Claims
- 1. An isolated DNA molecule comprising a DNA sequence coding for a dicamba-degrading oxygenase.
- 2. The DNA molecule of claim 1 comprising a DNA sequence coding for a dicamba-degrading oxygenase having the amino acid sequence of SEQ ID NO: 3.
- 3. The DNA molecule of claim 2 comprising the nucleotide sequence of SEQ ID NO: 2.
- 4. A DNA construct comprising a DNA sequence coding for a dicamba-degrading oxygenase operatively linked to expression control sequences.
- 5. The DNA construct of claim 4 comprising a DNA sequence coding for a dicamba-degrading oxygenase having the amino acid sequence of SEQ ID NO: 3.
- 6. The DNA construct of claim 5 comprising the nucleotide sequence of SEQ ID NO: 2.
- 7. The DNA construct of claim 4 which is a vector.
- 8. An isolated and at least partially purified dicamba-degrading oxygenase.
- 9. The dicamba-degrading oxygenase of claim 8 having the amino acid sequence of SEQ ID NO: 3.
- 10. An isolated DNA molecule comprising a DNA sequence coding for a dicamba-degrading ferredoxin.
- 11. The DNA molecule of claim 10 comprising a DNA sequence coding for a dicamba-degrading ferredoxin having the amino acid sequence of SEQ ID NO: 5.
- 12. The DNA molecule of claim 11 comprising the nucleotide sequence of SEQ ID NO: 4.
- 13. The DNA molecule of claim 11 comprising a nucleotide sequence which has codons selected for optimized expression in dicotyledonous plants.
- 14. A DNA construct comprising a DNA sequence coding for a dicamba-degrading ferredoxin operatively linked to expression control sequences.
- 15. The DNA construct of claim 14 comprising a DNA sequence coding for a dicamba-degrading ferredoxin having the amino acid sequence of SEQ ID NO: 5.
- 16. The DNA construct of claim 15 comprising the nucleotide sequence of SEQ ID NO: 4.
- 17. The DNA construct of claim 15 comprising a nucleotide sequence which has codons selected for optimized expression in dicotyledonous plants.
- 18. The DNA construct of claim 14 which is a vector.
- 19. An isolated and at least partially purified dicamba-degrading ferredoxin.
- 20. The dicamba-degrading ferredoxin of claim 19 which has the amino acid sequence of SEQ ID NO: 5.
- 21. An isolated DNA molecule comprising a DNA sequence coding for a dicamba-degrading reductase.
- 22. The DNA molecule of claim 21 comprising a DNA sequence coding for a dicamba-degrading reductase having the amino acid sequence of SEQ ID NO: 7.
- 23. The DNA molecule of claim 22 comprising the nucleotide sequence of SEQ ID NO: 6.
- 24. The DNA molecule of claim 21 comprising a DNA sequence coding for a dicamba-degrading reductase having the amino acid sequence of SEQ ID NO: 9.
- 25. The DNA molecule of claim 24 comprising the nucleotide sequence of SEQ ID NO: 8.
- 26. A DNA construct comprising a DNA sequence coding for a dicamba-degrading reductase operatively linked to expression control sequences.
- 27. The DNA construct of claim 26 comprising a DNA sequence coding for a dicamba-degrading reductase having the amino acid sequence of SEQ ID NO: 7.
- 28. The DNA construct of claim 27 comprising the nucleotide sequence of SEQ ID NO: 6.
- 29. The DNA construct of claim 26 comprising a DNA sequence coding for a dicamba-degrading reductase having the amino acid sequence of SEQ ID NO: 9.
- 30. The DNA construct of claim 29 comprising the nucleotide sequence of SEQ ID NO: 8.
- 31. The DNA construct of claim 26 which is a vector.
- 32. An isolated and at least partially purified dicamba-degrading reductase.
- 33. The dicamba-degrading reductase of claim 32 which has the amino acid sequence of SEQ ID NO: 7.
- 34. The dicamba-degrading reductase of claim 32 which has the amino acid sequence of SEQ ID NO: 9.
- 35. A DNA construct comprising a DNA sequence coding for a dicamba-degrading oxygenase, a DNA sequence coding for a dicamba-degrading ferredoxin, and a DNA sequence coding for a dicamba-degrading reductase, each DNA sequence being operatively linked to expression control sequences.
- 36. The DNA construct of claim 35 comprising a DNA sequence coding for a dicamba-degrading oxygenase having the amino acid sequence of SEQ ID NO: 3.
- 37. The DNA construct of claim 36 comprising the nucleotide sequence of SEQ ID NO: 2.
- 38. The DNA construct of claim 35 comprising a DNA sequence coding for a dicamba-degrading ferredoxin having the amino acid sequence of SEQ ID NO: 5.
- 39. The DNA construct of claim 38 comprising the nucleotide sequence of SEQ ID NO: 4.
- 40. The DNA construct of claim 38 comprising a nucleotide sequence which has codons selected for optimized expression in dicotyledonous plants.
- 41. The DNA construct of claim 35 comprising a DNA sequence coding for a dicamba-degrading reductase having the amino acid sequence of SEQ ID NO: 7.
- 42. The DNA construct of claim 41 comprising the nucleotide sequence of SEQ ID NO: 6.
- 43. The DNA construct of claim 35 comprising a DNA sequence coding for a dicamba-degrading reductase having the amino acid sequence of SEQ ID NO: 9.
- 44. The DNA construct of claim 43 comprising the nucleotide sequence of SEQ ID NO: 8.
- 45. The DNA construct of claim 35 which is a vector.
- 46. An isolated and at least partially purified dicamba-degrading O-demethylase.
- 47. A transgenic host cell comprising DNA coding for a dicamba-degrading oxygenase operatively linked to expression control sequences.
- 48. The transgenic host cell of claim 47 wherein the DNA codes for a dicamba-degrading oxygenase having the amino acid sequence of SEQ ID NO: 3.
- 49. The transgenic host cell of claim 48 wherein the DNA comprises the nucleotide sequence of SEQ ID NO: 2.
- 50. The transgenic host cell of claim 47 which is a plant cell.
- 51. The transgenic host cell of claim 47 which is a microorganism.
- 52. A transgenic host cell comprising DNA coding for a dicamba-degrading ferredoxin operatively linked to expression control sequences.
- 53. The transgenic host cell of claim 52 wherein the DNA codes for a dicamba-degrading ferredoxin having the amino acid sequence of SEQ ID NO: 5.
- 54. The transgenic host cell of claim 53 wherein the DNA comprises the nucleotide sequence of SEQ ID NO: 4.
- 55. The transgenic host cell of claim 53 wherein the DNA comprises a nucleotide sequence which has codons selected for optimized expression in dicotyledonous plants.
- 56. The transgenic host cell of claim 52 which is a plant cell.
- 57. The transgenic host cell of claim 52 which is a microorganism.
- 58. A transgenic host cell comprising DNA coding for a dicamba-degrading reductase operatively linked to expression control sequences.
- 59. The transgenic host cell of claim 58 wherein the DNA codes for a dicamba-degrading reductase having the amino acid sequence of SEQ ID NO: 7.
- 60. The transgenic host cell of claim 59 wherein the DNA comprises the nucleotide sequence of SEQ ID NO: 6.
- 61. The transgenic host cell of claim 58 wherein the DNA codes for a dicamba-degrading reductase having the amino acid sequence of SEQ ID NO: 9.
- 62. The transgenic host cell of claim 61 wherein the DNA comprises the nucleotide sequence of SEQ ID NO: 8.
- 63. The transgenic host cell of claim 58 which is a plant cell.
- 64. The transgenic host cell of claim 58 which is a microorganism.
- 65. A transgenic host cell comprising a first DNA coding for a dicamba-degrading oxygenase and a second DNA coding for a dicamba-degrading ferredoxin, a third DNA coding for a dicamba-degrading reductase, or both the second DNA and the third DNA, each DNA being operatively linked to expression control sequences.
- 66. The transgenic host cell of claim 65 wherein the first DNA codes for a dicamba-degrading oxygenase having the amino acid sequence of SEQ ID NO: 3.
- 67. The transgenic host cell of claim 66 wherein the first DNA comprises the nucleotide sequence of SEQ ID NO: 2.
- 68. The transgenic host cell of claim 65 wherein the second DNA codes for a dicamba-degrading ferredoxin having the amino acid sequence of SEQ ID NO: 5.
- 69. The transgenic host cell of claim 68 wherein the second DNA comprises the nucleotide sequence of SEQ ID NO: 4.
- 70. The transgenic host cell of claim 68 wherein the second DNA comprises a nucleotide sequence which has codons selected for optimized expression in dicotyledonous plants.
- 71. The transgenic host cell of claim 65 wherein the third DNA codes for a dicamba-degrading reductase having the amino acid sequence of SEQ ID NO: 7.
- 72. The transgenic host cell of claim 71 wherein the third DNA comprises the nucleotide sequence of SEQ ID NO: 6.
- 73. The transgenic host cell of claim 65 wherein the third DNA codes for a dicamba-degrading reductase having the amino acid sequence of SEQ ID NO: 9.
- 74. The transgenic host cell of claim 73 wherein the third DNA comprises the nucleotide sequence of SEQ ID NO: 8.
- 75. The transgenic host cell of claim 65 which is a plant cell.
- 76. The transgenic host cell of claim 65 which is a microorganism.
- 77. A transgenic plant or part of a plant comprising one or more cells comprising DNA coding for a dicamba-degrading oxygenase operatively linked to expression control sequences.
- 78. The transgenic plant or plant part of claim 77 wherein the DNA codes for a dicamba-degrading oxygenase having the amino acid sequence of SEQ ID NO: 3.
- 79. The transgenic plant or plant part of claim 78 wherein the DNA comprises the nucleotide sequence of SEQ ID NO: 2.
- 80. The transgenic plant or plant part of claim 77 wherein the plant is a broadleaf plant which is tolerant to dicamba.
- 81. A transgenic plant or part of a plant comprising one or more cells comprising DNA coding for a dicamba-degrading ferredoxin operatively linked to expression control sequences.
- 82. The transgenic plant or plant part of claim 81 wherein the DNA codes for a dicamba-degrading ferredoxin having the amino acid sequence of SEQ ID NO: 5.
- 83. The trangenic plant or plant part of claim 82 wherein the DNA comprises the nucleotide sequence of SEQ ID NO: 4.
- 84. The trangenic plant or plant part of claim 82 wherein the DNA comprises a nucleotide sequence which has codons selected for optimized expression in dicotyledonous plants.
- 85. The transgenic plant or plant part of claim 81 wherein the plant is a broadleaf plant which is tolerant to dicamba.
- 86. A transgenic plant or part of a plant comprising one or more cells comprising DNA coding for a dicamba-degrading reductase operatively linked to expression control sequences.
- 87. The transgenic plant or plant part of claim 86 wherein the DNA codes for a dicamba-degrading reductase having the amino acid sequence of SEQ ID NO: 7.
- 88. The trangenic plant or plant part of claim 87 wherein the DNA comprises the nucleotide sequence of SEQ ID NO: 6.
- 89. The transgenic plant or plant part of claim 86 wherein the DNA codes for a dicamba-degrading reductase having the amino acid sequence of SEQ ID NO: 9.
- 90. The trangenic plant or plant part of claim 89 wherein the DNA comprises the nucleotide sequence of SEQ ID NO: 8.
- 91. The transgenic plant or plant part of claim 86 wherein the plant is a broadleaf plant which is tolerant to dicamba.
- 92. A transgenic plant or part of a plant comprising one or more cells comprising a first DNA coding for a dicamba-degrading oxygenase and a second DNA coding for a dicamba-degrading ferredoxin, a third DNA coding for a dicamba-degrading reductase, or both the second DNA and the third DNA, each DNA being operatively linked to expression control sequences.
- 93. The transgenic plant or plant part of claim 92 wherein the first DNA codes for a dicamba-degrading oxygenase having the amino acid sequence of SEQ ID NO: 3.
- 94. The transgenic plant or plant part of claim 93 wherein the first DNA comprises the nucleotide sequence of SEQ ID NO: 2.
- 95. The transgenic plant or plant part of claim 92 wherein the second DNA codes for a dicamba-degrading ferredoxin having the amino acid sequence of SEQ ID NO: 5.
- 96. The trangenic plant or plant part of claim 95 wherein the second DNA comprises the nucleotide sequence of SEQ ID NO: 4.
- 97. The trangenic plant or plant part of claim 95 wherein the second DNA comprises a nucleotide sequence which has codons selected for optimized expression in dicotyledonous plants.
- 98. The transgenic plant or plant part of claim 92 wherein the third DNA codes for a dicamba-degrading reductase having the amino acid sequence of SEQ ID NO: 7.
- 99. The transgenic plant or plant part of claim 98 wherein the third DNA comprises the nucleotide sequence of SEQ ID NO: 6.
- 100. The transgenic plant or plant part of claim 92 wherein the third DNA codes for a dicamba-degrading reductase having the amino acid sequence of SEQ ID NO: 9.
- 101. The transgenic plant or plant part of claim 100 wherein the third DNA comprises the nucleotide sequence of SEQ ID NO: 8.
- 102. The transgenic plant or plant part of claim 92 wherein the plant is a broadleaf plant which is tolerant to dicamba.
- 103. A method of controlling weeds in a field containing a dicamba-tolerant transgenic plant, the method comprising applying an amount of dicamba to the field effective to control the weeds in the field.
- 104. A method of decontaminating a material containing dicamba comprising applying an amount of a dicamba-degrading transgenic microorganism to the material, the amount being effective to degrade at least some of the dicamba in the material.
- 105. A method of decontaminating a material containing dicamba comprising applying an amount of a dicamba-degrading O-demethylase or of a combination of a dicamba-degrading oxygenase, a dicamba-degrading ferredoxin and a dicamba-degrading reductase to the material, the amount being effective to degrade at least some of the dicamba in the material.
- 106. A method of selecting transformed plant cells comprising:
providing a population of plant cells; transforming at least some of the plant cells in the population of plant cells so that they are tolerant to dicamba; and growing the resulting population of plant cells in a culture medium containing dicamba at a concentration selected so that transformed plant cells will grow and untransformed plant cells will not grow.
- 107. A method of selecting transformed plants comprising:
providing a population of plants suspected of comprising plants that have been transformed so that they are tolerant to dicamba; and applying an amount of dicamba to the population of plants, the amount of dicamba being selected so that transformed plants will grow and growth of untransformed plants will be inhibited.
- 108. A method of selecting, or screening for, transformed host cells, intact organisms, and parts of organisms, the method comprising:
providing a population of host cells, intact organisms, or parts of organisms suspected of comprising host cells, intact organisms, or parts of organisms that have been transformed so that they are able to degrade dicamba; contacting the population of host cells, intact organisms, or parts of organisms with dicamba; and ascertaining the presence or level of fluorescence due to 3,6-dichlorosalicylic acid, the 3,6-dichlorosalicyclic acid being generated in the host cells, intact organisms, or parts of organisms as a result of the degradation of dicamba.
Parent Case Info
[0001] This application is a continuation-in-part of application Ser. No. 09/055,145, filed Apr. 3, 1998, and claims benefit of provisional applications 60/042,666 and 60/042,941, both filed Apr. 4, 1997, the complete disclosures of all three of which are incorporated herein by reference.
Provisional Applications (2)
|
Number |
Date |
Country |
|
60042666 |
Apr 1997 |
US |
|
60042941 |
Apr 1997 |
US |
Divisions (1)
|
Number |
Date |
Country |
Parent |
09797238 |
Feb 2001 |
US |
Child |
10330661 |
Dec 2002 |
US |
Continuation in Parts (1)
|
Number |
Date |
Country |
Parent |
09055145 |
Apr 1998 |
US |
Child |
09797238 |
Feb 2001 |
US |