeruginosa, C. albicans,
SRIKQDARSVRKYDRIGIF
839

MRSA, S. pneumoniae,
FYSFKSA

E. faecalis, C. jeikeium

PF-638

S. epidermidis, C. albicans,
TFILPK
840

MRSA, C. jeikeium

PF-639

S. pneumoniae, C. jeikeium
QATQIKSWIDRLLVSED
841

PF-640

C. albicans

MGDINRNF
842

PF-641

S. epidermidis, M. luteus, E. coli,
SWKCHHLAIGGSWKCHH
843

C. albicans, MRSA, E. faecalis,
LAI

C. jeikeium, C. jejuni

PF-642

M. luteus, MRSA, C. jeikeium
FTTPMIGIPAGLLGGSYYL
844

KRREEKGK

PF-643

Mycobacteria spp
VRCRL
845

PF-644

Mycobacteria spp
TSGLIIGENGLNGL
846

PF-645

Mycobacteria spp
SNSVQQG
847

PF-646

Mycobacteria spp
APASPGRRPG
848

PF-647

Mycobacteria spp
GTFLGQKCAAATAS
849

PF-648

S. mutans, E. coli
ARRYPAAGS
850

PF-649

Mycobacteria spp
CPRYPFVDVGPAGPWRAR
851

WRVGS

PF-650

Mycobacteria spp
IRSDQPGRQSRSSPRWPTG
852

AGRHR

PF-651

Mycobacteria spp
PRWPTGAGRHR
853

PF-652

Mycobacteria spp
FLAPARPDLQAQRQALAQ
854

PF-653

Mycobacteria spp
QSVHPLPAETPVADVI
855

PF-654

Mycobacteria spp
LSGRLAGRR
856

PF-655

M. smegmatis

DAPCFDDQFGDLKCQMC
857

PF-656

Mycobacteria spp
RGMFVPFHDVDCVQ
858

PF-657

Mycobacteria spp
YVANYTITQFGRDFDDRL
859

AVAIHFA

PF-658

Mycobacteria spp
PTTPPPTTPPEIPTGGTVIST
860

PF-659

Mycobacteria spp
TVIST
861

PF-660

Mycobacteria spp
TDPQATAAPRRRTSPR
862

PF-661

Mycobacteria spp
PDEDIRRRAILPPAGPCRP
863

MSPE

PF-662

Mycobacteria spp
GKQSRAHGPVASRREFRR
864

KSG

PF-663

Mycobacteria spp
ATLIPRKA
865

PF-664

M. smegmatis

DQLCVEYPARVSTG
866

PF-665

Mycobacteria spp
VLRVATAVGEVPTGL
867

PF-666

Mycobacteria spp
PNRRSRPR
868

PF-667

Mycobacteria spp
PAHQRLRIDQRLVADRDM
869

VQDYES

PF-668

Mycobacteria spp
TNAESMALAFRGRVHMS
870

VNIAGLT

PF-669

Mycobacteria spp
RADRIESYPADGDRVITL
871

WRNPYR

PF670

Mycobacteria spp
TVIVAPMHSGV
872

PF-671

S. mutans, E. coli
TVSAFRTVH
873

PF-673

S. mutans, E. coli
VRRLRM
874

PF-674

S. mutans, E. coli
DGCDSEPALTYR
875

PF-675

Mycobacteria spp
EIIPISPTRRCEMHTMSSAE
876

YRGL

PF-676

S. mutans, E. coli
AEYRGL
877

PF-677

Mycobacteria spp
TCRGAGMH
878

PF-678

Mycobacteria spp
RDRRWTRRDMYDWLESA
879

RV

PF-679

S. mutans, E. coli
CRARFIRR
880

PF-680

Mycobacteria spp
ADPHPTTGI
881

PF-681

M. smegmatis

TALTTVGVSGARLITYCV
882

GVEDI

PF-682

Mycobacteria spp
RRGKSEQGLSRR
883

PF-683

Mycobacteria spp
LWPVA
884

PF-684

Mycobacteria spp
RKLSLASGFALWRRSLV
885

PF-685

Mycobacteria spp
PTLWLACL
886

PF-686

M. smegmatis

LAVLMGYIGYRGWSGKR
887

HINRQ

PF-687

Mycobacteria spp
AKRVLSLAVAPHRRQPVQ
888

GT

PF-688

Mycobacteria spp
ARNHAVIPAG
889

PF-689

S. mutans, E. coli
SAPSG
890

PF-690

Mycobacteria spp
MIPLAGDPVSSHRTVEFG
891

VLGTYLVSGGSL

PF-691

Mycobacteria spp
HRTVEFGVLGTYLVSGGSL
892

PF-692

Mycobacteria spp
GVAREDPLEPDPLAPIIDD
893

SR

PF-693

Mycobacteria spp
PDPAR
894

PF-694

Mycobacteria spp
DLIRPLYSMSAPSVA
895

PF-695

Mycobacteria spp
ALSVMLGNIPLVVPNANQL
896

PF-696

Mycobacteria spp
IRSGISAAYARPLR
897

PF-697

Mycobacteria spp
RADARAK
898

PF-698

Mycobacteria spp
SSGRAGVKCRRPTGR
899

PF-699

Mycobacteria spp
GRAGVKCRRPTGR
900

PF-700

Mycobacteria spp
LNWPFTGR
901

PF-701

S. mutans

PRGAQSGHG
902

PF-702

Mycobacteria spp
LSGRLAGRR
903

PF-703

Mycobacteria spp
MTTVDNIVGLVIAVALMA
904

FLFAALLFPEKF

PF-704

Mycobacteria spp
APAARAAL
905

PF-705

S. mutans, E. coli
GEEEGTVAD
906

PF-706

L. pneumophila

LGYGAWIGCGLGLNGFHR
907

ID

PF-707

S. mutans, E. coli
IDPESIVTTNNKQDNVDEQ
908

PF-709

S. mutans

NKKHSPMD
909

PF-711

S. mutans

KTAGPTGTIYKTN
910

PF-712

S. mutans, E. coli
QIYRHVHKVQAKSANLRLY
911

PF-714

L. pneumophila

FVVTQRMLRMYKK
912

PF-716

S. mutans

HGENHHHKSDEKDNDSSE
913

KKD

PF-717

E. coli

PQSEVTFENIYAPKANGG
914

GLYGI

PF-720

S. mutans

SLDMGK
915

PF-724

L. pneumophila

CYRFLTPKRPTRIS
916

PF-727

S. mutans, E. coli
AYARCRHDYPFTLGQMQ
917

TH

PF-728

S. mutans, E. coli
AIGQEQDRREYYYYSGYP
918

YYY

PF-731

L. pneumophila

RHKLIRLPLSESVFCFLNN
919

PKI

PF-732

E. coli

DRPSQTTHHTLSSSRITGPS
920

PF-733

S. mutans, E. coli
VISRQMGSEAVLELFIIM
921

PF-735

S. mutans, E. coli
YDPLFPNDKN
922

PF-737

S. epidermidis, S. pneumoniae
KSSGSSASASSTAGGSSSK
923

PF-738

S. epidermidis, C. albicans, C. jeikeium
KSGATSAASGAKSGASS
924

PF-741

S. epidermidis, M. luteus, P. mirabilis,
AKREDTVAAQIGANILNLIQ
925

P. aeruginosa, C. albicans,

MRSA, S. pneumoniae,

E. faecalis

PF-744

S. epidermidis, M. luteus, E. coli,
LGVGTFVGKVLIKNQQKQ
926

MRSA, S. pneumoniae, E. faecalis,
KSKKKAQ

C. jeikeium

PF-745

S. epidermidis, M. luteus, C. albicans
ANSQNSLFSNRSSFKSIFD
927

KKSNITTNATTPNSNIIIN

PF-746

S. epidermidis, M. luteus, E. coli,
FLGNSQYFTRK
928

C. albicans, S. pneumoniae, E. faecalis,

C. jeikeium

PF-748

S. epidermidis, M. luteus, E. coli,
FQGFFDVAVNKWWEEHN
929

P. aeruginosa, C. albicans,
KAKLWKNVKGKFLEGEG

MRSA, S. pneumoniae, E. faecalis,
EEEDDE

C. jeikeium

PF-749

S. epidermidis, M. luteus, E. coli,
GVNKWWEEHNKAKLWK
930

P. aeruginosa, C. albicans, S. pneumoniae,
NVKGKFLEGEGEEEDDE

C. jeikeium

PF-750

M. luteus, C. jeikeium
AESSPAKTTA
931

PF-751

S. epidermidis, E. coli, C. albicans,
AESSPAQETT
932

C. jeikeium

PF-752

S. epidermidis, E. coli, MRSA, S. pneumoniae,
LHVIRPRPELSELKFPITKI
933

E. faecalis

LKVNKQGLKK

PF-756

S. epidermidis, M. luteus, C. albicans,
DALLRLA
934

MRSA, C. jeikeium

PF-757

M. luteus, C. albicans, MRSA
PQAISSVQQNA
935

PF-758

S. epidermidis, M. luteus, E. coli,
PEIIKIVSGLL
936

MRSA, S. pneumoniae, E. faecalis,

C. jeikeium

PF-760

S. epidermidis, M. luteus
DHITLDDYEIHDGFNFELY
937

YG

PF-761

S. epidermidis, M. luteus, P. mirabilis,
SKFELVNYASGCSCGADC
938

P. aeruginosa, C. albicans,
KCASETECKCASKK

MRSA, S. pneumoniae,

E. faecalis

PF-762

M. luteus, C. albicans
PAPAPSAPAPAPEQPEQPA
939

PF-763

S. epidermidis, M. luteus, E. coli,
GIWMARNYFHRSSIRKVY
940

C. albicans, MRSA, S. pneumoniae,
VESDKEYERVHPMQKIQY

E. faecalis

EGNYKSQ

PF-764

S. epidermidis, M. luteus, C. albicans,
GYFEPGKRD
941

MRSA, S. pneumoniae,

E. faecalis, C. jeikeium

PF-765

S. epidermidis, M. luteus, E. coli,
YLYWEVEHKPIIAKRDAY
942

MRSA, E. faecalis, C. jeikeium
YAQLRKQKEIEEGA

PF-766

S. epidermidis, M. luteus, C. albicans,
DAYYAQLRKQKEIEEGA
943

MRSA, E. faecalis, C. jeikeium

PF-767

S. epidermidis, M. luteus, E. coli,
DGKQGEPVALKPTDN
944

MRSA, S. pneumoniae, E. faecalis,

C. jeikeium

PF-768

S. epidermidis, S. pneumoniae, E. faecalis,
GFRGGKRGGARG
945

C. jeikeium

PF-770

S. epidermidis, M. luteus, P. mirabilis,
GVGIGFIMMGVVGYAVK
946

E. coli, P. aeruginosa,
LVHIPIRYLIV

C. albicans, MRSA, S. pneumoniae,

E. faecalis, C. jeikeium

PF-772

S. epidermidis, C. albicans,
TKESSS
947

MRSA, S. pneumoniae, C. jeikeium

PF-773

S. epidermidis, C. albicans, C. jeikeium
TLKESK
948

PF-776

S. epidermidis, M. luteus, P. mirabilis,
VSILLYLSATIILPNVLRLL
949

C. albicans, MRSA, S. pneumoniae,
VARAIIVRV

E. faecalis

PF-777

Mycobacteria spp.
PGADGKLAEASAAIARLV
950

RS

PF-778

Mycobacteria spp.
MNLILTAHGT
951

PF-779

Mycobacteria spp.
IYGDFFNFYLCDISLKVNG
952

LQPGGPVRTVKLFGQPTG

RCTPQ

PF-780

Mycobacteria spp.
AVYDALVALAAAEHRAE
953

LATRDARAKDTYEKIGVH

VVVAA

PF-781

Mycobacteria spp.
PLVVVNHRRAERSRG
954

PF-782

Mycobacteria spp.
TGPRRGIDLTSNRALSEVL
955

DEGLELNSRK

PF-783

Mycobacteria spp.
FTSEVRGVFTYRVNKAGL
956

ITNMRGYWNLDMMTFGN

QE

PF-784

Mycobacteria spp.
MAMTTVDNIVGLVIAVAL
957

MAFLFAALLFPEKF

PF-785

Mycobacteria spp.
MRPQHSPAGKAFVVKKIT
958

HEQS

PF-786

Mycobacteria spp.
LSERERRRLKRGII
959

PF-787

Mycobacteria spp.
MTERQRRALLKQHPEVVS
960

WSDYLEKRKRRTGTAG

PF-788

Mycobacteria spp.
GLITVFAGTARILQLRRAA
961

KKTHAAALR

PF-789

Mycobacteria spp.
PRGAQSGHG
962

PF-790

Mycobacteria spp.
PAGPDHLDQRDHR
963

PF-791

S. mutans

IFLTTQNTDYSEHNAA
964

PF-792

S. mutans

ALHASGIQAI
965

PF-793

S. mutans

YTQUNNASAYAMLLTNK
966

DTVP

PF-794

S. mutans

NLYFENQGN
967

PF-795

S. mutans

ALHKSGIQVIADWVPDQI
968

YN

PF-796

S. mutans

YTQSNIPTAYALMLSNKD
969

SI

PF-797

S. mutans

WYYFDNNGYM
970

PF-798

S. mutans

ALHSKGIKVMADWVPDQ
971

MYA

PF-799

S. mutans

YTHYNTALSYALLLTNKS
972

SVP

PF-800

S. mutans

WYYFDNNGYM
973

PF-C003

A. naeslundii, P. gingivalis, S. mutans
FCSVDHDVITIAADHVKQ
974

GAEA

PF-C008

A. naeslundii

AQPRRTWLVNFGEVPSPG
975

LTNDGMPDH

PF-C034

S. mutans, E. coli
HPMPITVRSRKPGPLTAPS
976

EH

PF-C045

A. naeslundii, T. denticola
FREGMGWPLSNEGSPTAP
977

LPKHRNQV

PF-C050

A. naeslundii, S. mutans
QGLARPVLRRIPL
978

PF-C052

A. naeslundii, F. nucleatum, S. mutans
SRFRNGV
979

PF-C055

A. naeslundii, F. nucleatum, P. gingivalis,
YNLSIYIYFLHTITIAGLITL
980

S. mutans

PFII

PF-C057

A. naeslundii, F. nucleatum, P. gingivalis,
YFWWYWVQDCIPYKNNE
981

S. mutans

VWLELSNNMK

PF-C058

A. naeslundii, F. nucleatum, P. gingivalis,
FETGFGDGYYMSLWGLN
982

S. mutans

EKDEVCKVVIPFINPELID

PF-C061

A. naeslundii, F. nucleatum, P. gingivalis,
TLNYKKMFFSVIFLLGLN
983

S. mutans, T. denticola
YLICNSPLFFKQIEF

PF-C062

A. naeslundii, F. nucleatum, P. gingivalis,
PLARATEVVATLFIICSLLL
984

S. mutans, T. denticola
YLTR

PF-C063

A. naeslundii, F. nucleatum, S. mutans
SHFRKGD
985

PF-C064

A. naeslundii, F. nucleatum, P. gingivalis,
DEEALEMGANLYAQFAID
986

S. mutans, T. denticola
FLNSKK

PF-C065

A. naeslundii, F. nucleatum, P. gingivalis,
DEERYSDSYFLKEKVFYLI
987

S. mutans

LALFLILFHQKYLYFLEIITI

PF-C068

A. naeslundii, F. nucleatum, S. mutans
LNLFASI
988

PF-C069

A. naeslundii, F. nucleatum, P. gingivalis,
NALMLREMQLAKNIKVE
989

S. mutans, T. denticola
VTDVLSNKKYC

PF-C071

A. naeslundii, F. nucleatum, S. mutans
QVIVKIL
990

PF-C072

A. naeslundii, F. nucleatum, P. gingivalis,
KKMFSLIRKVNWIFFILFIV
991

S. mutans, T. denticola
LDLTNVFPLIRTILFAILSRQ

PF-C075

A. naeslundii, F. nucleatum, P. gingivalis,
KALVISVFAIVFSIIFVKFF
992

S. mutans, T. denticola
YWRDKK

PF-C080

A. naeslundii, F. nucleatum, S. mutans
INIPGLF
993

PF-C084

A. naeslundii, F. nucleatum, P. gingivalis,
FFSVIFLFGLNYLICNSPLF
994

S. mutans

NILR

PF-C085

A. naeslundii, F. nucleatum, P. gingivalis,
KKFKIFVIINWFYHKYIILN
995

S. mutans, T. denticola
FEENF

PF-C086

A. naeslundii, F. nucleatum, P. gingivalis,
ELFFTILSDCNELFLLHLLQ
996

S. mutans, T. denticola
QPLFYIKKGK

PF-C088

A. naeslundii, F. nucleatum, P. gingivalis,
DIANNILNSVSERLIIA
997

S. mutans, T. denticola

PF-C091

A. naeslundii, F. nucleatum, P. gingivalis,
ASNTPRFVRLTLFNFYSKI
998

S. mutans, T. denticola
WNVTHLFLFNNL

PF-C093

A. naeslundii, F. nucleatum, S. mutans
EKLGTMV
999

PF-C095

A. naeslundii, F. nucleatum, P. gingivalis,
LLALNMNEDTYYFELFFIF
1000

S. mutans

DNQNKKWLIFDLKERG

PF-C098

A. naeslundii, F. nucleatum, P. gingivalis,
PETKGKVSAFVFGIVVAN
1001

S. mutans, T. denticola
VIAVVYILYMLREIGIIQ

PF-C120

A. naeslundii, F. nucleatum, P. gingivalis,
ASLSTMTFKVMELKELIIL
1002

S. mutans, T. denticola
LCGLTMLMIQTEFV

PF-C131

A. naeslundii, F. nucleatum, P. gingivalis,
QWIVAKREIRMHIYCHISV
1003

S. mutans

IHVIIFFG

PF-C134

A. naeslundii, F. nucleatum, P. gingivalis,
NELMKYPATLTATATTPG
1004

S. mutans, T. denticola
IKYSHLCSVCL

PF-C135

A. naeslundii, F. nucleatum, P. gingivalis,
KNTHAYLRVLRLSSLILSY
1005

S. mutans

QASVYPLFAYLCQQKDY

PF-C136

A. naeslundii, F. nucleatum, P. gingivalis,
LILSYQASVYPLFAYLCQQ
1006

S. mutans, T. denticola
KDY

PF-C137

A. naeslundii, F. nucleatum, P. gingivalis,
QRMYWFKRGFETGDFSA
1007

S. mutans

GDTFAELK

PF-C139

A. naeslundii, F. nucleatum, P. gingivalis,
LLASHPERLSLGVFFVYRV
1008

S. mutans, T. denticola
LHLLLENT

PF-C142

A. naeslundii, F. nucleatum, P. gingivalis,
DFPPLSFFRRRFHAYTAPI
1009

S. mutans, T. denticola
DNFFGANPF

PF-C143

A. naeslundii, F. nucleatum, P. gingivalis,
VVFGGGDRLV
1010

S. mutans, T. denticola

PF-C145

A. naeslundii, F. nucleatum, P. gingivalis,
YGKESDP
1011

S. mutans, T. denticola

PF-C160

F. nucleatum

AASGFTYCASNGVWHPY
1012

PF-C180

F. nucleatum, P. gingivalis, S. mutans,
TVEELDKAFTWGAAAAL
1013

T. denticola

AIGVIAINVGLAAGYCYN

NNDVF

PF-C181

P. gingivalis

KMRAGQVVFIYKLILVLL
1014

FYVLQKLFDLKKGCF

PF-C194

A. naeslundii, F. nucleatum, P. gingivalis,
NTNDLLQAFELMGLGMA
1015

S. mutans, T. denticola
GVFIVLGILYIVAELLIKIFP

VNN

PF-C259

F. nucleatum, S. mutans
AEIQPHCLSVL
1016

PF-C271

A. naeslundii, F. nucleatum, P. gingivalis,
FFPSYYSIIITYF
1017

S. mutans, T. denticola

PF-C273

A. naeslundii, P. gingivalis, S. mutans,
KNMLKRRMKQKRLFDEE
1018

T. denticola

DRLRVLSKYTKSYY

PF-C281

A. naeslundii, F. nucleatum, P. gingivalis,
KKEKLLTAIRLQHRAEIRG
1019

S. mutans, T. denticola
YFTIFFLFFRI

PF-C285

A. naeslundii, F. nucleatum, P. gingivalis,
FTIIELKKQKIKHGENNKK
1020

S. mutans, T. denticola
TAHPLNEPFCARA

PF-C290

A. naeslundii, F. nucleatum, P. gingivalis,
GNVHPESDFHNLIQFIKTF
1021

S. mutans, T. denticola
LYFTIFFKYFL

PF-C291

A. naeslundii, F. nucleatum, P. gingivalis,
HPFLTGTGCPLFLIFRLFFV
1022

S. mutans

KAYFSFTVF

PF-S003

S. epidermidis, M. luteus, P. mirabilis,
ALALLKQDLLNFEGRGRII
1023

E. coli, P. aeruginosa,
TSTYLQFNEGCVP

C. albicans, MRSA, S. pneumoniae,

E. faecalis, C. jeikeium,

C. jejuni, M. smegmatis

PF-S004

S. epidermidis, MRSA, C. jeikeium
VLLNIFRTLLEFFSPSNAPG
1024

AEDVPLPDTQA

PF-S007

S. epidermidis, MRSA
VVAGVVLLTALAVGSKR
1025

KEKKQIKEIQRLLAATR

PF-S015

S. epidermidis, MRSA, C. jeikeium
IENLERGARRPP
1026

PF-S018

S. epidermidis, M. luteus, C. albicans,
GMPQIPRLRI
1027

MRSA, E. faecalis, C. jeikeium,

C. jejuni

PF-S023

S. epidermidis, MRSA
MAEDERRALKRRTNRGR
1028

TRTRKRITV

PF-S026

S. epidermidis, MRSA, C. jeikeium
TELKYNGEEYLLLTQRDIL
1029

AVIEK

PF-S029

M. luteus, P. mirabilis, E. coli, C. albicans,
TSDTQSQSPWLFDNADIV
1030

C. jeikeium, C. jejuni
NIYPVQLMHSSDND

PF-U

S. mutans

TFFRLFNRSFTQALGK
1031

PF-V

S. mutans

KFINGVLSQFVLERK
1032

PF-W

S. mutans

FIDSFIRSF
1033

PF-X

S. mutans

TFFRLFNRSFTQALGK
1034

PF-Y

S. mutans

FIKHFIHRF
1035

PF-Z

KKHRKHRKHRKH
1036

Additional illustrative suitable targeting peptides include, but are not limited to the peptides shown in Table 10 of copending PCT Patent Application No: PCT/US2010/020242, and Table 3 of copending U.S. Patent Application No. 61/334,511, both of which are incorporated herein by reference. Additional suitable targeting peptides include, but are not limited to, bacterial and/or fungal pheromones such as those shown in Table 12 of PCT Patent Application No: PCT/US2010/020242, which is incorporated herein by reference.

In certain embodiments the targeting moieties can comprise one or more antibodies that bind specifically or preferentially a microorganism or group of microorganisms (e.g., bacteria, fungi, yeasts, protozoa, molds, viruses, algae, etc.). The antibodies are selected to bind an epitope characteristic or the particular target microorganism(s). In various embodiments such epitopes or antigens are typically gram-positive or gram-negative specific, or genus-specific, or species-specific, or strain specific and located on the surface of a target microbial organism. The antibody that binds the epitope or antigen can direct the permeabilizing moiety to the site.

An illustrative, but not limiting list of antibodies that bind various microorganisms is shown in Table 3.

TABLE 3

Illustrative antibodies that bind target microorganisms.

Source
Antibody

U.S. Pat. No. 7,195,763
Polyclonal/monoclonal binds specific Gram(+) cell wall repeats

U.S. Pat. No. 6,939,543
Antibodies against G(+) LTA

U.S. Pat. No. 7,169,903
Antibodies against G(+) peptidoglycan

U.S. Pat. No. 6,231,857
Antibody against S.mutans (Shi)

U.S. Pat. No. 5,484,591
Gram(−) binding antibodies

US 2007/0231321
Diabody binding to Streptococcus surface antigen I/II

US 2003/0124635
Antibody against S.mutans

US 2006/0127372
Antibodies to Actinomycesnaeslundii, Lactobacilluscasei

US 2003/0092086
Antibody to S.sobrinus

U.S. Pat. No. 7,364,738
Monoclonal antibodies to the ClfA protein in S.aureus

U.S. Pat. No. 7,632,502
Antibodies against C.albicans

U.S. Pat. No. 7,608,265
Monoclonal against C.difficile

U.S. Pat. No. 4,777,136
Monoclonal Antibodies against Pseudomonasaeruginosa

see, e.g., ab20429, ab20560,
Antibody against S.pneumoniae

ab79522, ab35165, ab65602

from AbCAMm Cambridge

Science Park, U.K.

Permeabilizing/Lytic Moieties.

In various embodiments the targeting moiety (e.g., targeting antibody or peptide) is attached (directly or indirectly) to a permeabilizing or lytic moiety to produce a selective permeabilizing reagent (i.e., a reagent that selectively permeabilizes a target microorganism, a target group of microorganisms, a target cell, etc.).

Suitable permeabilizing or lytic moieties include, but are not limited to, antimicrobial peptides, surfactants, lytic proteins, cationic colic acid, steroid antibiotics, nanotubes or nanoparticles (e.g., tubes 40 to 400 nm in diameter or particles with a characteristic dimension of typically <500 nm), tubular microtubes (e.g., tubes >400 nm in diameter), carrier proteins or peptides, carrier molecules such as ionophores, lipid flipases, lipases, lysozyme, phage injector assemblies, and the like.

In certain embodiments the permeabilizing or lytic moieties comprise one or more antimicrobial peptides. Illustrative suitable antimicrobial peptides are shown in Table 4.

TABLE 4

Novel antimicrobial peptides,

target microorganisms and MIC values.

Organism MIC

SEQ

ID
(μM)
Structure/sequence
ID NO

K-1

S. mutans, 25
GLGRVIGRLIKQIIWRR
1037

K-2

S. mutans, 12.5
VYRKRKSILKIYAKLKGWH
1038

K-7

S. mutans, 12.5
NYRLVNAIFSKIFKKKFIKF
1039

K-8

S. mutans, 4
KILKFLFKKVF
1040

K-9

S. mutans, 4
FIRKFLKKWLL
1041

K-10

S. mutans, 4
KLFKFLRKHLL
1042

K-11

S. mutans, 4
KILKFLFKQVF
1043

K-12

S. mutans, 8
KILKKLFKFVF
1044

K-13

S. mutans, 16
GILKKLFTKVF
1045

K-14

S. mutans, 8
LRKFLHKLF
1046

K-15

S. mutans, 4
LRKNLRWLF
1047

K-16

S. mutans, 8
FIRKFLQKLHL
1048

P. aeruginosa, 12.5

MRSA, 25

K-17

S. mutans, 8
FTRKFLKFLHL
1049

K-18

S. mutans, 16
KKFKKFKVLKIL
1050

K-19

S. mutans, 16
LLKLLKLKKLKF
1051

K-20

S. mutans, 8
FLKFLKKFFKKLKY
1052

K-21

S. mutans, 8
GWLKMFKKIIGKFGKF
1053

K-22

S. mutans, 8
GIFKKFVKILYKVQKL
1054

1T-88

GRLVLEITADEVKALGEALANAKI
1055

PF-531

A. baumannii, 25
YIQFHLNQQPRPKVKKIKIFL-NH2
1056

P. aeruginosa, 50

T. rubrum, 50

A. niger, 25

B. subtilis, 25

C. difficile, 12.5

C. jeikeium, 6.25

S. epidermidis, 50

S. mutans, 12.5

PF-527

P. aeruginosa, 50
GSVIKKRRKRMAKKKHRKLLKKTRIQR
1057

T. rubrum, 25
RRAGK

A. niger, 50

B. subtilis, 12.5

C. jeikeium, 6.25

MRSA, 50

S. epidermidis, 25

PF-672

C. albicans, 1.56
MRFGSLALVAYDSAIKHSWPRPSSVRR
1058

T. rubrum, 0.78
LRM

A. niger, 3

B. subtilis, 0.78

E. faecalis, 3.13

MRSA, 1.56

S. epidermidis, 0.39

PF-606

E. coli, 50
FESKILNASKELDKEKKVNTALSFNSHQ
1059

MRSA, 50
DFAKAYQNGKI

S. epidermidis, 50

S. mutans, 50

S. pneumoniae, 50

PF-547

T. rubrum, 25
WSRVPGHSDTGWKVWHRW-NH2
1060

B. subtilis, 25

S. mutans, 12.5

PF-006

A. baumannii, 50
MGIIAGIIKFIKGLIEKFTGK
1061

B. subtilis, 25

MRSA, 50

PF-545

A. niger, 50
RESKLIAMADMIRRRI-NH2
1062

B. subtilis, 25

MRSA, 50

PF-278

C. albicans, 50
LSLATFAKIFMTRSNWSLKRFNRL
1063

T. rubrum, 50

S. epidermidis, 50

PF-283

T. rubrum, 50
MIRIRSPTKKKLNRNSISDWKSNTSGRF
1064

B. subtilis, 50
FY

S. epidermidis, 50

PF-307

C. albicans, 50
MKRRRCNWCGKLFYLEEKSKEAYCCK
1065

T. rubrum, 50
ECRKKAKKVKK

B. subtilis, 50

PF-168

T. rubrum, 50
VLPFPAIPLSRRRACVAAPRPRSRQRAS
1066

A. niger, 50

MRSA, 50

PF-538

A. baumannii, 25
KNKKQTDILEKVKEILDKKKKTKSVGQ
1067

C. difficile, 25
KLY

PF-448

A. niger, 25
SLQSQLGPCLHDQRH
1068

S. pneumoniae, 50

PF-583
MRSA, 50
KFQGEFTNIGQSYIVSASHMSTSLNTGK
1069

S. epidermidis, 50

PF-600

E. coli, 50
TKKIELKRFVDAFVKKSYENYILERELK
1070

S. pneumoniae, 50
KLIKAINEELPTK

PF-525

A. niger, 50
KFSDQIDKGQDALKDKLGDL
1071

S. pneumoniae, 50

PF-529

A. niger, 50
LSEMERRRLRKRA-NH2
1072

S. pneumoniae, 50

PF-148

A. niger, 50
RRGCTERLRRMARRNAWDLYAEHFY
1073

B. subtilis, 50

PF-530

A. baumannii, 25
SKFKVLRKIIIKEYKGELMLSIQKQR
1074

PF-522

C. difficile, 25
FELVDWLETNLGKILKSKSA-NH2
1075

PF-497

B. subtilis, 50
LVLRICTDLFTFIKWTIKQRKS
1076

PF-499

B. subtilis, 50
VYSFLYVLVIVRKLLSMKKRIERL
1077

PF-322

B. subtilis, 50
GIVLIGLKLIPLLANVLR
1078

PF-511

S. pneumoniae, 50
VMQSLYVKPPLILVTKLAQQN
1079

PF-512

S. pneumoniae, 50
SFMPEIQKNTIPTQMK
1080

PF-520

S. pneumoniae, 50
LGLTAGVAYAAQPTNQPTNQPTNQPTN
1081

QPTNQPTNQPRW-NH2

PF-521

S. pneumoniae, 50
CGKLLEQKNFFLKTR
1082

PF-523

S. pneumoniae, 50
ASKQASKQASKQASKQASKQASRSLKN
1083

HLL

PF-524

S. pneumoniae, 50
PDAPRTCYHKPILAALSRIVVTDR
1084

PF-209
MRSA, 50
NYAVVSHT
1085

PF-437

S. pneumoniae, 50
FQKPFTGEEVEDFQDDDEIPTII
1086

V

KNLRIIRKGIHIIKKY
1087

W

KLFKFLRKHLL
1088

X

FLKFLKKFFKKLKY
1089

Y

KNLRIIRKGIHIIKKY
1090

Z

KNLRRIIRKGIHIIKKYG
1091

Suitable antimicrobial peptides can include other known antimicrobial peptide sequences. For example, in certain embodiments, the antimicrobial peptides comprise one or more amino acid sequences described in the “Collection of Anti-Microbial Peptides” (CAMP) an online database developed for advancement the understanding of antimicrobial peptides (see, e.g., Thomas et al. (2009) Nucleic Acids Research, 2009, 1-7.doi:10.1093/nar/gkp1021) available at www.bicnirrh.res.in/antimicrobial. Numerous antimicrobial peptides can be found in the antimicrobial peptide database (http://aps.unmc.edu/AP/main.php).

A number of antimicrobial peptides are also disclosed in U.S. Pat. Nos. 7,271,239, 7,223,840, 7,176,276, 6,809,181, 6,699,689, 6,420,116, 6,358,921, 6,316,594, 6,235,973, 6,183,992, 6,143,498, 6,042,848, 6,040,291, 5,936,063, 5,830,993, 5,428,016, 5,424,396, 5,032,574, 4,623,733, which are incorporated herein by reference for the disclosure of particular antimicrobial peptides. In certain embodiments the antimicrobial peptides include any one or more of the peptides disclosed as having antimicrobial activity in PCT Application No: PCT/US2010/020242, which is incorporated herein by reference for the peptides listed therein.

Joining the Lytic/Permeabilizing Moiety to the Targeting Moiety.

The targeting moiety (e.g., targeting peptide, antibody, etc.) can be attached directly to the permeabilizing/lytic moiety or it can be attached by means of one or more linkers. For example, the targeting moiety and the permeabilizing/lytic moiety can be conjugated via a single multifunctional (e.g., bi-, tri-, or tetra-) linking agent or a pair of complementary linking agents. In another embodiment, the targeting moiety and the effector are conjugated via two, three, or more linking agents.

A “linker” or “linking agent” as used herein, is a molecule that is used to join two or more molecules. In certain embodiments the linker is typically capable of forming covalent bonds to both molecule(s) (e.g., the targeting moiety and the effector). Suitable linkers are well known to those of skill in the art and include, but are not limited to, straight or branched-chain carbon linkers, heterocyclic carbon linkers, or peptide linkers.

A bifunctional linker having one functional group reactive with a group on one molecule (e.g., a targeting peptide), and another group reactive on the other molecule (e.g., an antimicrobial peptide), can be used to form the desired conjugate. Alternatively, derivatization can be performed to provide functional groups. Thus, for example, procedures for the generation of free sulfhydryl groups on peptides are also known (see, e.g., U.S. Pat. No. 4,659,839).

In certain embodiments the linking agent is or comprises a functional group. Functional groups include monofunctional linkers comprising a reactive group as well as multifunctional crosslinkers comprising two or more reactive groups capable of forming a bond with two or more different functional targets (e.g., labels, proteins, macromolecules, semiconductor nanocrystals, or substrate). In some embodiments, the multifunctional crosslinkers are heterobifunctional crosslinkers comprising two or more different reactive groups.

Suitable reactive groups include, but are not limited to thiol (—SH), carboxylate (COOH), carboxyl (—COOH), carbonyl, amine (NH₂), hydroxyl (—OH), aldehyde (—CHO), alcohol (ROH), ketone (R₂CO), active hydrogen, ester, sulfhydryl (SH), phosphate (—PO₃), or photoreactive moieties. Amine reactive groups include, but are not limited to e.g., isothiocyanates, isocyanates, acyl azides, NHS esters, sulfonyl chlorides, aldehydes and glyoxals, epoxides and oxiranes, carbonates, arylating agents, imidoesters, carbodiimides, and anhydrides. Thiol-reactive groups include, but are not limited to e.g., haloacetyl and alkyl halide derivates, maleimides, aziridines, acryloyl derivatives, arylating agents, and thiol-disulfides exchange reagents. Carboxylate reactive groups include, but are not limited to e.g., diazoalkanes and diazoacetyl compounds, such as carbonyldiimidazoles and carbodiimides. Hydroxyl reactive groups include, but are not limited to e.g., epoxides and oxiranes, carbonyldiimidazole, oxidation with periodate, N,N′-disuccinimidyl carbonate or N-hydroxylsuccimidyl chloroformate, enzymatic oxidation, alkyl halogens, and isocyanates. Aldehyde and ketone reactive groups include, but are not limited to e.g., hydrazine derivatives for Schiff base formation or reduction amination. Active hydrogen reactive groups include, but are not limited to e.g., diazonium derivatives for mannich condensation and iodination reactions. Photoreactive groups include, but are not limited to e.g., aryl azides and halogenated aryl azides, benzophenones, diazo compounds, and diazirine derivatives.

Other suitable reactive groups and classes of reactions useful in forming chimeric moieties include those that are well known in the art of bioconjugate chemistry. Currently favored classes of reactions available with reactive chelates are those which proceed under relatively mild conditions. These include, but are not limited to, nucleophilic substitutions (e.g., reactions of amines and alcohols with acyl halides, active esters), electrophilic substitutions (e.g., enamine reactions), and additions to carbon-carbon and carbon-heteroatom multiple bonds (e.g., Michael reaction, Diels-Alder addition). These and other useful reactions are discussed in, for example, March (1985) Advanced Organic Chemistry, 3rd Ed., John Wiley & Sons, New York, Hermanson (1996) Bioconjugate Techniques, Academic Press, San Diego; and Feeney et al. (1982) Modification of Proteins; Advances in Chemistry Series, Vol. 198, American Chemical Society, Washington, D.C.

Many procedures and linker molecules for attachment of various molecules to peptides or proteins are known (see, e.g., European Patent Application No. 188,256; U.S. Pat. Nos. 4,671,958, 4,659,839, 4,414,148, 4,699,784; 4,680,338; 4,569,789; and 4,589,071; and Borlinghaus et al. (1987) Cancer Res. 47: 4071-4075).

Where the targeting moiety (e.g., targeting peptide, antibody, etc.) and permeabilizing/lytic moiety are both peptides, the selective permeabilizing reagent can be a fusion protein.

In certain embodiments the chimeric fusion proteins are synthesized using recombinant DNA methodology. Generally this involves creating a DNA sequence that encodes the fusion protein, placing the DNA in an expression cassette under the control of a particular promoter, expressing the protein in a host, isolating the expressed protein and, if required, renaturing the protein.

In other embodiments, the fusion protein can be chemically synthesized.

In certain embodiments a peptide linker/spacer is used to join the one or more targeting moieties the permeabilizing/lytic moiety. In various embodiments the peptide linker is relatively short, typically less than about 10 amino acids, preferably less than about 8 amino acids and more preferably about 3 to about 5 amino acids. Suitable illustrative linkers include, but are not limited to PSGSP ((SEQ ID NO:1092), ASASA (SEQ ID NO: 1093), or GGG (SEQ ID NO: 1094). In certain embodiments longer linkers such as (GGGGS)₃(SEQ ID NO:1095) can be used. Illustrative peptide linkers and other linkers are shown in Table 5.

TABLE 5

Illustrative peptide and non-peptide linkers.

SEQ

Linker
ID NO:

AAA

GGG

SGG

SAT

PYP

ASA

GGSGGS
1096

GGGG
1097

PSPSP
1098

ASASA
1099

PSPSP
1100

KKKK
1101

RRRR
1102

GGGGS
1103

GGGGS GGGGS
1104

GGGGS GGGGS GGGGS
1105

GGGGS GGGGS GGGGS GGGGS
1106

GGGGS GGGGS GGGGS GGGGS GGGGS
1107

GGGGS GGGGS GGGGS GGGGS GGGGS GGGGS
1108

polyethylene glycol (PEG)

2-nitrobenzene or O-nitrobenzyl

Nitropyridyl disulfide

Dioleoylphosphatidylethanolamine (DOPE)

S-acetylmercaptosuccinic acid

1,4,7,10-tetraazacyclododecane-1,4,7,10-

tetracetic acid (DOTA)

β-glucuronide and β-glucuronide variants

Poly(alkylacrylic acid)

Benzene-based linkers (for example:

2,5-Bis(hexyloxy)-1,4-bis[2,5-

bis(hexyloxy)-4-formyl-

phenylenevinylene]benzene) and like

molecules

Disulfide linkages

Poly(amidoamine) or like dendrimers linking

multiple target and killing peptides in one

molecule

Carbon nanotubes

Hydrazone and hydrazone variant linkers

PEG of any chain length

Succinate, formate, acetate butyrate,

other like organic acids

Aldols, alcohols, or enols

Peroxides

alkane or alkene groups of any chain length

One or more porphyrin or dye molecules

containing free amide and

carboxylic acid groups

One or more DNA or RNA nucleotides,

including polyamine and

polycarboxyl-containing variants

Inulin, sucrose, glucose, or other single,

di or polysaccharides

Linoleic acid or other polyunsaturated fatty

acids

Variants of any of the above linkers

containing halogen or thiol groups

(All amino-acid-based linkers could be L, D, combinations of L and D forms, β-form, and the like)

STAMPs as Selective Permeabilization Reagents.

In various embodiments the selective permeabilization reagent is a STAMP (a Specifically Targeted Anti-Microbial Peptide). Typical STAMPs comprise one or more targeting peptides attached to one or more antimicrobial peptides. In certain embodiments the STAMPs are fusion proteins, while in other embodiments, the STAMPs are chemical conjugates.

In certain embodiments suitable STAMPs are contemplated comprising any one or more of the targeting peptides described herein attached directly or through a peptide or non-peptide linker to any one or more of the antimicrobial peptides described herein.

One suitable STAMP for selectively permeabilizing S. mutans is the C16G2 STAMP (SEQ ID NO:1111) which comprises an S. mutans binding peptide TFFRLFNRSFTQALGK (SEQ ID NO:1109) attached to an antimicrobial peptide KNLRIIRKGIHIIKKY (SEQ ID NO:1110).

TABLE 6

Illustrative STAMPs

for use as selective permeabilization reagents.

C-term
SEQ ID

Name
Amino Acid Sequence
modification
NO

Sm-STAMPs

C16G2
TFFRLFNRSFTQALGKGGGKN
optionally
1111

LRIIRKGIHIIKKY
amidated

1845L621
KFINGVLSQFVLERKPYPKLF
optionally
1112

KFLRKHLL
amidated

b43BD2.21
FIDSFIRSFGGGKLFKFLRKH
optionally
1113

LL
amidated

C16AF5
TFFRLFNRSFTQALGKGGGFL
optionally
1114

KFLKKFFKKLKY
amidated

2_1G2
FIKHFIHRFGGGKNLRIIRKG
optionally
1115

IHIIKKY
amidated

Pa-STAMPs

G10KHc
KKHRKHRKHRKHGGSGGSKNL
optionally
1116

RRIIRKGIHIIKKYG
amidated

These STAMPs are intended to be illustrative and not limiting. Using the teachings provided herein methods utilizing numerous other STAMPs that are selectively permeabilizing to S. mutans or other microorganisms will be available to one of skill in the art.

Detection Reagents.

Selection of detection reagent will vary with the format of the assay and/or the metabolite/enzyme (cellular component) that is to be detected. In certain embodiments the detection reagent comprises one or more reagents for the detection of components of a cell (e.g., an enzyme, a metabolite, an ionic species, another intracellular component). Such components include, but are not limited to, ATP, DNA, calcium, beta-galactosidase (beta-gal), beta-glucuronidase, alcohol dehydrogenase or other NAD oxidoreductase, a transferase, an alkaline phosphatase or other hydrolase, a lyase, an isomerase, an oxidase, a gyrase, a nuclease (DNases and RNases), a restriction enzyme, and the like. In certain embodiments, the detection reagent comprises one or more impermeant labels.

Substrates for Enzyme/Metabolite Activity.

Reagents for the detection of cellular components (e.g., enzymes and metabolites), as described above, are well known to those of skill in the art. In certain embodiments, effective detection of permeabilization or lysis of a microorganism can readily be achieved by detecting released ATP. Assays for ATP are well known to those of skill in the art.

A useful assay for detecting ATP released by the selectively permeabilized target microorganism or cell is a luciferase assay. Luciferase assays are based on the use of luciferase in the presence of a luciferase substrate (e.g., luciferin) to produce light (bioluminescence) in the presence of ATP. The light production thus provides a measure of the amount of ATP present in the sample. The luminescence generated by a luciferase reaction is typically detected with a luminometer although other detection means may be used. The presence of light greater than background level indicates the presence of ATP in the sample. The background level of luminescence is typically measured in the same matrix in which the sample exists, but in the absence of the sample. Suitable control reactions are readily designed by one of skill in the art. Luciferase assays for ATP are well known to those of skill in the art are commercially available.

Another method of detecting ATP involves the use of a target-responsive electrochemical aptamer switch (TREAS). In a typical TREAS design for ATP detection, an aptamer oligonucleotide dually labeled with thiol and ferrocene groups is hybridized with its complementary strand, and the thiolated duplex is self-assembled on a an electrode (e.g., a gold electrode). This duplex is responsive to the target ATP, which liberates the complementary strand and forms the aptamer—target complex. The electroactive ferrocene moiety, which is distal to the electrode surface in the absence of ATP, is moved to the proximal position during the binding-induced structural transition. This binding turns on the electron transfer and leads to measurable electrochemical signals for quantification of ATP. Such detection schemes can readily detect ATP at nanomolar levels (see, e.g., Zuo et al. (2007) J. Am. Chem. Soc., 129(5): 1042-1043). Another aptamer-based approach for the detection of ATP utilizes a signaling DNA aptamer attached to silica nanoparticles (see, e.g., Wang et al., (2008) Nanotechnology 19: 415605).

Still another approach to the detection of ATP utilizes a ligase-based ATP electrochemical assay using molecular beacon-like DNA. In this method, biotin-tagged molecular beacon (MB)-like DNA is self-assembled onto an electrode (e.g., a gold electrode) to form a stem-loop structure by means of gold-thiol chemistry, which results in blockage of electronic transmission producing an eT OFF state. In the presence of ATP, two nucleotide fragments which were complementary to the loop of the MB-like DNA can be ligated by ATP-dependent T4 DNA ligase. Hybridization of the ligated DNA with the MB-like DNA induces a significant conformational change in this surface-confined DNA structure, which in turn releases the biotin from the surface allowing free exchange of electrons with the electrode generating a measurable electrochemical signal (eT ON). The resulting change in electron transfer efficiency is readily measured, e.g., by differential pulse voltammetry at target ATP concentrations as low as 0.05 nM and with a linear response range from 0.1 to 1000 nM. This method had been successfully applied to the determination of ATP in the Escherichia coli O157:H7 extracts of water samples, and the linear response was found between the concentrations of 10³and 10⁷cfu/mL (see, e.g., Wang et al. (2009) Biosensors and Bioelectronics, 25(9): 2101-2106).

These approaches to the detection of ATP are illustrative and not intended to be limiting. Numerous other ATP detection schemes are known to those of skill and are commercially available.

In certain embodiments Ca²⁺ion released by the permeabilized cell/microorganism is detected. Numerous fluorogenic or chromogenic indicators for calcium ions are well known to those of skill in the art. Such indicators include, but are not limited to Bis-fura, BTC, Calcium Green-1, Calcium Green-2, Calcium Green-5N, Calcium Orange, Calcium Crimson, Fluo-3, Fluo-4, Fluo-5F, Fluo-4FF, Fluo-5N, Fura-2, Fura-4F, Fura-6F, Fura-FF, Fura Red, Indo-1, Mag-fluo-4, Mag-fura-2, Mag-indo-1, Magnesium Green, Oregon Green 488 BAPTA-1, Oregon Green 488 BAPTA-2, Oregon Green 488 BAPTA-6F, Oregon Green 488 BAPTA-5N, Quin-2, Rhod-2, Rhod-3, Rhod-FF, Rhod-5N, X-rhod-1, and X-rhod-5F.

Similarly, assays for other metabolites, enzymes, and intracellular components (e.g., kinases, phosphatases, lipases, cellulases, etc.) are well known to those of skill in the art. For example, Table 7 lists a few indicators for various enzymatic activities.

TABLE 7

Illustrative indicators for enzyme/metabolite activity.

Detection reagent
Activity Detected

1-Methyl-3-indolyl-β-D-galactopyranoside
Chromogenic substrate for β-galactosidase

that produces a green insoluble product.

2-Acetamido-2-deoxy-3-O-(β-D-
An acceptor for the α1,2-fucosyltransferase

galactopyranosyl)-D-glucopyranose
enzyme from Helicobacter pylori.

2-Ketobutyric acid, sodium salt
Substrate for the determination of lactate

dehydrogenase isoenzymes.

2-Methoxy-4-(2-nitrovinyl)phenyl β-D-
A chromogenic substrate (505 nm) for β-

glucopyranoside
glucosidase.

2-Nitrophenyl b-D-galactopyranoside
A β-galactosidase substrate for colorimetric

and EIA applications; counterpart of widely

employed pNPP/alkaline phosphatase substrate.

o-Nitrophenol is produced as the end product

and is monitored at 405 nm.

3-Indoxyl phosphate, disodium salt
This compound is a histochemical substrate

for alkaline phosphatase.

4-Chloro-1-naphthol
Chromogenic peroxidase substrate that is

useful in enzyme-linked detection

procedures.

4-Methylumbelliferyl butyrate
Suitable to use as a fluorogenic substrate for

esterases/lipases, such as butyrate esterase.

4-Methylumbelliferyl Oleate
Suitable as fluorogenic substrate for lipases.

4-Nitrophenyl acetate
A chromogenic esterase substrate.

4-Nitrophenyl myristate
Suitable as a substrate for lipase.

4-Nitrophenyl phenylphosphat
A 5′-Nucleotide Phosphodiesterase

substrate.

4-Nitrophenyl α-D-maltohexaoside
A substrate used in the determination of α-

amylase activity.

4-Nitrophenyl α-D-xylopyranoside
A chromogenic substrate for α-xylosidase.

5-Bromo-4-chloro-3-indolyl alpha-L-
Employed as a chromogenic substrate for α-

fucopyranoside
D-Fucosidase, producing a blue precipitate.

5-Bromo-4-chloro-3-indoxyl-3-acetate
A histochemical substrate for esterase.

6-Chloro-3-indolyl β-D-glucopyranoside
A substrate used as a chromogenic medium

for the detection of yeasts with β-

glucosidase activity.

8-Hydroxyquinoline-beta-D-glucuronic
A substrate for the histochemical

acid
demonstration of β-glucuronidase and for

quantitative assay systems

Cellotetraose
A substrate for many cellulases and for 1,4-

β-D-glucan glucohydrolases.

Naphthol AS-BI-phosphate, disodium salt
Substrate for the histochemical

hydrate
demonstration of acid and alkaline

phosphatase.

o-Nitropheny1-β-D-xylobioside
A substrate for measuring xylanase activity.

Xylanases are enzymes which hydrolyze

xylan to xylooligosaccharides and have

many applications in the food and feed

industries.

Resorufin acetate
A fluorogenic substrate for hydrolytic

enzymes (cellulases, chymotrypsin).

XGLUC Cyclohexylammonium Salt
Chromogenic substrate for β-glucuronidase

(GUS) gene detection.

Other suitable indicators include, but are not limited to coumarin-4-acetic acid 7-O-caprylate, coumarin-4-acetic acid 7-O-beta-D-glucuronide, and coumarin-4-acetic acid 7-O-beta-D-galactopyranoside.

Reagents for the Detection of Nucleic Acids.

In certain embodiments, selective permeabilization of the target microorganism releases a nucleic acid (e.g., RNA, DNA) which is then detected using a reagent suitable for the detection of nucleic acids. Labeled nucleic acid probes can also introduce another level of specificity and/or selectivity into the assay.

In certain embodiments, nucleic acid(s) released by the permeabilized microorganism is detected using, for example molecular beacons. Molecular beacons are single stranded hairpin shaped oligonucleotide probes labeled with a fluorophore and a quencher (e.g., a fluorescence resonance energy transfer (FRET) system). In the presence of the target sequence, they unfold, bind, the quencher is displaced from the fluorescent moiety, and the beacon fluoresces. In certain embodiments the use of “sloppy molecular beacons” is contemplated (see, e.g., Chakravorty et al. (2010) J. Clin. Microbiol., 48(1): 258-267, for a description of sloppy molecular beacons and their use to detect bacteria).

Released nucleic acids can also be detected using standard well-known PCR methods (e.g., lab-on-a-chip PCR amplification, standard PCR, etc.) with probes designed to amplify nucleic acid(s) from the target organism of interest.

A number of non-PCR based methods can also be used to detect the released nucleic acid(s). Illustrative methods include, but are not limited to the use of strand-displacing polymerases at a constant temperature (e.g., Loop-mediated Isothermal Amplification (LAMP) and Reaction Displacement Chimeric (RDC), or the use of transcription-mediated amplification (e.g., Nucleic acid sequence based amplification (NASBA)). All these methods do not require temperature cycling, operate at a constant temperature, and offer potential advantages including cost, speed, portability and reduced sensitivity to inhibitors over PCR.

Loop-mediated Isothermal Amplification (LAMP), developed by the Eiken Chemical Company is a simple, rapid, specific and cost-effective nucleic acid amplification technology. Details of the method are well known to those of skill in the art (see, e.g., //loopamp.eiken.co.jp/e/lamp/index.html). It is characterized by the use of 4 different primers, specifically designed to recognize 6 distinct regions on the target DNA template, in a process that proceeds at a constant temperature driven by a strand displacement reaction. Amplification and detection of target genes can be completed in a single step, by incubating the mixture of DNA template, primers and a strand displacement DNA polymerase, at a constant temperature. It provides high amplification efficiency, with replication of the original template copy, occurring 10⁹-10¹⁰times during a 15-60 min reaction.

RDC (Reaction déplacement chimeric) is an isothermal DNA amplification procedure developed by Biomerieux, and is based on the use of chimeric primers consisting of an RNA stretch embedded within flanking DNA sequences. Cleavage of the hybrid duplex between the RNA region formed when the primer is hybridized to its DNA target provides the initiation for a strand-displacing polymerase (for details see, e.g., U.S. Pat. No. 5,824,517).

In various embodiments these various technologies can be can be interfaced with a unique reporter system known as BART (bioluminescent assay for real-time). BART is a bioluminescence real time assay developed by Lumora (www.lumora.co.uk) that allows the quantitative analysis of DNA amplification in real time. In BART, PPi produced during DNA amplification is converted to ATP by the action of ATP sulphurylase. This ATP is then used in a coupled simultaneous reaction by thermsotable firefly luciferase and luciferin to produce a light output permitting real-time analysis of amplification kinetics. A unique feature of BART is an initial burst of light, associated with the onset of exponential amplification, followed by a rapid decrease, as pyrophosphate reaches a critical threshold. The time to reach this light peak is therefore a function of the amount of target DNA in the sample at the beginning of the reaction (time to maximum; T_max), and a unique feature of the BART reporter. Quantification of BART is based on time to peak and not absolute light intensity, making it less prone to inhibition simplifying data interpretation and the hardware requirements.

NASBA is an isothermal nucleic acid amplification method that mimics retroviral replication and was originally applied to detection and quantification of RNA targets, but has also been adapted for DNA detection. Amplification occurs because the target is transcribed into RNA, which is then reverse-transcribed back into DNA, thereby providing more template copies for RNA transcription. The transcription is carried out by T7 RNA polymerase and requires the incorporation of the appropriate promoter sequence onto the template, which is achieved by appropriate primer design. This method was modified to allow DNA amplification using a two step procedure: first step with tailed primers, second step with universal primers. NASBA was developed well with performance characteristics similar to PCR, and adaptation to real-time detection using Molecular Beacons has been reported.

Other methods of detecting nucleic acids besides the molecular beacons, labeled probes, PCR, and various alternative amplification strategies described above are known to those of skill in the art.

Moreover, because target specificity is provided by the permeabilization reagent (e.g., STAMP) there is no need for the use of a sequence-specific detection reagent. Thus, for example, nucleic acid(s) can be detected by using labels known to preferentially bind DNA or RNA.

The foregoing detection methods and reagents for the detection of cellular components are meant to be illustrative and not limiting. Using the teachings provided herein detection schemes for other cellular components are readily available to one of skill in the art.

Impermeant Indicator Detection Reagents.

In certain embodiments the uptake of impermeant labels (detection reagents) into the selectively permeabilized target microorganisms, cells, etc. is detected. Impermeant labels are well known to those of skill in the art. Illustrative impermeant labels include, but are not limited to labels such as propidium iodide, SYTOX Green, SYBR®-14, YoYo®-1, YO-PROTM-1, BO-PRO-1, PO-PRO-1, YO-PRO-1, TO-PRO-1, TO-PRO-3, BO-PRO-3, YO-PRO-3, TO-PRO-#, POPO-1, BOBO-1, YOYO-1, TOTO-1, POPO-3, BOBO-2, YOYO-3, TOTO-3, ethidium homodimers-1, ethidium homodimers-2, ethidium bromide, ethidium monoazide, and Trypan blue. In this regard, it is noted that BO stains are benzothiazolium-4-pyridinium dyes, YO stains are benzoxazolium-4-quinolinium dyes, and TO stains are benzothiazolium-4-quinolinium dyes. Such impermeant labels are commercially available (see, e.g., Molecular Probes, Inc., and Invitrogen, Inc.).

It will be appreciated that this list of impermeant labels is not intended to be limiting. Numerous other impermeant labels are known to those of skill in the art an in view of the teachings provided herein it will be recognized that they are suitable in the methods described herein.

Assay Formats and Devices.

The assays described herein can be performed in any of a wide variety of formats that permit detection of one target microorganism, or a plurality of different microorganism and/or evaluation of a single sample, or evaluation of a plurality of different samples. For example, in certain embodiments, different selective permeabilization reagents are located in different reaction chambers (e.g., in a microfluidic device), in different wells (e.g., in a microtiter plate), on different regions of a surface, e.g., in an array format, and the like. In such embodiments, different reaction chambers, wells, regions can be used to assay for different target microorganisms/cells, and the like.

In certain embodiments the assay is provided as a diagnostic test unit. One illustrative diagnostic test is shown in FIG. 6. As schematically shown in this figure the device comprises a swab member 11 carried by a housing base 12 defining a sample chamber 13. The swab member 11 can further comprise a housing cap 14 comprising a first reagent chamber 15 where said housing cap interfits with said housing base 12 to cooperatively form a capped sample chamber 13 with the swab disposed in the sample chamber. The swab member additionally comprises a break-off nib, channel, or port 16 that communicates between the first reagent chamber and the sample chamber. A permeabilization reagent (e.g., a STAMP) 17 that selectively permeabilizes or lyses a target microorganism is disposed within the first reagent chamber 15 or within said sample chamber 13. An optional detection reagent or impermeant label 18 can be disposed within the first reagent chamber 15 when the permeabilization reagent is disposed within the sample chamber 13 or disposed within the sample chamber 13 when the permeabilization reagent is disposed within the first reagent chamber 15. Alternatively, the detection reagent and permeabilization reagent can be disposed within the same chamber, e.g., within the first reagent chamber 15, within the sample chamber 13, or within a second reagent chamber disposed in the housing cap or housing base.

In one usage (as illustrated in FIG. 2), the swab member (e.g., the swab tip is contacted with the sample of interest (e.g., the oral mucosa) to collect a sample. The swab member 11 is then inserted into the housing base 12 where the housing cap interfits with the housing base forming a closed sample chamber 13 with the swab tip 19 disposed therein. The swab can then be allowed to incubate with the permeabilization reagent 17 disposed within the sample chamber 13 to selectively permeabilize target microorganisms that may be present in the sample obtained on the swab tip. After incubation, the housing cap 14 is compressed delivering the detection reagent or impermeant label 18 past the break off nib or through the port or channel 16 into the sample chamber. The detection reagent or impermeant label is allowed to react, with optional mixing of the reaction chamber, to produce a detectable signal, and the signal is read in a test reader.

In certain embodiments the assay comprises a test strip based assay for use in a colorimetric, fluorescent or electrochemical meter. In certain embodiments the test strip is for use in an electrochemical meter.

In general, existing test strips for use in electrochemical meters comprise a substrate, working and reference electrodes formed on the surface of the substrate, and a means for making connection between the electrodes and the meter. The working electrode is coated with an enzyme and/or an enzyme substrate, e.g., as described herein and typically a mediator compound that transfers electrons from the enzyme to the electrode resulting in a measurable current when the target analyte is present. Representative mediator compounds include, but are not limited to a ferricyanide, metallocene compounds such as ferrocene, quinones, phenazinium salts, redox indicator DCPIP, and imidazole-substituted osmium compounds.

In this regard, commercial glucose meters (glucometers) are widely available and easily adapted for use with the test strip assays described herein. A typical glucometer utilizes a test strip comprising an enzyme electrode containing glucose oxidase. The glucose oxidase catalyzes the oxidation of glucose to hydrogen peroxide and D-glucono-δ-lactone in the presence of a cofactor flavin adenine dinucleotide (FAD) which is reduced to FADH₂. Then FADH₂is oxidized by the final electron acceptor, molecular oxygen. The enzyme is reoxidized with an excess of phenol or ferrocyanide ion. The total charge passing through the electrode is measured and is proportional to the concentration of glucose in the blood. The coulometric method is a technique used to define a reaction where the amount of charge measured over a fixed time is measured. The amperometric method is used by some meters that allows the reaction to go to completion and where the total charge transfer is measured.

In one illustrative embodiment, the detection reagent (optionally in and/or on a test strip) an enzyme and a substrate for that enzyme and the detecting involves detecting the reaction between the enzyme and the substrate in the presence of a cofactor or a coenzyme (e.g., FAD, NAD, NADP, ATP, etc.) that is released from the microorganism.

In one illustrative embodiment, the “test strip” comprises glucose or another substrate for glucose oxidase, and glucose oxidase. When the target microorganism is present in the sample, it is lysed/permeabilized by the selective permeabilization reagent releasing one or more coenzymes (e.g., NAD, FAD, NADP). In the presence of the coenzyme, the glucose is oxidized with the corresponding reduction of the coenzyme. The coenzyme is subsequently oxidized and releases electrons (with or without a mediator (e.g. ferrocene, hexacyanoferrate III/hexacyanoferrate II, oxygen/hydrogen peroxide, phenanthroline quinine, nitrosalines, or organic salts such as N-methylphenazinium cation with tetracyanoquinodimethane radical anion). This reaction can be detected directly using a redox color change reagent, a redox fluorescent reagent, or electrochemically. Additional enzymes/enzymatic reactions can be utilized to couple the redox reaction with the detection means.

In another illustrative embodiment, the solid support (test strip) contains hexokinase, a hexose, glucose-6-phosphate dehydrogenase, and NAD. ATP released by the selectively lysed/permeabilized microorganism provides energy to permit the hexokinase to phorphorylate a hexose (e.g. glucose) with the corresponding reduction of NAD to NADH which is then detected directly or with the use of a mediator.

In another illustrative embodiment, the solid support (test strip) comprises glucose-6-phosphate dehydrogenase which in the presence of glucose-6-phosphate reduces NAD to NADH.

In still another illustrative embodiment, the “test strip” contains an alcohol dehydrogenase and an alcohol, and, optionally, a mediator. A coenzyme (e.g., NAD) released from the selectively permeabilized cells permits the reaction between the alcohol and alcohol dehydrogenase to proceed and the reduced NAD is detected.

In a number of embodiments, as indicated above, the detecting comprises detecting released NAD by detecting the reduction of said NAD to NADH.

In certain embodiments wherein the detection of the reduction of NAD, FAD, or NADP e.g., by detection of a colorimetric reagent that changes color when oxidized or reduced, by use of a fluorometric reagent, and/or by electrochemical means (e.g., measurement of impedence, voltage, conductance, current, or charge).

In various embodiments the test strip will carry a calibration code that can be entered into the reading meter, or that can be read directly by the meter. The calibration code can identify the assay chemistry and/or provide a meter calibration.

It will be appreciated that, in various embodiments, the selective permeabilization of the target “cells” can be performed on the test strip, in a sample chamber affixed to the test strip, in a sample collection device, or in a separate reaction chamber.

The materials used in, and the fabrication of enzymatic test strips are well known to those of skill in the art. Examples of glucometers and enzyme test strips are disclosed in European Patent No. 0 127 958, and U.S. Pat. Nos. 5,141,868, 5,286,362, 5,288,636, and 5,437,999 which are incorporated herein by reference.

It will be appreciated that this diagnostic test unit and method of use is intended to be illustrative and not limiting. Using the teachings provided herein numerous diagnostic test units comprising various permeabilization reagents (e.g., STAMPs) as described herein and various detection reagents will be available to one of skill in the art. In addition, using the teachings provided herein, numerous test units known to those of skill and commercially available can readily be adapted to perform the assays described herein (see, e.g., U.S. Pat. No. 5,078,968, U.S. Pat. No. 4,978,504, U.S. Pat. No. 4,707,450, U.S. Pat. No. 5,879,635, U.S. Pat. No. 5,266,266, U.S. Pat. No. 5,238,649, U.S. Pat. No. 5,869,003, U.S. Pat. No. 6,248,294; and U.S. Pat. Nos. 7,892,849, 7,824,344, 7,718,439, 7,556,723, 7,407,811, 7,390,667, 7,115,362, 6,951,728, 6,939,685, 6,923,764, 6,881,578, 6,818,180, 6,800,488, 6,780,651, 6,759,190, 6,638,772, 6,586,199, and 6,572,822, describing test strip, test strip indicators, and test strip meters, and the like).

EXAMPLES

The following examples are offered to illustrate, but not to limit the claimed invention.

Example 1
Detection of S. Mutans Using the Selective Permeability Reagent C16G2 Stamp

A first experiment was performed to determine the detection level of the assay. S. mutans was grown overnight in media and serially diluted to known concentrations in growth media. A 250 μl aliquot of each dilution was mixed with the STAMP (C16G2, SEQ ID NO:1111) and incubated for 10 minutes at room temperature. After incubation the luciferase reagent was added to the dilution, mixed briefly and luminescence measured. The control sample was fresh growth media. As shown in FIG. 3 the assay is capable of quantitatively detecting as little as 10⁴cells/ml of cultured S. mutans grown in the lab. STAMP utilized, C 16G2.

The ability of the assay to detect S. mutans in an unstimulated saliva sample was then determined. The saliva sample came from a volunteer who demonstrated low background levels of native S. mutans. S. mutans was grown overnight in media and serially diluted to known concentrations in the freshly collected unfiltered saliva sample. A 250 μl aliquot of each dilution was mixed with the C16G2 STAMP and incubated for 10 minutes at room temperature. After incubation the luciferase reagent was added to the saliva sample, mixed briefly and luminescence measured. The control sample was fresh saliva. FIG. 4 shows that the assay is capable of quantifying S. mutans spiked in a fresh unfiltered saliva sample. STAMP utilized, C16G2.

An experiment was performed to determine the difference in detection of S. mutans and the non-targeted streptococci, S. sanguinis. The saliva sample came from a volunteer who demonstrated low background levels of native S. mutans. S. mutans and S. sanguinis were grown overnight in media and serially diluted to 10⁷cells/ml in the freshly collected unfiltered saliva sample. A 250 μl aliquot of each dilution was mixed with the Cl₆G2 STAMP and incubated for 10 minutes at room temperature. After incubation the luciferase reagent was added to the saliva sample, mixed briefly and luminescence measured. As shown in FIG. 5, FIG. 3 the assay demonstrates targeted permeabilization of spiked S. mutans in fresh saliva samples. In contrast, the non-target organism (S. sangu9nis) was note permeabilized.

It is understood that the examples and embodiments described herein are for illustrative purposes only and that various modifications or changes in light thereof will be suggested to persons skilled in the art and are to be included within the spirit and purview of this application and scope of the appended claims. All publications, patents, and patent applications cited herein are hereby incorporated by reference in their entirety for all purposes.

	Number	Date	Country
	61446910	Feb 2011	US
	61361463	Jul 2010	US

METHODS AND DEVICES FOR THE SELECTIVE DETECTION OF MICROORGANISMS

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