The present invention pertains to the medical field, in particular to the discovery of a biomarker and methods for detecting the presence of sepsis in a biological sample by measuring the nuclease activity of said sample.
Sepsis is defined by the Third International Consensus Definitions for Sepsis and Septic Shock (Sepsis-3) as “a life-threatening organ dysfunction caused by a dysregulated host response to infection”.1 If not recognized early and managed promptly, it can lead to septic shock, multiple organ failure and death. Any type of infectious pathogen can potentially cause sepsis. Antimicrobial resistance is a major factor determining clinical unresponsiveness to treatment and rapid evolution to sepsis and septic shock. Sepsis patients with resistant pathogens have been found to have a higher risk of hospital mortality. Anyone affected by an infection can progress to sepsis conditions but some vulnerable populations such as elderly people, pregnant women, neonates, hospitalized patients, and people with HIV/AIDS, liver cirrhosis, cancer, kidney disease, autoimmune diseases and no spleen, are at higher risk.2 Septic shock is the deadliest form of sepsis, resulting in higher mortality rates. Sepsis is frequently under-diagnosed at an early stage, when is still potentially reversible. Sepsis is treatable, and timely implementation of targeted interventions improves outcomes. Unfortunately, diagnosis of sepsis remains a major challenge for many reasons. Sepsis diagnosis still relies on nonspecific physiological criteria (including changes in temperature and heart and respiration rates) and culture-based pathogen detection. This results in diagnostic uncertainty, therapeutic delays, the misuse and overuse of antibiotics and many other problems that increase mortality.3,4
Furthermore, in the case of patients suspected of sepsis, clinical microbiology tests may be negative but when positive often require two or more days to produce actionable results. These microbiologic data suffer from significant numbers of false-positives and false-negatives when attempting to identify the actual microbial cause of sepsis.5,6 Importantly, in clinical practice, only 14-30% of sepsis cases are clinical-relevantly diagnosed by blood culture. Thus, early diagnosis of sepsis is critical because intervention could potentially have the greatest patient benefit early in the disease course. Antibiotic administration decreases the likelihood of death by 7.6% per hour.7 Unfortunately, standard diagnostic tests are not available within this critical time frame to allow focused, effective, and potentially life-saving medical interventions.
Recently, biochemical tests (immunoassays and molecular methods), especially, PCR-based approaches are gaining popularity as they are fast and very sensitive. However, they typically require a positive blood culture and the species and strain-specific primers that may or may not be available for a particular organism. Other faster adjunct standard hematological analyses used in routine clinical practice have low sensitivity and specificity, particularly in neonatal patients. Recently, biomarkers such as C-reactive protein (CRP), procalcitonin (PCT), and the neutrophil marker CD64 have made their way into sepsis evaluations, with somewhat limited success.
This underscores the need for novel types of biomarkers that could in theory characterize the host response to rule in/out infection, and to more accurately distinguish patients who are truly septic or at risk of becoming septic and assist with the appropriate use of antibiotics.
In the context of the present invention “polynucleotide” is understood as oligonucleotide substrate (single or double stranded) with the capability to recognize a specific nuclease activity derived from bacteria or mammalian cells. The polynucleotide composition consists of natural nucleic acids (DNA and/or RNA), chemically modified nucleic acids or a combination of both.
In the context of the present invention “capture tag” is understood as the modification at the end of the “polynucleotide” that allows binding with the capture molecule and/or the reporter molecule. This binding is carried out by interaction of antigen-antibody or a similar biorecognition process.
In the context of the present invention “capture molecule” is understood as recognition molecule (e.g. biotin antibody) immobilized in the detection line of the lateral flow assay, with the capability to bind one of the ends of the polynucleotide probe that was previously modified with a capture tag (e.g. biotin).
In the context of the present invention “reporter molecule” is understood as a) the molecule that provides the readout of the system and consists of a latex/gold nanoparticle, carbon particle or a similar colorimetric molecule, functionalized with a capture molecule (e.g. antibody) that recognizes the polynucleotide probe via a capture tag. b) The reporter molecule consists of a latex/gold nanoparticle that is functionalized with the polynucleotide probe, at one of its ends by coupling chemistry (e.g. amine), while the other end is modified with a capture tag (e.g. biotin).
In the context of the present invention “lateral flow assay” is understood as a simple methodology to detect, in a specific manner, the presence or absence of a target analyte in a given matrix sample. This type of assay eliminates the need for specialized and costly equipment used in conventional laboratories. Lateral flow technology is used as point of care tool (fast and in situ), in a large range of applications, from the widely used home pregnancy test to more specialized tests for clinical, food safety, environmental and industrial applications.
In the context of the present invention “a backing card” is understood as the material where all the membranes (sample, conjugate, membrane and wicking pads) are mounted and connected to each other. At the same time, the backing card provides better stability and handling to the lateral flow device.
In the context of the present invention “a sample pad” is understood as the element of the device where the sample is introduced or deposited and acts as a sponge that holds the sample fluid. Subsequently, the sample fluid is transported to the membrane by capillary action.
In the context of the present invention “the conjugate pad” is understood as the part of the device where the reporter molecules are dispensed. The reporter molecules are immediately released from the conjugate pad upon contact with the sample fluid.
In the context of the present invention “a membrane” is understood as the material (e.g. nitrocellulose) that transports the sample and provides support to the capture molecules (antibodies, aptamers etc.) and allows or enhances their binding capabilities.
In the context of the present invention “a wicking pad” is understood as element in the system that retains all the assay material acting as a waste container.
In the context of the present invention “biological sample” is understood, as used in this invention, to be any sample of, relating to, caused by, or affecting life or living organisms, biological processes, such as growth and digestion. Examples of a biological sample may include, but are not limited to cell culture, cell culture supernatant, saliva, tears, urine, blood, serum, plasma, sweat, vaginal fluids, semen, feces, mucous, breast milk, ascites, lymph, pleural effusion, synovial fluid, bone marrow, cerebro-spinal fluid, and washings from bodily cavities (e.g., bronchial lavage), hair, tissue, bones, or teeth, the biological sample is a liquid sample. Liquid means a state of matter with definite volume but no definite shape at 25° C., like water.
As illustrated throughout the examples of the present specification, the measurement of the inhibitory activity of a subject's RBCs-RI (Red Blood Cells cytosolic ribonuclease inhibitors) on the blood/serum RNases is a novel way of differentiating healthy from septic blood and represents a valid biomarker with great clinical potential. In particular, the effect of lysing a blood biological sample, preferably the erythrocytes, and the subsequent release of the cytosolic ribonuclease inhibitors (RI), in particular the Blood ERythrocyte-derived RNase Inhibitors (BERRI), provides for a significant decrease in RNase activity in comparison to control (non-lysed samples) or uninfected or healthy samples, while little effect is observed in septic samples, showing that the reduction of nuclease activity in comparison to control (non-lysed samples) or uninfected or healthy samples is due to the specific inhibition, by the BERRI, of the blood/serum RNases, in particular of the RNase A type endonucleases.
In this sense, as shown herein in
In the context of a subject suffering from sepsis or septic shock, the Blood ERythrocyte-derived RNase Inhibitors (BERRI), which are cytoplasmic proteins present in the erythrocytes of the subject that inhibit a variety of RNases, contain cysteine residues, all of which occur in the reduced state. Modification of the thiol groups (SH—) of these cysteine residues inactivate the protein and greatly increases its susceptibility to proteolysis. In fact, oxidation of the thiol groups in RI occurs within the erythrocytes when these are exposed to a disease associated to an oxidative stress such as sepsis or septic shock. That is, when a subject suffers a disease that causes ROS (reactive oxygen species) within the erythrocytes, such as is the case with sepsis, the Blood ERythrocyte-derived RNase Inhibitors (BERRI) are degraded by proteolysis in a way that when lysed, the lysed samples will not inhibit the RNase activity.
In order to demonstrate the above, we have induced an oxidative insult in whole blood samples that simulates the effect of the oxidation produced by a septicaemia. For this purpose, the thiol-specific oxidant, diamide, has been used, this compound leads to the loss of RI activity. Specifically, whole blood (from healthy volunteers, stored at 4° C.) were incubated with diamide at room temperature for 1 h (9 μL blood+1 μL of various concentrations of diamide, along with a control without diamide). After oxidation, blood samples were treated either with Lysis Buffer (that only lysed erythrocytes) or PBS to a final dilution of 1:10. Next, nuclease activity was assayed using the SP7 probe (Fluorescence). The results are shown herein in
As exemplified in
Consequently, the present invention shows that the effect of lysing a blood biological sample that leads to the release of the Blood ERythrocyte-derived RNase Inhibitors (BERRI) from the lysed erythrocytes, and the subsequent measurement of RNase activity in said lysed samples, provides for a method of identifying whether a subject, from which the biological sample was obtained, does or not suffer from oxidative stress, in particular from sepsis or septic shock.
Additional kinetic studies using blood samples (lysed and not lysed) were used to demonstrate the catalytic behaviour of blood nucleases.
Thus, the present invention provides for the first time the utility and specificity of the Blood ERythrocyte-derived RNase Inhibitor Activity (BERRIA) as a biomarker for detecting sepsis in lysed blood or serum biological samples. It is noted that BERRIA is not only a biomarker for detecting sepsis in lysed blood or serum biological samples but also for detecting oxidative stress and any disease associated to oxidative stress. In particular, the detection of an absence or of a significant reduction of the inhibitory activity in comparison to control (non-lysed samples) or uninfected or healthy samples of such RNase inhibitor over the RNase A family of endoribonucleases present in such isolated blood or serum biological sample, is indicative of sepsis in the human subject from which the biological sample was obtained. Therefore, determining or measuring the nuclease activity of the canonical RNase A family of endoribonucleases in a lysed isolated blood or serum biological sample obtained from a human subject, can serve as a biomarker of sepsis, and of oxidative stress, in said subject. That is, if no nuclease activity or a significant reduction of the activity in comparison to control or reference values (from i.e. non-lysed samples) or from uninfected or healthy samples is detected or measured in a blood or serum biological sample, then this is indicative that the BERRI is active and thus that the subject from which the biological sample was obtained does not suffer from sepsis. By contrast, a significant nuclease activity, similar or with no statistical significance in comparison to control or reference values (from i.e. non-lysed samples) or from uninfected or healthy samples, is detected or measured in such lysed blood or serum biological sample, then this is indicative that the BERRI is not active and thus that the subject from which the biological sample was obtained does suffer from sepsis.
In this sense, it is noted that the protein ribonuclease A (RNase A) was initially extracted from the bovine pancreas and is one of the best-characterized mammalian proteins in the literature. Over time, several other proteins with significant sequence homology were identified in mammals and other vertebrates, allowing assembly of the vertebrate-specific RNase A superfamily [Beintema, J. J.; Kleinedam, R. G]. The ribonuclease A superfamily: General discussion. Cell. Mol. LifeSci. 1998, 54, 825-832. Sorrentino, S. The eight human “canonical” ribonucleases: Molecular diversity, catalytic properties, and special biological actions of the enzyme proteins. FEBS Lett. 2010, 584, 2194-2200]. The members of this superfamily can be distinguished from other exo- and endoribonucleases, which show different distributions and properties. In humans, eight secreted RNases have been described and are generally referred to as the canonical RNases (RNases 1 to 8, Int. J. Mol. Sci. 2016, 17, 1278).
These proteins show a tertiary structure that is stabilized by eight disulphide bridges, with the exception of RNase 5, which has six cysteine residues. Two histidine residues and one lysine residue determine the catalytic activity of these RNases; the lysine residue lies within the common invariant sequence motif CKxxNTF. Each RNase initially contains an N-terminal signal sequence that directs protein biosynthesis within the endoplasmic reticulum, with its final form being secretory. Moreover, the N-terminal portion of the mature extracellular RNase appears to be required for antimicrobial activity [Lander, E. S.; Linton, L. M.; Birren, B.; Nusbaum, C.; Zody, M. C.; Baldwin, J.; Devon, K.; Dewar, K.; Doyle, M.; Fitz Hugh, W.; et al. Initial sequencing and analysis of the human genome. Nature 2001, 409, 860-921]. This feature was demonstrated by generating N-terminus-derived peptides that showed similar antimicrobial activity.
In the present invention, we thus show that the determination of the nuclease activity of any of the eight secreted RNases (1 to 8) pertaining to the RNAse A family of endoribonucleases, generally referred to as the canonical RNases, in an isolated blood or serum sample, specifically in a lysed blood sample, serve as a biomarker of sepsis, or of oxidative stress, in a human subject. Hence, a first aspect of the invention refers to the in vitro use of the levels of the nuclease activity of the canonical RNases (RNases 1 to 8) pertaining to the RNAse A family of endoribonucleases, in an isolated blood or serum biological sample, preferably in a lysed blood sample, as a biomarker of sepsis, or of oxidative stress, in a human subject.
It is noted that such determination, as reflected in the above paragraph, is not limited to lysed blood or serum samples but can also be done, as reflected in
It is further noted that any reference to the human RNase A type family containing the eight canonical members, shall be understood as to any of the following: human RNase 1 (hRNase1), human RNase 2 (hRNase2) also named eosinophil-derived neurotoxin (EDN), human RNase 3 (hRNase3) also named eosinophil cationic protein (ECP), human RNase 4 (hRNase4), human RNase 5 (hRNase5) also called angiogenin/ANG, human RNase 6 (hRNase6) also named RNase k6, human RNase 7 (hRNase7), and human RNase 8 (hRNase8).
In addition, a second aspect of the invention refers to the in vitro use of the Blood ERythrocyte-derived RNase Inhibitor Activity (BERRIA) in an isolated blood or serum biological sample as a biomarker of sepsis, or of oxidative stress, in a human subject, more particularly, in an isolated blood biological sample wherein the erythrocytes have been lysed thus releasing the Blood ERythrocyte-derived RNase Inhibitor.
A third aspect of the invention refers to an in vitro method for determining whether a subject, preferably a human subject, suffers or not from sepsis, or from oxidative stress, that comprises the following steps:
wherein such comparison shall indicate whether the subject does or not suffer from sepsis, or from oxidative stress, based on the RNase activity detected in the isolated sample.
A preferred embodiment of the third aspect of the invention refers to an in vitro method for determining whether a subject, preferably a human subject, suffers or not from sepsis, or from oxidative stress, that comprises
wherein such comparison shall indicate whether the subject does or not suffer from sepsis or from oxidative stress based on the RNase activity detected in the isolated sample, in particular, wherein if no nuclease activity or a significant reduction of the activity is detected or measured in the blood sample in comparison to the control reference or reference value, then this is indicative that the Blood ERythrocyte-derived RNase Inhibitors are active and thus that the subject from which the biological sample was obtained does not suffer from sepsis or from oxidative stress, by contrast, if a significant nuclease activity is detected or measured in such lysed blood sample, then this is indicative that the BERRI is not active and thus that the subject from which the biological sample was obtained does suffer from sepsis or from oxidative stress.
In a preferred embodiment of the third aspect or of any of its preferred embodiments, the reference value, or value range, used is representative of the RNase activity in control (such as non-lysed samples obtained from the same subject or from another suitable source) or healthy uninfected samples, so that if the measurement of the RNase activity detected in the isolated sample is similar or not statistically different to the reference value, or value range, or the value obtained from a healthy or uninfected sample, this is indicative that the subject suffers from sepsis or oxidative stress, otherwise (if there is a significant reduction) this is indicative that the subject does not suffer from sepsis or from oxidative stress.
In another preferred embodiment, a reference value, or value range, is used in the method of the third aspect of the invention which is representative of the RNase activity in samples with sepsis so that if the measurement of the RNase activity detected in the isolated biological sample being tested is significantly lower than the reference value, or the value range, then this is indicative that the subject does not suffer from sepsis, otherwise this is indicative that the subject does suffer from sepsis.
In yet another preferred embodiment, the sepsis is due or caused by a microbial, bacterial, viral or fungi infection.
It is noted that the measurement or detection of the nuclease activity of any of the previous aspects of the present invention can be performed by a large variety of techniques. In this sense, it is known that RNase A family members bind and catalyze the phosphodiester bond cleavage in a wide variety of nucleotide substrates of different lengths and sequences. Past studies have suggested substrate preferences at the pyrimidine binding site for some of the human RNases: hRNase1 showed a preference for cytosine (C) over uridine (U), while hRNases 2, 3, 4 and 6 showed a preference for U over C [Sorrentino S. The eight human “canonical” ribonucleases: Molecular diversity, catalytic properties, and special biological actions of the enzyme proteins. FEBS Letters. 2010; 584(11):2194-200. https://doi.org/10.1016/j.febslet.2010.04.018 PMID: 20388512. Prats-Ejarque G, Arranz-Trullen J, Blanco J A, Pulido D, Nogues M V, Moussaoui M, et al. The first crystal structure of human RNase 6 reveals a novel substrate-binding and cleavage site arrangement. Biochemical Journal. 2016; 473(11):1523-36]. In contrast, the purine binding site was shown to prefer adenosine (A) in these hRNases [1, 2, 3].
In order to measure the nuclease activity, we have identified a number of oligonucleotide substrates (Table 2) as specific substrates for the blood RNases, particularly RNase A family of riboendonucleases, that are able to discriminate between an infected blood (septic) sample and a healthy blood sample. These oligonucleotide substrates share the following features in common: i) short length, ii) RNA pyrimidines as preferred substrate for sepsis-derived nucleases, iii) chimeric sequences, containing nucleoside analogues modified and natural RNA pyrimidines nucleotides (unmodified). A cleavage region between 1 to 3 RNA pyrimidines flanked by chemically modified nucleotides increase the specificity for RNases derived from sepsis. It is particularly noted that the cleavage motif —CUC— in (SP7 of table 2) flanked by chemically modified nucleotides has shown increased specificity and sensitivity for detecting sepsis-derived RNases.
Therefore, to measure the nuclease activity according to any of the previous aspects, oligonucleotide substrates (from hereinafter oligonucleotides of the invention) can be used that comprise i) 5-30 nucleotides in length, and ii) a cleavage region between 1 to 3 RNA pyrimidines, preferably flanked by chemically modified nucleotides, wherein such cleavage region is susceptible of being cleaved by any of the eight canonical secreted RNases (1 to 8) pertaining to the RNase A family. Preferably, said cleavage region is selected from any of those identified in bold in table 2. More preferably, the cleavage motif is —CUC—, as identified in SP7 of table 2, preferably flanked by chemically modified nucleotides that has shown increased specificity and sensitivity for detecting sepsis-derived RNases. Still more preferably, said oligonucleotide substrates are selected from any of the list identified in Table 2.
In addition, in a preferred embodiment of the third aspect of the invention, the method of the third aspect of the invention comprises measuring or detecting a fluorescence signal of the sample after contacting said biological sample with at least one probe comprising an oligonucleotide of the invention, a fluorophore operably linked to the oligonucleotide, and a quencher operably linked to the oligonucleotide, wherein the oligonucleotide is capable of being cleaved by the RNase A family of endoribonucleases in said sample, preferably after the blood lysis, more preferably after erythrocyte lysing. It is noted that such oligonucleotides of the invention useful to practice this specific embodiment of the invention are described in WO2013033436, which is herein fully incorporated by reference. Preferably, such oligonucleotides of the invention, are capable of being cleaved by the RNase A family of endoribonucleases and comprise chemically modified RNA flanked with a fluorophore on one end and a fluorescence quencher on the other end. Upon cleavage of the probes by the RNase A family of endoribonucleases, the fluorophore diffuses away from the quencher and exhibits fluorescence.
Thus, in one preferred embodiment, the method of the third aspect of the invention can be thus implemented by detecting a fluorescence signal, such method would preferably comprise: 1) incubating an oligonucleotide substrate of the invention in the sample, for a time sufficient for cleavage of the Substrates(s) by the RNase A family of endoribonucleases in the, preferably lysed, blood or the serum sample obtained from the said blood sample, wherein the Substrate(s) comprises a single-stranded nucleic acid molecule containing at least one RNA pyrimidine residue at an internal position that functions as a nuclease (e.g., ribonuclease) cleavage site, a fluorescence reporter group on one side of the cleavage sites, and a fluorescence-quenching group on the other side of the cleavage site, and 2) detection of a fluorescence signal, wherein detection of a fluorescence signal indicates that the RNase A family of endoribonucleases cleavage event has occurred, and, therefore, the sample contains said nuclease (e.g., ribonuclease) activity which is indicative that the subject does not suffer of sepsis. The oligonucleotide substrates of the invention are compatible with different detection modalities (e.g., fluorometry). For this specific embodiment, it is mandatory that the Substrate oligonucleotides of the invention comprise a fluorescent reporter group and a quencher group in such physical proximity that the fluorescence signal from the reporter group is suppressed by the quencher group. Cleavage of the Substrate with a nuclease (e.g., ribonuclease) enzyme leads to strand cleavage and physical separation of the reporter group from the quencher group. Separation of reporter and quencher eliminates quenching, resulting in an increase in fluorescence emission from the reporter group. When the quencher is a so-called “dark quencher”, the resulting fluorescence signal can be detected by direct visual inspection (provided the emitted light includes visible wavelengths).
On the other hand, to enable visual detection of the method of the third aspect of the invention implemented by detecting a fluorescence signal, the quenching group is itself not capable of fluorescence emission, being a “dark quencher”. Use of a “dark quencher” eliminates the background fluorescence of the intact Substrate that would otherwise occur as a result of energy transfer from the reporter fluorophore. In one embodiment, the fluorescence quencher comprises dabcyl (4-(4′-dimethylaminophenylazo)benzoic acid). In one embodiment, the fluorescence quencher is comprised of QSY™-7 carboxylic acid, succinimidyl ester (N,N′-dimethyl-N,N′-diphenyl-4-((5-t-butoxycarbonylaminopentyl)aminocarbonyl)piperidinylsulfonerhodamine; a diarylrhodamine derivative from Molecular Probes, Eugene, Oreg.). Any suitable fluorophore may be used as reporter provided its spectral properties are favorable for use with the chosen quencher. A variety of fluorophores can be used as reporters, including but not limited to, fluorescein, tetrachlorofluorescein, hexachlorofluorescein, rhodamine, tetramethylrhodamine, Cy-dyes, Texas Red, Bodipy dyes, and Alexa dyes.
The method of the third aspect of the invention implemented by detecting a fluorescent signal may thus preferably proceed in two steps. First, the, preferably lysed, test biological sample is mixed with the Substrate oligonucleotide of the invention and incubated. Substrate can be mixed alone with the test sample or will be mixed with an appropriate buffer. Second, detection of fluorescence is performed. As fluorescence above background indicates fluorescence emission of the reaction product, i.e. the cleaved Substrate, detection of such fluorescence indicates that RNase activity is present in the test sample. The method provides that this step can be done with unassisted visual inspection. In particular, visual detection can be performed using a standard ultraviolet (UV) light source of the kind found in most molecular biology laboratories to provide fluorescence excitation. Substrates of the invention can also be utilized in assay formats in which detection of Substrate cleavage is done using a multi-well fluorescence plate reader or a tube fluorometer.
On a still further note, as previously indicated, the detection or measurement of the nuclease activity of any of the aspects of the present invention can be performed by a variety of techniques, wherein for example a further technique is by detecting or measuring the nuclease activity, preferably visually, by using a lateral flow device. It is noted that such lateral flow devices useful to practice the present invention, in particular the method of the third aspect of the invention, are thoroughly described in WO2019/043187, which is herein wholly incorporated by reference.
Thus, the present invention further includes lateral flow devices and kits containing the same for implementing any of the aspects herein indicated, in particular the method of the third aspect of the invention. Such kits may optionally contain one or more of: a positive control nuclease, a probe substrate, DNase/RNase-free water, a buffer, and other reagents.
Lateral Flow tests, also known as Lateral Flow Immunochromatography (LFIC) tests or just Lateral Flow Immunoassays (LFI), are simple small devices intended to detect a target analyte in a given sample. These tests are simple, fast, and cheap and do not require specialized and costly equipment nor qualified personnel. Even though the LFIC technology was born in the early 80s, it is still going to be a major player in the next decades in the rapid test industry. In the beginning, LFIC tests were exclusively used in medical diagnostics (either for home testing, point of care testing, or laboratory use) but their presence in other areas are now very common. The first application was the well-known home pregnancy test. Although there are several commercially available semi-quantitative tests, LFIC tests are mainly qualitative tests and they just give a positive or negative result based on the presence or absence of the target analyte at a specific concentration.
The technology is based on a series of capillary beds with porous materials that has the capacity to transport liquids spontaneously. When the target analyte is big (e.g. a protein) a sandwich format is commonly used. Usually, the first element (the conjugate pad) has stored the so-called colloids, a dried format of bioactive particles (mainly gold nanoparticles or latex microparticles bound to a specific antibody against the target analyte) in a salt-sugar matrix. This conjugate pad usually acts also as the sample pad and it contains everything to facilitate the recognition of the target antigen by the colloid. Once the conjugate pad is soaked by the sample, the fluid dissolves the salt-sugar matrix and the colloids. Therefore, the sample and the colloid mix while they flow through the porous structure. In this way, the analyte binds to the colloid and the complex is transported into the nitrocellulose membrane. This material has one specific area called test line where a third molecule (usually another different antibody against the target analyte) has been immobilized. When the complex (colloid-antigen) reaches the test line it gets bound to this antibody and after a while, when more and more fluid has flown, colloids accumulate, and the test line acquires colour. Typically, there is also a control line (after the test line) that captures the colloids that have not bound to the test line. In the control line, a fourth molecule (could be an antibody against the Fc fraction of the first antibody) is adsorbed and its color acquisition ensures the test functionality. After passing these reaction zones the fluid enters the final porous material, the wick or sink that simply acts as a waste container.
In this sense, we have developed specific LFPC strips that can detect non-degraded polynucleotide probes in a given sample. Accordingly, the presence of a polynucleotide probe will give rise to a coloured test line, which according to the methodology of the third aspect of the invention indicates that the subject does not suffer from sepsis. On the other hand, if a specific nuclease is present in the, preferably lysed, sample it will eventually produce a complete degradation of the polynucleotide probe, and consequently, no test line will be seen, which according to the methodology of the third aspect of the invention indicates that the subject does suffer from sepsis, preferably since if a lysed sample is used, no BERRI activity would be detected in the sample. Such system shall provide for a rapid and sensitive molecular approach useful for implementing the method of the third aspect of the invention. For that purpose, the present invention thus provides for an in vitro use of a polynucleotide sequence of the invention susceptible of being cleaved by the RNAse A family of endoribonucleases present in a, preferably lysed, blood or the serum obtained from that said blood biological sample, in a lateral flow assay.
To practice the present invention, a large variety of different lateral flow system devices could be used, however, two devices to which the present invention is not limited to are herein provided just for illustrative purposes:
A first lateral flow device comprising a support suitable for lateral flow, which in turn comprises:
Preferably, said first lateral flow device is comprised in a kit which further comprises a probe comprising an oligonucleotide sequence of the invention susceptible of being cleaved by the RNAse A family of endoribonucleases, wherein the oligonucleotide sequence is characterized by comprising a capture tag and a reporter molecule at each end of the sequence, for determining whether a subject suffers or not from sepsis.
A second lateral flow device comprising a support suitable for lateral flow, which in turn comprises:
Preferably, said second lateral flow device is comprised in a kit which further comprises a probe comprising an oligonucleotide sequence of the invention susceptible of being cleaved by the RNase A family of endoribonucleases, wherein the oligonucleotide sequence is characterized by comprising two capture tags at each end of the sequence, wherein each capture tag is different from the other and wherein one of the capture tags is capable of binding a reporter molecule, for determining whether a subject suffers or not from sepsis.
In certain embodiments, the said polynucleotides sequences of the invention susceptible of being cleaved by the RNase A family of endoribonucleases, are short oligonucleotide probes (substrate) composed of nucleic acids and flanked with a capture tag on one end and i) a reporter molecule or ii) a further capture tag capable of binding the reporter molecule located in the conjugate pad of a support of a lateral flow device, on the other end. Upon cleavage of the probes by the RNase A family of endoribonucleases in the, preferably lysed, blood or the serum obtained from that said blood sample, the capture tag is released away from the reporter molecule indicating the presence of said nucleases in a biological sample in a lateral flow device. Cleavage of the oligonucleotide by the RNase A family of endoribonuclease leads to strand cleavage and physical separation of the capture group from the reporter group. Separation of reporter and capture eliminates the possibility of detecting a signal in the test line of the lateral flow device thus resulting in the detection of nuclease activity in the sample. The absence of the resulting signal can be detected by direct visual inspection of the lateral flow device.
In addition, in a further specific embodiment of the present invention, the implementation of method of the third aspect of the invention, comprises: 1) incubating a synthetic Substrate or mixture of oligonucleotide Substrates of the invention in the, preferably lysed, sample, for a time sufficient for cleavage of the Substrate(s) by the RNase A family of endoribonucleases, wherein the Substrate(s) comprises a single-stranded (or double-stranded) nucleic acid molecule containing at least one RNA pyrimidines residue or chemically modified RNA pyrimidines residue at an internal position that functions as a nuclease (RNase A family of endoribonuclease) cleavage site, a capture tag on one end and i) a reporter molecule or ii) a further capture tag capable of binding the reporter molecule located in the conjugate pad of a support of a lateral flow device, on the other end, and 2) detecting the result, preferably visually, in particular whether the substrate has been cleaved or not by a test sample, by using a lateral flow device.
Yet another preferred embodiment of the present invention, relates to the in vitro use of a kit or a lateral flow device which comprises:
for implementing the methodology of the third aspect of the invention.
Preferably, said lateral flow device comprises:
Also preferably, said lateral flow device comprises:
Also preferably, said lateral flow device comprises:
It is further noted that the lateral flow device or kit described in the third aspect of the invention may further comprise a control line.
Furthermore, the method of the third aspect of the invention can be, and is preferably, implemented for diagnostic or screening purposes, such as for diagnosing or screening whether a subject suffers or not from sepsis or from oxidative stress. Further implementations of the method of the third aspect of the invention such as for monitoring treatment response to sepsis or oxidative stress are encompassed within the present invention.
Finally, it is noted that the detection of the activity of the RNase A family of endoribonucleases of the aspects of the invention can be performed by further techniques such as nuclear magnetic resonance (NMR).
The following examples merely serve to illustrate the present invention but do not limit the same.
The nuclease activity profile of paired blood and serum samples infected with various pathogens was screened with a library of 12 probes, either naked (DNA or RNA) or containing chemical modifications at the 2′position of at the sugar ribose (2′-O methyl and 2′-fluoro). The nuclease activity of infected blood with Methicillin resistant Staphylococcus epidermidis (MRSE), Methicillin sensitive Staphylococcus aureus (MSSA) and Klebsiella oxytoca and PBS was measured by fluorescence intensity (arbitrary units).
The results are shown in
A second round of screening was conducted in serum samples infected with MRSE or MSSA, similarly as described in example 1, but using an extended library containing the 80 nucleic acid probe sequences shown in Table 1 and a healthy blood sample (N1) as control sample. The results are shown in
Further, in this Example, healthy (N1-3) or pathogen infected blood samples (infected with MRSE, Klebsiella, E. coli-1, E. coli-2 or MSSA) were screened for nuclease activity, before or after blood lysis using the extended library containing the 80 nucleic acid probe sequences of Table 1.
The results are shown in
Lastly,
In view of the results of the screening of the previous example, 12 specific probes that resulted in better discrimination between the negative and positive groups were selected for further experiments. These probes are shown in Table 2 (SP1-SP12). Next, a larger number of blood samples that tested positive (microbiologically) for a variety of bacteria (n=70) along with normal, healthy controls (n=11) were tested using the 3 first identified probes from Table 2 (SP1-3).
To further characterize the type of nucleases whose activity was being measured, the 3 specific probes (SP1, SP2 and Sp3) used in the previous example were used with various chelators and cations and in negative and positive-infected lysed blood samples. The results are shown in
As it can be seen in
To further measure the nuclease activity in blood samples from control, healthy individuals (N1 and N2) and patients diagnosed with sepsis (P1 and P2), an assay using the specifically identified probe (SP1) (Table 2) was performed. In this case, both red blood cells (RBCs) and white blood cells (WBCs) were lysed for nuclease activity measurement. The results are shown in
To confirm that the reduction of nuclease activity is due to the specific inhibition of the RNAse A type nucleases in blood, a commercially available ribonuclease inhibitor (RNaselnh) was also used in the sample. As it can be appreciated from
In this Example, RBCs lysates from the negative samples (N1, N2, N3 and N4) or positive samples (P3 and P4) were used as source of RI and were added to the whole blood samples, along with the commercial RNaselnh. The results are shown in
The examples above show that the measurement of inhibitory activity of RBCs-RI on the blood RNases is a novel way of differentiating healthy from septic blood and represents a valid biomarker with great clinical potential.
The utility and specificity of the Blood ERythrocyte-derived RNase Inhibitor Activity (BERRIA) biomarker for detecting sepsis was further tested in a cohort of patient samples: 24 positive samples for sepsis or septic shock (S1 to S24) and 32 negative samples for sepsis (confirmed as inflammation) (S25 to S56) along with 19 healthy controls (N1 to N19) using the nucleic acid sequences SP1 and SP7 in Table 2.
The results are shown in
Lastly, the diagnostic accuracy of SP7 was compared with the clinically accepted markers of sepsis: Procalcitonin and CRP. The results are shown in
Once SP7 was successfully tested in clinical samples in the fluorescence format, the probe was next synthesized for the lateral flow optimization.
Number | Date | Country | Kind |
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20382550.0 | Jun 2020 | EP | regional |
Filing Document | Filing Date | Country | Kind |
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PCT/EP2021/067254 | 6/23/2021 | WO |