Claims
- 1. A nanotextured biocompatible composite, comprising a biocompatible substrate, a calcium phosphate component on such said substrate; and a nanotextured mineral phase on said calcium phosphate component, said mineral phase comprising calcium phosphate and poly(L-lysine).
- 2. The composite of claim 1 wherein the calcium content of said mineral phase is less than stoichiometric, and said poly(L-lysine) is incorporated within said calcium phosphate.
- 3. The composite of claim 1 wherein said mineral phase is reactive with at least one of an acid and degradative enzyme.
- 4. The composite of claim 1 further comprising nanofibers of peptide amphiphiles coupled to said poly(L-lysine), at least one of said peptide amphiphiles comprising a carboxy functionality.
- 5. The composite of claim 4 wherein at least one of said peptide amphiphiles comprises an RGD sequence.
- 6. The composite of claim 4 further comprising a mammalian preosteoblast cell culture.
- 7. The composite of claim 1 wherein said substrate comprises titanium.
- 8. A method of promoting growth of an amine-modified calcium phosphate composition, said method comprising:
providing a biocompatible substrate; depositing a substantially single-phase calcium phosphate component on said substrate; and introducing said substrate to a calcium phosphate medium, said medium comprising a poly(L-lysine) component.
- 9. The method of claim 7 wherein said substrate contacts a medium comprising a reactive calcium reagent and a reactive phosphate reagent, said contact for a time sufficient to deposit said calcium phosphate component on said substrate.
- 10. The method of claim 8 wherein said calcium phosphate medium comprises at least one of a reactive calcium reagent and a reactive phosphate reagent.
- 11. The method of claim 10 wherein at least one of said reagents comprises said poly(L-lysine) component.
- 12. The method of claim 8 wherein said deposition comprises formation of crystalline calcium phosphate, and said introduction incorporates poly(L-lysine) into a calcium phosphate phase.
- 13. The method of claim 12 wherein said introduction induces a nanotextured component comprising calcium phosphate and poly(L-lysine).
- 14. A method of coupling peptide amphiphiles to a biocompatible substrate, said method comprising:
providing a biocompatible substrate; depositing a substantially single-phase calcium phosphate component on said substrate; depositing a mineral phase on said calcium phosphate phase, said mineral phase comprising calcium phosphate and poly(L-lysine) incorporated therein; and contacting said poly(L-lysine) with peptide amphiphiles, at least one of said amphiphiles comprising a carboxy functionality.
- 15. The method of claim 14 wherein said peptide amphiphiles comprise a nanofiber assembly.
- 16. The method of claim 14 wherein said substrate contacts a medium comprising a reactive calcium reagent and a reactive phosphate reagent, said contact for a time sufficient to deposit said calcium phosphate component on said substrate.
- 17. The method of claim 14 wherein said mineral phase is the reaction product of a calcium reagent and a phosphate reagent, and introduction of poly(L-lysine) during said reaction.
- 18. The method of claim 14 further comprising contacting said mineral phase with at least one of an acid and a degradative enzyme.
- 19. The method of claim 14 wherein at least one of said peptide amphiphiles comprises an RGD sequence.
- 20. The method of claim 14 further comprising, culturing mammalian cells on said peptide amphiphiles.
Parent Case Info
[0001] This application claims priority benefit from U.S. provisional applications Ser. Nos. 60/446,421 and 60/495,965 filed Feb. 11, 2003 and Aug. 18, 2003, respectively, each of which is incorporated herein by reference in its entirety.
Government Interests
[0002] The United States Government has certain rights to this invention pursuant to grant No. DEFG02-00ER45810 and DMR0108342 from the Department of Energy and the National Science Foundation, respectively, to Northwestern University.
Provisional Applications (2)
|
Number |
Date |
Country |
|
60446421 |
Feb 2003 |
US |
|
60495965 |
Aug 2003 |
US |