Patent Application
20180078599
This document relates to methods and materials for treating cancer. For example, this document relates to using compositions containing a potato polysaccharide preparation to reduce the number of cancer cells in a mammal. In some cases, this document relates to using compositions containing a potato polysaccharide preparation to reduce the number of cancer cells in a mammal, wherein the cancer cells express a v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog (KRAS) polypeptide.
Potatoes are starchy, edible tubers obtained from potato plants and form an integral part of much of the world's food supply. In fact, potatoes are the fourth largest food crop in the world. The main potato species worldwide is Solanum tuberosum.
This document provides methods and materials for treating cancer. For example, this document provides methods for using compositions containing a potato polysaccharide preparation to reduce the number of cancer cells in a mammal. In some cases, a composition containing a potato polysaccharide preparation provided herein can be used to reduce the number of cancer cells in a mammal, wherein the cancer cells express a KRAS polypeptide.
Having the ability to use a composition containing a potato polysaccharide preparation described herein to reduce the number of cancer cells in a mammal can provide clinicians and patients with an effective treatment regime for cancer.
This document also provides compositions (e.g., nutritional supplement compositions) that contain a potato polysaccharide preparation. For example, this document provides nutritional supplement compositions containing a potato polysaccharide preparation, methods for obtaining potato polysaccharide preparations, methods for making nutritional supplement compositions containing a potato polysaccharide preparation, and methods for increasing or decreasing expression of polypeptides involved with cancer.
In some cases, a composition containing a potato polysaccharide preparation provided herein can be used to decrease expression of a KRAS polypeptide and/or an oncogene polypeptide functionally interrelated with a KRAS polypeptide.
In some cases, the compositions provided herein (e.g., a nutritional supplement composition or a potato polysaccharide preparation provided herein) can be used to increase or decrease expression of polypeptides involved with cancer. For example, a composition containing a potato polysaccharide preparation provided herein or a potato polysaccharide preparation provided herein can be used to decrease expression of a KRAS polypeptide, a soc-2 suppressor of clear homolog (SOC2) polypeptide, an integrin-linked protein kinase (ILK) polypeptide, a heat shock 70 kDa protein (HSP9A) polypeptide, or a combination thereof.
In general, one aspect of this document features a method for reducing the number of cancer cells in a mammal. The method comprises, or consists essentially of, (a) identifying a mammal having cancer cells that express a KRAS polypeptide, and (b) administering to the mammal a composition comprising a potato polysaccharide preparation obtained from raw potatoes, wherein the number of cancer cells in the mammal is reduced. The composition can reduce expression of a KRAS polypeptide. The composition can reduce expression of a SHOC2 polypeptide, an ILK polypeptide, or a HSP9A polypeptide. The cancer cells can be colorectal cancer cells, non-small-cell lung cancer cells, pancreatic cancer cells, liver cancer cells, or neuroblastoma cancer cells. The mammal can be a human. The composition can further comprise a chemotherapeutic agent. The chemotherapeutic agent can be selected from the group comprising anti-PD-1 antibodies, anti-PD-L1 antibodies, anti-CTLA4 antibodies, Herceptin, cyclophosphamide, gemcitabine, capecitabine, azacytadine, bortezomib, carboplatin, cisplatin, etoposide, imatinib, 5-fluorouracil/leucovorin, docetaxel, paclitaxel, nab-paclitaxel, irinotecan, doxorubicin, methotrexate, and oxaliplatin therapies. The composition can comprise the potato polysaccharide preparation in an amount that results in between 0.05 mg and 50 mg of the potato polysaccharide component of the potato polysaccharide preparation being administered to the mammal per kg of body weight of the mammal. The composition can comprise between 1 mg and 100 mg of the potato polysaccharide preparation. The composition can comprise between 6 mg and 20 mg of the potato polysaccharide preparation. The composition can comprise between 1 mg and 100 mg of the potato polysaccharide component of the potato polysaccharide preparation. The composition can comprise between 6 mg and 20 mg of the potato polysaccharide component of the potato polysaccharide preparation. The composition can be in the form of a tablet. The composition can comprise alpha lipoic acid. The composition can comprise alpha tocopherol. The potato polysaccharide preparation can be in an amount that results in between 0.075 mg and 0.5 mg of the potato polysaccharide component of the potato polysaccharide preparation being administered to the mammal per kg of body weight of the mammal. At least about 80 percent of the potato polysaccharide preparation can be potato polysaccharide. At least about 90 percent of the potato polysaccharide preparation can be potato polysaccharide. At least about 95 percent of the potato polysaccharide preparation can be potato polysaccharide.
Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains. Although methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present invention, suitable methods and materials are described below. All publications, patent applications, patents, and other references mentioned herein are incorporated by reference in their entirety. In case of conflict, the present specification, including definitions, will control. In addition, the materials, methods, and examples are illustrative only and not intended to be limiting.
Other features and advantages of the invention will be apparent from the following detailed description, and from the claims.
This document provides methods and materials for treating cancer. For example, this document provides methods for using compositions containing a potato polysaccharide preparation to reduce the number of cancer cells in a mammal. As described herein, a composition containing a potato polysaccharide preparation provided herein (e.g., a nutritional supplement composition provided herein) can be administered to any appropriate mammal to reduce the number of cancer cells within the mammal, to reduce tumor growth within the mammal, to increase survival time of the mammal, and/or to reduce the likelihood of metastasis within the mammal.
In some cases, this document provides methods and materials related to treating mammals (e.g., humans) having cancer. Examples of mammals that can be treated as described herein include, without limitation, humans, monkeys, dogs, cats, cows, horses, pigs, ducks, rabbits, sheep, rats, and mice. Examples of cancers that can be treated as described herein include, without limitation, colorectal cancers, pancreatic cancers, non-small-cell lung cancers, liver cancers, or neuroblastoma cancers. A mammal can be identified as having cancer using any appropriate cancer diagnostic techniques.
In some cases, the compositions provided herein (e.g., nutritional supplement compositions and potato polysaccharide preparations provided herein) can be used alone or in combination with chemotherapy and/or anti-cancer immunotherapy to treat cancer or to reduce the number of cancer cells within a mammal. Examples of chemotherapeutic agents that can be used in combination with the compositions provided herein (e.g., a nutritional supplement composition or a potato polysaccharide preparation provided herein) to treat cancer or to reduce the number of cancer cells within a mammal as described herein include, without limitation, anti-PD-1 antibodies, anti-PD-L1 antibodies, anti-CTLA4 antibodies, Herceptin, cyclophosphamide, gemcitabine, capecitabine, azacytadine, bortezomib, carboplatin, cisplatin, etoposide, imatinib, 5-fluorouracil/leucovorin, docetaxel, paclitaxel, nab-paclitaxel, irinotecan, doxorubicin, methotrexate, and oxaliplatin therapies.
Any appropriate route of administration (e.g., oral or parenteral administration) can be used to administer a composition containing a potato polysaccharide preparation provided herein (e.g., a nutritional supplement composition provided herein) to a mammal. For example, a composition containing a potato polysaccharide preparation provided herein can be administered orally. In some cases a composition containing a potato polysaccharide preparation provided herein can be administered by injection.
A composition provided herein (e.g., a nutritional supplement composition) can include one or more potato polysaccharide preparations. A potato polysaccharide preparation can be a preparation that is obtained from a water extract of potato and that contains polysaccharide material having the ability to be eluted from a C18 cartridge (e.g., a Sep-Pak Plus C-18 cartridge) with 10% acetonitrile. In some cases, a potato polysaccharide preparation can be a preparation that is obtained from potato and that contains polysaccharide material having HPLC characteristics of that of the peak eluted at 3.5 minutes as described in Example 1 (see, also,
In some cases, a potato polysaccharide preparation can be a preparation that is obtained from potato and that contains polysaccharide material that, when derivatized, results in at least the following acylated carbohydrates as assessed using GC/MS: (a) myo-inositol (set to 1× to serve as an internal standard), (b) glucose at about 40× to about 60× the myo-inositol content (e.g., glucose at about 50× the myo-inositol content), (c) xylose at about 10× to about 20× the myo-inositol content (e.g., xylose at about 15× the myo-inositol content), (d) mannose at about 5× to about 15× the myo-inositol content (e.g., mannose at about 10× the myo-inositol content), and (e) galactose at about 3× to about 7× the myo-inositol content (e.g., galactose at about 5× the myo-inositol content). The derivatization procedure can include forming a dry residue of the polysaccharide material that is then hydrolyzed using trifluoroacetic acid. The resulting material is then reduced using sodium borohydride, and after borate removal, the end product is acylated using acetic anhydride and pyridine. The end products of the reaction are then injected directly on GC/MS to identify the acylated carbohydrates.
In some cases, a potato polysaccharide preparation can be a preparation that is obtained from potato and that contains polysaccharide material that, when derivatized and assessed using GC/MS, results in at least four major components (3,4-furan dimethanol, diacetate; 1,2,3,4,5-penta-o-acetyl-D-xylitol (isomer 1); 3,5-diacetoxy-benzyl alcohol; and D-glucitol-hexaacetate). See, e.g., Example 1. In some cases, a potato polysaccharide preparation can be a preparation that is obtained from potato and that contains polysaccharide material that, when derivatized and assessed using GC/MS, results in the compounds listed in Table 1 or results in the profile shown in
In some cases, a potato polysaccharide preparation provided herein can be a substantially pure potato polysaccharide preparation. Typically, a substantially pure potato polysaccharide preparation is a preparation that contains a single peak of material (e.g., a single peak of polysaccharide material) when assessed using, for example, HPLC (see, e.g.,
Any appropriate potato species or variety can be used to obtain a potato polysaccharide preparation provided herein. For example, Solanum tuberosum, Ipomoea batatas, S. acaule, S. bukasovii, S. leptophyes, S. megistacrolobum, S. commersonii, or S. infundibuliforme can be used to obtain a potato polysaccharide preparation provided herein. In some cases, potato varieties of S. tunerosum such as Organic Yellow, Purple or blue varieties, Cream of the Crop, Adirondack Blue, Adirondack Red, Agata, Almond, Andes Gold, Andes Sun, Apline, Alturas, Amandine, Annabelle, Anya, Arran Victory, Atlantic, Avalanche, Bamberg, Bannock Russet, Belle de Fontenay, BF-15, Bildtstar, Bintje, Blazer Russet, Blue Congo, Bonnotte, British Queens, Cabritas, Camota, Canela Russet, Cara, Carola, Chelina, Chiloé, Cielo, Clavela Blanca, Desiree, Estima, Fianna, Fingerling, Flava, German Butterball, Golden Wonder, Goldrush, Home Guard, Innovator, Irish Cobbler, Jersey Royal, Kennebec, Kerr's Pink, Kestrel, Keuka Gold, King Edward, Kipfler, Lady Balfour, Langlade, Linda, Marcy, Marfona, Maris Piper, Marquis, Megachip, Monalisa, Nicola, Pachacona, Pike, Pink Eye, Pink Fir Apple, Primura, Ranger Russet, Ratte, Record, Red LaSoda, Red Norland, Red Pontiac, Rooster, Russet Burbank, Russet Norkotah, Selma, Shepody, Sieglinde, Silverton Russet, Sirco, Snowden, Spunta, Up to date, Stobrawa, Superior, Vivaldi, Vitelotte, Yellow Finn, or Yukon Gold can be used to obtain a potato polysaccharide preparation provided herein.
Any appropriate method can be used to obtain a potato polysaccharide preparation provided herein. For example, raw potato material can be homogenized (e.g., homogenized with a Polytron homogenizer) in water and maintained at room temperature for a period of time (e.g., about 1 hour) with occasional shaking. The homogenate can be centrifuged (e.g., centrifuged at 4000 g for 10 minutes) to remove any larger solid material. The resulting supernatant can be loaded onto a Solid Phase Extraction cartridge (e.g., a C18 cartridge such as a Sep-Pak Plus C-18 cartridge), and the polysaccharide material eluted with 10 percent acetonitrile. Once eluted, the polysaccharide material can be dried and stored (e.g., stored at about 4° C.).
This document also provides nutritional supplement compositions containing one or more potato polysaccharide preparations provided herein. For example, a potato polysaccharide preparation provided herein obtained from Idaho Russet potatoes can be formulated into a nutritional supplement composition.
Any appropriate dose of a potato polysaccharide preparation provided herein can be used to formulate a composition provided herein (e.g., a nutritional supplement composition or potato polysaccharide preparation provided herein). For example, a potato polysaccharide preparation provided herein can be used to formulate a composition for reducing the number of cancer cells within a mammal having cancer cells that express a KRAS polypeptide. The composition can contain between about 1 mg and about 750 mg (e.g., between about 1 mg and about 500 mg, between about 1 mg and about 250 mg, between about 5 mg and about 40 mg, between about 5 mg and about 30 mg, between about 5 mg and about 20 mg, between about 6 mg and about 50 mg, between about 6 mg and about 20 mg, between about 10 mg and about 25 mg, or between about 15 mg and about 20 mg) of the potato polysaccharide component of the potato polysaccharide preparation. In some cases, a composition (e.g., a nutritional supplement composition) can be formulated to deliver about 0.05 mg of the potato polysaccharide component per kg of body weight to about 0.5 mg of the potato polysaccharide component per kg of body weight to a mammal (e.g., a human) per day. For example, a nutritional supplement composition can be formulated into a single oral composition that a human can swallow once a day to provide between about 0.05 mg of the potato polysaccharide component per kg of body weight to about 0.5 mg of the potato polysaccharide component per kg of body weight.
Any appropriate method can be used to formulate a composition provided herein (e.g., a nutritional supplement composition or potato polysaccharide preparation provided herein). For example, common formulation mixing techniques and preparation techniques can be used to make a composition (e.g., a nutritional supplement composition) having the components described herein. In addition, a composition provided herein (e.g., a nutritional supplement composition or potato polysaccharide preparation provided herein) can be in any form. For example, a composition provided herein (e.g., a nutritional supplement composition or potato polysaccharide preparation provided herein) can be formulated into a pill, capsule, tablet, gel cap, nutritional shake, nutritional bar, rectal suppository, sublingual suppository, nasal spray, inhalant, or injectable ampule. In some cases, a composition provided herein (e.g., a nutritional supplement composition) can include one or more potato polysaccharide preparations provided herein alone or in combination with other ingredients including, without limitation, gelatin, cellulose, starch, sugar, bentonite, lactic acid, mannitol, alpha lipoic acid, alpha tocopherol, L-ascorbate, or combinations thereof.
As described herein, a composition containing a potato polysaccharide preparation provided herein (e.g., a nutritional supplement composition or a potato polysaccharide preparation provided herein) can be used to increase or decrease expression of a KRAS polypeptide and/or a polypeptide involved with cancer. For example, a composition containing a potato polysaccharide preparation provided herein or a potato polysaccharide preparation provided herein can be used to decrease expression of a KRAS polypeptide, a SOC2 polypeptide, an ILK polypeptide, an HSP9A polypeptide, or a combination thereof.
In some cases, a composition provided herein can be used to decrease expression of a KRAS polypeptide by about 5% to about 70% (e.g., from about 10% to about 70%, from about 15% to about 70%, from about 20% to about 70%, from about 5% to about 45%, from about 5% to about 60%, from about 5 to about 50%, from about 15% to about 40%, or from about 20% to about 30%). In some cases, a composition provided herein can be used to decrease expression of a SOC2 polypeptide by about 5% to about 50% (e.g., from about 10% to about 50%, from about 15% to about 50%, from about 20% to about 50%, from about 5% to about 45%, from about 5% to about 40%, from about 5 to about 35%, from about 15% to about 30%, or from about 20% to about 40%). In some cases, a composition provided herein can be used to decrease expression of an ILK polypeptide by about 5% to about 50% (e.g., from about 10% to about 50%, from about 15% to about 50%, from about 20% to about 50%, from about 5% to about 45%, from about 5% to about 40%, from about 5 to about 35%, from about 15% to about 30%, or from about 20% to about 40%). In some cases, a composition provided herein can be used to decrease expression of a HSPA9 polypeptide by about 5% to about 50% (e.g., from about 10% to about 50%, from about 15% to about 50%, from about 20% to about 50%, from about 5% to about 45%, from about 5% to about 40%, from about 5 to about 35%, from about 15% to about 30%, or from about 20% to about 40%).
In humans, a composition containing a potato polysaccharide preparation provided herein or a potato polysaccharide preparation provided herein can be used to decrease expression of a human KRAS polypeptide, a human SOC2 polypeptide, a human ILK polypeptide, a human HSPA9 polypeptide, or a combination thereof.
A human KRAS polypeptide can have the amino acid sequence set forth in GenBank® Accession No. NP_203524.1 (GI No. 15718763) and can be encoded by the nucleic acid sequence set forth in GenBank® Accession No. NP_033360.2 (GI No. 34485724). In some cases, a human KRAS polypeptide can have the amino acid sequence set forth in GenBank® Accession No. NM_004976.2 (GI No. 15718761) and can be encoded by the nucleic acid sequence set forth in GenBank® Accession No. NM_004985.3 (GI No. 34485723). In some cases, a human KRAS polypeptide can have the amino acid sequence set forth in GenBank® Accession No. XP_005253422.1 (GI No. 530399133) and can be encoded by the nucleic acid sequence set forth in GenBank® Accession No. XM_005253365.1 (GI No. 530399132). A human SOC2 polypeptide can have the amino acid sequence set forth in GenBank® Accession No. NP_031399.2 (GI No. 41281398) and can be encoded by the nucleic acid sequence set forth in GenBank® Accession No. NM_001269039.1 (GI No. 392841223). A human ILK polypeptide can have the amino acid sequence set forth in GenBank® Accession No. CAG28601.1 (GI No. 47115283) and can be encoded by the nucleic acid sequence set forth in GenBank® Accession No. NM_001014794.2 (GI No. 510785737). A human HSP9A polypeptide, can have the amino acid sequence set forth in GenBank® Accession No. NP_004125.3 (GI No. 24234688) and can be encoded by the nucleic acid sequence set forth in GenBank® Accession No. NG_029469 (GI No. 340523104).
The document will provide addition description in the following examples, which do not limit the scope of the invention described in the claims.
6 grams of a Russet potato variety of the Solanum tuberosum species were homogenized with a Polytron homogenizer in 20 mL water in a 50 mL centrifuge tube and kept at room temperature for 1 hour. The homogenate was centrifuged at 4000 rpm for 10 minutes. A Sep-Pak Plus C-18 cartridge was activated with 10 mL 100% acetonitrile (ACN) and washed with 10 mL 0.05% trifluoroacetic acid in water (TFA water). 10 mL of the supernatant was loaded onto the cartridge, and all H2O that passes through cartridge was collected in 1.5 mL Eppendorf tubes. Next, 10 mL of 2% ACN (in 0.05% TFA water) was passed through the column, and the elutriate was collected in 1.5 mL Eppendorf tubes. Next, 10 mL of 5% ACN (in 0.05% TFA water) was used to wash the column, and the elutriate was collected in 1.5 mL Eppendorf tubes. Finally, 10 mL of 10% ACN (in 0.05% TFA water) was collected in 1.5 mL Eppendorf tubes after passing through the column. All of the fractions were dried, and the dried fractions of the same ACN concentration were reconstituted into 1 tube in 1 mL of 0.05% TFA water for further purification via HPLC or reconstituted in 1 mL of phosphate buffered saline for use in cell treatments.
A Waters 2695 separations module with a photodiode array detector was used to purify the 10% ACN extract. An XterraRP C18 column (4.6×150 mm) was used for the separation with 0.05% TFA water as the mobile phase. Each HPLC run was a 20 minute gradient ranging from 0 to 2.5% ACN. The injection volume was 100 μL, and the flow rate was 0.5 mL/minute. HPLC fractionation of the 10% ACN extract yielded three major UV absorbing peaks eluted at 3.5, 3.9, and 12.1 minutes (
Further chemical characterization of the symmetrical 3.5 minute HPLC peak material was performed. Pooled 3.5 minute HPLC fractions were dried and reconstituted in 1 mL TFA water and subjected to tandem LC/MS/MS (
Further analysis confirmed that the HPLC purified fraction eluting at 3.5 minutes contains polysaccharide material (e.g., highly substituted complex xyloglucan material). To make the polysaccharide material analyzable by gas chromatography/mass spectroscopy (GC/MS), it was converted into its derivatized carbohydrate fragments. Briefly, the sample was concentrated to a dry residue that was hydrolyzed using trifluoroacetic acid. This was then reduced using sodium borohydride, and after borate removal, the end product was acylated using acetic anhydride and pyridine. The end products of the reaction were injected directly on GC/MS to identify any acylated carbohydrates. Based on the end analysis, a larger carbohydrate existed in the sample. The total ion chromatogram (TIC) is shown below in
These results demonstrate the presence of sugar monomers that serve as building blocks for a larger carbohydrate. It appeared from these multiple lines of analysis that the potato polysaccharide preparation is a highly substituted complex xyloglucan.
Six grams of potato material from multiple varieties of Solanum tuberosum (Organic yellow, Purple, Idaho Russet, and Yukon Gold) and six grams of material from sweet potatoes (Ipomoea batatas) were extracted in 20 mL of water. 10 mL of that water was then loaded onto a sep-pak cartridge, and the cartridge was then eluted with 10 mL of 10% ACN. The ACN was then dried, and the residue was dissolved in 1 mL of water. A 100 μL injection of this water was assessed using HPLC.
The HPLC chromatograms demonstrated that the amount of the first peak (at 3.5 minutes at 210 nm) was the same for all five types of potatoes tested (
In another experiment, material was extracted from a boiled Purple potato and analyzed. The peak at 3.5 minutes was not reduced in the boiled potato (
The 3.5 minute peak from two pooled fraction collections from Idaho Russet potatoes was collected, dried, and reconstituted in 100 μL of water. The material was then injected into the HPLC yielding a single peak at 3.5 minutes (
A potato polysaccharide preparation was purified using HPLC from 3 g of purple potato. The potato polysaccharide peak was eluted at about 5 minutes (
To determine the effects of potato polysaccharide preparation administration on the expression of oncogenic KRAS and potentially interactive oncogenes in an established neuroblastoma cell line, HTB-11 neuroblastoma cells obtained from American Type Culture Collection (ATCC) were plated at a concentration of 5×105 cells/2 mL into each well of 6-well culture plates using standard culture media. In separate incubations, HTB-11 neuroblastoma cells were administered purified potato polysaccharide preparation at a final concentration of 60 μg/mL or potato polysaccharide preparation vehicle for 4 hours. In vitro potato polysaccharide preparation trials were performed in triplicate.
Typically, 6 g of potato were homogenized with a Polytron homogenizer in 20 mL water in a 50 mL centrifuge tube and kept at room temperature for 1 hour. The homogenate was centrifuged at 4000 rpm for 10 minutes and the supernatant fraction was reserved. 10 mL of the supernatant fraction was percolated through a Sep-Pak Plus C-18 cartridge previously activated with 10 mL 100% acetonitrile (ACN) followed by 10 mL 0.05% trifluoroacetic acid in water (TFA water). Following successive low ACN washes, semi-purified potato polysaccharide preparation was eluted in 10 mL 10% ACN in 0.05% TFA water. The eluent fraction was dried and reconstituted in 1 mL 0.05% TFA water for further purification via HPLC.
The reconstituted 10% ACN eluent fraction was subjected to HPLC purification utilizing a Waters Xterra RP C18 column (4.6×150 mm) and Waters 2695 separations module with a photodiode array detector. HPLC purification employed a shallow 20 minute gradient ranging from 0 to 2.5% in 0.05 TFA water at a flow rate of 0.5 mL/min. Collection and HPLC re-purification of a major 198 nm UV absorbing peak at 3.5 minutes yielded a symmetrical HPLC peak containing highly purified potato polysaccharide preparation. The purified HPLC fraction was dried and reconstituted in phosphate buffered saline (PBS) for use in biological experiments.
Following incubation of HTB-11 neuroblastoma cells with purified potato polysaccharide preparation, total RNA was isolated and purified using the RNeasy mini kit (Qiagen, Valencia, Calif.). Briefly, pelleted cells were re-suspended in 600 μL of RLT lysis buffer (Qiagen) and homogenized by passing the lysate 20 times through a 1 mL pipette tip. The samples were then processed according to the manufacturer's instructions (Qiagen, Valencia, Ca). In the final step, the RNA was eluted with 50 μL of RNase-free water by centrifugation for 1 minute at 13,000 g. The RNA was analyzed on a model 2100 bioanalyzer (Agilent, Santa Clara, Calif.) using a total RNA nanochip according to the manufacturer's protocol.
DNA microarray analyses were performed using a system provided by Agilent. Arrays included four arrays per chip (Agilent 4X44K chips). Total RNA was reverse transcribed (400 ng) using T7 primers and labeled and transcribed using Cyanine-3 dye. Each array was hybridized with at least 1.65 μg of labeled cRNA at 65° C. for 18 hours. Arrays were scanned using an Agilent array scanner. Array images were extracted with Agilent feature extraction software, and gene expression changes were calculated using Genespring version 12.6.
In vitro 4 hour administration of purified potato polysaccharide preparation to HTB-11 neuroblastoma cells engendered a statistically significant 21.4±8.0% reduction of oncogenic KRAS gene expression, as monitored by DNA microarray analyses. Additionally, coordinate reductions of interactive SHOC2 and ILK oncogene expression of 16.7±3.1% and 23.9±2.5%, respectively, were observed as depicted in Table 2. The SHOC2 gene product is a cellular scaffold protein having repetitive leucine rich repeats that putatively link KRAS/ERK/MAP kinase signaling cascades. Coordinately dysregulated oncogenic KRAS and SHOC2 expression may override normative regulation of ERK1/2 activation by the epidermal EGFR-mediated signaling in major classes of human cancers. Additionally, KRAS and SHOC2 mutations have been linked to the development of Noonan Syndrome, an autosomal dominant condition leading to hematological malignancies and specific neuroblastoma and embryonal rhabdomyosarcoma solid tumors. Oncogenic ILK is an intracellular integrin:actin-bridging protein that is functionally linked to proliferation and metastatic outgrowth of primary tumor cells. Accordingly, the case for coordinate functional recruitment of oncogenic KRAS, SHOC2, and ILK in tumor growth, survival, and metastasis exists, demonstrating that a potato polysaccharide preparation can be used to treat cancers (e.g., human cancers) alone or in combination with one or more chemotherapeutic agents.
These results also demonstrate that potato polysaccharide preparations can be used as anti-proliferative agents against cancer cells expressing a KRAS polypeptide.
To determine the effects of potato polysaccharide preparation administration on the expression of oncogenic KRAS and potentially interactive oncogenes in the compromised livers of genetically obese Zucker ZDF rats.
Twenty-two 7-week old, male Zucker Diabetic Fatty rats (ZDF, Code: 370) and twenty-two 7-8 week old, male ZDF Lean rats (Code: 371) were purchased from Charles Rivers Laboratories (Wilmington, Mass.). The study animals were allowed an acclimation period of 4 days prior to baseline blood collections, at which time two extra animals from each strain were dropped from the study based on baseline body weight. The rats were housed two per cage and maintained in the Innovive caging system (San Diego, Calif.) upon arrival at PhysioGenix, Inc. Cages were monitored daily to ensure the Innovive system maintained 80 air changes per hour and positive pressure. In accordance with the Guide for Care and Use of Laboratory Animals (Eighth Edition), rat rooms were maintained at temperatures of 66-75 degrees Fahrenheit and relative humidity between 30% and 70%. The rooms were lit by artificial light for 12 hours each day (7:00 AM-7:00 PM). Animals had free access to water and Purina 5008 rodent food (Waldschimdt's, Madison, Wis.) for the duration of the study except during fasted experiments.
Purified potato polysaccharide preparation (10 mL stock solution at 5 mg/mL concentration) was stored at 4° C. The vehicle for the study was sterile water (Catalog number 002488, Butler Schein). Each week, the stock solution was diluted 1:100 in sterile water (0.05 mg/mL) and dispensed into daily aliquots. All vehicle and drug solutions were stored at 4° C. and administered at room temperature daily by oral gavage (PO) in a volume of 1 mL/animal (0.15 mg/kg dose based on estimated body weight of 350 g).
Two types of rats were used for the study: homozygous obese ZDF/ZDF and heterozygous lean littermates. The rats within the groups were then chosen at random and divided into groups of 10. Group 1 was the homozygous ZDF/ZDF vehicle fed rats, group 2 was the homozygous ZDF/ZDF potato polysaccharide preparation fed, group 3 was the lean vehicle fed rat, and group 4 was the lean potato polysaccharide preparation fed rats. The vehicle was distilled water, and the potato polysaccharide preparation was given daily each morning via oral gavage at a dosage of 0.05 mg per animal. The dose was usually given in 1 ml of water. Rats were caged in groups and maintained in 12 hours light/12 hours dark (7 am-7 pm). The study lasted for 28 days, and all animals were euthanized by isoflurane overdose and thoracotomy following the collection of fasted blood glucose data on Day 28 of the study. Blood was collected via descending vena cava. Liver and abdominal fat were collected and weighed, and a portion of the left lateral liver lobe and abdominal fat were placed into individual histology cassettes and snap frozen in liquid nitrogen. General pathological observations were recorded.
RNA was isolated from frozen liver tissues using the Qiagen mini kit as described previously. The tissues (100 mg tissue and 1.8 mL of lysis buffer) were homogenized using a Polytron homogenizer. After the RNA was purified, 1 μg of total RNA was reverse transcribed using Superscript III reverse transcriptase and random primers (Invitrogen). Real-time PCR was performed with KRAS, SHOC2, ILK, and HSP9a detector sets (Applied Biosystem). SRSF4 was used as a reference gene. Using 1 μL of cDNA per reaction, all samples were analyzed in triplicate. The Real-time PCR master mix included 25 μL 2× universal master mix, 2.5 μL 20× detector set (with the primer and probe), and 21.5 μL of water. PCR was performed in an Applied Biosystems 7500 sequence detection system. The thermocycler conditions included denaturation at 95° C. for 15 seconds and annealing/extension at 60° C. for 60 seconds. Forty cycles of PCR were preceded by 95° C. for 10 minutes. The relative quantities of genes were found using the formula 2-ΔΔCt using the Applied Biosystems 7500 software.
In vivo administration of purified potato polysaccharide preparation to ZDF rats (n=5) vs. vehicle control ZDF rats engendered a statistically significant 63.0±4.0% (p=0.01) reduction of oncogenic KRAS gene expression in compromised live tissues, as monitored by Real-time PCR analyses (
Furthermore, in vivo administration of purified potato polysaccharide preparation to heterozygous lean ZDF rat litter mates vs. vehicle control rats resulted in statistically significant decreases of 11.2±2.8% and 26.2±2.5% (p=0.04) in SHOC2 (n=6) and HSPA9 (n=6) oncogene expression, respectively, in liver tissues. These results demonstrate that a potato polysaccharide preparation provided herein can be used to reduce the risk of developing cancer.
It is to be understood that while the invention has been described in conjunction with the detailed description thereof, the foregoing description is intended to illustrate and not limit the scope of the invention, which is defined by the scope of the appended claims. Other aspects, advantages, and modifications are within the scope of the following claims.
| Filing Document | Filing Date | Country | Kind |
|---|---|---|---|
| PCT/US16/24283 | 3/25/2016 | WO | 00 |
| Number | Date | Country | |
|---|---|---|---|
| 62139163 | Mar 2015 | US |
