Claims
- 1. A method for transforming a target plant cell comprising stably transforming the target cell with a growth stimulation polynucleotide to produce a modified cell, growing the modified cell through at least one cell division to produce a progeny cell expressing the growth stimulation polynucleotide, and then transforming the progeny cell with at least one polynucleotide of interest, wherein the growth stimulation polynucleotide is an anti-apoptosis polynucleotide other than baculovirus p35 or baculovirus iap; a hormone polynucleotide; or a silencing construct targeted against cell cycle repressors.
- 2. The method of claim 1 wherein transformation frequency is increased compared to corresponding plant cells that do not contain the growth stimulation polynucleotide.
- 3. The method of claim 1 wherein the progeny cell is from T0 transgenic cultures, regenerated plants or any subsequent progeny cell expressing the growth stimulation polynucleotide.
- 4. The method of claim 3 wherein the progeny cell is from a T0 regenerated plant or a plant from any subsequent generation expressing the growth stimulation polynucleotide.
- 5. The method of claim 1 wherein the growth stimulation polynucleotide is an anti-apoptosis polynucleotide other than baculovirus p35 or baculovirus iap.
- 6. The method of claim 1 wherein the growth stimulation polynucleotide is a hormone polynucleotide.
- 7. The method of claim 1 wherein the growth stimulation polynucleotide is a silencing construct targeted against cell cycle repressors.
- 8. The method of claim 1 wherein the target cell is from a monocot or a dicot plant.
- 9. The method of claim 8 wherein the target cell is from a monocot.
- 10. The method of claim 9 wherein the target cell is from a maize plant.
- 11. The method of claim 8 wherein the target cell is from a dicot.
- 12. The method of claim 11 wherein the target cell is from a soybean plant.
- 13. The method of claim 8 wherein the target cell is from corn, soybean, sunflower, sorghum, canola, wheat, alfalfa, cotton, rice, barley, potato, tomato, or millet.
- 14. A method of transformation comprising transforming a target plant cell with at least one polynucleotide of interest, wherein the target cell has been previously stably transformed to express a growth stimulation polynucleotide and has gone through at least one cell division, wherein the growth stimulation polynucleotide is an anti-apoptosis polynucleotide other than baculovirus p35 or baculovirus iap; a hormone polynucleotide; or a silencing construct targeted against cell cycle repressors.
- 15. The method of claim 14 wherein the target cell is from T0 transgenic cultures, regenerated plants or any subsequent progeny expressing the transgenic growth stimulation polynucleotide.
- 16. The method of claim 15 wherein the target cell is from a T0 regenerated plant or any subsequent progeny expressing the transgenic growth stimulation polynucleotide.
- 17. The method of claim 14 wherein the growth stimulation polynucleotide is an anti-apoptosis polynucleotide other than baculovirus p35 or baculovirus iap.
- 18. The method of claim 14 wherein the growth stimulation polynucleotide is a hormone polynucleotide.
- 19. The method of claim 14 wherein the growth stimulation polynucleotide is a silencing construct targeted against cell cycle repressors.
- 20. The method of claim 14 wherein the target cell is from a monocot or a dicot plant.
- 21. The method of claim 20 wherein the target cell is from a monocot.
- 22. The method of claim 21 wherein the target cell is from a maize plant.
- 23. The method of claim 20 wherein the target cell is from a dicot.
- 24. The method of claim 23 wherein the target cell is from a soybean plant.
- 25. The method of claim 20 wherein the target cell is from corn, soybean, sunflower, sorghum, canola, wheat, alfalfa, cotton, rice, barley, potato, tomato, or millet.
CROSS REFERENCE TO RELATED APPLICATIONS
[0001] This application is a continuation of co-pending application U.S. Ser. No. 09/613,094 filed Jul. 10, 2000.
Continuations (1)
|
Number |
Date |
Country |
Parent |
09613094 |
Jul 2000 |
US |
Child |
10336980 |
Jan 2003 |
US |