The present invention relates to methods for forming organic molecules, and more particularly, to methods for forming organic molecules comprising contacting a hydrolase enzyme with an organic reactant.
The biomolecular mechanisms of numerous organic molecules are essentially undefined. Particularly, the organic molecules containing silicon are essential for growth and biological function in a variety of plant, animal, and microbial systems, however the molecular mechanism for these interactions are effectively unknown. The in vitro studies of natural systems within the area of silica biosynthesis are complicated. Early mechanistic queries including biomimetic approaches often failed to recognize the chemistry of silicic acid and its analogues. For example, silicatein was determined to catalyze the polycondensation of tetraethoxysilane during the formation of particulate silica. However, given the limitations of the product and the resultant analysis, the study was not able to differentiate between the role of silicatein in the hydrolysis and condensation reactions during biosilicification.
Thus, there remains a need for improved methods of forming structurally defined organic molecules and materials, particularly organosilicon molecules.
Accordingly, the present invention is directed to methods of forming an organic molecule. The method comprises contacting a hydrolase enzyme with an organic reactant. The organic reactant comprises the formula:
wherein X is selected from the group consisting of silicon and germanium; R1 is selected from the group consisting of alkyl, haloalkyl, unsaturated alkyl, aryl, alcohol, epoxy, ether, amine, —(OXR42)y—OXR43 and a combination thereof; R2 is selected from the group consisting of alkyl, hydrogen, ether and a combination thereof; R3 is selected from the group consisting of alkyl, unsaturated alkyl, aryl, hydrogen and a combination thereof; R4 is selected from the group consisting of alkyl, haloalkyl, unsaturated alkyl, aryl, hydrogen, hydroxy, alkoxy, alcohol, epoxy, ether, amine, —(OXR42)y—OXR43 and a combination thereof; n is an integer from 0 to 4; y is 0 or is an integer greater than 0; and z is 3 or an integer greater than 3. The hydrolase enzyme comprises lipase, protease, phosphoesterase, esterase, cutinase or a combination thereof. The hydrolase enzyme catalyzes the hydrolysis and condensation of the organic reactant to form the organic molecule.
In another embodiment of the invention, a method of forming an organosilicon molecule is provided. The method comprises contacting a hydrolase enzyme with an organosilicon reactant. The organosilicon reactant comprises the formula:
wherein: R1 is selected from the group consisting of alkyl, haloalkyl, unsaturated alkyl, aryl, alcohol, epoxy, ether, amine, —(OSiR42)y—OSiR43, and a combination thereof; R2 is selected from the group consisting of alkyl, hydrogen, ether and a combination thereof; R3 is selected from the group consisting of alkyl, unsaturated alkyl, aryl, hydrogen and a combination thereof; R4 is selected from the group consisting of alkyl, haloalkyl, unsaturated alkyl, aryl, hydrogen, hydroxy, alkoxy, alcohol, epoxy, ether, amine, —(OSiR42)y—OSiR43 and a combination thereof; n is an integer from 0 to 4; y is 0 or an integer greater than 0; and z is 3 or is an integer greater than 3. The hydrolase enzyme comprises lipase, protease, phosphoesterase, esterase, cutinase or a combination thereof. The hydrolase enzyme catalyzes the hydrolysis and condensation of the organosilicon reactant to form the organosilicon molecule.
In yet another embodiment of the present invention, a method of forming an organosilicon intermediate molecule is provided. The method comprises contacting a hydrolase enzyme with an organosilicon reactant. The organosilicon reactant comprises the formula:
wherein: R1 is selected from the group consisting of alkyl, haloalkyl, unsaturated alkyl, aryl, alcohol, epoxy, ether, amine, —(OSiR42)y—OSiR43, and a combination thereof; R2 is selected from the group consisting of alkyl, hydrogen, ether and a combination thereof; R3 is selected from the group consisting of alkyl, unsaturated alkyl, aryl, hydrogen and a combination thereof; R4 is selected from the group consisting of alkyl, haloalkyl, unsaturated alkyl, aryl, hydrogen, hydroxy, alkoxy, alcohol, epoxy, ether, amine, —(OSiR42)y—OSiR43 and a combination thereof; n is an integer from 0 to 4; y is 0 or is an integer greater than 0; and z is 3 or is an integer greater than 3. The hydrolase enzyme comprises lipase, protease, phosphoesterase, esterase, cutinase or a combination thereof. The hydrolase enzyme catalyzes the hydrolysis of the organosilicon reactant to form the organosilicon intermediate molecule.
In yet another embodiment of the present invention a method of forming an organosilicon molecule is provided. The method comprises contacting a hydrolase enzyme with an organosilicon intermediate reactant. The organosilicon intermediate reactant comprises the formula:
wherein R1 is selected from the group consisting of alkyl, haloalkyl, unsaturated alkyl, aryl, alcohol, epoxy, ether, amine, —(OSiR42)y—OSiR43, and combination thereof; R2 is a hydrogen; R4 is selected from the group consisting of alkyl, haloalkyl, unsaturated alkyl, aryl, hydrogen, hydroxy, alkoxy, alcohol, epoxy, ether, amine, —(OSiR42)y—OSiR43 and a combination thereof; n is an integer from 0 to 4; and y is 0 or is an integer greater than 0; a+b equals z; and z is 3 or is an integer greater than 3. The hydrolase enzyme comprises lipase, protease, phosphoesterase, esterase, cutinase or a combination thereof. The hydrolase enzyme catalyzes the condensation of the organosilicon intermediate reactant to form the organosilicon molecule.
The ability to synthesize organic molecules under the presently defined reaction conditions is advantageous because the reaction conditions assist in hydrolysis and condensation of the organic reactant to form the organic molecule. Other advantages of the present invention will become apparent to those skilled in the art from the following detailed description where alternative exemplary embodiments of this invention are shown and described. As will be realized, the invention is capable of other different, obvious aspects and embodiments, all without departing from the invention. Accordingly, the drawing and descriptions should be regarded as illustrative in nature and not as restrictive.
The study of hydrolysis and condensation reactions in forming organic molecules is complicated. Specifically, such reactions are complicated in biosilicification due to the enhanced sensitivity of silica, silicates, and silicic acid to pH, concentration and temperature. Accordingly, the present invention overcomes such complications by utilizing a hydrolase enzyme to form structurally defined organic and more specifically organosilicon molecules. Specifically, as shown in the following reaction sequences, the hydrolase enzyme non-specifically promotes the hydrolysis of the organic reactant (A) to form an organic intermediate reactant (B) and subsequently selectively catalyzes the condensation of the organic intermediate reactant (B) to form the organic molecule (C).
The organic reactants (A) of Reactions (I), (II) and (III) are acceptable substrates for the hydrolase enzyme to selectively catalyze hydrolysis and condensation of the organic reactant. The reactive sites of the organic reactant (A) comprise an electropositive atom (e.g. silicon or germanium) which facilitates the hydrolysis and condensation of the organic reactant (A) to ultimately form the organic molecule (C). Thus, structurally defined organic molecules may be formed using the organic reactants (A) of Reactions (I), (II) and (III).
One skilled in the art will appreciate the various inorganic elements X which may be utilized in the reactant, any of which may be employed herein. In one embodiment, X is selected from the group consisting of silicon and germanium. In addition, one skilled in the art will appreciate the various substituents which may be utilized for R1, R2, R3, and R4 in the above reactions, any of which may be employed herein. As such, it will be understood by those skilled in the art that the R groups of the organic structures do not have to be identical repeating units. Rather, the R groups may be independently chosen for each of the repeating units.
In one embodiment, R1 is selected from the group consisting of alkyl, haloalkyl, unsaturated alkyl, aryl, alcohol, epoxy, ether, amine, —(OXR42)y—OXR43, and a combination thereof, wherein R4 is selected from the group consisting of alkyl, haloalkyl, unsaturated alkyl, aryl, hydrogen, hydroxy, alkoxy, alcohol, epoxy, ether, amine, —OXR42)y—OXR43 and a combination thereof. In another embodiment, R2 is selected from the group consisting of alkyl, hydrogen, ether and a combination thereof. In yet another embodiment, R3 is selected from the group consisting of alkyl, unsaturated alkyl, aryl and hydrogen. In each embodiment detailed above, it will be further understood that alkyl, haloalkyl, unsaturated alkyl, and alkoxy may be substituents having one carbon or more than one carbon. Moreover, in the above reaction, n is defined as an integer from 0 to 4; y is defined as 0 or as an integer greater than 0; and z is 3 or an integer greater than 3. In addition, * as used throughout the application is indicative of a cyclic architecture, i.e., there is no defined end group.
The organic reactant (A) may be monofunctional or polyfunctional. For example, the formula of the organic reactant (A) may be selected from the group consisting of (R1)4X, (R1)3X(OR2)1, (R1)2X(OR2)2, (R1)X(OR2)3 and X(OR2)4. Specific examples of di-functional organic reactants include, but are not limited to, (CH3)2Si(OCH3)2, (CH3)(CF3CH2CH2)Si(OCH3)2, (C(H5)(CH3)Si(OCH3)2, and (CH3CH2)2Ge(OCH2CH3)2. Specific examples of tri- and tetra-functional organic reactants include, but are not limited to, (CH3)Si(OCH2CH3)3 and Si(OCH2CH3)4, respectively. The organic reactant may also be linear, branched, resinous, or cyclic. In one embodiment, the distributions of linear, cyclic, and branched organic molecules may be filly hydroxylated after the initial interaction with the hydrolase enzyme. Specific examples of cyclic, linear, and branched organic reactants include, but are not limited to, 1,3,5,7-tetramethyl-1,3,5,7-tetramethoxy-cyclotetrasiloxane, 1,3-bis(hydroxy)tetramethyldisiloxane, and [(HO)2(CH3)SiO]3SiCH3, respectively.
Once a suitable organic reactant is identified, the organic reactant is contacted with a hydrolase enzyme in order to catalyze the formation of an organic molecule. The hydrolase enzyme may be derived from a bacterial, fungal, or mammalian source, or the hydrolase enzyme may be derived from any other suitable source. The enzyme is generally present as either a soluble solution or a heterogeneous suspension, and the enzyme may be lyophilized or immobilized. In a particular embodiment, the hydrolase enzyme is selected from the group consisting of lipase, protease, phosphoesterase, esterase, cutinase and a combination thereof. In another embodiment, the hydrolase enzyme comprises a lipase enzyme such as Candida antarctica lipase, Candida antarctica lipase B, Rhizomucor miehei lipase, wheat germ lipase or a combination thereof. In another embodiment, the hydrolase enzyme comprises a protease enzyme such as trypsin, papain, pepsin or a combination thereof.
In yet another embodiment, the hydrolase enzyme is trypsin or an enzyme that is at least 70% homologous with trypsin. Trypsinogen (
Notably, the activation reactions and structural changes cause negligible alterations to the region of the catalytic triad (i.e. Ser183-His46-Asp177,
Unless otherwise indicated, all numbers expressing quantities of ingredients, properties such as molecular weight, reaction conditions, and so forth as used in the specification and claims are to be understood as being modified in all instances by the term “about.” Accordingly, unless otherwise indicated, the numerical properties set forth in the following specification and claims are approximations that may vary depending on the desired properties sought to be obtained in embodiments of the present invention. Notwithstanding that the numerical ranges and parameters setting forth the broad scope of the invention are approximations, the numerical values set forth in the specific examples are reported as precisely as possible. Any numerical values, however, inherently contain certain errors necessarily resulting from error found in their respective measurements.
The concentration, temperature and pH of the reaction may be varied as set forth in detail below. Specifically, the concentration of the hydrolase enzyme is generally greater than 1 mg/mL. In another embodiment, the concentration of the hydrolase enzyme is from about 10 mg/mL to about 80 mg/mL. In yet another embodiment, the concentration of the hydrolase enzyme is from about 20 mg/mL to about 60 mg/mL. In yet another embodiment, the concentration of the hydrolase enzyme is about 40 mg/mL. In one embodiment, the organic reactant to enzyme mole ratio is less than or equal to about 40000:1. In another embodiment, the organic reactant to enzyme mole ratio is less than or equal to about 1000:1.
The temperature of the reactions is generally between about 5° C. and 90° C. In another embodiment, the reaction is carried out at a temperature of between about 20° C. to about 50° C. In yet another embodiment, the reaction is carried out at a temperature about 25° C. The pH of the reaction is generally from about 5.0 to about 8.0. In one embodiment, the pH of the reaction is about 7.0.
In addition, the reactions may be performed under solventless (neat) conditions, or the reactions may be performed utilizing an aqueous solution or a solvent. Suitable solvents include, but are not limited to, water miscible organic solvent such as THF and acetonitrile, and relatively dry organic solvents such as toluene and hexane.
In order that the invention may be more readily understood, reference is made to the following examples, which are intended to be illustrative of the invention, but are not intended to be limiting in scope.
Materials
Protein and Peptides
Aspergillus niger lipase (Amano lipase A, #53,478-1), N-α-benzoyl-L-arginine ethyl ester (BAEE, #B4500), N-benzoyl-L-tyrosine ethyl ester (BTEE, #B6125), bovine pancreatic α-chymotrypsin (#C-4129), bovine pancreatic α-chymotrypsin treated with TLCK (#C3142), bovine pancreatic phospholipase A2 (P-8913), bovine pancreatic trypsin (#T4665), bovine pancreatic trypsin treated with TPCK (#T1426), bovine serum albumin (BSA, #B4287), Candida antarctica lipase (#62299), Candida lipolytica lipase (#62303), Gadus morhua trypsin (#T9906), hog stomach pepsin (#P7012), Mucor javanicus lipase (#62304), Novozyme 435® (immobilized Candida antarctica lipase B, ˜1% protein, #53,732-2), papaya latex papain (#P4762), Penicillium roqueforti lipase (#62308), porcine β-globulins (#G2512), porcine pancreatic carboxypeptidase B (#C9584), porcine pancreatic elastase (#E0258), porcine pancreatic lipase (#L-3126), porcine pancreatic trypsin (#T0303), protease (subtilisin Carlsberg, #P8038), Pseudomonas cepacia lipase (Amano lipase PS, #53,464-1), Pseudomonas fluorescens lipase (#62321), Rhizomucor miehei lipase (#62291), trypsin inhibitor from Glycine max (Popcorn inhibitor from soybean, #T9128), trypsin-chymotrypsin inhibitor from soybean (Bowman-Birk inhibitor, #T9777), N-α-p-tosyl-L-lysine chloromethyl. ketone hydrochloride,(TLCK, #T7254), N-tosyl-L-phenylalanine chloromethyl ketone (TPCK, #T4376), wheat germ lipase (#62306) were purchased from Sigma-Aldrich (St. Louis, Mo.). Bovine kidney cathepsin L (#219418), human liver cathepsin L (#219402), and Paramecium tetraurelia cathepsin L (#219412) were purchased from Calbiochem®, EMD Biosciences (San Diego, Calif.). Recombinant bovine trypsin expressed in maize (#TRY, CAS #9002-07-7) was purchased from ProdiGene (College Station, Tex.).
Water
Ultra pure water was obtained from a Milli-Q system at the Dow Corning Corporation (Midland, Mich.). Trizma® pre-set crystals pH 7.0 (Tris-HCl, #T3503), pH 7.5 (Tris-HCl, #T4128), pH 7.8 (Tris-HCl, #T4503), pH 8.0 (Tris-HCl, #T4753), and pH 9.0 (Tris-HCl, #T6003) were purchased from Sigma-Aldrich. Buffer solutions pH 4.00 (potassium biphthalate, #SB101), pH 5 (sodium hydroxide-citric acid, #A015860101), pH 6.00 (monobasic potassium phosphate-sodium hydroxide, #SB104), and pH 10.00 (potassium carbonate-potassium borate-potassium hydroxide, #SB1 15) were purchased at Fisher Scientific (Pittsburgh, Pa.).
Organic and Inorganic Molecules
Acetonitrile (#A996-4), acetone (#A929-4), phosphorous pentoxide (#A244), 2-propanol (#A451-4), tetrahydrofuran (#T427-1), and toluene (#T291-4) were purchased from Fisher Scientific. Acetic acid (#A6283), calcium chloride (#C3881), dodecane (#44010), ethanol (#45,984-4), hexanol (#H13303), hydrochloric acid (#33,925-3), lithium aluminum hydride (#19,987-7), sodium chloride (#20,443-9), and sodium hydrogencarbonate (#34,094-4) were purchased from Sigma-Aldrich. Tetraethylene glycol monomethyl ether (#T1372) was purchased from TCI America (Portland, Oreg.). HPLC grade organic solvents were used throughout the examples
Silicon-Based Molecules
Trimethylsilanol (CAS #1066-40-6, #12848-72), heptamethylhydroxytetracyclosiloxane (#11050-134B), hexamethylcyclotrisiloxane (#E-459-80, cut #3), octamethylcyclotetrasiloxane (#E-1927-93-2, lot #2), 1,3,5-trimethyl-1,3,5-tri(3,3,3-trifluoropropyl)cyclotrisiloxane (LS Trimer), 1,3,5,7-tetramethyl-1,3,5,7-tetra(3,3,3-trifluoropropyl)cyclotetrasiloxane (#H-1387-145), and 2,4,6,8-trimethyl-2,4,6,8-tetraphenyl-cyclotetrasiloxane (#1923-44A) were obtained at the Dow Coming Corporation. 3-Aminopropyldimethylethoxysilane (#SIA0603.0), bis(trimethylsilyl)acetamide (#SIB 1846.0), 1,1-dimethyl-1-sila-2-oxacyclohexane (#SID4234.0), 3-glycidoxypropyldimethylethoxysilane (#SIG5825), hexamethyldisiloxane (#SIH6115.0), trimethylethoxysilane (#SIT8515.0), and triphenylethoxysilane (#SIT8652.0) were purchased at Gelest, Inc. (Tullytown, Pa.). Hexamethyldisilazane (#37921-2) and tetraethoxysilane (#23620-9) were purchased at Sigma-Aldrich. Phenyldimethylethoxysilane (#P0161) was purchased from United Chemical Technologies, Inc. (Bristol, Pa.). Methyltriethoxysilane (#M9050) was purchased from Huls America, Inc. (Bristol, Pa.).
Synthesis of Trimethylalkoxysilane
Two trimethylalkoxysilanes were synthesized with polar and non-polar leaving groups. Tetraethylene glycol monomethyl ether (TGME) and hexanol were silylated with bis(trimethylsilyl)acetamide to obtain the target polar (Me3SiO(CH2CH2O)4CH3) and non-polar (Me3SiOC6H13) silanes, respectively.
Biocatalysis Study
This study was directed to a quantitative test of the ability of enzymes to catalyze the formation of siloxane bonds during the in vitro hydrolysis and condensation of alkoxysilanes. Since mono-functional silanes were chosen as reactants during the formation of molecules with a single siloxane bond, rigorous procedures were established to prepare glassware, as well as isolate and quantitatively analyze the reaction products by gas chromatography (GC).
The measure of extraction efficiency was defined as the percent yield of reactants and products. The mass balances were equal to the sum total of the extraction values. Based on the chromatographic results, the extraction efficiencies of THF at 25° C. were excellent in comparison to diethylether, methylene chloride and toluene. Based on two extractions, mass balances greater than 98% were routinely obtained by THF.
Based on triplicate measurements, the response factors for the analytes were calculated and determined to be linear as a function of concentration over three orders of magnitude (i.e. 0.1-10% (w/w)). The alkoxysilane, silanol, and disiloxane analytes were chromatographically resolved as illustrated in
Enzyme-Catalyzed Condensation Study
This study was directed to an enzyme screen with trimethylsilanol in order to evaluate the ability of various enzymes to catalyze the formation of a siloxane bond. Based on the similarities of the proteins isolated from the Tethya aurantia marine sponge (silicatein), Cylindrotheca fusiformis diatom (silaffin), and Equisetum telmateia plant (biopolymer extract), a series of lipases and serine-proteases were selected as homologous proteolytic enzymes. In addition to catalyzing comparable reactions (i.e. the hydrolysis of amide and/or ester bonds), the active sites in the hydrolase enzymes are composed of similar serine-histidine-aspartate catalytic triads. In comparison to control reactions, the mammalian, fungal, and bacterial lipases and proteases detailed in Table 1 were screened with trimethylsilanol. In this study, control reactions were defined as non-enzymatic reactions. Specifically, experiments conducted in the absence of a protein were defined as negative control reactions. Proteinaceous molecules such as bovine serum albumin and porcine γ-globulins were used to study non-specific protein catalysis.
1Candida antarctica lipase B was immobilized on acrylic resin beads (Novozyme ® 435).
Prior to reaction, the glass vials were rinsed with acetone (x2) and ethanol (x2), dried, and silylated with hexamethyldisilazane (1 mmol) in the presence of acetic acid (0.2 mmol) for 30 m at 25° C. Subsequently, the vials were rinsed with ethanol (x2) and dried in an oven at 110 ° C. The silylated glass vials were confirmed not to contaminate the reactions with trimethylsilanol or hexamethyldisiloxane.
The reactions were formulated with a 5:1 trimethylsilanol (225 mg) to protein (45 mg lipase, protease, or BSA) weight ratio in 1.3 mL toluene or 1.1 mL water. Toluene was dried over lithium aluminum hydride (‘dry toluene’, 11 ppm water) and hydrated with Milli-Q water (‘wet toluene’, 467 ppm water). Karl Fischer titrations were performed on an Aquatest IV titrator (Photovolt Corporation, New York, N.Y.) to measure the water content in toluene. Milli-Q water (‘water’) was buffered with 50 mM Tris-HCl buffer, pH 7.0 (‘buffered pH 7’). The estimated solubility of trimethylsilanol in water is 4.2% (i.e. 42.56 mg/mL). Based on the formulation, the two-phase reactions conducted in water were saturated with trimethylsilanol (˜200 mg/mL). The closed (screw capped) reactions were conducted in inert glass vials at 25° C. with magnetic stirring for 6 days. The reaction products were isolated and quantitatively analyzed by GC. Prior to analysis, the aqueous reactions were extracted (x2) with THF in the presence of NaCl and filtered through a Whatman Autovial® 5 0.45 μm Teflon® filter (#AV115NPUORG).
The results are summarised in Table 2. In comparison to negative control and non-specific protein (i.e. BSA) reactions, select lipases as well as trypsin and α-chymotrypsin were observed to catalyse the condensation of trimethylsilanol during the formation of hexamethyldisiloxane under mild conditions. The enzymes checked (✓) in Table 2 were determined to catalyse the condensation of greater than ten times more trimethylsilanol than the negative control reactions; as well as, greater than three or ten times more trimethylsilanol than the BSA reactions in organic and aqueous media, respectively. Conversely, the ability of the unchecked enzymes in Table 2 to catalyse the model condensation reaction was not substantially different than the control reactions. In review, the relative rate of condensation increased in water. As opposed to lipases, proteases will only interact with water soluble substrates. The estimated water solubility of trimethylsilanol is 4.2% (i.e. 42.56 mg/mL).
1Candida antarctica lipase B was immobilized on acrylic resin beads (Novozyme ® 435).
Protease-Catalyzed Silanol Reactions
Based on the exceptional activity of trypsin and α-chymotrypsin from bovine pancreas (Table 2), protease enzymes were identified as target catalysts. Consequently, a series of serine-, cysteine-, aspartic-, and metallo-proteases were selected in order to evaluate their ability to catalyze the formation of a molecule with a siloxane bond in a neutral medium (pH 7.0) (Scheme 1).
In comparison to control reactions, the ability of the proteases detailed in Table 3 to catalyse siloxane condensation was screened with trimethylsilanol. Comparatively, the original reactions conducted in the enzyme-catalysed condensation study were performed for six days versus three hours in this study.
Prior to reaction, the glass vials were silylated. The reactions were formulated with a 4:1 trimethylsilanol (80 mg) to protein (20 mg protease, BSA, or 7-globulins) weight ratio (˜1000:l silanol to protease mole ratio) in 0.5 mL of 50 mM Tris-HCl buffered Milli-Q water, pH 7.0.
Based on the estimated solubility of trimethylsilanol in water (42.56 mg/mL), the concentration of trimethylsilanol (˜160 mg/mL) saturated the aqueous media and created two-phase reaction mixtures. The reaction products were isolated and quantitatively analyzed by GC as illustrated in
Trypsin and α-chymotrypsin preferentially catalyzed the condensation of trimethylsilanol under mild conditions. A neutral medium (pH 7.0) was used to differentiate enzymatic vs. chemical catalysis. Given the specificity in the catalytic regions, the proteases are substrate selective. Trimethylsilanol was chosen as a model silanol to study the role of an enzyme in the formation of molecules with a single siloxane bond. Notably, three sources of cathepsin L did not catalyze the condensation reaction. In contrast, although silicatein was documented to be highly homologous with cathepsin L, silicatein catalyzed the formation of particulate silica and silsesquioxanes. The activities of the enzymes depend upon the functionality of the non-natural organosilicon substrates.
Substantial condensation of trimethylsilanol was not observed in the negative control, non-specific protein (i.e. BSA, γ-globulins), small molecule (i.e. CaCl2, imidazole, N-methylimidazole), and polypeptide (i.e. poly-L-lysine) reactions in comparison to the raw material (
Impurity Study
Given an enhanced preference for basic residues such as arginine and lysine, N-α-p-tosyl-L-lysine chloromethyl ketone hydrochloride selectively and irreversibly inhibits trypsin activity without affecting α-chymotrypsin activity. TLCK inhibits trypsin by alkylating the histidine residue in the catalytic triad. After treating α-chymotrypsin with TLCK, the product yield significantly decreased in the replicate condensation experiment
Based on the product yield of the TLCK control reaction (
Trypsin-Catalysed Condensation of Silanols
Prior to the reactions, the glass vials were silylated. The reaction products were isolated and quantitatively analysed by GC. Prior to analysis, the aqueous reactions were extracted (x2) with THF in the presence of NaCl and filtered through a Whatman Autovial® 5 0.45 μm Teflon® filter. In these studies, control reactions are defined as non-enzymatic reactions. Specifically, experiments conducted in the absence of a protein are defined as negative control reactions.
Proteinaceous Inhibition Study
A proteinaceous inhibition study was conducted to investigate the role of the enzymatic active site in the model silanol condensation reaction. Specifically, trypsin and α-chymotrypsin were inhibited with two distinctly different natural polypeptide inhibitors from soybean: a trypsin-chymotrypsin inhibitor (i.e. the Bowman-Birk Inhibitor, BBI) and a trypsin inhibitor from Glycine max (i.e. the Kunitz soybean trypsin inhibitor or Popcorn inhibitor, PCI).
The soybean inhibitors are highly stable proteins with well-defined inhibitory sites. Although the proteinaceous inhibitors originate from the same source, the amino acid sequences, tertiary structures, and properties of the polypeptides are different. The BBI and PCI proteins contain 71 (MW=7,975) and 181 (MW=21,700) amino acids, respectively. BBI contains dual independent regions that selectively inhibit trypsin and chymotrypsin. The reactive sites within these regions are defined as Lys16-Ser17 (trypsin) and Leu43-Ser44 (chymotrypsin). The kinetics and equilibria of the inhibition reactions are independent. PCI selectively inhibits trypsin through interactions with an Arg63-Ile64 reactive site.
Prior to reaction, the enzymes were inhibited with excess inhibitor (i.e. >1:1 (w/w) or 4:1 BBI to protease and 2:1 PCI to protease mole ratios, respectively) in stirred neutral media (pH 7.0) for two hours before the addition of trimethylsilanol. The reactions were formulated with a 4:1 monomer to enzyme weight ratio (˜1000:1 silanol to protease mole ratio) and conducted at 25° C. for three hours. The reaction products were isolated and quantitatively analyzed by GC (
Based on standard enzymatic activity assays, trypsin was fully inhibited with BBI (98%) and PCI (91%), while α-chymotrypsin was partially inhibited with BBI (63%). In comparison to the control reactions, the proteinaceous inhibitors completely inhibited the protease-catalyzed condensation reactions. Since α-chymotrypsin was inhibited by PCI, the presence of trypsin in α-chymotrypsin was confirmed. In addition, condensation was not observed despite the partial inhibition of α-chymotrypsin with BBI. While not wishing to be bound by theory, it appears that the tertiary structure, functionality of the active site, and catalytic triad of trypsin are directly involved in the in vitro condensation of trimethylsilanol.
Temperature Study
The effect of temperature on trypsin as well as the enzyme-catalyzed condensation reaction was studied. Prior to reaction, buffered aqueous solutions (pH 7.0) of trypsin were stirred and equilibrated at set temperatures in a thermostated water bath (±0.1° C.) for 20 minutes. The reactions were formulated with a 4:1 monomer to enzyme weight ratio (˜1000:1 silanol to trypsin mole ratio) and conducted at set temperatures for three hours. In the thermal denaturation experiments, trypsin was boiled for 20 minutes and three hours in 50 mM Tris-HCl buffered Milli-Q water (pH 7.0), independently, before performing the condensation reaction at 25° C. The reaction products were isolated and quantitatively analyzed by GC (
In comparison to the control reactions, trypsin appears to be catalytically active over a broad temperature range. The optimum temperature of the reaction was approximately 25° C. After boiling the solutions of trypsin (40 mg/mL) for different periods of time (i.e. 20 minutes vs. 3 hours), the rates of the trypsin-catalyzed condensation reactions decreased due to the degree of thermal denaturation. Although enzymatic reaction rates may increase with temperature, elevated temperatures have been reported to reversibly unfold and irreversibly inactivate enzymes including trypsin in water due to decomposition.
Since the rate of reaction was dependent on the degree of thermal denaturation, a natural substrate, N-α-benzoyl-L-arginine ethyl ester (BAEE), was used to study the activity of boiled tryptic solutions as a function of concentration in neutral media (pH 7.0). Different concentrations of trypsin (i.e. 2-40 mg/mL) were prepared in 0.5 mL of 50 mM Tris-HCl buffered Milli-Q water (pH 7.0). The solutions were boiled for 20 minutes before measuring the activity of the boiled trypsin by recording the change in absorbance at 253 nm due to the formation of N-α-benzoyl-L-arginine. The spectrophotometric activity data is illustrated in
As measured by the rate of hydrolysis of BAEE, the activity of the boiled tryptic solutions decreased at lower concentrations due to increased denaturation. Comparatively, the relative decrease in the rate of silanol condensation (
Time Study
The trypsin-catalyzed condensation of trimethylsilanol was studied over a 24-hour period at 25° C. Independent reactions were formulated with a 4:1 monomer to enzyme weight ratio (˜1000:1 silanol to trypsin mole ratio) in a neutral medium (pH 7.0) and performed for defined periods of time over 24 hours. The trypsin-catalyzed condensation of trimethylsilanol was nearly complete after three hours at 25° C. Based on the stoichiometry of the condensation reaction, two moles of trimethylsilanol were consumed for each mole of hexamethyldisiloxane (HMDS) produced (mass balance,
Based on the estimated solubility of trimethylsilanol in water (42.56 mg/mL), the concentration of trimethylsilanol (˜160 mg/mL) saturated the aqueous medium and created a two-phase reaction mixture. Since proteases will only interact with water-soluble substrates, the trypsin-catalyzed condensation of trimethylsilanol was postulated to occur in the aqueous phase. Although the condensation reaction was conducted in water, the enzyme-catalyzed reaction was promoted by the phase separation of the product. The immiscibility of the product, hexamethyldisiloxane, changed the equilibrium and promoted the condensation reaction in the presence of water. Since the aqueous medium was saturated with trimethylsilanol, the reactant would continue to enter the aqueous phase due to the dynamic equilibrium of the condensation reaction. In addition, the hydrolysis or reverse reaction would be severely hindered due to the immiscibility of the disiloxane product in the aqueous phase.
Rate of Condensation During Time Example
The rate of condensation during the time study was analyzed, in order to begin to study the kinetics of the reaction catalyzed by trypsin at 25° C. Based on the time-independent stoichiometry of the proposed reaction, the rate (V) of condensation (Equation 1) and theoretical rate (Equation 2) equations were defined.
V=−0.5*(δ[Me3SiOH]/δt)=1*(δ[HMDS]/δt) (Equation 1)
V=kR[Me3SiOH]α[trypsin]β (Equation 2)
In Equation 1, the rate of condensation was defined as the change in concentration (i.e. [Molarity]=moles/L) of the reactant or product with time (e.g. δ[HMDS]/δt). In Equation 2, the experimental rate constant (kR) and the partial orders of reaction (i.e. α and β) with respect to each reactant were defined. The overall order of reaction is the sum of the partial orders (i.e. α+β). Since trypsin was not consumed during the condensation reaction (i.e. a catalyst), the term was included in the rate constant (kR′) and the theoretical rate equation (Equation 2) was simplified as defined in Equation 3. The general rate equation is depicted as Equation 4.
V 32 kR′[Me3SiOH]α, kR′=kR[trypsin]β (Equation 3)
V=−0.5*(δ[Me3SiOH]/δt)=kR′[Me3SiOH]α, α=1 or 2 (Equation 4)
Since the solution is saturated with trimethylsilanol during most of the condensation reaction, the absolute amount of trimethylsilanol may be effectively constant. Given constant concentrations of trimethylsilanol and trypsin (i.e. catalyst), these reaction conditions would lead to a zero-order theoretical rate equation (Equation 2). In other words, the rate of condensation would be approximately constant. Since the initial (<3 hours) and final (>3 hours) rates of condensation or formation of HMDS are constant or linear, the data set appears to support this assessment. Based on the concentration of trypsin (i.e. [Et]=[trypsin]=1.7 mM) and the rate of condensation (i.e. V=−δ[Me3SiOH]/δt=0.0064 M/m˜2δ[HMDS]/δt =0.0072 M/m), a relative value the turnover number (kcat) or number of substrate molecules converted into product by an enzyme molecule in a unit time when the enzyme is fully saturated with substrate was calculated to be 4.0 reactions per minute (m−1) or 0.066 reactions per second (s−1) at 25° C.
Since trypsin may not be saturated due to the limited solubility of trimethylsilanol in water, the turnover number was treated as a relative value. Given a relative turnover number equal to 0.066 s−1, the time period between each condensation reaction catalyzed by trypsin was calculated to be 15 s. In comparison to the maximum turnover numbers of other enzymes with their physiological substrates, the turnover number of the trypsin-catalyzed condensation of trimethylsilanol at 25° C. was several orders of magnitude (i.e. ˜10-10,000,000) slower than the cited values. For example, the turnover number of the trypsin-catalyzed condensation of trimethylsilanol was approximately 1500 times slower than a natural chymotrypsin-catalyzed hydrolysis reaction.
Evaluation of the Monomer to Enzyme Mole Ratio
The rate of trimethylsilanol condensation was studied as a function of the monomer to enzyme mole ratio. The mole ratios were formulated with a constant amount of trimethylsilanol (160 mg/mL) and a variable amount of trypsin (2-198 mg/mL) in buffered water (pH 7.0). The closed (screw capped) reactions were conducted at 25° C. with magnetic stirring for three hours. The reaction products were isolated and quantitatively analyzed by GC (
Based on the chromatographic results, the rate of reaction was determined to be dependent on the amount of trypsin. In addition, the interaction between these experimental variables was complicated due to the potential for autolysis at dilute concentrations of trypsin (i.e. <20 mg/ml). Given the decreased stability of dilute trypsin solutions (
Since the reactions were conducted with a constant concentration of trimethylsilanol, a simplified theoretical rate equation (Equation 5) was used to perform a preliminary assessment of the partial-order of reaction with respect to trypsin. If the partial-order of reaction with respect to trypsin was first-order (β=1), the ratio of the rate of condensation (V) to the concentration of trypsin would be a constant value (Equation 6).
V=kR′[trypsin]β, kR′=kR[Me3SiOH]α (Equation 5)
kR′=V/[trypsin]1 (Equation 6)
Since the condensation reactions were conducted for a fixed period of time (i.e. three hours), the amount of hexamethyldisiloxane (μmole) formed will be related to the rate of condensation (i.e. V=[HMDS]3 hours). A review of the chromatographic results showed that the calculated hexamethyldisiloxane to trypsin mole ratios were indeed approximately constant. In other words, the rate of condensation seemed to be directly proportional to the concentration of trypsin (theoretical black line,
As observed, reducing the amount of trypsin by a factor of ten decreased the product yield by a factor of ten. This experimental observation supports the role of trypsin as a catalyst in the condensation of trimethylsilanol in a neutral medium and that it proceeds via a simple enzyme-substrate intermediate.
Kinetic Study of the Trypsin-Catalyzed Condensation of Non-Saturated Solutions of Trimethylsilanol
Since proteases only interact with water-soluble substrates, the concentration of trimethylsilanol was decreased (<42.56 mg/mL) to study the rate of condensation as a function of monomer concentration in a non-saturated medium. The amount of trimethylsilanol (˜10-40 mg/mL, ˜60-222 μmole) was adjusted to create homogeneous solutions in 40-mg/mL neutral (pH 7.0) solutions of trypsin. The reactions were conducted at 25° C. and 15° C. with magnetic stirring. After quenching the independent reactions, the reaction products were isolated and quantitatively analyzed by GC every 15 minutes over one-hour periods.
A differential method was used to determine the partial order of reaction with respect to trimethylsilanol. The logarithm of the theoretical rate equation (Equation 3) was calculated to yield a linear equation (Equation 7) in which the slope (a) was equal to the partial order of reaction with respect to trimethylsilanol.
Log(V)=α Log [Me3SiOH]+Log(kR′) (Equation 7)
After plotting Log(V) vs. Log [Me3SiOH] at 25° C. (α=0.9) and 15° C. (α=partial order of reaction with respect to trimethylsilanol was estimated to be first-order. The plots of the differential rate equations with respect to trimethylsilanol were linear. The correlation coefficient (R2) values were 94% and 98%, respectively. Since the rate of reaction was determined to be linearly dependent on the soluble amount of trimethylsilanol in the 40-mg/mL neutral (pH 7.0) solutions of trypsin, the reactions seem to be first-order with respect to trimethylsilanol. As hypothesized, the rate of condensation appears to be proportional to both the concentration of trimethylsilanol and trypsin.
Furthermore, the Lineweaver-Burk equation was used to study the ability of the trypsin-catalyzed condensation of trimethylsilanol to fit the Michaelis-Menten kinetic model. The chromatographic data sets acquired during the time studies at 25° C. and 15° C. yielded linear Lineweaver-Burk plots. Based on the linear equations of the experimental Lineweaver-Burk plots, the relative Michaelis constant (Km) and maximum rate (Vmax) values were calculated (Table 4).
Although the Michaelis-Menten kinetic values are relative, the large Km values indicate that the binding strength of the enzyme-substrate intermediate is weak. Comparatively, these Km values are several orders of magnitude (i.e. ˜1,000-25,000,000) larger than the Km values of other enzymes. For example, the relative Km value of the trypsin-catalyzed condensation of trimethylsilanol at 25° C. was approximately 1200 times larger than the chymotrypsin-catalyzed hydrolysis of acetyl-L-tryptophanamide.
Since the relative Km values are large and the Vmax values are slow, the formation of the trypsin-silanol intermediate appears to be the rate-limiting step in the condensation reaction. This is consistent with the fact that trypsin was not saturated with trimethylsilanol in aqueous media. Therefore, the rate of condensation or hydrolysis of the trypsin-silanol intermediate must be faster than the formation of the enzymatic intermediate.
Based on the stoichiometry of the silanol condensation reaction and the rate of condensation, the trypsin-catalyzed condensation of trimethylsilanol was hypothesized to have a reaction mechanism (
Trypsin pH Study
In nature, the optimum pH for trypsin-catalyzed hydrolysis is approximately 8. However, this is species dependent. As measured with a natural substrate (BAEE), the activities of different sources of trypsin were studied as a function of pH (
Based on the spectrophotometric data, the profiles of the mammalian tryptic activities including the recombinant enzyme as a function of pH were similar. The mammalian source of bovine trypsin was observed to have the highest activity at pH 7.0. In comparison, the optimum pH of Gadus morhua (i.e. Atlantic cod) trypsin appeared to be more acidic. Since calcium was required to achieve the maximum activity and stability of trypsin, the differences in the relative activities may be due to variable levels of calcium. Based on an inductively coupled plasma-atomic emission spectroscopic analysis, the amount of calcium present in the commercial sources of trypsin was shown to be very different.
Calcium activation of trypsin induces changes in the tertiary structure of the enzyme. Specifically, calcium creates a compact structure due to increased helical content or an altered β-structure. The conformational changes have been hypothesized to be responsible for the documented increased enzymatic activity and thermal stability. Since the activity of trypsin is optimal in the presence of >10 mM (ideally, 20 mM or 400 ppm) calcium, the decreased levels of calcium in porcine pancreas and recombinant bovine trypsin correlate with their activities in comparison with bovine pancreatic trypsin.
Subsequently, the ability of bovine pancreatic trypsin to catalyze the trimethylsilanol condensation reaction was studied as a function of pH. The reactions were formulated with a 4:1 monomer to enzyme weight ratio (˜1 000:1 silanol to trypsin mole ratio) in aqueous media buffered from pH 4.0 to pH 10.0 and conducted at 25° C. for three hours. The reaction products were isolated and quantitatively analyzed by GC (
Given the natural hydrolytic activity profiles measured with BAEE (
In comparison to the natural pH activity profile (bovine trypsin,
Trypsin-Catalyzed Hydrolysis and Condensation of Trimethylethoxysilane
The role of trypsin in the formation of molecules with a single siloxane bond was studied during the in vitro hydrolysis and condensation of a model alkoxysilane, trimethylethoxysilane.
Prior to reaction, the alkoxysilane was pre-treated with sodium hydrogencarbonate due to the potential presence of residual chloro-functional silanes. The reactions were formulated with a 4:1 trimethylethoxysilane to protein weight ratio (˜1000:l alkoxysilane to trypsin mole ratio) in neutral media (pH 7.0) and conducted at 25° C. for three hours. Based on the estimated solubility of trimethylethoxysilane in water (1 mg/mL), the concentration of trimethylethoxysilane (˜160 mg/mL) saturated the aqueous media and created two-phase reaction mixtures. The reaction products were isolated and quantitatively analyzed by GC (
Although various rates of hydrolysis were observed, substantial condensation of trimethylethoxysilane was not observed in the negative control, non-specific protein (i.e. BSA, γ-globulins), small molecule (i.e. CaCl2, imidazole, N-methylimidazole), and polypeptide (i.e. poly-L-lysine) reactions in comparison to the raw material. Although BSA and poly-L-lysine were not observed to catalyze the condensation of tetraethoxysilane or trimethylethoxysilane (
In the presence of trypsin, trimethylethoxysilane was hydrolysed (100%) and condensed (84%) during the formation of hexamethyldisiloxane in a neutral medium (pH 7.0) at 25° C. over three hours (
Based on the stoichiometry of the hydrolysis and condensation reactions, two moles of trimethylethoxysilane were consumed in the formation of two moles of trimethylsilanol, which produced one mole of hexamethyldisiloxane (mass balance,
The partial orders of the reactions with respect to reactants as well as the turnover numbers (kcat) in the hydrolysis and condensation reactions were calculated. Since trypsin may not be saturated due to the limited solubility of trimethylethoxysilane in water, the turnover number was treated as a relative value. Given a relative turnover number equal to 0.53 s−1, the time between each hydrolysis reaction catalyzed by trypsin was calculated to be approximately 2 s or 30 reactions per minute at 10° C. Although this is comparable with the maximum turnover number of lysozyme, the turnover number of the trypsin-catalyzed hydrolysis of trimethylethoxysilane at 10° C. was approximately 200 times slower than a chymotrypsin-catalyzed hydrolysis reaction. Given a relative turnover number equal to 0.048 s−1, the time period between a condensation reaction catalyzed by trypsin was calculated to be approximately 20 s or 3 reactions per minute at 10° C.
Based on the relative turnover numbers, the rate of the trypsin-catalyzed hydrolysis of trimethylethoxysilane (0.53 s−1) was one order of magnitude (ten times) faster than the condensation of trimethylsilanol (0.048 s−1) at 10° C. (
Alkoxysilane Study
Since trypsin catalyzed the formation of siloxane bonds, alternate mono-functional alkoxysilanes were chosen as substrates to investigate the ability of trypsin to selectively catalyze the in vitro hydrolysis and condensation of organo-functional alkoxysilanes under mild conditions. Initially, two additional trimethylalkoxysilanes were selected with comparatively polar (i.e. tetraethylene glycol monomethyl ether) and non-polar (i.e. hexanol) leaving groups to study the activity of trypsin as a function of the solubility of the reactant in a neutral medium (pH 7.0).
The two-phase reactions were formulated with a 4:1 monomer to enzyme weight ratio (>400:1 alkoxysilane to trypsin mole ratio) and conducted at 25° C. for three hours. The reaction products were isolated and quantitatively analyzed by GC (
Subsequently, four organo-functional alkoxysilanes were selected to study how the activity of trypsin varied as a result of different steric and electronic interactions with the substrate. The four organo-functional alkoxysilanes are phenyldimethylethoxysilane (PhMe2SiOEt), triphenylethoxysilane (Ph3SiOEt), 3-glycidoxypropyldimethylethoxysilane ((epoxy)Me2SiOEt), and aminopropyldimethylethoxysilane ((H2N(CH2)3)Me2SiOEt).
The two-phase reactions were formulated with a 4:1 monomer to enzyme weight ratio (>300:1 alkoxysilane to trypsin mole ratio) and conducted at 25° C. for three hours. The reaction products were isolated and quantitatively analyzed by GC (
Alternatively, two silicon-functional molecules with cyclic architectures were chosen to further investigate the ability of trypsin to catalyze the cleavage and formation of Si—O bonds. The two-phase reactions were formulated with a 4:1 monomer to enzyme weight ratio and conducted at 25° C. for three hours (Schemes 2-3). The reaction products were isolated and quantitatively analyzed by GC (
Based on the chromatographic results, trypsin catalyzed the ring-opening hydrolysis of 1,1-dimethyl-1-sila-2-oxacyclohexane and condensation of hydroxybutyldimethylsilanol during the formation of the carbinol-functional disiloxane (Scheme 2). Trypsin did not catalyze the condensation of heptamethylhydroxytetracyclosiloxane. Despite their cyclic architectures, these organosilicon molecules and the resultant intermediates and products were different. Comparatively, the cyclic siloxane is sterically larger than the cyclic alkoxysilane. Analogous to basic residues (Scheme 4), the carbinol-functional silanol intermediate was hypothesized to be an acceptable substrate due to its ability to hydrogen bond with the aspartic acid residue within the binding domain of the catalytic region of trypsin.
In comparison to a control reaction, trypsin reportedly did not catalyze the polycondensation of a silicic acid precursor, tetraethoxysilane, in an aqueous medium at pH 6.8. In this study, no reaction products were observed in a replicate reaction formulated with a 4:1 monomer to enzyme weight ratio and conducted at 25° C. for three hours. Specifically, trypsin did not hydrolyze or condense tetraethoxysilane during the three-hour reaction. Comparatively, following a seven-day reaction, trypsin was observed to catalyze the polycondensation of tetraethoxysilane during the formation of a solid composite containing silica and trypsin. The role of the active site of trypsin in the polycondensation of tetraethoxysilane was not definitive in this study. In review, trypsin was observed to selectively catalyze the hydrolysis and condensation of organo-functional alkoxysilanes under mild conditions.
Proteinaceous Inhibition Study
A proteinaceous inhibition study was conducted to investigate the role of the enzymatic active site in the hydrolysis and condensation of trimethylethoxysilane. Prior to reaction, trypsin was independently inhibited with an excess amount of the Bowman-Birk inhibitor (4:1 BBI to trypsin mole ratio) and the Popcorn inhibitor (2:1 PCI to trypsin mole ratio) in stirred neutral media (pH 7.0) for two hours. Based on standard enzymatic activity assays, trypsin was fully inhibited by the BBI (98%) and PCI (91%). The reactions were formulated with a 4:1 monomer to enzyme weight ratio (˜1000:1 trimethylethoxysilane to trypsin mole ratio) and conducted at 25° C. for three hours. The reaction products were isolated and quantitatively analyzed by GC (
Although the treated enzymes were observed to catalyze the hydrolysis of trimethylethoxysilane, the condensation of trimethylsilanol was completely inhibited in comparison to the control reactions. Notably, the percent hydrolysis decreased in the presence of the BBI- (24%) and PCI- (6%) inhibited trypsin. Following thermal denaturation, the activity of trypsin was comparable to the proteinaceous inhibition and previous denaturation experiments. Consequently, it appeared that non-specific interactions with trypsin, including the active site, promoted the hydrolysis of trimethylethoxysilane. However, the active site of trypsin was determined to selectively catalyze the in vitro condensation of trimethylsilanol under mild conditions.
Hydrolysis and Condensation of Trimethylethoxysilane with Different Sources of Trypsin
Although various sources (e.g. mammalian, fish) of trypsin are similar (e.g. tertiary structure), their selectivity and activity may be different. Consequently, the ability of porcine pancreas, Gadus morhua (i.e. Atlantic cod), and recombinant bovine trypsin to catalyse the hydrolysis of trimethylethoxysilane and condensation of trimethylsilanol was evaluated in neutral media (pH 7.0) at 25° C. Although the pH may not have been optimal for these different sources of trypsin, a neutral pH was used to minimise acid- and base-catalysed hydrolysis and condensation. The reaction products were isolated and quantitatively analysed by GC (
Based on the chromatographic results, trypsin from porcine pancreas (i.e. mammalian) as opposed to Atlantic cod (i.e. fish) was observed to catalyse the hydrolysis and condensation reactions. Similar to the pH profiles measured with a natural substrate (
Calcium activation of trypsin induces changes in the tertiary structure of the enzyme. Since the activity of trypsin is optimal in the presence of >10 mM calcium, the ability-of trypsin to catalyse the condensation of trimethylsilanol was studied in a neutral medium (pH 7.0) containing 20 mM CaCl2. The reactions were formulated with a 4:1 monomer to enzyme weight ratio (˜1000:1 silanol to trypsin mole ratio) and conducted at 25° C. for three hours. The reaction products were isolated and quantitatively analysed by GC (
In comparison to the original sources of trypsin, the activities as measured by the yield of the recombinant bovine and porcine pancreas trypsin-catalysed reactions increased 65% and 125%, respectively. Comparatively, the yield of the bovine pancreas and Atlantic cod trypsin-catalysed reactions did not change. In the absence of calcium chloride (
Enzyme-Catalyzed Polycondensation and Ring-Opening Polymerization of Silicon-Based Monomers
The ability of trypsin to catalyze the formation of siloxane bonds during the in vitro polycondensation and ring opening polymerization of silicon-based monomers was explored under mild conditions.
Trypsin-Catalyzed Polycondensation of Methyltriethoxysilane
Given the catalytic role of trypsin in the model siloxane condensation reactions, trypsin was used as a catalyst in the polycondensation of a trifunctional alkoxysilane, methyltriethoxysilane, under mild conditions. The reaction was formulated with a 4:1 methyltriethoxysilane (0.091 g, 511 μmol, 3.25 mmol Si) to trypsin (0.022 g, 0.9 μmol) weight ratio (˜550 monomer to enzyme mole ratio) in a neutral medium (pH 7.0) and conducted at 25° C. for seven days (Scheme 5).
The solid and liquid reaction products were isolated and selectively characterized by infrared spectroscopy, microscopy, and mass spectrometry techniques.
Based on the diffuse reflectance infrared Fourier transform spectra of trypsin and the solid reaction product, the isolated solid was determined to be a composite material containing a mixture of methylsilsesquioxane resin and trypsin. Comparatively, the spectral peaks of a methylsilsesquioxane resin were observed in the presence of a control spectrum acquired with trypsin. Specifically, the symmetric methyl deformation (MeSiO3/2, near 1270 cm−1), siloxane asymmetric stretch (SiOSi, near 1000-1130 cm−1), as well as the asymmetric methyl rock and silicon-carbon stretch (MeSiO3/2, near 778 cm−1 ) were observed in the presence of the spectral peaks associated with trypsin. The solid was observed to contain thick agglomerates ranging in size from approximately 200 μm to 1.5 mm. The rough surfaces of the agglomerate particles were composed of submicron round particles. Based on an energy dispersive spectroscopy (SEM-EDS) analysis, the surfaces of the particles were determined to contain silicon, oxygen, carbon, and sulfur. These elements are consistent with the functionality of methylsilsesquioxane and trypsin. The surface also contained sodium and chloride due to the use of a salt (NaCl) in the extraction procedure. Based on the silicon (Si) stoichiometry of the polycondensation reaction, the trypsin-catalyzed polycondensation reaction yielded 12% solid (0.011 g, 0.39 mmol Si). Comparatively, a solid precipitate was not observed in the negative control reaction.
The liquid reaction products were isolated and characterized by electrospray ionisation mass spectrometry (ESI MS). Although methyltriethoxysilane was not observed in the spectral results, substantial hydrolysis and condensation of methyltriethoxysilane was not observed in the absence of trypsin. Primarily, ethoxy-functional low molecular weight oligomers (e.g. dimers, trimers, tetramers) and cyclic siloxanes were observed in the ESI MS spectrum. In comparison to the negative control reaction, trypsin promoted the complete hydrolysis and, subsequent, polycondensation of methyltriethoxysilane. The distributions of linear, cyclic, and branched siloxane molecules were fully hydroxylated. Although trypsin promoted the hydrolysis of the alkoxy-functional silicones, the role of the active site of trypsin in the polycondensation of these molecules was not definitive in this study.
Although the ESI MS results are qualitative, the reaction products in the solid and liquid phases fully detail the polycondensation reactions. Prior to the precipitation of agglomerated silsesquioxanes in the presence of trypsin, the formation of linear, cyclic, and branched oligomers during the polycondensation of methyltriethoxysilane was analogous to the polymerization behavior of silica.
Trypsin-Catalyzed Ring-Opening Polymerization of Cyclic Siloxanes
The ability of trypsin to catalyze the cleavage and formation of Si—O bonds during the ring opening polymerization of cyclic siloxanes was explored under mild conditions. Specifically, five cyclic siloxanes: hexamethylcyclotrisiloxane, octamethylcyclotetrasiloxane, trimethyltri(trifluoropropyl)cyclotrisiloxane, tetramethyltetra(trifluoropropyl)cyclotetrasiloxane, and tetramethyltetraphenylcyclotetrasiloxane, were selected to study how the activity of trypsin varied as a result of different steric and electronic interactions with the substrate.
The two-phase reactions were formulated with a 4:1 monomer to enzyme weight ratio and conducted at 25° C. in neutral media (pH 7.0) for 8 days. The reaction products were isolated and qualitatively analyzed by GC (Table 5).
1The GC cyclic area percent values are qualitative.
Based on the chromatographic data, trypsin was not observed to catalyze the hydrolysis of the cyclic siloxane bonds. As previously reported, trypsin was unable to hydrolyze hexamethyldisiloxane in a neutral medium (pH 7.0) at 25° C. Since proteases will only interact with water-soluble substrates, the hydrolysis reactions would be severely hindered due to the immiscibility of the cyclic siloxanes in the aqueous phase. Although trypsin would theoretically catalyze of the hydrolysis of a siloxane bond due to the law of microscopic reversibility, the reverse reaction was not favored.