Patent Grant
9029144
The present invention relates generally to methods for the isolation and propagation of cells. More specifically, the invention relates to methods for the isolation and propagation of large numbers of cells for use, for example, in biotherapeutic devices, such as devices for renal replacement therapy, for the treatment of acute renal failure, (ARF), acute tubular necrosis (ATN), multi-organ failure (MOF), sepsis, cardiorenal syndrome (CRS) and end-stage renal disease (ESRD).
Acute renal failure and chronic renal disease (CRD) are distinctly different disease processes. The current therapy for both disorders is suboptimal. ARF arises from toxic or ischemic (usually simultaneous) tubule damage from antibiotics, chemo-therapeutic agents, or shock during infectious or major operative procedures. The development of ARF in a hospitalized patient results in a 5 to 8 fold higher risk of death (Chertow et al. (1998), A
Sepsis frequently leads to ATN, ARF and MOF with approximately 700,000 cases per year in the United States. The most serious forms of sepsis result in severe sepsis (250,000 annual cases in the U.S.) or septic shock (85,000 annual cases in the U.S.) with mortality rates of greater than 30 and 50 percent, respectively (Schrier et al. (2004), N. E
CRS is defined as a clinical disorder in patients with concomitant cardiac congestive heart failure (CHF) and renal dysfunction in which the therapy to relieve the congestive symptoms of heart failure, usually with diuretics, results and is limited by further declines in renal function. Currently, five million people in the U.S. have cardiac failure, with 350,000 of them having CRS (Shlipak et al. (2004), C
Unlike ARF, CRD is an irreversible process of progressive kidney damage commonly from diabetes and hypertension which leads to end-stage renal disease. Patients with ESRD on dialysis continue to have major medical, social and economic problems and a life expectancy of only 4-5 years (Iglehart (1993), N. E
Enhancement of cell propagation, such as enhancing kidney cell expansion, can provide the biomass for cell-based therapeutic products for clinical indications of various diseases. It is contemplated that the methods and protocols described herein will lead to improving the quality of, and potentially extend, life for patients with impaired renal function. To this end, the therapeutic potential of a bioartificial renal tubule has been demonstrated with a hollow fiber-based renal assist device (RAD) (Humes et al. (2003), B
In one aspect, the present invention relates to a method for enhanced propagation of cells from a sample. The method includes promoting expansion of precursor cells from the sample, thereby to enhance propagation of a cell population from the sample. This aspect can include one or more of the following features. The sample can be a kidney sample. The precursor cells can be renal precursor cells. During the procedure, the precursor cells can be maintained in an undifferentiated state. The method can increase the number of precursor cells relative to the number present in the sample. The method can include dilution of cells in the sample and/or dilution of cells in a culture or subculture derived from the sample. The enhanced propagation cell population can include renal cells. The method can retain non-adherent cells, for example during changes of growth media during culturing. The method can further include administering retinoic acid.
In another aspect, the present invention relates to a method for enhanced propagation of renal cells from a kidney sample. The method includes (a) treating the kidney sample to produce tissue fragments having a dimension of about 212 μm or less in size; (b) harvesting at least a portion of the tissue fragments by centrifugation at greater than about 50×(g) to produce a pelleted slurry; (c) applying a portion of the pelleted slurry produced in step (b) to a solid support (e.g., a tissue culture plate or other growth surface) and culturing the cells from the pelleted slurry in the absence of retinoic acid and under conditions to permit the renal cells to attach to the solid support and divide; (d) harvesting the cells produced in step (c) prior to reaching confluence on the solid support; (e) applying a portion of the cells harvested in step (d) to a solid support and culturing the cells in the absence of retinoic acid and under conditions to permit the renal cells to attach to the solid support and divide; and (f) harvesting the cells produced in step (e) to produce a propagated population of renal cells. Non adherent cells can be retained when culture growth medium is changed. This aspect of the invention can also have any one or more of the features described above or below.
In another aspect, the method for the enhanced propagation of cells from a sample includes separating the sample into fragments about 212 μm or less in size to produce a slurry; centrifuging the slurry at greater than about 50×(g) to produce a pelleted slurry; creating a culture by applying to a growth surface (e.g., a tissue culture plate or other solid support) about 10 μL or less of the pelleted slurry per square centimeter of surface area of the surface on which the cells in the culture will grow (e.g., a tissue culture plate or other solid support); and subculturing, typically prior to confluence, about 8.7×104 or fewer cells from the culture per square centimeter of surface area on which the subculture is to be grown. In certain embodiments, the method further includes adding retinoic acid after the subculturing step. In certain embodiments, the sample is a kidney sample. In certain embodiments, the non-adherent cells are retained when culture or subculture growth medium is changed. The subculturing step can be repeated with subsequent subcultures such that these further subcultures are initiated by subculturing about 8.7×104 or fewer cells from the previous subculture per square centimeter of surface area on which the further subculture is to be grown. This aspect of the invention can also have any one or more of the features described above or below.
It is to be understood that the features of the various embodiments described herein are not mutually exclusive and may exist in various combinations and permutations. The foregoing aspects and embodiments of the invention may be more fully understood by reference to the following figures, detailed description and claims.
The invention is illustrated, but is not limited by, the annexed drawings.
Cell therapy, for example, renal cell therapy, promises to improve the survival and overall health of patients suffering from various diseases, including ARF, ATN, MOF, sepsis, CRS, and ESRD. To this end, therapeutic biological devices addressing the physiologic component of cell replacement therapy, such as renal cell replacement therapy, are being developed. Use of this technology in a clinical setting requires manufacture of devices containing available human, or other mammalian, cells. For acute care situations involving cell-based therapeutic devices, it is beneficial to have such devices readily available. As such, these devices typically use xenogenic or allogenic cells in order to have the cells available for rapid use (either to add to a device as needed or to pre-populate a device during manufacturing). For example, as described in Examples 1 and 2, methods according to the invention can be used to provide such cells. However, for chronic care situations, autogenic cells (in addition to xenogenic or allogenic cells) may be used to populate cell-based therapeutic devices. For example, as described in Example 3, tissue from a kidney biopsy can be used according to the methods of the invention to provide cells to populate such devices. Thus, a patient's own cells can be sampled, grown, and used in a cell-based therapeutic device used to treat that patient.
The overall goal of the present invention is to employ cell propagation protocols which result in the isolation and propagation of more human cells, such as renal epithelial cells, than was possible with historic methods. Specifically, the enhanced propagation methods of the present invention seek to promote the expansion of precursor cells to increase cell yield in the resulting cell population. For example, the methods described herein apply conditions that stimulate and/or amplify precursor cells, thus increasing the number of precursor cells in a sample. In some embodiments, the methods involve one or more of (1) more efficient enzymatic digestion of a sample tissue, (2) use of smaller tissue particle sizes (e.g., about 212 μm or less), (3) the use of higher centrifugal forces on a digested tissue sample to generate a pelleted slurry that includes more precursor cells, (4) dilution of cell density (e.g., dilution during initial plating (plating density) and/or dilution during subculturing (passage ratio)), and (5) maintaining passaged cells at subconfluent conditions during expansion. The methods also can involve partial media changes and techniques to retain non-adherent cells to promote a proliferative environment for precursor cells. Retinoic acid to induce terminal differentiation can be added to cell cultures produced from enhanced propagation methods of the invention (i.e., enhanced propagation cell cultures) just prior to their use to maintain the precursor cells in an undifferentiated state. The retinoic acid, or other differentiation factor, can be absent from culturing and/or subculturing steps. It is contemplated that any one, or any combination of two or more, of these condition variables may be employed to enhance cell propagation.
In certain embodiments, a tissue sample, such as a kidney tissue sample, is separated into suitable fragments (e.g., by enzymatically digesting the sample and/or by sieving a fragmented sample into fragments about 212 μm or less in size) to generate a slurry. The slurry is centrifuged (e.g., at greater than about 50×(g)) to generate pelleted slurry. Typically, pelleted slurry is a cell suspension in which the cells are more concentrated than the original slurry (centrifugation that produces the pelleted slurry can be followed by removal of supernatant). The resulting pelleted slurry is plated (or otherwise provided to a growth surface) to generate a culture. For example, about 10 μL or less of the pelleted slurry is applied per square centimeter of surface area of the tissue culture plate on which the culture is to be grown (or other surface on which the cells in the culture will grow). Cells from the culture, typically prior to confluence, are then subcultured (e.g., using about 8.7×104 or fewer cells from the culture per square centimeter of surface area on which the subculture is to be grown). This subculturing step can be repeated by subculturing, typically prior to confluence, the previous subculture (e.g., using about 8.7×104 or fewer cells from the previous subculture per square centimeter of surface area on which the further subculture is to be grown). Any of these steps can be done in the absence of a differentiating factor (e.g., retinoic acid).
The enhanced propagation methods and resulting cell populations are beneficial in the cell therapy field. Accordingly, methods according to the present invention combined retention of therapeutic potential with manufacturing feasibility for human REC. Cell yield per human donor kidney is contemplated to meet projected clinical need. Certain embodiments of the present invention encompass optimizing human REC integration into bioartificial renal epithelial cell-based devices currently being developed using animal cells, followed by evaluation of efficacy using pre-clinical large animal models of chronic renal failure, ARF and sepsis.
It is contemplated that the methods described herein are applicable to a variety of cells, including various renal cells. For example, in instances where cells are harvested from organs (either from living donors having given proper consent or from cadavers whose tissues were properly donated), it is contemplated that cells can be expanded according to the methods described herein. It should also be understood that it is contemplated that the methods described herein are applicable at least to mammalian, including human, cells.
Stem Cells and REC Progenitors
A methodology to isolate and grow REC from adult human kidneys has been published (Smith et al. (2006), M
Unfortunately, this technology is still in its infancy and does not provide a timely solution to cell sourcing for renal replacement therapy. Researchers have gone to great lengths to ascribe this regeneration potential to hematopoetic or mesenchymal adult stem cells with controversial results (Kale et al. (2003), J. C
Isolation and Amplification of REC Progenitors
Human REC populations are derived from human kidney transplant discards found unsuitable for transplantation due to anatomic or fibrotic defects. Thus, the number of available kidneys is limited and tissues are suboptimal, usually from older donors with indices that preclude kidneys from being suitable for donation. Additionally, organs undergo 24 to 36 hours of cold ischemia (average time range from organ procurement to REC isolation), causing damage to differentiated tubules much like acute tubular necrosis (ATN). Historic REC isolation proceeds as follows: the kidney cortex is removed and mildly digested, slurry is passed through a 600 μm sieve and plated densely onto collagen IV (COLIV) coated/fetal calf serum (FCS) adsorbed dishes. After 24 hours, all non-adherent tissue is removed, and the differentiating factor retinoic acid (RA) is included in the culture media after initial confluence is achieved. By focusing on the isolation of whole tubules and the maintenance of REC in fully differentiated epithelial sheets, this method greatly sacrifices the proliferation potential of isolated cells.
Therefore, methods according to the invention promote precursor expansion to increase REC yield. In certain embodiments, primitive kidney progenitors are amplified to produce a large biomass without contact inhibition, with retinoic acid being added to induce functional differentiation just prior to use. With the historic isolation method, mild digestion and low centrifugal force during washes leads to the isolation of only the healthiest, intact tubules while more buoyant, damaged tubules having high proliferative potential are removed in the supernatant. In accordance with the invention, more complete digestion of cortex tissue disrupts tubule basement membranes and stimulates repopulation, as seen in ATN. For example, as shown in
Dilute plating density and passage ratio to avoid contact inhibition, partial media changes and retention of non-adherent cell populations according to the invention contribute to the prolonged maintenance of the proliferative environment. As shown in
Optionally, the pelleted slurry can be resuspended before plating (or provision to another cell growth surface). If resuspended, the amount of pelleted slurry is adjusted mathematically. For example, if 1 mL total of pelleted slurry is created by the digesting, separating, and washing steps, and it is resuspended in 1 mL of fluid, then 20 μL or less of the resuspended pelleted slurry/cm2 tissue culture plate is used. If 1 mL total of pelleted slurry is created by the digesting, separating, and washing steps, and it is resuspended in 9 mL of fluid, then 100 μL or less of the resuspended pelleted slurry/cm2 tissue culture plate is used.
Subculturing is accomplished by passaging the culture (or subsequent subcultures), typically prior to confluence (confluence is about 1.7×105 cells/cm2 for human RECs), using about 8.7×104 or fewer cells from the culture per square centimeter of surface area on which the subculture is to be grown. This subculture dilution ratio is also referred to herein as passage ratio. It is contemplated that any number of cells from about 8.7×104 or lower can be used. For example, about 8.7×104 or fewer cells can be used, about 8.0×104 or fewer cells can be used, about 7.0×104 or fewer cells can be used, about 6.0×104 or fewer cells can be used, about 5.0×104 or fewer cells can be used, about 4.0×104 or fewer cells can be used, about 3.0×104 or fewer cells can be used, about 2.0×104 or fewer cells can be used about 1.0×104 or fewer cells can be used, about 5.0×103 or fewer cells can be used, about 2.0×103 or fewer cells can be used, and about 1.0×103 or fewer cells can be used can be used. Any of the subculturing ratios shown in Table B can be used as well. Because the passage ratio concept can be expressed in several ways, Table B shows the correspondence between each method of expression. The values in the columns of Table B are calculated as follows. It is estimated, based upon empirical studies, that the number of human renal cells on a confluent 100 mm plate having a surface area of 57.7 cm2 is 107. This number is divided by the dilution factor (e.g., a passage ratio of 1:16 has a dilution factor of 16) to yield the Number of cells to be plated per 100 mm tissue culture plate. The result is the number in the second column of Table B. Then, the value in the second column is divided by 57.7 cm2 to yield the number of cells to be plated per square centimeter of the surface area on which the cells will grow. Knowing this number, even though a culture or subculture may not be confluent, one can determine how much to “dilute” the growing cells on each passage by knowing how many cells to add to the new culture surface. Furthermore, because different cell types may have slightly different cell numbers on a 100 mm tissue culture plate at confluence, the absolute number of cells to be provided for a particular passage ratio may vary slightly. However, once the number of cells at confluence is known, the absolute number of cells to be provided (and, hence, the passage ratio) can be calculated for any cell type. It should also be understood that while plating on tissue culture plates is mainly discussed herein, the passage ratios described herein are relevant to any mode of subculturing where cells grow on a surface. Thus, any growth surface, such as culture flasks and the like, are equally applicable to the concepts described herein.
Historically, RA was used to promote tubule cell differentiation, but since it also reduces the doubling potential of REC precursors, methods according to the invention typically remove RA from cultures and/or subcultures until terminal differentiation just prior to use.
Application of these concepts to porcine REC isolation resulted in greater than a 9.6×106 fold increase in cell mass prior to final senescence. During the expansion phase there was no indication of advanced senescence of the propagating cells or detected decrease in REC purity or therapeutic potential. This increase in biomass can be thought of in terms of the number of devices (e.g., the RAD device) to be manufactured from a kidney isolate. Historical manufacturing practices yield 5-10 devices per donor kidney, with the maximum production of 100,000 RAD annually. In contrast, methods according to the invention for enhanced cell propagation are contemplated to increase manufacturing potential by at least four orders of magnitude, allowing for the construction of 100,000 units per donor kidney or 1,000,000,000 devices annually.
Clinical Evaluation of Efficacy of Renal Cell Therapy
After encouraging pre-clinical animal data, including the use of a porcine SIRS model, the FDA approved a Phase I/II clinical trial to evaluate the safety and efficacy of renal cell therapy utilizing the RAD on ten critically ill patients with ARF and multiorgan failure receiving continuous venovenous hemofiltration (CVVH). The predicted hospital mortality rates for these patients averaged greater than 85%. The devices were seeded with human renal proximal tubule cells isolated from kidneys donated for cadaveric transplantation but found to be unsuitable for transplantation due to anatomic or fibrotic defects. The results of this clinical trial demonstrated that the experimental treatment could be delivered safely under study protocol guidelines for up to 24 hours when used in conjunction with CVVH. The clinical data indicated that the RAD exhibited and maintained viability, durability, and functionality in this clinical setting. Cardiovascular stability of the patients was maintained, and increased native kidney function, as determined by elevated urine outputs, temporally correlated with RAD treatment. The device also demonstrated differentiated metabolic and endocrinologic activity. All but one treated patient with more than a three day follow-up showed improvement, as assessed by acute physiologic scores. Six of the ten treated patients survived past 28 days with kidney function recovery, although mortality rates predicted for these ten patients using the acute physiology, age, chronic health evaluation (APACHE) 3 scoring system were on average 85 percent. Plasma cytokine levels suggest that RAD therapy produces dynamic and individualized responses in patients depending on their unique pathophysiologic conditions.
These favorable Phase I/II trial results led to a subsequent FDA-approved, randomized, controlled, Phase II investigation at 12 clinical sites to determine whether this cell therapy approach alters patient mortality. This Phase II study involved 58 patients, of whom 40 were randomized to RAD therapy and 18 made up a control group with comparable demographics and severity of illness by SOFA scores. The incidence of sepsis in this clinical trial was 73% in the RAD treated group and 67% in the control group. The efficacy of the RAD in patients with sepsis demonstrates the ability of renal cell therapy to reverse sepsis in the clinical setting. The early results were as compelling as the Phase I/II results. Renal cell therapy improved the 28-day mortality rate from 61% in the conventional hemofiltration-treated control group to 34% in the RAD-treated group (Tumlin et al. (2008), J. A
Of note, the RAD clinical trial was halted due in part to manufacturing hurdles that could not be overcome due to resource limitations. These manufacturing difficulties were caused predominantly by the need to maintain the RAD in culture prior to clinical use, demanding significant hands-on technical time. The WEBAK device design allows for the device to be both miniaturized and cryopreserved, mitigating some of the shortcomings of the RAD design.
The invention will now be illustrated by means of the following examples which are given for the purpose of illustration only and without any intention to limit the scope of the present invention.
A pilot study was initiated using a porcine kidney to determine which variables have an effect on REC propagation. The variables tested included enzymatic digestion, particle size, centrifugation, plating density and media changes, plating matrix, subculture conditions, and the use of differentiation factors. In accordance with the invention, more efficient digestion of cortex tissue generated slurry that included precursor cells with enhanced proliferative potential. The slurry was further separated by size, and higher centrifugal force was applied to better retain precursor cells. Other variables such as diluting plating density, partial media changes, subculture conditions (e.g., passage ratio), and the retention of non-adherent cells contributed to the prolonged maintenance of a proliferative environment. It is contemplated that any one, or any combination of two or more, of these variables can be used to improve cell yield in accordance to the invention.
(1) Methods
A 22-pound Hampshire mix pig was anesthetized and intubated with isoflurane. Kidneys were perfused with ice cold saline prior to removal from the animal and the isolation proceeded within one hour of removal. Kidneys were dissected and cortex minced at which time the tissue was split into two equivalent groups and further processed using either the historic method or enhanced propagation (EP) protocol of the invention. A complete description of protocol modifications can be found in Table 1, below. It should be understood that the EP protocol shown in Table 1 is one embodiment of the invention. As shown in the experiments below, variations of the conditions shown in Table 1 also are useful to enhance cell yield.
Briefly, for enhanced propagation, changes included using an optimized enzyme blend (Liberase vs. crude collagenase) and raising centrifugation speed to be inclusive of single cells and damaged (more buoyant) tubules. To determine the effect of particle size on yield, the part of the slurry was sieved into three sub-groups, 212-600 μm, 40-212 μm, and less than 40 μm (single cell). To evaluate plating density, cells were serially diluted by a factor of 2 (1:4, 1:8, 1:16, 1:32, 1:64). To allow the maximum time for progenitor migration onto the plate and avoid the removal of autoregulators that are secreted by cells during this initial outgrowth phase, partial media changes were performed as needed and all non-adherent cells (NAC) retained. To determine whether NAC should continue to be included in REC cultures, NAC from 1:16 at first passage were retained as a separate population and tested for REC characteristics. Cultures were further split by passage ratios of 1:8, 1:16, and 1:32, corresponding to 2,500,000, 1,250,000 and 625,000 cells/20 mL/75 cm2 flask, respectively. For comparison, all yields are calculated as cells per gram kidney cortex.
(2) Cell Yield Comparison
Plating Density:
As shown in
Enzymatic Digestion:
A Liberase Blendzyme was used. Liberase Blendzymes are mixtures of highly purified collagenase and neutral protease enzymes, formulated for efficient, gentle, and reproducible dissociation of tissue from a wide variety of sources. The purified collagenase enzymes are isoforms I and II, as specified by the nomenclature of Bond and Van Wart. The target substrates for these enzyme blends are the collagen and non-collagen proteins that comprise the intercellular matrix. As shown in
Sub-Culture:
Cell Yield:
Finally, the actual cell yield from EP method of the invention compared to the historic method is shown in
(3) Morphology
Cell populations obtained from the more aggressively digested enhanced propagation protocol existed as both an adherent and a non-adherent fraction that either aggregated or expanded to form hollow spheroids (
(4) Tubule Differentiation
The ability to form differentiated tubule structure remains the fundamental characteristic of RECs. After one month of culture, representative EP cell populations were suspended at 106 cells/mL in a three dimensional matrix consisting of Collagens I and IV. Tubulogenesis was stimulated by the addition of 25 ng/mL hepatocyte growth factor (Williams et al. (2003), J. A
(5) Oxidative Stress Response:
γ-glutamyltranspeptidase (γGT) is typically expressed on the apical brush border membrane of proximal tubules and catalyzes the recovery of glutathione. Glutathione deficiency has long been associated with oxidative stress (Curthoys (1983), M
(6) Cytokine Response
Secretion of IL-8, an important mediator in the innate immune system, is stimulated by LPS through toll-like receptors known to be present in epithelial cells. Additionally, basal secretion of IL-8 has been approved by the FDA as a measure of efficacy for release of human RAD used during Phase I/II clinical trials. Therefore, IL-8 secretion, both basal and stimulated by 10 μg/mL LPS challenge, was evaluated using a porcine-specific IL-8 Elisa kit (R&D Systems). Comparison of historic method cell populations and all enhanced propagation cell populations revealed a nearly 10 fold greater IL-8 secretion level for stimulated conditions as compared to basal conditions for both historic and EP population cells. Within the enhanced propagation conditions, altering initial plating density (1:8, 1:16 and 1:32) did not alter IL-8 secretion. Non-adherent cells (NAC) (discarded using historic protocols) and cell populations created from digestion of tissue to sizes under 40 μm (“<40 μm”) were also similar (
(7) Conclusions
Application of enhanced REC propagation techniques to a porcine kidney model yielded more than 732 times more cells than historic propagation methods after only 6 weeks of culture. Previously excluded NAC cell populations retained all REC characteristics tested and may further advance cell yield. Studies indicate that yield increase is due to the stimulation of a transient amplifying precursor pool that persists in the adult kidney. Maintenance of this precursor pool in an undifferentiated state followed by the addition of RA at confluent density resulted in a morphologically correct renal epithelial cell population that retained physiologic therapeutic potential as measured by γGT enzyme activity and IL-8 secretion rates. Historic manufacturing practices yield 5 to 10 devices per kidney isolate, with the maximum production of 100,000 RAD annually. Continuation of this porcine REC enhanced propagation culture, shown in
This Example shows application of enhanced propagation techniques according to the invention to human RECs (HRECs) and the improved outcome of these techniques. More specifically, the Example describes (1) application of the enhanced propagation techniques to increase REC yield from human kidneys; (2) evaluation of the equivalence of HRECs obtained using the historic method and those obtained using enhanced propagation techniques according to the invention; (3) evaluation of the effects of cryopreservation on HREC identity and yield; and (4) integration of cryopreserved HREC obtained using enhanced propagation techniques according to the invention into a Bioartificial Renal Epithelial Cell System (BRECS), a type of WEB AK device.
(1) Parameters to Enhance REC Yield from Human Kidneys
Rationale:
The historic isolation protocol for REC is focused on the maintenance of fully differentiated epithelial sheets and greatly sacrifices the proliferation potential of isolated cells. Derived from intact cortex aggregates up to 600 μm in size, non-adherent particles are aspirated after 24 hours, removing a large REC precursor pool, and the renal tubule cell differentiation factor, retinoic acid (RA), is added at confluence. Propagation of cells cultured in this environment are limited by contact inhibition and does not take advantage of the inverse relationship between differentiation and proliferative potential. Application of enhanced propagation (EP) techniques according to the invention that include disruption of the tubular structure by more efficient enzymatic digestion, decreasing aggregate size, dilute plating ratios (both initial plating density and passage ratio), and removal of the differentiating factor, retinoic acid, from precursor cultures are demonstrated to a dramatic positive effect on cell yields.
Research Plan Overview:
Tissues are highly variable in age, disease state and time from procurement to isolation. Hence, the isolation of REC from at least six donors was chosen to assess the overall proliferative potential of tissues obtained. The isolation schedule consisted of two in the first month and approximately one every five weeks during the first six months of the project. When the effect of cryopreservation was to be tested, cryopreservation of cells at first subculture was performed and was followed by the prolonged expansion of both freshly isolated and reconstituted cryopreserved cells.
Kidney cortex tissue from each of six individual donors were minced and split into two equivalent units and processed using both historical and enhanced propagation protocols. A complete description of the protocols appear in Table 1, and are the same protocols as those used with porcine cells in Example 1. Slurry obtained from the more efficient digestion was further fractionated to establish the desired particle size threshold. At primary plating, fractionated cells were further subdivided to evaluate the effects of plating density and dilute passage ratio on yields. Culture was continued on selected populations showing the greatest potential for expansion. Cell yields from enhanced propagation were compared to yields from historical methods and evaluated in the context of supplying biomass necessary for projected ARF and ESRD therapies. A cell yield range per donor kidney was established that can be used to directly evaluate feasibility of device manufacture. It should be understood that the EP protocol shown in Table 1 is one embodiment of the invention. As shown in the experiments below, variations of the conditions shown in Table 1 also are useful to enhance cell yield.
Tissue Procurement:
Human cadaver kidneys were procured from the National Disease Research Interchange (NDRI). These kidney transplant discards, rejected for organ transplantation due to anatomic or fibrotic defect, were subjected to further screening on an individual basis to exclude unsuitable donors. Tissue was accepted from adults less than 80 years of age that tested negative for adventitious viruses, was not septic, had normal creatinine and blood urea nitrogen levels and no other indices of kidney disease. The ability to incite tissue processing 36 hours post cross clamp also was required. As the procurement agency, NDRI maintained all necessary records regarding donor identification. Tissue was simultaneously processed using historic protocols and using enhanced propagation protocols.
Enzymatic Digestion:
Kidneys were dissected clean of all external tissue and fat; the capsule discarded; and cortex excised from medulla. Cortex was minced with a sharp blade to a fine paste and digested at 1 g/mL in prewarmed collagenase/DNase (0.239 Wunchst units and 250 Kunitz units/mL) at 37° C. for 20 minutes. Using the historical protocol, the digestion was quenched with cold DMEM and passed through an 850 μm sieve. The population more than 850 μm was returned to the digestion flask and cycles were repeated until most tissue was less than 850 μm in size. The resulting slurry was subsequently fractionated over 710 and 600 μm sieves without further enzymatic digestion and remaining tissue aggregates greater than 600 μm were discarded. Cells were washed by centrifugation at 50×(g). Using the enhanced propagation protocol, a more efficient digestion process was accomplished by the substitution of Liberase Blendzyme 1 (available from Roche Applied Science, Germany) for collagenase at an equivalent activity and further disruption of tubular structure by digestion to a much smaller particle size. Liberase Blendzyme 1 is well characterized and has been specifically designed for tissue dissociation and eliminates the need for collagenase lot testing. If Liberase Blendzyme 1 is unavailable, a suitable collagenase IV can be used. The enzymatic digestion was not quenched with cold DMEM between digestion rounds but was kept warm with only the sieved filtrate being quenched as it passes into the collection beaker containing cold DMEM. This allowed the digestion to proceed uninterrupted. Aggregate sizes ranging from 212-150 μm, less than 150 to 90 μm, less than 90 to 38 μm, and less than 38 μm (single cell) were tested and evaluated for effects on cell yield. Cells were washed prior to plating by centrifugation at 300×(g) to create the pelleted slurry, which was inclusive of single cells and cell aggregates.
The effect of particle size and plating density on cell yield is shown in
Primary Plating:
For both the historical and EP methods, initial cell plating density was determined by estimating cell pellet size which inherently had different cell density due to the different g force used for centrifugation. However, for direct comparison, the cell yield was calculated per gram of cortex and per donor kidney. For both methods, cells were cultured in UltraMDCK Media (Lonza) supplemented with ½× the manufacturer's recommended dilution for insulin, transferrin, ethanolamine and selenium (ITES) supplement, 60 ng/L epidermal growth factor (EGF), 10−9 M triiodothyronine (T3) and 1× penicillin-streptomycin. Standard plating density was ½ mL (500 mg) of 50×(g) pellet/12 mL/100 mm plate and non-adherent fraction was aspirated after 24 hours in culture. Initial plating density was evaluated over a wide range by dilution of the 300×(g) pellet from ½3 (1:8) by orders of 2 to ½9 (1:512). To retain inherently expressed growth factors, only 50% of media was changed on an as needed basis. The need for media change was assessed by monitoring glucose, lactate and pH of culture media.
The effect of plating density on cell yield is shown in
Sub-Culture:
For both the historic and EP methods, sub-culture was accomplished as follows. Cells were rinsed free of divalent cations. Subsequently, cells were enzymatically released from plates with 0.05% Trypsin-versene (Lonza) by incubation for 20 minutes at room temperature. Reaction was inhibited by 0.1% soybean trypsin inhibitor (Gibco). Upon reaching tight confluence, retinoic acid (RA) was added to cultures according to historic method 24 hours before passage at 1:2 and tested at the fifth passage. Cultures remained in RA supplemented media for the duration of the study and continued to be passaged at 1:2 while maintaining confluency between 50 and 100%. Enhanced propagation cultures did not receive RA at confluence and were sub-cultured at 105 cells/mL, 12 mL/100 mm plate and passaged when 50-75% confluent, while maintaining confluency between 6.25 and 75%. All non-adherent cells from the media and rinses were retained and included in the sub-culture passage with the enzymatically released adherent cells from the corresponding plates.
As shown in
Cell Yield:
The maximum cell yield from EP methods according to the invention compared to the historic method is shown in
(2) Equivalence of Human REC Obtained by Historic Method and Those Obtained by Enhanced REC Progenitor Propagation Protocols
Rationale:
The characteristics of primary cell cultures can migrate upon continuous subculture. In order to ascertain the continued therapeutic potential of cultures, populations were allowed to differentiate and were then assayed to ascertain cell identity and retention of therapeutic qualities.
Research Plan Overview:
Tissues were equally split and subjected to both historical and enhanced propagation protocols (Table 1). Progenitor populations derived from enhanced propagation were seeded at confluent density and terminally differentiated by both contact inhibition and the addition of retinoic acid. Cells were assayed for the ability to form polarized renal epithelium using antibodies to acetylated tubulin, zona occludens and γ-glutamyltranspeptidase to reveal apical central cilia, tight junctions and a representative brush border enzyme, respectively. Endotoxin stimulation assays assessed retention of cytokine reactivity by measuring basal IL-8 secretion levels and levels post LPS challenge. γGT enzyme assay complemented immunohistochemical results and demonstrated that γGT is functionally active and that cells retain the ability to respond to oxidative stress. Cells were tested minimally at 7 additional doublings (2e7=128× more cells) and 10 additional doublings (2e10=1024× more cells) as compared to populations obtained from historic protocols.
For all tests, cells were passaged and seeded at confluent density, 106 cells/mL/5 cm2. After 2 days in culture, RA was added to media and cells were cultured for an additional 5 days.
Oxidative Stress Response:
For immunotyping and assay for γGT activity, cells were seeded onto 8 well chamber slides while 12 well culture plates were used for testing cytokine response. Qualitative assessments of γGT activity in living cells were determined by the method of Rutenberg (Rutenberg et al. (1968), J. H
As shown in
Cytokine Response:
The evaluation of cytokine response was carried out as follows. Cells were rinsed and refilled with 1 mL growth media supplemented with 0.1% bovine serum albumin both with and without 10 μg/mL lipopolysaccharide (LPS). After 24 hours, supernatants were removed and clarified by centrifugation, and then the concentration of IL-8 was determined using a human specific ELISA kit (Quantikine ELISA kit R&D systems). Cells derived from enhanced propagation techniques were repetitively tested as the cultures progressed, specifically at time points approximating 100×, 250×, 500×, and, if applicable, over 1000× historic yield. Comparisons were made between progressing EP cells while those generated using historic protocols were tested only at fifth passage, the point at which historic populations were integrated into devices for clinical studies.
(3) Evaluation of Effect of Cryopreservation on REC Identity and Yield.
Rationale:
A manufacturing stop point allows for all required biologics testing to be completed prior to the incorporation of inappropriate tissue into a therapeutic biological device. Also, inclusion of a manufacturing stop point allows for continuity of device manufacture even though kidney donor availability is intermittent. Cryostorage of REC precursors provides the required stop point, but has the potential to reduce cell viability and amplification potential. Accordingly, the effect of cryopreservation on REC precursors was evaluated.
Research Plan Overview:
Isolates were cryopreserved on first passage using serum free cryopreservation solutions, consisting of growth media without retinoic acid and 10% DMSO. REC progenitors were subsequently thawed and subjected to matched enhanced propagation culture protocol parameters shown to maximize yields of differentiated REC (Table 1). Cell yield and retention of REC characteristics were evaluated in REC monolayer culture as described above, and compared to data obtained for fresh isolates.
Cryopreservation:
Cells were cryopreserved at first passage after being cultured according to the enhanced propagation methods of the invention (Table 1). Trypsinized cells were mixed at 107 cells/mL with cryoprotectant, placed in a manual, controlled rate freezing apparatus (Mr. Frosty) at −80° C. overnight and then transferred to the vapor phase of liquid nitrogen. Cells remained frozen for at least 2 weeks prior to use in cryopreservation studies. For use, cells were quickly thawed followed by the slow dilution of cryoprotectant and removal of this solution by centrifugation. Cells were plated at a 1:16 dilution according to the EP methods of the invention and were maintained under EP conditions until seeding at confluent density for characterization studies or integration into a biotherapeutic device. Retinoic acid was added after 48 hours post seeding for the characterization studies and 5-7 days post seeding for the biotherapeutic device studies, allowing for maximal coverage prior to differentiation.
Morphology:
Freshly isolated human REC or cryopreserved human REC were propagated using EP methods. Passage of fresh or reconstituted frozen stocks at a confluent density resulted in the formation of a monolayer with typical cobblestone morphology. Characteristics associated with polarized epithelium were evident in the human REC culture by the punctate AT-1 positive staining indicative of centralized cilia and ZO-1 positive staining indicative of epithelial tight junction (
1-Alpha Hydroxylase Expression:
Vitamin D metabolism is an important hormonal function of human REC. Specifically, the mitochondrial enzyme 1-alpha hydroxylase converts the inactive 25-hydroxyvitamin D3 to the active 1,25-dihydroxyvitamin D3 in the kidney. The activity of 1-alpha hydroxylase in cryopreserved human REC cells was confirmed by determining 1-alpha hydroxylase expression in the cells using RT-PCR. Cryopreserved human REC cells were propagated under EP methods of the invention in the presence of parathyroid hormone (PTH), cAMP, or forskolin. PTH and forskolin increase cAMP cellular levels which, in turn, induce 1-alpha hydroxylase expression. cDNA was extracted from the cells, and RT-PCR was performed to determine 1-alpha hydroxylase expression. As illustrated in
(4) Integration of Cryopreserved Human REC Derived from EP Protocols into a Bioartificial Renal Epithelial Cell System (BRECS):
Rationale:
Carbon disks carrying REC constitute the living component of a BRECS (for example, as described in U.S. Patent Publication No. 2009-0081296), a representative type of WEBAK. The ability to fabricate these constructs using cryopreserved human cells derived from EP methods according to the invention demonstrated manufacturing feasibility. Metabolic parameters can be used to determine biomass per disk, which are then be used to estimate the biotherapeutic potential per device and per donor kidney.
Research Plan Overview:
After thawing and expanding (using EP methods according to the invention) previously cryopreserved cells (which were propagated under EP methods according to the invention up to the point of cryopreservation) from separate donors, the resulting cells were seeded onto carbon disks and cultured directly in BRECS units. Parallel monolayer cultures with known surface area, biomass and cell number were seeded. Metabolism was monitored by oxygen and glucose consumption and lactate production. Values calculated from monolayer cultures were then used to extrapolate the cell-covered surface area, biomass and cell number in the disks. At 2.5 weeks, 5 weeks, 9 weeks, and 11 weeks of BRECS culture, human REC containing carbon disks were fixed and coverage was evaluated by histology.
Integration of Advanced EP Populations into a BRECS:
Cryopreserved cells were seeded post recovery onto porous and trabeculated carbon, niobium-coated disks that constitute the REC-containing biotherapeutic component of the BRECS, a representative type of WEBAK. Cells were seeded by static drop-wise loading of a 75 μL cell suspension in growth media containing 106 cells onto a single disk side followed by 1.5 hour incubation at 37° C. Thereafter, the disk was rotated 180°, which was followed by seeding the opposite side of the disk. Up to 20 cell carrying disks can be cultured per BRECS with 250 mL of media in a recirculating perfusion circuit, at perfusion rates between 10-100 mL/minute. For metabolic assessment of disks, parallel monolayer cultures were created for comparison. Retinoic acid was added after cell growth rate had plateaued, typically at 5-7 days, and supernatant samples taken over the life of the cultures. Changes in glucose and lactate concentrations were determined using commercially available colorimetric kits and used to calculate the glucose consumption and lactate production rates. Rates were compared between the monolayer of known biomass and the disk culture system to estimate the biomass retained within the disks. After 2.5 weeks, 5 weeks, 9 weeks, or 11 weeks, disks were fixed with 4% paraformaldehyde permeated with 0.1% TritonX-100, and cells were subsequently labeled with DAPI to evaluate cell coverage.
Cell Viability:
Cryopreserved human REC were reconstituted and seeded onto carbon niobium disks.
The viability and metabolic activity of cryopreserved human REC cells seeded onto carbon niobium disks were also determined by measuring lactate production. Lactate production was measured over a period of about 80 days. As shown in
Cell viability on carbon niobium disks incorporated into BRECS and viability maintained using a peritoneal dialysis perfusion circuit were also determined. Using a sheep model of chronic renal failure, an animal was treated with a BRECS containing human cells after viability had been confirmed using lactate production and glutathione (GSH) metabolism. After the completion of the peritoneal dialysis animal study, one disk was removed from the 20 disk unit for analysis of cell coverage and viability by DAPI. The remaining 19 disks were placed immediately into an in vitro perfusion circuit containing GSH media. A sample was taken 1 hour later to evaluate glutathione metabolism. Before placement in the peritoneal dialysis circuit, the calculated cell number per disk, as evaluated by GSH metabolism rates, was 0.467×106 and after 48 hours it was 0.478×106. The persistence of glutathione metabolism along with the extensive viable cell coverage as revealed by DAPI staining (
Morphology:
Reconstituted human RECs seeded onto carbon niobium disks were assayed for the ability to form polarized renal epithelium using antibodies to acetylated tubulin (AT-1) and zona occludens (ZO-1) to reveal apical central cilia and tight junctions. Aminopeptidase-N(CD13), a brush border enzyme that facilitates Na+ dependent amino acid transport, and γ-glutamyltranspeptidase (γGT), a brush border enzyme that facilitates glutathione metabolism, also were assessed, with antibody staining being prominent along the entire disk surface (
Cell therapy in chronic care situations may benefit from the use of autogenic cells, such that patient's own cells can be sampled, grown, and used in a therapeutic device used to treat that patient. This example demonstrates the feasibility of an autologous device manufactured for chronic applications using cells from a punch biopsy.
Punch Biopsy:
A punch biopsy procedure was used to demonstrate the feasibility of autologous device manufacture for chronic applications using diseased kidney. Kidney tissue biopsies are routinely performed to aid in the diagnosis of renal disease. It follows that if the improved cell yield demonstrated using cells obtained from whole kidney and using EP methods according to the invention can be applied to small biopsies, autologous devices may be manufactured. Typically, a 14-18 gauge biopsy needle is used to remove a 1 cm long core of tissue from the kidney for diagnosis. To mimic this procedure, a glass Pasteur pipet with a 1.2 mm diameter (equivalent to 16 gauge) was plunged through cortex of an excised human kidney transplant discard, removing a core of tissue 1 cm (l)×0.06 cm (r) (translating to 0113 cm3 of tissue or 11.3 mg of tissue). Liberase blendzyme (0.239 Wunchst units/mL) containing 250 Kunitz units/mL DNase was used to treat the tissue at 37° C. for 30 minutes in shaking water bath, as in Table 1. Cells were passed through a 100 μm sieve into ice cold DMEM. The remainder was placed back in a vial, with 5 mL of fresh enzyme added, and returned to another digestion round. Slurry was collected into 50 mL conical tubes and washed by centrifugation at 300×g to generate pelleted slurry.
Each pellet from each digest was put into 20 mL of RA (retinoic acid) free media, which was UltraMDCK Media (Lonza) supplemented with ½× the manufacturer's recommended dilution for insulin, transferrin, ethanolamine and selenium (ITES) supplement, 60 ng/L epidermal growth factor (EGF), 10−9M triiodothyronine (T3) and 1× penicillin-streptomycin. Passage and continued growth proceeded according to the EP method as shown in Table 1, at an initial plating density of 0.15 mg cortex/cm2 culture plate and passage ratios of 1:16, assuming confluent plate conditions (0.15 mg of kidney cortex yielded 0.55 μL of slurry).
Yield calculations through five passages showed that at least one device can be seeded from one 11 mg biopsy after only six weeks of growth, thus feasibility was demonstrated (
As described previously, historic manufacturing practices yield 5 to 10 devices per donor kidney, with the maximum production of 100,000 RAD annually. Application of EP methods resulted in significant enhancement of cell yield using human REC from five individual donors. As indicated in
The entire disclosure of each of the publications, patent documents, and database references referred to herein is incorporated by reference in its entirety for all purposes to the same extent as if each individual source were individually denoted as being incorporated by reference.
The invention may be embodied in other specific forms without departing form the spirit or essential characteristics thereof. The foregoing embodiments are therefore to be considered in all respects illustrative rather than limiting on the invention described herein. Scope of the invention is thus indicated by the appended claims rather than by the foregoing description, and all changes that come within the meaning and range of equivalency of the claims are intended to be embraced therein.
This application claims the benefit of and priority to U.S. Patent Application Ser. No. 61/073,745, filed Jun. 18, 2008, the entire disclosure of which is incorporated by reference herein.
This invention was sponsored, in part, by the U.S. Army Medical Research and Materiel Command, contract number W81XWH-05-2-0010 and by the SBIR program of the National Institutes of Health, Grant NIDDK R43DK082050. The United States government has certain rights to this invention.
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| Number | Date | Country | |
|---|---|---|---|
| 20100136687 A1 | Jun 2010 | US |
| Number | Date | Country | |
|---|---|---|---|
| 61073745 | Jun 2008 | US |
