Patent Grant
12117378
Samples may be processed for various purposes, such as identification of a type of moiety within the sample. The sample may be a biological sample. The biological samples may be processed for various purposes, such as detection of a disease (e.g., cancer) or identification of a particular species. There are various approaches for processing samples, such as polymerase chain reaction (PCR) and sequencing.
Biological samples may be processed within various reaction environments, such as partitions. Partitions may be wells or droplets. Droplets or wells may be employed to process biological samples in a manner that enables the biological samples to be partitioned and processed separately. For example, such droplets may be fluidically isolated from other droplets, enabling accurate control of respective environments in the droplets.
Partitions and/or biological samples in partitions may be subjected to various processes, such as chemical processes or physical processes. Partitions and/or samples in partitions may be subjected to heating or cooling, or chemical reactions, such as to yield species that may be qualitatively or quantitatively processed.
The present disclosure provides methods for use in various sample processing and analysis applications. The methods provided herein may increase a concentration of a first set of molecules within a sample relative to a second set of molecules within the same sample, thereby enriching the first set of molecules within the sample. Such methods may be useful, for example, in controlled analysis and processing of analytes such as biological particles, nucleic acids, and proteins.
In an aspect, the present disclosure provides a method of processing a cell, comprising: (a) subjecting the cell to conditions sufficient to: (i) change a cross-section of the cell from a first cross-section to a second cross-section, which second cross-section is less than the first cross-section, and (ii) form crosslinks within the cell having the second cross-section; and (b) providing the cell having the second cross-section in an aqueous fluid.
In some embodiments, the crosslinks are formed upon cross-linking one or more cross-linkable molecules within the cell. In some embodiments, the one or more cross-linkable molecules are one or more polymers.
In some embodiments, the crosslinks are formed upon polymerizing a plurality of monomers within the cell.
In some embodiments, the cross-section of the cell is changed from the first cross-section to the second cross-section concurrently with formation of the crosslinks within the cell.
In some embodiments, the crosslinks are formed subsequent to changing the cross-section from the first cross-section to the second cross-section.
In some embodiments, the second cross-section is substantially maintained in the aqueous fluid.
In some embodiments, the aqueous fluid is in a droplet as part of an emulsion.
In some embodiments, the volume of the droplet is less than 10,000 pL. In some embodiments, the volume of the droplet is less than 1,000 pL. In some embodiments, the volume of the droplet is less than 500 pL. In some embodiments, the volume of the droplet is less than 100 pL. In some embodiments, the volume of the droplet is less than 50 pL. In some embodiments, the volume of the droplet is less than 10 pL.
In some embodiments, the aqueous fluid is in a well, such as a well of a plurality of wells.
In some embodiments, (a) comprises bringing the cell in contact with a first chemical species and a second chemical species, wherein (i) comprises using the first chemical species to change the cross-section from the first-cross-section to the second cross-section, and wherein (ii) comprises using the second chemical species to form the crosslinks within the cell.
In some embodiments, the first or second chemical species is selected from the group consisting of disuccinimidyl suberate (DSS), dimethylsuberimidate (DMS), formalin, and dimethyladipimidate (DMA), dithio-bis(-succinimidyl propionate) (DSP), disuccinimidyl tartrate (DST), and ethylene glycol bis(succinimidyl succinate) (EGS). In some embodiments, the first and/or the second chemical species is selected from the group consisting of an organic solvent or a cross-linking agent. In some embodiments, the first chemical species is an organic solvent and the second chemical species is a cross-linking agent. In some embodiments, the organic solvent is acetone. In some embodiments, the organic solvent is an alcohol. In some embodiments, the alcohol is methanol or ethanol. In some embodiments, the cross-linking agent is photocleavable crosslinker. In some embodiments, the cross-linking agent is an aldehyde. In some embodiments, the cross-linking agent is formaldehyde or glutaraldehyde.
In some embodiments, the method further comprises providing the cell in an aqueous reaction mixture, wherein the cell comprises a target molecule, and performing one or more reactions using the target molecule. In some embodiments, the method further comprises co-partitioning the cell in a partition and performing the one or more reactions in the partition. In some embodiments, the partition is a droplet. In some embodiments, the partition is a well. In some embodiments, the target molecule is a nucleic acid molecule and wherein the partition further comprises a plurality of nucleic acid barcode molecules, wherein each nucleic acid barcode molecule of the plurality of nucleic acid barcode molecules comprises a sequence comprising a common barcode sequence. In some embodiments, the plurality of nucleic acid barcode molecules are attached to a bead. In some embodiments, sequences of the plurality of nucleic acid barcode molecules are releasably attached to the bead. In some embodiments, the method further comprises releasing the sequences of the plurality of nucleic acid barcode molecules from the bead within the partition. In some embodiments, the bead is a gel bead. In some embodiments, the gel bead is degradable upon application of a stimulus. In some embodiments, the stimulus is a chemical stimulus. In some embodiments, the stimulus is a reducing agent. In some embodiments, the partition further comprises the chemical stimulus. In some embodiments, the one or more reactions comprise barcoding the target molecule using a nucleic acid barcode molecule of the plurality of nucleic acid barcode molecules.
In some embodiments, the cross-section is a diameter of the cell.
In some embodiments, the cross-section is a volume of the cell.
In some embodiments, the second cross-section of the cell is reduced by at least 5% compared to the first cross-section. In some embodiments, the second cross-section of the cell is reduced by at least 10% compared to the first cross-section. In some embodiments, the second cross-section of the cell is reduced by at least 15% compared to the first cross-section. In some embodiments, the second cross-section of the cell is reduced by at least 25% compared to the first cross-section. In some embodiments, the second cross-section of the cell is reduced by at least 50% compared to the first cross-section.
In some embodiments, the change from the first cross-section of the cell to the second cross-section is irreversible.
In some embodiments, the change from the first cross-section of the cell to the second cross-section is reversible. In some embodiments, the change from the first cross-section of the cell to the second cross-section is reversible upon application of a stimulus. In some embodiments, the stimulus is selected from the group consisting of a thermal stimulus, a photo stimulus, and a chemical stimulus. In some embodiments, the stimulus comprises a change in pH. In some embodiments, the stimulus comprises a reducing agent. In some embodiments, the reducing agent comprises dithiothreitol. In some embodiments, the method further comprises applying the stimulus, wherein application of the stimulus reverses the change from the first cross-section to the second cross-section by at least 75%.
In an aspect, the present disclosure provides a method of processing a bead, comprising: subjecting the bead to conditions sufficient to change a cross-section of the bead from a first cross-section to a second cross-section, which second cross-section is less than the first cross-section.
In some embodiments, the bead is a cell bead. In some embodiments, the bead is a gel bead.
In some embodiments, the subjecting the bead to the conditions comprises bringing the bead in contact with a chemical species, wherein the chemical species subjects the cross-section to change from the first-cross-section to the second cross-section.
In some embodiments, the chemical species is an organic solvent. In some embodiments, the organic solvent is acetone. In some embodiments, the organic solvent is an alcohol.
In some embodiments, the subjecting the bead to the conditions comprises changing the temperature. In some embodiments, the bead comprises a polymer that is responsive to temperature. In some embodiments, the polymer is poly (N-isopropylacrylamide).
In some embodiments, the method further comprises partitioning the bead having the second cross-section into a partition. In some embodiments, the method further comprises co-partitioning into the partition the bead having the second cross-section and an additional bead. In some embodiments, the method further comprises subjecting the additional bead to additional conditions sufficient to change a cross-section of the additional bead from a third cross-section to a fourth cross-section, which fourth cross-section is less than the third cross-section.
In some embodiments, the additional bead is a cell bead. In some embodiments, the additional bead is a gel bead.
In some embodiments, the additional conditions are the same as the conditions. In some embodiments, the additional conditions and the conditions are different. In some embodiments, the additional conditions are applied prior to the co-partitioning. In some embodiments, the additional conditions are applied during the co-partitioning. In some embodiments, the additional conditions are applied subsequent to the co-partitioning.
In some embodiments, the conditions are applied prior to the partitioning. In some embodiments, the conditions are applied during the partitioning. In some embodiments, the conditions are applied subsequent to the partitioning.
In some embodiments, the method further comprises co-partitioning into the partition the bead having the second cross-section and a cell.
In some embodiments, the cell is subjected to additional conditions sufficient to: (i) change a a cross-section of said cell from a first cross-section to a second cross-section, which second cross-section is less than said first cross-section, and (ii) form crosslinks within said cell having said second cross-section. In some embodiments, the partition is a droplet (e.g., a droplet as part of an emulsion). In some embodiments, the partition is in a well (e.g., as part of a plurality of wells).
Additional aspects and advantages of the present disclosure will become readily apparent to those skilled in this art from the following detailed description, wherein only illustrative embodiments of the present disclosure are shown and described. As will be realized, the present disclosure is capable of other and different embodiments, and its several details are capable of modifications in various obvious respects, all without departing from the disclosure. Accordingly, the drawings and description are to be regarded as illustrative in nature, and not as restrictive.
All publications, patents, and patent applications mentioned in this specification are herein incorporated by reference to the same extent as if each individual publication, patent, or patent application was specifically and individually indicated to be incorporated by reference. To the extent publications and patents or patent applications incorporated by reference contradict the disclosure contained in the specification, the specification is intended to supersede and/or take precedence over any such contradictory material.
The novel features of the invention are set forth with particularity in the appended claims. A better understanding of the features and advantages of the present invention will be obtained by reference to the following detailed description that sets forth illustrative embodiments, in which the principles of the invention are utilized, and the accompanying drawings (also “Figure” and “FIG.” herein), of which:
While various embodiments of the invention have been shown and described herein, it will be obvious to those skilled in the art that such embodiments are provided by way of example only. Numerous variations, changes, and substitutions may occur to those skilled in the art without departing from the invention. It should be understood that various alternatives to the embodiments of the invention described herein may be employed.
Where values are described as ranges, it will be understood that such disclosure includes the disclosure of all possible sub-ranges within such ranges, as well as specific numerical values that fall within such ranges irrespective of whether a specific numerical value or specific sub-range is expressly stated.
The term “barcode,” as used herein, generally refers to a label, or identifier, that conveys or is capable of conveying information about an analyte. A barcode can be part of an analyte. A barcode can be independent of an analyte. A barcode can be a tag attached to an analyte (e.g., nucleic acid molecule) or a combination of the tag in addition to an endogenous characteristic of the analyte (e.g., size of the analyte or end sequence(s)). A barcode may be unique. Barcodes can have a variety of different formats. For example, barcodes can include: polynucleotide barcodes; random nucleic acid and/or amino acid sequences; and synthetic nucleic acid and/or amino acid sequences. A barcode can be attached to an analyte in a reversible or irreversible manner. A barcode can be added to, for example, a fragment of a deoxyribonucleic acid (DNA) or ribonucleic acid (RNA) sample before, during, and/or after sequencing of the sample. Barcodes can allow for identification and/or quantification of individual sequencing-reads.
The term “real time,” as used herein, can refer to a response time of less than about 1 second, a tenth of a second, a hundredth of a second, a millisecond, or less. The response time may be greater than 1 second. In some instances, real time can refer to simultaneous or substantially simultaneous processing, detection or identification.
The term “subject,” as used herein, generally refers to an animal, such as a mammal (e.g., human) or avian (e.g., bird), or other organism, such as a plant. For example, the subject can be a vertebrate, a mammal, a rodent (e.g., a mouse), a primate, a simian or a human. Animals may include, but are not limited to, farm animals, sport animals, and pets. A subject can be a healthy or asymptomatic individual, an individual that has or is suspected of having a disease (e.g., cancer) or a pre-disposition to the disease, and/or an individual that is in need of therapy or suspected of needing therapy. A subject can be a patient. A subject can be a microorganism or microbe (e.g., bacteria, fungi, archaea, viruses).
The term “genome,” as used herein, generally refers to genomic information from a subject, which may be, for example, at least a portion or an entirety of a subject's hereditary information. A genome can be encoded either in DNA or in RNA. A genome can comprise coding regions (e.g., that code for proteins) as well as non-coding regions. A genome can include the sequence of all chromosomes together in an organism. For example, the human genome ordinarily has a total of 46 chromosomes. The sequence of all of these together may constitute a human genome.
The terms “adaptor(s)”, “adapter(s)” and “tag(s)” may be used synonymously. An adaptor or tag can be coupled to a polynucleotide sequence to be “tagged” by any approach, including ligation, hybridization, or other approaches.
The term “sequencing,” as used herein, generally refers to methods and technologies for determining the sequence of nucleotide bases in one or more polynucleotides. The polynucleotides can be, for example, nucleic acid molecules such as deoxyribonucleic acid (DNA) or ribonucleic acid (RNA), including variants or derivatives thereof (e.g., single stranded DNA). Sequencing can be performed by various systems currently available, such as, without limitation, a sequencing system by Illumina®, Pacific Biosciences (PacBio®), Oxford Nanopore®, or Life Technologies (Ion Torrent®). Alternatively or in addition, sequencing may be performed using an extension reaction, nucleic acid amplification, polymerase chain reaction (PCR) (e.g., digital PCR, quantitative PCR, or real time PCR), or isothermal amplification. Such systems may provide a plurality of raw genetic data corresponding to the genetic information of a subject (e.g., human), as generated by the systems from a sample provided by the subject. In some examples, such systems provide sequencing reads (also “reads” herein). A read may include a string of nucleic acid bases corresponding to a sequence of a nucleic acid molecule that has been sequenced. In some situations, systems and methods provided herein may be used with proteomic information.
The term “bead,” as used herein, generally refers to a particle. The bead may be a solid or semi-solid particle. The bead may be a gel bead. The gel bead may include a polymer matrix (e.g., matrix formed by polymerization or cross-linking). The polymer matrix may include one or more polymers (e.g., polymers having different functional groups or repeat units). Polymers in the polymer matrix may be randomly arranged, such as in random copolymers, and/or have ordered structures, such as in block copolymers. Cross-linking can be via covalent, ionic, or inductive, interactions, or physical entanglement. The bead may be a macromolecule. The bead may be formed of nucleic acid molecules bound together. The bead may be formed via covalent or non-covalent assembly of molecules (e.g., macromolecules), such as monomers or polymers. Such polymers or monomers may be natural or synthetic. Such polymers or monomers may be or include, for example, nucleic acid molecules (e.g., DNA or RNA). The bead may be formed of a polymeric material. The bead may be magnetic or non-magnetic. The bead may be rigid. The bead may be flexible and/or compressible. The bead may be disruptable or dissolvable. The bead may be a solid particle (e.g., a metal-based particle including but not limited to iron oxide, gold or silver) covered with a coating comprising one or more polymers. Such coating may be disruptable or dissolvable.
The term “sample,” as used herein, generally refers to a biological sample of a subject. The biological sample may comprise any number of macromolecules, for example, macromolecules of biological particles such as cellular macromolecules. The sample may be a cell sample. The sample may be a cell line or cell culture sample. The sample can include one or more cells. The sample can include one or more microbes. The biological sample may be a nucleic acid sample or protein sample. The biological sample may also be a carbohydrate sample or a lipid sample. The biological sample may be derived from another sample. The sample may be a tissue sample, such as a biopsy, core biopsy, needle aspirate, or fine needle aspirate. The sample may be a fluid sample, such as a blood sample, urine sample, or saliva sample. The sample may be a skin sample. The sample may be a cheek swab. The sample may be a plasma or serum sample. The sample may be a cell-free or cell free sample. A cell-free sample may include extracellular polynucleotides. Extracellular polynucleotides may be isolated from a bodily sample that may be selected from the group consisting of blood, plasma, serum, urine, saliva, mucosal excretions, sputum, stool and tears.
The term “biological particle,” as used herein, generally refers to a discrete biological system derived from a biological sample. The biological particle may be a macromolecule. The biological particle may be a small molecule. The biological particle may be a virus. The biological particle may be a cell or derivative of a cell. The biological particle may be an organelle. The biological particle may be a rare cell from a population of cells. The biological particle may be any type of cell, including without limitation prokaryotic cells, eukaryotic cells, bacterial, fungal, plant, mammalian, or other animal cell type, mycoplasmas, normal tissue cells, tumor cells, or any other cell type, whether derived from single cell or multicellular organisms. The biological particle may be a constituent of a cell. The biological particle may be or may include DNA, RNA, organelles, proteins, or any combination thereof. The biological particle may be or may include a matrix (e.g., a gel or polymer matrix) comprising a cell or one or more constituents from a cell (e.g., cell bead), such as DNA, RNA, organelles, proteins, or any combination thereof, from the cell. The biological particle may be obtained from a tissue of a subject. The biological particle may be a hardened cell. Such hardened cell may or may not include a cell wall or cell membrane. The biological particle may include one or more constituents of a cell, but may not include other constituents of the cell. An example of such constituents is a nucleus or an organelle. A cell may be a live cell. The live cell may be capable of being cultured, for example, being cultured when enclosed in a gel or polymer matrix, or cultured when comprising a gel or polymer matrix.
The term “macromolecular constituent,” as used herein, generally refers to a macromolecule contained within or from a biological particle. The macromolecular constituent may comprise a nucleic acid. In some cases, the biological particle may be a macromolecule. The macromolecular constituent may comprise DNA. The macromolecular constituent may comprise RNA. The RNA may be coding or non-coding. The RNA may be messenger RNA (mRNA), ribosomal RNA (rRNA) or transfer RNA (tRNA), for example. The RNA may be a transcript. The RNA may be small RNA that are less than 200 nucleic acid bases in length, or large RNA that are greater than 200 nucleic acid bases in length. Small RNAs may include 5.8S ribosomal RNA (rRNA), 5S rRNA, transfer RNA (tRNA), microRNA (miRNA), small interfering RNA (siRNA), small nucleolar RNA (snoRNAs), Piwi-interacting RNA (piRNA), tRNA-derived small RNA (tsRNA) and small rDNA-derived RNA (srRNA). The RNA may be double-stranded RNA or single-stranded RNA. The RNA may be circular RNA. The macromolecular constituent may comprise a protein. The macromolecular constituent may comprise a peptide. The macromolecular constituent may comprise a polypeptide.
The term “molecular tag,” as used herein, generally refers to a molecule capable of binding to a macromolecular constituent. The molecular tag may bind to the macromolecular constituent with high affinity. The molecular tag may bind to the macromolecular constituent with high specificity. The molecular tag may comprise a nucleotide sequence. The molecular tag may comprise a nucleic acid sequence. The nucleic acid sequence may be at least a portion or an entirety of the molecular tag. The molecular tag may be a nucleic acid molecule or may be part of a nucleic acid molecule. The molecular tag may be an oligonucleotide or a polypeptide. The molecular tag may comprise a DNA aptamer. The molecular tag may be or comprise a primer. The molecular tag may be, or comprise, a protein. The molecular tag may comprise a polypeptide. The molecular tag may be a barcode.
The term “partition,” as used herein, generally, refers to a space or volume that may be suitable to contain one or more species or conduct one or more reactions. A partition may be a physical compartment, such as a droplet or well. The partition may isolate space or volume from another space or volume. The droplet may be a first phase (e.g., aqueous phase) in a second phase (e.g., oil) immiscible with the first phase. The droplet may be a first phase in a second phase that does not phase separate from the first phase, such as, for example, a capsule or liposome in an aqueous phase. A partition may comprise one or more other (inner) partitions. In some cases, a partition may be a virtual compartment that can be defined and identified by an index (e.g., indexed libraries) across multiple and/or remote physical compartments. For example, a physical compartment may comprise a plurality of virtual compartments.
Provided herein are methods that may be used for various sample processing and analysis applications. The methods provided herein may comprise processing of a cell and/or a particle (e.g., bead, such as a gel bead). The methods provided herein may alter one or more characteristics of the cell and/or particle, such as a size (e.g., diameter or cross-section) and/or volume of the cell and/or particle. Methods comprising cell processing may provide cell beads comprising a plurality of crosslinks (e.g., a plurality of crosslinks between one or more polymer molecules). A method of processing a cell may comprise subjecting the cell to conditions sufficient to change a characteristic (e.g., a cross-section) of the cell. This process may involve the use of a chemical species and may in some case result in polymerization within and/or around the cell. Alternatively or in addition, the cell may be contacted with an additional chemical species, which additional chemical species may further alter the characteristic of the cell or alter an additional characteristic of the cell. The additional chemical species may be used to promote the formation of crosslinks within the cell (e.g., to promote the formation of a polymer network within the cell). A cell may be processed within a bulk solution. Alternatively, a cell may be processed within a partition such as a droplet or well. The droplet, well, or bulk solution may comprise an aqueous fluid. The cell and/or component of the cell (e.g., nucleic acid molecules included therein) may undergo further processing, such as within a partition (e.g., droplet or well). Particles may be subjected to similar processing. For example, a particle (e.g., bead) may be subjected to conditions sufficient to change a characteristic (e.g., cross-section) of the particle. One or more chemical species may be used to change the characteristic of the particle. In some cases, a first chemical species may be used to change the characteristic of the particle and a second chemical species may be used to further alter the characteristic or to change another characteristic of the particle. The present disclosure also provides kits and compositions comprising cells and/or particles processed according to the methods provided herein and kits for processing cells and/or particles according to the methods provided herein.
Methods of Processing Cells and Beads
In an aspect, the present disclosure provides a method for processing a cell. The method may comprise subjecting a cell to conditions sufficient to change one or more characteristics of the cell and subsequently providing the cell for further processing. In some cases, one or more physical parameters or dimensions and/or one or more other characteristics of the cell may be changed. For example, a cross-section of the cell may be changed from a first cross-section to a second cross-section. The first cross-section may be smaller or larger than the second cross-section. Alternatively or in addition, one or more other characteristics of the cell may be changed. For example, the fluidity, density, rigidity, porosity, or other characteristic of the cell or one or more components thereof may be changed. In an example, a polymer and/or gel matrix may be formed within the cell. In another example, crosslinks (e.g., crosslinked proteins or polymers) may be formed within the cell. The same or different conditions may be used to change or affect different characteristics of the cell at the same or different times. In some cases, a first condition or set of conditions may be used to change a first characteristic or set of characteristics of the cell (e.g., a cross-section) and a second condition or set of conditions may be used to change a second characteristic or set of characteristics of the cell. The first condition or set of conditions may be applied at the same or a different time as the second condition or set of conditions. For example, a first characteristic or set of characteristics may be changed under a first condition or set of conditions, after which a second characteristic or set of characteristics may be changed under a second condition or set of conditions. The first condition or set of conditions may comprise a first chemical species and the second condition or set of conditions may comprise a second chemical species, where the first chemical species and the second chemical species may be the same or different. In some cases, the first chemical species may be an organic solvent and the second chemical species may be a cross-linking agent.
The cell may be derived from any suitable source. For example, the cell may derive from a biological sample or an environmental sample. In some cases, the cell may derive from a bodily sample (e.g., as described herein). For example, the cell may derive from a sample comprising blood, plasma, serum, urine, saliva, mucosal excretions, sputum, stool, tears and/or another bodily fluid. A sample comprising the cell may be subjected to various processing including, for example, filtration, centrifugation, extraction, washing, and elution. In some cases, the cell may be isolated from a biological sample or an environmental sample. For example, the cell may be isolated from a blood sample.
The cell may be of any type (e.g., as described herein). For example, the cell may be a mammalian, fungal, plant, bacterial, or other cell type. In some cases, the cell is a mammalian cell, such as a human cell. The cell may be, for example, a stem cell, liver cell, nerve cell, bone cell, blood cell, reproductive cell, skin cell, skeletal muscle cell, cardiac muscle cell, smooth muscle cell, hair cell, hormone-secreting cell, or glandular cell. The cell may be, for example, an erythrocyte (e.g., red blood cell), a megakaryocyte (e.g., platelet precursor), a monocyte (e.g., white blood cell), a leukocyte, a B cell, a T cell (such as a helper, suppressor, cytotoxic, or natural killer T cell), an osteoclast, a dendritic cell, a connective tissue macrophage, an epidermal Langerhans cell, a microglial cell, a granulocyte, a hybridoma cell, a mast cell, a natural killer cell, a reticulocyte, a hematopoietic stem cell, a myoepithelial cell, a myeloid-derived suppressor cell, a platelet, a thymocyte, a satellite cell, an epithelial cell, an endothelial cell, an epididymal cell, a kidney cell, a liver cell, an adipocyte, a lipocyte, or a neuron cell. In some cases, the cell may be associated with a cancer, tumor, or neoplasm. In some cases, the cell may be associated with a fetus. In some cases, the cell may be a Jurkat cell.
The cell may have any feature or dimension. For example, the cell may have a first dimension, a second dimension, and a third dimension, where the first, second, and third dimensions are approximately the same. In other cases, the first and second dimensions may be approximately the same, and the third dimension may be different, or the first, second, and third dimensions may all be different. In some cases, the cell may comprise a dimension (e.g., a diameter) of at least about 1 μm. For example, the cell may comprise a dimension of at least about 1 micrometer (μm), 2 μm, 3 μm, 4 μm, 5 μm, 6 μm, 7 μm, 8 μm, 9 μm, 10 μm, 11 μm, 12 μm, 13 μm, 14 μm, 15 μm, 16 μm, 17 μm, 18 μm, 19 μm, 20 μm, 25 μm, 30 μm, 35 μm, 40 μm, 45 μm, 50 μm, 55 μm, 60 μm, 65 μm, 70 μm, 75 μm, 80 μm, 85 μm, 90 μm, 100 μm, 120 μm, 140 μm, 160 μm, 180 μm, 200 μm, 250 μm, 300 μm, 350 μm, 400 μm, 450 μm, 500 μm, 550 μm, 600 μm, 650 μm, 700 μm, 750 μm, 800 μm, 850 μm, 900 μm, 950 μm, 1 millimeter (mm), or greater. In some cases, the cell may comprise a dimension of between about 1 μm and 500 μm, such as between about 1 μm and 100 μm, between about 100 μm and 200 μm, between about 200 μm and 300 μm, between about 300 μm and 400 μm, or between about 400 μm and 500 μm. For example, the cell may comprise a dimension of between about 1 μm and 100 μm. Any or all dimensions of the cell may be variable. For example, the dimensions of a substantially fluid cell may vary over a rapid timescale. Dimensions of a more rigid cell may be fixed or may vary with lesser amplitude. Accordingly, the dimensions provided herein may represent averages rather than fixed values. The volume of the cell may be at least about 1 μm3. In some cases, the volume of the cell may be at least about 10 μm3. For example, the volume of the cell may be at least 1 μm3, 2 μm3, 3 μm3, 4 μm3, 5 μm3, 6 μm3, 7 μm3, 8 μm3, 9 μm3, 10 μm3, 12 μm3, 14 μm3, 16 μm3, 18 μm3, 20 μm3, 25 μm3, 30 μm3, 35 μm3, 40 μm3, 45 μm3, 50 μm3, 55 μm3, 60 μm3, 65 μm3, 70 μm3, 75 μm3, 80 μm3, 85 μm3, 90 μm3, 95 μm3, 100 μm3, 125 μm3, 150 μm3, 175 μm3, 200 μm3, 250 μm3, 300 μm3, 350 μm3, 400 μm3, 450 μm3, m3, 500 μm3, 550 μm3, 600 μm3, 650 μm3, 700 μm3, 750 μm3, 800 μm3, 850 μm3, 900 μm3, 950 μm3, 1000 μm3, 1200 μm3, 1400 μm3, 1600 μm3, 1800 μm3, 2000 μm3, 2200 μm3, 2400 μm3, 2600 μm3, 2800 μm3, 3000 μm3, or greater. In some cases, the cell may comprise a volume of between about 1 μm3 and 100 μm3, such as between about 1 μm3 and 10 μm3, between about 10 μm3 and 50 μm3, or between about 50 μm3 and 100 μm3. In some cases, the cell may comprise a volume of between about 100 μm3 and 1000 μm3, such as between about 100 μm3 and 500 μm3 or between about 500 μm3 and 1000 μm3. In some cases, the cell may comprise a volume between about 1000 μm3 and 3000 μm3, such as between about 1000 μm3 and 2000 μm3 or between about 2000 μm3 and 3000 μm3. In some cases, the cell may comprise a volume between about 1 μm3 and 3000 μm3, such as between about 1 μm3 and 2000 μm3, between about 1 μm3 and 1000 μm3, between about 1 μm3 and 500 μm3, or between about 1 μm3 and 250 μm3.
The cell may comprise one or more cross-sections that may be the same or different. In some cases, the cell may have a first cross-section that is different from a second cross-section. The cell may have a first cross-section that is at least about 1 μm. For example, the cell may comprise a cross-section (e.g., a first cross-section) of at least about 1 micrometer (μm), 2 μm, 3 μm, 4 μm, 5 μm, 6 μm, 7 μm, 8 μm, 9 μm, 10 μm, 11 μm, 12 μm, 13 μm, 14 μm, 15 μm, 16 μm, 17 μm, 18 μm, 19 μm, 20 μm, 25 μm, 30 μm, 35 μm, 40 μm, 45 μm, 50 μm, 55 μm, 60 μm, 65 μm, 70 μm, 75 μm, 80 μm, 85 μm, 90 μm, 100 μm, 120 μm, 140 μm, 160 μm, 180 μm, 200 μm, 250 μm, 300 μm, 350 μm, 400 μm, 450 μm, 500 μm, 550 μm, 600 μm, 650 μm, 700 μm, 750 μm, 800 μm, 850 μm, 900 μm, 950 μm, 1 millimeter (mm), or greater. In some cases, the cell may comprise a cross-section (e.g., a first cross-section) of between about 1 μm and 500 μm, such as between about 1 μm and 100 μm, between about 100 μm and 200 μm, between about 200 μm and 300 μm, between about 300 μm and 400 μm, or between about 400 μm and 500 μm. For example, the cell may comprise a cross-section (e.g., a first cross-section) of between about 1 μm and 100 μm. In some cases, the cell may have a second cross-section that is at least about 1 μm. For example, the cell may comprise a second cross-section of at least about 1 micrometer (μm), 2 μm, 3 μm, 4 μm, 5 μm, 6 μm, 7 μm, 8 μm, 9 μm, 10 μm, 11 μm, 12 μm, 13 μm, 14 μm, 15 μm, 16 μm, 17 μm, 18 μm, 19 μm, 20 μm, 25 μm, 30 μm, 35 μm, 40 μm, 45 μm, 50 μm, 55 μm, 60 μm, 65 μm, 70 μm, 75 μm, 80 μm, 85 μm, 90 μm, 100 μm, 120 μm, 140 μm, 160 μm, 180 μm, 200 μm, 250 μm, 300 μm, 350 μm, 400 μm, 450 μm, 500 μm, 550 μm, 600 μm, 650 μm, 700 μm, 750 μm, 800 μm, 850 μm, 900 μm, 950 μm, 1 millimeter (mm), or greater. In some cases, the cell may comprise a second cross-section of between about 1 μm and 500 μm, such as between about 1 μm and 100 μm, between about 100 μm and 200 μm, between about 200 μm and 300 μm, between about 300 μm and 400 μm, or between about 400 μm and 500 μm. For example, the cell may comprise a second cross-section of between about 1 μm and 100 μm.
A cross section (e.g., a first cross-section) may correspond to a diameter of the cell. In some cases, the cell may be approximately spherical. In such cases, the first cross-section may correspond to the diameter of the cell. In other cases, the cell may be approximately cylindrical. In such cases, the first cross-section may correspond to a diameter, length, or width along the approximately cylindrical cell. In some cases, the cell may comprise a surface. A cell surface may comprise one or more features. For example, a cell may comprise a dendritic receiver, flagella, roughed border, or other feature.
A characteristic or set of characteristics of the cell may be changed by one or more conditions. A condition suitable for changing a characteristic or set of characteristics of the cell may be, for example, a temperature, a pH, an ion or salt concentration, a pressure, or another condition. For example, the cell may be exposed to a chemical species that may bring about a change in one or more characteristics of the cell. In some cases, a stimulus may be used to change one or more characteristics of the cell. For example, upon application of the stimulus, one or more characteristics of the cell may be changed. The stimulus may be, for example, a thermal stimulus, a photo stimulus, a chemical stimulus, or another stimulus. In some cases, conditions sufficient to change the one or more characteristics of the cell may comprise one or more different conditions, such as a temperature and a pressure, a pH and a salt concentration, a chemical species and a temperature, or any other combination of conditions. A temperature sufficient for changing one or more characteristics of the cell may be, for example, at least about 0 degrees Celsius (° C.), 1° C., 2° C., 3° C., 4° C., 5° C., 10° C., or higher. For example, the temperature may be about 4° C. In other cases, a temperature sufficient for changing one or more characteristics of the cell may be, for example, at least about 25° C., 30° C., 35° C., 37° C., 40° C., 45° C., 50° C., or higher. For example, the temperature may be about 37° C. A pH sufficient for changing one or more characteristics of the cell may be, for example, between about 5 and 8, such as between about 6 and 7.
In some cases, a chemical species or a chemical stimulus may be used to change one or more characteristics of the cell. For example, a chemical species or a chemical stimulus may be used to change a dimension of a cell (e.g., a cross-section, diameter, or volume). In some cases, a chemical species or a chemical stimulus may be used to change a dimension of a cell (e.g., a cross-sectional diameter) from a first dimension to a second dimension (e.g., a second cross-sectional dimeter), where the second dimension is reduced compared to the first dimension. The chemical species may comprise an organic solvent, such as an alcohol, ketone, or aldehyde. For example, the chemical species may comprise acetone, methanol, ethanol, formaldehyde, or glutaraldehyde. The chemical species may comprise a cross-linking agent. For example, the chemical species may be selected from the group consisting of disuccinimidyl suberate (DSS), dimethylsuberimidate (DMS), formalin, and dimethyladipimidate (DMA), dithio-bis(-succinimidyl propionate) (DSP), disuccinimidyl tartrate (DST), and ethylene glycol bis(succinimidyl succinate) (EGS). In some cases, a cross-linking agent may be a photocleavable cross-linking agent. In some cases, a chemical stimulus may be used to change one or more characteristics of the cell (e.g., a dimension of a cell), where the chemical stimulus comprises one or more chemical species. For example, the chemical stimulus may comprise a first chemical species and a second chemical species, where the first chemical species is an organic solvent and the second chemical species is a cross-linking agent. In some cases, a chemical stimulus may comprise a chemical species that is a fixation agent that is capable of fixing or preserving a cell. For example, an organic solvent such as an alcohol (e.g., ethanol or methanol), ketone (e.g., acetone), or aldehyde (e.g., formaldehyde or glutaraldehyde) may act as a fixation agent. Alternatively, or in addition, a cross-linking agent may act as a fixation agent. In some cases, a fixation agent may be selected from the group consisting of disuccinimidyl suberate (DSS), dimethylsuberimidate (DMS), formalin, and dimethyladipimidate (DMA), dithio-bis(-succinimidyl propionate) (DSP), disuccinimidyl tartrate (DST), and ethylene glycol bis(succinimidyl succinate) (EGS). In some cases, a first chemical species and/or fixation agent may be provided to or brought into contact with the cell to bring about a change in a first characteristic or set of characteristics of the cell, and a second chemical species and/or fixation agent may be provided to or brought into contact with the cell to bring about a change in a second characteristic or set of characteristics of the cell. For example, a first chemical species and/or fixation agent may be provided to or brought into contact with the cell to bring about a change in a dimension of a cell (e.g., a reduction in cross-sectional diameter), and a second chemical species and/or fixation agent may be provided to or brought into contact with the cell to bring about a change in a second characteristic or set of characteristics of the cell (e.g., forming crosslinks within and/or surrounding the cell). The first and second chemical species and/or fixation agents may be provided to or brought into contact with the cell at the same or different times.
Fixation may comprise dehydration of the cell. In some cases, fixation may affect one or more parameters or characteristics of the cell. For example, fixation may result in shrinkage or size reduction of the cell. Providing a fixation agent to the cell may result in a change in a dimension of the cell. For example, providing a fixation agent to the cell may result in a change in the volume or diameter of the cell. Providing a fixation agent to the cell may result in a change in a cross-section of the cell (e.g., a cross-sectional diameter). For example, a first cross-section of the cell prior to fixation may be different (e.g., larger) than a second cross-section of the cell following fixation. In an example, an approximately spherical cell may comprise a first cross section (e.g., a cross-sectional diameter) prior to fixation that is reduced in size to a second cross-section following fixation. Providing a fixation agent to the cell may result in a second cross-section that is reduced by at least about 5% compared to the first cross-section. In some cases, the second cross-section may be reduced by at least 6%, 8%, 10%, 15%, 25%, 30%, 35%, 40%, 45%, 50%, or more relative to the first cross-section. For example, the second cross-section may be reduced by at least about 10%, 15%, 25%, or 50% relative to the first cross-section. Fixation may also affect other features of the cell. For example, fixation may result in a change in the porosity of a membrane or wall of a cell; reorganization of components of the cell; a change in cell fluidity or rigidity; or other changes. In an example, a first fixation agent that is an organic solvent is provided to the cell to change a first characteristic (e.g., cell size) and a second fixation agent that is a cross-linking agent is provided to the cell to change a second characteristic (e.g., cell fluidity or rigidity). The first fixation agent may be provided to the cell before the second fixation agent.
In some instances, an approximately spherical cell may comprise a first diameter prior to being subjected to a first set of conditions (e.g., fixation, such as fixation achieved using an organic solvent) that is reduced in size compared to a second diameter following such processing (e.g., fixation) when maintained in a non-aqueous environment. Following this reduction in size to the second diameter, when maintained in an aqueous environment, the cell may increase in size to have a diameter substantially similar to the first diameter. In some cases, an approximately spherical cell may comprise a first diameter prior to being subjected to the first set of conditions (e.g., fixation, such as fixation achieved using an organic solvent) that is reduced in size compared to a second diameter following subjecting the cell to the first set of conditions. Following this reduction in size to the second diameter, the cell may be cross-linked by a second chemical species (e.g., second fixative), wherein the second diameter is substantially maintained in an aqueous environment following cross-linking by the second chemical species (e.g., second fixative).
A change to a characteristic or set of characteristics of the cell may be reversible or irreversible. In some cases, a change to a characteristic or set of characteristics of the cell may be substantially irreversible, such that the change cannot be readily undone. For example, the size, morphology, or other feature of the cell may be altered in a way that cannot be readily reversed. In an example, the change from a first cross-section of the cell to a second cross-section of the cell is irreversible. In some cases, an irreversible change may be at least partially reversed upon the application of appropriate conditions and/or over a period of time. In other cases, a change to a characteristic or set of characteristics of the cell may be at least partially reversible. For example, the size of a cell may be reduced upon being subjected to a first condition or set of conditions (e.g., fixation using a first chemical species, such as an organic solvent), and the size of a cell may be increased to approximately the original size upon being subjected to a second condition or set of conditions (e.g., rehydration within an aqueous environment). Thus, the change from a first cross-section of the cell to the second cross-section may be reversible. A reversible change (e.g., a reversible size reduction) may be useful in, for example, providing a cell of a given size to a given location, such as a partition. In some cases, a change to a characteristic or set of characteristics of the cell may be only partially reversible. For example, the size of a cell may be reduced (e.g., by dehydration), and the reduction in cell size may be accompanied by reorganization of components within the cell. Upon reversal of the size of the cell (e.g., by rehydration), the arrangement of one or more cellular components may not revert to the original arrangement of the cell prior to the size reduction. In another example, the size of the cell may be reduced. Upon reversal of the size of the cell, the size of the cell may not revert to the original size of the cell prior to the size reduction. A change to a characteristic or set of characteristics may be reversible by at least 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90% or more, wherein the percentage is determined by the percent difference in the characteristics or set of characteristics after reversal compared to the characteristics or set of characteristics prior to being subjected to a first condition and a 100% reversibility indicates a complete reversal to the original characteristics or set of characteristics of the cell. A change to a characteristic or set of characteristics of the cell, such as a cross-section of the cell, may be reversible upon application of a stimulus. The stimulus may be, for example, a thermal stimulus, a photo stimulus, or a chemical stimulus. In some cases, the stimulus may comprise a change in pH and/or application of a reducing agent such as dithiothreitol. Application of the stimulus may reverse, wholly or in part, a change from, for example, a first cross-section to a second cross-section.
In some cases, a polymer or gel matrix may be formed within the cell. The formation of a polymer or gel matrix within the cell may result in a change in one or more characteristics of the cell, such as the size, fluidity, porosity, rigidity, organization, or one or more other features of the cell. A polymer or gel matrix may be formed, for example, upon cross-linking one or more cross-linkable molecules within the cell. For example, a polymer or gel matrix may be formed upon cross-linking one or more molecules within the cell. The polymer or gel matrix may be formed upon polymerizing a plurality of monomers within the cell. For examples, the plurality of monomers may comprise amino acids or nucleic acids. The polymer or gel matrix may be formed upon polymerizing a plurality of polymers within the cell. For example, the polymers may comprise proteins, DNA, or RNA. Polymeric or gel precursors may be provided to the cell and may not form a polymer or gel matrix without application of a stimulus (e.g., as described herein). In some cases, the cell and/or components thereof may be encapsulated (e.g., entrained or entrapped) within the polymer or gel matrix. Formation of a polymer or gel matrix within the cell may take place following one or more other changes to the cell that may be brought about by one or more other conditions.
For example, a first condition or set of conditions may be used to change a first characteristic or set of characteristics of the cell and a polymer or gel matrix may be formed within the cell. The first condition or set of conditions may be used to change a cross-section of the cell from a first cross-section to a second cross-section (e.g., as described herein). In some cases, the cross-section may be changed from a first cross-section to a second cross-section and the polymer or gel matrix may be formed concurrently. The conditions used to bring about these changes may be the same, partially the same, or different. For example, a first condition may be used to bring about the change in the size of the cell and a second condition such as a stimulus (e.g., as described herein) may be used to cause the polymer or gel matrix to form within the cell. Alternatively, a first condition or set of conditions may be used to reduce the size of the cell and cause the polymer or gel matrix to form at the same time. For example, the same chemical species or collection of chemical species may be used to both change the size of the cell and form the polymer or gel matrix.
In some cases, the cross-section may be changed from a first cross-section to a second cross-section and the polymer or gel matrix may be subsequently formed. The conditions used to bring about these changes may be different. For example, a first chemical species or chemical stimulus, optionally in combination with one or more additional conditions, may be used to change the size of the cell and a second chemical species or chemical stimulus, optionally in combination with one or more additional conditions, may be used to form the polymer or gel matrix within the cell.
In some cases, the polymer or gel matrix may be formed, and the cross section of the polymer or gel matrix may be subsequently changed. For example, a first chemical species or stimulus, optionally in combination with one or more additional conditions, may be used to form the polymer or gel matrix, followed by a second chemical species or chemical stimulus, optionally in combination with one or more additional conditions, may be used to change the size of the cell. In some cases, the cell may be lysed when subjected to a chemical species or stimulus, optionally in combination with one or more additional conditions, used to form the polymer or gel matrix. For example, the formation of the polymer network or gel may cause sufficient force on the cell causing it to lyse. In some cases, the cell may be lysed when subjected to a chemical species or stimulus, optionally in combination with one or more additional conditions, used to change the cross-section from a first cross-section to a second cross-section of the polymer or gel matrix. In some cases, the second cross-section may be reduced by at least 6%, 8%, 10%, 15%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, or more relative to the first cross-section. For example, the second cross-section may be reduced by at least about 10%, 15%, 25%, or 50% relative to the first cross-section. In some cases, the cell may be lysed only when subjected to both a first chemical species and a second chemical species used for forming the polymer or gel matrix and for changing the cell cross-section, respectively.
A cell comprising a polymer or gel matrix may be referred to as a cell bead. A cell bead may be prepared by any useful method (e.g., as described herein). Similarly, a particle (e.g., a bead, such as a gel bead) used to process a cell or cell bead or one or more components (e.g., nucleic acid molecules) thereof may be prepared by any useful method. In some cases, preparing a cell bead may comprise combining a cell, such as a cell that has been subjected to conditions to change one or more characteristics of the cell, and one or more polymers and/or polymer precursors and subjecting the combination to a polymerization condition. In an example, the one or more polymers and/or polymer precursors may comprise two different polymers and/or polymer precursors and generating the cell bead comprises subjecting the combination to a condition sufficient to generate a plurality of linkages between the two different polymers and/or polymer precursors. The plurality of linkages may comprise triazole moieties. In such a system, click chemistry may be used to generate the linkages between the two different polymers and/or polymer precursors. For example, the first polymer or polymer precursor may comprise a plurality of azide moieties and the second polymer or polymer precursor may comprise a plurality of alkyne moieties. Reaction between azide and alkyne moieties may be promoted via a copper catalyst or the use of strained alkynes, for example. A particle (e.g., bead) used to process components of a cell or cell bead (e.g., a bead comprising a nucleic acid barcode molecule) may be similarly generated, where one or more polymers or polymer precursors may be linked to the nucleic acid barcode molecule prior to generation of the bead. Alternatively, the nucleic acid barcode molecule may be attached to the bead subsequent to its generation.
Similarly, a bead, a polymer, or polymer precursors (e.g., monomer units that may polymerize and form said polymer) may be coupled to, attached to, functionalized, or otherwise associated with a molecule (e.g., a nucleic acid molecule or a nucleic acid barcode molecule) using conjugation chemistry. Conjugation chemistry as described herein refers to any suitable chemical reaction that links, couples, or attaches a first molecule (e.g., a polymer or a bead) with a second molecule (e.g., a binding agent such as an analyte-specific binding agent, a nucleic acid molecule, a nucleic acid barcode molecule, or any other molecule). Conjugation chemistry may comprise bioconjugation chemistry and click chemistry. Conjugation chemistry may comprise biological interactions (e.g., biotin/strepdavidin interactions) and/or bioorthogonal reactions. In some cases, coupling or attachment of molecules (e.g., nucleic acids, binding agents, etc.) to a bead, cell bead, or polymer as described herein may be performed using click chemistry.
Click chemistry, as described herein, may comprise any type of click reaction suitable for the functionalization of beads, cell beads, polymers, or precursors thereof with various types of molecules such as nucleic acid molecules, nucleic acid barcode molecules, binding agents (e.g., analyte-specific binding agents), etc. Examples of click chemistry reactions (also referred to herein as “click reactions”) that may be used in combination with the methods, systems, and kits provided herein include, but are not limited to, transition-metal catalyzed or strain-promoted azide-alkyne cycloadditions (e.g., Huisgen azide-alkyne 1,3-dipolar cycloaddition, copper-catalyzed azide-alkyne cycloaddition (CuAAC), strain-promoted alkyne-azide cycloaddition, and/or ruthenium-catalyzed azide-alkyne cycloaddition (RuAAC)), Diels-Alder reactions such as inverse-electron demand Diels-Alder reaction (e.g., tetrazine-trans-cyclooctene reactions), or photo-click reactions (e.g., alkene-tetrazole photoreactions).
A bead, cell bead, polymer, or precursor thereof may be attached to one or more sets of molecules (e.g., binding agents, nucleic acid molecules, and/or nucleic acid barcode molecules) using such click chemistry. Thus, a bead, cell bead, polymer, or precursor thereof may comprise (e.g., may be functionalized with) a first functional group. The first functional group may be a first reactant for a click reaction. The one or more sets of molecules (e.g., binding agents and/or nucleic acid molecules) that may be attached to the bead, cell bead, polymer, or precursor thereof, may comprise a second functional group. The second functional group may be a second reactant for a click reaction. The click reaction may be a copper-catalyzed azide-alkyne cycloaddition reaction, an inverse-electron demand Diels-Alder reaction, an avidin-biotin interaction, etc. In an example, a bead, cell bead, polymer, or precursor thereof is modified with an analyte-specific binding agent using a copper-catalyzed azide-alkyne cycloaddition click reaction. Such reaction can comprise an azide-functionalized analyte-specific binding agent and an alkyne-functionalized bead, cell bead, polymer, or precursor thereof (e.g., a cell bead comprising a cell encapsulated in a polymer matric (e.g., a hydrogel matrix)). Click reaction may occur between the azide-functionalized analyte-specific binding agent and alkyne-functionalized bead, cell bead, polymer, or precursor thereof, thereby attaching the binding agent to the bead, cell bead, polymer, or precursor thereof.
In addition to using click chemistry, a molecule (e.g., a binding agent or nucleic acid molecule) can be attached to a bead, cell bead, polymer, or precursor thereof, using various other bioconjugation or coupling methods. Such bioconjugation methods can include various conjugation strategies and functional group modifications such as mesylate formation, sulfur alkylation, NHS ester formation, carbamate formation, carbonate formation, amide bond formation, or any combination thereof. Such strategies and functional group modifications can be used for various reaction types such nucleophilic and/or electrophilic substitution reaction, nucleophilic and/or electrophilic addition reaction, and other suitable reaction types. In some cases, activated carboxylic acids can react with nucleophiles such as amines. In some cases, the carboxylic acid can be attached to a bead, cell bead, polymer, or precursor thereof, and the nucleophilic group such as an amine can be attached to the molecule (e.g., a nucleic acid molecule or a binding agent) to be attached to said bead, cell bead, polymer, or precursor thereof. Such amide bond formation reactions can include EDC/NHS (e.g., via 1-ethyl-3-(3-dimethyl-aminopropyl)carbodiimide (EDC) and N-hydroxysuccinimide (NHS) or 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride (DMTMM)) mediated coupling reactions, wherein an activated ester (e.g., an NHS ester attached to a bead surface) can react with an amine (e.g., an amine of a nucleic acid molecule) to form an amide bond, thereby attaching said molecule (e.g., a nucleic acid molecule) to said bead (e.g., a gel bead). Any other suitable bioconjugation reactions can be used to attach a molecule to a bead.
Subsequent to being subjected to conditions sufficient to change one or more characteristics of a cell (e.g., as described herein), the cell may be provided in an aqueous fluid. In some cases, the cell may be included within an aqueous fluid (e.g., in a solution or emulsion) prior to being subjected to such conditions. In some cases, the cell may undergo one or more changes upon being subjected to such conditions and then undergo one or more further processes such as filtration, extraction, washing, elution, centrifugation, agitation, or isolation. Subsequent to any such processing, the cell may be provided in the aqueous fluid. The aqueous fluid comprising the cell may be contained within a vessel or reservoir. In some cases, the aqueous fluid in which the processed cell is provided may be stored for at least 24 hours, 48 hours, 72 hours, 120 hours, 1 week, 2 weeks, 3 weeks, 4 weeks, 1 month, 2 months, 3 months, 4 months, 5 months, 6 months, 7 months, 8 months, 9 months, 10 months, 11 months, 12 months, or longer. In some cases, one or more stabilizing or preserving agents may be included within the aqueous fluid. Stabilizing and preserving agents may be used to preserve the morphology, size, and other features of the cell. In some cases, the aqueous fluid may be cooled or frozen. In some cases, the aqueous fluid may be used to provide the cell for further processing and analysis. For example, one or more reagents useful for processing or analyzing the cell or components thereof may be added to the aqueous fluid. Reagents useful for processing or analyzing the cell may be selected from the group consisting of lysis agents or buffers, permeabilizing agents, enzymes (e.g., enzymes capable of digesting one or more RNA molecules, extending one or more nucleic acid molecules, reverse transcribing an RNA molecule, permeabilizing or lysing a cell, or carrying out other actions, such as nucleases, restriction enzymes, transposases, polymerases, ligases, exonucleases, reverse transcriptases, and other enzymes), fluorophores, oligonucleotides, primers, barcodes, nucleic acid barcode molecules (e.g., nucleic acid barcode molecules comprising one or more barcode sequences), buffers, deoxynucleotide triphosphates, detergents, reducing agents, chelating agents, oxidizing agents, nanoparticles, beads, and antibodies. In some cases, one or more temperature-sensitive enzymes, pH-sensitive enzymes, light-sensitive enzymes, reverse transcriptases, proteases, ligase, polymerases, restriction enzymes, nucleases, protease inhibitors, exonucleases, and/or nuclease inhibitors may be added to the aqueous fluid. In some cases, the aqueous fluid comprising the cell may be transferred to and/or flowed through a channel of a microfluidic device. The aqueous fluid comprising the cell may be flowed through a junction of two or more channels or expelled through a nozzle to generate a droplet comprising the cell (e.g., as described herein).
In some cases, the aqueous fluid is in a droplet. For example, the aqueous fluid may be in a droplet as part of an emulsion, such as a water-in-oil emulsion. The volume of the droplet may be less than about 100,000 pL. For example, the volume of the droplet may be less than about 10,000 pL, 1,000 pL, 500 pL, 100 pL, 50 pL, or 10 pL. In some cases, the aqueous fluid is a well as part of a plurality of wells. Each well of the plurality of wells may comprise one or more cells (e.g., as described herein). The volume of the well may be less than about 100,000 pL. For example, the volume of the well may be less than about 10,000 pL, 1,000 pL, 500 pL, 100 pL, 50 pL, or 10 pL.
The aqueous fluid comprising the cell may comprise a target molecule of interest. The target molecule may be a nucleic acid molecule that may comprise a sequence of interest. The target nucleic acid molecule may be, for example, a deoxyribonucleic acid (DNA) molecule or a ribonucleic acid (RNA) molecule. Examples of RNA include, but are not limited to, messenger RNA (mRNA), ribosomal RNA (rRNA), mitochondrial RNA (mtRNA), small nucleolar RNA (snoRBA) and transfer RNA (tRNA). The RNA may be a transcript. The RNA may be small RNA that are less than 200 nucleic acid bases in length, or large RNA that are greater than 200 nucleic acid bases in length. Small RNAs may include 5.8S ribosomal RNA (rRNA), 5S rRNA, transfer RNA (tRNA), microRNA (miRNA), small interfering RNA (siRNA), small nucleolar RNA (snoRNAs), Piwi-interacting RNA (piRNA), tRNA-derived small RNA (tsRNA) and small rDNA-derived RNA (srRNA). The RNA may be double-stranded RNA or single-stranded RNA. The RNA may be circular RNA. Similarly, DNA may be double-stranded DNA or single-stranded DNA. The DNA may comprise chromatin. A nucleic acid molecule of interest may have any useful length, base composition, and other characteristics. In some cases, the aqueous fluid comprises an aqueous reaction mixture and the cell comprises a target molecule, and the method comprises performing one or more reactions using the target molecule. In some cases, one or more such reactions are performed within a partition (e.g., as described herein).
In some cases, a target molecule of interest (e.g., a nucleic acid molecule) may be included within the cell. For example, the target molecule of interest may be included in a nucleus of a cell. Access to a target molecule included in the cell may be provided by lysing or permeabilizing the cell. Lysing the cell may release the target molecule contained therein from a cell. The cell may be lysed using a lysis agent such as a bioactive agent. A bioactive agent useful for lysing the cell may be, for example, an enzyme (e.g., as described herein). An enzyme used to lyse the cell may or may not be capable of carrying out additional actions such as degrading one or more RNA molecules. Alternatively, an ionic, zwitterionic, or non-ionic surfactant may be used to lyse the cell. Examples of surfactants include, but are not limited to, TritonX-100, Tween 20, sarcosyl, or sodium dodecyl sulfate. Cell lysis may also be achieved using a cellular disruption method such as an electroporation or a thermal, acoustic, or mechanical disruption method. Alternatively, the cell may be permeabilized to provide access to a target molecule included therein. Permeabilization may involve partially or completely dissolving or disrupting a cell membrane or a portion thereof. Permeabilization may be achieved by, for example, contacting a cell membrane with an organic solvent or a detergent such as Triton X-100 or NP-40.
The cell may be partitioned within a partition such as a well or droplet, e.g., as described herein. For example, the cell provided within the aqueous fluid may be partitioned using a fluid that is immiscible with the aqueous fluid. One or more reagents may be co-partitioned with the cell. For example, the cell may be co-partitioned with one or more reagents selected from the group consisting of lysis agents or buffers, permeabilizing agents, enzymes (e.g., enzymes capable of digesting one or more RNA molecules, extending one or more nucleic acid molecules, reverse transcribing an RNA molecule, permeabilizing or lysing a cell, or carrying out other actions), fluorophores, oligonucleotides, primers, barcodes, nucleic acid barcode molecules (e.g., nucleic acid barcode molecules comprising one or more barcode sequences), buffers, deoxynucleotide triphosphates, detergents, reducing agents, chelating agents, oxidizing agents, nanoparticles, beads, and antibodies. In some cases, the cell may be co-partitioned with one or more reagents selected from the group consisting of temperature-sensitive enzymes, pH-sensitive enzymes, light-sensitive enzymes, reverse transcriptases, proteases, ligase, polymerases, restriction enzymes, nucleases, protease inhibitors, exonucleases, transposases, and nuclease inhibitors. Similarly, the cell may include or be co-partitioned with one or more target molecules. In some cases, the partition comprises an aqueous reaction mixture and the cell comprises a target molecule, and the method comprises performing one or more reactions using the target molecule. In an example, the partition comprises an aqueous reaction mixture comprising a plurality of nucleic acid barcode molecules comprising a common barcode sequence. Partitioning the cell and one or more reagents may comprise flowing a first phase comprising an aqueous fluid, the cell, and the one or more reagents and a second phase comprising a fluid that is immiscible with the aqueous fluid toward a junction. Upon interaction of the first and second phases, a discrete droplet of the first phase comprising the cell and the one or more reagents may be formed. In some cases, the partition may comprise a single cell. The cell may be lysed or permeabilized within the partition (e.g., droplet) to provide access to a target molecule of interest within the cell. Accordingly, molecules originating from the same cell may be isolated within the same partition.
A partition (e.g., droplet in emulsion) comprising a cell, or gel matrix derived thereof, that has been subjected to one or more conditions or sets of conditions described herein may have a first cross-section that is smaller than a second cross-section of a partition comprising a cell, or gel matrix derived thereof, that has not been subjected to one or more conditions or sets of conditions described herein. In some cases, the first cross-section may be smaller by at least 6%, 8%, 10%, 15%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, or more relative to the second cross-section. For example, the first cross-section may be smaller by at least about 10%, 15%, 25%, or 50% relative to the second cross-section.
In some cases, the methods of the present disclosure may be performed within a partition. For example, the cell may be provided within a partition (e.g., a droplet or well) for processing. The cell may be subjected to conditions sufficient to change one or more characteristics of the cell (e.g., as described herein). For example, a cross-section of the cell may be changed from a first cross-section to a second cross-section within the partition. Similarly, a polymer or gel matrix may be formed within the cell within the partition.
A partition (e.g., a well or droplet) comprising the cell may further comprise a bead (e.g., as described herein). In some instances, a cell may be introduced to a partition comprising a bead (e.g., a gel bead).
In some cases, a bead may be subjected to processing as described elsewhere herein with regard to cells. For example, the bead may be subjected to processing prior to partitioning into a partition. Alternatively or in addition, the bead may be subjected to processing during or subsequent to partitioning in the partition. In some cases, one or more physical parameters or dimensions (e.g., volume and diameter) and/or one or more other characteristics of the bead may be changed. For example, a cross-section of the bead may be changed from a first cross-section to a second cross-section. The first cross-section may be smaller or larger than the second cross-section. In some cases, the second cross-section may be reduced by at least 6%, 8%, 10%, 15%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, or more relative to the first cross-section. For example, the second cross-section may be reduced by at least about 10%, 15%, 25%, or 50% relative to the first cross-section. Alternatively or in addition, one or more other characteristics of the bead may be changed. For example, the fluidity, density, rigidity, porosity, refractive index, polarity, or other characteristic of the bead or one or more components thereof may be changed.
A characteristic or set of characteristics of the bead may be changed by one or more conditions. A condition suitable for changing a characteristic or set of characteristics of the bead may be, for example, a temperature, a pH, an ion or salt concentration, a pressure, or another condition. For example, the bead may be exposed to a chemical species that may bring about a change in one or more characteristics of the bead. In some cases, a stimulus may be used to change one or more characteristics of the bead. For example, upon application of the stimulus, one or more characteristics of the bead may be changed. The stimulus may be, for example, a thermal stimulus, a photo stimulus, a chemical stimulus, or another stimulus. In some cases, conditions sufficient to change the one or more characteristics of the bead may comprise one or more different conditions, such as a temperature and a pressure, a pH and a salt concentration, a chemical species and a temperature, or any other combination of conditions. A pH sufficient for changing one or more characteristics of the bead may be, for example, between about 5 and 8, such as between about 6 and 7.
In some cases, a temperature change may be used to change the characteristics of the bead. The bead may comprise a temperature responsive polymer such as Poly-N (isopropylacrylamide). For example, changing the temperature may result in changing a dimension of the bead. A temperature sufficient for changing one or more characteristics of the cell may be, for example, at least about 0 degrees Celsius (° C.), 1° C., 2° C., 3° C., 4° C., 5° C., 10° C., or higher. For example, the temperature may be about 4° C. In other cases, a temperature sufficient for changing one or more characteristics of the cell may be, for example, at least about 25° C., 30° C., 35° C., 37° C., 40° C., 45° C., 50° C., or higher. For example, the temperature may be about 37° C.
In some cases, a change to one or more characteristics of the bead may be mediated by a chemical species or a chemical stimulus. For example, a chemical species or chemical stimulus may be used to change a dimension of the bead (e.g., a cross-section, diameter, or volume). The chemical species may be one of those used for processing cells as described elsewhere herein. In some cases, the chemical species is a fixation agent as described elsewhere herein.
A partition (e.g., droplet in emulsion) comprising a bead that has been subjected to one or more conditions or sets of conditions described herein may have a first cross-section that is smaller than a second cross-section of a partition comprising a bead that has not been subjected to one or more conditions or sets of conditions described herein. In some cases, the first cross-section may be smaller by at least 6%, 8%, 10%, 15%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, or more relative to the second cross-section. For example, the first cross-section may be smaller by at least about 10%, 15%, 25%, or 50% relative to the second cross-section.
A first partition (e.g., droplet in emulsion) comprising both a cell (e.g., a cell processed as described herein, such as a cell bead comprising a polymer or gel matrix) and a bead that has been processed to change one or more of its characteristics (e.g., as described herein) may have a first cross-section that is smaller than a second cross-section of a second partition comprising both a cell (e.g., a cell processed as described herein, such as a cell bead comprising a polymer or gel matrix) and a bead that has not been process to change one or more of its characteristics. In some cases, the first cross-section may be smaller by at least 6%, 8%, 10%, 15%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, or more relative to the second cross-section. For example, the first cross-section may be smaller by at least about 10%, 15%, 25%, or 50% relative to the second cross-section.
In a first operation 1150, the first particle 1102A and the second particle 1104A may be subjected to one or more conditions or one or more sets of conditions described herein to reduce a first cross-section of the first particle and a second cross-section of the second particle to generate a reduced first particle 1102B and a reduced second particle 1104B, respectively. In a second operation, the 1160, the reduced first particle 1102B and the reduced second particle 1104B may be co-partitioned in a partition 1106 (e.g., a droplet or well). The partition 1106 may have a cross-section smaller than a partition that would comprise the first particle 1102A and the second particle 1104A, prior to application of the one or more conditions or the one or more sets of conditions.
Alternatively, one or more conditions or one or more sets of conditions may be applied to only one of the particles (e.g., cells and/or beads), and not both. The resulting partition may still have a cross-section that is smaller than a partition that would comprise the first particle and the second particle prior to application of the one or more conditions or the one or more sets of conditions.
Alternatively, a single particle may be provided, one or more conditions or one or more sets of conditions may be applied to the single particle and the single particle partitioned. The partition may have a cross-section that is smaller than a partition that would comprise the single particle prior to application of the one or more conditions or the one or more sets of conditions. In some cases, a second particle may be delivered to the partition (e.g., subsequent to provision of the first particle within the partition).
Alternatively, the first particle and the second particle, prior to application of the one or more conditions or one or more sets of conditions, may be partitioned. The one or more conditions or one or more sets of conditions may be applied subsequent to the partitioning to reduce cross-sections of the particles. Alternatively, the one or more conditions or one or more sets of conditions may be applied during partitioning. For example, operations 1150 and 1160 may be performed simultaneously or substantially simultaneously, whether with both particles or with only one of the particles.
In some cases, the one or more conditions or one or more sets of conditions to reduce particle cross-section may be applied to the two particles at different times. Alternatively, one or more conditions or one or more sets of conditions to reduce particle cross-section may be applied simultaneously.
A bead processed according to the methods provided herein and/or used in the processing of a cell or component thereof may be a gel bead. The bead may comprise a plurality of nucleic acid barcode molecules (e.g., nucleic acid molecules each comprising a sequence comprising one or more barcode sequences, as described herein). The bead may comprise at least 10,000 nucleic acid barcode molecules attached thereto. For example, the bead may comprise at least 100,000, 1,000,000, or 10,000,000 nucleic acid barcode molecules attached thereto. Sequences of the plurality of nucleic acid barcode molecules may be releasably attached to the bead. The sequences of the plurality of nucleic acid barcode molecules may be releasable from the bead upon application of a stimulus. Such a stimulus may be selected from the group consisting of a thermal stimulus, a photo stimulus, and a chemical stimulus. For example, the stimulus may be a reducing agent such as dithiothreitol Application of a stimulus may result in one or more of (i) cleavage of a linkage between the sequences of the nucleic acid barcode molecules of the plurality of nucleic acid barcode molecules and the bead, and (ii) degradation or dissolution of the bead to release sequences of the nucleic acid barcode molecules of the plurality of nucleic acid barcode molecules from the bead. In some cases, the nucleic acid barcode molecules may be released from the bead (e.g., upon application of a stimulus).
The plurality of nucleic acid barcode molecules attached to a bead within a partition (e.g., a well or droplet) comprising the cell may be useful in a reaction involving a target molecule (e.g., target nucleic acid molecule) of the cell. For example, a nucleic acid barcode molecule may barcode the target molecule, e.g., upon attachment by a hybridization or ligation process. In some cases, the plurality of nucleic acid barcode molecules attached to the bead may comprise a common barcode sequence. The plurality of nucleic acid barcode molecules may also comprise one or more functional sequences selected from the group consisting of a primer sequence, a primer annealing sequence, and an immobilization sequence. Nucleic acid barcode molecules comprising a primer sequence may be useful in generating one or more complements or copies of one or more nucleic acid molecules (e.g., RNA or cDNA molecules) that may be included within the cell. For example, one or more nucleic acid molecules included within the cell within a partition (e.g., as described herein) may be subjected to conditions suitable for performing one or more primer extension reactions, thereby generating extension products, where the extension reactions comprise annealing a primer sequence of one or more of the nucleic acid barcode molecules to the nucleic acid molecules. Access to the nucleic acid molecules of the cell may be facilitated by lysing or permeabilizing the cell (e.g., as described herein). Prior to performing the extension reactions, sequences of nucleic acid barcode molecules of the plurality of nucleic acid barcode molecules may be released from the bead upon application of a stimulus to enhance the probability of interaction between the sequences of the nucleic acid barcode molecules and the nucleic acid molecules of the cell. In some cases, one or more amplification reactions (e.g., PCR) may be performed to generate amplification products.
In an example, a cell is provided within a partition (e.g., a droplet or well). The cell within the partition is then subjected to a first condition or set of conditions sufficient to change a cross-section of the cell from a first cross-section to a second cross-section, which second cross-section is less than the first cross-section. The first condition or set of conditions may comprise, for example, a temperature condition, a pH condition, a chemical condition, or a pressure condition (e.g., as described herein). For example, the first condition or set of conditions may comprise a chemical species such as an organic solvent (e.g., acetone, methanol, or ethanol). The cell may comprise target molecule that may be a nucleic acid molecule. The processed cell may subsequently be subjected to a second condition or set of conditions sufficient to form crosslinks within the cell to yield a crosslinked cell. The second condition or set of conditions may comprise, for example, a temperature condition, a pH condition, a chemical condition, or a pressure condition (e.g., as described herein). For example, the second condition or set of conditions may comprise a chemical species such as a cross-linking agent (e.g., a photocleavable cross-linker). In some cases, the chemical species may be selected from the group consisting of dithio-bis(-succinimidyl propionate) (DSP), disuccinimidyl tartrate (DST), and ethylene glycol bis(succinimidyl succinate) (EGS). The partition including the cell may comprise one or more other reagents (e.g., as described elsewhere herein). For example, the partition may comprise one or more reagents for lysing or permeabilizing the cell to provide access to the target molecule; one or more nucleic acid barcode molecules capable of barcoding the target molecule of the cell; and one or more other reagents.
In another example, a cell is subjected to a first condition or set of conditions sufficient to change a cross-section of the cell from a first cross-section to a second cross-section, which second cross-section is less than the first cross-section. The first condition or set of conditions may comprise, for example, a temperature condition, a pH condition, a chemical condition, or a pressure condition (e.g., as described herein). For example, the first condition or set of conditions may comprise a chemical species such as an organic solvent (e.g., acetone, methanol, or ethanol). The cell may comprise target molecule that may be a nucleic acid molecule. The processed cell may subsequently be subjected to a second condition or set of conditions sufficient to form crosslinks within the cell to yield a crosslinked cell. The second conditions or set of conditions may comprise, for example, a temperature condition, a pH condition, a chemical condition, or a pressure condition. For example, the second condition or set of conditions may comprise a chemical species such as a cross-linking agent (e.g., a photocleavable cross-linker). In some cases, the chemical species may be selected from the group consisting of dithio-bis(-succinimidyl propionate) (DSP), disuccinimidyl tartrate (DST), and ethylene glycol bis(succinimidyl succinate) (EGS). The cell may be provided in an aqueous fluid. The aqueous fluid comprising the cell may then be provided within a channel of a microfluidic device to a droplet generation junction and a droplet comprising the cell may be generated. The cell may then undergo further processing. The cell may be co-partitioned with one or more other reagents (e.g., as described herein). For example, the partition (e.g., an aqueous droplet in an emulsion or a well) may comprise one or more reagents for lysing or permeabilizing the cell to provide access to the target molecule; one or more nucleic acid barcode molecules capable of barcoding the target molecule of the cell; and one or more other reagents. The cell may be lysed or permeabilized to provide access to a target molecule included therein. The cell may be co-partitioned with a bead (e.g., a gel bead) comprising a plurality of nucleic acid barcode molecules attached thereto. The plurality of nucleic acid barcode molecules may comprise a common barcode sequence. The plurality of nucleic acid barcode molecules may also comprise one or more additional sequences as described further herein including, but not limited to, flow cell sequences, primer sequences, sequencing primer sequences, sequences capable of coupling to target molecule (e.g., random N-mers or poly-T sequences) unique molecular identifiers, and/or other sequences. Sequences of the plurality of nucleic acid barcode molecules may be releasably attached to the bead and may be releasable from the bead upon application of a stimulus (e.g., as described herein). One or more nucleic acid barcode molecules of the plurality of nucleic acid barcode molecules or one or more sequences thereof may participate in a reaction with the target molecule. For example, a nucleic acid barcode molecule or sequence thereof may hybridize to the target molecule and undergo an extension reaction, thereby generating a complement of at least a portion of the target molecule. In other cases, a nucleic acid barcode molecule or sequence thereof may be coupled to the target molecule by a ligation reaction, thereby generating a barcoded target molecule. Extension reactions may then be performed to generate a complement of the target molecule, or a portion thereof. In some cases, amplification reactions such as polymerase chain reactions (PCR) may be performed to generate copies of the barcoded target molecule and/or its complement. The generation of one or more copies or complements of a sequence of interest of a target molecule, or a portion thereof, (e.g., via one or more extension and/or amplification reactions) may facilitate, for example, detection of the sequence of interest (e.g., using sequencing).
The methods described herein may be applied to a single cell or a plurality of cells. In some cases, the plurality of cells is a suspension of cells. In some cases, at least one step of the methods of the present invention is performed on a single cell or a plurality of cells outside of any partition. For instance, a method of processing a plurality of cells may comprise providing the plurality of cells within a vessel and subjecting the plurality of cells to conditions sufficient to change one or more characteristics of the cell. For example, the plurality of cells may be subjected to a first condition or set of conditions comprising a chemical species, and a cross-section of the cells of the plurality of cells may change from a first cross-section to a second cross-section, which second cross-section is less than the first cross-section. The chemical species may comprise, for example, an organic solvent such as ethanol, methanol, or acetone. The plurality of cells may then be subjected to a second condition or set of conditions comprising a chemical species, and crosslinks may form within each of the cells. The chemical species may comprise, for example, a cross-linking agent. The plurality of processed cells may be provided in an aqueous fluid. In some instances, the second cross-section of the plurality of cells is substantially maintained in the aqueous fluid. The plurality of processed cells may be partitioned within a plurality of partitions. The partitions may be, for example, aqueous droplets included in a water-in-oil emulsion. The partitions may be, for example, a plurality of wells. The plurality of fixed cells may be co-partitioned with one or more reagents. In some cases, the plurality of fixed cells may be co-partitioned with one or more beads, where each bead comprises a plurality of nucleic acid barcode molecules attached thereto. The nucleic acid barcode molecules attached to a given bead may comprise a common barcode sequence, and the nucleic acid barcode molecules attached to each different bead may comprise a sequence comprising a different common barcode sequence. The nucleic acid barcode molecules, or portions thereof, may then be used in reactions with target molecules associated with cells of the plurality of cells.
Systems and Methods for Sample Compartmentalization
In an aspect, the systems and methods described herein provide for the compartmentalization, depositing, or partitioning of one or more particles (e.g., biological particles, macromolecular constituents of biological particles, beads, reagents, etc.) into discrete compartments or partitions (referred to interchangeably herein as partitions), where each partition maintains separation of its own contents from the contents of other partitions. The partition can be a droplet in an emulsion. A partition may comprise one or more other partitions.
A partition may include one or more particles. A partition may include one or more types of particles. For example, a partition of the present disclosure may comprise one or more biological particles and/or macromolecular constituents thereof. A partition may comprise one or more gel beads. A partition may comprise one or more cell beads. A partition may include a single gel bead, a single cell bead, or both a single cell bead and single gel bead. A partition may include one or more reagents. Alternatively, a partition may be unoccupied. For example, a partition may not comprise a bead. A cell bead can be a biological particle and/or one or more of its macromolecular constituents encased inside of a gel or polymer matrix, such as via polymerization of a droplet containing the biological particle and precursors capable of being polymerized or gelled. Unique identifiers, such as barcodes, may be injected into the droplets previous to, subsequent to, or concurrently with droplet generation, such as via a microcapsule (e.g., bead), as described elsewhere herein. Microfluidic channel networks (e.g., on a chip) can be utilized to generate partitions as described herein. Alternative mechanisms may also be employed in the partitioning of individual biological particles, including porous membranes through which aqueous mixtures of cells are extruded into non-aqueous fluids.
The partitions can be flowable within fluid streams. The partitions may comprise, for example, micro-vesicles that have an outer barrier surrounding an inner fluid center or core. In some cases, the partitions may comprise a porous matrix that is capable of entraining and/or retaining materials within its matrix. The partitions can be droplets of a first phase within a second phase, wherein the first and second phases are immiscible. For example, the partitions can be droplets of aqueous fluid within a non-aqueous continuous phase (e.g., oil phase). In another example, the partitions can be droplets of a non-aqueous fluid within an aqueous phase. In some examples, the partitions may be provided in a water-in-oil emulsion or oil-in-water emulsion. A variety of different vessels are described in, for example, U.S. Patent Application Publication No. 2014/0155295, which is entirely incorporated herein by reference for all purposes. Emulsion systems for creating stable droplets in non-aqueous or oil continuous phases are described in, for example, U.S. Patent Application Publication No. 2010/0105112, which is entirely incorporated herein by reference for all purposes.
In the case of droplets in an emulsion, allocating individual particles to discrete partitions may in one non-limiting example be accomplished by introducing a flowing stream of particles in an aqueous fluid into a flowing stream of a non-aqueous fluid, such that droplets are generated at the junction of the two streams. Fluid properties (e.g., fluid flow rates, fluid viscosities, etc.), particle properties (e.g., volume fraction, particle size, particle concentration, etc.), microfluidic architectures (e.g., channel geometry, etc.), and other parameters may be adjusted to control the occupancy of the resulting partitions (e.g., number of biological particles per partition, number of beads per partition, etc.). For example, partition occupancy can be controlled by providing the aqueous stream at a certain concentration and/or flow rate of particles. To generate single biological particle partitions, the relative flow rates of the immiscible fluids can be selected such that, on average, the partitions may contain less than one biological particle per partition in order to ensure that those partitions that are occupied are primarily singly occupied. In some cases, partitions among a plurality of partitions may contain at most one biological particle (e.g., bead, DNA, cell or cellular material). In some embodiments, the various parameters (e.g., fluid properties, particle properties, microfluidic architectures, etc.) may be selected or adjusted such that a majority of partitions are occupied, for example, allowing for only a small percentage of unoccupied partitions. The flows and channel architectures can be controlled as to ensure a given number of singly occupied partitions, less than a certain level of unoccupied partitions and/or less than a certain level of multiply occupied partitions.
The second fluid 116 can comprise an oil, such as a fluorinated oil, that includes a fluorosurfactant for stabilizing the resulting droplets, for example, inhibiting subsequent coalescence of the resulting droplets 118, 120. Examples of particularly useful partitioning fluids and fluorosurfactants are described, for example, in U.S. Patent Application Publication No. 2010/0105112, which is entirely incorporated herein by reference for all purposes.
As will be appreciated, the channel segments described herein may be coupled to any of a variety of different fluid sources or receiving components, including reservoirs, tubing, manifolds, or fluidic components of other systems. As will be appreciated, the microfluidic channel structure 100 may have other geometries. For example, a microfluidic channel structure can have more than one channel junction. For example, a microfluidic channel structure can have 2, 3, 4, or 5 channel segments each carrying particles (e.g., biological particles, cell beads, and/or gel beads) that meet at a channel junction. Fluid may be directed to flow along one or more channels or reservoirs via one or more fluid flow units. A fluid flow unit can comprise compressors (e.g., providing positive pressure), pumps (e.g., providing negative pressure), actuators, and the like to control flow of the fluid. Fluid may also or otherwise be controlled via applied pressure differentials, centrifugal force, electrokinetic pumping, vacuum, capillary or gravity flow, or the like.
The generated droplets may comprise two subsets of droplets: (1) occupied droplets 118, containing one or more biological particles 114, and (2) unoccupied droplets 120, not containing any biological particles 114. Occupied droplets 118 may comprise singly occupied droplets (having one biological particle) and multiply occupied droplets (having more than one biological particle). As described elsewhere herein, in some cases, the majority of occupied partitions can include no more than one biological particle per occupied partition and some of the generated partitions can be unoccupied (of any biological particle). In some cases, though, some of the occupied partitions may include more than one biological particle. In some cases, the partitioning process may be controlled such that fewer than about 25% of the occupied partitions contain more than one biological particle, and in many cases, fewer than about 20% of the occupied partitions have more than one biological particle, while in some cases, fewer than about 10% or even fewer than about 5% of the occupied partitions include more than one biological particle per partition.
In some cases, it may be desirable to minimize the creation of excessive numbers of empty partitions, such as to reduce costs and/or increase efficiency. While this minimization may be achieved by providing a sufficient number of biological particles (e.g., biological particles 114) at the partitioning junction 110, such as to ensure that at least one biological particle is encapsulated in a partition, the Poissonian distribution may expectedly increase the number of partitions that include multiple biological particles. As such, where singly occupied partitions are to be obtained, at most about 95%, 90%, 85%, 80%, 75%, 70%, 65%, 60%, 55%, 50%, 45%, 40%, 35%, 30%, 25%, 20%, 15%, 10%, 5% or less of the generated partitions can be unoccupied.
In some cases, the flow of one or more of the biological particles (e.g., in channel segment 102), or other fluids directed into the partitioning junction (e.g., in channel segments 104, 106) can be controlled such that, in many cases, no more than about 50% of the generated partitions, no more than about 25% of the generated partitions, or no more than about 10% of the generated partitions are unoccupied. These flows can be controlled so as to present a non-Poissonian distribution of single-occupied partitions while providing lower levels of unoccupied partitions. The above noted ranges of unoccupied partitions can be achieved while still providing any of the single occupancy rates described above. For example, in many cases, the use of the systems and methods described herein can create resulting partitions that have multiple occupancy rates of less than about 25%, less than about 20%, less than about 15%, less than about 10%, and in many cases, less than about 5%, while having unoccupied partitions of less than about 50%, less than about 40%, less than about 30%, less than about 20%, less than about 10%, less than about 5%, or less.
As will be appreciated, the above-described occupancy rates are also applicable to partitions that include both biological particles and additional reagents, including, but not limited to, microcapsules or beads (e.g., gel beads) carrying barcoded nucleic acid molecules (e.g., oligonucleotides) (described in relation to
In another aspect, in addition to or as an alternative to droplet based partitioning, biological particles may be encapsulated within a microcapsule that comprises an outer shell, layer or porous matrix in which is entrained one or more individual biological particles or small groups of biological particles. The microcapsule may include other reagents. Encapsulation of biological particles may be performed by a variety of processes. Such processes may combine an aqueous fluid containing the biological particles with a polymeric precursor material that may be capable of being formed into a gel or other solid or semi-solid matrix upon application of a particular stimulus to the polymer precursor. Such stimuli can include, for example, thermal stimuli (e.g., either heating or cooling), photo-stimuli (e.g., through photo-curing), chemical stimuli (e.g., through crosslinking, polymerization initiation of the precursor (e.g., through added initiators)), mechanical stimuli, or a combination thereof.
Preparation of microcapsules comprising biological particles may be performed by a variety of methods. For example, air knife droplet or aerosol generators may be used to dispense droplets of precursor fluids into gelling solutions in order to form microcapsules that include individual biological particles or small groups of biological particles. Likewise, membrane based encapsulation systems may be used to generate microcapsules comprising encapsulated biological particles as described herein. Microfluidic systems of the present disclosure, such as that shown in
For example, in the case where the polymer precursor material comprises a linear polymer material, such as a linear polyacrylamide, PEG, or other linear polymeric material, the activation agent may comprise a cross-linking agent, or a chemical that activates a cross-linking agent within the formed droplets. Likewise, for polymer precursors that comprise polymerizable monomers, the activation agent may comprise a polymerization initiator. For example, in certain cases, where the polymer precursor comprises a mixture of acrylamide monomer with a N,N′-bis-(acryloyl)cystamine (BAC) comonomer, an agent such as tetraethylmethylenediamine (TEMED) may be provided within the second fluid streams 116 in channel segments 104 and 106, which can initiate the copolymerization of the acrylamide and BAC into a cross-linked polymer network, or hydrogel.
Upon contact of the second fluid stream 116 with the first fluid stream 112 at junction 110, during formation of droplets, the TEMED may diffuse from the second fluid 116 into the aqueous fluid 112 comprising the linear polyacrylamide, which will activate the crosslinking of the polyacrylamide within the droplets 118, 120, resulting in the formation of gel (e.g., hydrogel) microcapsules, as solid or semi-solid beads or particles entraining the cells 114. Although described in terms of polyacrylamide encapsulation, other ‘activatable’ encapsulation compositions may also be employed in the context of the methods and compositions described herein. For example, formation of alginate droplets followed by exposure to divalent metal ions (e.g., Ca2+ ions), can be used as an encapsulation process using the described processes. Likewise, agarose droplets may also be transformed into capsules through temperature based gelling (e.g., upon cooling, etc.).
In some cases, encapsulated biological particles can be selectively releasable from the microcapsule, such as through passage of time or upon application of a particular stimulus, that degrades the microcapsule sufficiently to allow the biological particles (e.g., cell), or its other contents to be released from the microcapsule, such as into a partition (e.g., droplet). For example, in the case of the polyacrylamide polymer described above, degradation of the microcapsule may be accomplished through the introduction of an appropriate reducing agent, such as DTT or the like, to cleave disulfide bonds that cross-link the polymer matrix. See, for example, U.S. Patent Application Publication No. 2014/0378345, which is entirely incorporated herein by reference for all purposes.
The biological particle can be subjected to other conditions sufficient to polymerize or gel the precursors. The conditions sufficient to polymerize or gel the precursors may comprise exposure to heating, cooling, electromagnetic radiation, and/or light. The conditions sufficient to polymerize or gel the precursors may comprise any conditions sufficient to polymerize or gel the precursors. Following polymerization or gelling, a polymer or gel may be formed around the biological particle. The polymer or gel may be diffusively permeable to chemical or biochemical reagents. The polymer or gel may be diffusively impermeable to macromolecular constituents of the biological particle. In this manner, the polymer or gel may act to allow the biological particle to be subjected to chemical or biochemical operations while spatially confining the macromolecular constituents to a region of the droplet defined by the polymer or gel. The polymer or gel may include one or more of disulfide cross-linked polyacrylamide, agarose, alginate, polyvinyl alcohol, polyethylene glycol (PEG)-diacrylate, PEG-acrylate, PEG-thiol, PEG-azide, PEG-alkyne, other acrylates, chitosan, hyaluronic acid, collagen, fibrin, gelatin, or elastin. The polymer or gel may comprise any other polymer or gel.
The polymer or gel may be functionalized to bind to targeted analytes, such as nucleic acids, proteins, carbohydrates, lipids or other analytes. The polymer or gel may be polymerized or gelled via a passive mechanism. The polymer or gel may be stable in alkaline conditions or at elevated temperature. The polymer or gel may have mechanical properties similar to the mechanical properties of the bead. For instance, the polymer or gel may be of a similar size to the bead. The polymer or gel may have a mechanical strength (e.g. tensile strength) similar to that of the bead. The polymer or gel may be of a lower density than an oil. The polymer or gel may be of a density that is roughly similar to that of a buffer. The polymer or gel may have a tunable pore size. The pore size may be chosen to, for instance, retain denatured nucleic acids. The pore size may be chosen to maintain diffusive permeability to exogenous chemicals such as sodium hydroxide (NaOH) and/or endogenous chemicals such as inhibitors. The polymer or gel may be biocompatible. The polymer or gel may maintain or enhance cell viability. The polymer or gel may be biochemically compatible. The polymer or gel may be polymerized and/or depolymerized thermally, chemically, enzymatically, and/or optically.
The polymer may comprise poly(acrylamide-co-acrylic acid) crosslinked with disulfide linkages. The preparation of the polymer may comprise a two-step reaction. In the first activation step, poly(acrylamide-co-acrylic acid) may be exposed to an acylating agent to convert carboxylic acids to esters. For instance, the poly(acrylamide-co-acrylic acid) may be exposed to 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride (DMTMM). The polyacrylamide-co-acrylic acid may be exposed to other salts of 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium. In the second cross-linking step, the ester formed in the first step may be exposed to a disulfide crosslinking agent. For instance, the ester may be exposed to cystamine (2,2′-dithiobis(ethylamine)). Following the two steps, the biological particle may be surrounded by polyacrylamide strands linked together by disulfide bridges. In this manner, the biological particle may be encased inside of or comprise a gel or matrix (e.g., polymer matrix) to form a “cell bead.” A cell bead can contain biological particles (e.g., a virus, a cell, or a cell nucleus) or macromolecular constituents (e.g., RNA, DNA, proteins, etc.) of biological particles. For example after lysing and washing the cells, inhibitory components from cell lysates can be washed away and the macromolecular constituents can be bound as cell beads. Systems and methods disclosed herein can be applicable to both cell beads (and/or droplets or other partitions) containing biological particles and cell beads (and/or droplets or other partitions) containing macromolecular constituents of biological particles.
The polymer may be responsive to temperature. For example, the cell bead may comprise the polymer and when heated or cooled, the characteristics or dimensions of the cell bead may change. For example, the polymer may comprise poly (N-isopropylacrylamide). The cell bead may comprise poly (N-isopropylacrylamide) and when heated the cell bead may decrease in one or more dimensions (e.g., a cross-sectional diameter, multiple cross-sectional diameters). A temperature sufficient for changing one or more characteristics of the cell bead may be, for example, at least about 0 degrees Celsius (° C.), 1° C., 2° C., 3° C., 4° C., 5° C., 10° C., or higher. For example, the temperature may be about 4° C. In other cases, a temperature sufficient for changing one or more characteristics of the cell may be, for example, at least about 25° C., 30° C., 35° C., 37° C., 40° C., 45° C., 50° C., or higher. For example, the temperature may be about 37° C.
A cell bead may include a single cell or multiple cells, or a derivative of the single cell or multiple cells (e.g., multiple cells adhered together). A cell bead may include any type of cell, including without limitation prokaryotic cells, eukaryotic cells, bacterial, fungal, plant, mammalian, or other animal cell types, mycoplasmas, normal tissue cells, tumor cells, a T-cell (e.g., CD4 T-cell, CD4 T-cell that comprises a dormant copy of human immunodeficiency virus (HIV)), a fixed cell, a cross-linked cell, a rare cell from a population of cells, or any other cell type, whether derived from single cell or multicellular organisms. Furthermore, a cell bead may comprise a live cell, such as, for example, a cell may be capable of being cultured. Moreover, in some examples, a cell bead may comprise a derivative of a cell, such as one or more components of the cell (e.g., an organelle, a cell protein, a cellular nucleic acid, genomic nucleic acid, messenger ribonucleic acid, a ribosome, a cellular enzyme, etc.). In some examples, a cell bead may comprise material obtained from a biological tissue, such as, for example, obtained from a subject. In some cases, cells, viruses or macromolecular constituents thereof are encapsulated within a cell bead. Encapsulation can be within a polymer or gel matrix that forms a structural component of the cell bead. In some cases, a cell bead is generated by fixing a cell in a fixation medium or by cross-linking elements of the cell, such as the cell membrane, the cell cytoskeleton, etc. In some cases, beads may or may not be generated without encapsulation within a larger cell bead.
The cell beads may also be subjected to processing as was applied to cells described elsewhere herein. In some cases, one or more physical parameters or dimensions (e.g., diameter and volume) and/or one or more other characteristics of the cell bead may be changed. For example, a cross-section of the cell bead may be changed from a first cross-section to a second cross-section. The first cross-section may be smaller or larger than the second cross-section. Alternatively or in addition, one or more other characteristics of the cell bead may be changed. For example, the fluidity, density, rigidity, porosity, or other characteristic of the cell bead or one or more components thereof may be changed.
Encapsulated biological particles can provide certain potential advantages of being more storable and more portable than droplet-based partitioned biological particles. Furthermore, in some cases, it may be desirable to allow biological particles to incubate for a select period of time before analysis, such as in order to characterize changes in such biological particles over time, either in the presence or absence of different stimuli. In such cases, encapsulation may allow for longer incubation than partitioning in emulsion droplets, although in some cases, droplet partitioned biological particles may also be incubated for different periods of time, e.g., at least 10 seconds, at least 30 seconds, at least 1 minute, at least 5 minutes, at least 10 minutes, at least 30 minutes, at least 1 hour, at least 2 hours, at least 5 hours, or at least 10 hours or more. The encapsulation of biological particles may constitute the partitioning of the biological particles into which other reagents are co-partitioned. Alternatively or in addition, encapsulated biological particles may be readily deposited into other partitions (e.g., droplets) as described above.
Beads
A partition may comprise one or more unique identifiers, such as barcodes. Barcodes may be previously, subsequently or concurrently delivered to the partitions that hold the compartmentalized or partitioned biological particle. For example, barcodes may be injected into droplets previous to, subsequent to, or concurrently with droplet generation. The delivery of the barcodes to a particular partition allows for the later attribution of the characteristics of the individual biological particle to the particular partition. Barcodes may be delivered, for example on a nucleic acid molecule (e.g., an oligonucleotide), to a partition via any suitable mechanism. Barcoded nucleic acid molecules can be delivered to a partition via a microcapsule. A microcapsule, in some instances, can comprise a bead. Beads are described in further detail below.
In some cases, all or a portion of a collection of barcoded nucleic acid molecules can be initially associated with the microcapsule and then released from the microcapsule. Release of the barcoded nucleic acid molecules or sequences thereof can be passive (e.g., by diffusion out of the microcapsule). In addition or alternatively, release from the microcapsule can be upon application of a stimulus which allows the barcoded nucleic acid nucleic acid molecules or sequences thereof to dissociate or to be released from the microcapsule. Such stimulus may disrupt the microcapsule, an interaction that couples the barcoded nucleic acid molecules or sequences thereof to or within the microcapsule, or both. Such stimulus can include, for example, a thermal stimulus, photo-stimulus, chemical stimulus (e.g., change in pH or use of a reducing agent(s)), a mechanical stimulus, a radiation stimulus; a biological stimulus (e.g., enzyme), or any combination thereof.
As an alternative, the channel segments 201 and 202 may meet at another junction upstream of the junction 210. At such junction, beads and biological particles may form a mixture that is directed along another channel to the junction 210 to yield droplets 220. The mixture may provide the beads and biological particles in an alternating fashion, such that, for example, a droplet comprises a single bead and a single biological particle.
Beads, biological particles and droplets may flow along channels at substantially regular flow profiles (e.g., at regular flow rates). Such regular flow profiles may permit a droplet to include a single bead and a single biological particle. Such regular flow profiles may permit the droplets to have an occupancy (e.g., droplets having beads and biological particles) greater than 5%, 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, or 95%. Such regular flow profiles and devices that may be used to provide such regular flow profiles are provided in, for example, U.S. Patent Publication No. 2015/0292988, which is entirely incorporated herein by reference.
The second fluid 218 can comprise an oil, such as a fluorinated oil, that includes a fluorosurfactant for stabilizing the resulting droplets, for example, inhibiting subsequent coalescence of the resulting droplets 220.
A discrete droplet that is generated may include an individual biological particle 216. A discrete droplet that is generated may include a barcode or other reagent carrying bead 214. A discrete droplet generated may include both an individual biological particle and a barcode carrying bead, such as droplets 220. In some instances, a discrete droplet may include more than one individual biological particle or no biological particle. In some instances, a discrete droplet may include more than one bead or no bead. A discrete droplet may be unoccupied (e.g., no beads, no biological particles).
Beneficially, a discrete droplet partitioning a biological particle and a barcode carrying bead may effectively allow the attribution of the barcode to macromolecular constituents of the biological particle within the partition. The contents of a partition may remain discrete from the contents of other partitions.
As will be appreciated, the channel segments described herein may be coupled to any of a variety of different fluid sources or receiving components, including reservoirs, tubing, manifolds, or fluidic components of other systems. As will be appreciated, the microfluidic channel structure 200 may have other geometries. For example, a microfluidic channel structure can have more than one channel junctions. For example, a microfluidic channel structure can have 2, 3, 4, or 5 channel segments each carrying beads that meet at a channel junction. Fluid may be directed flow along one or more channels or reservoirs via one or more fluid flow units. A fluid flow unit can comprise compressors (e.g., providing positive pressure), pumps (e.g., providing negative pressure), actuators, and the like to control flow of the fluid. Fluid may also or otherwise be controlled via applied pressure differentials, centrifugal force, electrokinetic pumping, vacuum, capillary or gravity flow, or the like.
A bead may be porous, non-porous, solid, semi-solid, semi-fluidic, fluidic, and/or a combination thereof. In some instances, a bead may be dissolvable, disruptable, and/or degradable. In some cases, a bead may not be degradable. In some cases, the bead may be a gel bead. A gel bead may be a hydrogel bead. A gel bead may be formed from molecular precursors, such as a polymeric or monomeric species. A semi-solid bead may be a liposomal bead. Solid beads may comprise metals including iron oxide, gold, and silver. In some cases, the bead may be a silica bead. In some cases, the bead can be rigid. In other cases, the bead may be flexible and/or compressible.
A bead may be of any suitable shape. Examples of bead shapes include, but are not limited to, spherical, non-spherical, oval, oblong, amorphous, circular, cylindrical, and variations thereof.
Beads may be of uniform size or heterogeneous size. In some cases, the diameter of a bead may be at least about 10 nanometers (nm), 100 nm, 500 nm, 1 micrometer (μm), 5 μm, 10 μm, 20 μm, 30 μm, 40 μm, 50 μm, 60 μm, 70 μm, 80 μm, 90 μm, 100 μm, 250 μm, 500 μm, 1 mm, or greater. In some cases, a bead may have a diameter of less than about 10 nm, 100 nm, 500 nm, 1 μm, 5 μm, 10 μm, 20 μm, 30 μm, 40 μm, 50 μm, 60 μm, 70 μm, 80 μm, 90 μm, 100 μm, 250 μm, 500 μm, 1 mm, or less. In some cases, a bead may have a diameter in the range of about 40-75 μm, 30-75 μm, 20-75 μm, 40-85 μm, 40-95 μm, 20-100 μm, 10-100 μm, 1-100 μm, 20-250 μm, or 20-500 μm.
In certain aspects, beads can be provided as a population or plurality of beads having a relatively monodisperse size distribution. Where it may be desirable to provide relatively consistent amounts of reagents within partitions, maintaining relatively consistent bead characteristics, such as size, can contribute to the overall consistency. In particular, the beads described herein may have size distributions that have a coefficient of variation in their cross-sectional dimensions of less than 50%, less than 40%, less than 30%, less than 20%, and in some cases less than 15%, less than 10%, less than 5%, or less.
A bead may comprise natural and/or synthetic materials. For example, a bead can comprise a natural polymer, a synthetic polymer or both natural and synthetic polymers. Examples of natural polymers include proteins and sugars such as deoxyribonucleic acid, rubber, cellulose, starch (e.g., amylose, amylopectin), proteins, enzymes, polysaccharides, silks, polyhydroxyalkanoates, chitosan, dextran, collagen, carrageenan, ispaghula, acacia, agar, gelatin, shellac, sterculia gum, xanthan gum, Corn sugar gum, guar gum, gum karaya, agarose, alginic acid, alginate, or natural polymers thereof. Examples of synthetic polymers include acrylics, nylons, silicones, spandex, viscose rayon, polycarboxylic acids, polyvinyl acetate, polyacrylamide, polyacrylate, polyethylene glycol, polyurethanes, polylactic acid, silica, polystyrene, polyacrylonitrile, polybutadiene, polycarbonate, polyethylene, polyethylene terephthalate, poly(chlorotrifluoroethylene), poly(ethylene oxide), poly(ethylene terephthalate), polyethylene, polyisobutylene, poly(methyl methacrylate), poly(oxymethylene), polyformaldehyde, polypropylene, polystyrene, poly(tetrafluoroethylene), poly(vinyl acetate), poly(vinyl alcohol), poly(vinyl chloride), poly(vinylidene dichloride), poly(vinylidene difluoride), poly(vinyl fluoride) and/or combinations (e.g., co-polymers) thereof. Beads may also be formed from materials other than polymers, including lipids, micelles, ceramics, glass-ceramics, material composites, metals, other inorganic materials, and others.
In some instances, the bead may contain molecular precursors (e.g., monomers or polymers), which may form a polymer network via polymerization of the molecular precursors. In some cases, a precursor may be an already polymerized species capable of undergoing further polymerization via, for example, a chemical cross-linkage. In some cases, a precursor can comprise one or more of an acrylamide or a methacrylamide monomer, oligomer, or polymer. In some cases, the bead may comprise prepolymers, which are oligomers capable of further polymerization. For example, polyurethane beads may be prepared using prepolymers. In some cases, the bead may contain individual polymers that may be further polymerized together. In some cases, beads may be generated via polymerization of different precursors, such that they comprise mixed polymers, co-polymers, and/or block co-polymers. In some cases, the bead may comprise covalent or ionic bonds between polymeric precursors (e.g., monomers, oligomers, linear polymers), nucleic acid molecules (e.g., oligonucleotides), primers, and other entities. In some cases, the covalent bonds can be carbon-carbon bonds, thioether bonds, or carbon-heteroatom bonds.
Cross-linking may be permanent or reversible, depending upon the particular cross-linker used. Reversible cross-linking may allow for the polymer to linearize or dissociate under appropriate conditions. In some cases, reversible cross-linking may also allow for reversible attachment of a material bound to the surface of a bead. In some cases, a cross-linker may form disulfide linkages. In some cases, the chemical cross-linker forming disulfide linkages may be cystamine or a modified cystamine.
In some cases, disulfide linkages can be formed between molecular precursor units (e.g., monomers, oligomers, or linear polymers) or precursors incorporated into a bead and nucleic acid molecules (e.g., oligonucleotides). Cystamine (including modified cystamines), for example, is an organic agent comprising a disulfide bond that may be used as a crosslinker agent between individual monomeric or polymeric precursors of a bead. Polyacrylamide may be polymerized in the presence of cystamine or a species comprising cystamine (e.g., a modified cystamine) to generate polyacrylamide gel beads comprising disulfide linkages (e.g., chemically degradable beads comprising chemically-reducible cross-linkers). The disulfide linkages may permit the bead to be degraded (or dissolved) upon exposure of the bead to a reducing agent.
In some cases, chitosan, a linear polysaccharide polymer, may be crosslinked with glutaraldehyde via hydrophilic chains to form a bead. Crosslinking of chitosan polymers may be achieved by chemical reactions that are initiated by heat, pressure, change in pH, and/or radiation.
In some cases, a bead may comprise an acrydite moiety, which in certain aspects may be used to attach one or more nucleic acid molecules (e.g., barcode sequence, barcoded nucleic acid molecule, barcoded oligonucleotide, primer, or other oligonucleotide) to the bead. In some cases, an acrydite moiety can refer to an acrydite analogue generated from the reaction of acrydite with one or more species, such as, the reaction of acrydite with other monomers and cross-linkers during a polymerization reaction. Acrydite moieties may be modified to form chemical bonds with a species to be attached, such as a nucleic acid molecule (e.g., barcode sequence, barcoded nucleic acid molecule, barcoded oligonucleotide, primer, or other oligonucleotide). Acrydite moieties may be modified with thiol groups capable of forming a disulfide bond or may be modified with groups already comprising a disulfide bond. The thiol or disulfide (via disulfide exchange) may be used as an anchor point for a species to be attached or another part of the acrydite moiety may be used for attachment. In some cases, attachment can be reversible, such that when the disulfide bond is broken (e.g., in the presence of a reducing agent), the attached species is released from the bead. In other cases, an acrydite moiety can comprise a reactive hydroxyl group that may be used for attachment.
Functionalization of beads for attachment of nucleic acid molecules (e.g., oligonucleotides) may be achieved through a wide range of different approaches, including activation of chemical groups within a polymer, incorporation of active or activatable functional groups in the polymer structure, or attachment at the pre-polymer or monomer stage in bead production.
For example, precursors (e.g., monomers, cross-linkers) that are polymerized to form a bead may comprise acrydite moieties, such that when a bead is generated, the bead also comprises acrydite moieties. The acrydite moieties can be attached to a nucleic acid molecule (e.g., oligonucleotide), which may include a priming sequence (e.g., a primer for annealing to target nucleic acids, random primer, primer sequence for messenger RNA) and/or one or more barcode sequences. The one more barcode sequences may include sequences that are the same for all nucleic acid molecules coupled to a given bead and/or sequences that are different across all nucleic acid molecules coupled to the given bead. The nucleic acid molecule may be incorporated into the bead.
In some cases, the nucleic acid molecule can comprise a functional sequence, for example, for attachment to a sequencing flow cell, such as, for example, a P5 sequence for Illumina® sequencing. In some cases, the nucleic acid molecule or derivative thereof (e.g., oligonucleotide or polynucleotide generated from the nucleic acid molecule) can comprise another functional sequence, such as, for example, a P7 sequence for attachment to a sequencing flow cell for Illumina sequencing. In some cases, the nucleic acid molecule can comprise a barcode sequence. In some cases, the primer can further comprise a unique molecular identifier (UMI). In some cases, the primer can comprise an R1 primer sequence for Illumina sequencing. In some cases, the primer can comprise an R2 primer sequence for Illumina sequencing. Examples of such nucleic acid molecules (e.g., oligonucleotides, polynucleotides, etc.) and uses thereof, as may be used with compositions, devices, methods and systems of the present disclosure, are provided in U.S. Patent Pub. Nos. 2014/0378345 and 2015/0376609, each of which is entirely incorporated herein by reference.
The nucleic acid molecule 802 may comprise a unique molecular identifying sequence 816 (e.g., unique molecular identifier (UMI)). In some cases, the unique molecular identifying sequence 816 may comprise from about 5 to about 8 nucleotides. Alternatively, the unique molecular identifying sequence 816 may compress less than about 5 or more than about 8 nucleotides. The unique molecular identifying sequence 816 may be a unique sequence that varies across individual nucleic acid molecules (e.g., 802, 818, 820, etc.) coupled to a single bead (e.g., bead 804). In some cases, the unique molecular identifying sequence 816 may be a random sequence (e.g., such as a random N-mer sequence). For example, the UMI may provide a unique identifier of the starting mRNA molecule that was captured, in order to allow quantitation of the number of original expressed RNA. As will be appreciated, although
In operation, a biological particle (e.g., cell, DNA, RNA, etc.) can be co-partitioned along with a barcode bearing bead 804. The barcoded nucleic acid molecules 802, 818, 820, or sequences thereof, can be released from the bead 804 in the partition. By way of example, in the context of analyzing sample RNA, the poly-T segment (e.g., 812) of one of the released nucleic acid molecule sequences (e.g., 802) can hybridize to the poly-A tail of a mRNA molecule. Reverse transcription may result in a cDNA transcript of the mRNA, but which transcript includes each of the sequence segments 808, 810, 816 of the nucleic acid molecule 802. Because the nucleic acid molecule sequence 802 comprises an anchoring sequence 814, it will more likely hybridize to and prime reverse transcription at the sequence end of the poly-A tail of the mRNA. Within any given partition, all of the cDNA transcripts of the individual mRNA molecules may include a common barcode sequence segment 810. However, the transcripts made from the different mRNA molecules within a given partition may vary at the unique molecular identifying sequence 812 segment (e.g., UMI segment). Beneficially, even following any subsequent generation of one or more copies or complements of the contents of a given partition, the number of different UMIs can be indicative of the quantity of mRNA originating from a given partition, and thus from the biological particle (e.g., cell). As noted above, one or more copies or complements of the transcripts can be generated, cleaned up and sequenced to identify the sequence of the cDNA transcript of the mRNA, as well as to sequence the barcode segment and the UMI segment. While a poly-T primer sequence is described, other targeted or random priming sequences may also be used in priming the reverse transcription reaction. Likewise, although described as releasing the barcoded oligonucleotides or sequences thereof into the partition, in some cases, the nucleic acid molecules bound to the bead (e.g., gel bead) may be used to hybridize and capture the mRNA on the solid phase of the bead, for example, in order to facilitate the separation of the RNA from other cell contents.
In some cases, precursors comprising a functional group that is reactive or capable of being activated such that it becomes reactive can be polymerized with other precursors to generate gel beads comprising the activated or activatable functional group. The functional group may then be used to attach additional species (e.g., disulfide linkers, primers, other oligonucleotides, etc.) to the gel beads. For example, some precursors comprising a carboxylic acid (COOH) group can co-polymerize with other precursors to form a gel bead that also comprises a COOH functional group. In some cases, acrylic acid (a species comprising free COOH groups), acrylamide, and bis(acryloyl)cystamine can be co-polymerized together to generate a gel bead comprising free COOH groups. The COOH groups of the gel bead can be activated (e.g., via 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) and N-Hydroxysuccinimide (NHS) or 4-(4,6-Dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride (DMTMM)) such that they are reactive (e.g., reactive to amine functional groups where EDC/NHS or DMTMM are used for activation). The activated COOH groups can then react with an appropriate species (e.g., a species comprising an amine functional group where the carboxylic acid groups are activated to be reactive with an amine functional group) comprising a moiety to be linked to the bead.
Beads comprising disulfide linkages in their polymeric network may be functionalized with additional species via reduction of some of the disulfide linkages to free thiols. The disulfide linkages may be reduced via, for example, the action of a reducing agent (e.g., DTT, TCEP, etc.) to generate free thiol groups, without dissolution of the bead. Free thiols of the beads can then react with free thiols of a species or a species comprising another disulfide bond (e.g., via thiol-disulfide exchange) such that the species can be linked to the beads (e.g., via a generated disulfide bond). In some cases, free thiols of the beads may react with any other suitable group. For example, free thiols of the beads may react with species comprising an acrydite moiety. The free thiol groups of the beads can react with the acrydite via Michael addition chemistry, such that the species comprising the acrydite is linked to the bead. In some cases, uncontrolled reactions can be prevented by inclusion of a thiol capping agent such as N-ethylmalieamide or iodoacetate.
Activation of disulfide linkages within a bead can be controlled such that only a small number of disulfide linkages are activated. Control may be exerted, for example, by controlling the concentration of a reducing agent used to generate free thiol groups and/or concentration of reagents used to form disulfide bonds in bead polymerization. In some cases, a low concentration (e.g., molecules of reducing agent:gel bead ratios of less than or equal to about 1:100,000,000,000, less than or equal to about 1:10,000,000,000, less than or equal to about 1:1,000,000,000, less than or equal to about 1:100,000,000, less than or equal to about 1:10,000,000, less than or equal to about 1:1,000,000, less than or equal to about 1:100,000, less than or equal to about 1:10,000) of reducing agent may be used for reduction. Controlling the number of disulfide linkages that are reduced to free thiols may be useful in ensuring bead structural integrity during functionalization. In some cases, optically-active agents, such as fluorescent dyes may be coupled to beads via free thiol groups of the beads and used to quantify the number of free thiols present in a bead and/or track a bead.
In some cases, addition of moieties to a gel bead after gel bead formation may be advantageous. For example, addition of an oligonucleotide (e.g., barcoded oligonucleotide) after gel bead formation may avoid loss of the species during chain transfer termination that can occur during polymerization. Moreover, smaller precursors (e.g., monomers or cross linkers that do not comprise side chain groups and linked moieties) may be used for polymerization and can be minimally hindered from growing chain ends due to viscous effects. In some cases, functionalization after gel bead synthesis can minimize exposure of species (e.g., oligonucleotides) to be loaded with potentially damaging agents (e.g., free radicals) and/or chemical environments. In some cases, the generated gel may possess an upper critical solution temperature (UCST) that can permit temperature driven swelling and collapse of a bead. Such functionality may aid in oligonucleotide (e.g., a primer) infiltration into the bead during subsequent functionalization of the bead with the oligonucleotide. Post-production functionalization may also be useful in controlling loading ratios of species in beads, such that, for example, the variability in loading ratio is minimized. Species loading may also be performed in a batch process such that a plurality of beads can be functionalized with the species in a single batch.
A bead injected or otherwise introduced into a partition may comprise releasably, cleavably, or reversibly attached barcodes. For example, a bead may comprise oligonucleotides comprising releasable sequences comprising barcodes. A bead injected or otherwise introduced into a partition may comprise activatable barcodes. A bead injected or otherwise introduced into a partition may be degradable, disruptable, or dissolvable beads.
Barcodes can be releasably, cleavably or reversibly attached to the beads such that barcodes can be released or be releasable through cleavage of a linkage between the barcode molecule and the bead, or released through degradation of the underlying bead itself, allowing the barcodes to be accessed or be accessible by other reagents, or both. In non-limiting examples, cleavage may be achieved through reduction of di-sulfide bonds, use of restriction enzymes, photo-activated cleavage, or cleavage via other types of stimuli (e.g., chemical, thermal, pH, enzymatic, etc.) and/or reactions, such as described elsewhere herein. Releasable barcodes may sometimes be referred to as being activatable, in that they are available for reaction once released. Thus, for example, an activatable barcode may be activated by releasing the barcode from a bead (or other suitable type of partition described herein). Other activatable configurations are also envisioned in the context of the described methods and systems.
In addition to, or as an alternative to the cleavable linkages between the beads and the associated molecules, such as barcode containing nucleic acid molecules (e.g., barcoded oligonucleotides), the beads may be degradable, disruptable, or dissolvable spontaneously or upon exposure to one or more stimuli (e.g., temperature changes, pH changes, exposure to particular chemical species or phase, exposure to light, reducing agent, etc.). In some cases, a bead may be dissolvable, such that material components of the beads are solubilized when exposed to a particular chemical species or an environmental change, such as a change temperature or a change in pH. In some cases, a gel bead can be degraded or dissolved at elevated temperature and/or in basic conditions. In some cases, a bead may be thermally degradable such that when the bead is exposed to an appropriate change in temperature (e.g., heat), the bead degrades. Degradation or dissolution of a bead bound to a species (e.g., a nucleic acid molecule, e.g., barcoded oligonucleotide, or sequence thereof) may result in release of the species from the bead.
As will be appreciated from the above disclosure, the degradation of a bead may refer to the disassociation of a bound or entrained species from a bead, both with and without structurally degrading the physical bead itself. For example, the degradation of the bead may involve cleavage of a cleavable linkage via one or more species and/or methods described elsewhere herein. In another example, entrained species may be released from beads through osmotic pressure differences due to, for example, changing chemical environments. By way of example, alteration of bead pore sizes due to osmotic pressure differences can generally occur without structural degradation of the bead itself. In some cases, an increase in pore size due to osmotic swelling of a bead can permit the release of entrained species within the bead. In other cases, osmotic shrinking of a bead may cause a bead to better retain an entrained species due to pore size contraction.
A degradable bead may be introduced into a partition, such as a droplet of an emulsion or a well, such that the bead degrades within the partition and any associated species (e.g., oligonucleotides or sequences thereof) are released within the droplet when the appropriate stimulus is applied. The free species (e.g., oligonucleotides, nucleic acid molecules, or sequences thereof) may interact with other reagents contained in the partition. For example, a polyacrylamide bead comprising cystamine and linked, via a disulfide bond, to a barcode sequence, may be combined with a reducing agent within a droplet of a water-in-oil emulsion. Within the droplet, the reducing agent can break the various disulfide bonds, resulting in bead degradation and release of the barcode sequence into the aqueous, inner environment of the droplet. In another example, heating of a droplet comprising a bead-bound barcode sequence in basic solution may also result in bead degradation and release of the barcode sequence into the aqueous, inner environment of the droplet.
Any suitable number of molecular tag molecules (e.g., primer, barcoded oligonucleotide) can be associated with a bead such that, upon release from the bead, the molecular tag molecules (e.g., primer, e.g., barcoded oligonucleotide) or sequences thereof are present in the partition at a pre-defined concentration. Such pre-defined concentration may be selected to facilitate certain reactions for generating a sequencing library, e.g., primer extension reactions or amplification reactions, within the partition. In some cases, the pre-defined concentration of the primer can be limited by the process of producing nucleic acid molecule (e.g., oligonucleotide) bearing beads.
In some cases, beads can be non-covalently loaded with one or more reagents. The beads can be non-covalently loaded by, for instance, subjecting the beads to conditions sufficient to swell the beads, allowing sufficient time for the reagents to diffuse into the interiors of the beads, and subjecting the beads to conditions sufficient to de-swell the beads. The swelling of the beads may be accomplished, for instance, by placing the beads in a thermodynamically favorable solvent, subjecting the beads to a higher or lower temperature, subjecting the beads to a higher or lower ion concentration, and/or subjecting the beads to an electric field. The swelling of the beads may be accomplished by various swelling methods. The de-swelling of the beads may be accomplished, for instance, by transferring the beads in a thermodynamically unfavorable solvent, subjecting the beads to lower or high temperatures, subjecting the beads to a lower or higher ion concentration, and/or removing an electric field. The de-swelling of the beads may be accomplished by various de-swelling methods. Transferring the beads may cause pores in the bead to shrink. The shrinking may then hinder reagents within the beads from diffusing out of the interiors of the beads. The hindrance may be due to steric interactions between the reagents and the interiors of the beads. The transfer may be accomplished microfluidically. For instance, the transfer may be achieved by moving the beads from one co-flowing solvent stream to a different co-flowing solvent stream. The swellability and/or pore size of the beads may be adjusted by changing the polymer composition of the bead.
In some cases, an acrydite moiety linked to a precursor, another species linked to a precursor, or a precursor itself can comprise a labile bond, such as chemically, thermally, or photo-sensitive bond e.g., disulfide bond, UV sensitive bond, or the like. Once acrydite moieties or other moieties comprising a labile bond are incorporated into a bead, the bead may also comprise the labile bond. The labile bond may be, for example, useful in reversibly linking (e.g., covalently linking) species (e.g., barcodes, primers, etc.) to a bead. In some cases, a thermally labile bond may include a nucleic acid hybridization based attachment, e.g., where an oligonucleotide is hybridized to a complementary sequence that is attached to the bead, such that thermal melting of the hybrid releases the sequence of the oligonucleotide, e.g., a barcode containing sequence, from the bead or microcapsule.
The addition of multiple types of labile bonds to a gel bead may result in the generation of a bead capable of responding to varied stimuli. Each type of labile bond may be sensitive to an associated stimulus (e.g., chemical stimulus, light, temperature, enzymatic, etc.) such that release of species attached to a bead via each labile bond may be controlled by the application of the appropriate stimulus. Such functionality may be useful in controlled release of species from a gel bead. In some cases, another species comprising a labile bond may be linked to a gel bead after gel bead formation via, for example, an activated functional group of the gel bead as described above. As will be appreciated, barcode sequences that are releasably, cleavably or reversibly attached to the beads described herein (e.g., as components of sequences of nucleic acid barcode molecules or oligonucleotides) include barcode sequences that are released or releasable through cleavage of a linkage between the barcode molecule and the bead, or that are released through degradation of the underlying bead itself, allowing the barcodes to be accessed or accessible by other reagents, or both.
The barcode sequences that are releasable as described herein may sometimes be referred to as being activatable, in that they are available for reaction once released. Thus, for example, an activatable barcode may be activated by releasing the barcode sequence from a bead (or other suitable type of partition described herein). Other activatable configurations are also envisioned in the context of the described methods and systems.
In addition to thermally cleavable bonds, disulfide bonds and UV sensitive bonds, other non-limiting examples of labile bonds that may be coupled to a precursor or bead include an ester linkage (e.g., cleavable with an acid, a base, or hydroxylamine), a vicinal diol linkage (e.g., cleavable via sodium periodate), a Diels-Alder linkage (e.g., cleavable via heat), a sulfone linkage (e.g., cleavable via a base), a silyl ether linkage (e.g., cleavable via an acid), a glycosidic linkage (e.g., cleavable via an amylase), a peptide linkage (e.g., cleavable via a protease), or a phosphodiester linkage (e.g., cleavable via a nuclease (e.g., DNAase)). A bond may be cleavable via other nucleic acid molecule targeting enzymes, such as restriction enzymes (e.g., restriction endonucleases), as described further below.
Species may be encapsulated in beads during bead generation (e.g., during polymerization of precursors). Such species may or may not participate in polymerization. Such species may be entered into polymerization reaction mixtures such that generated beads comprise the species upon bead formation. In some cases, such species may be added to the gel beads after formation. Such species may include, for example, nucleic acid molecules (e.g., oligonucleotides), reagents for a nucleic acid extension and/or amplification reaction (e.g., primers, polymerases, dNTPs, co-factors (e.g., ionic co-factors), buffers) including those described herein, reagents for enzymatic reactions (e.g., enzymes, co-factors, substrates, buffers), reagents for nucleic acid modification reactions such as polymerization, ligation, or digestion, and/or reagents for template preparation (e.g., tagmentation) for one or more sequencing platforms (e.g., Nextera® for Illumina®). Such species may include one or more enzymes described herein, including without limitation, polymerase, reverse transcriptase, restriction enzymes (e.g., endonuclease), transposase, ligase, proteinase K, DNAse, etc. Such species may include one or more reagents described elsewhere herein (e.g., lysis agents, inhibitors, inactivating agents, chelating agents, stimulus). Trapping of such species may be controlled by the polymer network density generated during polymerization of precursors, control of ionic charge within the gel bead (e.g., via ionic species linked to polymerized species), or by the release of other species. Encapsulated species may be released from a bead upon bead degradation and/or by application of a stimulus capable of releasing the species from the bead. Alternatively or in addition, species may be partitioned in a partition (e.g., droplet) during or subsequent to partition formation. Such species may include, without limitation, the abovementioned species that may also be encapsulated in a bead.
A degradable bead may comprise one or more species with a labile bond such that, when the bead/species is exposed to the appropriate stimuli, the bond is broken and the bead degrades. The labile bond may be a chemical bond (e.g., covalent bond, ionic bond) or may be another type of physical interaction (e.g., van der Waals interactions, dipole-dipole interactions, etc.). In some cases, a crosslinker used to generate a bead may comprise a labile bond. Upon exposure to the appropriate conditions, the labile bond can be broken and the bead degraded. For example, upon exposure of a polyacrylamide gel bead comprising cystamine crosslinkers to a reducing agent, the disulfide bonds of the cystamine can be broken and the bead degraded.
A degradable bead may be useful in more quickly releasing an attached species (e.g., a nucleic acid molecule, a barcode sequence, a primer, etc.) or component thereof from the bead when the appropriate stimulus is applied to the bead as compared to a bead that does not degrade. For example, for a species bound to an inner surface of a porous bead or in the case of an encapsulated species, the species may have greater mobility and accessibility to other species in solution upon degradation of the bead. In some cases, a species may also be attached to a degradable bead via a degradable linker (e.g., disulfide linker). The degradable linker may respond to the same stimuli as the degradable bead or the two degradable species may respond to different stimuli. For example, a barcode sequence may be attached, via a disulfide bond, to a polyacrylamide bead comprising cystamine. Upon exposure of the barcoded-bead to a reducing agent, the bead degrades and the barcode sequence is released upon breakage of both the disulfide linkage between the barcode sequence and the bead and the disulfide linkages of the cystamine in the bead.
As will be appreciated from the above disclosure, while referred to as degradation of a bead, in many instances as noted above, that degradation may refer to the disassociation of a bound or entrained species from a bead, both with and without structurally degrading the physical bead itself. For example, entrained species may be released from beads through osmotic pressure differences due to, for example, changing chemical environments. By way of example, alteration of bead pore sizes due to osmotic pressure differences can generally occur without structural degradation of the bead itself. In some cases, an increase in pore size due to osmotic swelling of a bead can permit the release of entrained species within the bead. In other cases, osmotic shrinking of a bead may cause a bead to better retain an entrained species due to pore size contraction.
Where degradable beads are provided, it may be beneficial to avoid exposing such beads to the stimulus or stimuli that cause such degradation prior to a given time, in order to, for example, avoid premature bead degradation and issues that arise from such degradation, including for example poor flow characteristics and aggregation. By way of example, where beads comprise reducible cross-linking groups, such as disulfide groups, it will be desirable to avoid contacting such beads with reducing agents, e.g., DTT or other disulfide cleaving reagents. In such cases, treatment to the beads described herein will, in some cases be provided free of reducing agents, such as DTT. Because reducing agents are often provided in commercial enzyme preparations, it may be desirable to provide reducing agent free (or DTT free) enzyme preparations in treating the beads described herein. Examples of such enzymes include, e.g., polymerase enzyme preparations, reverse transcriptase enzyme preparations, ligase enzyme preparations, as well as many other enzyme preparations that may be used to treat the beads described herein. The terms “reducing agent free” or “DTT free” preparations can refer to a preparation having less than about 1/10th, less than about 1/50th, or even less than about 1/100th of the lower ranges for such materials used in degrading the beads. For example, for DTT, the reducing agent free preparation can have less than about 0.01 millimolar (mM), 0.005 mM, 0.001 mM DTT, 0.0005 mM DTT, or even less than about 0.0001 mM DTT. In many cases, the amount of DTT can be undetectable.
Numerous chemical triggers may be used to trigger the degradation of beads. Examples of these chemical changes may include, but are not limited to pH-mediated changes to the integrity of a component within the bead, degradation of a component of a bead via cleavage of cross-linked bonds, and depolymerization of a component of a bead.
In some embodiments, a bead may be formed from materials that comprise degradable chemical crosslinkers, such as BAC or cystamine. Degradation of such degradable crosslinkers may be accomplished through a number of mechanisms. In some examples, a bead may be contacted with a chemical degrading agent that may induce oxidation, reduction or other chemical changes. For example, a chemical degrading agent may be a reducing agent, such as dithiothreitol (DTT). Additional examples of reducing agents may include β-mercaptoethanol, (2S)-2-amino-1,4-dimercaptobutane (dithiobutylamine or DTBA), tris(2-carboxyethyl) phosphine (TCEP), or combinations thereof. A reducing agent may degrade the disulfide bonds formed between gel precursors forming the bead, and thus, degrade the bead. In other cases, a change in pH of a solution, such as an increase in pH, may trigger degradation of a bead. In other cases, exposure to an aqueous solution, such as water, may trigger hydrolytic degradation, and thus degradation of the bead. In some cases, any combination of stimuli may trigger degradation of a bead. For example, a change in pH may enable a chemical agent (e.g., DTT) to become an effective reducing agent.
Beads may also be induced to release their contents upon the application of a thermal stimulus. A change in temperature can cause a variety of changes to a bead. For example, heat can cause a solid bead to liquefy. A change in heat may cause melting of a bead such that a portion of the bead degrades. In other cases, heat may increase the internal pressure of the bead components such that the bead ruptures or explodes. Heat may also act upon heat-sensitive polymers used as materials to construct beads.
Any suitable agent may degrade beads. In some embodiments, changes in temperature or pH may be used to degrade thermo-sensitive or pH-sensitive bonds within beads. In some embodiments, chemical degrading agents may be used to degrade chemical bonds within beads by oxidation, reduction or other chemical changes. For example, a chemical degrading agent may be a reducing agent, such as DTT, wherein DTT may degrade the disulfide bonds formed between a crosslinker and gel precursors, thus degrading the bead. In some embodiments, a reducing agent may be added to degrade the bead, which may or may not cause the bead to release its contents. Examples of reducing agents may include dithiothreitol (DTT), β-mercaptoethanol, (2S)-2-amino-1,4-dimercaptobutane (dithiobutylamine or DTBA), tris(2-carboxyethyl) phosphine (TCEP), or combinations thereof. The reducing agent may be present at a concentration of about 0.1 mM, 0.5 mM, 1 mM, 5 mM, 10 mM. The reducing agent may be present at a concentration of at least about 0.1 mM, 0.5 mM, 1 mM, 5 mM, 10 mM, or greater than 10 mM. The reducing agent may be present at concentration of at most about 10 mM, 5 mM, 1 mM, 0.5 mM, 0.1 mM, or less.
Any suitable number of molecular tag molecules (e.g., primer, barcoded oligonucleotide) can be associated with a bead such that, upon release of the molecule tag molecules or components thereof (e.g., sequences thereof, such as sequences comprising barcode sequences) from the bead, the molecular tag molecules (e.g., primer, e.g., barcoded oligonucleotide) or components thereof are present in the partition at a pre-defined concentration. Such pre-defined concentration may be selected to facilitate certain reactions for generating a sequencing library, e.g., extension reactions or amplification reactions, within the partition. In some cases, the pre-defined concentration of the primer can be limited by the process of producing oligonucleotide bearing beads.
Although
In some cases, additional microcapsules can be used to deliver additional reagents to a partition. In such cases, it may be advantageous to introduce different beads into a common channel or droplet generation junction, from different bead sources (e.g., containing different associated reagents) through different channel inlets into such common channel or droplet generation junction (e.g., junction 210). In such cases, the flow and frequency of the different beads into the channel or junction may be controlled to provide for a certain ratio of microcapsules from each source, while ensuring a given pairing or combination of such beads into a partition with a given number of biological particles (e.g., one biological particle and one bead per partition).
The partitions described herein may comprise small volumes, for example, less than about 10 microliters (μL), 5 μL, 1 μL, 900 picoliters (pL), 800 pL, 700 pL, 600 pL, 500 pL, 400 pL, 300 pL, 200 pL, 100 pL, 50 pL, 20 pL, 10 pL, 1 pL, 500 nanoliters (nL), 100 nL, 50 nL, or less.
For example, in the case of droplet based partitions, the droplets may have overall volumes that are less than about 1000 pL, 900 pL, 800 pL, 700 pL, 600 pL, 500 pL, 400 pL, 300 pL, 200 pL, 100 pL, 50 pL, 20 pL, 10 pL, 1 pL, or less. Where co-partitioned with microcapsules, it will be appreciated that the sample fluid volume, e.g., including co-partitioned biological particles and/or beads, within the partitions may be less than about 90% of the above described volumes, less than about 80%, less than about 70%, less than about 60%, less than about 50%, less than about 40%, less than about 30%, less than about 20%, or less than about 10% of the above described volumes.
As is described elsewhere herein, partitioning species may generate a population or plurality of partitions. In such cases, any suitable number of partitions can be generated or otherwise provided. For example, at least about 1,000 partitions, at least about 5,000 partitions, at least about 10,000 partitions, at least about 50,000 partitions, at least about 100,000 partitions, at least about 500,000 partitions, at least about 1,000,000 partitions, at least about 5,000,000 partitions at least about 10,000,000 partitions, at least about 50,000,000 partitions, at least about 100,000,000 partitions, at least about 500,000,000 partitions, at least about 1,000,000,000 partitions, or more partitions can be generated or otherwise provided. Moreover, the plurality of partitions may comprise both unoccupied partitions (e.g., empty partitions) and occupied partitions.
Reagents
In accordance with certain aspects, biological particles may be partitioned along with lysis reagents in order to release the contents of the biological particles within the partition. In such cases, the lysis agents can be contacted with the biological particle suspension concurrently with, or immediately prior to, the introduction of the biological particles into the partitioning junction/droplet generation zone (e.g., junction 210), such as through an additional channel or channels upstream of the channel junction. In accordance with other aspects, additionally or alternatively, biological particles may be partitioned along with other reagents, as will be described further below.
In an example operation, the channel segment 301 may transport an aqueous fluid 312 that includes a plurality of biological particles 314 along the channel segment 301 into the second junction 310. As an alternative or in addition to, channel segment 301 may transport beads (e.g., gel beads). The beads may comprise barcode molecules.
For example, the channel segment 301 may be connected to a reservoir comprising an aqueous suspension of biological particles 314. Upstream of, and immediately prior to reaching, the second junction 310, the channel segment 301 may meet the channel segment 302 at the first junction 309. The channel segment 302 may transport a plurality of reagents 315 (e.g., lysis agents) suspended in the aqueous fluid 312 along the channel segment 302 into the first junction 309. For example, the channel segment 302 may be connected to a reservoir comprising the reagents 315. After the first junction 309, the aqueous fluid 312 in the channel segment 301 can carry both the biological particles 314 and the reagents 315 towards the second junction 310. In some instances, the aqueous fluid 312 in the channel segment 301 can include one or more reagents, which can be the same or different reagents as the reagents 315. A second fluid 316 that is immiscible with the aqueous fluid 312 (e.g., oil) can be delivered to the second junction 310 from each of channel segments 304 and 306. Upon meeting of the aqueous fluid 312 from the channel segment 301 and the second fluid 316 from each of channel segments 304 and 306 at the second channel junction 310, the aqueous fluid 312 can be partitioned as discrete droplets 318 in the second fluid 316 and flow away from the second junction 310 along channel segment 308. The channel segment 308 may deliver the discrete droplets 318 to an outlet reservoir fluidly coupled to the channel segment 308, where they may be harvested.
The second fluid 316 can comprise an oil, such as a fluorinated oil, that includes a fluorosurfactant for stabilizing the resulting droplets, for example, inhibiting subsequent coalescence of the resulting droplets 318.
A discrete droplet generated may include an individual biological particle 314 and/or one or more reagents 315. In some instances, a discrete droplet generated may include a barcode carrying bead (not shown), such as via other microfluidics structures described elsewhere herein. In some instances, a discrete droplet may be unoccupied (e.g., no reagents, no biological particles).
Beneficially, when lysis reagents and biological particles are co-partitioned, the lysis reagents can facilitate the release of the contents of the biological particles within the partition. The contents released in a partition may remain discrete from the contents of other partitions.
As will be appreciated, the channel segments described herein may be coupled to any of a variety of different fluid sources or receiving components, including reservoirs, tubing, manifolds, or fluidic components of other systems. As will be appreciated, the microfluidic channel structure 300 may have other geometries. For example, a microfluidic channel structure can have more than two channel junctions. For example, a microfluidic channel structure can have 2, 3, 4, 5 channel segments or more each carrying the same or different types of beads, reagents, and/or biological particles that meet at a channel junction. Fluid flow in each channel segment may be controlled to control the partitioning of the different elements into droplets. Fluid may be directed flow along one or more channels or reservoirs via one or more fluid flow units. A fluid flow unit can comprise compressors (e.g., providing positive pressure), pumps (e.g., providing negative pressure), actuators, and the like to control flow of the fluid. Fluid may also or otherwise be controlled via applied pressure differentials, centrifugal force, electrokinetic pumping, vacuum, capillary or gravity flow, or the like.
Examples of lysis agents include bioactive reagents, such as lysis enzymes that are used for lysis of different cell types, e.g., gram positive or negative bacteria, plants, yeast, mammalian, etc., such as lysozymes, achromopeptidase, lysostaphin, labiase, kitalase, lyticase, and a variety of other lysis enzymes available from, e.g., Sigma-Aldrich, Inc. (St Louis, MO), as well as other commercially available lysis enzymes. Other lysis agents may additionally or alternatively be co-partitioned with the biological particles to cause the release of the biological particles' contents into the partitions. For example, in some cases, surfactant-based lysis solutions may be used to lyse cells, although these may be less desirable for emulsion based systems where the surfactants can interfere with stable emulsions. In some cases, lysis solutions may include non-ionic surfactants such as, for example, TritonX-100 and Tween 20. In some cases, lysis solutions may include ionic surfactants such as, for example, sarcosyl and sodium dodecyl sulfate (SDS). Electroporation, thermal, acoustic or mechanical cellular disruption may also be used in certain cases, e.g., non-emulsion based partitioning such as encapsulation of biological particles that may be in addition to or in place of droplet partitioning, where any pore size of the encapsulate is sufficiently small to retain nucleic acid fragments of a given size, following cellular disruption.
Alternatively or in addition to the lysis agents co-partitioned with the biological particles described above, other reagents can also be co-partitioned with the biological particles, including, for example, DNase and RNase inactivating agents or inhibitors, such as proteinase K, chelating agents, such as EDTA, and other reagents employed in removing or otherwise reducing negative activity or impact of different cell lysate components on subsequent processing of nucleic acids. In addition, in the case of encapsulated biological particles, the biological particles may be exposed to an appropriate stimulus to release the biological particles or their contents from a co-partitioned microcapsule. For example, in some cases, a chemical stimulus may be co-partitioned along with an encapsulated biological particle to allow for the degradation of the microcapsule and release of the cell or its contents into the larger partition. In some cases, this stimulus may be the same as the stimulus described elsewhere herein for release of nucleic acid molecules (e.g., oligonucleotides) or sequences thereof from their respective microcapsule (e.g., bead). In alternative aspects, this may be a different and non-overlapping stimulus, in order to allow an encapsulated biological particle to be released into a partition at a different time from the release of nucleic acid molecules or sequences thereof into the same partition.
Additional reagents may also be co-partitioned with the biological particles, such as endonucleases to fragment a biological particle's DNA, DNA polymerase enzymes and dNTPs used to generate copies or complements of the biological particle's nucleic acid fragments and to attach the barcode molecular tags to the copies or complements. Other enzymes may be co-partitioned, including without limitation, polymerase, transposase, ligase, proteinase K, DNAse, etc. Additional reagents may also include reverse transcriptase enzymes, including enzymes with terminal transferase activity, primers and oligonucleotides, and switch oligonucleotides (also referred to herein as “switch oligos” or “template switching oligonucleotides”) which can be used for template switching. In some cases, template switching can be used to increase the length of a cDNA. In some cases, template switching can be used to append a predefined nucleic acid sequence to the cDNA. In an example of template switching, cDNA can be generated from reverse transcription of a template, e.g., cellular mRNA, where a reverse transcriptase with terminal transferase activity can add additional nucleotides, e.g., polyC, to the cDNA in a template independent manner. Switch oligos can include sequences complementary to the additional nucleotides, e.g., polyG. The additional nucleotides (e.g., polyC) on the cDNA can hybridize to the additional nucleotides (e.g., polyG) on the switch oligo, whereby the switch oligo can be used by the reverse transcriptase as template to further extend the cDNA. Template switching oligonucleotides may comprise a hybridization region and a template region. The hybridization region can comprise any sequence capable of hybridizing to the target. In some cases, as previously described, the hybridization region comprises a series of G bases to complement the overhanging C bases at the 3′ end of a cDNA molecule. The series of G bases may comprise 1 G base, 2 G bases, 3 G bases, 4 G bases, 5 G bases or more than 5 G bases. The template sequence can comprise any sequence to be incorporated into the cDNA. In some cases, the template region comprises at least 1 (e.g., at least 2, 3, 4, 5 or more) tag sequences and/or functional sequences. Switch oligos may comprise deoxyribonucleic acids; ribonucleic acids; modified nucleic acids including 2-Aminopurine, 2,6-Diaminopurine (2-Amino-dA), inverted dT, 5-Methyl dC, 2′-deoxyInosine, Super T (5-hydroxybutynl-2′-deoxyuridine), Super G (8-aza-7-deazaguanosine), locked nucleic acids (LNAs), unlocked nucleic acids (UNAs, e.g., UNA-A, UNA-U, UNA-C, UNA-G), Iso-dG, Iso-dC, 2′ Fluoro bases (e.g., Fluoro C, Fluoro U, Fluoro A, and Fluoro G), or any combination.
In some cases, the length of a switch oligo may be at least about 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126, 127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140, 141, 142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 161, 162, 163, 164, 165, 166, 167, 168, 169, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 184, 185, 186, 187, 188, 189, 190, 191, 192, 193, 194, 195, 196, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 219, 220, 221, 222, 223, 224, 225, 226, 227, 228, 229, 230, 231, 232, 233, 234, 235, 236, 237, 238, 239, 240, 241, 242, 243, 244, 245, 246, 247, 248, 249 or 250 nucleotides or longer.
In some cases, the length of a switch oligo may be at most about 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126, 127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140, 141, 142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 161, 162, 163, 164, 165, 166, 167, 168, 169, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 184, 185, 186, 187, 188, 189, 190, 191, 192, 193, 194, 195, 196, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 219, 220, 221, 222, 223, 224, 225, 226, 227, 228, 229, 230, 231, 232, 233, 234, 235, 236, 237, 238, 239, 240, 241, 242, 243, 244, 245, 246, 247, 248, 249 or 250 nucleotides.
Once the contents of the cells are released into their respective partitions, the macromolecular components (e.g., macromolecular constituents of biological particles, such as RNA, DNA, or proteins) contained therein may be further processed within the partitions. In accordance with the methods and systems described herein, the macromolecular component contents of individual biological particles can be provided with unique identifiers such that, upon characterization of those macromolecular components they may be attributed as having been derived from the same biological particle or particles. The ability to attribute characteristics to individual biological particles or groups of biological particles is provided by the assignment of unique identifiers specifically to an individual biological particle or groups of biological particles. Unique identifiers, e.g., in the form of nucleic acid barcodes can be assigned or associated with individual biological particles or populations of biological particles, in order to tag or label the biological particle's macromolecular components (and as a result, its characteristics) with the unique identifiers. These unique identifiers can then be used to attribute the biological particle's components and characteristics to an individual biological particle or group of biological particles.
In some aspects, this is performed by co-partitioning the individual biological particle or groups of biological particles with the unique identifiers, such as described above (with reference to
The nucleic acid barcode sequences can include from about 6 to about 20 or more nucleotides within the sequence of the nucleic acid molecules (e.g., oligonucleotides). The nucleic acid barcode sequences can include from about 6 to about 20, 30, 40, 50, 60, 70, 80, 90, 100 or more nucleotides. In some cases, the length of a barcode sequence may be about 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20 nucleotides or longer. In some cases, the length of a barcode sequence may be at least about 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20 nucleotides or longer. In some cases, the length of a barcode sequence may be at most about 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20 nucleotides or shorter. These nucleotides may be completely contiguous, i.e., in a single stretch of adjacent nucleotides, or they may be separated into two or more separate subsequences that are separated by 1 or more nucleotides. In some cases, separated barcode subsequences can be from about 4 to about 16 nucleotides in length. In some cases, the barcode subsequence may be about 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16 nucleotides or longer. In some cases, the barcode subsequence may be at least about 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16 nucleotides or longer. In some cases, the barcode subsequence may be at most about 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16 nucleotides or shorter.
The co-partitioned nucleic acid molecules can also comprise other functional sequences useful in the processing of the nucleic acids from the co-partitioned biological particles. These sequences include, e.g., targeted or random/universal primer sequences (e.g., universal amplification primer sequences) for generating copies and/or complements of (e.g., amplifying) the genomic DNA from the individual biological particles within the partitions while attaching the associated barcode sequences, sequencing primers or primer recognition sites, hybridization or probing sequences, e.g., for identification of presence of the sequences or for pulling down barcoded nucleic acids, or any of a number of other potential functional sequences. Other mechanisms of co-partitioning oligonucleotides may also be employed, including, e.g., coalescence of two or more droplets, where one droplet contains oligonucleotides, or microdispensing of oligonucleotides into partitions, e.g., droplets within microfluidic systems.
In an example, microcapsules, such as beads, are provided that each include large numbers of the above described barcoded nucleic acid molecules (e.g., barcoded oligonucleotides) attached to the beads, where all of the nucleic acid molecules attached to a particular bead will include the same nucleic acid barcode sequence, but where a large number of diverse barcode sequences are represented across the population of beads used. In some embodiments, hydrogel beads, e.g., comprising polyacrylamide polymer matrices, are used as a solid support and delivery vehicle for the nucleic acid molecules into the partitions, as they are capable of carrying large numbers of nucleic acid molecules, and may be configured to release those nucleic acid molecules or sequences thereof upon exposure to a particular stimulus, as described elsewhere herein. In some cases, the population of beads provides a diverse barcode sequence library that includes at least about 1,000 different barcode sequences, at least about 5,000 different barcode sequences, at least about 10,000 different barcode sequences, at least about 50,000 different barcode sequences, at least about 100,000 different barcode sequences, at least about 1,000,000 different barcode sequences, at least about 5,000,000 different barcode sequences, or at least about 10,000,000 different barcode sequences, or more. Additionally, each bead can be provided with large numbers of nucleic acid (e.g., oligonucleotide) molecules attached. In particular, the number of molecules of nucleic acid molecules including the barcode sequence on an individual bead can be at least about 1,000 nucleic acid molecules, at least about 5,000 nucleic acid molecules, at least about 10,000 nucleic acid molecules, at least about 50,000 nucleic acid molecules, at least about 100,000 nucleic acid molecules, at least about 500,000 nucleic acids, at least about 1,000,000 nucleic acid molecules, at least about 5,000,000 nucleic acid molecules, at least about 10,000,000 nucleic acid molecules, at least about 50,000,000 nucleic acid molecules, at least about 100,000,000 nucleic acid molecules, at least about 250,000,000 nucleic acid molecules and in some cases at least about 1 billion nucleic acid molecules, or more. Nucleic acid molecules of a given bead can include identical (or common) barcode sequences, different barcode sequences, or a combination of both. Nucleic acid molecules of a given bead can include multiple sets of nucleic acid molecules. Nucleic acid molecules of a given set can include identical barcode sequences. The identical barcode sequences can be different from barcode sequences of nucleic acid molecules of another set.
Moreover, when the population of beads is partitioned, the resulting population of partitions can also include a diverse barcode library that includes at least about 1,000 different barcode sequences, at least about 5,000 different barcode sequences, at least about 10,000 different barcode sequences, at least at least about 50,000 different barcode sequences, at least about 100,000 different barcode sequences, at least about 1,000,000 different barcode sequences, at least about 5,000,000 different barcode sequences, or at least about 10,000,000 different barcode sequences. Additionally, each partition of the population can include at least about 1,000 nucleic acid molecules, at least about 5,000 nucleic acid molecules, at least about 10,000 nucleic acid molecules, at least about 50,000 nucleic acid molecules, at least about 100,000 nucleic acid molecules, at least about 500,000 nucleic acids, at least about 1,000,000 nucleic acid molecules, at least about 5,000,000 nucleic acid molecules, at least about 10,000,000 nucleic acid molecules, at least about 50,000,000 nucleic acid molecules, at least about 100,000,000 nucleic acid molecules, at least about 250,000,000 nucleic acid molecules and in some cases at least about 1 billion nucleic acid molecules.
In some cases, it may be desirable to incorporate multiple different barcodes within a given partition, either attached to a single or multiple beads within the partition. For example, in some cases, a mixed, but known set of barcode sequences may provide greater assurance of identification in the subsequent processing, e.g., by providing a stronger address or attribution of the barcodes to a given partition, as a duplicate or independent confirmation of the output from a given partition.
The nucleic acid molecules (e.g., oligonucleotides) or sequences thereof are releasable from the beads upon the application of a particular stimulus to the beads. In some cases, the stimulus may be a photo-stimulus, e.g., through cleavage of a photo-labile linkage that releases the sequences of the nucleic acid molecules. In other cases, a thermal stimulus may be used, where elevation of the temperature of the beads environment will result in cleavage of a linkage or other release of the sequences of the nucleic acid molecules form the beads. In still other cases, a chemical stimulus can be used that cleaves a linkage of the nucleic acid molecules to the beads, or otherwise results in release of the sequences of the nucleic acid molecules from the beads. In one case, such compositions include the polyacrylamide matrices described above for encapsulation of biological particles, and may be degraded for release of the attached nucleic acid molecules or sequences thereof through exposure to a reducing agent, such as DTT.
In some aspects, provided are systems and methods for controlled partitioning. Droplet size may be controlled by adjusting certain geometric features in channel architecture (e.g., microfluidics channel architecture). For example, an expansion angle, width, and/or length of a channel may be adjusted to control droplet size.
A discrete droplet generated may include a bead (e.g., as in occupied droplets 416). Alternatively, a discrete droplet generated may include more than one bead. Alternatively, a discrete droplet generated may not include any beads (e.g., as in unoccupied droplet 418). In some instances, a discrete droplet generated may contain one or more biological particles, as described elsewhere herein. In some instances, a discrete droplet generated may comprise one or more reagents, as described elsewhere herein.
In some instances, the aqueous fluid 408 can have a substantially uniform concentration or frequency of beads 412. The beads 412 can be introduced into the channel segment 402 from a separate channel (not shown in
In some instances, the aqueous fluid 408 in the channel segment 402 can comprise biological particles (e.g., described with reference to
The second fluid 410 can comprise an oil, such as a fluorinated oil, that includes a fluorosurfactant for stabilizing the resulting droplets, for example, inhibiting subsequent coalescence of the resulting droplets.
In some instances, the second fluid 410 may not be subjected to and/or directed to any flow in or out of the reservoir 404. For example, the second fluid 410 may be substantially stationary in the reservoir 404. In some instances, the second fluid 410 may be subjected to flow within the reservoir 404, but not in or out of the reservoir 404, such as via application of pressure to the reservoir 404 and/or as affected by the incoming flow of the aqueous fluid 408 at the junction 406. Alternatively, the second fluid 410 may be subjected and/or directed to flow in or out of the reservoir 404. For example, the reservoir 404 can be a channel directing the second fluid 410 from upstream to downstream, transporting the generated droplets.
The channel structure 400 at or near the junction 406 may have certain geometric features that at least partly determine the sizes of the droplets formed by the channel structure 400. The channel segment 402 can have a height, h0 and width, w, at or near the junction 406. By way of example, the channel segment 402 can comprise a rectangular cross-section that leads to a reservoir 404 having a wider cross-section (such as in width or diameter). Alternatively, the cross-section of the channel segment 402 can be other shapes, such as a circular shape, trapezoidal shape, polygonal shape, or any other shapes. The top and bottom walls of the reservoir 404 at or near the junction 406 can be inclined at an expansion angle, α. The expansion angle, α, allows the tongue (portion of the aqueous fluid 408 leaving channel segment 402 at junction 406 and entering the reservoir 404 before droplet formation) to increase in depth and facilitate decrease in curvature of the intermediately formed droplet. Droplet size may decrease with increasing expansion angle. The resulting droplet radius, Rd, may be predicted by the following equation for the aforementioned geometric parameters of h0, w, and α:
By way of example, for a channel structure with w=21 μm, h=21 μm, and α=3°, the predicted droplet size is 121 μm. In another example, for a channel structure with w=25 μm, h=25 μm, and α=5°, the predicted droplet size is 123 μm. In another example, for a channel structure with w=28 μm, h=28 μm, and α=7°, the predicted droplet size is 124 μm.
In some instances, the expansion angle, α, may be between a range of from about 0.5° to about 4°, from about 0.1° to about 10°, or from about 0° to about 90°. For example, the expansion angle can be at least about 0.01°, 0.1°, 0.2°, 0.3°, 0.4°, 0.5°, 0.6°, 0.7°, 0.8°, 0.9°, 1°, 2°, 3°, 4°, 5°, 6°, 7°, 8°, 9°, 10°, 15°, 20°, 25°, 30°, 35°, 40°, 45°, 50°, 55°, 60°, 65°, 70°, 75°, 80°, 85°, or higher. In some instances, the expansion angle can be at most about 89°, 88°, 87°, 86°, 85°, 84°, 83°, 82°, 81°, 80°, 75°, 70°, 65°, 60°, 55°, 50°, 45°, 40°, 35°, 30°, 25°, 20°, 15°, 10°, 9°, 8°, 7°, 6°, 5°, 4°, 3°, 2°, 1°, 0.1°, 0.01°, or less. In some instances, the width, w, can be between a range of from about 100 micrometers (μm) to about 500 μm. In some instances, the width, w, can be between a range of from about 10 μm to about 200 μm. Alternatively, the width can be less than about 10 μm. Alternatively, the width can be greater than about 500 μm. In some instances, the flow rate of the aqueous fluid 408 entering the junction 406 can be between about 0.04 microliters (μL)/minute (min) and about 40 μL/min. In some instances, the flow rate of the aqueous fluid 408 entering the junction 406 can be between about 0.01 microliters (μL)/minute (min) and about 100 μL/min. Alternatively, the flow rate of the aqueous fluid 408 entering the junction 406 can be less than about 0.01 μL/min. Alternatively, the flow rate of the aqueous fluid 408 entering the junction 406 can be greater than about 40 μL/min, such as 45 μL/min, 50 μL/min, 55 μL/min, 60 μL/min, 65 μL/min, 70 μL/min, 75 μL/min, 80 μL/min, 85 μL/min, 90 μL/min, 95 μL/min, 100 μL/min, 110 μL/min, 120 μL/min, 130 μL/min, 140 μL/min, 150 μL/min, or greater. At lower flow rates, such as flow rates of about less than or equal to 10 microliters/minute, the droplet radius may not be dependent on the flow rate of the aqueous fluid 408 entering the junction 406.
In some instances, at least about 50% of the droplets generated can have uniform size. In some instances, at least about 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, or greater of the droplets generated can have uniform size. Alternatively, less than about 50% of the droplets generated can have uniform size.
The throughput of droplet generation can be increased by increasing the points of generation, such as increasing the number of junctions (e.g., junction 406) between aqueous fluid 408 channel segments (e.g., channel segment 402) and the reservoir 404. Alternatively or in addition, the throughput of droplet generation can be increased by increasing the flow rate of the aqueous fluid 408 in the channel segment 402.
Each channel segment of the plurality of channel segments 502 may comprise an aqueous fluid 508 that includes suspended beads 512. The reservoir 504 may comprise a second fluid 510 that is immiscible with the aqueous fluid 508. In some instances, the second fluid 510 may not be subjected to and/or directed to any flow in or out of the reservoir 504. For example, the second fluid 510 may be substantially stationary in the reservoir 504. In some instances, the second fluid 510 may be subjected to flow within the reservoir 504, but not in or out of the reservoir 504, such as via application of pressure to the reservoir 504 and/or as affected by the incoming flow of the aqueous fluid 508 at the junctions. Alternatively, the second fluid 510 may be subjected and/or directed to flow in or out of the reservoir 504. For example, the reservoir 504 can be a channel directing the second fluid 510 from upstream to downstream, transporting the generated droplets.
In operation, the aqueous fluid 508 that includes suspended beads 512 may be transported along the plurality of channel segments 502 into the plurality of junctions 506 to meet the second fluid 510 in the reservoir 504 to create droplets 516, 518. A droplet may form from each channel segment at each corresponding junction with the reservoir 504. At the junction where the aqueous fluid 508 and the second fluid 510 meet, droplets can form based on factors such as the hydrodynamic forces at the junction, flow rates of the two fluids 508, 510, fluid properties, and certain geometric parameters (e.g., w, h0, α, etc.) of the channel structure 500, as described elsewhere herein. A plurality of droplets can be collected in the reservoir 504 by continuously injecting the aqueous fluid 508 from the plurality of channel segments 502 through the plurality of junctions 506. Throughput may significantly increase with the parallel channel configuration of channel structure 500. For example, a channel structure having five inlet channel segments comprising the aqueous fluid 508 may generate droplets five times as frequently than a channel structure having one inlet channel segment, provided that the fluid flow rate in the channel segments are substantially the same. The fluid flow rate in the different inlet channel segments may or may not be substantially the same. A channel structure may have as many parallel channel segments as is practical and allowed for the size of the reservoir. For example, the channel structure may have at least about 2, 3, 4, 5, 6, 7, 8, 9, 10, 20, 30, 40, 50, 60, 70, 80, 90, 100, 150, 500, 250, 300, 350, 400, 450, 500, 600, 700, 800, 900, 1000, 1500, 5000 or more parallel or substantially parallel channel segments.
The geometric parameters, w, h0, and α, may or may not be uniform for each of the channel segments in the plurality of channel segments 502. For example, each channel segment may have the same or different widths at or near its respective channel junction with the reservoir 504. For example, each channel segment may have the same or different height at or near its respective channel junction with the reservoir 504. In another example, the reservoir 504 may have the same or different expansion angle at the different channel junctions with the plurality of channel segments 502. When the geometric parameters are uniform, beneficially, droplet size may also be controlled to be uniform even with the increased throughput. In some instances, when it is desirable to have a different distribution of droplet sizes, the geometric parameters for the plurality of channel segments 502 may be varied accordingly.
In some instances, at least about 50% of the droplets generated can have uniform size. In some instances, at least about 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, or greater of the droplets generated can have uniform size. Alternatively, less than about 50% of the droplets generated can have uniform size.
Each channel segment of the plurality of channel segments 602 may comprise an aqueous fluid 608 that includes suspended beads 612. The reservoir 604 may comprise a second fluid 610 that is immiscible with the aqueous fluid 608. In some instances, the second fluid 610 may not be subjected to and/or directed to any flow in or out of the reservoir 604. For example, the second fluid 610 may be substantially stationary in the reservoir 604. In some instances, the second fluid 610 may be subjected to flow within the reservoir 604, but not in or out of the reservoir 604, such as via application of pressure to the reservoir 604 and/or as affected by the incoming flow of the aqueous fluid 608 at the junctions. Alternatively, the second fluid 610 may be subjected and/or directed to flow in or out of the reservoir 604. For example, the reservoir 604 can be a channel directing the second fluid 610 from upstream to downstream, transporting the generated droplets.
In operation, the aqueous fluid 608 that includes suspended beads 612 may be transported along the plurality of channel segments 602 into the plurality of junctions 606 to meet the second fluid 610 in the reservoir 604 to create a plurality of droplets 616. A droplet may form from each channel segment at each corresponding junction with the reservoir 604. At the junction where the aqueous fluid 608 and the second fluid 610 meet, droplets can form based on factors such as the hydrodynamic forces at the junction, flow rates of the two fluids 608, 610, fluid properties, and certain geometric parameters (e.g., widths and heights of the channel segments 602, expansion angle of the reservoir 604, etc.) of the channel structure 600, as described elsewhere herein. A plurality of droplets can be collected in the reservoir 604 by continuously injecting the aqueous fluid 608 from the plurality of channel segments 602 through the plurality of junctions 606. Throughput may significantly increase with the substantially parallel channel configuration of the channel structure 600. A channel structure may have as many substantially parallel channel segments as is practical and allowed for by the size of the reservoir. For example, the channel structure may have at least about 2, 3, 4, 5, 6, 7, 8, 9, 10, 20, 30, 40, 50, 60, 70, 80, 90, 100, 150, 200, 250, 300, 350, 400, 450, 500, 600, 700, 800, 900, 1000, 1500, 5000 or more parallel or substantially parallel channel segments. The plurality of channel segments may be substantially evenly spaced apart, for example, around an edge or perimeter of the reservoir. Alternatively, the spacing of the plurality of channel segments may be uneven.
The reservoir 604 may have an expansion angle, a (not shown in
The reservoir 604 may have the same or different expansion angle at the different channel junctions with the plurality of channel segments 602. For example, a circular reservoir (as shown in
In some instances, at least about 50% of the droplets generated can have uniform size. In some instances, at least about 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, or greater of the droplets generated can have uniform size. Alternatively, less than about 50% of the droplets generated can have uniform size. The beads and/or biological particle injected into the droplets may or may not have uniform size.
An aqueous fluid 712 comprising a plurality of particles 716 may be transported along the channel segment 702 into the junction 706 to meet a second fluid 714 (e.g., oil, etc.) that is immiscible with the aqueous fluid 712 in the reservoir 704 to create droplets 720 of the aqueous fluid 712 flowing into the reservoir 704. At the junction 706 where the aqueous fluid 712 and the second fluid 714 meet, droplets can form based on factors such as the hydrodynamic forces at the junction 706, relative flow rates of the two fluids 712, 714, fluid properties, and certain geometric parameters (e.g., Δh, etc.) of the channel structure 700. A plurality of droplets can be collected in the reservoir 704 by continuously injecting the aqueous fluid 712 from the channel segment 702 at the junction 706.
A discrete droplet generated may comprise one or more particles of the plurality of particles 716. As described elsewhere herein, a particle may be any particle, such as a bead, cell bead, gel bead, biological particle, macromolecular constituents of biological particle, or other particles. Alternatively, a discrete droplet generated may not include any particles.
In some instances, the aqueous fluid 712 can have a substantially uniform concentration or frequency of particles 716. As described elsewhere herein (e.g., with reference to
In some instances, the second fluid 714 may not be subjected to and/or directed to any flow in or out of the reservoir 704. For example, the second fluid 714 may be substantially stationary in the reservoir 704. In some instances, the second fluid 714 may be subjected to flow within the reservoir 704, but not in or out of the reservoir 704, such as via application of pressure to the reservoir 704 and/or as affected by the incoming flow of the aqueous fluid 712 at the junction 706. Alternatively, the second fluid 714 may be subjected and/or directed to flow in or out of the reservoir 704. For example, the reservoir 704 can be a channel directing the second fluid 714 from upstream to downstream, transporting the generated droplets.
The channel structure 700 at or near the junction 706 may have certain geometric features that at least partly determine the sizes and/or shapes of the droplets formed by the channel structure 700. The channel segment 702 can have a first cross-section height, h1, and the reservoir 704 can have a second cross-section height, h2. The first cross-section height, h1, and the second cross-section height, h2, may be different, such that at the junction 706, there is a height difference of Δh. The second cross-section height, h2, may be greater than the first cross-section height, h1. In some instances, the reservoir may thereafter gradually increase in cross-section height, for example, the more distant it is from the junction 706. In some instances, the cross-section height of the reservoir may increase in accordance with expansion angle, β, at or near the junction 706. The height difference, Δh, and/or expansion angle, β, can allow the tongue (portion of the aqueous fluid 712 leaving channel segment 702 at junction 706 and entering the reservoir 704 before droplet formation) to increase in depth and facilitate decrease in curvature of the intermediately formed droplet. For example, droplet size may decrease with increasing height difference and/or increasing expansion angle.
The height difference, Δh, can be at least about 1 μm. Alternatively, the height difference can be at least about 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 25, 30, 35, 40, 45, 50, 60, 70, 80, 90, 100, 200, 300, 400, 500 μm or more. Alternatively, the height difference can be at most about 500, 400, 300, 200, 100, 90, 80, 70, 60, 50, 45, 40, 35, 30, 25, 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10, 9, 8, 7, 6, 5, 4, 3, 2, 1 μm or less. In some instances, the expansion angle, p, may be between a range of from about 0.5° to about 4°, from about 0.1° to about 10°, or from about 0° to about 90°. For example, the expansion angle can be at least about 0.01°, 0.1°, 0.2°, 0.3°, 0.4°, 0.5°, 0.6°, 0.7°, 0.8°, 0.9°, 1°, 2°, 3°, 4°, 5°, 6°, 7°, 8°, 9°, 10°, 15°, 20°, 25°, 30°, 35°, 40°, 45°, 50°, 55°, 60°, 65°, 70°, 75°, 80°, 85°, or higher. In some instances, the expansion angle can be at most about 89°, 88°, 87°, 86°, 85°, 84°, 83°, 82°, 81°, 80°, 75°, 70°, 65°, 60°, 55°, 50°, 45°, 40°, 35°, 30°, 25°, 20°, 15°, 10°, 9°, 8°, 7°, 6°, 5°, 4°, 3°, 2°, 1°, 0.1°, 0.01°, or less.
In some instances, the flow rate of the aqueous fluid 712 entering the junction 706 can be between about 0.04 microliters (μL)/minute (min) and about 40 μL/min. In some instances, the flow rate of the aqueous fluid 712 entering the junction 706 can be between about 0.01 microliters (μL)/minute (min) and about 100 μL/min. Alternatively, the flow rate of the aqueous fluid 712 entering the junction 706 can be less than about 0.01 μL/min. Alternatively, the flow rate of the aqueous fluid 712 entering the junction 706 can be greater than about 40 μL/min, such as 45 μL/min, 50 μL/min, 55 μL/min, 60 μL/min, 65 μL/min, 70 μL/min, 75 μL/min, 80 μL/min, 85 μL/min, 90 μL/min, 95 μL/min, 100 μL/min, 110 μL/min, 120 μL/min, 130 μL/min, 140 μL/min, 150 μL/min, or greater. At lower flow rates, such as flow rates of about less than or equal to 10 microliters/minute, the droplet radius may not be dependent on the flow rate of the aqueous fluid 712 entering the junction 706. The second fluid 714 may be stationary, or substantially stationary, in the reservoir 704. Alternatively, the second fluid 714 may be flowing, such as at the above flow rates described for the aqueous fluid 712.
In some instances, at least about 50% of the droplets generated can have uniform size. In some instances, at least about 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, or greater of the droplets generated can have uniform size. Alternatively, less than about 50% of the droplets generated can have uniform size.
While
The channel networks, e.g., as described above or elsewhere herein, can be fluidly coupled to appropriate fluidic components. For example, the inlet channel segments are fluidly coupled to appropriate sources of the materials they are to deliver to a channel junction. These sources may include any of a variety of different fluidic components, from simple reservoirs defined in or connected to a body structure of a microfluidic device, to fluid conduits that deliver fluids from off-device sources, manifolds, fluid flow units (e.g., actuators, pumps, compressors) or the like. Likewise, the outlet channel segment (e.g., channel segment 208, reservoir 604, etc.) may be fluidly coupled to a receiving vessel or conduit for the partitioned cells for subsequent processing. Again, this may be a reservoir defined in the body of a microfluidic device, or it may be a fluidic conduit for delivering the partitioned cells to a subsequent process operation, instrument or component.
The methods and systems described herein may be used to greatly increase the efficiency of single cell applications and/or other applications receiving droplet-based input. For example, following the sorting of occupied cells and/or appropriately-sized cells, subsequent operations that can be performed can include generation of extension and/or amplification products, purification (e.g., via solid phase reversible immobilization (SPRI)), and further processing (e.g., shearing, ligation of functional sequences, and subsequent generation of copies and/or complements of target sequences (e.g., amplification, such as via PCR)). These operations may occur in bulk (e.g., outside the partition). In the case where a partition is a droplet in an emulsion, the emulsion can be broken and the contents of the droplet pooled for additional operations. Additional reagents that may be co-partitioned along with the barcode bearing bead may include oligonucleotides to block ribosomal RNA (rRNA) and nucleases to digest genomic DNA from cells. Alternatively, rRNA removal agents may be applied during additional processing operations. The configuration of the constructs generated by such a method can help minimize (or avoid) sequencing of the poly-T sequence during sequencing and/or sequence the 5′ end of a polynucleotide sequence. Extension products, for example, first extension products and/or second extension products, may be subject to sequencing for sequence analysis. In some cases, amplification may be performed using the Partial Hairpin Amplification for Sequencing (PHASE) method.
A variety of applications require the evaluation of the presence and quantification of different biological particle or organism types within a population of biological particles, including, for example, microbiome analysis and characterization, environmental testing, food safety testing, epidemiological analysis, e.g., in tracing contamination or the like.
Computer Systems
The present disclosure provides computer systems that are programmed to implement methods of the disclosure.
The computer system 1001 includes a central processing unit (CPU, also “processor” and “computer processor” herein) 1005, which can be a single core or multi core processor, or a plurality of processors for parallel processing. The computer system 1001 also includes memory or memory location 1010 (e.g., random-access memory, read-only memory, flash memory), electronic storage unit 1015 (e.g., hard disk), communication interface 1020 (e.g., network adapter) for communicating with one or more other systems, and peripheral devices 1025, such as cache, other memory, data storage and/or electronic display adapters. The memory 1010, storage unit 1015, interface 1020 and peripheral devices 1025 are in communication with the CPU 1005 through a communication bus (solid lines), such as a motherboard. The storage unit 1015 can be a data storage unit (or data repository) for storing data. The computer system 1001 can be operatively coupled to a computer network (“network”) 1030 with the aid of the communication interface 1020. The network 1030 can be the Internet, an internet and/or extranet, or an intranet and/or extranet that is in communication with the Internet. The network 1030 in some cases is a telecommunication and/or data network. The network 1030 can include one or more computer servers, which can enable distributed computing, such as cloud computing. The network 1030, in some cases with the aid of the computer system 1001, can implement a peer-to-peer network, which may enable devices coupled to the computer system 1001 to behave as a client or a server.
The CPU 1005 can execute a sequence of machine-readable instructions, which can be embodied in a program or software. The instructions may be stored in a memory location, such as the memory 1010. The instructions can be directed to the CPU 1005, which can subsequently program or otherwise configure the CPU 1005 to implement methods of the present disclosure. Examples of operations performed by the CPU 1005 can include fetch, decode, execute, and writeback.
The CPU 1005 can be part of a circuit, such as an integrated circuit. One or more other components of the system 1001 can be included in the circuit. In some cases, the circuit is an application specific integrated circuit (ASIC).
The storage unit 1015 can store files, such as drivers, libraries and saved programs. The storage unit 1015 can store user data, e.g., user preferences and user programs. The computer system 1001 in some cases can include one or more additional data storage units that are external to the computer system 1001, such as located on a remote server that is in communication with the computer system 1001 through an intranet or the Internet.
The computer system 1001 can communicate with one or more remote computer systems through the network 1030. For instance, the computer system 1001 can communicate with a remote computer system of a user (e.g., operator). Examples of remote computer systems include personal computers (e.g., portable PC), slate or tablet PC's (e.g., Apple® iPad, Samsung® Galaxy Tab), telephones, Smart phones (e.g., Apple® iPhone, Android-enabled device, Blackberry®), or personal digital assistants. The user can access the computer system 1001 via the network 1030.
Methods as described herein can be implemented by way of machine (e.g., computer processor) executable code stored on an electronic storage location of the computer system 1001, such as, for example, on the memory 1010 or electronic storage unit 1015. The machine executable or machine readable code can be provided in the form of software. During use, the code can be executed by the processor 1005. In some cases, the code can be retrieved from the storage unit 1015 and stored on the memory 1010 for ready access by the processor 1005. In some situations, the electronic storage unit 1015 can be precluded, and machine-executable instructions are stored on memory 1010.
The code can be pre-compiled and configured for use with a machine having a processor adapted to execute the code, or can be compiled during runtime. The code can be supplied in a programming language that can be selected to enable the code to execute in a pre-compiled or as-compiled fashion.
Aspects of the systems and methods provided herein, such as the computer system 1001, can be embodied in programming. Various aspects of the technology may be thought of as “products” or “articles of manufacture” typically in the form of machine (or processor) executable code and/or associated data that is carried on or embodied in a type of machine readable medium. Machine-executable code can be stored on an electronic storage unit, such as memory (e.g., read-only memory, random-access memory, flash memory) or a hard disk. “Storage” type media can include any or all of the tangible memory of the computers, processors or the like, or associated modules thereof, such as various semiconductor memories, tape drives, disk drives and the like, which may provide non-transitory storage at any time for the software programming. All or portions of the software may at times be communicated through the Internet or various other telecommunication networks. Such communications, for example, may enable loading of the software from one computer or processor into another, for example, from a management server or host computer into the computer platform of an application server. Thus, another type of media that may bear the software elements includes optical, electrical and electromagnetic waves, such as used across physical interfaces between local devices, through wired and optical landline networks and over various air-links. The physical elements that carry such waves, such as wired or wireless links, optical links or the like, also may be considered as media bearing the software. As used herein, unless restricted to non-transitory, tangible “storage” media, terms such as computer or machine “readable medium” refer to any medium that participates in providing instructions to a processor for execution.
Hence, a machine readable medium, such as computer-executable code, may take many forms, including but not limited to, a tangible storage medium, a carrier wave medium or physical transmission medium. Non-volatile storage media include, for example, optical or magnetic disks, such as any of the storage devices in any computer(s) or the like, such as may be used to implement the databases, etc. shown in the drawings. Volatile storage media include dynamic memory, such as main memory of such a computer platform. Tangible transmission media include coaxial cables; copper wire and fiber optics, including the wires that comprise a bus within a computer system. Carrier-wave transmission media may take the form of electric or electromagnetic signals, or acoustic or light waves such as those generated during radio frequency (RF) and infrared (IR) data communications. Common forms of computer-readable media therefore include for example: a floppy disk, a flexible disk, hard disk, magnetic tape, any other magnetic medium, a CD-ROM, DVD or DVD-ROM, any other optical medium, punch cards paper tape, any other physical storage medium with patterns of holes, a RAM, a ROM, a PROM and EPROM, a FLASH-EPROM, any other memory chip or cartridge, a carrier wave transporting data or instructions, cables or links transporting such a carrier wave, or any other medium from which a computer may read programming code and/or data. Many of these forms of computer readable media may be involved in carrying one or more sequences of one or more instructions to a processor for execution.
The computer system 1001 can include or be in communication with an electronic display 1035 that comprises a user interface (UI) 1040 for providing, for example, results of sequencing analysis, etc. Examples of UIs include, without limitation, a graphical user interface (GUI) and web-based user interface.
Methods and systems of the present disclosure can be implemented by way of one or more algorithms. An algorithm can be implemented by way of software upon execution by the central processing unit 1005. The algorithm can, for example, perform sequencing, etc.
Devices, systems, compositions and methods of the present disclosure may be used for various applications, such as, for example, processing a single analyte (e.g., RNA, DNA, or protein) or multiple analytes (e.g., DNA and RNA, DNA and protein, RNA and protein, or RNA, DNA and protein) form a single cell. For example, a biological particle (e.g., a cell or cell bead) is partitioned in a partition (e.g., droplet), and multiple analytes from the biological particle are processed for subsequent processing. The multiple analytes may be from the single cell. This may enable, for example, simultaneous proteomic, transcriptomic and genomic analysis of the cell.
While preferred embodiments of the present invention have been shown and described herein, it will be obvious to those skilled in the art that such embodiments are provided by way of example only. It is not intended that the invention be limited by the specific examples provided within the specification. While the invention has been described with reference to the aforementioned specification, the descriptions and illustrations of the embodiments herein are not meant to be construed in a limiting sense. Numerous variations, changes, and substitutions will now occur to those skilled in the art without departing from the invention. Furthermore, it shall be understood that all aspects of the invention are not limited to the specific depictions, configurations or relative proportions set forth herein which depend upon a variety of conditions and variables. It should be understood that various alternatives to the embodiments of the invention described herein may be employed in practicing the invention. It is therefore contemplated that the invention shall also cover any such alternatives, modifications, variations or equivalents. It is intended that the following claims define the scope of the invention and that methods and structures within the scope of these claims and their equivalents be covered thereby.
This application is a continuation of U.S. application Ser. No. 16/434,605, filed Jun. 7, 2019, now U.S. Pat. No. 11,703,427, which claims the benefit of U.S. Provisional Patent Applications Nos. 62/689,651, filed Jun. 25, 2018, and 62/788,873, filed Jan. 6, 2019, which applications are entirely incorporated herein by reference.
| Number | Name | Date | Kind |
|---|---|---|---|
| 4124638 | Hansen | Nov 1978 | A |
| 4895909 | Sypniewski | Jan 1990 | A |
| 5185099 | Delpuech et al. | Feb 1993 | A |
| 5270183 | Corbett et al. | Dec 1993 | A |
| 5478893 | Ghosh et al. | Dec 1995 | A |
| 5736330 | Fulton | Apr 1998 | A |
| 5756334 | Perler et al. | May 1998 | A |
| 5846719 | Brenner et al. | Dec 1998 | A |
| 5900481 | Lough et al. | May 1999 | A |
| 5942609 | Hunkapiller et al. | Aug 1999 | A |
| 5994056 | Higuchi | Nov 1999 | A |
| 6033880 | Haff et al. | Mar 2000 | A |
| 6057149 | Burns et al. | May 2000 | A |
| 6123798 | Gandhi et al. | Sep 2000 | A |
| 6171850 | Nagle et al. | Jan 2001 | B1 |
| 6172218 | Brenner | Jan 2001 | B1 |
| 6176962 | Soane et al. | Jan 2001 | B1 |
| 6306590 | Mehta et al. | Oct 2001 | B1 |
| 6327410 | Walt et al. | Dec 2001 | B1 |
| 6379929 | Burns et al. | Apr 2002 | B1 |
| 6406848 | Bridgham et al. | Jun 2002 | B1 |
| 6409832 | Weigl et al. | Jun 2002 | B2 |
| 6492118 | Abrams et al. | Dec 2002 | B1 |
| 6524456 | Ramsey et al. | Feb 2003 | B1 |
| 6586176 | Trnovsky et al. | Jul 2003 | B1 |
| 6632655 | Mehta et al. | Oct 2003 | B1 |
| 6915679 | Chien et al. | Jul 2005 | B2 |
| 7041481 | Anderson et al. | May 2006 | B2 |
| 7115400 | Adessi et al. | Oct 2006 | B1 |
| 7129091 | Ismagilov et al. | Oct 2006 | B2 |
| 7268167 | Higuchi et al. | Sep 2007 | B2 |
| 7282370 | Bridgham et al. | Oct 2007 | B2 |
| 7294503 | Quake et al. | Nov 2007 | B2 |
| 7323305 | Leamon et al. | Jan 2008 | B2 |
| 7544473 | Brenner | Jun 2009 | B2 |
| 7622076 | Davies et al. | Nov 2009 | B2 |
| 7622280 | Holliger et al. | Nov 2009 | B2 |
| 7645596 | Williams et al. | Jan 2010 | B2 |
| 7708949 | Stone et al. | May 2010 | B2 |
| 7772287 | Higuchi et al. | Aug 2010 | B2 |
| 7776927 | Chu et al. | Aug 2010 | B2 |
| 7842457 | Berka et al. | Nov 2010 | B2 |
| 7910354 | Drmanac et al. | Mar 2011 | B2 |
| 7927797 | Nobile et al. | Apr 2011 | B2 |
| 7960104 | Drmanac et al. | Jun 2011 | B2 |
| 7968287 | Griffiths et al. | Jun 2011 | B2 |
| 8053192 | Bignell et al. | Nov 2011 | B2 |
| 8133719 | Drmanac et al. | Mar 2012 | B2 |
| 8168385 | Brenner | May 2012 | B2 |
| 8268564 | Roth et al. | Sep 2012 | B2 |
| 8273573 | Ismagilov et al. | Sep 2012 | B2 |
| 8298767 | Brenner et al. | Oct 2012 | B2 |
| 8304193 | Ismagilov et al. | Nov 2012 | B2 |
| 8318433 | Brenner | Nov 2012 | B2 |
| 8329407 | Ismagilov et al. | Dec 2012 | B2 |
| 8592150 | Drmanac et al. | Nov 2013 | B2 |
| 8658430 | Miller et al. | Feb 2014 | B2 |
| 8822148 | Ismagliov et al. | Sep 2014 | B2 |
| 8835358 | Fodor et al. | Sep 2014 | B2 |
| 8871444 | Griffiths et al. | Oct 2014 | B2 |
| 8889083 | Ismagilov et al. | Nov 2014 | B2 |
| 8975302 | Light et al. | Mar 2015 | B2 |
| 9012390 | Holtze et al. | Apr 2015 | B2 |
| 9029083 | Griffiths et al. | May 2015 | B2 |
| 9029085 | Agresti et al. | May 2015 | B2 |
| 9085798 | Chee | Jul 2015 | B2 |
| 9089844 | Hiddessen et al. | Jul 2015 | B2 |
| 9126160 | Ness et al. | Sep 2015 | B2 |
| 9156010 | Colston et al. | Oct 2015 | B2 |
| 9194861 | Hindson et al. | Nov 2015 | B2 |
| 9216392 | Hindson et al. | Dec 2015 | B2 |
| 9238206 | Rotem et al. | Jan 2016 | B2 |
| 9266104 | Link | Feb 2016 | B2 |
| 9290808 | Fodor et al. | Mar 2016 | B2 |
| 9328382 | Drmanac et al. | May 2016 | B2 |
| 9347059 | Saxonov | May 2016 | B2 |
| 9371598 | Chee | Jun 2016 | B2 |
| 9388465 | Hindson et al. | Jul 2016 | B2 |
| 9417190 | Hindson et al. | Aug 2016 | B2 |
| 9486757 | Romanowsky et al. | Nov 2016 | B2 |
| 9498761 | Holtze et al. | Nov 2016 | B2 |
| 9500664 | Ness et al. | Nov 2016 | B2 |
| 9567631 | Hindson et al. | Feb 2017 | B2 |
| 9593365 | Frisen et al. | Mar 2017 | B2 |
| 9623384 | Hindson et al. | Apr 2017 | B2 |
| 9637799 | Fan et al. | May 2017 | B2 |
| 9644204 | Hindson et al. | May 2017 | B2 |
| 9689024 | Hindson et al. | Jun 2017 | B2 |
| 9694361 | Bharadwaj et al. | Jul 2017 | B2 |
| 9701998 | Hindson et al. | Jul 2017 | B2 |
| 9764322 | Hiddessen et al. | Sep 2017 | B2 |
| 9824068 | Wong | Nov 2017 | B2 |
| 9868979 | Chee et al. | Jan 2018 | B2 |
| 9879313 | Chee et al. | Jan 2018 | B2 |
| 9946577 | Stafford et al. | Apr 2018 | B1 |
| 9951386 | Hindson et al. | Apr 2018 | B2 |
| 9957558 | Leamon et al. | May 2018 | B2 |
| 9975122 | Masquelier et al. | May 2018 | B2 |
| 10011872 | Belgrader et al. | Jul 2018 | B1 |
| 10017759 | Kaper et al. | Jul 2018 | B2 |
| 10030261 | Frisen et al. | Jul 2018 | B2 |
| 10059989 | Giresi et al. | Aug 2018 | B2 |
| 10221436 | Hardenbol et al. | Mar 2019 | B2 |
| 10221442 | Hindson et al. | Mar 2019 | B2 |
| 10253364 | Hindson et al. | Apr 2019 | B2 |
| 10273541 | Hindson et al. | Apr 2019 | B2 |
| 10323279 | Hindson et al. | Jun 2019 | B2 |
| 10347365 | Wong et al. | Jul 2019 | B2 |
| 10357771 | Bharadwaj et al. | Jul 2019 | B2 |
| 10395758 | Schnall-Levin | Aug 2019 | B2 |
| 10400280 | Hindson et al. | Sep 2019 | B2 |
| 10428326 | Belhocine et al. | Oct 2019 | B2 |
| 10450607 | Hindson et al. | Oct 2019 | B2 |
| 10533221 | Hindson et al. | Jan 2020 | B2 |
| 10544413 | Bharadwaj et al. | Jan 2020 | B2 |
| 10549279 | Bharadwaj et al. | Feb 2020 | B2 |
| 10557158 | Hardenbol et al. | Feb 2020 | B2 |
| 10590244 | Delaney et al. | Mar 2020 | B2 |
| 10745742 | Bent et al. | Aug 2020 | B2 |
| 10752949 | Hindson et al. | Aug 2020 | B2 |
| 10774374 | Frisen et al. | Sep 2020 | B2 |
| 10815525 | Lucero et al. | Oct 2020 | B2 |
| 10829815 | Bharadwaj et al. | Nov 2020 | B2 |
| 10837047 | Delaney et al. | Nov 2020 | B2 |
| 10874997 | Weitz et al. | Dec 2020 | B2 |
| 10995333 | Pfeiffer | May 2021 | B2 |
| 11371094 | Ryvkin et al. | Jun 2022 | B2 |
| 11459607 | Terry et al. | Oct 2022 | B1 |
| 11467153 | Belhocine et al. | Oct 2022 | B2 |
| 11655499 | Pfeiffer | May 2023 | B1 |
| 11703427 | Hindson et al. | Jul 2023 | B2 |
| 11845983 | Belhocine et al. | Dec 2023 | B1 |
| 11851683 | Maheshwari et al. | Dec 2023 | B1 |
| 11851700 | Bava et al. | Dec 2023 | B1 |
| 11952626 | Pfeiffer et al. | Apr 2024 | B2 |
| 20020005354 | Spence et al. | Jan 2002 | A1 |
| 20020051971 | Stuelpnagel et al. | May 2002 | A1 |
| 20020092767 | Bjornson et al. | Jul 2002 | A1 |
| 20020119455 | Chan | Aug 2002 | A1 |
| 20020127736 | Chou et al. | Sep 2002 | A1 |
| 20030036206 | Chien et al. | Feb 2003 | A1 |
| 20030075446 | Culbertson et al. | Apr 2003 | A1 |
| 20030124509 | Kenis et al. | Jul 2003 | A1 |
| 20030215862 | Parce et al. | Nov 2003 | A1 |
| 20050130188 | Walt et al. | Jun 2005 | A1 |
| 20050175702 | Muller-Schulte | Aug 2005 | A1 |
| 20050250147 | Macevicz | Nov 2005 | A1 |
| 20050266582 | Modlin et al. | Dec 2005 | A1 |
| 20050287572 | Mathies et al. | Dec 2005 | A1 |
| 20060177832 | Brenner | Aug 2006 | A1 |
| 20060275782 | Gunderson et al. | Dec 2006 | A1 |
| 20070020640 | McCloskey et al. | Jan 2007 | A1 |
| 20070042419 | Barany et al. | Feb 2007 | A1 |
| 20070148437 | Muller-Schulte | Jun 2007 | A1 |
| 20070172873 | Brenner et al. | Jul 2007 | A1 |
| 20070190543 | Livak | Aug 2007 | A1 |
| 20070196397 | Torii et al. | Aug 2007 | A1 |
| 20070264320 | Lee et al. | Nov 2007 | A1 |
| 20080056948 | Dale et al. | Mar 2008 | A1 |
| 20080166720 | Hsieh et al. | Jul 2008 | A1 |
| 20080242560 | Gunderson et al. | Oct 2008 | A1 |
| 20090011943 | Drmanac et al. | Jan 2009 | A1 |
| 20090047713 | Handique | Feb 2009 | A1 |
| 20090099041 | Church et al. | Apr 2009 | A1 |
| 20090131543 | Weitz et al. | May 2009 | A1 |
| 20090148961 | Luchini et al. | Jun 2009 | A1 |
| 20090155563 | Petsev et al. | Jun 2009 | A1 |
| 20090202984 | Cantor | Aug 2009 | A1 |
| 20090235990 | Beer | Sep 2009 | A1 |
| 20090269248 | Falb et al. | Oct 2009 | A1 |
| 20090286687 | Dressman et al. | Nov 2009 | A1 |
| 20100035254 | Williams | Feb 2010 | A1 |
| 20100069263 | Shendure et al. | Mar 2010 | A1 |
| 20100086914 | Bentley et al. | Apr 2010 | A1 |
| 20100086962 | Hunsley et al. | Apr 2010 | A1 |
| 20100105866 | Fraden et al. | Apr 2010 | A1 |
| 20100184928 | Kumacheva | Jul 2010 | A1 |
| 20100216153 | Lapidus et al. | Aug 2010 | A1 |
| 20100248991 | Roesler et al. | Sep 2010 | A1 |
| 20100304982 | Hinz et al. | Dec 2010 | A1 |
| 20110027771 | Deng | Feb 2011 | A1 |
| 20110217736 | Hindson | Sep 2011 | A1 |
| 20110305761 | Shum et al. | Dec 2011 | A1 |
| 20120071331 | Casbon et al. | Mar 2012 | A1 |
| 20120172259 | Rigatti et al. | Jul 2012 | A1 |
| 20120196288 | Beer | Aug 2012 | A1 |
| 20120219947 | Yurkovetsky et al. | Aug 2012 | A1 |
| 20130028812 | Prieto et al. | Jan 2013 | A1 |
| 20130109575 | Kleinschmidt et al. | May 2013 | A1 |
| 20140065234 | Shum et al. | Mar 2014 | A1 |
| 20140155295 | Hindson et al. | Jun 2014 | A1 |
| 20140221239 | Carman et al. | Aug 2014 | A1 |
| 20140227684 | Hindson | Aug 2014 | A1 |
| 20140272996 | Bemis | Sep 2014 | A1 |
| 20140274740 | Srinivasan et al. | Sep 2014 | A1 |
| 20140302503 | Lowe et al. | Oct 2014 | A1 |
| 20140338753 | Sperling et al. | Nov 2014 | A1 |
| 20140378322 | Hindson et al. | Dec 2014 | A1 |
| 20140378345 | Hindson et al. | Dec 2014 | A1 |
| 20150267191 | Steelman et al. | Sep 2015 | A1 |
| 20150361418 | Reed | Dec 2015 | A1 |
| 20150376605 | Jarosz et al. | Dec 2015 | A1 |
| 20150376609 | Hindson et al. | Dec 2015 | A1 |
| 20150376700 | Schnall-Levin et al. | Dec 2015 | A1 |
| 20150379196 | Schnall-Levin et al. | Dec 2015 | A1 |
| 20160008778 | Weitz et al. | Jan 2016 | A1 |
| 20160024558 | Hardenbol et al. | Jan 2016 | A1 |
| 20160024572 | Shishkin et al. | Jan 2016 | A1 |
| 20160053253 | Salathia et al. | Feb 2016 | A1 |
| 20160059204 | Hindson et al. | Mar 2016 | A1 |
| 20160060621 | Agresti et al. | Mar 2016 | A1 |
| 20160122753 | Mikkelsen et al. | May 2016 | A1 |
| 20160122817 | Jarosz et al. | May 2016 | A1 |
| 20160203196 | Schnall-Levin et al. | Jul 2016 | A1 |
| 20160232291 | Kyriazopoulou-Panagiotopoulou et al. | Aug 2016 | A1 |
| 20160244809 | Belgrader et al. | Aug 2016 | A1 |
| 20160281160 | Jarosz et al. | Sep 2016 | A1 |
| 20160289769 | Schwartz et al. | Oct 2016 | A1 |
| 20160314242 | Schnall-Levin et al. | Oct 2016 | A1 |
| 20160348093 | Price et al. | Dec 2016 | A1 |
| 20160376605 | Konzak et al. | Dec 2016 | A1 |
| 20170016041 | Greenfield et al. | Jan 2017 | A1 |
| 20170128937 | Hung et al. | May 2017 | A1 |
| 20170144161 | Hindson et al. | May 2017 | A1 |
| 20170145476 | Ryvkin et al. | May 2017 | A1 |
| 20170159109 | Zheng et al. | Jun 2017 | A1 |
| 20170235876 | Jaffe et al. | Aug 2017 | A1 |
| 20170260584 | Zheng et al. | Sep 2017 | A1 |
| 20180030515 | Regev et al. | Feb 2018 | A1 |
| 20180080075 | Brenner et al. | Mar 2018 | A1 |
| 20180105808 | Mikkelsen et al. | Apr 2018 | A1 |
| 20180273933 | Gunderson et al. | Sep 2018 | A1 |
| 20180312822 | Lee et al. | Nov 2018 | A1 |
| 20180312873 | Zheng | Nov 2018 | A1 |
| 20180340169 | Belhocine et al. | Nov 2018 | A1 |
| 20180340939 | Gaublomme et al. | Nov 2018 | A1 |
| 20180371545 | Wong et al. | Dec 2018 | A1 |
| 20190060890 | Bharadwaj et al. | Feb 2019 | A1 |
| 20190060905 | Bharadwaj et al. | Feb 2019 | A1 |
| 20190064173 | Bharadwaj et al. | Feb 2019 | A1 |
| 20190071656 | Chang et al. | Mar 2019 | A1 |
| 20190127731 | McDermott | May 2019 | A1 |
| 20190134633 | Bharadwaj et al. | May 2019 | A1 |
| 20190153532 | Bharadwaj et al. | May 2019 | A1 |
| 20190176152 | Bharadwaj et al. | Jun 2019 | A1 |
| 20190177800 | Boutet et al. | Jun 2019 | A1 |
| 20190323088 | Boutet et al. | Oct 2019 | A1 |
| 20190345636 | McDermott et al. | Nov 2019 | A1 |
| 20190352717 | Schnall-Levin | Nov 2019 | A1 |
| 20190367997 | Bent et al. | Dec 2019 | A1 |
| 20190376118 | Belhocine et al. | Dec 2019 | A1 |
| 20200005902 | Mellen et al. | Jan 2020 | A1 |
| 20200032335 | Martinez | Jan 2020 | A1 |
| 20200033237 | Hindson et al. | Jan 2020 | A1 |
| 20200033366 | Alvarado Martinez | Jan 2020 | A1 |
| 20200056223 | Bell | Feb 2020 | A1 |
| 20200105373 | Zheng | Apr 2020 | A1 |
| 20200263232 | Bell et al. | Aug 2020 | A1 |
| 20200291454 | Belhocine et al. | Sep 2020 | A1 |
| 20200407775 | Bharadwaj et al. | Dec 2020 | A1 |
| 20210190770 | Delaney et al. | Jun 2021 | A1 |
| 20210270703 | Abousoud | Sep 2021 | A1 |
| 20220162671 | Pfeiffer et al. | May 2022 | A1 |
| 20220403375 | Martinez | Dec 2022 | A1 |
| 20230167496 | Bava | Jun 2023 | A1 |
| 20240002914 | Pfeiffer et al. | Jan 2024 | A1 |
| Number | Date | Country |
|---|---|---|
| 1019496 | Sep 2004 | EP |
| 1841879 | Oct 2007 | EP |
| 1841879 | May 2009 | EP |
| 1967592 | Apr 2010 | EP |
| 2540389 | Jan 2013 | EP |
| 2635679 | Apr 2017 | EP |
| 2097692 | Nov 1982 | GB |
| 2097692 | May 1985 | GB |
| WO-8402000 | May 1984 | WO |
| WO-9530782 | Nov 1995 | WO |
| WO-9952708 | Oct 1999 | WO |
| WO-2000008212 | Feb 2000 | WO |
| WO-2001002850 | Jan 2001 | WO |
| WO-0114589 | Mar 2001 | WO |
| WO-0190418 | Nov 2001 | WO |
| WO-2001089787 | Nov 2001 | WO |
| WO-01089787 | Feb 2003 | WO |
| WO-2004002627 | Jan 2004 | WO |
| WO-2004065617 | Aug 2004 | WO |
| WO-2004069849 | Aug 2004 | WO |
| WO-2004091763 | Oct 2004 | WO |
| WO-2004069849 | Mar 2005 | WO |
| WO-2005021151 | Mar 2005 | WO |
| WO-2005049787 | Jun 2005 | WO |
| WO-2005082098 | Sep 2005 | WO |
| WO-2005082098 | Dec 2005 | WO |
| WO-2006040551 | Apr 2006 | WO |
| WO-2004065617 | Jun 2006 | WO |
| WO-2006078841 | Jul 2006 | WO |
| WO-2006096571 | Sep 2006 | WO |
| WO-2007081385 | Jul 2007 | WO |
| WO-2007081387 | Jul 2007 | WO |
| WO-2007089541 | Aug 2007 | WO |
| WO-2007133710 | Nov 2007 | WO |
| WO-2007140015 | Dec 2007 | WO |
| WO-2007147079 | Dec 2007 | WO |
| WO-2008021123 | Feb 2008 | WO |
| WO-2007147079 | Mar 2008 | WO |
| WO-2008109176 | Sep 2008 | WO |
| WO-2008121342 | Oct 2008 | WO |
| WO-2008134153 | Nov 2008 | WO |
| WO-2008150432 | Dec 2008 | WO |
| WO-2009011808 | Jan 2009 | WO |
| WO-2009015296 | Jan 2009 | WO |
| WO-2009085215 | Jul 2009 | WO |
| WO-2009152928 | Dec 2009 | WO |
| WO-2010033200 | Mar 2010 | WO |
| WO-2010104604 | Sep 2010 | WO |
| WO-2010117620 | Oct 2010 | WO |
| WO-2010148039 | Dec 2010 | WO |
| WO-2011028539 | Mar 2011 | WO |
| WO-2011047870 | Apr 2011 | WO |
| WO-2011056546 | May 2011 | WO |
| WO-2011066476 | Jun 2011 | WO |
| WO-2010148039 | Jul 2011 | WO |
| WO-2012048341 | Apr 2012 | WO |
| WO-2012061832 | May 2012 | WO |
| WO-2012083225 | Jun 2012 | WO |
| WO-2012083225 | Aug 2012 | WO |
| WO-2012106546 | Aug 2012 | WO |
| WO-2012109138 | Aug 2012 | WO |
| WO-2012112804 | Aug 2012 | WO |
| WO-2012112970 | Aug 2012 | WO |
| WO-2012116331 | Aug 2012 | WO |
| WO-2012142531 | Oct 2012 | WO |
| WO-2012142611 | Oct 2012 | WO |
| WO-2012149042 | Nov 2012 | WO |
| WO-2012166425 | Dec 2012 | WO |
| WO-2012167142 | Dec 2012 | WO |
| WO-2013019751 | Feb 2013 | WO |
| WO-2012116331 | Mar 2013 | WO |
| WO-2013036929 | Mar 2013 | WO |
| WO-2013055955 | Apr 2013 | WO |
| WO-2013096643 | Jun 2013 | WO |
| WO-2013126741 | Aug 2013 | WO |
| WO-2013134261 | Sep 2013 | WO |
| WO-2012106546 | Nov 2013 | WO |
| WO-2014028378 | Feb 2014 | WO |
| WO-2012142531 | May 2014 | WO |
| WO-2012166425 | May 2014 | WO |
| WO-2014108810 | Jul 2014 | WO |
| WO-2014165559 | Oct 2014 | WO |
| WO-2014108810 | Dec 2014 | WO |
| WO-2015015199 | Feb 2015 | WO |
| WO-2015044428 | Apr 2015 | WO |
| WO-2015164212 | Oct 2015 | WO |
| WO-2012142611 | Mar 2016 | WO |
| WO-2016040476 | Mar 2016 | WO |
| WO-2016061517 | Apr 2016 | WO |
| WO-2016061517 | Jun 2016 | WO |
| WO-2016126871 | Aug 2016 | WO |
| WO-2016168584 | Oct 2016 | WO |
| WO-2017015075 | Jan 2017 | WO |
| WO-2017066231 | Apr 2017 | WO |
| WO-2017139690 | Aug 2017 | WO |
| WO-2017180949 | Oct 2017 | WO |
| WO-2017184707 | Oct 2017 | WO |
| WO-2017197343 | Nov 2017 | WO |
| WO-2018039338 | Mar 2018 | WO |
| WO-2018091676 | May 2018 | WO |
| WO-2018119301 | Jun 2018 | WO |
| WO-2018119447 | Jun 2018 | WO |
| WO-2018172726 | Sep 2018 | WO |
| WO-2018191701 | Oct 2018 | WO |
| WO-2018213643 | Nov 2018 | WO |
| WO-2018226546 | Dec 2018 | WO |
| WO-2018236615 | Dec 2018 | WO |
| WO-2018237209 | Dec 2018 | WO |
| WO-2019028166 | Feb 2019 | WO |
| WO-2019040637 | Feb 2019 | WO |
| WO-2019083852 | May 2019 | WO |
| WO-2019084043 | May 2019 | WO |
| WO-2019084165 | May 2019 | WO |
| WO-2019108851 | Jun 2019 | WO |
| WO-2019113235 | Jun 2019 | WO |
| WO-2019118355 | Jun 2019 | WO |
| WO-2019126789 | Jun 2019 | WO |
| WO-2019148042 | Aug 2019 | WO |
| WO-2019152108 | Aug 2019 | WO |
| WO-2019157529 | Aug 2019 | WO |
| WO-2019165318 | Aug 2019 | WO |
| WO-2019169028 | Sep 2019 | WO |
| WO-2019169347 | Sep 2019 | WO |
| WO-2019191321 | Oct 2019 | WO |
| WO-2019217758 | Nov 2019 | WO |
| WO-2020005991 | Jan 2020 | WO |
| WO-2020028882 | Feb 2020 | WO |
| WO-2020041148 | Feb 2020 | WO |
| WO-2020142779 | Jul 2020 | WO |
| WO-2020167862 | Aug 2020 | WO |
| WO-2020167866 | Aug 2020 | WO |
| WO-2020168013 | Aug 2020 | WO |
| WO-2020198532 | Oct 2020 | WO |
| WO-2021046475 | Mar 2021 | WO |
| WO-2021133845 | Jul 2021 | WO |
| WO-2021207610 | Oct 2021 | WO |
| WO-2021212042 | Oct 2021 | WO |
| WO-2021222302 | Nov 2021 | WO |
| WO-2021222301 | Nov 2021 | WO |
| WO-2022103712 | May 2022 | WO |
| WO-2022182682 | Sep 2022 | WO |
| WO-2022182785 | Sep 2022 | WO |
| WO-2022271908 | Dec 2022 | WO |
| WO-2023076528 | May 2023 | WO |
| Entry |
|---|
| Therien-Aubin et al. Biomacromolecules, vol. 17, Sep. 10, 2016, pp. 3244-3251. |
| 10X Genomics, Inc. CG000153 Rev A. Chromium Single Cell DNA Reagent Kits User Guide. 2018. 62 pages. |
| 10X Genomics, Inc. CG000184 Rev A. Chromium Single Cell 3′ Reagent Kits v3 User Guide with Feature Barcoding Technology for CRISPR Screening. 2018. 70 pages. |
| 10X Genomics, Inc. CG000185 Rev B. Chromium Single Cell 3′ Reagent Kits User Guide with Feature Barcoding Technology for Cell Surface Protein. 2018. 66 pages. |
| 10X Genomics, Inc. CG000208 Rev E. Chromium Next GEM Single Cell V(D)J reagent Kits v1.1 User Guide with Feature Barcode Technology for Cell Surface Protein. 2020. 88 pages. |
| 10X Genomics, Inc. CG000209 Rev D. Chromium Next GEM Single Cell ATAC Reagent Kits v1.1 User Guide. 2020. |
| 10X Genomics, Inc. CG000239 Rev B. Visium Spatial Gene Expression Reagent Kits User Guide. 2020. |
| 10X Genomics, Inc. CG00026. Chromium Single Cell 3′ Reagent Kit User Guide. 2016. |
| 10X Genomics, Inc. LIT00003 Rev B Chromium Genome Solution Application Note. 2017. |
| Abate, A.R. et al. “Beating Poisson encapsulation statistics using close-packed ordering” Lab on a Chip (Sep. 21, 2009) 9(18):2628-2631. |
| Adamson et al., “Production of arrays of chemically distinct nanolitre plugs via repeated splitting in microfluidic devices”, Lab Chip 6(9): 1178-1186 (Sep. 2006). |
| Agasti, S.S. et al. “Photocleavable DNA barcode-antibody conjugates allow sensitive and multiplexed protein analysis in single cell” J Am Chem Soc (2012) 134(45):18499-18502. |
| Amini, S. et al. “Haplotype-resolved whole-genome sequencing by contiguity-preserving transposition and combinatorial indexing” Nature Genetics (2014) 46:1343-1349 doi:10.1038/ng.3119. |
| Anna et al.: Formation of dispersions using “flow focusing” in microchannels: Applied Physics Letters, vol. 82, No. 3, pp. 364-366 (2003). |
| Baret, “Surfactants in droplet-based microfluidics” Lab Chip (12(3):422-433 (2012). |
| Beer et al. On-Chip, Real-Time, Single-Copy Polymerase Chain Reaction in Picoliter Droplets. Anal Chem 79:8471-8475 (2007). |
| Braeckmans et al., Scanning the Code. Modern Drug Discovery. 2003:28-32. |
| Brenner, et al. “In vitro cloning of complex mixtures of DNA on microbeads: physical separation of differentially expressed cDNAs.” Proc Natl Acad Sci U S A. Feb. 15, 2000;97(4):1665-70. |
| Buchman GW, et al. Selective RNA amplification: a novel method using dUMP-containing primers and uracil DNA glycosylase. PCR Methods Appl. Aug. 1993; 3(1):28-31. |
| Burns, et al. An Integrated Nanoliter DNA Analysis Device. Science. Oct. 16, 1998;282(5388):484-7. |
| Burns, et al. Microfabricated structures for integrated DNA analysis. Proc Natl Acad Sci U S A. May 28, 1996; 93(11): 5556-5561. |
| Burns, et al. The intensification of rapid reactions in multiphase systems using slug flow in capillaries. Lab Chip. Sep. 2001;1(1):10-15. Epub Aug. 9, 2001. |
| Chen, et al. Chemical transfection of cells in picoliter aqueous droplets in fluorocarbon oil. Anal Chem. Nov. 15, 2011;83(22):8816-20. doi: 10.1021/ac2022794. Epub Oct. 17, 2011. |
| Chien et al. “Multiport flow-control system for lab-on-a-chip microfluidic devices”, Fresenius J. Anal Chem, 371:106-111 (Jul. 27, 2001). |
| Chu, et al. Controllable monodisperse multiple emulsions. Angew Chem Int Ed Engl. 2007;46(47):8970-4. |
| Clausell-Tormos et al., “Droplet-based microfluidic platforms for the encapsulation and screening of mammalian cells and multicellular organisms”, Chem. Biol. 15:427-437 (2008). |
| Co-pending U.S. Appl. No. 16/434,076, inventor Giresi; Paul, filed on Jun. 6, 2019. |
| Co-pending U.S. Appl. No. 16/434,084, inventor Giresi; Paul, filed on Jun. 6, 2019. |
| Co-pending U.S. Appl. No. 16/434,102, inventors Price; Andrew D. et al., filed on Jun. 6, 2019. |
| Co-pending U.S. Appl. No. 16/737,762, inventors Price; Andrew D. et al., filed on Jan. 8, 2020. |
| Co-pending U.S. Appl. No. 16/737,770, inventors Belhocine; Zahara Kamila et al., filed on Jan. 8, 2020. |
| Co-pending U.S. Appl. No. 16/789,273, inventors Maheshwari; Arundhati Shamoni et al., filed on Feb. 12, 2020. |
| Co-pending U.S. Appl. No. 17/014,909, inventor Giresi; Paul, filed on Sep. 8, 2020. |
| Co-pending U.S. Appl. No. 17/148,942, inventors Mcdermott; Geoffrey et al., filed on Jan. 14, 2021. |
| Co-pending U.S. Appl. No. 17/166,982, inventors Mcdermott; Geoffrey et al., filed on Feb. 3, 2021. |
| Co-pending U.S. Appl. No. 17/175,542, inventors Maheshwari; Arundhati Shamoni et al., filed on Feb. 12, 2021. |
| Co-pending U.S. Appl. No. 17/220,303, inventor Walter; Dagmar, filed on Apr. 1, 2021. |
| Co-pending U.S. Appl. No. 17/318,364, inventors Bava; Felice Alessio et al., filed on May 12, 2021. |
| Co-pending U.S. Appl. No. 17/381,612, inventor Martinez; Luigi Jhon Alvarado, filed on Jul. 21, 2021. |
| Co-pending U.S. Appl. No. 17/512,241, inventors Hill; Andrew John et al., filed on Oct. 27, 2021. |
| Co-pending U.S. Appl. No. 17/517,408, inventors Salmanzadeh; Alireza et al., filed on Nov. 2, 2021. |
| Co-pending U.S. Appl. No. 17/518,213, inventor Lund; Paul Eugene, filed on Nov. 3, 2021. |
| Co-pending U.S. Appl. No. 17/522,741, inventors Zheng; Xinying et al., filed on Nov. 9, 2021. |
| Co-pending U.S. Appl. No. 17/545,862, inventor Katherine; Pfeiffer, filed on Dec. 8, 2021. |
| Co-pending U.S. Appl. No. 17/573,350, inventor Corey; M. Nemec, filed on Jan. 11, 2022. |
| Co-pending U.S. Appl. No. 17/580,947, inventor Gibbons; Michael, filed on Jan. 21, 2022. |
| Co-pending U.S. Appl. No. 18/046,843, inventor Toh; Mckenzi, filed on Oct. 14, 2022. |
| Co-pending U.S. Appl. No. 18/152,650, inventor Shastry; Shankar, filed on Jan. 10, 2023. |
| Damean, et al. Simultaneous measurement of reactions in microdroplets filled by concentration gradients. Lab Chip. Jun. 21, 2009;9(12):1707-13. doi: 10.1039/b821021g. Epub Mar. 19, 2009. |
| Drmanac et al., Sequencing by hybridization (SBH): advantages, achievements, and opportunities. Adv Biochem Eng Biotechnol. 2002;77 :75-101. |
| Duffy et al., Rapid Protyping of Microfluidic Systems and Polydimethylsiloxane, Anal Chem 70:4974-4984 (1998). |
| Eastburn, et al. Ultrahigh-throughput mammalian single-cell reverse-transcriptase polymerase chain reaction in microfluidic droplets. Anal Chem. Aug. 20, 2013;85(16):8016-21. doi: 10.1021/ac402057q. Epub Aug. 8, 2013. |
| Esser-Kahn, et al. Triggered release from polymer capsules. Macromolecules. 2011; 44:5539-5553. |
| Gericke, et al. Functional cellulose beads: preparation, characterization, and applications. Chemical reviews 113.7 (2013): 4812-4836. |
| Guo, et al. Droplet microfluidics for high-throughput biological assays. Lab Chip. Jun. 21, 2012;12(12):2146-55. doi: 10.1039/c2lc21147e. Epub Feb. 9, 2012. |
| Gyarmati, et al. Reversible disulphide formation in polymer networks: a versatile functional group from synthesis to applications. European Polymer Journal. 2013; 49:1268-1286. |
| Hashimshony, et al. CEL-Seq: Single-Cell RNA-Seq by Multiplexed Linear Amplification. Cell Rep. Sep. 27, 2012;2(3):666-73. doi: 10.1016/j.celrep.2012.08.003. Epub Aug. 30, 2012. |
| Holtze, et al. Biocompatible surfactants for water-in-fluorocarbon emulsions. Lab Chip. Oct. 2008;8(10):1632-9. doi: 10.1039/b806706f. Epub Sep. 2, 2008. |
| Hug, et al. Measurement of the number of molecules of a single mRNA species in a complex mRNA preparation. J Theor Biol. Apr. 21, 2003;221(4):615-24. |
| Islam, et al. Highly multiplexed and strand-specific single-cell RNA 5′ end sequencing. Nat Protoc. Apr. 5, 2012;7(5):813-28. doi: 10.1038/nprot.2012.022. |
| Jaitin, et al. Massively parallel single-cell RNA-seq for marker-free decomposition of tissues into cell types. Science. Feb. 14, 2014;343(6172):776-9. doi: 10.1126/science.1247651. |
| Jarosz, M. et al. “Using 1ng of DNA to detect haplotype phasing and gene fusions from whole exome sequencing of cancer cell lines” Cancer Res (2015) 75(supp15):4742. |
| Kaper, et al. Supporting Information for “Whole-genome haplotyping by dilution, amplification, and sequencing.” Proc Natl Acad Sci U S A. Apr. 2, 2013;110(14):5552-7. doi: 10.1073/pnas.1218696110. Epub Mar. 18, 2013. |
| Kaper, et al. Whole-genome haplotyping by dilution, amplification, and sequencing. Proc Natl Acad Sci U S A. Apr. 2, 2013;110(14):5552-7. doi: 10.1073/pnas.1218696110. Epub Mar. 18, 2013. |
| Kenis, et al. Microfabrication Inside Capillaries Using Multiphase Laminar Flow Patterning. Science. 1999; 285:83-85. |
| Kivioja, et al. Counting absolute numbers of molecules using unique molecular identifiers. Nat Methods. Nov. 20, 2011;9(1):72-4. |
| Klein, et al. Droplet barcoding for single-cell transcriptomics applied to embryonic stem cells. Cell. May 21, 2015; 161:1187-1201. |
| Korlach et al., Methods in Enzymology, Real-Time DNA Sequencing from Single Polymerase Molecules, (2010) 472:431-455. |
| Koster et al., “Drop-based microfluidic devices for encapsulation of single cells”, Lab on a Chip The Royal Soc. of Chern. 8: 1110-1115 (2008). |
| Lagally, et al. Single-Molecular DNA Amplification and Analysis in an Integrated Microfluidic Device. Anal Chem. Feb. 1, 2001;73(3):565-70. |
| Lennon et al. A scalable, fully automated process for construction of sequence-ready barcoded libraries for 454. Genome Biology 11:R15 (2010). |
| Macosko, et al. Highly Parallel Genome-wide Expression Profiling of Individual Cells Using Nanoliter Droplets. Cell. May 21, 2015;161(5):1202-14. doi: 10.1016/j.cell.2015.05.002. |
| Madl, et al. “Bioorthogonal Strategies for Engineering Extracellular matrices”, Madal, Chritopher, Adv. Funct. Master. Jan. 19, 2018, vol. 28, 1706046, pp. 1-21. |
| Mair, et al. Injection molded microfluidic chips featuring integrated interconnects. Lab Chip. Oct. 2006;6(10):1346-54. Epub Jul. 31, 2006. |
| Mccoy, R. et al. “Illumina TruSeq Synthetic Long-Reads Empower De Novo Assembly and Resolve Complex, Highly-Repetitive Transposable Elements” PLOS (2014) 9(9):e1016689. |
| Microfluidic ChipShop. Microfluidic product catalogue. Oct. 2009. 48 pages. |
| Navin. The first five years of single-cell cancer genomics and beyond. Genome Res. Oct. 2015;25(10):1499-507. doi: 10.1101/gr.191098.115. |
| Nisisako, et al. Droplet formation in a microchannel network. Lab Chip. Feb. 2002;2(1):24-6. Epub Jan. 18, 2002. |
| Nisisako, T. et al. Droplet Formation in a Microchannel on PMMA Plate. Micro Total Analysis Systems. 2001. Kluwer Academic Publishers. pp. 137-138. |
| Nisisako, T. et al., Microfluidics large-scale integration on a chip for mass production of monodisperse droplets and particles, The Royal Society of Chemistry: Lab Chip, (Nov. 23, 2007) 8:287-293. |
| Novak, R. et al., “Single cell multiplex gene detection and sequencing using microfluidically generated agarose emulsions” Angew. Chem. Int. Ed. Engl. (2011) 50(2):390-395. |
| Orakdogen, N. “Novel responsive poly(N,N-dimethylaminoethyl methacrylate) gel beads: preparation, mechanical properties and pH-dependent swelling behavior” J Polym Res (2012) 19:9914. |
| Perrott, Jimmy. Optimization and Improvement of Emulsion PCR for the Ion Torrent Next-Generation Sequencing Platform. (2011) Thesis. |
| Peters, B.A. et al. Accurate whole-genome sequencing and haplotyping from 10 to 20 human cells. Nature, 487(7406):190-195 (Jul. 2012). |
| Plunkett, et al. Chymotrypsin responsive hydrogel: application of a disulfide exchange protocol for the preparation of methacrylamide containing peptides. Biomacromolecules. Mar.-Apr. 2005;6(2):632-7. |
| Priest, et al. Generation of Monodisperse Gel Emulsions in a Microfluidic Device, Applied Physics Letters, 88:024106 (2006). |
| Pushkarev et al. “Single-molecule sequencing of an individual human genome,” Nature Biotech (2009) 27:847-850. |
| Ramsey, J.M. “The burgeoning power of the shrinking laboratory” Nature Biotech (1999) 17:1061-1062. |
| Ramskold et al. (2012) “Full-length mRNA-Seq from single-cell levels of RNA and individual circulating tumor cells” Nature Biotechnology 30(8):777-782. |
| Roche. Using Multiplex Identifier (MID) Adaptors for the GS FLX Titanium Chemistry Basic MID Set Genome Sequencer FLX System, Technical Bulletin 004-2009, (Apr. 1, 2009) pp. 1-11. |
| Rotem, A. et al., “High-throughput single-cell labeling (Hi-SCL) for RNA-Seq using drop-based microfluidics” PLOS One (May 22, 2015) 0116328 (14 pages). |
| Rotem, A. et al. “Single Cell Chip-Seq Using Drop-Based Microfluidics” Abstract #50. Frontiers of Single Cell Analysis, Stanford University Sep. 5-7, 2013. |
| Saikia, et al. Simultaneous multiplexed amplicon sequencing and transcriptome profiling in single cells. Nat Methods. Jan. 2019;16(1):59-62. doi: 10.1038/s41592-018-0259-9. Epub Dec. 17, 2018. |
| Schubert, et al. Microemulsifying fluorinated oils with mixtures of fluorinated and hydrogenated surfactants. Colloids and Surfaces A; Physicochemical and Engineering Aspects, 84(1994) 97-106. |
| Seiffert, et al. Microfluidic fabrication of smart microgels from macromolecular precursors. 2010. Polymer. |
| Seiffert, S. et al., “Smart microgel capsules from macromolecular precursors” J. Am. Chem. Soc. (2010) 132:6606-6609. |
| Shah, et al. “Fabrication of mono disperse thermosensitive microgels and gel capsules in micro fluidic devices”, Soft Matter, 4:2303-2309 (2008). |
| Shendure, et al., Accurate Multiplex Polony Sequencing of an Evolved Bacterial Genome. Science 309.5741 (Sep. 2005): 1728-1732. XP002427180, ISSN: 0036-8075, DOI: 10.1126/SCIENCE.1117839. |
| Smith, et al. Highly-multiplexed barcode sequencing: an efficient method for parallel analysis of pooled samples. Nucleic Acids Research, 38(13): e142 (2010). |
| Song, et al. Reactions in droplets in microfluidic channels. Angew Chem Int Ed Engl. Nov. 13, 2006;45(44):7336-56. |
| Thaxton, C.S. et al. “A Bio-Bar-Code Assay Based Upon Dithiothreitol Oligonucleotide Release” Anal Chem (2005) 77:8174-8178. |
| Theberge, et al. Microdroplets in microfluidics: an evolving platform for discoveries in chemistry and biology. Angew Chem Int Ed Engl. Aug. 9, 2010;49(34):5846-68. doi: 10.1002/anie.200906653. |
| Thorsen, et al. Dynamic pattern formation in a vesicle-generating microfluidic device. Physical Review Letters. American Physical Society. 2001; 86(18):4163-4166. |
| Tonelli, et al. Perfluoropolyether functional oligomers: unusual reactivity in organic chemistry. Journal of fluorine chemistry. 2002; 118(1)107-121. |
| Turchinovich, et al. “Capture and Amplification by Tailing and Switching (CATS): An Ultrasensitive Ligation-Independent Method for Generation of DNA Libraries for Deep Sequencing from Picogram Amounts of DNA and RNA.” RNA Biology 11.7 (2014): 817-828. PMC. Web. Nov. 13, 2017. |
| Uttamapinant, et al. Fast, cell-compatible click chemistry with copper-chelating azides for biomolecular labeling. Angew. Chem. Int. End. Engl., Jun. 11, 2012: 51(24) pp. 5852-5856. |
| Wagner, et al. Biocompatible fluorinated polyglycerols for droplet microfluidics as an alternative to PEG-based copolymer surfactants. Lab Chip. Jan. 7, 2016;16(1):65-9. doi: 10.1039/c5lc00823a. Epub Dec. 2, 2015. |
| Weigl, et al. Microfluidic Diffusion-Based Separation and Detection. Science. 1999; pp. 346-347. |
| Williams, et al. Amplification of complex gene libraries by emulsion PCR. Nature Methods. 2006;3(7):545-50. |
| Yousuff, et al. Microfluidic Platform for Cell Isolation and Manipulation Based on Cell Properties. Micromachines (Basel). Jan. 2017; 8(1): 15; 1-26. Published online Jan. 4, 2017. doi: 10.3390/mi8010015. |
| Zhang, et al. One-step fabrication of supramolecular microcapsules from microfluidic droplets. Science. Feb. 10, 2012;335(6069):690-4. doi: 10.1126/science.1215416. |
| Zheng, et al. Massively parallel digital transcriptional profiling of single cells. Nat Commun. Jan. 16, 2017;8:14049. doi: 10.1038/ncomms14049. |
| Zheng, X.Y. et al. “Haplotyping germline and cancer genomes with high-throughput linked-read sequencing” Nature Biotech (Feb. 1, 2016) 34(3):303-311. |
| Zhu, et al. Reverse transcriptase template switching: a SMART approach for full-length cDNA library construction. Biotechniques. Apr. 2001;30(4):892-7. |
| Co-pending U.S. Appl. No. 18/392,684, inventors Fernandes; Sunjay Jude et al., filed on Dec. 21, 2023. |
| Co-pending U.S. Appl. No. 18/643,684, inventor Bava; Felice Alessio, filed on Apr. 23, 2024. |
| Number | Date | Country | |
|---|---|---|---|
| 20240003785 A1 | Jan 2024 | US |
| Number | Date | Country | |
|---|---|---|---|
| 62788873 | Jan 2019 | US | |
| 62689651 | Jun 2018 | US |
| Number | Date | Country | |
|---|---|---|---|
| Parent | 16434605 | Jun 2019 | US |
| Child | 18205397 | US |
