METHODS FOR GENETIC CONTROL OF PLANT PEST INFESTATION AND COMPOSITIONS THEREOF

FIELD OF THE INVENTION

The present invention relates generally to the field of molecular biology and more specifically to the genetic control of plant pests, and even more particularly to the genetic control of Heterodera nematode infestations in plants. More specifically, the present invention relates to methods for modifying expression of one or more polynucleotide and/or protein molecules in one or more cells or tissues of a pest species. The present invention discloses substantially the entire genome sequence of the plant nematode pest, Heterodera glycines, and describes the use of these sequences to modify the expression of one or more target polynucleotide or protein molecules in at least the cells of a Heterodera species by providing in its diet a dsRNA that comprises a part of, or all, or substantially all of one or more polynucleotide molecules of the present invention.

BACKGROUND OF THE INVENTION

Plants and animals are targets of many different pests, including but not limited to nematode and insect pest species. Crops are often the targets of nematode infestations. Chemical nematicides are not effective in eradicating the nematode infestations. Chemical pesticidal agents are not selective and exert their effects on non-target fauna as well, often effectively sterilizing for a period of time a field over which the chemical nematicidal agents have been applied. Some chemical pesticidal agents have been shown to accumulate in food, and to exhibit adverse effects on workers that manufacture and apply such chemical agents. Thus there has been a long felt need for methods for controlling or eradicating nematode pest infestation on or in plants, i.e., methods which are selective, environmentally inert, non-persistent, biodegradable, and that fit well into pest resistance management schemes. Plant biotechnology provides a means to control pest infestations by providing plants that express one or more pest control agents. Recombinant pest control agents have generally been reported to be proteins selectively toxic to a target pest that are expressed by the cells of a recombinant plant. Recently, small RNA molecules provided in the diet of the pest species Meloidogyne incognita have been shown to exhibit effects on the viability of the pest by affecting gene expression in the pest cells (Tobias et al. WO 01/37654 A2). Recombinant approaches to plant pest control can be selective, and are environmentally inert and non-persistent because they are fully biodegradable.

The phenomenon of dsRNA mediated gene silencing has been demonstrated in a number of plant and animal systems (Fire et al. 1998 Nature 391:806-811; Waterhouse et al. 1998 PNAS USA 95:13959-13964; Tabara et al. 1998 Science 282:430-431; Fire et al. WO 99/32619 A1; Trick et al. WO 2004/005485 A2). Methods for delivering dsRNA into the animal systems involved generating transgenic insects that express double stranded RNA molecules or injecting dsRNA solutions into the body of the animal or within the egg sac prior to or during embryonic development. Double stranded RNA mediated gene suppression has been demonstrated in plant parasitic nematodes either by providing dsRNA or miRNA's in the nematodes' diet or by soaking the nematodes in solutions containing such RNA molecules (Atkinson et al., (The University of Leeds) WO 03/052110 A2; Trick et al., (Kansas State University Research Foundation) US 2004-009876A1). Cyst nematodes (Heterodera and Globodera species) are particularly damaging pests of crop plants. Cyst nematodes include but are not limited to Heterodera avenae, H. cruciferae, H. glycines, H. hordecalis, H. latipons, H. oryzae, H. oryzicola, H. rostochinesis, H. zeae, H. schachtii, G. achilleae, G. artemisiae, G. mexicana, G. millefolii, G. pallida, G. rostochiensis, G. tabacum, G. tabacum solanacearum, G. tabacum tabacum, G. tabacum virginiae, Globodera sp. Bouro, Globodera sp. Canha, Globodera sp. Ladeiro, Globodera sp. New Zealand-EK-2004, and Globodera sp. Peru-EK-2004. These species are known to parasitize a wide variety of crops including, but not limited to barley, corn, oats, rice, rye, wheat, cabbage, cauliflower, soybean, sugar beet, spinach, mustards, and potato. Cyst nematodes are particularly problematic. Eggs persist and remain viable in the soil for many years. Genetic resistance by conventional crop breeding has limited success in identifying resistance genes effective against the wide variety of races and biotypes of the cyst nematodes. Of particular concern is the soybean cyst nematode, Heterodera glycines, herein referred to as SCN.

Therefore, there exists a need for improved methods and compositions useful to modulate gene expression by repressing, delaying or otherwise reducing gene expression within a particular plant nematode pest for the purpose of controlling the nematode infestation or to introduce novel agronomically valuable phenotypic traits.

SUMMARY OF THE INVENTION

The present invention comprises methods and compositions for inhibiting expression of one or more target genes and proteins at least in cyst nematodes such as members of the Heterodera and Globodera species. More specifically, the present invention comprises a method of modulating or inhibiting expression of one or more target genes in Heterodera glycines and Heterodera schactii, to cause cessation of feeding, growth, development, reproduction and infectivity and eventually result in the death of the nematode pest. The method comprises introduction of partial or fully, stabilized double-stranded RNA (dsRNA) or its modified forms, such as small interfering RNA (siRNA) or micro RNA (miRNA) sequences, into the cells of the nematode wherein expression of at least one or more target genes is inhibited in the target nematode pest, wherein inhibition of the one or more target genes exerts a deleterious effect upon the nematode pest, wherein the dsRNA, siRNA, or miRNA are derived from the target gene sequences, specifically inhibit such target genes in the target pest, and so are specific to nematode pests such as those of the Heterodera species. It is specifically contemplated that the methods and compositions of the present invention will be useful in limiting or eliminating Heterodera infestation in or on any cyst nematode host by providing one or more compositions comprising dsRNA molecules in the diet of the nematode, wherein the diet is all or part of a plant cell.

In another aspect of the present invention, DNA molecules of the present invention comprise molecules that function as promoter sequences, polypeptide coding sequences, non-coding regulatory sequences, or polyadenylation sequences isolated from the genome of the soybean cyst nematode, the polynucleotide sequence of which is at least from about 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, or about 100% identical to sequences selected from the group consisting of SEQ ID NO: 1 through SEQ ID NO:45568, the complement thereof, or a portion thereof. A DNA molecule selected from the group consisting of SEQ ID NO:97730 through SEQ ID NO:119145 exhibits promoter activity, and a DNA molecule selected from the group consisting of SEQ ID NO:45569 through SEQ ID NO:47643 comprises at least one protein coding sequence, whether or not a complete open reading frame is exhibited.

Accordingly, in another aspect of the present invention, a set of isolated and purified polynucleotide sequences as set forth in SEQ ID NO:45569 through SEQ ID NO:47643 are provided as target sequences for the design of DNA constructs that express a stabilized dsRNA, siRNA, or miRNA molecule for inhibition of expression of a target gene in a nematode pest. A stabilized dsRNA, siRNA, or miRNA molecule can comprise two or more polynucleotide molecules that are arranged in a sense and an antisense orientation relative to at least one promoter, wherein the polynucleotide molecule that comprises a sense strand and an antisense strand are linked or connected by a spacer sequence of at least from about five to about one thousand nucleotides, wherein the sense strand and the antisense strand are at least about the same length, and wherein each of the two polynucleotide sequences shares at least about 80% sequence identity, at least about 90%, at least about 95%, at least about 98%, or even about 100% sequence identity, to a polynucleotide sequence as set forth in one of SEQ ID NO:45569 through SEQ ID NO:47643.

The present invention provides a method for identifying a DNA molecule for use as a DNA construct expressing a dsRNA-mediated gene silencing sequence in a plant cell, comprising selecting a target polynucleotide molecule of a Heterodera glycines polynucleotide sequence comprising 21 or more contiguous nucleotides wherein said polynucleotide sequence is selected from the group consisting of SEQ ID NO:45569-50775, SEQ ID NO:45569-47643, and SEQ ID NO:47644-50775.

The present invention also provides a recombinant DNA molecule for use in plant transformation, constructed to contain at least one polynucleotide molecule transcribed as a single stranded RNA molecule. The single stranded RNA molecule is capable of forming in vivo a double stranded RNA molecule through intermolecular hybridization that, when provided in the diet of a nematode pest, inhibits the expression of at least one target gene in one or more cells of the target organism. The polynucleotide molecule is operably linked to at least one promoter sequence that functions in a transgenic plant cell to transcribe the polynucleotide molecule into one or more ribonucleic acid molecules. The RNA molecule(s) self assemble into double stranded RNA molecules and are provided in the diet of a target pest that feeds upon the transgenic plant. The provision of the dsRNA molecule in the diet of the pest achieves the desired inhibition of expression of one or more target genes within the pest organism, resulting in fecundicity, morbidity, and/or mortality of the target pest.

The present invention also provides a recombinant plant cell having in its genome at least one recombinant DNA sequence that is transcribed to produce at least one dsRNA molecule that functions when the cell and/or its contents are ingested by a target nematode or pest to inhibit the expression of at least one target gene in the target nematode or pest. The dsRNA molecule is transcribed from all or a portion of a polynucleotide molecule that at least in part exhibits from about 75 to about 100% identity to a target nematode specific polynucleotide sequence as set forth in SEQ ID NO:45569 through SEQ ID NO:50775.

The present invention also provides a recombinant DNA construct for expression of a dsRNA-mediated gene silencing sequence in a plant cell, which comprises at least two different target sequences that, when expressed in vivo as RNA sequences and provided in the diet of a target nematode pest, inhibit the expression of at least two different target genes in one or more cells or tissues of the target nematode pest. A first target sequence exhibits at least from about 75 to about 100 percent identity to a first specific polynucleotide sequence region as set forth in SEQ ID NO:45569-SEQ ID NO:50775, and a second target sequence that is different from the first target sequence exhibits at least about 75 to about 100 percent identity to a second specific polynucleotide sequence region as set forth in SEQ ID NO:45569 through SEQ ID NO:50775, wherein the two or more target sequences are assembled in a DNA construct and expressed together as a single RNA transcript, and are constructed to form one or more dsRNA's useful in suppression of the one or more target genes. The DNA construct is transformed into a soybean cell, and the cell is regenerated into a recombinant plant. The dsRNA molecules are thus provided in the diet of the target nematode pest in a target nematode pest inhibitory concentration. Ingestion by the target nematode pest of recombinant plant cells or tissues expressing the recombinant dsRNA achieves the desired inhibition of expression of one or more target genes in the nematode, resulting in the fecundicity, morbidity, and/or mortality of the target nematode pest.

Another aspect of the present invention is the use of the nucleotide sequences disclosed herein to identify target sequences that occur in the transcript RNA of other plant pests, in particular insect pests, fungal pests, and nematode pests, that could be targeted simultaneously and/or contemporaneously with a single expression construct designed to suppress related genes in multiple plant pests by identifying sequences of sufficient length and identity in two or more different plant pests, and ensuring that the DNA construct used to produce a recombinant plant expresses one or more dsRNA molecules that function to effectively suppress one or more related genes in the two or more different plant pests. In particular, some contiguous nucleotide sequences equal to or greater than about 21-24 nucleotides in length are identified herein to be present within the genome of the soybean cyst nematode (SCN) Heterodera glycines and have been identified to be present as well in Heterodera schactii, and to some extent, other pest species as well, such as in several other nematode pest species as well as in other plant pest species such as specific insect nucleotide sequences, and in animal pest species such as insect pest species. Such sequences may be useful for effectively suppressing a target sequence in these plant pests, particularly when expressed as a dsRNA molecule in a recombinant plant cell that is provided in the diet of the pest, and can provide resistance to the plant from pest infestation from all or substantially all pests in which such sequences appear, in particular if the sequences in common are within genes shown to be essential for survival, reproduction, mobility, and/or development and differentiation.

The target sequences disclosed in the present invention can be used to identify related target sequences that occur in the transcript RNA of other pest species, particularly nematode species including but not limited to pests such as Heterodera species such as H. avenae, H. ciceri, H. crucifera, H. cyperi, H. fici, H. goettingiana, H. hordecalis, H. humuli, H. latipons, H. litoralis, H. medicaginis, H. mediterranea, H. oryzae, H. oryzicola, H. riparia, H. rostochinesis, H. salixophila, H. schachtii, H. sorghi, H. trifolii, H. turcomanica, and H. zeae, Meloidogyne species such as M. arenaria, M. chitwoodi, M. artiellia, M. fallax, M. hapla, M. javanica, M. incognita, M. microtyla, M. partityla, M. panyuensis, and M. paranaensis, Globodera species such as G. pallida, G. rostochiensis, and G. tabacum, Pratylenchus species such as P. brachyrus, P. crenatus, P. coffeae, P. magnica, P. neglectu, P. penetrans, P. scribneri, P. thornei, and P. vulnus. Other plant pest nematode species that are within the scope of the present invention include but are not limited to Xiphinema species, Nacobbus species, Hoplolaimus species, Paratylenchus species, Rotylenchulus species, Criconemella species, Hemicycliophora species, Helicotylenchus species, Rotylenchus species, Belonolaimus species, Trichodorus species, Tylenchorhynchus species, Radopholus species, Longidorus species, Dolichodorus species, Aphenlenchoides species, Ditylenchus species, Anguina species, and Tylenchulus species. A DNA construct that expresses a dsRNA molecule in a plant cell that has a target sequence common to multiple plant pests provides plant resistance to pest infestation from each pest containing such target sequences. A particular target sequence can be amplified within a single dsRNA transcript, and can contain only a single contiguous sequence of at least from about 17 to about 21 to about 50 nucleotides in common between any combination of pests, or can be comprised of a chimera consisting of various contiguous sequences of at least from about 17 to about 21 to about 50 or more nucleotides, each such contiguous sequence either being in common between two or more pests, or unique to only a single pest, such that the chimera, when present as a dsRNA sequence and provided in the diet of any one or more of the targeted pests, results in the effective control such one or more pests.

The present invention also provides a method for producing a transgenic plant by introducing into the genome of the plants' cells a polynucleotide sequence consisting of all or a portion of at least one of the aforementioned SCN specific recombinant DNA sequences, linked to linked substantially the complement of that sequence. Transgenic plants are generated from the transformed plant cell, and progeny plants, seeds, and plant products, each comprising the polynucleotide sequence, are produced from the transgenic plants.

The methods and compositions of the present invention may be applied to any monocot and dicot plant, depending on the pest species to be controlled and the host range of the nematode pest. Specifically, the plants are intended to comprise without limitation alfalfa, aneth, apple, apricot, artichoke, arugula, asparagus, avocado, banana, barley, beans, beet, blackberry, blueberry, broccoli, brussel sprouts, cabbage, canola, cantaloupe, carrot, cassaya, cauliflower, celery, cherry, cilantro, citrus, clementine, coffee, corn, cotton, cucumber, Douglas fir, eggplant, endive, escarole, eucalyptus, fennel, figs, gourd, grape, grapefruit, honey dew, jicama, kiwifruit, lettuce, leeks, lemon, lime, Loblolly pine, mango, melon, mushroom, nut, oat, okra, onion, orange, an ornamental plant, papaya, parsley, pea, peach, peanut, pear, pepper, persimmon, pine, pineapple, plantain, plum, pomegranate, poplar, potato, pumpkin, quince, radiata pine, radicchio, radish, raspberry, rice, rye, sorghum, Southern pine, soybean, spinach, squash, strawberry, sugar beet, sugarcane, sunflower, sweet potato, sweetgum, tangerine, tea, tobacco, tomato, turf, a vine, watermelon, wheat, yams, and zucchini plants. Preferably, the present invention is related to a transgenic soybean plant that contains in its genome a DNA construct that expresses a dsRNA molecule from any sequence of the present invention.

The invention also provides a computer readable medium having recorded thereon one or more of the sequences as set forth in SEQ ID NO:1 through SEQ ID NO:171306 and, with reference to nucleotide sequences, the complements thereof, for use in a number of computer based applications, including but not limited to DNA identity and similarity searching, protein identity and similarity searching, transcription profiling characterizations, comparisons between genomes, and artificial hybridization analyses.

BRIEF DESCRIPTION OF THE SEQUENCES

SEQ ID NO:1-SEQ ID NO:45568 correspond to individual sequences (singletons) and assembled singletons forming contiguous overlapping sequences (contigs) derived from DNA sequence analysis of one or more libraries produced from the genome of the soybean cyst nematode strain OP25.

SEQ ID NO:45569-SEQ ID NO:97729 correspond to sequences predicted to encode various proteins, tRNA's, rRNA's and the like, which were identified using the bioinformatics described herein as applied to SEQ ID NO:1-SEQ ID NO:45568, and are further defined in blocks of sequences corresponding to coding sequences characterized as (a) essential to SCN survival (SEQ ID NO:45569-SEQ ID NO:50775) and (b) other coding sequences and elements (SEQ ID NO:50776-SEQ ID NO:97729); and where the essential sequences are further defined in blocks of sequences corresponding to unigenes, EST's, or cDNA's which were (c) linked through bioinformatics analyses described herein to counterpart sequences entirely or partially known in the art (SEQ ID NO:47644-SEQ ID NO:50775) and (d) unique sequences exhibiting no known relationship to sequences known in the art (SEQ ID NO:45569-47643).

SEQ ID NO:97730-SEQ ID NO:119145 correspond to sequences predicted to comprise all or substantially all of one or more SCN promoter sequences.

SEQ ID NO:119146-SEQ ID NO:124352 correspond to amino acid sequences predicted to be encoded from the (a) essential and (b) other coding sequences set forth in SEQ ID NO:45569-SEQ ID NO:97729, and are further defined in blocks of sequences corresponding to (c) peptides essential to SCN survival, as set forth in SEQ ID NO:121221-SEQ ID NO:124352, each based on one or more BLASTP relationship to one or more proteins known to be essential to survival of C. elegans or other organisms (translated from SEQ ID NO:47644-SEQ ID NO:50775), and (d) other peptides lacking any BLASTP relationship to proteins known in the art, as set forth in SEQ ID NO:119146-SEQ ID NO:121220 (translated from SEQ ID NO:45569-SEQ ID NO:47643).

DETAILED DESCRIPTION OF THE INVENTION

The following is a detailed description of the invention provided to aid those skilled in the art in practicing the present invention. Those of ordinary skill in the art may make modifications and variations in the embodiments described herein without departing from the spirit or scope of the present invention.

The inventors have discovered all or substantially all of the polynucleotide sequences that comprise the genomic DNA obtained from the soybean cyst nematode Heterodera glycines, aligned the sequences to derived large blocks of sequence corresponding to genomic contigs set forth herein, analyzed these contigs to identify and characterize untranslated regulatory sequences, for example, promoters, introns, transcriptional initiation sequences, and polyadenylation signals. Genomic polynucleic acid sequences encoding all or part of one or more proteins and characterized as being essential for survival, such as amino acid sequences involved in various metabolic or catabolic biochemical pathways, cell division, reproduction, energy metabolism, digestion, neurological function and the like, are identified in the genomic DNA sequences disclosed in the present invention, and regions of such sequences are demonstrated herein as being useful for selection for use in preparing DNA constructs for use in transforming cells, and that also express double stranded RNA molecules from such constructs in the transformed cells, and when provided in the diet of a target pest, whether artificial diet or a natural diet, especially a plant cell, plant tissues or other plant parts, such as leaves, roots, stems, flowers, fruits or seeds, results in the fecundicity, morbidity, and/or mortality of the pest.

As described herein, ingestion by a target nematode pest of compositions containing one or more dsRNA molecules, wherein at least one segment of the dsRNA molecule corresponds to a substantially identical segment of RNA produced in the cells of the nematode, will result in death, growth inhibition, stunting, inhibition of maturation or fecundity of the nematode. These results indicate that a polynucleotide molecule, either DNA or RNA, derived from SCN can be used to design a DNA construct according to the methods of the present invention to express a recombinant gene product in a transgenic host cell. The host cell can be transformed to contain one or more of the polynucleotide molecules derived from sequences disclosed herein. The DNA construct transformed into the host cell transcribes one or more RNA sequences that form into a dsRNA molecule in the cell or biological fluids within the transformed host, thus making the dsRNA available for ingestion by nematode when it feeds upon the transgenic host. Therefore, the transformed host cell now contains within its genome the genetic potential to defend the host cell and its parents, siblings and children from attack by the nematode.

The present invention relates to genetic control of nematode infestations in host organisms. More particularly, the present invention includes the DNA constructs, selection of target polynucleotides and methods for delivery of nematode control agents to a nematode. The present invention provides methods for employing stabilized dsRNA molecules in the diet of the nematode as a means for suppression of targeted genes in the nematode, thus achieving desired control of nematode infestations in, or about the host or symbiont targeted by the nematode. The preferred host is a plant wherein the plant is transformed with a recombinant DNA construct that expresses recombinant stabilized dsRNA, siRNA, and/or miRNA molecules. The recombinant DNA construct comprises a nucleotide sequence that is transcribed into RNA by the host cell. The term “recombinant DNA” or “recombinant nucleotide sequence” refers to DNA that contains a genetically engineered modification through manipulation as a result of methods for mutagenesis, use of restriction enzymes, and thermal amplification methods, and the like.

The dsRNA molecules, siRNA molecules, and/or miRNA molecules of the present invention are homologous or complementary to at least about a contiguous 17-21 nucleotide sequence selected from the group consisting of SEQ ID NO:45569 through SEQ ID NO:97729. Isolated and purified nucleotide sequences from a SCN are provided from a genomic library constructed from polynucleotide sequences of the pest. The ingestion of such nucleotide sequences results in the reduction or elimination of an essential gene product necessary for the nematode's growth and development or other biological function.

The present invention also contemplates a transformed plant cell and transformed plants and their progeny. The transformed plant cells and transformed plants express one or more of the dsRNA, siRNA, or miRNA sequences of the present invention from one or more of the DNA sequences as set forth in SEQ ID NO:1-SEQ ID NO:45568 and SEQ ID NO:45569 through SEQ ID NO:97729, or the complement thereof.

As used herein the words “gene suppression”, when taken together, are intended to refer to any method for reducing the levels of a gene product as a result of gene transcription to mRNA. Gene suppression is also intended to mean the reduction of protein expression from a gene or a coding sequence including posttranscriptional gene suppression and transcriptional suppression. Posttranscriptional gene suppression is intended to refer to that suppression mediated by the homology between all or a part of an RNA transcript transcribed from a gene or coding sequence targeted for suppression and the corresponding double stranded RNA used for suppression, and refers to the substantial and measurable reduction of the amount of available mRNA available in the cell for binding by ribosomes. The transcribed RNA can be in the sense orientation to effect what is referred to as co-suppression, in the anti-sense orientation to effect what is referred to as anti-sense suppression, or in both orientations producing a dsRNA to effect what is referred to as RNA interference (RNAi). Transcriptional suppression is intended to refer to that suppression mediated by the presence in the cell of a dsRNA, a gene suppression agent, exhibiting substantial sequence identity to a promoter DNA sequence or the complement thereof to effect what is referred to as promoter trans suppression. Gene suppression may be effective against a native plant gene associated with a trait, e.g., to provide plants with reduced levels of a protein encoded by the native gene or with enhanced or reduced levels of an affected metabolite. Gene suppression can also be effective against target genes in plant nematodes that may ingest or contact plant material containing gene suppression agents, specifically designed to inhibit or suppress the expression of one or more homologous or complementary sequences in the cells of a nematode or other pest.

Post-transcriptional gene suppression by anti-sense or sense oriented RNA to regulate gene expression in plant cells is disclosed in U.S. Pat. Nos. 5,107,065, 5,759,829, 5,283,184, and 5,231,020. The use of dsRNA to suppress genes in plants is disclosed in WO 99/53050, WO 99/49029, U.S. Patent Application Publication 2003/0175965 A1, and 2003/0061626 A1, U.S. patent application Ser. No. 10/465,800, and U.S. Pat. Nos. 6,506,559, and 6,326,193.

A preferred method of post transcriptional gene suppression in plants employs both sense-oriented and anti-sense-oriented, transcribed RNA, which is stabilized, e.g., as a hairpin and stem and loop structure. A preferred DNA construct for effecting post-transcriptional gene suppression is one in which a first segment transcribes an RNA molecule in an anti-sense orientation relative to the mRNA of the gene transcript targeted for suppression, the first segment further linked to a second segment spacer region that is not homologous or complementary to the first segment, and linked to a third segment that transcribes an RNA, wherein a portion is substantially complementarity to the first segment. Such a construct would be expected to form a stem and loop structure by hybridization of the first segment with the third segment and a loop structure forms comprising the second segment (WO94/01550, WO98/05770, US 2002/0048814A1, and US 2003/0018993A1).

As used herein, the term “nucleic acid”, “polynucleic acid”, or “polynucleotide” refers to a single or double-stranded polymer of deoxyribonucleotide or ribonucleotide bases (also referred to as nucleotides) read from the 5′ to the 3′ end. The polynucleic acid may optionally contain non-naturally occurring or altered nucleotide bases that permit correct read through by a polymerase. The term “nucleotide sequence” or “polynucleic acid sequence” may refer to both the sense and antisense strands of a polynucleic acid molecule as either individual single strands or in the duplex. The term “ribonucleic acid” (RNA) is inclusive of RNAi (inhibitory RNA), dsRNA (double stranded RNA), siRNA (small interfering RNA), mRNA (messenger RNA), miRNA (micro-RNA), tRNA (transfer RNA, whether charged or discharged with a corresponding acylated amino acid), and cRNA (complementary RNA) and the term “deoxyribonucleic acid” (DNA) is inclusive of cDNA and genomic DNA and DNA-RNA hybrids. The words “nucleic acid segment”, “nucleotide sequence segment”, or more generally “segment” will be understood by those in the art as a functional term that includes genomic sequences, ribosomal RNA sequences, transfer RNA sequences, messenger RNA sequences, operon sequences and smaller engineered nucleotide sequences or portions thereof that control or affect the expression of a gene product or that may be adapted to express proteins, polypeptides or peptides. A polynucleic acid may optionally contain naturally occurring or altered nucleotide bases that prevent polymerization by a first polymerase copying the strand that contains such base(s), i.e., one or more bases that cannot be templated by the first polymerase while polymerizing the nascent or growing strand, so that any nucleotide sequence extending beyond the non-templated base(s) results in a cohesive end that can be used to link the polynucleic acid to one or more other nucleic acid sequences linked to the complement of the cohesive end, resulting in a chimeric nucleotide sequence. The naturally occurring or altered nucleotide base(s) can then be templated to link the fragments comprising the chimeric nucleotide sequence by exposing the chimera to a second polymerase that recognizes the naturally occurring or altered nucleotide base(s) and copies that/those base(s) with fidelity (Jarrell et al. U.S. Pat. No. 6,358,712; Newton et al. 1993 21:1155-1162). This method may be particularly useful when assembling multi-component sequences for expression of an RNA sequence that folds into a dsRNA sequence and functions to suppress one or more genes in one or more target organisms.

As used herein, the term “nematode” refers to plant parasitic nematodes, in particular to members of the Tylenchoidea superfamily, and more specifically to the Heteroderidae family of nematodes that include the cyst nematodes (including at least Heterodera and Globodera species) and the rootknot nematodes (Meloidogyne species). More specifically to Heterodera species and even more specifically to Heterodera glycines, the soybean cyst nematode. Nematode species that were shown to have homologous target sequences with H. glycines polynucleotides of the present invention were: rootknot nematode species—Meloidogyne species such as M. arenaria, M. chitwoodi, M. artiellia, M. fallax, M. hapla, M. javanica, M. incognita, M. microtyla, M. partityla, M. panyuensis, and M. paranaensis; cyst nematode species—Heterodera species such as H. schachtii, Globodera species such as G. rostochiensis, G. pallida, and G. tabacum, Heterodera species such as H. trifolii, H. medicaginis, H. ciceri, H. mediterranea, H. cyperi, H. salixophila, H. zeae, H. goettingiana, H. riparia, H. humuli, H. latipons, H. sorghi, H. fici, H. litoralis, and H. turcomanica; lesion nematode species—Pratylenchus species such as P. scribneri, P. magnica, P. thornei, P. crenatus, P. brachyrus, P. vulnus, P. penetrans, P. coffeae, and P. neglectus; other plant parasitic nematode species include: Hirschmanniella species, Radopholus species such as R. similis, and Pratylenchoid magnicauda. Animal intestinal parasitic nematode species for which polynucleotides have been identified as a result of comparisons to the sequence data disclosed herein include Ascaris lumbricoides, and Ascaris suum.

As used herein, a “pest resistance” trait is a characteristic of a transgenic plant, transgenic animal, transgenic host or transgenic symbiont that causes the plant, animal, host, or symbiont to be resistant to attack from a pest that typically is capable of inflicting damage or loss to the plant, animal, host or symbiont. Such pest resistance can arise from a natural genetic variation or more typically from incorporation of recombinant DNA that confers pest resistance. Fire et al. (U.S. Pat. No. 6,506,599) generically described inhibition of pest infestation, and demonstrated gene suppression in the non-pest nematode species Caenorhabditis elegans. Similarly, Plaetinck et al. (US 2003/0061626A1) suggests using dsRNA to inhibit gene function in a variety of nematode pests. Mesa et al. (US 2003/0150017 A1) describe using DNA sequences to transform host cells to express dsRNA sequences that are substantially identical to target sequences in specific pathogens, and particularly describe constructing recombinant plants expressing such dsRNA sequences for ingestion by various plant pests, facilitating down-regulation of a gene in the genome of the pest, and improving the resistance of the plant to the pest infestation. As used herein, the term “expression” refers to the transcription and stable accumulation of a nucleotide sequence comprising both sense and antisense RNA derived from the nucleic acid sequences disclosed in the present invention, whether or not the RNA sequence is capped, spliced, and polyadenylated and trafficked into the cytoplasm of the cell. Expression may also refer to translation of mRNA into a polypeptide or protein. As used herein, the term “sense” RNA refers to an RNA transcript corresponding to a sequence or segment that, when produced by the target nematode, is in the form of a mRNA that is capable of being translated into polypeptide by the target nematode cell. As used herein, the term “antisense RNA” refers to an RNA transcript that is complementary to all or a part of a mRNA that is normally produced in a cell of a nematode. The complementarity of an antisense RNA may be with any part of the specific gene transcript, i.e., at the 5′ non-coding sequence, 3′ non-translated sequence, introns, or the coding sequence. As used herein, the term “RNA transcript” refers to the product resulting from RNA polymerase-catalyzed transcription of a DNA sequence. When the RNA transcript is a perfect complementary copy of the DNA sequence, it is referred to as the primary transcript or it may be an RNA sequence derived from post-transcriptional processing of the primary transcript and is referred to as the mature RNA.

Exposure of a plant cyst forming nematode to the dsRNA, siRNA, or miRNA sequences of the present invention may occur during the nematodes' juvenile J2, J3, J4, adult female or adult male developmental stages. Exposure may occur as the J2 or male nematode is migrating through the plant vasculature, for example the cortical cells, or during or after establishment of a feeding site within syncytial cells. Exposure may occur by the production of the dsRNA in neighboring transfer-like cells with movement into the feeding site. dsRNA, siRNA, or miRNA may enter the nematode through a variety of means including, for example, through the stylet and pharnyx, the anus, the extratory duct, or amphidial and phasmid channels. dsRNA produced in the tissues of the feeding site may enter the nematode by transport through the feeding tube (Hussey, R S and Grundler et. al., 1998, Nematode parasitism of plants, Ch. 9, The Physiology and Biochemistry of Free-living and Plant-parasitic Nematodes, eds R N Perry and D J Wright), directly from the cytoplasm, from extracellular regions, or from other plant compartments. Movement of dsRNA, siRNA, or miRNA into the nematode may require that the RNA exhibit a molecular weight of less than or substantially less than 25 Kda (feeding tube size threshold). Creating an siRNA or miRNA in the plant that is bioavailable to the nematode may require preventing the siRNA from entering or remaining within the plant RISC complex, a protein complex well in excess of 25 KD. For example, this may be accomplished through a number of means such as (1) by co-expressing a small RNA-binding protein that exhibits a greater affinity for the plant RISC complex compared to the nematode specific siRNA, (2) by producing in the transgenic cell a nematode specific siRNA that is incompatible with the plant RISC complex yet functional in the nematode RISC complex, or (3) by down-regulating RISC complex expression in the feeding site established by the nematode. Small RNA-binding proteins may be optimized for binding to a specific siRNA or miRNA by modifying amino acid residues by phage display or other peptide selection methods.

As used herein, the phrase “inhibition of gene expression” or “inhibiting expression of a target gene in the cell of a nematode” may refer to the absence (or observable decrease) in the level of protein and/or mRNA product from the target gene. In the event that a particular transcript or translation product is not detectable, whether or not the lack of detection is a result of the expression of a dsRNA specifically designed to suppress the levels of such transcript or translation product, the phrase “inhibition of gene expression” or “inhibiting expression of a target gene in the cell of a nematode” may refer to the observation of a phenotypic effect or the lack thereof within the plant or within or about the target pest that feeds upon the transgenic plant. Specificity refers to the ability to inhibit the target gene without manifest effects on other genes of the cell and without any effects on any gene within the cell that is producing the dsRNA molecule. The inhibition of gene expression of one or more target genes in the nematode may result in novel phenotypic traits in the nematode.

Without limiting the scope of the present invention, there is provided, in one aspect, a method for controlling plant infestation by a nematode or other plant pest using stabilized dsRNA strategies. The method involves generating stabilized dsRNA molecules as one type of nematode control agents, that when provided in the diet of the nematode, induce gene silencing. As used herein, the phrase “generating a stabilized dsRNA molecule” refers to the methods of employing recombinant DNA technologies to construct a DNA nucleotide sequence that transcribes a stabilized dsRNA. As used herein, the term “silencing” refers the effective “down-regulation” of expression of one or more targeted nucleotide sequences within one or more cells of a nematode or other plant pest and, hence, the elimination of the ability of the targeted nucleotide sequence(s) to cause its normal effect within the cell.

The present invention also provides in part a delivery system for providing a nematode control agent to a nematode through exposure of the nematode to a host, such as a plant containing the one or more control agents of the present invention by ingestion of the plants' cells or the contents of those cells. One embodiment of the present invention provides for generating a transgenic plant cell or a plant that contains a recombinant DNA construct transcribing the stabilized dsRNA molecules of the present invention. As used herein, the phrase “generating a transgenic plant cell or a plant” refers to the methods of employing recombinant DNA technologies to construct a plant transformation vector transcribing the stabilized dsRNA molecules of the present invention, to transform a plant cell or a plant with such vector, and to generate the transformed plant cell or transgenic plant containing a part of the vector that transcribes the stabilized dsRNA molecules. In particular, the method of the present invention may comprise a recombinant DNA construct in a cell of a plant that results in dsRNA transcripts that are substantially homologous to an RNA sequence expressed by a nucleotide sequence contained within the genome of a nematode. Where the nucleotide sequence within the genome of a nematode comprises a gene essential to the viability and infectivity of the nematode, its down-regulation results in a reduced capability of the nematode to survive and/or infect and/or cause damage to host cells. Hence, such down-regulation results in a “deleterious effect” on the maintenance, viability, and infectivity of the nematode, in that it prevents or reduces the nematode's ability to feed off of and survive on nutrients derived from the host cells. By virtue of this reduction in the nematode's viability and infectivity, resistance and/or enhanced tolerance to infection by a nematode or other plant pest is facilitated in the cells of a plant.

It is envisioned that the compositions of the present invention can be incorporated within the seeds of a plant species either as a product of expression from a recombinant gene incorporated into the genome of the plant cells, or incorporated into a coating or seed treatment that is applied to the seed before planting. A plant derived from a single plant cell transformed to contain a recombinant or heterologous gene is considered herein to be a transgenic event.

The present invention also includes seeds and plants having more that one agronomically important trait. Such combinations are referred to as “stacked” traits. These stacked traits can include a combination of traits that are directed at the same target nematode pest, or they can be directed at different target nematode pests, or to one or more insect pests, or can provide herbicide tolerance to the plant, for example tolerance to glyphosate herbicide. The stacked traits can be achieved by breeding to plants that have the trait or by building a chimeric DNA construct that contains multiple plant expression cassettes and transforming the expression cassettes into the genome of the plant.

Cells of a plant seed of the present invention may express one or more dsRNA's, the sequence of any one of which is derived from a target sequence, i.e., a nematode specific sequence disclosed herein in SEQ ID NO:1-SEQ ID NO:45569, and also may express a nucleotide sequence that provides herbicide tolerance, for example, resistance to glyphosate, N-(phosphonomethyl) glycine, including the isopropylamine salt form of such herbicide. Herbicides for which transgenic plant tolerance has been demonstrated include but are not limited to: glyphosate, glufosinate, sulfonylureas, imidazolinones, bromoxynil, delapon, cyclohezanedione, protoporphyrionogen oxidase inhibitors, and isoxasflutole herbicides. Polynucleotide molecules encoding proteins involved in herbicide tolerance are known in the art, and include, but are not limited to a polynucleotide molecule encoding 5-enolpyruvylshikimate-3-phosphate synthase, bromoxynil nitrilase, phytoene desaturase, norflurazon, acetohydroxyacid synthase and the bar gene for tolerance to glufosinate and bialaphos (U.S. Pat. Nos. 5,627,061, 5,633,435, 6,040,497, 5,094,945, and 4,810,648).

As used herein, the term “pest control agent”, or “gene suppression agent” refers to one or more particular RNA molecules consisting of a first RNA segment and a second RNA segment that are complimentary to each other and are linked by a third RNA segment. The complementarity between the first and the second RNA segments results in the ability of the two segments to hybridize in vivo and in vitro to form a double stranded molecule, i.e., a stem comprising the first and the second segments linked together by the third segment which forms a loop between the first and second segments, so that the entire structure forms into a stem and loop structure. Structures consisting of a first and a second segment that hybridize more tightly to each other may form into a stem-loop knotted structure. The first and the second segments, when hybridized together, correspond invariably, and not respectively, to a sense and an antisense sequence with respect to the target RNA transcribed from the target gene in the target nematode that is suppressed by the ingestion of the dsRNA molecule, or ingestion of an siRNA molecule derived from the dsRNA molecule. The pest control agent can also be a substantially purified (or isolated) nucleic acid molecule and more specifically nucleic acid molecules or nucleic acid fragment molecules thereof from a genomic DNA (gDNA) or cDNA library. Such substantially purified molecules can be applied to a seed, whether a seed from a transgenic plant or otherwise, in the form of a seed treatment, together with a pharmaceutically acceptable carrier for stabilizing the dsRNA molecules, resulting in the dsRNA being bioavailable within a plant grown from the seed, or bioavailability within the rhizosphere of the root system of the plant grown from the seed. A seed may be treated with one or more agents, each exhibiting different activities designed to provide the seed, the germinating seedling, and the growing plant or root with one or more advantages in comparison to other plants, such as pest resistance, including bacterial, fungal, and nematode resistance, fertilizers, growth stimulants, gene stimulants or suppressors, herbicide functions to which the seed, germ, and or roots and seedling are resistant, and the like. Alternatively, the fragments may comprise smaller dsRNA oligonucleotides comprising from about 15 to about 750 or more consecutive nucleotides selected from the group consisting of SEQ ID NO:1-SEQ ID NO:45569 and the complements thereof, or from about 15 to about 30 nucleotides, or from about 21 to about 24 consecutive nucleotides. The pest control agent may also refer to a DNA construct that comprises the polynucleic acid molecules or nucleic acid fragment molecules of the present invention and the DNA construct is a transgene incorporated into the genome of a host cell. The pest control agent may further refer to a plant comprising such a DNA construct in its genome or in the genome of a subcellular organelle that comprises the polynucleic acid molecules or nucleic acid fragment molecules described in the present invention. The method of the present invention provides for the production of a dsRNA transcript, the nucleotide sequence of which is substantially homologous to a targeted RNA sequence encoded by a target nucleotide sequence within the genome of a target pest.

As used herein, the term “genome” as it applies to cells of a nematode, a plant pest, or a host encompasses not only chromosomal DNA found within the nucleus, but organelle DNA found within subcellular components of the cell. The sequences of the present invention, when introduced into plant cells, can therefore be either chromosomally integrated or organelle-localized. The term “genome” as it applies to bacteria encompasses both the chromosome and plasmids within a bacterial host cell. The DNA's of the present invention introduced into bacterial host cells can therefore be either chromosomally integrated, localized to a plasmid, or to a viral vector capable of replication in the bacterial host.

In certain preferred embodiments expression of the gene targeted for suppression in the plant pest is inhibited by at least about 10%, at least about 33%, at least about 50%, at least about 80%, at least about 90%, at least about 95%, or by at least about 99% or more within cells of the nematode so a significant inhibition takes place. Significant inhibition is intended to refer to inhibition sufficient to result in a detectable phenotype (e.g., cessation of growth, paralysis, sterility, behavioral effects, second generation effects, effects observed on nematodes ingesting dsRNA or on their progeny, morbidity, or mortality, etc.) or a detectable decrease in RNA and/or protein corresponding to the target gene being inhibited. Although in certain embodiments of the invention inhibition occurs in substantially all cells of the nematode, in other preferred embodiments inhibition occurs in only a subset of cells that are contacted with the dsRNA, or that are expressing the target gene transcript.

The advantages of the present invention may include, but are not limited to the ease of introducing dsRNA into the nematode or other pest cells, the low concentration of dsRNA, siRNA, or miRNA which can be used, the stability of dsRNA, siRNA, or miRNA and the effectiveness of the inhibition. The present invention provides a method for selecting polynucleotide sequences of a target gene sequence and is not limited to in vitro use of specific sequence compositions identified by the method or to the set of exemplary target genes of the present invention. Segments of the nucleotide sequences of the present invention may be selected for their level of gene inhibition/suppression by scanning segments of the H. glycines sequences to identify segments that exhibit preferred levels of gene suppression or pest inhibition when provided as a dsRNA molecule in the diet of one or more target pests such as H. glycines.

As used herein, the term “sequence identity”, “sequence similarity” or “homology” is used to describe sequence relationships between two or more nucleotide or amino acid sequences. The percentage of “sequence identity” between two sequences is determined by comparing two optimally aligned sequences over a comparison window, wherein the portion of the sequence in the comparison window may comprise additions or deletions (i.e., gaps) as compared to the reference sequence (which does not comprise additions or deletions) for optimal alignment of the two sequences. The percentage identity of a reference sequence to another is calculated by determining the number of positions at which the reference sequence (whether nucleic acid or amino acid sequence) is identical to another sequence to yield the number of matched positions, dividing the number of matched positions by the total number of positions in the window of comparison, and multiplying the result by 100 to yield the percentage of sequence identity. A sequence that is identical at every position in comparison to a reference sequence is said to be, with respect to a nucleotide sequence or amino acid sequence, identical to the reference sequence and vice-versa. A first nucleotide sequence when observed in the 5′ to 3′ direction is said to be the “complement” of, or complementary to, a second or reference nucleotide sequence observed in the 3′ to 5′ direction if the reverse complement of the first nucleotide sequence is identical at every nucleotide position with the second or reference sequence. As used herein, two nucleic acid sequence molecules are said to exhibit “complete complementarity” when every nucleotide of one of the sequences, when read 5′ to 3′, is complementary to every nucleotide of the other sequence when read 3′ to 5′. A nucleotide sequence that is complementary to a reference nucleotide sequence will exhibit a sequence identical to the reverse complement sequence of the reference nucleotide sequence.

In practicing the present invention, a target gene may be derived from a nematode or other pest species that causes damage to one or more different crop plants and/or yield losses to such plants. Several criteria may be employed in the selection of target genes. The gene may be one whose protein product has a rapid turnover rate, so that dsRNA inhibition will result in a rapid decrease in protein levels. In certain embodiments it is advantageous to select a gene for which a small decrease in expression level results in deleterious effects for the pest. It may be desirable to target a broad range of nematode species and so a nucleotide sequence is selected that is highly conserved across the targeted range of species. Conversely, for the purpose of conferring specificity, in certain embodiments a nucleotide sequence is selected that contains regions that are poorly conserved between individual targeted pest species, or between the targeted pest and other organisms. In certain embodiments it may be desirable to select a nucleotide sequence that exhibits no known homology to sequences in other organisms. As used herein, the term “derived from” refers to a specified nucleotide sequence that may be obtained from a particular source or species.

Target genes for use in the present invention may include, for example, those that play important roles in the viability, growth, development, reproduction and infectivity of a particular pest. These target genes may be one or more of any house keeping gene, transcription factor and pest specific gene that provides an observable phenotype, in particular a phenotype that results in the suppression of feeding on or the inability to utilize a transgenic soybean plant expressing a SCN derived dsRNA as a nutrient source. For example, target genes that are anticipated herein to be effective in producing such phenotypes are similar to those that have been shown to affect the viability, growth, development, mobility, neurological stimulation, muscular function, and reproduction in C. elegans, including but not limited to the following phenotypes: (Adl) adult lethal, (Age), (Bli) blistered, (Bmd) body morphology defect, (Ced) Cell death abnormality, (Clr) clear, (Daf) DAuer Formation, (Dpy) dumpy, (Egl) egg laying defect, (Emb) embryonic lethal, (Evl) everted vulva, (Fem) feminization of XX and XO animals, (Fgc) Fewer Germ Cells, (Fog) feminization of germline, (Gon) GONad development abnormal, (Gro) slow growth, (Him) high incidence of male progeny, (Hya) HYperActive, (Let) larval lethal, (Lin) lineage abnormal, (Lon) long body, (Lpd), (Lva) larval arrest, (Lvl) larval lethal, (Mab) Male ABnormal, (Mei) Defective meiosis, (Mig) MIGration of cells abnormal, (Mlt) molt defect, (Morphology), (Mut) Mutator, (Muv) MUltiVulva, (Oma) Oocyte MAturation defective, (Pat) Paralyzed, Arrested elongation at Two-fold, (Pch) PatCHy coloration, (Pnm) Pronuclear migration alteration in early embryo, (Prl) paralyzed, (Prz) PaRaLyzed, (Pvl) protruding vulva, (Pvu) protruding vulva, (Rde), (Reproductive), (Rol) roller, (Rot) centrosome pair and associated pronuclear rotation abnormal, (Rup) exploded, (Sck) sick, (Sle) Slow embryonic development, (Slu) SLUggish, (Sma) small, (Spd) SpinDle, abnormal embryonic, (Spo) Abnormal embryonic spindle position and orientation, (Step) sterile, (Stp) sterile progeny, (Unc) uncoordinated, (Unclassified), (Vul) vulvaless, (WT), (defect) morphological or behavioral defects. SCN genome sequences predicted to encode various gene products set forth herein annotated to the C. elegans specific genes previously shown to exert a negative effect or observable phenotype in Drosophila or in C. elegans are anticipated to be effective targets for achieving a similar phenotype when expressed in planta as a dsRNA for the purpose of suppressing a gene in SCN specifically targeted by the dsRNA. Gene sequences unique to SCN and not annotated to sequences or gene products from other organisms are also anticipated to be effective for achieving control of SCN when such sequences are provided in the diet of the SCN as a dsRNA because the target genes are unique to SCN pest metabolism, physiology, and pathogenicity.

DNA segments of the present invention are desired for use in constructing dsRNA expression sequences, particularly if the DNA segments exhibit at least from about 70% identity, or at least from about 75% identity, or at least from about 80% identity, or at least from about 90% identity, or at least from about 95% identity, or at least from about 98% identity, or at least about 100% identity to contiguous 17-24 nucleotide sequences found within the nematode genome or other pest sequences targeted for suppression. Sequences less than about 80% identical to a target gene are anticipated to be less effective and so less desirable. Inhibition is specific to the nematodes' gene or gene families, the sequence of which corresponds substantially to the dsRNA. Expression of unrelated genes is not affected. This specificity allows the selective targeting of a nematode or other pest species, resulting in the absence of an effect on non-target organisms exposed to the compositions of the present invention.

The regions predicted to be more effective at dsRNA-mediated gene silencing include regions that exhibit higher siRNA efficiency. Higher siRNA efficiency may be achieved by any technique, including, but not limited to, computational methods such as algorithms designed to predict siRNA efficiency based on thermodynamic characteristics of a given dsRNA (or DNA) sequence, generally considering sequences of from about 17, to about 18, to about 19, to about 20, to about 21, to about 22, to about 23, or even to about 24 contiguous nucleotides corresponding to a sequence that is being targeted for suppression (Schwarz et al., 200, Cell 115:199-208; Chalk et al. 2004, Biochem. Biophys. Res. Comm. 319:264-274; Ui-Tei et al., NAR 2004, 32:936-948; Reynolds et al., 2004, Nature Biotechnology 22:326-330).

Inhibition of a target gene using the stabilized dsRNA technology of the present invention is sequence-specific in that nucleotide sequences corresponding to the duplex region of the RNA are targeted for genetic inhibition. RNA containing a nucleotide sequences identical to a portion of the target gene is preferred for inhibition. RNA sequences with insertions, deletions, and single point mutations relative to the target sequence are also effective for gene specific inhibition. In performance of the present invention, it is preferred that the inhibitory dsRNA and the portion of the target gene share at least from about 75% sequence identity, or from about 80% sequence identity, or from about 90% sequence identity, or from about 95% sequence identity, or from about 99% sequence identity, or even about 100% sequence identity. Alternatively, the duplex region of the RNA may be defined functionally as a nucleotide sequence that hybridizes with a portion of the target gene transcript. A greater sequence homology across a target gene sequence that is less than full-length in comparison to the target gene compensates for a less homologous sequence that more closely approximates the full length of the target gene. The length of a nucleotide sequence that is identical to a portion of the target gene sequence can be from about 21, to about 25, to about 50, to about 100, to about 200, to about 300, or more contiguous bases. Normally, a sequence of greater than 20-100 nucleotides is preferable, although a sequence of greater than about 200-300 nucleotides may be preferred, depending on the length of the target gene. The invention has the advantage of being able to tolerate sequence variations due to genetic mutation, strain polymorphism, or evolutionary divergence. Therefore the nucleic acid molecule introduced into a plant for expression as a pest specific dsRNA gene suppression construct may not need to exhibit absolute homology, and may not need to represent the full length of the sequence targeted for suppression.

The dsRNA molecules may be synthesized either in vivo or in vitro. The dsRNA may be formed by a single self-complementary RNA strand or two complementary RNA strands expressed from separate expression constructs. Endogenous RNA polymerase of the cell may mediate transcription in vivo, or cloned RNA polymerase can be used for transcription in vivo or in vitro. Inhibition may be achieved by specific transcription in an organ, tissue, or cell type; stimulation of an environmental condition (e.g., infection, stress, temperature, chemical inducers); and/or engineering transcription at a developmental stage or age of the transgenic plant expressing the dsRNA construct. The RNA sequences expressed from the recombinant construct may or may not be polyadenylated. The RNA sequences expressed from the recombinant construct may or may not be capable of being translated into a polypeptide by a cell's translational apparatus.

The RNA, dsRNA, siRNA, or miRNA of the present invention intended for use in controlling plant pest infestation may be produced chemically or enzymatically through manual or automated reactions or in vivo in an organism other than the plant for which pest control is intended. RNA may also be produced by partial or total organic synthesis. Any modified ribonucleotide can be introduced by in vitro enzymatic or organic synthesis. The RNA may be synthesized by a cellular RNA polymerase or a bacteriophage RNA polymerase (e.g., T3, T7, SP6). If synthesized chemically or by in vitro enzymatic synthesis, the RNA may be purified prior to introduction into the cell or formulated in an agronomically acceptable carrier and applied to the soil, to the roots, or to the seed prior to planting. For example, RNA can be purified from a mixture by extraction with a solvent or resin, precipitation, electrophoresis, chromatography, or a combination thereof. Alternatively, the RNA may be used with no, or a minimum of, purification to avoid losses due to sample processing. The RNA may be dried for storage or dissolved in an aqueous solution. The solution may contain buffers or salts to promote annealing, and/or stabilization of the duplex strands.

For transcription from a transgene in vivo or from an expression cassette, a regulatory region (e.g., promoter, enhancer, silencer, leader, intron and polyadenylation) may be used to modulate the transcription of the RNA strand (or strands). Therefore, in one embodiment, the polynucleotide sequences constructed to facilitate transcription of the RNA molecules of the present invention are operably linked to one or more promoter sequences functional in a plant host. The polynucleotide sequences may be placed under the control of an endogenous promoter normally present in the host genome. The polynucleotide sequences of the present invention, under the control of an operably linked promoter sequence, may further be flanked by additional sequences that advantageously affect its transcription and/or the stability of a resulting transcript. Such sequences are generally located upstream of the promoter and/or downstream of the 3′ end of the expression construct. The term “operably linked”, as used in reference to a regulatory sequence and a structural nucleotide sequence, means that the regulatory sequence causes regulated expression of the linked structural nucleotide sequence. “Regulatory sequences” or “control elements” refer to nucleotide sequences located upstream, within, or downstream of a structural nucleotide sequence, and which influence the timing and level or amount of transcription, RNA processing or stability, or translation of the associated structural nucleotide sequence. Regulatory sequences may include promoters, translation leader sequences, introns, enhancers, stem-loop structures, repressor binding sequences, termination sequences, pausing sequences, polyadenylation recognition sequences, and the like.

In another embodiment, the nucleotide sequence of the present invention comprises an inverted repeat sequence separated by a spacer sequence. The spacer sequence may be a region comprising any sequence of nucleotides that facilitates secondary structure formation between the inverted repeat sequences. In one embodiment, the spacer sequence is part of the sense or antisense polynucleotide sequence for mRNA. The spacer sequence may alternatively comprise any combination of nucleotides or homologues thereof that are capable of being linked covalently to a nucleic acid molecule. The spacer sequence may comprise a contiguous sequence of nucleotides of from about 8-100 nucleotides in length, or alternatively from about 100-200 nucleotides in length, or from about 200-400 nucleotides in length, or from about 400-500 nucleotides in length, or from about 500 to about 1500 nucleotides in length.

The gene or genes targeted for suppression may be amplified using any thermal amplification means and the precise nucleotide sequence determined. One skilled in the art is able to modify the thermal amplification conditions in order to ensure optimal amplicon product formation, and the amplicon may be used as a template for in vitro transcription to generate sense and antisense RNA with the included minimal promoters.

As used herein, the phrase “a substantially purified nucleic acid”, “an artificial sequence”, “an isolated and substantially purified nucleic acid”, or “an isolated and substantially purified nucleotide sequence”, with respect to a naturally occurring nucleotide sequence, refers to a nucleic acid molecule that is substantially removed from the composition with which it is associated in its natural state. Examples of a substantially purified nucleic acid molecule include: (1) a DNA sequence comprising the contiguous sequence at least about 17, or about 18, or about 19 or more nucleotides in length consisting of a portion of a naturally occurring DNA molecule, but which is not flanked by polynucleotide sequences occur naturally on either end of the contiguous sequence; (2) a nucleic acid molecule comprising a naturally occurring contiguous nucleotide sequence isolated from its naturally occurring state and incorporated into a DNA construct; (3) a cDNA, a genomic DNA fragment isolated and purified substantially from all other genomic DNA to which it was originally naturally associated, an amplicon fragment produced using thermal amplification procedures, or a restriction fragment; (4) recombinant DNA; and (5) synthetic DNA. A substantially purified nucleic acid may also be comprised of one or more segments of any of the sequences referred to hereinabove.

Nucleic acid molecules, fragments thereof, and complements thereof selected from the group consisting of SEQ ID NO:1-45568 may be employed as probes or primers to identify related nucleic acid molecules from other species for use in the present invention to produce desired dsRNA, siRNA, and miRNA molecules. Such related nucleic acid molecules include the nucleic acid molecules that encode the complete amino acid sequence of a protein, and the promoters and flanking sequences of such molecules. In addition, such related nucleic acid molecules include nucleic acid molecules that encode gene family members. Such molecules can be readily obtained by using the above-described nucleic acid molecules or fragments thereof to screen complementary DNA or genomic DNA libraries obtained from a nematode or other plant pest species. The screen can be any physical means such as northern, southern, or any immunologically based screening method that detects either the specific sequence of a nucleotide molecule, or the transcribed and/or translated product of such nucleotide molecule, or any mathematical algorithm that is used for comparing nucleotide sequences in silico.

Nucleic acid molecules, fragments thereof, and complements thereof selected from the group consisting of SEQ ID NO:45569-SEQ ID NO:97729 may also be used in a similar fashion to screen other genomes, libraries, and organisms for related sequences. Such related sequences are expected to include but not be limited to homologues that include nucleic acid molecules that encode, in whole or in part, protein homologues of other pest species, plants or other organisms. Such molecules can be readily obtained by using the above-described nucleic acid molecules or fragments thereof to screen EST, cDNA or gDNA libraries. Such homologous molecules may differ in their nucleotide sequences from those found in one or more of SEQ ID NO:1-SEQ ID NO:45568 and SEQ ID NO:45569 through SEQ ID NO:97729 or complements thereof, because perfect complementarity is not required for such related sequences to hybridize to each other. In a particular embodiment, methods for 3′ or 5′ RACE may be used to obtain such sequences (Frohman, M. A. et al., Proc. Natl. Acad. Sci. (U.S.A.) 85:8998-9002, 1988; Ohara, O. et al., Proc. Natl. Acad. Sci. (U.S.A.) 86:5673-5677, 1989). In general, any of the above described nucleic acid molecules or fragments may be used to generate dsRNA's, siRNA's, and/or siRNA's that are suitable for use in a diet, in a spray-on mix, or in a recombinant DNA construct of the present invention.

As used herein, the phrase “coding sequence”, “structural nucleotide sequence” or “structural nucleic acid molecule” refers to a polynucleotide molecule that is translated into a polypeptide when placed under the control of appropriate regulatory sequences. The structural nucleotide sequence, coding sequence, or structural nucleic acid molecule can be referred to using other terms in the art, but is intended to include DNA as well as RNA molecules. A coding sequence can include, but is not limited to, genomic DNA sequences or portions thereof identified to encode or to be capable of encoding a polypeptide, a cDNA produced as a result of reverse transcription of mRNA that has been purified substantially because if its ability to hybridize to a polyT sequence, expressed sequence tagged (EST) sequences, and recombinant nucleotide sequences produced specifically for expression of a protein sequence.

Two molecules are said to be “minimally complementary” if they can hybridize to one another with sufficient stability to permit them to remain annealed to one another under at least conventional “low-stringency” conditions. Similarly, the molecules are said to be complementary if they can hybridize to one another with sufficient stability to permit them to remain annealed to one another under conventional “high-stringency” conditions. Conventional stringency conditions are described by Sambrook, et al., (1985). Appropriate stringency conditions which promotes hybridization of two different nucleic acid sequences are, for example, incubation of the two sequences together in 6.0× sodium chloride/sodium citrate (SSC) at about 45° C. where one of the two different sequences is tethered in some fashion to a solid support and the untethered sequence is linked to a reporter molecule such as a ligand that can be detected using an immunological means, a fluorophores, a radioisotope, or an enzyme. The hybridization of the two sequences under the above conditions can be followed by a wash in 2.0×SSC at 50° C. to remove any excess reagents or unbound or unhybridized probe or untethered molecules (Current Protocols in Molecular Biology, John Wiley & Sons, N.Y. (1989), 6.3.1-6.3.6). For example, the salt concentration in the wash step can be selected from a low stringency of about 2.0×SSC at 50° C. to a high stringency of about 0.2×SSC at 50° C. In addition, the temperature in the wash step can be increased from low stringency conditions at room temperature (about 22° C.) to high stringency conditions (about 65° C.). Temperature and salt may be varied together or independent of each other.

A nucleic acid for use in the present invention may specifically hybridize to one or more of nucleic acid molecules from nematodes or complements thereof under moderately stringent conditions, for example at about 2.0×SSC and about 65° C. A nucleic acid for use in the present invention will include those nucleic acid molecules that specifically hybridize to one or more of the nucleic acid molecules disclosed therein as set forth in SEQ ID NO:1 through SEQ ID NO:47643 or complements thereof under high stringency conditions. Preferably, a nucleic acid for use in the present invention will exhibit at least from about 70%, at least from about 80%, at least from about 90%, at least from about 95%, at least from about 98% or even about 100% sequence identity with one or more nucleic acid molecules as set forth in SEQ ID NO:45569 through SEQ ID NO:47643.

Nucleic acids of the present invention may be entirely synthetically constructed or assembled piecemeal from naturally occurring or combinations of naturally occurring and synthetic components. All or any portion of the nucleic acids of the present invention may be synthesized without reference to codon usage calculated for any particular plant species, however when a particular sequence is intended to be effective in suppression of one or more genes in one or more pest species, it is preferable that the sequence be selected such that the sequence in any gene or species targeted for suppression be entirely or substantially entirely identical or entirely or substantially entirely complementary to the suppressor sequence.

The present invention also relates to recombinant DNA constructs for expression in a microorganism. Heterologous nucleic acids from which an RNA of interest is transcribed can be introduced into a microbial host cell, such as a bacterial cell or a fungal cell, in order to produce quantities of double stranded RNA for use in suppression of one or more genes in one or more plant pests.

The present invention also contemplates transformation of a polynucleotide sequence of the present invention into a plant to achieve nematode or other plant pest inhibitory levels of expression of one or more dsRNA molecules. A plant transformation vector comprises one or more nucleotide sequences that is/are capable of being transcribed as an RNA molecule and that is/are substantially homologous and/or complementary to one or more nucleotide sequences encoded by the genome of the nematode, or other plant pest, such that upon uptake of the RNA molecule, results in a down-regulation of expression of at least one of the respective nucleotide sequences of the nematode or other plant pest. In one embodiment the plant transformation vector is an isolated and purified DNA molecule comprising a promoter operatively linked to a contiguous nucleotide sequence comprising one or more polynucleotide molecules of the present invention selected from the group consisting of SEQ ID NO:45569 through SEQ ID NO:50775. The polynucleotide molecule includes a segment comprising all or part of a RNA molecule complementary to a targeted RNA within a nematode or pest cell, and may also contain a functional intron sequence positioned either upstream of or within the transcribed RNA sequence, and may also contain a five prime (5′) untranslated leader sequence (i.e., a UTR or 5′-UTR) positioned between the promoter and the point of transcription initiation.

A plant transformation vector may contain sequences for suppression of more than one gene, thus allowing production of more than one dsRNA for inhibiting expression of two or more genes. One skilled in the art will readily appreciate that segments of DNA whose sequence corresponds to that present in different genes can be combined into a single composite DNA segment for expression in a transgenic plant to achieve suppression of one or more nematode or pest genes, one or more plant genes, or a combination thereof. Alternatively, a plasmid of the present invention already containing at least one DNA segment can be modified by the sequential insertion of additional DNA segments between an enhancer and/or promoter and the terminator sequences. A nematode or other plant pest control agent of the present invention may be designed for the inhibition of multiple genes, and the genes to be inhibited can be obtained from the same nematode or other plant pest species in order to enhance the effectiveness of the pest control agent, or from different races/variants of the same pest species, or from different pest species or other organisms. In certain embodiments, the genes derived from different nematodes or other plant pests provide for a broadening of the range of nematodes and other plant pests against which the pest control agent is effective. When multiple genes in one pest are targeted for suppression, a polycistronic DNA element can be fabricated (Fillatti, US Patent Application Publication No. US 2004-0029283 A1).

A promoter that drives expression of a polynucleotide sequence in a particular species of plant is selected for use in expression constructs in which a nucleotide sequence of the present invention is to be used to transform a plant. Promoters that function in different plant species are known in the art. Promoters useful for expression of polypeptides in plants are those that are inducible, viral, synthetic, or constitutive as described in Odell et al. (1985 Nature 313:810-812), and/or promoters that are temporally regulated, spatially regulated, and spatio-temporally regulated. For the purpose of the present invention, e.g., for optimum control of species that feed on roots, it is preferable to achieve the highest levels of expression of these genes within the roots of plants. A number of promoters exhibiting root-enhanced levels of expression of operably linked sequences have been identified. (Lu et al., 2000 J. Plant Phys., 156(2):277-283; U.S. Pat. Nos. 5,837,848 and 6,489,542). Expression of the constructs of the present invention may preferably be from polymerase III promoters as an alternative to conventional polymerase II promoters, and also may be linked to inducible promoters, or heterologous promoters that require heterologous accessory proteins, such as for example, phage T7 promoters and the like. Promoters that are induced as a result of the establishment by a cyst nematode of a feeding site (feeding site specific promoters), and promoters up-regulated by nematode invasion are specifically contemplated for use in the present invention (Gheysen et al., 2002, Ann. Rev. Phytopathol. 40:191-219).

A recombinant DNA vector or construct of the present invention will typically comprise a marker that confers a selectable phenotype on transformed plant cells, and may also be used to select for plants or plant cells that contain the exogenous nucleic acids of the present invention. The marker may encode biocide resistance, antibiotic resistance (e.g., kanamycin, G418 bleomycin, hygromycin, etc.), or herbicide resistance (e.g., glyphosate, etc.). Examples of selectable markers include, but are not limited to, a neo gene (Potrykus et al., 1985 Mol. Gen. Genet. 199:183-188) which codes for kanamycin resistance and can be selected for using kanamycin, G418, etc.; a bar gene which codes for bialaphos resistance; a mutant EPSP synthase gene (Hinchee et al., 1988 Bio/Technology 6:915-922) which encodes glyphosate resistance; a nitrilase gene which confers resistance to bromoxynil (Stalker et al., 1988 J. Biol. Chem. 263:6310-6314); a mutant acetolactate synthase gene (ALS) which confers imidazolinone or sulphonylurea resistance (European Patent Application 154,204); an AMPA-acetyltransferase gene for resistance to phosphonates (U.S. Pat. No. 6,448,476), a methotrexate resistant DHFR gene (Thillet et al., 1988 J. Biol. Chem. 263:12500-12508), and compositions for chloroplast or plastid transformation selection (U.S. Pat. Nos. 5,693,507, 5,451,513, and WO 95/24492).

A recombinant vector or construct of the present invention may also include a screenable marker for monitoring expression. Exemplary screenable markers include a β-glucuronidase or uidA gene (GUS) which encodes an enzyme for which various chromogenic substrates are known (Jefferson, Plant Mol. Biol, Rep. 5.387-405, 1987; Jefferson et al., EMBO J. 6:3901-3907, 1987); an R-locus gene, which encodes a product that regulates the production of anthocyanin pigments (red color) in plant tissues (Dellaporta et al., Stadler Symposium 11:263-282, 1988); a β-lactamase gene (Sutcliffe et al., Proc. Natl. Acad. Sci. (U.S.A.) 75:3737-3741, 1978), a gene which encodes an enzyme for which various chromogenic substrates are known (e.g., PADAC, a chromogenic cephalosporin); a luciferase gene (Ow et al., Science 234:856-859, 1986) a xylE gene (Zukowsky et al., Proc. Natl. Acad. Sci. (U.S.A.) 80:1101-1105, 1983) which encodes a catechol dioxygenase that can convert chromogenic catechols; an α-amylase gene (Ikatu et al., Bio/Technol. 8:241-242, 1990); a tyrosinase gene (Katz et al., J. Gen. Microbiol. 129:2703-2714, 1983) which encodes an enzyme capable of oxidizing tyrosine to DOPA and dopaquinone which in turn condenses to melanin; an α-galactosidase, which catalyzes a chromogenic α-galactose substrate; and a β-galactosidase which catalyzes the conversion of a chromogenic β-galactoside substrate.

In general a functional recombinant DNA is introduced at a non-specific location in a plant genome. In special cases it may be useful to insert a recombinant DNA construct by site-specific integration. Several site-specific recombination systems exist which are known to function implants include cre-lox as disclosed in U.S. Pat. No. 4,959,317 and FLP-FRT as disclosed in U.S. Pat. No. 5,527,695.

Preferred plant transformation vectors include those derived from a Ti plasmid of Agrobacterium tumefaciens (e.g. U.S. Pat. Nos. 4,536,475, 4,693,977, 4,886,937, 5,501,967 and European Patent Application No. 0122791). Agrobacterium rhizogenes plasmids (or “Ri”) are also useful. Other preferred plant transformation vectors include those disclosed, e.g., by Herrera-Estrella (1983 Nature 303:209-213), Bevan (1983 Nature 304:184-187), Klee (1985 Bio/Technol. 3:637-642) and Eur. Pat Appl. No. EP0 120 516.

Methods and compositions for transforming plants by introducing a recombinant DNA construct into a plant genome includes any of a number of methods known in the art. One method for constructing transformed plants is microprojectile bombardment as illustrated in U.S. Pat. Nos. 5,015,580 (soy), 5,550,318 (corn), 5,538,880 (corn), 6,153,812 (wheat), 6,160,208 (corn), 6,288,312 (rice) and 6,399,861 (corn). Another method for constructing transformed plants is Agrobacterium-mediated transformation in cotton (U.S. Pat. No. 5,159,135), corn (U.S. Pat. No. 5,591,616), and soy (U.S. Pat. Nos. 5,824,877 & 6,384,301).

The term “transgenic plant cell” or “transgenic plant” refers to a plant cell or a plant that contains an exogenous or heterologous polynucleotide sequence. A transgenic plant also comprises progeny (seeds, and plants and seeds produced from such seeds, etc.) of any generation of such a transgenic plant or a seed of any generation of all such transgenic plants wherein said progeny or seed comprises the exogenous or heterologous polynucleotide sequence. The heterologous or polynucleotide sequence is a DNA molecule that is transcribed into the RNA, sRNA, dsRNA, siRNA, or miRNA or fragment thereof of the present invention.

A transgenic plant formed using Agrobacterium mediated transformation methods contains at least a single recombinant DNA sequence inserted into the plant chromosome and is referred to as a transgenic event. Such transgenic plants are referred to as being heterozygous for the inserted exogenous sequence. A transgenic plant homozygous with respect to a transgene can be obtained by sexually mating (selfing) an independent segregant transgenic plant that contains a single exogenous gene sequence to itself, for example an F0 plant, to produce F1 seed. One fourth of the F1 seed produced will be homozygous with respect to the transgene. F1 seed can be tested using a SNP or related thermal amplification assay that allows distinction between heterozygotes and homozygotes (i.e., a zygosity assay).

Transgenic plants can also be prepared by crossing a first plant having a recombinant DNA construct with a second plant lacking the construct. For example, a recombinant DNA designed for targeting the suppression of a target gene can be introduced into a first plant line to produce a transgenic plant which can be crossed with a second plant line to introgress the recombinant gene suppression DNA into the second plant line. The second plant line may already contain or be later transformed or bred with another transgenic line to contain one or more transgenes that are different from the gene suppression construct being introgressed from the first plant line.

Without intending to be limited to any single embodiment, the nucleotide sequences of the present invention exhibit a wide variety of usefulness. For example, the sequences can be used to synthesize dsRNA molecules either in in vivo or in vitro systems selected for their ability to cause gene suppression and therefore pest inhibition and such moleculed can be expressed in a transgenic plant, applied to the rhizosphere or biosphere of a plant, or applied in a seed coating or treatment for causing gene suppression in a pest. The sequences can be used in kits incorporating methods for detecting DNA, RNA, or siRNA's in a seed, plant, tissue, biological sample, meal, oil, flour, food product, commodity product, and the like. The sequences can be used for detecting the presence of a homologous sequence in a biological sample. The sequences can be used to construct a dsRNA for suppression of a target gene and can be linked to an RNA segment that binds specifically to one or more receptor molecules, bringing the dsRNA segment into close proximity to a membrane surface, and increasing its likelihood of being taken up by a cell which contains a gene that is targeted for suppression by the dsRNA.

In one embodiment, a nucleotide sequence of the present invention can be recorded on one or more computer readable media. As used herein, “computer readable media” refers to any tangible medium of expression that can be read and accessed directly by a computer. Such media include, but are not limited to: magnetic storage media, such as floppy discs, hard discs, and magnetic tape. Optical storage media include physical storage devices such as compact diskettes. Electrical storage media include random access and read only memory devices (RAM and ROM). A skilled artisan can readily appreciate that any of the presently known computer readable mediums can be used to create a manufacture comprising a computer readable medium having recorded thereon one or more sequences of the present invention. These devices can be accessed with a computer and used to perform a search and comparison of any other sequence of like composition (i.e., nucleotide sequences compared to nucleotide sequences, amino acid sequences compared to amino acid sequences, etc) to determine whether and to what extent a similarity or identity is present between the sequences being compared.

As used herein, “recorded” refers to a process for storing information on computer readable medium. A skilled artisan can readily adopt any of the presently known methods for recording information on computer readable medium to generate media comprising the nucleotide sequence information of the present invention. A variety of data storage structures are available for creating a computer readable medium having recorded thereon one or more sequences of the present invention. The choice of the data storage structure will generally be based on the means chosen to access the stored information. In addition, a variety of data processor programs and formats can be used to store the sequence information of the present invention on computer readable medium. The sequence information can be represented in a word processing text file, formatted in commercially available software such as WordPerfect, Microsoft Word, or shareware such as Linux, or represented in the form of an ASCII text file, stored in a database application, such as DB2, Sybase, Oracle, or the like. The skilled artisan can readily adapt any number of data processor structuring formats (e.g. text file or database) in order to obtain computer readable medium having recorded thereon the nucleotide sequence information of the present invention.

Computer software is publicly available which allows a skilled artisan to access sequence information provided in a computer readable medium. Software which implements the BLAST (Altschul et al., J. Mol. Biol. 215: 403-410, 1990) and BLAZE (Brutlag, et al., Comp. Chem. 17: 203-207, 1993) search algorithms on a Sybase system can be used to identify open reading frames (ORFs) within sequences such as the EST's that are provided herein and that contain homology to ORFs or proteins from other organisms. Such ORFs are protein-encoding fragments within the sequences of the present invention and are useful in producing commercially important proteins such as enzymes used in amino acid biosynthesis, metabolism, transcription, translation, RNA processing, nucleic acid and a protein degradation, protein modification, and DNA replication, restriction, modification, recombination, and repair.

EXAMPLES
Example 1

This Example illustrates the construction and DNA sequence analysis of SCN genome libraries.

SCN genomic DNA libraries (LIB5513, LIB 5514, LIB5519, and LIB5520) were constructed from SCN strain OP25 genomic DNA (Dong et al., 1997, Genetics 146:1311-1318). The libraries were generated by ligating size-selected physically sheared DNA into the high copy number plasmid pUC18 and the resulting ligation mixture was transformed into E. coli by electroporation. 10 micrograms of SCN genomic DNA were resuspended into 30 microliters TE buffer. The DNA was sheared by sonication. The sonicated DNA was 5′ end-repaired using T4 DNA polymerase (New England BioLabs) and 10 mM dNTP's in a total reaction volume of 35 microliters and equilibrated to 1× ligation buffer (New England BioLabs). 3′ overhangs were repaired by treatment with T4 polynucleotide kinase. The mixture was incubated at 15° C. for 20 minutes, and transferred to 65° C. for 15 minutes to inactivate the kinase and polymerase, and incubated at room temperature for an additional 10 minutes. The repaired DNA was size fractionated by electrophoresis in a 0.7% agarose gel adjacent to a 1 Kb molecular weight marker at 80 volts for two hours in TBE buffer. The 2-4 KB and 4-8 KB DNA fragments were excised from the agarose gel and transferred into microcentrifuge tubes. The size-selected DNA fragments were isolated from the agarose gel and a second round of size selection was performed to eliminate small DNA fragments co-migrating with the selected range in first gel fractionation. Approximately 100 nanograms of the size-selected repaired DNA was inserted by ligation into a pUC18-HincII digested vector (molar ratio of 5 to 1). The ligated DNA was transformed into E. coli DH10B cells by electroporation and planted to LB plates containing 100 micrograms per milliliter ampicillin and incubated for 18-24 hours at 37 C. Several colonies that arose after incubation were randomly selected. The colonies were tested to determine the average DNA insert size and the average number of colonies in the library that appeared to contain no inserted recombinant DNA. Four libraries were constructed. The average insert size in library LIB5513 was 2-4 KB, in library LIB5514 was 4-8 KB, in library LIB5519 was 2-4 KB, and in library LIB5520 was 4-8 KB. Samples of each library were collected and combined together and deposited with the American Type Culture Collection (ATCC) at Rockville Md., USA on Feb. 15, 2005. The combined library was submitted to ATCC, designated as LIB5513_—14_—19_—20, and the ATCC has assigned the patent deposit number PTA-6583 to the deposited material.

The cells of the libraries were then plated on large bioassay plates containing Luria Broth (Difco) supplemented with 100 microgram/milliliter carbenicillin (ICN Biomedical), 64 microgram/milliliter IPTG (Shelton Scientific) and 80 microgram/milliliter X-Gal (Shelton Scientific). Individual blue transformants were then picked into 1.2 ml Terrific Broth (Difco) supplemented with 125 μg/ml Ampicillin (Calbiochem) in 96 deep-well boxes by Genetix Q-bot. The boxes were incubated for 21 hours at 37° C., each well archived to individual wells in 384-well glycerol plates, and then pelleted and stored at −20 C.

Alkaline lysis DNA extraction was performed on samples of pelleted clones using a QUIAGEN bead based platform on an automated robotic preparation system. Eluted DNA was stored for sequencing at 4° C. in a 96-well COSTAR plate. Two microliters of the DNA solution was then transferred into a 384 well microtiter plate (AXYGEN) using a Hamilton MPH96 Pipetting Robot. The pipetted DNA was then denatured for 5 minutes at 95° C., and two microliters of Big Dye Reaction Mix (Big Dye Terminators v3.0, 3.2 pmol sequencing primer, 1×TNK, and 0.5M MgCl₂) was then added to the denatured DNA using a Hamilton MPH96 Pipetting Robot. Each clone was sequenced using M13 forward and reverse primers in a PCR sequencing reaction using the conditions as follows: 95° C. for 5 seconds, 45° C. for 5 seconds, 60° C. for 2 minutes 30 seconds for a total of 25 cycles. The sequencing reactions were ethanol precipitated and re-suspended in water and loaded onto an ABI 3730×l Sequencing Analyzer (APPLIED BIOSYSTEMS) to generate sequence trace data for each sample. Approximately 400,000 sequencing reads were generated from the four Heterodera glycines genome libraries.

Example 2

This Example illustrates the analysis, characterization, and assembly of the sequences obtained from DNA sequence analysis of the SCN genome libraries.

The sequence trace data was converted to sequence and quality files and standard quality control procedures were applied through the use of the block 0/1 pipelines. Quality control procedures included sequence quality trimming, sequence identity, cloning sequence removal, and contamination identification and removal. The results of the general sequencing pre-processing steps were stored in the sequence database SeqDB. Data passing quality controls were retrieved for inclusion in the assembly step. The dataset to be assembled consisted of 338,266 sequence reads that passed the block 0/1 process, represented by an initial output of 404,372 sequencing reads that were submitted to the block 0/1 process. A file of clone pair constraints was produced on the basis of known clone naming conventions and library construction details (insert size range). The clone pair constraint file consisted of 159,389 pairwise entries. Fasta, quality, and constraint files were used as input to the PCAP program (Version Date: Sep. 3, 2004, Huang, X., Wang, J., Aluru, S., Yang, S.-P. and Hillier, L. (2003): PCAP: A Whole-Genome Assembly Program. Genome Research, 13: 2164-2170), and the sequences were assembled. 45,568 output genomic contig sequences were produced whose sum length represented about 80.8 Million bases. These contig sequences are represented by the sequences as set forth in SEQ ID NO:1-SEQ ID NO:45568 and were subsequently used as input sequences to define generic regions of the SCN genome sequence corresponding to predicted coding sequences (referred to herein as vcDNA's or virtual complementary DNA's) and predicted promoter and intronic sequences.

SCN expressed sequences were collected from public sources and used to compare the genomic sequences identified herein as well as to identify unique sequences not present in any known public database set. Public sequences were collected into a file which contained non-identical contigs from (1) the Genome Sequencing Center at Washington University in St. Louis, Mo., USA (Nemagene clusters; McCarter et al., 2003, J. Nematology 35:465-469), (2) Parkinson contigs (Nembase clusters; Parkinson et al., 2004, Nature Genetics 36:1259-1267), (3) EST's in GenBank not contained in contigs (singletons), and (4) nucleotide sequences representing non-EST DNA sequences in GenBank (e.g., mRNAs). These sequences were compiled into and referred to herein as an essential gene sequence list corresponding to sequences as set forth herein at SEQ ID NO:47644-SEQ ID NO:50775.

Gene finding results were consolidated in a relational database in such a way that each predicted gene is represented by a set of coordinates that define the position of all segments of the gene on the genomic DNA contig (gDNA). The genes are described herein, and in particular in the Feature Fields of the Sequence Listing with reference to the nucleotide positions of each vcDNA giving rise to an amino acid sequence and in the amino acid sequence SEQ ID NO's as nucleotide sequences corresponding to portions of vcDNA's encoding the amino acid sequence. Sequences between the indicated protein coding portions correspond to predicted intronic sequences. Other sequence segments that are represented at least in the genomic sequences set forth in SEQ ID NO:1-SEQ ID NO:45568 include but are not limited to peptide-encoding segments such as initial exon, internal exon, terminal exon, or single exon and the like, and non-coding segments including promoter regions, transcription initiation sequences, transcription termination sequences, and polyadenylation signal sequences, and the like. Often the same position within a gDNA contig is predicted to contain a gene by more than one gene finding program. Thus, in order to prepare a library of genes where each position (locus) of the genome is represented by a single gene, several different gene prediction methods were applied and the results were consolidated according to the following algorithm.

1. For each gDNA contig, all clusters of overlapping genes were defined. Each cluster was assumed to correspond to a single gene. The cluster was defined as a set of sequences located on the same DNA strand and either predicted to overlap based on nucleotide sequence identity along the lengths of the sequences or predicted to be located closer than 50 nucleotides from each other. Only peptide-encoding segments were considered when defining a cluster. The start and end positions of the cluster define the maximal dimension of the gene.

2. For each cluster the preferred gene was selected, which represents this locus in the library. The selection algorithm is described as follows:

(a) All genes in a cluster were ranked by the gene-prediction method that produced them. The ranking by the different methods was intended to describe assumed accuracy of the method in predicting genes. The ranking was ordered arbitrarily using FgeneSH, Genemark.hmm and AAT/NAP data results. The AAT/GAP results were not ranked at all, but were used only if there were no other prediction for the locus, i.e. cluster contained only gene(s) predicted by AAT/GAP.

(b) The highest ranking gene was selected unless there were several equally ranked genes (i.e. predicted by the same method) or the cluster coverage by this gene was below 60%. The cluster coverage was computed as the ratio of the gene length to the length of the cluster (maximal dimension of the gene).

(d) If the cluster coverage for the best-ranking gene was below 60%, the lower ranking genes were considered (in the ranking order) and the first one providing a gain in cluster coverage of at least 10% was selected.

(e) If a cluster contained only AAT/GAP-predicted genes—the one with the best cluster coverage was selected.

(f) For all other clusters, additional filtering was completed—only sequences that exhibited a translation product of at least 16 amino acids in length were selected.

(g) If a cluster contained only Genemark.hmm-predicted genes—no gene was selected and the locus was assumed not to contain any gene.

The method described above resulted in a list of “preferred” genes. The actual DNA sequence for each of these genes was prepared by extracting a subsequence (region of a sequence) of a gDNA contig which corresponded to the coordinates of the gene. The sequences prepared contained all predicted exons and introns of the gene. In the case of MT/GAP and FgeneSH genes they also may contain regions between transcription and translation initiation sequences, and between translation termination and polyadenylation sequences.

The three gene-predicting programs—FgeneSH, Genemark.hmm and AAT/NAP—in addition to predicting positions of genes, also predict sequences of the translation product, if any. Thus, the “preferred” genes and their translated peptide sequences were simultaneously predicted by these methods. Virtual cDNA sequences (vcDNA) were prepared from genes derived only from AAT/GAP prediction results by extracting regions of genomic DNA (gDNA) corresponding to the predicted exons and splicing them together. These virtual cDNA sequences were translated using a translator tool. The feature fields of indicated peptide SEQ ID NO's identify genomic contig sequence positions (for example, Contig_ID=SeqID_XXX) for the coding sequence contained therein. Additional information provided in the feature fields includes the identity of SCN-specific sequences, the nucleotide positions of these sequences in the vcDNA sequence, homology to existing sequences in publicly available databases, a numerical evaluation of the extent of the homology, and the predicted function if any associated with the peptide.

The vcDNA sequences were used to identify sequences corresponding to SCN specific promoter sequences using the following procedure:

1. For each gene predicted by either of the FgeneSH, Genemark.hmm or AAT/NAP prediction algorithms, the position of the first peptide-encoding segment was used as the reference point for sequence extraction. The sequence of the gDNA contig which starts 1000 nucleotides upstream and ends 2 nucleotides downstream of the reference point was extracted.

2. The resulting sequence of the upstream region was shorter if the gene was located closer than 1000 nucleotides to the end of the genomic contig. If there was another gene located upstream and predicted by one of these methods—FgeneSH, Genemark.hmm or AAT/NAP, the upstream region was shortened (truncated) so that it did not overlap with the closest peptide-encoding segment of that gene. If the resulting sequence was shorter than 50 nucleotides, it was not included as a promoter sequence in the library of promoter sequences.

3. If the resulting sequence did not end with the translation initiation codon ATG, i.e., the predicted gene was not N-terminal complete—then the sequence was not included as a promoter sequence in the library of promoter sequences.

4. Sequences located upstream of AAT/GAP-predicted genes were not included in the library of promoter sequences since this program did not predict a translation initiation position and in certain situations placed the predicted gene on the wrong strand of a gDNA contig.

Example 3

This Example illustrates the annotation of predicted SCN genes.

Two methodologies were used to provide annotations of the predicted Heterodera glycines (SCN) peptides, including Gene ontology (GO) and SmartBlast. Both GO and SmartBlast procedures were developed through homology-based sequence searches. In GO procedures, the peptide sequences from SCN peptides were used to BLAST against a protein sequence database, for example, the non-redundant protein (nr-aa) database maintained by the National Center for Biotechnology Information as part of GenBank. The highly conserved homologues of nr-aa from a variety of species were further selected with a minimal E value of 1E-08. The selected SCN homologues were subjected to the sequence match with a protein sequence database (GO proteins from GO Ontology consortium). Finally, three categories according to the GO Ontology consortium (molecular function; biological process; and cellular component) were used to annotate the SCN sequences. In SmartBlast procedures, the peptide sequences from SCN peptides were also used to blast against the non-redundant protein as described above. The homologues were also selected with a minimal E value of 1E-08. Those homologues were subjected to filtering using some non-meaningful words, such as “putative”. The best meaningful homologues were used for SCN sequence annotation. The conditions used to provide the homolog annotation and the best hit with respect to any predicted SCN gene product were referred to in one or more of the feature fields for each of the SCN protein sequences selected from the group consisting of SEQ ID NO:119146-SEQ ID NO:121220, and were further identified as to molecular function, enzyme activity, cellular component and biological process. Genes characterized as encoding proteins that may be essential for survival based on the proteins' relationship at least to one or more C. elegans homologs and the phenotype of the knockout of the C. elegans homolog were further identified in one or more of the feature fields of each of the peptide sequences. The phenotype observed, abbreviations for each, and the standard nomenclature assigned for each with reference to that same phenotype and nomenclature in C. elegans was identified previously hereinabove.

Example 4

This Example illustrates a method for screening the SCN genome sequences, the predicted vcDNA sequences, and the predicted amino acid sequence encoded therefrom, against other sequences and selecting sequences unique to SCN.

The sequences disclosed herein can be used in a method to provide a DNA construct for expression of a dsRNA that is effective for silencing of a gene in a soybean cyst nematode or other plant pest by expressing such DNA construct in the cells of a transgenic plant and providing the plant in the diet of the nematode or pest. DNA sequences can be selected from the sequences of the present invention that are useful in achieving dsRNA-mediated gene silencing by selecting from a target gene a DNA sequence consisting of at least from about 17 to about 21 or more contiguous nucleotides. Effective short interfering RNA's (siRNAs) for gene repression are normally from about 21 to about 23-nt long double-stranded RNA duplexes. These siRNA's are known to incorporate into the RNA-inducing silencing complex (RISC). Once unwound, the single-stranded antisense strand guides RISC to the target mRNA, and induces the cleavage of the target messages, resulting in translational inhibition (Dykxhoorn, et al. Molecular Cell Biology, 4:457-467, 2003). Plant siRNA sequences have been characterized generally as contiguous nucleotide sequences of from about 24 nucleotides in length (Tang, 2003, Genes & Development 17:49-63). It is preferred that interfering RNA molecules are selected from the sequences as set forth in SEQ ID NO:1-SEQ ID NO:97729 to limit the un-intended “off-target” effect of gene repression by limiting the potential base-pairing with unintended targets of the host or other non-target organisms.

Example 5

This example illustrates the identification of SCN genes that can be targeted for suppression using the nucleotide sequences of the present invention.

A comparison of the SCN genes was made to the genes identified in C. elegans for which knockouts have been previously identified to result in an observable phenotype. RNAi phenotypes include maternal sterile, embryonic lethal and a variety of postembryonic phenotypes. The relationship between C. elegans knockout phenotypes and their protein sequences were obtained. These protein sequences were then compared to the protein sequences translated from the SCN genomic sequences of the present invention.

A BLAST searchable “All Protein Database” was constructed, which was composed of genome-wise SCN peptides and C. elegans proteins. A reciprocal blast procedure was used to identify the possible orthologues of C. elegans for each SCN peptide.

The All Protein Database was queried using protein sequences of the SCN peptides using the “blastp” algorithm with an E-value cutoff of 1e-8. Up to 1000 hits were retained for each SCN peptide used in the query, and separated by organism names, either C. elegans or SCN. For C. elegans, a list was retained for the hits with SCN sequences exhibiting a more significant E-value than the best hit of the organism. The list contains likely duplicated SCN genes, and was referred to as a Core List. Another list was retained for all the hits from each organism, sorted by the E-value, and was referred to as a Hit List. The hit was identified as an orthologue of the query sequence if it was within the Core List.

Knockout phenotypes of SCN were inferred according to the degree of evolutionary relationship determined to exist between SCN and C. elegans proteins with reference to the knockout phenotypes of C. elegans genes, referred to herein above. For example, C. elegans C37H5.8 corresponds to a HSP-6 protein, and a knockout of this gene has been associated with the observed phenotypes of embryonic lethality and larval arrest. Orthologue identification from the above query indicated that an SCN amino acid sequence corresponding to SEQ ID NO:119310 is an orthologue of C37H5.8. Therefore, it is believed that because of the relationship of the SCN sequence corresponding to SEQ ID NO:119310 to the C. elegans orthologue C37H5.8, suppression of the SCN gene corresponding to SCN vcDNA sequence as set forth at SEQ ID NO:45733 encoding the C37H5.8 orthologue at SEQ ID NO:119310 would be expected to result in an observable phenotype corresponding to embryonic lethal and/or larva arrest in SCN. SCN genes have been categorized based on their relationship to identifiable orthologues with genes or sequences in other organisms and some are further identified as essential genes. Such information has been provided for each amino acid sequence predicted from the vcDNA sequences and is listed in the feature fields for each sequence in the sequence listing. The feature field in the sequence listing has been used to identify important features of the DNA molecules of the present invention. A DNA construct that contains target sequences from multiple SCN essential genes can be constructed to express a chimeric dsRNA molecule that affects more than one SCN gene. This aspect of the present invention reduces the possibility of selecting for a population of SCN that is unaffected by the dsRNA molecule.

SCN genes were grouped into Pfam protein families. Pfam is a comprehensive database of protein domain families, based on multiple alignments of protein domains or conserved protein regions (Nucleic Acids Research 2004 32:D138-D141; Proteins 28:405-420, 1997.). Peptide sequences of a subset of SCN genes have been matched to Pfam entries with HMMPFAM program, with an expectation value cutoff of 0.1 (Biological sequence analysis: probabilistic models of proteins and nucleic acids, Cambridge University Press, 1998.) The subset included 5207 SCN protein sequences that were analyzed by this method, and 3397 of the 5207 protein sequences were grouped into 909 families, as set forth in Table 1.

In order to target a protein gene family for suppression with a single dsRNA molecule, it may be necessary to identify conserved DNA sequence regions among protein gene family members. After the amino acid sequence translations from the virtual cDNA sequences were grouped into protein families, the conserved sequence regions were identified through multiple sequence alignment of the DNA sequences of the family members. For example, using the program CLSUTALW (ref. Nucleic Acids Res. 22:4673-4680), member sequences of a Pfam group can be aligned. One example is illustrated by an alignment of SEQ ID NO's representative of the nucleotide sequences encoding the protein family members in the MRP_L47 family, a mitochondrial ribosomal protein family, corresponding to SEQ ID NO:49132 (HG02471), SEQ ID NO:50709 (HGC08009), and SEQ ID NO:46538 (HG2_—27019.C1.o1.np). An alignment of these three sequences allows the identification of conserved contiguous residues present in each of the three sequences. The conserved segments consisting of at least 21 contiguous nucleotides are representative of the preferred polynucleotide regions for expression in a double stranded RNA sequence for use in targeting the suppression of each member of the entire gene family. The comparison of protein sequences of family members identified and grouped in Table 1 enables the identification of related polynucleotide regions common among the family members by locating the corresponding cDNA and genomic contig sequences identified in the feature field of the Sequence Listing. Using this method of comparison, the protein sequences of family members identified in Table 1 and in SEQ ID NO:119146-SEQ ID NO:124352 allows the skilled artisan to identify the related polynucleotide regions that are common among the family members by locating the corresponding virtual cDNA (vcDNA) and genomic contiguous sequences as set forth in SEQ ID NO:1-SEQ ID NO:119145. These sequences can then be used in a DNA construct to express a dsRNA molecule in plant cells that is directed to the suppression of one or more genes in any of one or more plant pests. These polynucleotides can then be used in a DNA construct to express a homologous dsRNA molecule in plant cells.

TABLE 1

SCN GENE FAMILIES and ANNOTATIONS

SCN gene families

Gene name
SCN gene family members
Protein annotation

bZIP_1
SeqID_122625 SeqID_119811
bZIP transcription factor

SeqID_124042 SeqID_124331

SeqID_121799

Mito_carr
SeqID_121264 SeqID_122047
Mitochondrial carrier protein

SeqID_122051 SeqID_122111

SeqID_122259 SeqID_122457

SeqID_122504 SeqID_122594

SeqID_121347 SeqID_121360

SeqID_121361 SeqID_122965

SeqID_122993 SeqID_123057

SeqID_123135 SeqID_123186

SeqID_123212 SeqID_123240

SeqID_123284 SeqID_123348

SeqID_123379 SeqID_123391

SeqID_123930 SeqID_119420

SeqID_119539 SeqID_120127

SeqID_120194 SeqID_120280

SeqID_121563 SeqID_121585

SeqID_120988 SeqID_121115

SeqID_123589 SeqID_123608

SeqID_123626 SeqID_123732

SeqID_123814 SeqID_123863

SeqID_121678 SeqID_124088

SeqID_124151 SeqID_124350

SeqID_121751 SeqID_121752

SeqID_121870

bZIP_2
SeqID_121260 SeqID_122546
Basic region leucine zipper

SeqID_122625 SeqID_119811

SeqID_124042 SeqID_121799

Sec7
SeqID_120412
Sec7 domain

MutS_IV
SeqID_119581
MutS family domain IV

CtaG_Cox11
SeqID_119768
Cytochrome c oxidase assembly

protein Cta

Synaptobrevin
SeqID_122614 SeqID_122853
Synaptobrevin

SeqID_123133 SeqID_123159

SeqID_123266 SeqID_120966

SeqID_121065 SeqID_123723

Fer2
SeqID_122093 SeqID_122357
2Fe—2S iron-sulfur cluster binding

SeqID_119370 SeqID_124223
domain

SeqID_124309

WD40
SeqID_121225 SeqID_121265
WD domain, G-beta repeat

SeqID_122030 SeqID_122050

SeqID_122140 SeqID_122192

SeqID_122212 SeqID_122243

SeqID_122288 SeqID_122350

SeqID_122349 SeqID_122434

SeqID_122449 SeqID_122509

SeqID_122514 SeqID_122539

SeqID_122547 SeqID_121310

SeqID_121337 SeqID_121375

SeqID_122883 SeqID_122959

SeqID_122996 SeqID_123045

SeqID_123090 SeqID_123107

SeqID_123122 SeqID_123198

SeqID_123318 SeqID_123329

SeqID_121391 SeqID_119248

SeqID_119272 SeqID_119279

SeqID_119343 SeqID_119431

SeqID_119538 SeqID_119627

SeqID_119670 SeqID_119769

SeqID_119826 SeqID_119840

SeqID_119913 SeqID_119990

SeqID_121523 SeqID_120032

SeqID_120112 SeqID_120134

SeqID_120235 SeqID_120302

SeqID_120344 SeqID_120362

SeqID_120457 SeqID_120458

SeqID_120507 SeqID_120508

SeqID_120576 SeqID_120747

SeqID_120826 SeqID_120866

SeqID_120885 SeqID_120945

SeqID_120994 SeqID_120997

SeqID_121007 SeqID_121094

SeqID_121096 SeqID_121116

SeqID_121184 SeqID_121199

SeqID_123563 SeqID_123612

SeqID_123643 SeqID_123841

SeqID_123880 SeqID_121640

SeqID_123965 SeqID_124104

SeqID_124118 SeqID_124150

SeqID_124186 SeqID_124334

SeqID_121790 SeqID_121804

SeqID_121816 SeqID_121840

SeqID_121844

Skp1
SeqID_121262 SeqID_122339
Skp1 family, dimerisation domain

SeqID_121318 SeqID_119848

Fer4
SeqID_122076 SeqID_122198
4Fe—4S binding domain

SeqID_120449 SeqID_120455

SeqID_124131

Enolase_C
SeqID_122156 SeqID_123621
Enolase, C-terminal TIM barrel

domain

Mucin
SeqID_120313
Mucin-like glycoprotein

NHL
SeqID_121326
NHL repeat

FAT
SeqID_122973 SeqID_120212
FAT domain

Iso_dh
SeqID_122456 SeqID_120630
Isocitrate/isopropylmalate

SeqID_123561
dehydrogenase

APH
SeqID_122384 SeqID_122682
Phosphotransferase enzyme

SeqID_121416 SeqID_119410
family

SeqID_119453 SeqID_119764

SeqID_121622 SeqID_120808

SeqID_124140 SeqID_121803

Suf
SeqID_122937
Suppressor of forked protein (Suf)

Enolase_N
SeqID_122156 SeqID_123621
Enolase, N-terminal domain

Ldh_1_C
SeqID_122492 SeqID_120389
lactate/malate dehydrogenase,

SeqID_123782
alpha/beta C-t

HMG_CoA_synt
SeqID_122417 SeqID_120809
Hydroxymethylglutaryl-coenzyme

SeqID_121837
A synthas

PLDc
SeqID_119325 SeqID_120704
Phospholipase D Active site motif

Glycos_transf_1
SeqID_121164
Glycosyl transferases group 1

Dala_Dala_lig_C
SeqID_119260
D-ala D-ala ligase C-terminus

Kunitz_BPTI
SeqID_122170 SeqID_119622
Kunitz/Bovine pancreatic trypsin

SeqID_119623
inhibito

Nuc_sug_transp
SeqID_119240
Nucleotide-sugar transporter

cobW
SeqID_122575
CobW/HypB/UreG, nucleotide-

binding domain

L15
SeqID_121270 SeqID_122374
Ribosomal protein L15

SeqID_123713

Ldh_1_N
SeqID_122492 SeqID_121371
lactate/malate dehydrogenase,

SeqID_122868 SeqID_120389
NAD binding do

SeqID_123782

CLP_protease
SeqID_122193 SeqID_122948
Clp protease

SeqID_124275 SeqID_120143

SeqID_123619 SeqID_121952

HEAT_PBS
SeqID_122486 SeqID_120345
PBS lyase HEAT-like repeat

SeqID_123693

NIC
SeqID_121904
Nucleoporin interacting component

SecY
SeqID_122609 SeqID_123948
eubacterial secY protein

PCI
SeqID_122070 SeqID_122383
PCI domain

SeqID_119309 SeqID_119633

SeqID_120088 SeqID_120892

SeqID_123711 SeqID_123975

SeqID_121783

Abhydro_lipase
SeqID_119405
ab-hydrolase associated lipase

region

Mra1
SeqID_123459
Suppressor Mra1

tRNA-synt_1b
SeqID_123017 SeqID_120260
tRNA synthetases class I (W and

SeqID_121648
Y)

NIF
SeqID_121832
NLI interacting factor-like

phosphatase

Laminin_G_1
SeqID_120488
Laminin G domain

tRNA-synt_1c
SeqID_122217 SeqID_122354
tRNA synthetases class I (E and

SeqID_123425 SeqID_119559
Q), cata

SeqID_120889 SeqID_123797

Laminin_G_2
SeqID_120488
Laminin G domain

tRNA-synt_1e
SeqID_119570 SeqID_119679
tRNA synthetases class I (C)

catalytic d

Acyl-CoA_dh_M
SeqID_123412 SeqID_119407
Acyl-CoA dehydrogenase, middle

SeqID_119554
domain

Mak16
SeqID_123061 SeqID_120231
Mak16 protein

SeqID_120297

Clp1
SeqID_120900
Pre-mRNA cleavage complex II

protein Clp1

Guanylate_cyc
SeqID_119460
Adenylate and Guanylate cyclase

catalyst

Acyl-CoA_dh_N
SeqID_122997 SeqID_123412
Acyl-CoA dehydrogenase, N-

SeqID_119407 SeqID_119740
terminal doma

SeqID_124296

RNase_PH_C
SeqID_122716 SeqID_120146
3′ exoribonuclease family, domain 2

MoeZ_MoeB
SeqID_120491
MoeZ/MoeB domain

Chitin_synth_2
SeqID_120016
Chitin synthase

PAP_central
SeqID_12343 SeqID_119183
Poly(A) polymerase central domain

SeqID_120181 SeqID_120989

SeqID_121025 SeqID_123810

rve
SeqID_119251 SeqID_119810
Integrase core domain

RED_N
SeqID_120411
RED-like protein N-terminal region

Ank
SeqID_123070 SeqID_119179
Ankyrin repeat

SeqID_119568 SeqID_119583

SeqID_119584 SeqID_119680

SeqID_119792 SeqID_119874

SeqID_120226 SeqID_121617

CKS
SeqID_122543 SeqID_121066
Cyclin-dependent kinase

regulatory subunit

Band_7
SeqID_122693 SeqID_119534
SPFH domain/Band 7 family

SeqID_123829

PAF-AH_p_II
SeqID_120737 SeqID_121196
Platelet-activating factor

acetylhydrolas

SF-assemblin
SeqID_120078
SF-assemblin/beta giardin

Ribosomal_S24e
SeqID_122807 SeqID_120291
Ribosomal protein S24e

SeqID_124204 SeqID_124210

SeqID_121954

Ribosomal_S17e
SeqID_122394 SeqID_119329
Ribosomal S17

SeqID_123964

Sof1
SeqID_122192 SeqID_120865
Sof1-like domain

SeqID_120866 SeqID_124334

LrgB
SeqID_120612
LrgB-like family

DUF1650
SeqID_122945 SeqID_123077
Protein of unknown function

SeqID_120203
(DUF1650)

Laminin_EGF
SeqID_121392 SeqID_119297
Laminin EGF-like (Domains III and

SeqID_119335 SeqID_119714
V)

SeqID_119816 SeqID_120849

SeqID_120911

TruB_N
SeqID_119847
TruB family pseudouridylate

synthase (N term

tRNA-synt_2b
SeqID_122133 SeqID_122759
tRNA synthetase class II core

SeqID_119832 SeqID_120346
domain (G,

SeqID_120388 SeqID_120642

SeqID_121608 SeqID_121085

SeqID_121645 SeqID_124158

SeqID_124247

tRNA-synt_2c
SeqID_120391
tRNA synthetases class II (A)

Innexin
SeqID_123073 SeqID_123480
Innexin

SeqID_120601

tRNA-synt_2d
SeqID_122320 SeqID_119409
tRNA synthetases class II core

SeqID_121659 SeqID_124019
domain (F

MFS_1
SeqID_119430 SeqID_119607
Major Facilitator Superfamily

SeqID_120612

Cyt-b5
SeqID_122136 SeqID_122505
Cytochrome b5-like Heme/Steroid

SeqID_119879 SeqID_123602
bindin

MAM33
SeqID_122105 SeqID_123650
Mitochondrial glycoprotein

ZZ
SeqID_120217
Zinc finger, ZZ type

Dpy-30
SeqID_122847 SeqID_121204
Dpy-30 motif

K_tetra
SeqID_122369 SeqID_119930
K+ channel tetramerisation domain

SeqID_120530

Tim44
SeqID_123264 SeqID_119402
Tim44-like domain

Mtap_PNP
SeqID_121534 SeqID_121548
Phosphorylase family 2

SeqID_121569

PDZ
SeqID_119401 SeqID_120527
PDZ domain (Also known as DHR

or GLGF)

CHCH
SeqID_123527
CHCH domain

Ribonuc_red_sm
SeqID_122881 SeqID_120548
Ribonucleotide reductase, small

SeqID_121571 SeqID_121595
chain

Pro_isomerase
SeqID_12368 SeqID_122436
Cyclophilin type peptidyl-prolyl cis-

SeqID_12839 SeqID_123273
tr

SeqID_121384 SeqID_119354

SeqID_120150 SeqID_123750

SeqID_123891 SeqID_121707

SeqID_124224

DIX
SeqID_123325
DIX domain

Hydrolase
SeqID_119435
haloacid dehalogenase-like

hydrolase

Peptidase_C1
SeqID_121248 SeqID_121247
Papain family cysteine protease

SeqID_121267 SeqID_122029

SeqID_122165 SeqID_122555

SeqID_122593 SeqID_123403

SeqID_123446 SeqID_121447

SeqID_119313 SeqID_123567

SeqID_123581 SeqID_123578

SeqID_121782 SeqID_121853

SeqID_121879

Peptidase_C2
SeqID_123259 SeqID_120192
Calpain family cysteine protease

SeqID_120374

E1-E2_ATPase
SeqID_122184 SeqID_119574
E1-E2 ATPase

SeqID_119844 SeqID_120162

Peptidase_M13
SeqID_122218
Peptidase family M13

FLYWCH
SeqID_119876 SeqID_120405
FLYWCH zinc finger domain

Peptidase_M14
SeqID_122059 SeqID_122194
Zinc carboxypeptidase

SeqID_119546 SeqID_123807

SeqID_124007

Sec62
SeqID_121454
Translocation protein Sec62

Sec63
SeqID_120914
Sec63 domain

Peptidase_M16
SeqID_123296 SeqID_119755
Insulinase (Peptidase family M16)

SeqID_124192

EGF
SeqID_119714 SeqID_119985
EGF-like domain

SeqID_120013 SeqID_120488

SeqID_120732 SeqID_120849

Ribonuc_red_IgC
SeqID_121385
Ribonucleotide reductase, barrel

doma

UPF0027
SeqID_121387
Uncharacterized protein family

UPF0027

APC10
SeqID_122598 SeqID_119693
Anaphase-promoting complex,

SeqID_123584
subunit 10 (APC1

Integrin_alpha
SeqID_119890
Integrin alpha cytoplasmic region

Dynein_heavy
SeqID_120763 SeqID_120973
Dynein heavy chain

Chromo
SeqID_119766 SeqID_120561
‘chromo’ (CHRromatin

Organisation MOdifier)

Surp
SeqID_122195 SeqID_120133
Surp module

SeqID_124100 SeqID_124099

Lipase_GDSL
SeqID_122975 SeqID_120739
GDSL-like Lipase/Acylhydrolase

ASC
SeqID_119784
Amiloride-sensitive sodium

channel

F-actin_cap_A
SeqID_123125 SeqID_119252
F-actin capping protein alpha

subunit

Ribosomal_L2_C
SeqID_122387 SeqID_122576
Ribosomal Proteins L2, C-terminal

SeqID_119709 SeqID_123662
doma

SeqID_124015 SeqID_121759

SeqID_121891

TPR_1
SeqID_122178 SeqID_122894
Tetratricopeptide repeat

SeqID_123022 SeqID_119202

SeqID_119274 SeqID_119429

SeqID_119528 SeqID_119687

SeqID_120074 SeqID_120605

SeqID_121168 SeqID_121175

SeqID_123940

NTP_transf_2
SeqID_122343 SeqID_123810
Nucleotidyltransferase domain

TPR_2
SeqID_122178 SeqID_122894
Tetratricopeptide repeat

SeqID_123022 SeqID_119202

SeqID_119274 SeqID_119429

SeqID_119528 SeqID_119687

SeqID_120074 SeqID_120591

SeqID_120605 SeqID_120748

SeqID_120947 SeqID_121168

SeqID_121175 SeqID_123940

TPR_4
SeqID_119202
Tetratricopeptide repeat

COesterase
SeqID_122237 SeqID_119940
Carboxylesterase

SeqID_120067 SeqID_120891

SeqID_124012

TLE_N
SeqID_123010 SeqID_119913
Groucho/TLE N-terminal Q-rich

domain

F-box
SeqID_122666 SeqID_124313
F-box domain

MRP-L47
SeqID_122709 SeqID_120115
Mitochondrial 39-S ribosomal

SeqID_124286
protein L47 (MR

Col_cuticle_N
SeqID_121964 SeqID_121988
Nematode cuticle collagen N-

SeqID_121996 SeqID_122013
terminal do

SeqID_122019 SeqID_122027

SeqID_122291 SeqID_122304

SeqID_122313 SeqID_122472

SeqID_122536 SeqID_122871

SeqID_122911 SeqID_122927

SeqID_122954 SeqID_123029

SeqID_123087 SeqID_123176

SeqID_123223 SeqID_123423

SeqID_119404 SeqID_119564

SeqID_119730 SeqID_119798

SeqID_120106 SeqID_120175

SeqID_120474 SeqID_120705

SeqID_120750 SeqID_123663

SeqID_121026 SeqID_121073

SeqID_123664 SeqID_123677

SeqID_123676 SeqID_123769

SeqID_123783 SeqID_123827

SeqID_121953 SeqID_121957

Na_H_Exchanger
SeqID_120225 SeqID_120403
Sodium/hydrogen exchanger

family

ATP-synt_ab
SeqID_122131 SeqID_122660
ATP synthase alpha/beta family,

SeqID_122734 SeqID_122935
nucleot

SeqID_119160 SeqID_123656

SeqID_123806 SeqID_123805

SeqID_124022

zf-B_box
SeqID_120931
B-box zinc finger

FMO-like
SeqID_122732 SeqID_120410
Flavin-binding monooxygenase-

SeqID_124057
like

Ribosomal_S26e
SeqID_122109 SeqID_120185
Ribosomal protein S26e

SeqID_123649

Ribosomal_S19e
SeqID_122794 SeqID_123065
Ribosomal protein S19e

SeqID_123504 SeqID_124003

SeqID_124229 SeqID_121859

Peptidase_C12
SeqID_122328 SeqID_120007
Ubiquitin carboxyl-terminal

SeqID_121135 SeqID_124005
hydrolase,

Peptidase_C13
SeqID_122230 SeqID_121453
Peptidase C13 family

SeqID_121467 SeqID_120219

SeqID_120537 SeqID_121597

SeqID_124041 SeqID_121721

SeqID_121872 SeqID_121883

Peptidase_C14
SeqID_123489
Caspase domain

Peptidase_M24
SeqID_122645 SeqID_124035
metallopeptidase family M24

SeqID_124033

Ribosomal_L6e_N
SeqID_122489 SeqID_123683
Ribosomal protein L6, N-terminal

doma

Paf1
SeqID_119976
Paf1

DUF1671
SeqID_123049
Protein of unknown function

(DUF1671)

Complex1_51K
SeqID_122912 SeqID_123104
Respiratory-chain NADH

SeqID_123995
dehydrogenase 51

Peptidase_M28
SeqID_122327 SeqID_122621
Peptidase family M28

SeqID_119445 SeqID_123920

PFK
SeqID_120213
Phosphofructokinase

DAGAT
SeqID_123153 SeqID_120048
Diacylglycerol acyltransferase

SeqID_121202

RNA_pol_Rpb7_N
SeqID_122067 SeqID_121484
RNA polymerase Rpb7, N-terminal

SeqID_121111 SeqID_123690
domain

DnaJ_C
SeqID_121279 SeqID_122722
DnaJ C terminal region

SeqID_121405 SeqID_120879

SeqID_123882

zf-DNL
SeqID_122584 SeqID_124310
DNL zinc finger

CNH
SeqID_123483 SeqID_120283
CNH domain

SeqID_121627

DNA_ligase_A_M
SeqID_123044 SeqID_119293
ATP dependent DNA ligase

domain

DNA_ligase_A_N
SeqID_119293
DNA ligase N terminus

LACT
SeqID_119637
Lecithin:cholesterol

acyltransferase

Ribosomal_L11_N
SeqID_122466 SeqID_122612
Ribosomal protein L11, N-terminal

SeqID_122798 SeqID_120200
dom

SeqID_120321 SeqID_121603

SeqID_123787 SeqID_124218

Sec8_exocyst
SeqID_123021
Sec8 exocyst complex component

specific

Coatomer_E
SeqID_122494 SeqID_120679
Coatomer epsilon subunit

SeqID_123777

TT_ORF2
SeqID_122828 SeqID_123933
TT viral ORF2

DNA_primase_S
SeqID_122299 SeqID_123968
DNA primase small subunit

NACHT
SeqID_122493 SeqID_119176
NACHT domain

Ribosomal_S27e
SeqID_122380 SeqID_122513
Ribosomal protein S27

SeqID_119700 SeqID_120329

SeqID_123636 SeqID_123738

Na_K-ATPase
SeqID_122309 SeqID_124043
Sodium/potassium ATPase beta

chain

TIP49
SeqID_122138 SeqID_121376
TIP49 C-terminus

SeqID_119157 SeqID_119793

SeqID_123801

GNT-I
SeqID_119837
GNT-I family

Clathrin
SeqID_119727
Region in Clathrin and VPS

MutS_V
SeqID_119581 SeqID_119594
MutS domain V

SeqID_120288 SeqID_120369

Acyl_CoA_thio
SeqID_122480 SeqID_121327
Acyl-CoA thioesterase

SeqID_123795 SeqID_121650

SeqID_121919

PTPA
SeqID_121340 SeqID_123363
Phosphotyrosyl phosphate

SeqID_119880
activator (PTPA) pr

UPF0113
SeqID_122707
Uncharacterised protein family

(UPF0113)

dsrm
SeqID_119344
Double-stranded RNA binding

motif

Tom22
SeqID_122077 SeqID_124129
Mitochondrial import receptor

subunit Tom22

EGF_CA
SeqID_119714 SeqID_119985
Calcium binding EGF domain

SeqID_120013 SeqID_120732

lsy1
SeqID_122830 SeqID_120949
lsy1-like splicing family

ELM2
SeqID_120201 SeqID_120354
ELM2 domain

SeqID_121080

HA2
SeqID_122331 SeqID_119298
Helicase associated domain (HA2)

SeqID_120256 SeqID_120575

SeqID_123546 SeqID_124253

RdRP
SeqID_119588
RNA dependent RNA polymerase

2-oxoacid_dh
SeqID_119602 SeqID_121525
2-oxoacid dehydrogenases

SeqID_120978
acyltransferase

UDPGP
SeqID_123086
UTP--glucose-1-phosphate

uridylyltransferase

Arf
SeqID_121288 SeqID_122204
ADP-ribosylation factor family

SeqID_122360 SeqID_122392

SeqID_122397 SeqID_122560

SeqID_122575 SeqID_122579

SeqID_122619 SeqID_122671

SeqID_123132 SeqID_123241

SeqID_123422 SeqID_119204

SeqID_119645 SeqID_119853

SeqID_120523 SeqID_120531

SeqID_121613 SeqID_123568

SeqID_123726 SeqID_123740

SeqID_123960 SeqID_123988

SeqID_123991

UDPGT
SeqID_119517
UDP-glucoronosyl and UDP-

glucosyl transferas

Cystatin
SeqID_122804 SeqID_120505
Cystatin domain

SeqID_123974

ATP-synt_F6
SeqID_122448 SeqID_120742
Mitochondrial ATP synthase

SeqID_123680
coupling facto

Cyto_heme_lyase
SeqID_122106 SeqID_124078
Cytochrome c/c1 heme lyase

DNA_pol_alpha_B
SeqID_120691
DNA polymerase alpha subunit B

Arm
SeqID_122305 SeqID_122870
Armadillo/beta-catenin-like repeat

SeqID_121423 SeqID_119427

SeqID_121148 SeqID_121201

SeqID_124047 SeqID_121836

NTP_transferase
SeqID_122569 SeqID_122914
Nucleotidyl transferase

SeqID_120001

LSM
SeqID_122052 SeqID_122121
LSM domain

SeqID_122135 SeqID_122405

SeqID_122422 SeqID_122654

SeqID_122720 SeqID_122860

SeqID_123047 SeqID_119181

SeqID_119424 SeqID_120049

SeqID_120122 SeqID_121030

SeqID_123658 SeqID_123725

SeqID_123789 SeqID_123944

SeqID_124195

NMT
SeqID_120382
Myristoyl-CoA:protein N-

myristoyltransferase

TRAP-delta
SeqID_122068 SeqID_120359
Translocon-associated protein,

SeqID_123786
delta subun

DDOST_48kD
SeqID_121541 SeqID_121553
Dolichyl-

diphosphooligosaccharide-protein

Ribosomal_S28e
SeqID_122545 SeqID_120643
Ribosomal protein S28e

SeqID_123620

UPF0120
SeqID_122142 SeqID_121137
Uncharacterised protein family

(UPF0120)

Ala_racemase_N
SeqID_121220
Alanine racemase, N-terminal

domain

MFAP1_C
SeqID_119946
Micro-fibrillar-associated protein 1

C-termi

Aminotran_3
SeqID_119827
Aminotransferase class-III

ACBP
SeqID_121287 SeqID_119464
Acyl CoA binding protein

PHD
SeqID_123154 SeqID_119379
PHD-finger

SeqID_119449 SeqID_119766

SeqID_120788

Aminotran_4
SeqID_122578 SeqID_119653
Aminotransferase class IV

SeqID_119738

E3_binding
SeqID_121975 SeqID_120333
e3 binding domain

Ribosomal_L37ae
SeqID_122763 SeqID_119302
Ribosomal L37ae protein family

SeqID_120838 SeqID_123781

SeqID_124249

zf-CCCH
SeqID_121226 SeqID_122128
Zinc finger C-x8-C-x5-C-x3-H type

SeqID_122531 SeqID_122888
(and simil

SeqID_123330 SeqID_119331

SeqID_119820 SeqID_120375

SeqID_120658 SeqID_121599

SeqID_121064 SeqID_123669

HMG-CoA_red
SeqID_120545
Hydroxymethylglutaryl-coenzyme

A reductus

CRAL_TRIO
SeqID_120518
CRAL/TRIO domain

HAT
SeqID_120676
HAT (Half-A-TPR) repeat

Asn_synthase
SeqID_120554 SeqID_120715
Asparagine synthase

PDCD9
SeqID_122558 SeqID_123575
Mitochondrial 28S ribosomal

protein S30 (PDC

zf-CSL
SeqID_122103 SeqID_120511
CSL zinc finger

SeqID_123712

UBACT
SeqID_122346 SeqID_121362
Repeat in ubiquitin-activating

SeqID_121432 SeqID_121452
(UBA) protein

SeqID_119364 SeqID_124102

SeqID_121935

Biotin_carb_C
SeqID_120976
Biotin carboxylase C-terminal

domain

Frizzled
SeqID_120827
Frizzled/Smoothened family

membrane region

FH2
SeqID_120125
Formin Homology 2 Domain

Coatomer_WDAD
SeqID_119681 SeqID_120257
Coatomer WD associated region

zf-RanBP
SeqID_123008 SeqID_121015
Zn-finger in Ran binding protein

and oth

COQ7
SeqID_122535 SeqID_119203
Ubiquinone biosynthesis protein

SeqID_123627
COQ7

Ammonium_transp
SeqID_120017
Ammonium Transporter Family

PTR2
SeqID_119430 SeqID_120270
POT family

MPPN
SeqID_119150
MPPN (rrm-like) domain

Pyr_redox
SeqID_119535 SeqID_120156
Pyridine nucleotide-disulphide

oxidoreducta

Carn_acyltransf
SeqID_119995
Choline/Carnitine o-

acyltransferase

TUDOR
SeqID_121121
Tudor domain

Krr1
SeqID_120316
Krr1 family

Asp
SeqID_122475 SeqID_123036
Eukaryotic aspartyl protease

SeqID_123118 SeqID_123359

SeqID_121455 SeqID_120476

SeqID_120494 SeqID_123971

BACK
SeqID_120833 SeqID_121637
BTB And C-terminal Kelch

Uso1_p115_head
SeqID_122989
Uso1/p115 like vesicle tethering

pro

ATP-synt_ab_C
SeqID_122371 SeqID_122660
ATP synthase alpha/beta chain, C

SeqID_123656 SeqID_123806
termin

SeqID_123805

Thiolase_C
SeqID_122429 SeqID_122772
Thiolase, C-terminal domain

SeqID_120310 SeqID_123864

5_nucleotid
SeqID_120648
5′ nucleotidase family

FHA
SeqID_120176 SeqID_120836
FHA domain

PID
SeqID_123247
Phosphotyrosine interaction

domain (PTB/PID)

Citrate_synt
SeqID_122637 SeqID_122705
Citrate synthase

SeqID_120172 SeqID_124243

Helicase_C
SeqID_122054 SeqID_122318
Helicase conserved C-terminal

SeqID_122331 SeqID_122336
domain

SeqID_122624 SeqID_122677

SeqID_119233 SeqID_119298

SeqID_119324 SeqID_119620

SeqID_119843 SeqID_119917

SeqID_120153 SeqID_120168

SeqID_120256 SeqID_120323

SeqID_120342 SeqID_120575

SeqID_120689 SeqID_120814

SeqID_120914 SeqID_121032

SeqID_123546 SeqID_121674

SeqID_124119 SeqID_124137

SeqID_124148 SeqID_124325

Neur_chan_LBD
SeqID_121005 SeqID_121087
Neurotransmitter-gated ion-

channel lig

Ion_trans_2
SeqID_121451 SeqID_119613
Ion channel

SeqID_119930 SeqID_119939

Myosin_tail_1
SeqID_123386
Myosin tail

TMS_TDE
SeqID_122691 SeqID_119438
TMS membrane protein/tumour

SeqID_124263
differentially e

P16-Arc
SeqID_119921
ARP2/3 complex 16 kDa subunit

(p16-Arc)

ATP-synt_ab_N
SeqID_122110 SeqID_122131
ATP synthase alpha/beta family,

SeqID_122660 SeqID_119160
beta-ba

SeqID_123806 SeqID_123811

SeqID_124022

Endonuclease_NS
SeqID_123055
DNA/RNA non-specific

endonuclease

Thiolase_N
SeqID_122429 SeqID_122772
Thiolase, N-terminal domain

SeqID_121350 SeqID_122890

SeqID_122953 SeqID_123288

SeqID_123365 SeqID_121440

SeqID_121529 SeqID_120310

SeqID_123593 SeqID_123864

Glycogen_syn
SeqID_121477 SeqID_120492
Glycogen synthase

BTB
SeqID_119616 SeqID_120373
BTB/POZ domain

SeqID_121165 SeqID_121637

DUF236
SeqID_119224 SeqID_119247
Protein of unknown function

SeqID_120712 SeqID_120713

Ubiq_cyt_C_chap
SeqID_122228 SeqID_121449
Ubiquinol-cytochrome C

SeqID_119888 SeqID_123755
chaperone

Renin_r
SeqID_122129 SeqID_121442
Renin receptor-like protein

SeqID_123862

Cwf_Cwc_15
SeqID_122116 SeqID_120731
Cwf15/Cwc15 cell cycle control

SeqID_121658 SeqID_124014
protein

A_deaminase
SeqID_121003
Adenosine/AMP deaminase

DUF164
SeqID_121260 SeqID_123386
Uncharacterized ACR, COG1579

ATP-synt_C
SeqID_122557 SeqID_122592
ATP synthase subunit C

SeqID_122805 SeqID_123522

SeqID_119902 SeqID_119958

SeqID_119959 SeqID_120137

SeqID_124207 SeqID_124336

ATP-synt_D
SeqID_122796 SeqID_123297
ATP synthase subunit D

SeqID_123366 SeqID_123854

LAG1
SeqID_121272 SeqID_122595
Longevity-assurance protein

SeqID_121367
(LAG1)

ATP-synt_E
SeqID_123534
ATP synthase E chain

ATP-synt_F
SeqID_122088 SeqID_121348
ATP synthase (F/14-kDa) subunit

SeqID_121745 SeqID_121827

SeqID_121881

Filamin
SeqID_120307 SeqID_120510
Filamin/ABP280 repeat

MazG
SeqID_119952
MazG nucleotide

pyrophosphohydrolase domain

ATP-synt_G
SeqID_122669 SeqID_119783
Mitochondrial ATP synthase g

SeqID_124110
subunit

EF1_GNE
SeqID_121982 SeqID_122152
EF-1 guanine nucleotide exchange

SeqID_122572 SeqID_119628
domain

SeqID_119899 SeqID_123606

FAD_binding_1
SeqID_122094 SeqID_122444
FAD binding domain

SeqID_123823

FAD_binding_2
SeqID_120807
FAD binding domain

Dynein_light
SeqID_122635 SeqID_123252
Dynein light chain type 1

SeqID_119812 SeqID_123564

FAD_binding_3
SeqID_122411 SeqID_123933
FAD binding domain

Astacin
SeqID_122226 SeqID_122581
Astacin (Peptidase family M12A)

SeqID_123481 SeqID_119601

SeqID_120015 SeqID_120571

SeqID_120933 SeqID_121174

SeqID_121216 SeqID_124300

Tfb4
SeqID_122561 SeqID_123565
Transcription factor Tfb4

Adaptin_N
SeqID_120538 SeqID_120950
Adaptin N terminal region

EFG_IV
SeqID_121397 SeqID_120540
Elongation factor G, domain IV

S-AdoMet_synt_C
SeqID_123143 SeqID_119596
S-adenosylmethionine synthetase,

C-te

Ribosomal_S10
SeqID_122673 SeqID_119858
Ribosomal protein S10p/S20e

SeqID_123703 SeqID_121948

Ribosomal_S11
SeqID_122146 SeqID_123024
Ribosomal protein S11

SeqID_123430 SeqID_120073

SeqID_120744 SeqID_123697

SeqID_124177

Ribosomal_S12
SeqID_122060 SeqID_123235
Ribosomal protein S12

SeqID_123361 SeqID_123428

SeqID_123434 SeqID_123629

SeqID_124279

UCR_hinge
SeqID_122302 SeqID_123516
Ubiquinol-cytochrome C reductase

SH3_1
SeqID_123228 SeqID_119323
SH3 domain

SeqID_120958

Ribosomal_S13
SeqID_122782 SeqID_122809
Ribosomal protein S13/S18

SeqID_123978 SeqID_124200

SAP
SeqID_120587
SAP domain

SH3_2
SeqID_123228 SeqID_120958
Variant SH3 domain

Ribosomal_S14
SeqID_122064 SeqID_122107
Ribosomal protein S14p/S29e

SeqID_120012 SeqID_121048

SeqID_121107 SeqID_123630

SeqID_123890

Brix
SeqID_121565 SeqID_123250
Brix domain

SeqID_119314 SeqID_120464

SeqID_120490 SeqID_121589

SeqID_121109

Ribosomal_S15
SeqID_122373 SeqID_123410
Ribosomal protein S15

SeqID_123433 SeqID_121412

SeqID_123718

Ras
SeqID_121288 SeqID_122204
Ras family

SeqID_122360 SeqID_122392

SeqID_122397 SeqID_122560

SeqID_122575 SeqID_122579

SeqID_122619 SeqID_122671

SeqID_122838 SeqID_122872

SeqID_122903 SeqID_123052

SeqID_123189 SeqID_123241

SeqID_123414 SeqID_123422

SeqID_119204 SeqID_119290

SeqID_119645 SeqID_119708

SeqID_119885 SeqID_119891

SeqID_120379 SeqID_120664

SeqID_121613 SeqID_120982

SeqID_123605 SeqID_121093

SeqID_123568 SeqID_123726

SeqID_123740 SeqID_121691

SeqID_123960 SeqID_123988

SeqID_123991 SeqID_121918

zf-TAZ
SeqID_120357
TAZ zinc finger

Ribosomal_S17
SeqID_122602 SeqID_122629
Ribosomal protein S17

SeqID_119883 SeqID_123918

SeqID_123925

Ribosomal_S18
SeqID_122652
Ribosomal protein S18

ELK
SeqID_120763
ELK domain

Ribosomal_S19
SeqID_122056 SeqID_123333
Ribosomal protein S19

SeqID_123450 SeqID_123685

C2
SeqID_119599 SeqID_119684
C2 domain

SeqID_120248

eIF-5_eIF-2B
SeqID_123321 SeqID_119265
Domain found in IF2B/IF5

SeqID_120603 SeqID_121769

VWD
SeqID_122463 SeqID_123112
von Willebrand factor type D

SeqID_119726 SeqID_123592
domain

GHMP_kinases
SeqID_123199
GHMP kinases putative ATP-

PAP_RNA-bind
SeqID_120989
Poly(A) polymerase predicted RNA

binding

C4
SeqID_119273 SeqID_119634
C-terminal tandem repeated

domain in t

P_proprotein
SeqID_121230 SeqID_122663
Proprotein convertase P-domain

SeqID_123014 SeqID_123393

ELO
SeqID_122222 SeqID_122315
GNS1/SUR4 family

SeqID_119651 SeqID_121493

SeqID_120991 SeqID_124009

SeqID_124028

p450
SeqID_120075 SeqID_120579
Cytochrome P450

Complex1_30kDa
SeqID_121297 SeqID_119579
Respiratory-chain NADH

dehydrogenase,

wnt
SeqID_122540
wnt family

OATP
SeqID_119717
Organic Anion Transporter

Polypeptide (OATP)

RIIa
SeqID_122847 SeqID_121204
Regulatory subunit of type II PKA

R-subunit

Pyrophosphatase
SeqID_122209 SeqID_123850
Inorganic pyrophosphatase

A1_Propeptide
SeqID_122475 SeqID_123036
A1 Propeptide

SeqID_123359 SeqID_121455

SeqID_120476 SeqID_120494

SeqID_123971

PRP1_N
SeqID_121212
PRP1 splicing factor, N-terminal

RhoGAP
SeqID_120222 SeqID_120311
RhoGAP domain

G-alpha
SeqID_120144 SeqID_120531
G-protein alpha subunit

SeqID_120703 SeqID_121138

Guanylate_kin
SeqID_120766
Guanylate kinase

HSF_DNA-bind
SeqID_123050
HSF-type DNA-binding

DnaJ_CXXCXGXG
SeqID_121279 SeqID_122722
DnaJ central domain (4 repeats)

SeqID_121405 SeqID_121507

SeqID_120879 SeqID_121218

SeqID_123882

Collagen
SeqID_121964 SeqID_121996
Collagen triple helix repeat (20

SeqID_122013 SeqID_122019
copies)

SeqID_122027 SeqID_122216

SeqID_122291 SeqID_122304

SeqID_122313 SeqID_122472

SeqID_122536 SeqID_122650

SeqID_121377 SeqID_122871

SeqID_122911 SeqID_122954

SeqID_123029 SeqID_123087

SeqID_123176 SeqID_123223

SeqID_123423 SeqID_119253

SeqID_119404 SeqID_119418

SeqID_119540 SeqID_119564

SeqID_119634 SeqID_119798

SeqID_119948 SeqID_119971

SeqID_120106 SeqID_120175

SeqID_120214 SeqID_120361

SeqID_120474 SeqID_120626

SeqID_120674 SeqID_120705

SeqID_123663 SeqID_121026

SeqID_121073 SeqID_121150

SeqID_123664 SeqID_123677

SeqID_123676 SeqID_123769

SeqID_123783 SeqID_123827

SeqID_121953 SeqID_121958

Tubulin_C
SeqID_122269 SeqID_122332
Tubulin/FtsZ family, C-terminal

SeqID_122440 SeqID_119159
domain

SeqID_119381 SeqID_119720

SeqID_120775 SeqID_123545

SeqID_123884 SeqID_123998

SeqID_121849 SeqID_121940

JmjC
SeqID_122284 SeqID_120520
jmjC domain

Zona_pellucida
SeqID_119201 SeqID_120047
Zona pellucida-like domain

CH
SeqID_122025 SeqID_122264
Calponin homology (CH) domain

SeqID_122337 SeqID_122473

SeqID_122788 SeqID_123499

SeqID_119750 SeqID_120539

SeqID_123855 SeqID_124085

SeqID_124206

E-MAP-115
SeqID_120063
E-MAP-115 family

CPSase_L_D2
SeqID_119260 SeqID_119619
Carbamoyl-phosphate synthase L

SeqID_120976
chain, A

HMG_box
SeqID_122876 SeqID_122918
HMG (high mobility group) box

SeqID_121497 SeqID_120582

SeqID_120832 SeqID_120943

Ribosomal_S25
SeqID_122611 SeqID_122695
S25 ribosomal protein

SeqID_123417 SeqID_123597

SeqID_124222 SeqID_124340

Ribosomal_S27
SeqID_122053 SeqID_122726
Ribosomal protein S27a

SeqID_121490 SeqID_123637

SeqID_123659

Galactosyl_T
SeqID_120408
Galactosyltransferase

CS
SeqID_122778 SeqID_122828
CS domain

SeqID_123335 SeqID_121480

SeqID_120610

Voltage_CLC
SeqID_120735 SeqID_121213
Voltage gated chloride channel

Lactamase_B
SeqID_120930
Metallo-beta-lactamase

superfamily

eRF1_1
SeqID_119244
eRF1 domain 1

eRF1_2
SeqID_123076 SeqID_119244
eRF1 domain 2

Flavoprotein
SeqID_122326 SeqID_119773
Flavoprotein

eRF1_3
SeqID_123076 SeqID_119244
eRF1 domain 3

EMP24_GP25L
SeqID_122517 SeqID_119571
emp24/gp25L/p24 family

SeqID_123733

NOT2_3_5
SeqID_122445 SeqID_121346
NOT2/NOT3/NOT5 family

SeqID_123876

Hydantoinase_A
SeqID_121246 SeqID_121266
Hydantoinase/oxoprolinase

Plus-3
SeqID_122270
Plus-3 domain

IBB
SeqID_122870 SeqID_121423
Importin beta binding domain

SeqID_119952 SeqID_121148

Ald_Xan_dh_C2
SeqID_120485 SeqID_121912
Aldehyde oxidase and xanthine

dehydroge

Complex1_49kDa
SeqID_120479
Respiratory-chain NADH

dehydrogenase,

SSrecog
SeqID_120076
Structure-specific recognition

protein

Aldo_ket_red
SeqID_123277
Aldo/keto reductase family

TFIIS_C
SeqID_122597 SeqID_123714
Transcription factor S-II (TFIIS)

Thioredoxin
SeqID_121221 SeqID_122115
Thioredoxin

SeqID_122712 SeqID_123314

SeqID_120315 SeqID_120528

SeqID_121134 SeqID_123686

SeqID_123796 SeqID_121887

vATP-synt_E
SeqID_122754 SeqID_121463
ATP synthase (E/31 kDa) subunit

SeqID_119218 SeqID_124020

SeqID_121724

Metallothio
SeqID_119714
Metallothinein

Mo-co_dimer
SeqID_122426 SeqID_119301
Mo-co oxidoreductase dimensation

SeqID_123908
doma

ORC2
SeqID_121926
Origin recognition complex subunit 2

Ribosomal_S30
SeqID_122415 SeqID_123507
Ribosomal protein S30

SeqID_120390 SeqID_123707

SCP
SeqID_121229 SeqID_121227
SCP-like extracellular protein

SeqID_121228 SeqID_122770

SeqID_122767 SeqID_122864

SeqID_123501 SeqID_121425

SeqID_119399 SeqID_119556

SeqID_120347 SeqID_121641

GPP34
SeqID_120614
Golgi phosphoprotein 3 (GPP34)

DM
SeqID_121139
DM DNA binding domain

GTP_CDC
SeqID_119791
Cell division protein

AhpC-TSA
SeqID_122512 SeqID_122760
AhpC/TSA family

SeqID_123258 SeqID_123300

SeqID_119348 SeqID_123739

SeqID_123758

CAP_GLY
SeqID_123227
CAP-Gly domain

XRCC1_N
SeqID_120679
XRCC1 N terminal domain

DUF26
SeqID_122879
Domain of unknown function

DUF26

TRAP_beta
SeqID_122465 SeqID_119980
Translocon-associated protein

SeqID_123848
beta (TRAPB)

Cation_ATPase_N
SeqID_122184 SeqID_119574
Cation transporter/ATPase, N-

SeqID_119844 SeqID_120162
terminus

SeqID_124141 SeqID_124234

XPG_I
SeqID_120903
XPG I-region

TFIID-31kDa
SeqID_122432 SeqID_123899
Transcription initiation factor IID,

31 kD

ARPC4
SeqID_123032 SeqID_121098
ARP2/3 complex 20 kDa subunit

(ARPC4)

Nop
SeqID_122208 SeqID_119511
Putative snoRNA binding domain

SeqID_120274 SeqID_123696

DX
SeqID_119623
DX module

Choline_kinase
SeqID_122682 SeqID_124140
Choline/ethanolamine kinase

SeqID_121776

Seryl_tRNA_N
SeqID_122169 SeqID_124027
Seryl-tRNA synthetase N-terminal

domain

UFD1
SeqID_122278 SeqID_122991
Ubiquitin fusion degradation

SeqID_120363 SeqID_123652
protein UFD1

AICARFT_IMPCHas
SeqID_121071
AICARFT/IMPCHase bienzyme

Adap_comp_sub
SeqID_122747 SeqID_119495
Adaptor complexes medium

SeqID_120295
subunit family

V_ATPase_I
SeqID_121161
V-type ATPase 116 kDa subunit

family

eIF-6
SeqID_122640 SeqID_120843
eIF-6 family

SeqID_123945

TAF
SeqID_121234 SeqID_122483
TATA box binding protein

SeqID_122744 SeqID_122771
associated fac

SeqID_119330 SeqID_121479

SeqID_121482 SeqID_124258

SeqID_124262

HSP70
SeqID_121224 SeqID_121246
Hsp70 protein

SeqID_121258 SeqID_121266

SeqID_122340 SeqID_122574

SeqID_121338 SeqID_123128

SeqID_123276 SeqID_121398

SeqID_121394 SeqID_121403

SeqID_121456 SeqID_119310

SeqID_119878 SeqID_121465

SeqID_121528 SeqID_120193

SeqID_120276 SeqID_120425

SeqID_120426 SeqID_120477

SeqID_120618 SeqID_120675

SeqID_123580 SeqID_121647

SeqID_123958 SeqID_121785

Rho_GDI
SeqID_123503 SeqID_119733
RHO protein GDP dissociation

SeqID_121942
inhibitor

E2F_TDP
SeqID_122353 SeqID_120460
E2F/DP family winged-helix DNA-

SeqID_120823 SeqID_124183
binding domai

VPS28
SeqID_122699 SeqID_119677
VPS28 protein

SeqID_124233

SNARE
SeqID_122431 SeqID_123902
SNARE domain

CBM_14
SeqID_120683
Chitin binding Peritrophin-A

domain

Fic
SeqID_119770
Fic protein family

Peptidase_M16_C
SeqID_123083 SeqID_119755
Peptidase M16 inactive domain

efhand
SeqID_122031 SeqID_122171
EF hand

SeqID_122356 SeqID_122443

SeqID_122544 SeqID_122641

SeqID_122769 SeqID_122783

SeqID_122802 SeqID_122976

SeqID_123119 SeqID_119228

SeqID_119553 SeqID_119614

zf-CCHC
SeqID_122365 SeqID_122548
Zinc knuckle

SeqID_123105 SeqID_121406

SeqID_121418 SeqID_119748

SeqID_119884 SeqID_119889

SeqID_121017 SeqID_121081

SeqID_121192 SeqID_123993

Rieske
SeqID_119282
Rieske [2Fe—2S] domain

EF_TS
SeqID_122638 SeqID_121030
Elongation factor TS

GTP_EFTU
SeqID_122392 SeqID_122600
Elongation factor Tu GTP binding

SeqID_122657 SeqID_121329
domain

SeqID_122792 SeqID_122872

SeqID_123241 SeqID_121430

SeqID_119311 SeqID_119332

SeqID_119352 SeqID_119600

SeqID_120413 SeqID_120540

SeqID_121558 SeqID_121576

SeqID_121613 SeqID_123754

SeqID_121709 SeqID_123950

SeqID_124180 SeqID_121717

SeqID_121740 SeqID_121805

SeqID_121824

TPD52
SeqID_120171
Tumour protein D52 family

UCR_TM
SeqID_119282 SeqID_121586
Ubiquinol cytochrome reductase

SeqID_121732 SeqID_121833
transmembrane

Coq4
SeqID_120870
Coenzyme Q (ubiquinone)

biosynthesis protein

zf-HIT
SeqID_123532
HIT zinc finger

CUB
SeqID_123097 SeqID_119304
CUB domain

DUF32
SeqID_119459 SeqID_119801
Domain of unknown function

SeqID_121149
DUF32

adh_short
SeqID_121285 SeqID_122087
short chain dehydrogenase

SeqID_122187 SeqID_122421

SeqID_122523 SeqID_122530

SeqID_122596 SeqID_121476

SeqID_120081 SeqID_120916

SeqID_123628 SeqID_123706

SeqID_123772 SeqID_123943

RGS
SeqID_121152
Regulator of G protein signaling

PMM
SeqID_122570 SeqID_124322
Eukaryotic phosphomannomutase

ER
SeqID_121000
Enhancer of rudimentary

Patched
SeqID_120169 SeqID_120785
Patched family

SeqID_120998

RRM_1
SeqID_121236 SeqID_121239
RNA recognition motif. (a.k.a.

SeqID_122099 SeqID_122100
RRM, RBD, or

SeqID_122122 SeqID_122126

SeqID_122158 SeqID_122202

SeqID_122250 SeqID_122361

SeqID_122469 SeqID_122526

SeqID_122528 SeqID_122549

SeqID_122676 SeqID_122700

SeqID_122831 SeqID_122888

SeqID_122938 SeqID_122962

SeqID_123004 SeqID_123008

SeqID_123071 SeqID_123101

SeqID_123331 SeqID_123370

SeqID_123384 SeqID_123396

SeqID_123424 SeqID_119150

SeqID_119180 SeqID_119191

SeqID_119215 SeqID_119241

SeqID_124159 SeqID_119361

SeqID_119362 SeqID_119398

SeqID_119489 SeqID_119527

SeqID_119551 SeqID_124128

I-set
SeqID_119655 SeqID_119820
Immunoglobulin I-set domain

SeqID_119864 SeqID_119970

SeqID_121498 SeqID_121513

SeqID_120027 SeqID_123815

SeqID_120174 SeqID_120251

SeqID_120319 SeqID_120336

SeqID_120348 SeqID_120467

SeqID_120468 SeqID_120514

SeqID_120570 SeqID_120741

SeqID_120765 SeqID_123671

SeqID_121559 SeqID_121578

SeqID_121599 SeqID_121600

SeqID_121609 SeqID_120880

SeqID_120956 SeqID_121015

SeqID_121055 SeqID_121119

SeqID_121160 SeqID_123601

SeqID_123673 SeqID_123785

SeqID_123791 SeqID_123809

SeqID_123819 SeqID_123820

SeqID_123839 SeqID_121697

SeqID_124070 SeqID_124091

SeqID_124152 SeqID_124176

SeqID_124194 SeqID_124259

SeqID_121731 SeqID_121748

SeqID_121845 SeqID_121882

SeqID_121928 SeqID_121933

SeqID_123529 SeqID_119158

SeqID_119196 SeqID_119491

SeqID_119569 SeqID_119713

SeqID_119816 SeqID_120343

TBC
SeqID_119295 SeqID_119731
TBC domain

SeqID_121193

Calpain_III
SeqID_120374
Calpain large subunit, domain III

CBFD_NFYB_HMF
SeqID_121234 SeqID_122039
Histone-like transcription factor

SeqID_122046 SeqID_122119
(CBF/

SeqID_122455 SeqID_122483

SeqID_122582 SeqID_122688

SeqID_122744 SeqID_122766

SeqID_122771 SeqID_121314

SeqID_123003 SeqID_123317

SeqID_123463 SeqID_123473

SeqID_121448 SeqID_121446

SeqID_119330 SeqID_119493

SeqID_119512 SeqID_119521

SeqID_119609 SeqID_119776

SeqID_121479 SeqID_121482

SeqID_120790 SeqID_121601

SeqID_123744 SeqID_121643

SeqID_121663 SeqID_121675

SeqID_121684 SeqID_121688

SeqID_121706 SeqID_124105

SeqID_124164 SeqID_124258

SeqID_124262 SeqID_124276

SeqID_121771 SeqID_121809

SeqID_121823 SeqID_121905

UDPG_MGDP_dh_C
SeqID_123494
UDP-glucose/GDP-mannose

dehydrogenase

zf-UBP
SeqID_122511 SeqID_122662
Zn-finger in ubiquitin-hydrolases

SeqID_120244
and other

UPF0184
SeqID_122379 SeqID_123860
Uncharacterised protein family

(UPF0184)

FF
SeqID_121478 SeqID_120846
FF domain

SeqID_121180

Rhodanese
SeqID_123181 SeqID_123269
Rhodanese-like domain

TBP
SeqID_120108 SeqID_121146
Transcription factor TFIID (or

SeqID_121734
TATA-binding

Cytochrom_C1
SeqID_119212
Cytochrome C1 family

PI-PLC-Y
SeqID_120248
Phosphatidylinositol-specific

phospholipase

Glycolytic
SeqID_121242 SeqID_121268
Fructose-bisphosphate aldolase

SeqID_122585 SeqID_121503
class-I

SeqID_120227 SeqID_120758

SeqID_123730 SeqID_124335

SeqID_121856

COX15-CtaA
SeqID_119736
Cytochrome oxidase assembly

Actin
SeqID_121231 SeqID_121257
Actin

SeqID_122239 SeqID_122271

SeqID_122678 SeqID_122922

SeqID_122921 SeqID_122940

SeqID_122974 SeqID_122977

SeqID_122985 SeqID_123092

SeqID_123347 SeqID_123374

SeqID_123381 SeqID_123376

SeqID_123378 SeqID_123388

SeqID_123397 SeqID_123394

SeqID_123402 SeqID_119184

SeqID_121411 SeqID_119898

SeqID_121581 SeqID_121592

SeqID_123541 SeqID_123542

SeqID_123817 SeqID_123843

SeqID_121690 SeqID_124081

SeqID_124084 SeqID_124093

SeqID_124188 SeqID_124303

SeqID_121834 SeqID_121888

SeqID_121911

ATP_synt_H
SeqID_122810 SeqID_120261
ATP synthase subunit H

SeqID_124197

SET
SeqID_122866 SeqID_123437
SET domain

SeqID_119452 SeqID_119703

SeqID_121141

Ribosomal_L5_C
SeqID_121970 SeqID_122399
ribosomal L5P family C-terminus

SeqID_122665 SeqID_123116

SeqID_123183 SeqID_120376

SeqID_123547 SeqID_124178

ADK_lid
SeqID_121282 SeqID_122317
Adenylate kinase, active site lid

GrpE
SeqID_122071 SeqID_123157
GrpE

SeqID_123878 SeqID_121526

SeqID_120867 SeqID_123661

SeqID_121743

XRN_N
SeqID_122345 SeqID_121207
XRN 5′-3′ exonuclease N-terminus

SeqID_124106

Ribosomal_L1
SeqID_122679 SeqID_122715
Ribosomal protein L1p/L10e family

SeqID_124062

RhoGEF
SeqID_122254 SeqID_120745
RhoGEF domain

Y_phosphatase
SeqID_123060 SeqID_119220
Protein-tyrosine phosphatase

SeqID_119686 SeqID_119866

SeqID_119975 SeqID_120184

SeqID_120234 SeqID_120792

SeqID_121037 SeqID_121036

SeqID_121105

Ribosomal_L2
SeqID_122387 SeqID_122576
Ribosomal Proteins L2, RNA

SeqID_119709 SeqID_121219
binding dom

SeqID_123662 SeqID_124015

SeqID_121891

7tm_1
SeqID_119786
7 transmembrane receptor

(rhodopsin family)

Ribosomal_L3
SeqID_122659 SeqID_122727
Ribosomal protein L3

SeqID_123246 SeqID_121145

SeqID_123585

Sdh_cyt
SeqID_122442 SeqID_123881
Succinate dehydrogenase

cytochrome b subunit

DNA_topoisoIV
SeqID_120500
DNA gyrase/topoisomerase IV,

subunit A

7tm_2
SeqID_120113
7 transmembrane receptor

(Secretin family)

Ribosomal_L4
SeqID_122113 SeqID_122385
Ribosomal protein L4/L1 family

SeqID_121324 SeqID_122845

SeqID_121127 SeqID_123536

SeqID_123727

7tm_3
SeqID_120501 SeqID_120729
7 transmembrane receptor

SeqID_120999
(metabotropic gluta

Ribosomal_L5
SeqID_121970 SeqID_122399
Ribosomal protein L5

SeqID_122665 SeqID_122908

SeqID_123116 SeqID_123183

SeqID_123517 SeqID_123547

SeqID_124178

PAPS_reduct
SeqID_120874
Phosphoadenosine

phosphosulfate reductase

Ribosomal_L6
SeqID_122583 SeqID_123234
Ribosomal protein L6

SeqID_121602 SeqID_120932

SeqID_123631

ADAM_spacer1
SeqID_119479
ADAM-TS Spacer 1

HSP90
SeqID_121981 SeqID_122028
HSP90 protein

SeqID_122738 SeqID_121524

SeqID_120445 SeqID_120787

SeqID_121577 SeqID_123813

SeqID_121774 SeqID_121829

SeqID_121895

Abhydrolase_1
SeqID_119405 SeqID_121610
alpha/beta hydrolase fold

Peptidase_M1
SeqID_120743 SeqID_120851
Peptidase family M1

Herpes_LP
SeqID_120428
Herpesvirus leader protein

Pescadillo_N
SeqID_122934
Pescadillo N-terminus

Abhydrolase_3
SeqID_119940 SeqID_120282
alpha/beta hydrolase fold

SeqID_120891

CPSase_L_chain
SeqID_119260 SeqID_119619
Carbamoyl-phosphate synthase L

chain,

PMI_typeI
SeqID_122372 SeqID_123985
Phosphomannose isomerase type I

Glyco_hydro_18
SeqID_122290 SeqID_119433
Glycosyl hydrolases family 18

SeqID_123731 SeqID_124053

Profilin
SeqID_122484 SeqID_120556
Profilin

SeqID_123780 SeqID_121762

SeqID_121862

RIO1
SeqID_120808
RIO1 family

TCTP
SeqID_122571 SeqID_123113
Translationally controlled tumour

SeqID_121485 SeqID_120084
protein

SeqID_120085 SeqID_123959

NTF2
SeqID_122143 SeqID_123505
Nuclear transport factor 2 (NTF2)

SeqID_123530 SeqID_119273
domain

SeqID_120761

AP_endonuc_2
SeqID_120685
Xylose isomerase-like TIM barrel

GATase_2
SeqID_122634 SeqID_119643
Glutamine amidotransferases

SeqID_123847
class-II

RRS1
SeqID_122559 SeqID_122857
Ribosome biogenesis regulatory

SeqID_123569
protein (RRS1

Gln-synt_C
SeqID_122482 SeqID_120289
Glutamine synthetase, catalytic

domain

Pribosyltran
SeqID_122656 SeqID_122880
Phosphoribosyl transferase

SeqID_120272 SeqID_124138
domain

DUF367
SeqID_120808
Domain of unknown function

(DUF367)

PWP2
SeqID_121293
Periodic tryptophan protein 2 WD

repeat asso

RNA_pol_Rpa2_4
SeqID_119390
RNA polymerase I, Rpa2 specific

domain

HesB
SeqID_123420
HesB-like domain

SPRY
SeqID_122960 SeqID_119817
SPRY domain

COX4
SeqID_122622 SeqID_122739
Cytochrome c oxidase subunit IV

SeqID_121444 SeqID_121470

SeqID_120419 SeqID_121605

SeqID_123898 SeqID_121775

Gp-FAR-1
SeqID_122790 SeqID_123533
Nematode fatty acid retinoid

SeqID_120157 SeqID_123934
binding protein

SeqID_124215

Gln-synt_N
SeqID_122482 SeqID_123792
Glutamine synthetase, beta-Grasp

domain

Transketolase_C
SeqID_119346
Transketolase, C-terminal domain

Ctr
SeqID_119988
Ctr copper transporter family

RCC1
SeqID_122092 SeqID_120326
Regulator of chromosome

SeqID_123910
condensation (RCC1)

Pkinase_Tyr
SeqID_121233 SeqID_121271
Protein tyrosine kinase

SeqID_122086 SeqID_122117

SeqID_122221 SeqID_122233

SeqID_122285 SeqID_122384

SeqID_122470 SeqID_122618

SeqID_122687 SeqID_122758

SeqID_123026 SeqID_123421

SeqID_123491 SeqID_119148

SeqID_121409 SeqID_121416

SeqID_119206 SeqID_119219

SeqID_119238 SeqID_119245

SeqID_119296 SeqID_119338

SeqID_119410 SeqID_119412

SeqID_119422 SeqID_119436

SeqID_119453 SeqID_119472

SeqID_119483 SeqID_119520

SeqID_119525 SeqID_119541

SeqID_119728 SeqID_119764

SeqID_119789 SeqID_119828

SeqID_120006 SeqID_120051

SeqID_120126 SeqID_120166

SeqID_120298 SeqID_120406

SeqID_120418 SeqID_120428

SeqID_120452 SeqID_120453

SeqID_120550 SeqID_120636

SeqID_120663 SeqID_120672

SeqID_120772 SeqID_121622

SeqID_120934 SeqID_120996

SeqID_121189 SeqID_123672

SeqID_123838 SeqID_123977

SeqID_123989 SeqID_124109

SeqID_124294 SeqID_121714

SeqID_121742 SeqID_121803

SeqID_121897 SeqID_121907

OSCP
SeqID_122390 SeqID_123173
ATP synthase delta (OSCP)

SeqID_123852 SeqID_121694
subunit

Ham1p_like
SeqID_120320
Ham1 family

Transketolase_N
SeqID_122651 SeqID_119261
Transketolase, thiamine

SeqID_123846
diphosphate b

HD
SeqID_119514 SeqID_119787
HD domain

MreB_Mbl
SeqID_121224 SeqID_121246
MreB/Mbl protein

SeqID_121258 SeqID_121266

SeqID_122340 SeqID_119310

SeqID_120675 SeqID_123580

Fzo_mitofusin
SeqID_119682
fzo-like conserved region

GCFC
SeqID_120111
GC-rich sequence DNA-binding

factor-like pro

DER1
SeqID_123172 SeqID_120680
Der1-like family

Phosphorylase
SeqID_120862
Carbohydrate phosphorylase

SH2
SeqID_122470 SeqID_123228
SH2 domain

SeqID_119500 SeqID_120234

SeqID_120452 SeqID_120453

SeqID_120921 SeqID_120958

CXC
SeqID_121122
Tesmin/TSO1-like CXC domain

Aldedh
SeqID_120628
Aldehyde dehydrogenase family

CK_II_beta
SeqID_120267
Casein kinase II regulatory subunit

ERM
SeqID_121435 SeqID_121811
Ezrin/radixin/moesin family

3HCDH_N
SeqID_122892 SeqID_119503
3-hydroxyacyl-CoA

SeqID_120447
dehydrogenase, NAD binding

Troponin
SeqID_121972 SeqID_122915
Troponin

SeqID_123477 SeqID_120512

SeqID_123537

zf-U1
SeqID_122257 SeqID_122817
U1 zinc finger

SeqID_119881

Dynamin_M
SeqID_121459 SeqID_121020
Dynamin central region

LBP_BPI_CETP_C
SeqID_121024
LBP/BPI/CETP family, C-

terminal do

UBA
SeqID_122024 SeqID_122255
UBA/TS-N domain

SeqID_122435 SeqID_122689

SeqID_120244 SeqID_123742

SeqID_123895 SeqID_121722

SeqID_121739

Dynamin_N
SeqID_122325 SeqID_119276
Dynamin family

SeqID_119682 SeqID_121020

SeqID_123830

FG-GAP
SeqID_120791
FG-GAP repeat

Supt5
SeqID_119904
Supt5 repeat

CHORD
SeqID_122778 SeqID_121480
CHORD

SeqID_120611 SeqID_124127

Ribosomal_S6e
SeqID_122091 SeqID_122108
Ribosomal protein S6e

SeqID_123156 SeqID_123278

SeqID_123328 SeqID_123341

SeqID_123427 SeqID_119761

SeqID_123571 SeqID_124292

Gtr1_RagA
SeqID_123193 SeqID_121217
Gtr1/RagA G protein conserved

region

CAF1
SeqID_122750 SeqID_123054
CAF1 family ribonuclease

SeqID_123367 SeqID_124146

SeqID_121735

RNA_pol_Rpb6
SeqID_122713 SeqID_120963
RNA polymerase Rpb6

SeqID_124170

Hist_deacetyl
SeqID_122658 SeqID_120496
Histone deacetylase domain

SeqID_124304

RNA_pol_Rpb8
SeqID_122008 SeqID_122789
RNA polymerase Rpb8

SeqID_119393 SeqID_123747

Ribosomal_L10e
SeqID_122247 SeqID_122929
Ribosomal L10

SeqID_123115 SeqID_123302

SeqID_123354 SeqID_121429

SeqID_119804 SeqID_123702

SeqID_121800 SeqID_121851

DUF1127
SeqID_121449
Domain of unknown function

(DUF1127)

FARP
SeqID_121261 SeqID_122785
FMRFamide related peptide family

ubiquitin
SeqID_121249 SeqID_121259
Ubiquitin family

SeqID_121959 SeqID_121966

SeqID_121974 SeqID_121987

SeqID_122018 SeqID_122017

SeqID_122021 SeqID_122020

SeqID_122023 SeqID_122026

SeqID_122033 SeqID_122042

SeqID_122053 SeqID_122150

SeqID_122234 SeqID_122256

SeqID_122415 SeqID_122461

SeqID_122550 SeqID_122647

SeqID_122726 SeqID_122768

SeqID_121317 SeqID_121325

SeqID_122784 SeqID_122823

SeqID_123207 SeqID_123316

SeqID_123462 SeqID_123488

SeqID_123500 SeqID_119205

SeqID_121408 SeqID_121458

SeqID_119321 SeqID_119375

SeqID_119457 SeqID_119597

SeqID_119702 SeqID_119857

mRNA_cap_enzyme
SeqID_123044 SeqID_120040
mRNA capping enzyme, catalytic

domain

Ribosomal_60s
SeqID_122215 SeqID_122490
60s Acidic ribosomal protein

SeqID_123784 SeqID_124059

SHMT
SeqID_123444
Serine hydroxymethyltransferase

TSP_1
SeqID_121277 SeqID_119765
Thrombospondin type 1 domain

SeqID_121174

Bin3
SeqID_122035 SeqID_120646
Bicoid-interacting protein 3 (Bin3)

SeqID_123803

APS_kinase
SeqID_119710
Adenylylsulphate kinase

GSH_synthase
SeqID_122183 SeqID_119391
Eukaryotic glutathione synthase

SeqID_120121 SeqID_120409

SeqID_120622 SeqID_120719

SeqID_120886 SeqID_120895

SeqID_121074 SeqID_123719

SFT2
SeqID_123142 SeqID_121421
SFT2-like protein

SeqID_120229

Homeobox
SeqID_119358 SeqID_119397
Homeobox domain

SeqID_119915 SeqID_120364

SeqID_120535 SeqID_121140

Pox_A_type_inc
SeqID_121260 SeqID_122546
Viral A-type inclusion protein

SeqID_123618
repeat

iPGM_N
SeqID_122467 SeqID_123844
BPG-independent PGAM N-

SeqID_120794
terminus (iPGM_N)

RNA_pol_L
SeqID_122755 SeqID_120230
RNA polymerase Rpb3/Rpb11

SeqID_123595
dimerisation doma

V-set
SeqID_123529 SeqID_119196
Immunoglobulin V-set domain

SeqID_119491 SeqID_119713

SeqID_119816 SeqID_120343

CTP_synth_N
SeqID_122680 SeqID_120279
CTP synthase N-terminus

SeqID_120818 SeqID_123812

AAA
SeqID_122138 SeqID_122219
ATPase family associated with

SeqID_122276 SeqID_122358
various cellul

SeqID_122493 SeqID_122675

SeqID_121315 SeqID_121378

SeqID_123161 SeqID_119176

SeqID_121460 SeqID_119267

SeqID_119300 SeqID_119612

SeqID_119652 SeqID_119734

SeqID_119875 SeqID_120119

SeqID_120736 SeqID_121618

SeqID_120857 SeqID_120931

SeqID_120938 SeqID_123801

SeqID_123871 SeqID_124113

SeqID_124117 SeqID_121875

SeqID_121923

PP-binding
SeqID_123286 SeqID_123525
Phosphopantetheine attachment

SeqID_120461 SeqID_124213
site

SeqID_124214

CDC37
SeqID_120246
Cdc37 family

FtsJ
SeqID_120165
FtsJ-like methyltransferase

Peroxin-13_N
SeqID_119323
Peroxin 13, N-terminal

Ribosomal_S7e
SeqID_122524 SeqID_121334
Ribosomal protein S7e

SeqID_123124 SeqID_121922

Sugar_tr
SeqID_119607 SeqID_120612
Sugar (and other) transporter

UCH
SeqID_122246 SeqID_122435
Ubiquitin carboxyl-terminal

SeqID_122511 SeqID_121433
hydrolase

SeqID_119366 SeqID_119785

SeqID_119799 SeqID_120244

SeqID_120577 SeqID_121090

SeqID_123828 SeqID_123895

SeqID_121784

HATPase_c
SeqID_121965 SeqID_121968
Histidine kinase-, DNA gyrase B-,

SeqID_121976 SeqID_122028
and HSP90

SeqID_122252 SeqID_123282

SeqID_124089 SeqID_120445

SeqID_123704

Activin_recp
SeqID_123267
Activin types I and II receptor

domain

DUF602
SeqID_119868
Protein of unknown function,

DUF602

DUF1136
SeqID_119196
Repeat of unknown function

(DUF1136)

TAFII28
SeqID_123141
hTAFII28-like protein conserved

region

Pkinase
SeqID_121233 SeqID_121271
Protein kinase domain

SeqID_122086 SeqID_122117

SeqID_122221 SeqID_122233

SeqID_122285 SeqID_122384

SeqID_122470 SeqID_122502

SeqID_122618 SeqID_122687

SeqID_122758 SeqID_122956

SeqID_123026 SeqID_123491

SeqID_119148 SeqID_121409

SeqID_121416 SeqID_121441

SeqID_119219 SeqID_119237

SeqID_119238 SeqID_119245

SeqID_119296 SeqID_119338

SeqID_119365 SeqID_119410

SeqID_119412 SeqID_119436

SeqID_119453 SeqID_119472

SeqID_119483 SeqID_119520

SeqID_119525 SeqID_119576

SeqID_119629 SeqID_119728

SeqID_119764 SeqID_119789

SeqID_119828 SeqID_119968

SeqID_120006 SeqID_120126

SeqID_120166 SeqID_120208

SeqID_120298 SeqID_120406

SeqID_120428 SeqID_120452

SeqID_120453 SeqID_120550

SeqID_120636 SeqID_120663

SeqID_120672 SeqID_120701

SeqID_120771 SeqID_120772

SeqID_121561 SeqID_121583

SeqID_121622 SeqID_120796

SeqID_120934 SeqID_120996

SeqID_121102 SeqID_121189

SeqID_123672 SeqID_123947

SeqID_123977 SeqID_123989

SeqID_124109 SeqID_124130

SeqID_124294 SeqID_121714

SeqID_121742 SeqID_121803

SeqID_121897 SeqID_121903

SeqID_121907 SeqID_121920

KH_1
SeqID_122074 SeqID_122460
KH domain

SeqID_121313 SeqID_123392

SeqID_123476 SeqID_119195

SeqID_120972 SeqID_124132

SeqID_121787

FA_hydroxylase
SeqID_122136 SeqID_123602
Fatty acid hydroxylase

Clc-like
SeqID_122446 SeqID_120339
Clc-like

SeqID_120721 SeqID_123875

KH_2
SeqID_122074 SeqID_122370
KH domain

SeqID_123179 SeqID_121535

SeqID_121549 SeqID_123699

SeqID_124132 SeqID_124172

SeqID_124328 SeqID_121787

Galactosyl_T_2
SeqID_122573 SeqID_122681
Galactosyltransferase

SeqID_119322 SeqID_124349

Piwi
SeqID_121243 SeqID_121305
Piwi domain

SeqID_123411 SeqID_119347

SeqID_119676 SeqID_120129

SeqID_121773 SeqID_121792

RLI
SeqID_122076 SeqID_120449
Possible metal-binding domain in

SeqID_120808 SeqID_124131
RNase L inh

HORMA
SeqID_121286 SeqID_119567
HORMA domain

SeqID_121728

RNA_pol_Rpb1_3
SeqID_120039 SeqID_120437
RNA polymerase Rpb1, domain 3

SeqID_120506

Ldh_2
SeqID_122223 SeqID_121410
Malate/L-lactate dehydrogenase

SeqID_121556 SeqID_121574

SeqID_124002

Neuralized
SeqID_122352 SeqID_120365
Neuralized

SeqID_124000 SeqID_124184

RNA_pol_Rpb1_4
SeqID_120039 SeqID_120437
RNA polymerase Rpb1, domain 4

SeqID_120506 SeqID_120541

RNA_pol_Rpb1_5
SeqID_120506
RNA polymerase Rpb1, domain 5

RNA_pol_Rpb1_6
SeqID_120506
RNA polymerase Rpb1, domain 6

Clat_adaptor_s
SeqID_122102 SeqID_122224
Clathrin adaptor complex small

SeqID_122801 SeqID_122988
chain

SeqID_120385 SeqID_120859

SeqID_121131 SeqID_123790

SeqID_124166

IF4E
SeqID_122412 SeqID_120589
Eukaryotic initiation factor 4E

Kinesin
SeqID_121296 SeqID_122362
Kinesin motor domain

SeqID_119669 SeqID_124181

G10
SeqID_122515
G10 protein

Ground-like
SeqID_120751
Ground-like domain

P34-Arc
SeqID_123215 SeqID_121855
Arp2/3 complex, 34 kD subunit

p34-Arc

Ribosomal_S8e
SeqID_122003 SeqID_122010
Ribosomal protein S8e

SeqID_122130 SeqID_122508

SeqID_121333 SeqID_123027

SeqID_121508 SeqID_121531

SeqID_121545 SeqID_123644

SeqID_121667

Ribosomal_S3_C
SeqID_122370 SeqID_123179
Ribosomal protein S3, C-terminal

SeqID_119376 SeqID_121535
domai

SeqID_121126 SeqID_123699

ResIII
SeqID_122318 SeqID_119170
Type III restriction enzyme, res

SeqID_121443 SeqID_119233
subunit

SeqID_119766 SeqID_120168

SeqID_123835 SeqID_124119

TFIIE_beta
SeqID_121012
TFIIE beta subunit core domain

AA_kinase
SeqID_122430
Amino acid kinase family

Exo_endo_phos
SeqID_122447 SeqID_123874
Endonuclease/Exonuclease/phosphatase

fa

HLH
SeqID_122206 SeqID_119868
Helix-loop-helix DNA-binding

SeqID_120631
domain

Keratin_B2
SeqID_120481
Keratin, high sulfur B2 protein

TspO_MBR
SeqID_122231 SeqID_122587
TspO/MBR family

SeqID_123338 SeqID_121431

SeqID_119499 SeqID_123778

SeqID_123779 SeqID_124344

SeqID_121908

C1-set
SeqID_119491 SeqID_120343
Immunoglobulin C1-set domain

SCO1-SenC
SeqID_121873
SCO1/SenC

T-box
SeqID_122717 SeqID_123320
T-box

PSI
SeqID_119510
Plexin repeat

AAA_2
SeqID_122219 SeqID_122276
ATPase family associated with

SeqID_121378 SeqID_119652
various cellul

SeqID_119734 SeqID_121618

SeqID_120931 SeqID_123871

SeqID_124113 SeqID_121923

DUF477
SeqID_122322 SeqID_124018
Domain of unknown function

(DUF477)

AAA_3
SeqID_122493 SeqID_119176
ATPase family associated with

SeqID_119742 SeqID_119875
various cellul

SeqID_120649 SeqID_121618

ABC_membrane
SeqID_120493
ABC transporter transmembrane

region

fn3
SeqID_119158 SeqID_119225
Fibronectin type III domain

SeqID_119569 SeqID_120170

SeqID_120951 SeqID_121023

AAA_5
SeqID_122219 SeqID_122493
ATPase family associated with

SeqID_119176 SeqID_119875
various cellul

SeqID_120649 SeqID_120763

SeqID_120931 SeqID_120936

SeqID_123871

Destabilase
SeqID_122806 SeqID_124205
Destabilase

Glyco_transf_22
SeqID_123460 SeqID_120564
Alg9-like mannosyltransferase

family

Not3
SeqID_122203 SeqID_121162
Not1 N-terminal domain, CCR4-

Not complex com

CDC50
SeqID_122266 SeqID_121335
LEM3 (ligand-effect modulator 3)

SeqID_120160 SeqID_123808
family/CD

Glyco_transf_25
SeqID_121052
Glycosyltransferase family 25 (LPS

bi

PSS
SeqID_119991
Phosphatidyl serine synthase

PRP38
SeqID_123506 SeqID_121713
PRP38 family

UCR_14kD
SeqID_122477 SeqID_121481
Ubiquinol-cytochrome C reductase

SeqID_121514 SeqID_123716
complex 14k

Biopterin_H
SeqID_122189 SeqID_122692
Biopterin-dependent aromatic

SeqID_122958 SeqID_120567
amino acid h

SeqID_123832 SeqID_124293

Cofilin_ADF
SeqID_122563 SeqID_122620
Cofilin/tropomyosin-type actin-

SeqID_121306 SeqID_121419
binding pr

SeqID_119918 SeqID_124332

MOZ_SAS
SeqID_123033
MOZ/SAS family

SNase
SeqID_121415 SeqID_120155
Staphylococcal nuclease

homologue

Skp1_POZ
SeqID_121262 SeqID_122339
Skp1 family, tetramerisation

SeqID_122672 SeqID_121318
domain

SeqID_119994 SeqID_123642

Acyl_transf_3
SeqID_119415
Acyltransferase family

Ribosomal_L10
SeqID_123472 SeqID_120199
Ribosomal protein L10

SeqID_121812

HMA
SeqID_122636 SeqID_123900
Heavy-metal-associated domain

Ribosomal_S3Ae
SeqID_122253 SeqID_123202
Ribosomal S3Ae family

SeqID_119906 SeqID_121492

SeqID_123962

Ribosomal_L11
SeqID_122466 SeqID_122612
Ribosomal protein L11, RNA

SeqID_122798 SeqID_120200
binding do

SeqID_121603 SeqID_120902

SeqID_123787 SeqID_121653

SeqID_124218

eIF-1a
SeqID_122566 SeqID_123523
Eukaryotic initiation factor 1A

SeqID_119774 SeqID_124173

Ribosomal_L13e
SeqID_121985 SeqID_121989
Ribosomal protein L13e

SeqID_122082 SeqID_119425

SeqID_123942

Ribosomal_L12
SeqID_122236 SeqID_121124
Ribosomal protein L7/L12 C-

SeqID_123921
terminal dom

S10_plectin
SeqID_122608 SeqID_119224
Plectin/S10 domain

SeqID_123551

Ribosomal_L14
SeqID_122293 SeqID_123957
Ribosomal protein L14p/L23e

DUF625
SeqID_120752
Protein of unknown function

(DUF625)

Sec23_trunk
SeqID_121381 SeqID_119647
Sec23/Sec24 trunk domain

ig
SeqID_123529 SeqID_119196
Immunoglobulin domain

SeqID_119491 SeqID_119569

SeqID_119713 SeqID_119816

SeqID_120343

Ribosomal_L16
SeqID_122247 SeqID_122653
Ribosomal protein L16

SeqID_123302 SeqID_124211

Ion_trans
SeqID_119930 SeqID_120812
Ion transport protein

NAF1
SeqID_119903
NAF1 domain

Aa_trans
SeqID_121971 SeqID_119531
Transmembrane amino acid

transporter protein

APG6
SeqID_122694 SeqID_121500
Autophagy protein Apg6

SEC-C
SeqID_120562
SEC-C motif

KE2
SeqID_122599 SeqID_122773
KE2 family protein

SeqID_124174 SeqID_124230

SeqID_124235

Lyase_1
SeqID_120948 SeqID_121661
Lyase

SeqID_121786

Ran_BP1
SeqID_119729 SeqID_120815
RanBP1 domain

PGM_PMM_IV
SeqID_119474 SeqID_120700
Phosphoglucomutase/phosphomannomutase,

C-t

BAH
SeqID_123089
BAH domain

UQ_con
SeqID_121983 SeqID_122089
Ubiquitin-conjugating enzyme

SeqID_122437 SeqID_122519

SeqID_122757 SeqID_123232

ENTH
SeqID_122942 SeqID_123389
ENTH domain

DUF6
SeqID_119240
Integral membrane protein DUF6

Ribosomal_L21e
SeqID_122144 SeqID_123108
Ribosomal protein L21e

SeqID_123268 SeqID_123293

SeqID_123298 SeqID_121434

SeqID_119745 SeqID_123751

SeqID_124341 SeqID_121876

Cyclin_C
SeqID_120722
Cyclin, C-terminal domain

ADK
SeqID_121282 SeqID_122317
Adenylate kinase

SeqID_119710

MAS20
SeqID_121991 SeqID_122410
MAS20 protein import receptor

SeqID_123936

TIG
SeqID_122994
IPT/TIG domain

DNA_pol_B
SeqID_120301
DNA polymerase family B

Ribosomal_L22
SeqID_121995 SeqID_122261
Ribosomal protein L22p/L17e

SeqID_122840 SeqID_123164

SeqID_123257 SeqID_121516

SeqID_120038 SeqID_123674

Ribosomal_L14e
SeqID_122701 SeqID_123548
Ribosomal protein L14

Ribosomal_L23
SeqID_122038 SeqID_123046
Ribosomal protein L23

SeqID_119380 SeqID_119408

SeqID_123694 SeqID_124231

SNF2_N
SeqID_119766 SeqID_120168
SNF2 family N-terminal domain

SeqID_120323

Cgr1
SeqID_122506 SeqID_123609
Cgr1 family

Glutaredoxin
SeqID_123129
Glutaredoxin

PUA
SeqID_122707 SeqID_119847
PUA domain

tRNA_m1G_MT_9
SeqID_122151 SeqID_123209
tRNA m(1)G methyltransferase

RNA_pol_Rpb2_3
SeqID_123375 SeqID_121089
RNA polymerase Rpb2, domain 3

Ribosomal_L29
SeqID_122312 SeqID_122610
Ribosomal L29 protein

SeqID_123285 SeqID_119200

SeqID_123638 SeqID_123687

SeqID_121725

RNA_pol_Rpb2_4
SeqID_123375 SeqID_121089
RNA polymerase Rpb2, domain 4

zf-nanos
SeqID_121133
Nanos RNA binding domain

RNA_pol_Rpb2_5
SeqID_122280 SeqID_119390
RNA polymerase Rpb2, domain 5

SeqID_121089 SeqID_124072

Peptidase_S8
SeqID_121230 SeqID_123401
Subtilase family

SeqID_119631

PUF
SeqID_122211 SeqID_122825
Pumilio-family RNA binding repeat

SeqID_119161 SeqID_121389

SeqID_123824 SeqID_121723

RNA_pol_Rpb2_6
SeqID_122280 SeqID_122552
RNA polymerase Rpb2, domain 6

SeqID_121330 SeqID_123182

SeqID_119390 SeqID_123587

SeqID_123831

Cyclin_N
SeqID_122428 SeqID_122670
Cyclin, N-terminal domain

SeqID_122816 SeqID_121486

SeqID_120722 SeqID_121612

SeqID_123768 SeqID_123905

Mod_r
SeqID_123251 SeqID_123409
Modifier of rudimentary (Mod(r))

SeqID_120718 SeqID_123566
protein

RNA_pol_Rpb2_7
SeqID_122552
RNA polymerase Rpb2, domain 7

Ribosomal_L7Ae
SeqID_121269 SeqID_122134
Ribosomal protein

SeqID_122180 SeqID_122335
L7Ae/L30e/S12e/Gadd4

SeqID_122367 SeqID_122404

SeqID_122604 SeqID_122718

SeqID_123155 SeqID_123294

SeqID_123455 SeqID_119560

SeqID_123888 SeqID_119859

SeqID_121504 SeqID_123633

SeqID_123660 SeqID_123698

SeqID_123927 SeqID_123935

SeqID_123992 SeqID_124144

POLO_box
SeqID_119828 SeqID_121022
POLO box duplicated region

Nucleoporin2
SeqID_120026
Nucleoporin autopeptidase

zf-BED
SeqID_119515
BED zinc finger

Ets
SeqID_119450
Ets-domain

Ribosomal_S2
SeqID_122439 SeqID_122525
Ribosomal protein S2

SeqID_122923 SeqID_122995

SeqID_123127 SeqID_123308

SeqID_123382 SeqID_120337

SeqID_120668 SeqID_123668

SeqID_124044

Rcd1
SeqID_121351 SeqID_119153
Cell differentiation family, Rcd1-

like

Ribosomal_S4
SeqID_121533 SeqID_121546
Ribosomal protein S4/S9 N-

terminal domai

GMC_oxred_C
SeqID_122311
GMC oxidoreductase

Ribosomal_S5
SeqID_122366 SeqID_123421
Ribosomal protein S5, N-terminal

SeqID_123632
domai

DUF1240
SeqID_120294
Protein of unknown function

(DUF1240)

Topoisom_I
SeqID_123167 SeqID_121520
Eukaryotic DNA topoisomerase I,

SeqID_121660 SeqID_121884
catalytic

Ribosomal_S6
SeqID_122928 SeqID_119470
Ribosomal protein S6

DUF1241
SeqID_122262 SeqID_120893
Protein of unknown function

(DUF1241)

Ribosomal_S7
SeqID_122487 SeqID_122488
Ribosomal protein S7p/S5e

SeqID_122733 SeqID_123383

SeqID_124307 SeqID_123688

SeqID_124272

Ssl1
SeqID_122605 SeqID_123911
Ssl1-like

SeqID_124066 SeqID_121741

Ribosomal_S8
SeqID_122779 SeqID_120839
Ribosomal protein S8

Nop52
SeqID_122283 SeqID_120786
Nucleolar protein, Nop52

SeqID_121201 SeqID_124065

Ribosomal_L22e
SeqID_122791 SeqID_120882
Ribosomal L22e protein family

Ribosomal_L30
SeqID_121300 SeqID_122251
Ribosomal protein L30p/L7e

SeqID_122565 SeqID_123737

SeqID_123736 SeqID_121777

SeqID_121894 SeqID_121896

AdoHcyase
SeqID_122459 SeqID_123859
S-adenosyl-L-homocysteine

hydrolase

Ribosomal_L15e
SeqID_121969 SeqID_122199
Ribosomal L15

SeqID_123299 SeqID_119488

SeqID_119941 SeqID_120030

SeqID_123763 SeqID_123764

SeqID_124342

V-ATPase_C
SeqID_120905
V-ATPase subunit C

Proteasome
SeqID_121422 SeqID_121276
Proteasome A-type and B-type

SeqID_121284 SeqID_121960

SeqID_121962 SeqID_122045

SeqID_122055 SeqID_122190

SeqID_122396 SeqID_122413

SeqID_122474 SeqID_122498

SeqID_121342 SeqID_122780

SeqID_122803 SeqID_123336

SeqID_123362 SeqID_123419

SeqID_119278 SeqID_119909

SeqID_121468 SeqID_120588

SeqID_120924 SeqID_123604

SeqID_123611 SeqID_123646

SeqID_123681 SeqID_123825

SeqID_124136 SeqID_124227

SeqID_121889

GMC_oxred_N
SeqID_122311 SeqID_124037
GMC oxidoreductase

PHF5
SeqID_122542 SeqID_120850
PHF5-like protein

SeqID_123639

DNA_gyraseB
SeqID_120500
DNA gyrase B

Cullin
SeqID_122420 SeqID_119796
Cullin family

SeqID_119797 SeqID_120045

SeqID_123756 SeqID_120756

SeqID_120773 SeqID_121682

SeqID_121780 SeqID_121941

DUF572
SeqID_119715 SeqID_120330
Family of unknown function

(DUF572)

FAA_hydrolase
SeqID_122355 SeqID_124011
Fumarylacetoacetate (FAA)

hydrolase fam

cNMP_binding
SeqID_119563 SeqID_121604
Cyclic nucleotide-binding domain

V-ATPase_G
SeqID_122706 SeqID_123291
Vacuolar (H+)-ATPase G subunit

SeqID_119894 SeqID_119927

SeqID_123872

V-ATPase_H
SeqID_120190
V-ATPase subunit H

Epimerase
SeqID_119815 SeqID_120284
NAD dependent

SeqID_121143
epimerase/dehydratase family

Lipase_2
SeqID_119177
Lipase (class 2)

Ribosomal_L39
SeqID_122520 SeqID_122799
Ribosomal L39 protein

HCNGP
SeqID_120697 SeqID_120698
HCNGP-like protein

POP1
SeqID_123216
Ribonucleases P/MRP protein

subunit POP1

SMN
SeqID_122580 SeqID_124165
Survival motor neuron protein

(SMN)

ACPS
SeqID_122149 SeqID_124163
4′-phosphopantetheinyl transferase

superfami

Lamp
SeqID_122690 SeqID_123937
Lysosome-associated membrane

glycoprotein (L

FragX_IP
SeqID_120422
Cytoplasmic Fragile-X interacting

family

Aminotran_1_2
SeqID_122344
Aminotransferase class I and II

ABC_tran
SeqID_122402 SeqID_119524
ABC transporter

SeqID_119625 SeqID_120769

SeqID_123949

GRP
SeqID_119747 SeqID_120570
Glycine rich protein family

Vps54
SeqID_122919 SeqID_119956
Vps54-like protein

Aph-1
SeqID_122155 SeqID_123059
Aph-1 protein

SeqID_124013

Radical_SAM
SeqID_120962
Radical SAM superfamily

Josephin
SeqID_122683 SeqID_123774
Josephin

SeqID_123928

EF1G
SeqID_122057 SeqID_123454
Elongation factor 1 gamma,

SeqID_120547 SeqID_123557
conserved domain

Monooxygenase
SeqID_122333 SeqID_123771
Monooxygenase

EXS
SeqID_120654
EXS family

PCNA_C
SeqID_122062 SeqID_122819
Proliferating cell nuclear antigen,

SeqID_120167
C-termin

Sad1_UNC
SeqID_119813 SeqID_120250
Sad1/UNC-like C-terminal

AMP-binding
SeqID_123152 SeqID_120233
AMP-binding enzyme

DIM1
SeqID_123449 SeqID_124285
Mitosis protein DIM1

ATP_bind_1
SeqID_122375 SeqID_122533
Conserved hypothetical ATP

SeqID_122872 SeqID_119782
binding protein

SeqID_123655 SeqID_123983

DUF652
SeqID_122800 SeqID_119259
Protein of unknown function,

SeqID_123553 SeqID_124216
DUF652

PCNA_N
SeqID_122062 SeqID_122819
Proliferating cell nuclear antigen,

SeqID_122829 SeqID_121061
N-termin

SeqID_124283

DUF727
SeqID_122615 SeqID_119316
Protein of unknown function

SeqID_121702 SeqID_124046
(DUF727)

Utp11
SeqID_122295 SeqID_121156
Utp11 protein

ThiF
SeqID_121273 SeqID_122346
ThiF family

SeqID_119266 SeqID_121104

SeqID_124189

MMR_HSR1
SeqID_121288 SeqID_122204
GTPase of unknown function

SeqID_122360 SeqID_122375

SeqID_122392 SeqID_122397

SeqID_122560 SeqID_122575

SeqID_122579 SeqID_122600

SeqID_122619 SeqID_122657

SeqID_122671 SeqID_121329

SeqID_122792 SeqID_122872

SeqID_123422 SeqID_119204

SeqID_121430 SeqID_119311

SeqID_119332 SeqID_119352

SeqID_119367 SeqID_119492

SeqID_119523 SeqID_119645

SeqID_119791 SeqID_120413

SeqID_120523 SeqID_120766

SeqID_121613 SeqID_120900

SeqID_121038 SeqID_121093

SeqID_123568 SeqID_123726

SeqID_123740 SeqID_123754

SeqID_121709 SeqID_123960

SeqID_123950 SeqID_123988

SeqID_123991 SeqID_124180

SeqID_121717 SeqID_121740

zf-C2H2
SeqID_122179 SeqID_122249
Zinc finger, C2H2 type

SeqID_122267 SeqID_123033

SeqID_123091 SeqID_123110

SeqID_123191 SeqID_119156

SeqID_119250 SeqID_119283

SeqID_119315 SeqID_119326

SeqID_119455 SeqID_119515

SeqID_119638 SeqID_119674

SeqID_119915 SeqID_119925

SeqID_120046 SeqID_120117

SeqID_120617 SeqID_120707

SeqID_121133 SeqID_121172

SeqID_123906 SeqID_123914

SeqID_124145 SeqID_121781

SeqID_121822

HEAT
SeqID_122200 SeqID_122532
HEAT repeat

SeqID_121374 SeqID_121380

SeqID_122870 SeqID_119166

SeqID_121402 SeqID_119389

SeqID_119427 SeqID_119658

SeqID_124126 SeqID_119790

SeqID_119852 SeqID_119929

SeqID_121537 SeqID_119998

SeqID_120055 SeqID_120163

SeqID_120538 SeqID_120549

SeqID_120581 SeqID_120656

SeqID_121550 SeqID_121614

SeqID_121006 SeqID_121175

SeqID_121201 SeqID_123600

SeqID_121708

PWI
SeqID_122736 SeqID_122749
PWI domain

SeqID_120835 SeqID_124267

SeqID_124270

Syja_N
SeqID_119649 SeqID_120216
Sacl homology domain

zf-Sec23_Sec24
SeqID_120593
Sec23/Sec24 zinc finger

Gcd10p
SeqID_122163 SeqID_119505
Gcd10p family

SeqID_124154

Gelsolin
SeqID_119286 SeqID_121187
Gelsolin repeat

FUN14
SeqID_122589
FUN14 family

UcrQ
SeqID_122007 SeqID_122808
UcrQ family

SeqID_119696 SeqID_124290

Ribosomal_L31e
SeqID_122378 SeqID_123337
Ribosomal protein L31e

SeqID_123982

Ribosomal_L24e
SeqID_122132 SeqID_123616
Ribosomal protein L24e

SeqID_123617 SeqID_124161

Calreticulin
SeqID_122265 SeqID_122837
Calreticulin family

SeqID_119911 SeqID_123897

eIF-5a
SeqID_122554 SeqID_123204
Eukaryotic initiation factor 5A

SeqID_120378 SeqID_123582
hypusine, DN

Pex14_N
SeqID_122174 SeqID_123766
Peroxisomal membrane anchor

protein (Pex14p)

DUF663
SeqID_123123 SeqID_120018
Protein of unknown function

(DUF663)

UIM
SeqID_122683 SeqID_123389
Ubiquitin interaction motif

SeqID_123774

COX5A
SeqID_122529 SeqID_120371
Cytochrome c oxidase subunit Va

SeqID_123670

COX5B
SeqID_121281 SeqID_122395
Cytochrome c oxidase subunit Vb

SeqID_122479 SeqID_121428

SeqID_119688 SeqID_123743

SeqID_124156

Ribosomal_L23eN
SeqID_122038 SeqID_123046
Ribosomal protein L23, N-terminal

SeqID_119380 SeqID_119408
dom

SeqID_123694

PH
SeqID_121274 SeqID_123067
PH domain

SeqID_119258 SeqID_119463

SeqID_120842 SeqID_120929

SeqID_121878

GTP_EFTU_D2
SeqID_122391 SeqID_122657
Elongation factor Tu domain 2

SeqID_121299 SeqID_122792

SeqID_123334 SeqID_123345

SeqID_119311 SeqID_119352

SeqID_121538 SeqID_121551

SeqID_120413 SeqID_120540

SeqID_123754 SeqID_123950

SeqID_123967 SeqID_121805

SeqID_121825 SeqID_121826

Sas10_Utp3
SeqID_120054
Sas10/Utp3 family

Prp18
SeqID_123355 SeqID_120394
Prp18 domain

GTP_EFTU_D3
SeqID_122729 SeqID_122792
Elongation factor Tu C-terminal

SeqID_123334 SeqID_123345
domain

SeqID_121538 SeqID_121551

SeqID_120413 SeqID_123754

SeqID_121664 SeqID_121825

SeqID_121826 SeqID_121939

GATA
SeqID_121080
GATA zinc finger

Spectrin
SeqID_119246 SeqID_119482
Spectrin repeat

SeqID_119575 SeqID_120539

SeqID_120627

V-SNARE
SeqID_122066 SeqID_121095
Vesicle transport v-SNARE protein

SeqID_123939

Ribosomal_S5_C
SeqID_122366 SeqID_123632
Ribosomal protein S5, C-terminal

SeqID_121839
domai

PX
SeqID_120853
PX domain

KID
SeqID_120763
KID repeat

GSH_synth_ATP
SeqID_122183 SeqID_121436
Eukaryotic glutathione synthase,

SeqID_120021 SeqID_120101
ATP bi

SeqID_120121 SeqID_120409

SeqID_120622 SeqID_120666

SeqID_120719 SeqID_120782

SeqID_120886 SeqID_120895

SeqID_121074 SeqID_123719

MCM
SeqID_119742 SeqID_119912
MCM2/3/5 family

SeqID_120936

ETF_alpha
SeqID_121607
Electron transfer flavoprotein alpha

subuni

L51_S25_CI-B8
SeqID_122048 SeqID_123231
Mitochondrial ribosomal protein

SeqID_121114 SeqID_123645
L51/S

CBS
SeqID_120735 SeqID_121213
CBS domain

Ribosomal_L18e
SeqID_122342 SeqID_122374
Eukaryotic ribosomal protein L18

SeqID_123713 SeqID_123753

SeqID_121703 SeqID_124289

OTCace
SeqID_119435
Aspartate/omithine

carbamoyltransterase, A

GRAM
SeqID_122481 SeqID_123794
GRAM domain

SeqID_121636

Rad21_Rec8
SeqID_122963
Conserved region of Rad21/Rec8

like prot

DUF676
SeqID_120421
Putative serine esterase (DUF676)

Ribosomal_L18p
SeqID_120495 SeqID_121687
Ribosomal L18p/L5e family

Metallophos
SeqID_122191 SeqID_122213
Calcineurin-like phosphoesterase

SeqID_122289 SeqID_123311

SeqID_123307 SeqID_121426

SeqID_119337 SeqID_119448

SeqID_119502 SeqID_119572

SeqID_119659 SeqID_119751

SeqID_119860 SeqID_121540

SeqID_120128 SeqID_120238

SeqID_120434 SeqID_120484

SeqID_120553 SeqID_120710

SeqID_120825 SeqID_120824

SeqID_120834 SeqID_120873

SeqID_121046 SeqID_121142

SeqID_121188 SeqID_121700

SeqID_124029 SeqID_124030

SeqID_124061 SeqID_121738

SeqID_121746 SeqID_121770

SeqID_121899 SeqID_121900

SeqID_121915

HECT
SeqID_121283 SeqID_121316
HECT-domain (ubiquitin-

SeqID_119865 SeqID_120854
transferase)

SeqID_121657 SeqID_121712

Hormone_recep
SeqID_120699 SeqID_121758
Ligand-binding domain of nuclear

hormon

NAC
SeqID_122153 SeqID_122689
NAC domain

SeqID_123502 SeqID_120888

SeqID_123742

C1_1
SeqID_120222 SeqID_120526
Phorbol esters/diacylglycerol

binding domain

Calponin
SeqID_121222 SeqID_122248
Calponin family repeat

SeqID_122936 SeqID_123005

SeqID_123171 SeqID_123188

SeqID_123400 SeqID_120392

SeqID_120928 SeqID_120927

SeqID_123804 SeqID_123842

SeqID_123853 SeqID_124187

RmaAD
SeqID_122723 SeqID_119487
Ribosomal RNA adenine

SeqID_123996
dimethylase

SPX
SeqID_120654
SPX domain

C1_3
SeqID_119379 SeqID_120217
C1-like domain

SeqID_120788

GST_C
SeqID_122057 SeqID_122407
Glutathione S-transferase, C-

SeqID_122628 SeqID_122642
terminal domain

SeqID_121383 SeqID_123011

SeqID_121623 SeqID_121132

SeqID_123557 SeqID_123625

SeqID_123709 SeqID_123708

SeqID_124298 SeqID_121838

Na_Ca_ex
SeqID_120053
Sodium/calcium exchanger protein

B3_4
SeqID_120243
B3/4 domain

Sec23_helical
SeqID_119286
Sec23/Sec24 helical domain

Ribosomal_L40e
SeqID_122033 SeqID_121408
Ribosomal L40e family

SeqID_119857 SeqID_123721

ICIn_channel
SeqID_121998 SeqID_123531
Nucleotide-sensitive chloride

conductanc

Histone
SeqID_121234 SeqID_122039
Core histone H2A/H2B/H3/H4

SeqID_122046 SeqID_122096

SeqID_122119 SeqID_122455

SeqID_122483 SeqID_122582

SeqID_122688 SeqID_122744

SeqID_122766 SeqID_122771

SeqID_121314 SeqID_122884

SeqID_122955 SeqID_123003

SeqID_123317 SeqID_123463

SeqID_123466 SeqID_123473

SeqID_121448 SeqID_121446

SeqID_119330 SeqID_119493

SeqID_119512 SeqID_119521

SeqID_119609 SeqID_119776

SeqID_119983 SeqID_121479

SeqID_121482 SeqID_120068

SeqID_120077 SeqID_120480

SeqID_120534 SeqID_120790

SeqID_121570 SeqID_121594

SeqID_121601 SeqID_120813

SeqID_121054 SeqID_123744

SeqID_121645 SeqID_121663

SeqID_121675 SeqID_121683

SeqID_121684 SeqID_121688

SeqID_121706 SeqID_121705

SeqID_124105 SeqID_124164

SeqID_124258 SeqID_124262

SeqID_124276 SeqID_124314

SeqID_121771 SeqID_121809

SeqID_121819 SeqID_121828

SeqID_121823 SeqID_121905

Disintegrin
SeqID_120481
Disintegrin

3HCDH
SeqID_120447
3-hydroxyacyl-CoA

dehydrogenase, C-terminal

NAP
SeqID_122303 SeqID_119982
Nucleosome assembly protein

SeqID_124050
(NAP)

Tubulin
SeqID_122269 SeqID_122332
Tubulin/FtsZ family, GTPase

SeqID_122440 SeqID_121336
domain

SeqID_123013 SeqID_119381

SeqID_119720 SeqID_124114

SeqID_121499 SeqID_121506

SeqID_121543 SeqID_120775

SeqID_121555 SeqID_123545

SeqID_123884 SeqID_124069

SeqID_121761 SeqID_121820

SeqID_121849 SeqID_121893

GST_N
SeqID_122057 SeqID_122407
Glutathione S-transferase, N-

SeqID_122628 SeqID_122642
terminal domain

SeqID_121383 SeqID_123011

SeqID_123274 SeqID_119360

SeqID_121496 SeqID_120438

SeqID_121623 SeqID_123557

SeqID_123625 SeqID_123709

SeqID_123708 SeqID_124297

SeqID_121838

ETC_C1_NDUFA5
SeqID_122406 SeqID_123873
ETC complex I subunit conserved

region

2-Hacid_dh
SeqID_120275
D-isomer specific 2-hydroxyacid

dehydrogen

Adenylsucc_synt
SeqID_122272 SeqID_121355
Adenylosuccinate synthetase

SeqID_119162 SeqID_119650

SeqID_120090 SeqID_120822

SeqID_124006

RTC
SeqID_122518 SeqID_123729
RNA 3′-terminal phosphate cyclase

Ribosomal_L19e
SeqID_122613 SeqID_122869
Ribosomal protein L19e

SeqID_123303 SeqID_120779

TRAPP_Bet3
SeqID_122875
Transport protein particle (TRAPP)

compone

SMC_C
SeqID_119625
SMC family, C-terminal domain

CDP-OH_P_transf
SeqID_123356
CDP-alcohol

phosphatidyltransferase

Frataxin_Cyay
SeqID_122075 SeqID_123508
Frataxin-like domain

SeqID_120847 SeqID_124330

VHS
SeqID_120912
VHS domain

DUF689
SeqID_122204
Protein of unknown function

(DUF689)

SMC_N
SeqID_119887 SeqID_119966
RecF/RecN/SMC N terminal

SeqID_120574 SeqID_120702
domain

PTPLA
SeqID_120767
Protein tyrosine phosphatase-like

protein, P

PfkB
SeqID_122553 SeqID_123583
pfkB family carbohydrate kinase

DSPc
SeqID_122616 SeqID_122746
Dual specificity phosphatase,

SeqID_124323 SeqID_119299
catalytic doma

SeqID_119527 SeqID_120116

SeqID_123836 SeqID_124056

Biotin_lipoyl
SeqID_120976
Biotin-requiring enzyme

Pkinase_C
SeqID_122758 SeqID_122978
Protein kinase C terminal domain

SeqID_119365 SeqID_121625

SeqID_120796

DAD
SeqID_119716
DAD family

Alpha_adaptin_C
SeqID_120950
Alpha adaptin AP2, C-terminal

domain

Ribosomal_L6e
SeqID_122489 SeqID_122949
Ribosomal protein L6e

SeqID_121011 SeqID_123683

S1
SeqID_122067 SeqID_123689
S1 RNA binding domain

SeqID_123690

Oxidored_q6
SeqID_121980 SeqID_122154
NADH ubiquinone oxidoreductase,

SeqID_119667 SeqID_124082
20 Kd sub

Extensin_2
SeqID_120572 SeqID_120593
Extensin-like region

SeqID_120713

Gar1
SeqID_119747
Gar1 protein RNA binding region

S4
SeqID_122623 SeqID_121533
S4 domain

SeqID_121546 SeqID_120098

SeqID_124001

Bromodomain
SeqID_119794
Bromodomain

Laminin_N
SeqID_119297 SeqID_119335
Laminin N-terminal (Domain VI)

CDI
SeqID_122878
Cyclin-dependent kinase inhibitor

Mago_nashi
SeqID_123521 SeqID_120904
Mago nashi protein

SNF7
SeqID_122586 SeqID_119719
SNF7

SeqID_119746 SeqID_120690

SeqID_121136 SeqID_124098

ShTK
SeqID_122581 SeqID_119601
ShTK domain

SeqID_120015 SeqID_120669

tRNA_anti
SeqID_122633 SeqID_120602
OB-fold nucleic acid binding

SeqID_124327
domain

Linker_histone
SeqID_122931 SeqID_120058
linker histone H1 and H5 family

DAO
SeqID_122333 SeqID_122338
FAD dependent oxidoreductase

SeqID_122411 SeqID_120662

SeqID_121620 SeqID_123933

NDUF_B7
SeqID_120001 SeqID_122009
NADH-ubiquinone oxidoreductase

SeqID_122777 SeqID_123519
B18 subunit (

SeqID_123938 SeqID_124080

Ribosomal_L34e
SeqID_122631 SeqID_123111
Ribosomal protein L34e

SeqID_123332 SeqID_119760

SeqID_123896

DUF906
SeqID_119497
Domain of Unknown Function

(DUF906)

SPC12
SeqID_122753 SeqID_121566
Microsomal signal peptidase 12 kDa

SeqID_121590 SeqID_124248
subunit (

CLN3
SeqID_123346 SeqID_120607
CLN3 protein

SeqID_120681

RVT_1
SeqID_119187 SeqID_119374
Reverse transcriptase (RNA-

SeqID_119417 SeqID_119451
dependent DNA pol

SeqID_119461 SeqID_119462

SeqID_119508 SeqID_119566

SeqID_119662 SeqID_119663

SeqID_119834 SeqID_119855

SeqID_119943 SeqID_120004

SeqID_120103 SeqID_120138

SeqID_120352 SeqID_120433

SeqID_120465 SeqID_120471

SeqID_120546 SeqID_120566

SeqID_120586 SeqID_120687

SeqID_120716 SeqID_120852

SeqID_120935 SeqID_121043

SeqID_121058 SeqID_121128

SeqID_121129 SeqID_121179

Gp_dh_C
SeqID_121978 SeqID_122147
Glyceraldehyde 3-phosphate

SeqID_121379 SeqID_121400
dehydrogenase, C-

SeqID_121457 SeqID_119718

SeqID_123607

Ldi_recept_b
SeqID_119985 SeqID_120019
Low-density lipoprotein receptor

repeat

F_actin_cap_B
SeqID_122095 SeqID_123254
F-actin capping protein, beta

SeqID_123555
subunit

Methyltransf_8
SeqID_122377 SeqID_119368
Hypothetical methyltransferase

SeqID_123981

Mt_ATP-synt_B
SeqID_122761 SeqID_120653
Mitochondrial ATP synthase B

SeqID_124245 SeqID_121789
chain prec

KAP_NTPase
SeqID_120368
KAP family P-loop domain

Mt_ATP-synt_D
SeqID_122314 SeqID_123912
ATP synthase D chain,

mitochondrial (AT

SAC3_GANP
SeqID_121169
SAC3/GANP family

Gp_dh_N
SeqID_121986 SeqID_122098
Glyceraldehyde 3-phosphate

SeqID_122147 SeqID_123064
dehydrogenase, NA

SeqID_123079 SeqID_123357

SeqID_123457 SeqID_121379

SeqID_121457 SeqID_123840

SeqID_123607 SeqID_123870

SeqID_124339 SeqID_121930

An_peroxidase
SeqID_120092 SeqID_120241
Animal haem peroxidase

SeqID_120906

Ephrin
SeqID_119824
Ephrin

polyprenyl_synt
SeqID_120635
Polyprenyl synthetase

Neur_chan_memb
SeqID_121005 SeqID_121087
Neurotransmitter-gated ion-

channel tra

zf-NPL4
SeqID_123465 SeqID_119522
NPL4 family, putative zinc binding

region

XAP5
SeqID_122814 SeqID_122850
XAP5 protein

SeqID_121167

RNA_pol
SeqID_120255
DNA-dependent RNA polymerase

NMT_C
SeqID_121407 SeqID_120382
Myristoyl-CoA: protein N-

myristoyltransferase

Aldose_epim
SeqID_122507 SeqID_120204
Aldose 1-epimerase

SeqID_123603

DUF841
SeqID_120243
Eukaryotic protein of unknown

function (DUF8

Mov34
SeqID_121290 SeqID_121294
Mov34/MPN/PAD-1 family

SeqID_122120 SeqID_122260

SeqID_122735 SeqID_121344

SeqID_122844 SeqID_123535

SeqID_121382 SeqID_121417

SeqID_119999 SeqID_120711

SeqID_123682 SeqID_121693

SeqID_124040 SeqID_124087

SeqID_121736

NAD_binding_1
SeqID_122444 SeqID_123823
Oxidoreductase NAD-binding

domain

Ribosomal_L28e
SeqID_122648 SeqID_120682
Ribosomal L28e protein family

SeqID_123720

LIM
SeqID_122321 SeqID_123039
LIM domain

SeqID_123158 SeqID_123185

SeqID_119388 SeqID_119555

SeqID_119701 SeqID_120218

SeqID_123816 SeqID_121638

SeqID_124168

SPC25
SeqID_122737 SeqID_124254
Microsomal signal peptidase 25 kDa

subunit (

WGR
SeqID_121544
WGR domain

STT3
SeqID_120152
Oligosaccharyl transferase STT3

subun

WH2
SeqID_119377
WH2 motif

14-3-3
SeqID_122166 SeqID_122862
14-3-3 protein

SeqID_123514 SeqID_119268

SeqID_119536 SeqID_119605

Alpha_adaptinC2
SeqID_120950
Adaptin C-terminal domain

CbiA
SeqID_122680
CobQ/CobB/MinD/ParA nucleotide

binding do

zf-MIZ
SeqID_120096
MIZ zinc finger

Lipocalin
SeqID_122101 SeqID_122172
Lipocalin/cytosolic fatty-acid

SeqID_123222 SeqID_119931
binding pr

SeqID_120164 SeqID_123572

SeqID_123598 SeqID_121698

DLIC
SeqID_122981
Dynein light intermediate chain

(DLIC)

tRNA-synt_1c_C
SeqID_120890
tRNA synthetases class I (E and

Q), an

Bestrophin
SeqID_119636
Bestrophin

eIF-3_zeta
SeqID_122685 SeqID_120237
Eukaryotic translation initiation

SeqID_123560
factor 3

Porin_3
SeqID_122330 SeqID_121328
Eukaryotic porin

SeqID_119190 SeqID_121557

SeqID_121575 SeqID_123999

ARID
SeqID_120573
ARID/BRIGHT DNA binding

domain

CybS
SeqID_122786 SeqID_124202
CybS

BCAS2
SeqID_122510 SeqID_123556
Breast carcinoma amplified

sequence 2 (BCAS2

Motile_Sperm
SeqID_122721 SeqID_123310
MSP (Major sperm protein) domain

SeqID_119174 SeqID_119175

SeqID_119281 SeqID_119604

SeqID_119641 SeqID_119724

SeqID_120035 SeqID_120095

SeqID_120327 SeqID_120441

SeqID_120517 SeqID_120563

SeqID_120632 SeqID_120633

SeqID_120910 SeqID_124175

SeqID_121850

Transket_pyr
SeqID_122651 SeqID_119346
Transketolase, pyridine binding

SeqID_120804 SeqID_120844
domai

SeqID_123846

Fibrillarin
SeqID_123301 SeqID_119795
Fibrillarin

PABP
SeqID_122526 SeqID_121469
Poly-adenylate binding protein,

SeqID_123820 SeqID_121869
unique domai

BRCT
SeqID_121491 SeqID_120459
BRCA1 C Terminus (BRCT)

SeqID_121621
domain

Psf2
SeqID_119632
Partner of SLD five, PSF2

tRNA-synt_1
SeqID_123431 SeqID_119678
tRNA synthetases class I (I, L, M

SeqID_121502
and V)

Psf3
SeqID_122668 SeqID_123586
Partner of SLD five, PSF3

tRNA-synt_2
SeqID_122301 SeqID_124123
tRNA synthetases class II (D, K

SeqID_119833 SeqID_124052
and N)

SeqID_121567 SeqID_121591

SeqID_121045

NDK
SeqID_121977 SeqID_122294
Nucleoside diphosphate kinase

SeqID_121345 SeqID_123201

SeqID_123452 SeqID_123451

SeqID_121388 SeqID_123559

ATP-synt_DE_N
SeqID_122751 SeqID_121401
ATP synthase, Delta/Epsilon

SeqID_124264 SeqID_123752
chain, beta

zf-C4
SeqID_122310 SeqID_122617
Zinc-finger, C4 type (two domains)

SeqID_122812 SeqID_123080

SeqID_123445 SeqID_119666

SeqID_120699 SeqID_120820

SeqID_124333

DIRP
SeqID_119217
DIRP

Ribosomal_L36e
SeqID_122112 SeqID_123552
Ribosomal protein L36e

Filament
SeqID_121260 SeqID_122400
Intermediate filament protein

SeqID_122546 SeqID_123386

SeqID_119327 SeqID_121473

SeqID_120922 SeqID_123579

SeqID_123618 SeqID_123953

TFIID_30kDa
SeqID_120863
Transcription initiation factor TFIID

23-

DUF926
SeqID_120692
Domain of Unknown Function

(DUF926)

DUF854
SeqID_119289 SeqID_120777

Caenorhabditis elegans repeat of

unknown fun

TPP_enzyme_M
SeqID_120595
Thiamine pyrophosphate enzyme,

central d

PPI_Ypi1
SeqID_122389 SeqID_120052
Protein phosphatase inhibitor

SeqID_123885

Myosin_head
SeqID_119861
Myosin head (motor domain)

MH1
SeqID_122197 SeqID_123448
MH1 domain

SeqID_121021

RWD
SeqID_122537 SeqID_120442
RWD domain

DUF858
SeqID_122961
Eukaryotic protein of unknown

function (DUF8

3Beta_HSD
SeqID_123272 SeqID_121396
3-beta hydroxysteroid

SeqID_119630 SeqID_121143
dehydrogenase/isomera

BIR
SeqID_123175 SeqID_119442
Inhibitor of Apoptosis domain

SeqID_121665

MTHFR
SeqID_122188 SeqID_120829
Methylenetetrahydrofolate

SeqID_124115
reductase

GYF
SeqID_121994
GYF domain

E1_dh
SeqID_122564 SeqID_122651
Dehydrogenase E1 component

SeqID_123263 SeqID_123821

SeqID_123846

Fork_head
SeqID_120305
Fork head domain

DUF1604
SeqID_119654
Protein of unknown function

(DUF1604)

OST3_OST6
SeqID_123025
OST3/OST6 family

Cadherin
SeqID_119235 SeqID_119705
Cadherin domain

PPTA
SeqID_122418 SeqID_123390
Protein prenyltransferase alpha

SeqID_121057 SeqID_123924
subunit repe

GCV_H
SeqID_122462 SeqID_123213
Glycine cleavage H-protein

SeqID_119530

Aldolase_II
SeqID_121238 SeqID_122401
Class II Aldolase and Adducin N-

SeqID_122499 SeqID_121462
terminal

SeqID_121475 SeqID_123767

SeqID_121689 SeqID_123952

SeqID_121934

AIG1
SeqID_119367
AIG1 family

RNase_PH
SeqID_122716 SeqID_122887
3′ exoribonuclease family, domain 1

SeqID_120679 SeqID_124199

Ribosomal_L18ae
SeqID_122646 SeqID_122664
Ribosomal L18ae protein family

SeqID_122858 SeqID_123114

SeqID_123121 SeqID_120878

SeqID_123624 SeqID_123886

SeqID_124236 SeqID_121791

SeqID_121797 SeqID_121925

Nucleoside_tran
SeqID_123441 SeqID_124095
Nucleoside transporter

Ribosomal_L37e
SeqID_122591 SeqID_121170
Ribosomal protein L37e

SeqID_123963

Prefoldin
SeqID_120211 SeqID_123679
Prefoldin subunit

Beta-lactamase
SeqID_122907 SeqID_120056
Beta-lactamase

PC_rep
SeqID_122969
Proteasome/cyclosome repeat

DEAD
SeqID_122207 SeqID_122277
DEAD/DEAH box helicase

SeqID_122318 SeqID_122319

SeqID_122381 SeqID_123007

SeqID_123018 SeqID_119170

SeqID_121414 SeqID_121443

SeqID_119233 SeqID_119256

SeqID_119300 SeqID_119324

SeqID_119657 SeqID_119712

SeqID_119766 SeqID_120168

SeqID_120342 SeqID_120509

SeqID_120575 SeqID_121032

SeqID_121200 SeqID_123835

SeqID_123976 SeqID_124021

SeqID_124074 SeqID_124119

SURF4
SeqID_120816
SURF4 family

NCD3G
SeqID_120999
Nine Cysteines Domain of family 3

GPCR

SURF6
SeqID_122863
Surfeit locus protein 6

Sec10
SeqID_120206
Exocyst complex component

Sec10

Oxidored_molyb
SeqID_122426 SeqID_123908
Oxidoreductase molybdopterin

binding d

Cation_efflux
SeqID_119547 SeqID_119854
Cation efflux family

HisKA_2
SeqID_121848
Histidine kinase

RNA_pol_Rpb5_C
SeqID_122441 SeqID_123528
RNA polymerase Rpb5, C-terminal

SeqID_123883
domain

dUTPase
SeqID_120762
dUTPase

Calx-beta
SeqID_120919
Calx-beta domain

FA_desaturase
SeqID_122220 SeqID_122258
Fatty acid desaturase

BRF1
SeqID_119280
Brf1-like TBP-binding domain

W2
SeqID_119265 SeqID_121487
eIF4-gamma/eIF5/eIF2-epsilon

PIP5K
SeqID_123028
Phosphatidylinositol-4-phosphate

5-Kinase

Ribosomal_L35Ae
SeqID_122114 SeqID_122827
Ribosomal protein L35Ae

SeqID_123435 SeqID_123889

SeqID_124288

RTC_insert
SeqID_122518 SeqID_120776
RNA 3′-terminal phosphate cyclase

SeqID_123973
(RTC), i

SKIP_SNW
SeqID_121033
SKIP/SNW domain

PAP_assoc
SeqID_122124 SeqID_123088
PAP/25A associated domain

SeqID_120195

DNA_pol_E_B
SeqID_122568 SeqID_124351
DNA polymerase epsilon subunit B

RNA_pol_Rpb5_N
SeqID_122441 SeqID_123137
RNA polymerase Rpb5, N-terminal

SeqID_123528 SeqID_119977
domain

SeqID_123883

Vicilin_N
SeqID_121108
Vicilin N terminal region

DEP
SeqID_120527
Domain found in Dishevelled, Egl-

10, and Ple

Cytochrom_C
SeqID_122036 SeqID_123651
Cytochrome c

Ribosomal_L38e
SeqID_122040 SeqID_119735
Ribosomal L38e protein family

SeqID_123834

GRIM-19
SeqID_123149 SeqID_119221
GRIM-19 protein

SeqID_121885

DUF947
SeqID_120569
Domain of unknown function

(DUF947)

DnaJ
SeqID_121245 SeqID_122232
DnaJ domain

SeqID_122521 SeqID_122541

SeqID_122667 SeqID_122722

SeqID_119277 SeqID_119288

SeqID_119644 SeqID_120879

SeqID_120983 SeqID_121206

SeqID_123640 SeqID_123715

SeqID_123882 SeqID_124112

SeqID_124311

G6PD_C
SeqID_122743 SeqID_124271
Glucose-6-phosphate

dehydrogenase, C-termina

PHO4
SeqID_122752 SeqID_120810
Phosphate transporter family

SeqID_124256

Reprolysin
SeqID_120801
Reprolysin (M12B) family zinc

metalloprote

MIT
SeqID_120407
MIT domain

LRR_1
SeqID_122148 SeqID_122601
Leucine Rich Repeat

SeqID_119275 SeqID_119350

SeqID_120299 SeqID_120639

SeqID_121187 SeqID_123576

SeqID_123887

Ribosomal_S21e
SeqID_123512 SeqID_119578
Ribosomal protein S21e

SeqID_123570

tRNA-synt_1d_C
SeqID_122724
DALR anticodon binding domain

RNA_pol_A_bac
SeqID_120230 SeqID_121039
RNA polymerase Rpb3/RpoA

insert domain

KOW
SeqID_122127 SeqID_122516
KOW motif

SeqID_122554 SeqID_122701

SeqID_122710 SeqID_123295

SeqID_123458 SeqID_119904

SeqID_123548 SeqID_123582

SeqID_123634 SeqID_123734

ECH
SeqID_122281 SeqID_122454
Enoyl-CoA hydratase/isomerase

SeqID_119372 SeqID_123865
family

SeqID_124071 SeqID_121747

IF_tail
SeqID_121260 SeqID_122400
Intermediate filament tail domain

SeqID_120920 SeqID_123579

PAN
SeqID_120695
PAN domain

zf-C3HC4
SeqID_122347 SeqID_122496
Zinc finger, C3HC4 type (RING

SeqID_123384 SeqID_123511
finger)

SeqID_119241 SeqID_119357

SeqID_120644 SeqID_123692

SeqID_124075

WW
SeqID_122257 SeqID_122416
WW domain

SeqID_119608 SeqID_120846

SeqID_120854 SeqID_123538

PB1
SeqID_120217
PB1 domain

NOG1
SeqID_119367
Nucleolar GTP-binding protein 1

(NOG1)

PAS
SeqID_122206
PAS domain

EI24
SeqID_121718
Etoposide-induced protein 2.4

(EI24)

MIF4G
SeqID_120041
MIF4G domain

PI3_PI4_kinase
SeqID_119291 SeqID_119922
Phosphatidylinositol 3- and 4-

SeqID_120521 SeqID_121582
kinase

SeqID_120853 SeqID_121831

PAZ
SeqID_121243 SeqID_119347
PAZ domain

SeqID_119944 SeqID_121539

SeqID_120129 SeqID_121552

SeqID_121086 SeqID_121913

Cpn60_TCP1
SeqID_122080 SeqID_122245
TCP-1/cpn60 chaperonin family

SeqID_123243 SeqID_123369

SeqID_119577 SeqID_119896

SeqID_121517 SeqID_120191

SeqID_120292 SeqID_120613

SeqID_120876 SeqID_121091

SeqID_123615 SeqID_123735

SeqID_121655 SeqID_123955

Tim17
SeqID_122097 SeqID_124320
Tim17/Tim22/Tim23 family

SeqID_121063

Ligase_CoA
SeqID_122725 SeqID_124060
CoA-ligase

Trehalase
SeqID_121616
Trehalase

PQ-loop
SeqID_122891 SeqID_123245
PQ loop repeat

SeqID_123591

TTL
SeqID_119185
Tubulin-tyrosine ligase family

Myb_DNA-binding
SeqID_119288 SeqID_119345
Myb-like DNA-binding domain

SeqID_120201 SeqID_121080

SeqID_121197

Ribonuclease_3
SeqID_121044
RNase3 domain

Ribophorin_I
SeqID_121320 SeqID_120333
Ribophorin I

SeqID_121666

CAS_CSE1
SeqID_119961
CAS/CSE protein, C-terminus

Pex2_Pex12
SeqID_122740 SeqID_123980
Pex2/Pex12 amino terminal

region

Table 1 Legend:

Column 1 - pfam name or designation

Column 2 - gene family member listed by SEQ ID NO corresponding to amino acid sequence translation from vcDNA SEQ ID NO identified in feature field of peptide sequence

Column 3 - Protein annotation based on BLASTP comparisons

In order to construct a dsRNA sequence, or concatamers or chimeras of dsRNA sequences from various genes either within SCN, from other pest nucleotide sequences, or a combination thereof, nucleotide sequences corresponding to the SCN genome sequences were BLASTed against known vertebrate, soybean, and Rhizobium nucleotide sequences to first eliminate contiguous sequences in SCN that matched sequences in known vertebrate, soybean, and Rhozobium sequences that are at least about 21 nucleotides in length. This redacted set of SCN sequences was then compared to known nucleotide sequences in parasitic nematodes, insects, and fungi to identify sequences of substantial identity that could be useful in constructing sequences that, when expressed as a dsRNA sequence, are capable of effecting gene suppression in SCN as well as in another parasitic nematode, or insect, or fungal pest. The results of comparisons to other parasitic nematode sequences are shown in Table 2.

TABLE 2

Diverse Parasitic Nematode Nucleotide Coding Sequences

Matching H. glycines vcDNA sequences

H. glycines

Sequence¹
Position²
GeneID³
Position⁴
% identity⁵

Seq ID NO: 50950
21-138
gi|28916076
1-119
91%

Seq ID NO: 50950
14-41
gi|159473
34-61
100%

Seq ID NO: 50950
14-41
gi|2454547
227-200
100%

Seq ID NO: 50950
14-41
gi|551594
194-221
100%

Seq ID NO: 50950
14-41
gi|551595
669-696
100%

Seq ID NO: 50950
14-41
gi|18032254
34-61
100%

Seq ID NO: 50950
18-41
gi|18477256
279-256
100%

Seq ID NO: 50950
18-41
gi|18477260
325-302
100%

Seq ID NO: 50950
18-41
gi|18477262
634-611
100%

Seq ID NO: 50950
14-35
gi|18477259
22-1
100%

Seq ID NO: 50950
14-35
gi|18477261
22-1
100%

Seq ID NO: 50950
14-35
gi|37780968
22-1
100%

Seq ID NO: 50973
88-108
gi|19267657
465-445
100%

Seq ID NO: 51012
302-411
gi|18080245
178-69
84%

Seq ID NO: 51132
182-360
gi|32323955
5-183
96%

Seq ID NO: 51132
184-359
gi|33139778
6-182
96%

Seq ID NO: 51132
182-351
gi|33140274
5-174
95%

Seq ID NO: 51132
195-360
gi|32324510
1-166
95%

Seq ID NO: 51132
218-354
gi|33140264
1-137
95%

Seq ID NO: 51132
182-284
gi|32324219
5-107
94%

Seq ID NO: 51164
117-137
gi|22543645
32-12
100%

Seq ID NO: 51169
1-236
gi|47118285
740-975
97%

Seq ID NO: 51169
1-236
gi|47118286
753-988
97%

Seq ID NO: 51169
1-219
gi|16797830
731-950
97%

Seq ID NO: 51169
1-219
gi|21885260
751-969
97%

Seq ID NO: 51169
1-219
gi|26000759
727-945
97%

Seq ID NO: 51169
1-219
gi|31442322
728-946
97%

Seq ID NO: 51169
1-219
gi|31442320
728-946
96%

Seq ID NO: 51169
4-219
gi|16797831
732-947
96%

Seq ID NO: 51169
1-219
gi|38096133
730-948
94%

Seq ID NO: 51169
1-33
gi|16797827
776-807
96%

Seq ID NO: 51169
131-159
gi|16797844
862-890
96%

Seq ID NO: 51169
216-236
gi|48479719
1112-1132
100%

Seq ID NO: 51184
16-47
gi|32324419
271-302
100%

Seq ID NO: 51378
184-459
gi|32325335
36-311
97%

Seq ID NO: 51378
504-626
gi|32325335
311-433
100%

Seq ID NO: 51378
753-841
gi|32325335
453-541
97%

Seq ID NO: 51378
101-139
gi|32325335
1-39
100%

Seq ID NO: 51378
676-702
gi|32325335
430-456
100%

Seq ID NO: 51561
402-476
gi|33139584
80-6
100%

Seq ID NO: 51561
192-229
gi|33139584
115-78
100%

Seq ID NO: 51561
192-215
gi|6969910
34-11
100%

Seq ID NO: 51579
1-138
gi|33139914
209-346
97%

Seq ID NO: 51823
55-187
gi|18081060
80-212
86%

Seq ID NO: 51824
3-97
gi|54548889
100-194
87%

Seq ID NO: 51824
3-97
gi|18089540
421-515
85%

Seq ID NO: 51824
3-79
gi|18081060
404-480
87%

Seq ID NO: 51978
1-314
gi|33139071
85-397
96%

Seq ID NO: 51978
307-395
gi|33139071
406-494
97%

Seq ID NO: 51978
406-452
gi|33139071
505-551
97%

Seq ID NO: 51978
406-475
gi|32325389
507-576
97%

Seq ID NO: 51978
1-237
gi|33140521
87-323
94%

Seq ID NO: 51991
239-260
gi|30028357
64-85
100%

Seq ID NO: 52051
1-78
gi|33140098
496-573
100%

Seq ID NO: 52051
1-74
gi|32324423
496-569
100%

Seq ID NO: 52051
1-61
gi|33139933
499-559
100%

Seq ID NO: 52051
1-58
gi|33139533
495-552
100%

Seq ID NO: 52051
56-168
gi|18082418
136-248
85%

Seq ID NO: 52162
163-185
gi|40670360
123-101
100%

Seq ID NO: 52240
388-410
gi|38513673
44-22
100%

Seq ID NO: 52285
172-204
gi|18090560
293-325
100%

Seq ID NO: 52293
1-133
gi|54549200
31-163
91%

Seq ID NO: 52293
11-133
gi|7144024
38-160
91%

Seq ID NO: 52293
30-133
gi|18090416
1-104
91%

Seq ID NO: 52293
11-133
gi|54545022
9-131
88%

Seq ID NO: 52293
41-133
gi|54545591
1-93
91%

Seq ID NO: 52311
1-75
gi|30028941
235-309
96%

Seq ID NO: 52311
1-75
gi|33140204
235-309
96%

Seq ID NO: 52432
1-102
gi|35504996
472-574
96%

Seq ID NO: 52432
1-58
gi|30028727
523-580
100%

Seq ID NO: 52432
1-54
gi|30028246
523-576
100%

Seq ID NO: 52432
1-39
gi|35505048
461-499
100%

Seq ID NO: 52438
179-210
gi|18383056
1007-976
100%

Seq ID NO: 52569
1-311
gi|32324631
49-359
95%

Seq ID NO: 52596
77-218
gi|30028020
420-279
97%

Seq ID NO: 52642
79-324
gi|33139639
249-493
86%

Seq ID NO: 52642
66-218
gi|33140673
201-49
91%

Seq ID NO: 52642
286-342
gi|32324211
207-263
91%

Seq ID NO: 52710
25-152
gi|33140374
1-128
94%

Seq ID NO: 52762
1-90
gi|34105813
1098-1009
98%

Seq ID NO: 52762
1-90
gi|2707748
1096-1007
97%

Seq ID NO: 52762
1-90
gi|51093962
1079-990
97%

Seq ID NO: 52762
1-90
gi|31376322
1040-952
95%

Seq ID NO: 52762
1-90
gi|31074278
1105-1017
94%

Seq ID NO: 52762
1-90
gi|31376323
1036-948
94%

Seq ID NO: 52762
1-90
gi|6983959
1059-971
94%

Seq ID NO: 52762
3-90
gi|31074279
1103-1017
94%

Seq ID NO: 52762
1-87
gi|34105806
1106-1020
94%

Seq ID NO: 52762
1-87
gi|30844180
1105-1019
93%

Seq ID NO: 52762
1-90
gi|30525812
1092-1002
92%

Seq ID NO: 52762
1-90
gi|31376331
1031-942
92%

Seq ID NO: 52762
1-90
gi|54873771
134-44
92%

Seq ID NO: 52762
1-64
gi|34105810
1104-1041
98%

Seq ID NO: 52831
1-47
gi|32324245
540-586
100%

Seq ID NO: 52831
1-40
gi|33139824
507-546
100%

Seq ID NO: 52831
2-101
gi|18089712
314-413
84%

Seq ID NO: 52831
1-29
gi|33140223
507-535
100%

Seq ID NO: 52831
2-52
gi|7143499
320-371
86%

Seq ID NO: 52831
1-23
gi|33140453
507-529
100%

Seq ID NO: 52854
153-174
gi|21378611
322-301
100%

Seq ID NO: 52965
21-138
gi|28916076
1-119
91%

Seq ID NO: 52965
14-41
gi|159473
34-61
100%

Seq ID NO: 52965
14-41
gi|2454547
227-200
100%

Seq ID NO: 52965
14-41
gi|551594
194-221
100%

Seq ID NO: 52965
14-41
gi|551595
669-696
100%

Seq ID NO: 52965
14-41
gi|18032254
34-61
100%

Seq ID NO: 52965
18-41
gi|18477256
279-256
100%

Seq ID NO: 52965
18-41
gi|18477260
325-302
100%

Seq ID NO: 52965
18-41
gi|18477262
634-611
100%

Seq ID NO: 52965
14-35
gi|18477259
22-1
100%

Seq ID NO: 52965
14-35
gi|18477261
22-1
100%

Seq ID NO: 52965
14-35
gi|37780968
22-1
100%

Seq ID NO: 52976
617-707
gi|32325459
205-290
83%

Seq ID NO: 52981
15-425
gi|33140136
92-502
87%

Seq ID NO: 53038
793-899
gi|18090533
269-375
89%

Seq ID NO: 53058
469-594
gi|18081946
320-195
90%

Seq ID NO: 53058
172-199
gi|1766021
225-252
96%

Seq ID NO: 53058
172-199
gi|7641281
173-200
96%

Seq ID NO: 53110
73-218
gi|33139131
99-244
89%

Seq ID NO: 53110
79-218
gi|32324211
1-140
88%

Seq ID NO: 53110
68-218
gi|33140673
199-49
85%

Seq ID NO: 53110
86-218
gi|33139639
256-388
84%

Seq ID NO: 53110
288-314
gi|33139639
467-493
96%

Seq ID NO: 53113
15-54
gi|28916076
1-40
97%

Seq ID NO: 53113
8-35
gi|159473
34-61
100%

Seq ID NO: 53113
8-35
gi|2454547
227-200
100%

Seq ID NO: 53113
8-35
gi|551594
194-221
100%

Seq ID NO: 53113
8-35
gi|551595
669-696
100%

Seq ID NO: 53113
8-35
gi|18032254
34-61
100%

Seq ID NO: 53113
12-35
gi|18477256
279-256
100%

Seq ID NO: 53113
12-35
gi|18477260
325-302
100%

Seq ID NO: 53113
12-35
gi|18477262
634-611
100%

Seq ID NO: 53113
8-29
gi|18477259
22-1
100%

Seq ID NO: 53113
8-29
gi|18477261
22-1
100%

Seq ID NO: 53113
8-29
gi|37780968
22-1
100%

Seq ID NO: 53345
33-68
gi|32324562
547-512
97%

Seq ID NO: 53381
122-150
gi|18382995
332-304
96%

Seq ID NO: 53444
20-42
gi|8005191
86-64
100%

Seq ID NO: 53584
1-223
gi|33139862
41-263
99%

Seq ID NO: 53584
266-445
gi|33139862
262-441
99%

Seq ID NO: 53584
1-129
gi|32325297
437-565
100%

Seq ID NO: 53584
601-702
gi|33139953
453-554
99%

Seq ID NO: 53584
1-146
gi|22544160
265-410
86%

Seq ID NO: 53584
767-855
gi|7143567
305-217
88%

Seq ID NO: 53584
1-128
gi|30167496
229-356
82%

Seq ID NO: 53771
15-54
gi|28916076
1-40
97%

Seq ID NO: 53771
8-35
gi|159473
34-61
100%

Seq ID NO: 53771
8-35
gi|2454547
227-200
100%

Seq ID NO: 53771
8-35
gi|551594
194-221
100%

Seq ID NO: 53771
8-35
gi|551595
669-696
100%

Seq ID NO: 53771
8-35
gi|18032254
34-61
100%

Seq ID NO: 53771
12-35
gi|18477256
279-256
100%

Seq ID NO: 53771
12-35
gi|18477260
325-302
100%

Seq ID NO: 53771
12-35
gi|18477262
634-611
100%

Seq ID NO: 53771
8-29
gi|18477259
22-1
100%

Seq ID NO: 53771
8-29
gi|18477261
22-1
100%

Seq ID NO: 53771
8-29
gi|37780968
22-1
100%

Seq ID NO: 53788
196-219
gi|19265811
51-74
100%

Seq ID NO: 53903
49-125
gi|51334373
320-396
90%

Seq ID NO: 53903
281-309
gi|7143754
460-488
100%

Seq ID NO: 53903
281-309
gi|54546250
453-481
100%

Seq ID NO: 53942
1-127
gi|33140348
254-128
87%

Seq ID NO: 53996
95-170
gi|18080046
260-334
90%

Seq ID NO: 54067
614-700
gi|18080124
72-159
84%

Seq ID NO: 54067
630-658
gi|18082691
129-157
96%

Seq ID NO: 54223
1-32
gi|18082819
494-463
100%

Seq ID NO: 54223
1-32
gi|32324738
389-420
100%

Seq ID NO: 54223
1-32
gi|54546964
145-176
100%

Seq ID NO: 54228
9-154
gi|33139017
5-150
99%

Seq ID NO: 54361
453-568
gi|33140744
29-141
84%

Seq ID NO: 54394
96-242
gi|33140673
195-49
90%

Seq ID NO: 54394
110-281
gi|33139639
256-428
88%

Seq ID NO: 54404
1-41
gi|18381736
808-848
90%

Seq ID NO: 54404
19-63
gi|18382098
510-554
89%

Seq ID NO: 54404
19-41
gi|18381713
520-542
100%

Seq ID NO: 54411
69-177
gi|18090579
65-176
86%

Seq ID NO: 54431
127-151
gi|18089765
337-361
100%

Seq ID NO: 45816
323-510
gi|52128303
201-388
81%

Seq ID NO: 54533
295-336
gi|7144329
64-105
97%

Seq ID NO: 54598
17-91
gi|33139131
116-190
92%

Seq ID NO: 54598
6-97
gi|32324211
1-92
87%

Seq ID NO: 54650
161-185
gi|32183975
527-551
100%

Seq ID NO: 54664
1-99
gi|33139292
225-323
100%

Seq ID NO: 54709
1-91
gi|6562543
742-832
98%

Seq ID NO: 54709
22-59
gi|18382129
349-386
92%

Seq ID NO: 54710
1-112
gi|18090579
65-176
90%

Seq ID NO: 54782
21-138
gi|28916076
1-119
91%

Seq ID NO: 54782
14-41
gi|159473
34-61
100%

Seq ID NO: 54782
14-41
gi|2454547
227-200
100%

Seq ID NO: 54782
14-41
gi|551594
194-221
100%

Seq ID NO: 54782
14-41
gi|551595
669-696
100%

Seq ID NO: 54782
14-41
gi|18032254
34-61
100%

Seq ID NO: 54782
18-41
gi|18477256
279-256
100%

Seq ID NO: 54782
18-41
gi|18477260
325-302
100%

Seq ID NO: 54782
18-41
gi|18477262
634-611
100%

Seq ID NO: 54782
14-35
gi|18477259
22-1
100%

Seq ID NO: 54782
14-35
gi|18477261
22-1
100%

Seq ID NO: 54782
14-35
gi|37780968
22-1
100%

Seq ID NO: 54811
73-94
gi|7641297
281-302
100%

Seq ID NO: 54839
194-282
gi|33139570
594-506
100%

Seq ID NO: 54839
198-282
gi|30029553
597-513
100%

Seq ID NO: 54839
131-282
gi|18079986
658-507
88%

Seq ID NO: 54839
218-282
gi|33139565
577-513
100%

Seq ID NO: 54839
132-220
gi|21493943
587-499
89%

Seq ID NO: 54839
232-282
gi|32324861
563-513
100%

Seq ID NO: 54839
240-282
gi|32324744
548-506
97%

Seq ID NO: 54847
3-101
gi|28916076
176-79
89%

Seq ID NO: 54865
54-202
gi|33140673
199-51
88%

Seq ID NO: 54865
61-202
gi|33139639
245-386
88%

Seq ID NO: 54865
59-199
gi|33139131
99-239
86%

Seq ID NO: 45847
183-308
gi|18080348
231-356
85%

Seq ID NO: 45847
190-308
gi|7144294
198-316
85%

Seq ID NO: 54946
128-285
gi|54546846
415-257
85%

Seq ID NO: 54981
83-126
gi|551594
1028-1070
93%

Seq ID NO: 54981
83-126
gi|551595
267-309
93%

Seq ID NO: 54999
328-498
gi|18382277
801-971
82%

Seq ID NO: 55025
372-395
gi|33138439
263-286
100%

Seq ID NO: 55025
419-439
gi|33952838
448-428
100%

Seq ID NO: 55106
21-138
gi|28916076
1-119
91%

Seq ID NO: 55106
14-41
gi|159473
34-61
100%

Seq ID NO: 55106
14-41
gi|2454547
227-200
100%

Seq ID NO: 55106
14-41
gi|551594
194-221
100%

Seq ID NO: 55106
14-41
gi|551595
669-696
100%

Seq ID NO: 55106
14-41
gi|18032254
34-81
100%

Seq ID NO: 55106
18-41
gi|18477256
279-256
100%

Seq ID NO: 55106
18-41
gi|18477260
325-302
100%

Seq ID NO: 55106
18-41
gi|18477262
634-611
100%

Seq ID NO: 55106
14-35
gi|18477259
22-1
100%

Seq ID NO: 55106
14-35
gi|18477261
22-1
100%

Seq ID NO: 55106
14-35
gi|37780968
22-1
100%

Seq ID NO: 55108
1-93
gi|7143864
42-134
82%

Seq ID NO: 55117
35-57
gi|7144284
325-303
100%

Seq ID NO: 55119
36-155
gi|28916076
140-20
90%

Seq ID NO: 55175
1-160
gi|5107411
189-30
100%

Seq ID NO: 55175
1-160
gi|2149587
189-30
95%

Seq ID NO: 55175
47-160
gi|34105813
1719-1606
100%

Seq ID NO: 55175
1-160
gi|2149585
189-30
92%

Seq ID NO: 55175
1-160
gi|2738785
190-30
92%

Seq ID NO: 55175
1-160
gi|2738792
190-30
92%

Seq ID NO: 55175
1-160
gi|2738799
190-30
92%

Seq ID NO: 55175
1-160
gi|2738800
190-30
92%

Seq ID NO: 55175
2-160
gi|2707748
1766-1607
90%

Seq ID NO: 55175
1-80
gi|48479719
191-112
98%

Seq ID NO: 55175
1-80
gi|37674501
194-115
98%

Seq ID NO: 55175
115-160
gi|37674501
75-30
95%

Seq ID NO: 55175
1-78
gi|1147729
2525-2448
98%

Seq ID NO: 55175
115-160
gi|1147729
2413-2368
97%

Seq ID NO: 55175
1-78
gi|2232021
87-10
98%

Seq ID NO: 55226
1-24
gi|33139696
554-577
100%

Seq ID NO: 55245
1-25
gi|32324959
40-64
96%

Seq ID NO: 55392
173-276
gi|35504550
57-160
99%

Seq ID NO: 55392
326-425
gi|35504550
164-263
97%

Seq ID NO: 55392
1-66
gi|35504862
10-74
89%

Seq ID NO: 55425
57-187
gi|54545475
93-224
92%

Seq ID NO: 55425
231-321
gi|54545475
220-310
90%

Seq ID NO: 55468
1-141
gi|32325191
449-589
94%

Seq ID NO: 55545
75-99
gi|32325459
266-290
100%

Seq ID NO: 55553
1-116
gi|33139587
121-6
94%

Seq ID NO: 55566
167-228
gi|18083080
182-243
88%

Seq ID NO: 55640
887-1044
gi|33139586
1-158
96%

Seq ID NO: 55660
21-138
gi|28916076
1-119
91%

Seq ID NO: 55660
14-41
gi|159473
34-61
100%

Seq ID NO: 55660
14-41
gi|2454547
227-200
100%

Seq ID NO: 55660
14-41
gi|551594
194-221
100%

Seq ID NO: 55660
14-41
gi|551595
669-696
100%

Seq ID NO: 55660
14-41
gi|18032254
34-61
100%

Seq ID NO: 55660
18-41
gi|18477256
279-256
100%

Seq ID NO: 55660
18-41
gi|18477260
325-302
100%

Seq ID NO: 55660
18-41
gi|18477262
634-611
100%

Seq ID NO: 55660
14-35
gi|18477259
22-1
100%

Seq ID NO: 55660
14-35
gi|18477261
22-1
100%

Seq ID NO: 55660
14-35
gi|37780968
22-1
100%

Seq ID NO: 55719
1-146
gi|18080802
354-209
97%

Seq ID NO: 55719
1-131
gi|7144157
137-7
97%

Seq ID NO: 55719
1-130
gi|18088100
130-1
97%

Seq ID NO: 55719
1-124
gi|54549688
124-1
96%

Seq ID NO: 55719
1-118
gi|18089409
118-1
97%

Seq ID NO: 55719
1-117
gi|18090782
117-1
97%

Seq ID NO: 55719
25-146
gi|52128973
94-215
88%

Seq ID NO: 55719
1-78
gi|18083086
537-614
96%

Seq ID NO: 55719
1-75
gi|18090403
75-1
96%

Seq ID NO: 55719
25-121
gi|52129301
319-415
86%

Seq ID NO: 55719
100-146
gi|52129005
1-47
95%

Seq ID NO: 55719
83-146
gi|9033884
441-503
90%

Seq ID NO: 55719
83-146
gi|15768080
331-393
90%

Seq ID NO: 55719
71-146
gi|19383550
397-471
88%

Seq ID NO: 55719
83-146
gi|39747546
344-406
90%

Seq ID NO: 55719
100-146
gi|39747568
266-312
95%

Seq ID NO: 55719
100-146
gi|46984679
145-99
95%

Seq ID NO: 55719
105-146
gi|15769768
391-432
97%

Seq ID NO: 55719
105-146
gi|40669510
255-296
97%

Seq ID NO: 55719
100-140
gi|46986671
175-135
97%

Seq ID NO: 55719
108-146
gi|31326272
1-39
97%

Seq ID NO: 55719
105-146
gi|159682
379-420
92%

Seq ID NO: 55759
302-322
gi|46984484
89-109
100%

Seq ID NO: 55808
1-74
gi|54545841
238-311
91%

Seq ID NO: 55827
140-161
gi|46983788
125-146
100%

Seq ID NO: 55849
95-269
gi|54548527
231-405
87%

Seq ID NO: 55849
138-269
gi|54545989
2-133
88%

Seq ID NO: 55931
140-268
gi|33139063
130-258
96%

Seq ID NO: 55931
1-60
gi|33139063
71-130
98%

Seq ID NO: 56068
123-187
gi|21432594
122-186
86%

Seq ID NO: 56211
1-101
gi|7144157
39-139
97%

Seq ID NO: 56211
1-101
gi|54549688
26-126
96%

Seq ID NO: 56211
22-101
gi|18083086
614-535
96%

Seq ID NO: 56211
25-101
gi|18090403
1-77
96%

Seq ID NO: 56211
54-75
gi|52129301
340-319
100%

Seq ID NO: 56333
36-56
gi|33140432
264-284
100%

Seq ID NO: 56402
639-718
gi|33139730
156-76
93%

Seq ID NO: 56411
1-73
gi|16797830
749-821
100%

Seq ID NO: 56411
1-73
gi|16797832
747-819
100%

Seq ID NO: 56411
1-73
gi|26000759
745-817
100%

Seq ID NO: 56411
1-73
gi|31442320
746-818
100%

Seq ID NO: 56411
1-73
gi|31442322
746-818
98%

Seq ID NO: 56411
8-73
gi|21885259
776-841
98%

Seq ID NO: 45947
974-995
gi|46988291
426-405
100%

Seq ID NO: 56551
187-242
gi|18381363
1139-1194
91%

Seq ID NO: 56640
1-65
gi|33140765
16-80
98%

Seq ID NO: 56699
36-174
gi|28916076
140-1
92%

Seq ID NO: 56699
154-181
gi|159473
61-34
100%

Seq ID NO: 56699
154-181
gi|2454547
200-227
100%

Seq ID NO: 56699
154-181
gi|551594
221-194
100%

Seq ID NO: 56699
154-181
gi|551595
696-669
100%

Seq ID NO: 56699
154-181
gi|18032254
61-34
100%

Seq ID NO: 56699
154-177
gi|18477256
256-279
100%

Seq ID NO: 56699
154-177
gi|18477260
302-325
100%

Seq ID NO: 56699
154-177
gi|18477262
611-634
100%

Seq ID NO: 56699
160-181
gi|18477259
1-22
100%

Seq ID NO: 56699
160-181
gi|18477261
1-22
100%

Seq ID NO: 56699
160-181
gi|37780968
1-22
100%

Seq ID NO: 56752
348-369
gi|7235459
41-20
100%

Seq ID NO: 45973
404-544
gi|54546305
483-623
88%

Seq ID NO: 45973
181-293
gi|54546305
345-457
83%

Seq ID NO: 45973
434-544
gi|54544886
269-379
90%

Seq ID NO: 56872
764-956
gi|33140201
149-341
99%

Seq ID NO: 56872
569-719
gi|33140201
1-151
98%

Seq ID NO: 56889
189-333
gi|54544976
245-389
89%

Seq ID NO: 56927
75-226
gi|33139131
99-250
87%

Seq ID NO: 56927
81-221
gi|32324211
1-141
87%

Seq ID NO: 56972
1-101
gi|35504916
448-548
96%

Seq ID NO: 56972
1-85
gi|35504725
448-533
94%

Seq ID NO: 57065
168-188
gi|33952655
9-29
100%

Seq ID NO: 57065
168-188
gi|46422144
284-304
100%

Seq ID NO: 57084
62-214
gi|33140673
201-49
92%

Seq ID NO: 57084
71-214
gi|33139639
245-388
93%

Seq ID NO: 57086
190-217
gi|54547991
157-184
96%

Seq ID NO: 57220
1-66
gi|37517244
1816-1881
96%

Seq ID NO: 57351
5-196
gi|32323971
353-544
98%

Seq ID NO: 57416
412-434
gi|551595
853-875
100%

Seq ID NO: 57422
783-808
gi|46986223
315-290
100%

Seq ID NO: 57468
1-221
gi|2239108
36-256
100%

Seq ID NO: 57539
18-223
gi|32325381
184-389
92%

Seq ID NO: 57539
415-498
gi|32325381
398-481
97%

Seq ID NO: 57539
174-223
gi|33139814
8-57
100%

Seq ID NO: 57713
253-552
gi|33139068
28-327
97%

Seq ID NO: 57713
633-701
gi|33139068
334-402
98%

Seq ID NO: 57713
169-198
gi|33139068
1-30
100%

Seq ID NO: 57713
750-877
gi|33139557
441-568
93%

Seq ID NO: 57713
176-198
gi|33139557
1-23
100%

Seq ID NO: 57713
750-892
gi|33140099
424-566
92%

Seq ID NO: 57713
255-552
gi|33139079
16-313
97%

Seq ID NO: 57713
750-849
gi|33139079
434-533
94%

Seq ID NO: 57713
1-198
gi|32324666
26-223
96%

Seq ID NO: 57713
633-661
gi|32324666
527-555
100%

Seq ID NO: 57713
271-552
gi|33139103
1-282
97%

Seq ID NO: 57713
287-554
gi|33139308
38-306
97%

Seq ID NO: 57713
637-864
gi|33139308
315-545
90%

Seq ID NO: 57713
1232-1423
gi|33139521
162-353
96%

Seq ID NO: 57713
811-966
gi|33139521
1-159
89%

Seq ID NO: 57713
750-878
gi|33140185
112-240
93%

Seq ID NO: 57713
636-701
gi|33140185
1-66
98%

Seq ID NO: 57713
300-551
gi|32325329
51-302
90%

Seq ID NO: 57713
1229-1384
gi|32325562
408-563
99%

Seq ID NO: 57713
750-966
gi|32325562
189-408
89%

Seq ID NO: 57713
598-701
gi|32325562
40-143
96%

Seq ID NO: 57713
300-534
gi|32324015
367-601
90%

Seq ID NO: 57713
1229-1374
gi|33140373
408-553
100%

Seq ID NO: 57713
655-698
gi|32325327
481-524
90%

Seq ID NO: 57713
655-776
gi|32325443
481-599
83%

Seq ID NO: 57713
300-533
gi|33140304
225-458
89%

Seq ID NO: 57713
300-517
gi|33140764
367-584
89%

Seq ID NO: 57713
1229-1347
gi|32324619
408-526
100%

Seq ID NO: 57713
300-490
gi|32325078
367-557
90%

Seq ID NO: 57713
1254-1423
gi|33139825
258-427
90%

Seq ID NO: 57713
300-453
gi|33139892
410-563
90%

Seq ID NO: 57713
300-452
gi|33140637
410-562
90%

Seq ID NO: 57713
1288-1423
gi|32324536
247-382
91%

Seq ID NO: 57713
414-551
gi|32324536
10-147
89%

Seq ID NO: 57713
384-551
gi|33140589
1-168
88%

Seq ID NO: 57713
393-551
gi|33140074
4-162
88%

Seq ID NO: 57713
655-896
gi|32325371
173-411
83%

Seq ID NO: 57713
300-401
gi|32324162
476-577
92%

Seq ID NO: 57713
428-551
gi|32324623
225-348
88%

Seq ID NO: 57713
300-375
gi|32324623
103-178
93%

Seq ID NO: 57713
300-353
gi|33139035
491-544
94%

Seq ID NO: 57793
1-189
gi|33139897
347-535
91%

Seq ID NO: 57824
6-146
gi|32324211
1-141
88%

Seq ID NO: 57824
17-151
gi|33139131
116-250
88%

Seq ID NO: 57824
1-145
gi|33140673
193-49
83%

Seq ID NO: 57824
13-145
gi|33139639
256-388
82%

Seq ID NO: 57824
152-172
gi|45563795
521-501
100%

Seq ID NO: 57907
1213-1367
gi|18081696
127-281
85%

Seq ID NO: 57996
664-684
gi|39747573
93-113
100%

Seq ID NO: 58001
4-65
gi|27541441
265-326
84%

Seq ID NO: 46037
415-488
gi|21393234
329-402
91%

Seq ID NO: 46037
117-143
gi|21393234
148-174
100%

Seq ID NO: 46037
377-488
gi|18080246
261-372
85%

Seq ID NO: 46037
418-488
gi|18381808
299-229
90%

Seq ID NO: 46037
427-488
gi|52128281
271-332
91%

Seq ID NO: 58171
85-106
gi|22139607
133-112
100%

Seq ID NO: 58223
117-138
gi|16746280
49-70
100%

Seq ID NO: 58237
123-269
gi|33139639
245-388
89%

Seq ID NO: 58237
114-269
gi|33140673
201-49
87%

Seq ID NO: 58237
121-246
gi|33139131
99-224
82%

Seq ID NO: 58237
333-378
gi|32324211
211-256
91%

Seq ID NO: 58264
869-1010
gi|18082514
100-241
90%

Seq ID NO: 58264
1203-1298
gi|18082514
245-340
92%

Seq ID NO: 58357
136-238
gi|54549492
497-599
88%

Seq ID NO: 58357
369-416
gi|54549492
633-680
91%

Seq ID NO: 58382
12-159
gi|18383012
1144-1291
84%

Seq ID NO: 58427
1-143
gi|16797830
62-204
97%

Seq ID NO: 58427
1-143
gi|16797831
62-202
97%

Seq ID NO: 58427
1-143
gi|16797832
63-203
97%

Seq ID NO: 58427
1-143
gi|47118285
74-214
96%

Seq ID NO: 58427
1-142
gi|26000762
62-201
95%

Seq ID NO: 58427
1-143
gi|31442322
62-202
95%

Seq ID NO: 58427
1-143
gi|38096133
62-202
93%

Seq ID NO: 58438
319-443
gi|32324393
180-307
96%

Seq ID NO: 58438
1-83
gi|32324393
25-108
98%

Seq ID NO: 58438
154-195
gi|32324393
107-148
100%

Seq ID NO: 58438
239-273
gi|32324393
147-181
100%

Seq ID NO: 58438
319-383
gi|33140257
413-477
98%

Seq ID NO: 58438
154-191
gi|33140257
345-382
100%

Seq ID NO: 58478
192-238
gi|18089268
22-68
89%

Seq ID NO: 58670
81-148
gi|18090251
452-519
88%

Seq ID NO: 58722
346-432
gi|19266104
21-107
84%

Seq ID NO: 46083
1-119
gi|33139765
458-576
99%

Seq ID NO: 46083
265-305
gi|52128426
396-436
95%

Seq ID NO: 46084
295-415
gi|33139765
576-456
99%

Seq ID NO: 46084
479-528
gi|33139765
454-405
100%

Seq ID NO: 46084
109-149
gi|52128426
436-396
95%

Seq ID NO: 58765
150-170
gi|18081306
417-437
100%

Seq ID NO: 58768
2-66
gi|7143917
219-283
90%

Seq ID NO: 58912
483-676
gi|7144233
308-501
87%

Seq ID NO: 58912
483-667
gi|7144339
311-495
86%

Seq ID NO: 58913
201-271
gi|52128794
102-172
90%

Seq ID NO: 58913
187-272
gi|46985091
319-234
85%

Seq ID NO: 58913
85-121
gi|18080422
365-401
94%

Seq ID NO: 58913
249-272
gi|46985022
259-236
100%

Seq ID NO: 59048
23-135
gi|33139660
491-603
98%

Seq ID NO: 59083
221-245
gi|46987193
49-25
96%

Seq ID NO: 59131
1-107
gi|18082168
3-109
94%

Seq ID NO: 59131
16-108
gi|51334509
37-129
88%

Seq ID NO: 59131
70-108
gi|52129549
138-176
89%

Seq ID NO: 59132
1-56
gi|7641359
475-420
91%

Seq ID NO: 59132
1-59
gi|14085900
55-113
90%

Seq ID NO: 59132
1-47
gi|14086110
51-97
93%

Seq ID NO: 59132
1-56
gi|15767660
65-120
89%

Seq ID NO: 59132
1-56
gi|18495580
501-446
89%

Seq ID NO: 59132
19-59
gi|39747527
61-101
92%

Seq ID NO: 59201
21-138
gi|28916076
1-119
91%

Seq ID NO: 59201
14-41
gi|159473
34-61
100%

Seq ID NO: 59201
14-41
gi|2454547
227-200
100%

Seq ID NO: 59201
14-41
gi|551594
194-221
100%

Seq ID NO: 59201
14-41
gi|551595
669-696
100%

Seq ID NO: 59201
14-41
gi|18032254
34-61
100%

Seq ID NO: 59201
18-41
gi|18477256
279-256
100%

Seq ID NO: 59201
18-41
gi|18477260
325-302
100%

Seq ID NO: 59201
18-41
gi|18477262
634-611
100%

Seq ID NO: 59201
14-35
gi|18477259
22-1
100%

Seq ID NO: 59201
14-35
gi|18477261
22-1
100%

Seq ID NO: 59201
14-35
gi|37780968
22-1
100%

Seq ID NO: 59268
131-256
gi|33139189
152-277
99%

Seq ID NO: 59268
1-56
gi|33139189
96-151
98%

Seq ID NO: 59269
3-85
gi|7143543
482-564
88%

Seq ID NO: 59311
1-81
gi|7144052
441-521
90%

Seq ID NO: 59484
1-118
gi|18080802
329-209
95%

Seq ID NO: 59484
1-103
gi|7144157
112-7
95%

Seq ID NO: 59484
1-102
gi|18088100
105-1
95%

Seq ID NO: 59484
1-96
gi|54549688
99-1
93%

Seq ID NO: 59484
1-90
gi|18089409
93-1
94%

Seq ID NO: 59484
1-89
gi|18090782
92-1
94%

Seq ID NO: 59484
56-118
gi|9033884
441-503
90%

Seq ID NO: 59484
56-118
gi|15768080
331-393
90%

Seq ID NO: 59484
56-118
gi|39747546
344-406
90%

Seq ID NO: 59484
77-118
gi|15769768
391-432
97%

Seq ID NO: 59484
77-118
gi|19383550
430-471
97%

Seq ID NO: 59484
77-118
gi|39747568
271-312
97%

Seq ID NO: 59484
77-112
gi|46986671
170-135
100%

Seq ID NO: 59484
80-118
gi|31326272
1-39
97%

Seq ID NO: 59484
77-118
gi|159682
379-420
92%

Seq ID NO: 59484
77-118
gi|15784370
177-218
92%

Seq ID NO: 59530
1-129
gi|33139697
192-63
97%

Seq ID NO: 59558
15-54
gi|28916076
1-40
97%

Seq ID NO: 59558
8-35
gi|159473
34-61
100%

Seq ID NO: 59558
8-35
gi|2454547
227-200
100%

Seq ID NO: 59558
8-35
gi|551594
194-221
100%

Seq ID NO: 59558
8-35
gi|551595
669-696
100%

Seq ID NO: 59558
8-35
gi|18032254
34-61
100%

Seq ID NO: 59558
12-35
gi|18477256
279-256
100%

Seq ID NO: 59558
12-35
gi|18477260
325-302
100%

Seq ID NO: 59558
12-35
gi|18477262
634-611
100%

Seq ID NO: 59558
8-29
gi|18477259
22-1
100%

Seq ID NO: 59558
8-29
gi|18477261
22-1
100%

Seq ID NO: 59558
8-29
gi|37780968
22-1
100%

Seq ID NO: 59597
141-230
gi|33139639
297-386
85%

Seq ID NO: 59597
141-193
gi|33140673
140-88
90%

Seq ID NO: 59652
7-89
gi|33140014
182-264
100%

Seq ID NO: 59901
94-114
gi|7798143
245-225
100%

Seq ID NO: 59911
1-141
gi|2239106
1166-1307
98%

Seq ID NO: 59911
1-141
gi|37517243
1166-1307
98%

Seq ID NO: 59944
178-340
gi|33139639
257-419
89%

Seq ID NO: 59944
159-310
gi|33140673
199-48
87%

Seq ID NO: 60099
81-221
gi|32324211
1-141
90%

Seq ID NO: 60099
92-226
gi|33139131
116-250
89%

Seq ID NO: 60099
305-349
gi|33139131
293-337
93%

Seq ID NO: 60099
70-220
gi|33140673
199-49
86%

Seq ID NO: 60099
89-264
gi|33139639
257-429
84%

Seq ID NO: 60155
1-67
gi|7144157
200-134
91%

Seq ID NO: 60155
1-67
gi|54549688
187-121
91%

Seq ID NO: 60206
46-72
gi|159473
61-35
100%

Seq ID NO: 60206
46-72
gi|2454547
200-226
100%

Seq ID NO: 60206
46-72
gi|551594
221-195
100%

Seq ID NO: 60206
46-72
gi|551595
696-670
100%

Seq ID NO: 60206
46-72
gi|18032254
61-35
100%

Seq ID NO: 60206
46-69
gi|18477256
256-279
100%

Seq ID NO: 60206
46-69
gi|18477260
302-325
100%

Seq ID NO: 60206
46-69
gi|18477262
611-634
100%

Seq ID NO: 60206
52-72
gi|18477259
1-21
100%

Seq ID NO: 60206
52-72
gi|18477261
1-21
100%

Seq ID NO: 60206
52-72
gi|37780968
1-21
100%

Seq ID NO: 60211
305-361
gi|18081798
206-262
91%

Seq ID NO: 60211
305-361
gi|54547765
204-260
89%

Seq ID NO: 60211
52-77
gi|9829211
55-80
100%

Seq ID NO: 60211
52-77
gi|21652727
26-51
100%

Seq ID NO: 60220
1-95
gi|33140348
161-255
96%

Seq ID NO: 60378
15-74
gi|28916076
1-60
93%

Seq ID NO: 60378
8-35
gi|159473
34-61
100%

Seq ID NO: 60378
8-35
gi|2454547
227-200
100%

Seq ID NO: 60378
8-35
gi|551594
194-221
100%

Seq ID NO: 60378
8-35
gi|551595
669-696
100%

Seq ID NO: 60378
8-35
gi|18032254
34-61
100%

Seq ID NO: 60378
12-35
gi|18477256
279-256
100%

Seq ID NO: 60378
12-35
gi|18477260
325-302
100%

Seq ID NO: 60378
12-35
gi|18477262
634-611
100%

Seq ID NO: 60378
8-29
gi|18477259
22-1
100%

Seq ID NO: 60378
8-29
gi|18477261
22-1
100%

Seq ID NO: 60378
8-29
gi|37780968
22-1
100%

Seq ID NO: 60409
273-620
gi|32324347
2-350
96%

Seq ID NO: 60409
680-806
gi|32324347
351-477
98%

Seq ID NO: 60409
680-784
gi|33139657
351-455
98%

Seq ID NO: 60409
289-615
gi|33140241
13-338
97%

Seq ID NO: 60409
677-806
gi|33140241
341-470
98%

Seq ID NO: 60409
272-619
gi|33139259
1-348
95%

Seq ID NO: 60409
278-620
gi|33139000
12-355
96%

Seq ID NO: 60409
272-615
gi|33139478
1-339
96%

Seq ID NO: 60409
268-620
gi|32324919
1-354
95%

Seq ID NO: 60409
273-609
gi|33139390
2-339
95%

Seq ID NO: 60409
391-620
gi|33140350
66-296
96%

Seq ID NO: 60409
273-337
gi|33140350
2-66
93%

Seq ID NO: 60409
498-615
gi|33139683
1-118
98%

Seq ID NO: 60495
1-88
gi|5107411
569-656
100%

Seq ID NO: 60495
1-88
gi|16797830
379-466
100%

Seq ID NO: 60495
1-88
gi|16797831
377-464
98%

Seq ID NO: 60495
1-88
gi|16797832
378-465
98%

Seq ID NO: 60495
1-88
gi|31442322
377-464
98%

Seq ID NO: 60495
5-88
gi|26000760
381-464
98%

Seq ID NO: 60495
1-88
gi|38096135
377-465
95%

Seq ID NO: 60495
12-86
gi|31442314
392-467
93%

Seq ID NO: 60495
4-86
gi|38096138
386-468
90%

Seq ID NO: 60495
3-86
gi|16797838
399-482
89%

Seq ID NO: 60532
243-263
gi|46986928
44-64
100%

Seq ID NO: 60533
237-257
gi|46986928
64-44
100%

Seq ID NO: 60534
122-248
gi|33139568
363-489
99%

Seq ID NO: 60534
1-75
gi|33139568
293-367
98%

Seq ID NO: 60596
1-99
gi|33139131
122-220
89%

Seq ID NO: 60596
29-92
gi|33140673
143-80
87%

Seq ID NO: 60782
367-405
gi|54547981
61-24
94%

Seq ID NO: 60782
792-812
gi|19435901
24-4
100%

Seq ID NO: 60783
218-256
gi|54547981
24-61
94%

Seq ID NO: 60788
36-165
gi|28916076
140-10
92%

Seq ID NO: 60644
26-217
gi|18080216
20-211
86%

Seq ID NO: 60947
1-85
gi|33140348
171-255
97%

Seq ID NO: 61051
73-95
gi|39746742
647-625
100%

Seq ID NO: 61179
139-160
gi|34316675
229-208
100%

Seq ID NO: 61217
76-324
gi|33139639
245-493
87%

Seq ID NO: 61217
71-219
gi|33140673
197-49
89%

Seq ID NO: 61217
115-219
gi|32324211
36-140
88%

Seq ID NO: 61303
36-174
gi|28916076
140-1
92%

Seq ID NO: 61303
154-181
gi|159473
61-34
100%

Seq ID NO: 61303
154-181
gi|2454547
200-227
100%

Seq ID NO: 61303
154-181
gi|551594
221-194
100%

Seq ID NO: 61303
154-181
gi|551595
696-669
100%

Seq ID NO: 61303
154-181
gi|18032254
61-34
100%

Seq ID NO: 61303
154-177
gi|18477256
256-279
100%

Seq ID NO: 61303
154-177
gi|18477260
302-325
100%

Seq ID NO: 61303
154-177
gi|18477262
611-634
100%

Seq ID NO: 61303
160-181
gi|18477259
1-22
100%

Seq ID NO: 61303
160-181
gi|18477261
1-22
100%

Seq ID NO: 61303
160-181
gi|37780968
1-22
100%

Seq ID NO: 61351
4-140
gi|18080057
229-93
85%

Seq ID NO: 61351
4-140
gi|54547707
233-97
84%

Seq ID NO: 61352
186-312
gi|18080057
303-429
90%

Seq ID NO: 61352
1-134
gi|18080057
159-292
88%

Seq ID NO: 61352
1-139
gi|54547707
163-300
88%

Seq ID NO: 61352
186-312
gi|54547707
307-433
89%

Seq ID NO: 61352
363-405
gi|54547707
443-485
90%

Seq ID NO: 61526
29-138
gi|28916076
9-119
91%

Seq ID NO: 61553
31-90
gi|51237572
280-339
86%

Seq ID NO: 46246
1-165
gi|33139608
404-567
98%

Seq ID NO: 46246
1-62
gi|32323997
404-464
98%

Seq ID NO: 61561
480-500
gi|54546754
60-40
100%

Seq ID NO: 61600
3-49
gi|32324211
94-140
93%

Seq ID NO: 61643
680-700
gi|23260226
280-260
100%

Seq ID NO: 61686
1-73
gi|16797830
749-821
100%

Seq ID NO: 61686
1-73
gi|16797832
747-819
100%

Seq ID NO: 61686
1-73
gi|26000759
745-817
100%

Seq ID NO: 61686
1-73
gi|31442320
746-818
100%

Seq ID NO: 61686
1-73
gi|31442322
746-818
98%

Seq ID NO: 61686
8-73
gi|21885259
776-841
98%

Seq ID NO: 61688
97-117
gi|52128725
61-81
100%

Seq ID NO: 61719
701-721
gi|32185118
164-144
100%

Seq ID NO: 61810
13-290
gi|33140136
39-317
85%

Seq ID NO: 61937
5-25
gi|19267937
180-160
100%

Seq ID NO: 61955
1-89
gi|33139270
471-383
100%

Seq ID NO: 61980
1-78
gi|33140673
126-49
92%

Seq ID NO: 61980
19-84
gi|33139131
185-250
92%

Seq ID NO: 61980
250-309
gi|33139639
521-580
91%

Seq ID NO: 46268
777-960
gi|33140770
55-237
99%

Seq ID NO: 46268
639-695
gi|33140770
1-57
98%

Seq ID NO: 62170
207-227
gi|46422740
369-349
100%

Seq ID NO: 62176
200-225
gi|30028086
358-383
100%

Seq ID NO: 62176
198-218
gi|16005270
477-497
100%

Seq ID NO: 46279
5-190
gi|35504456
365-180
96%

Seq ID NO: 46279
63-188
gi|7143616
133-258
91%

Seq ID NO: 46279
63-188
gi|18381740
382-507
91%

Seq ID NO: 46279
63-184
gi|18381740
131-252
91%

Seq ID NO: 46279
76-188
gi|18381923
603-491
92%

Seq ID NO: 46279
73-188
gi|21493480
425-310
85%

Seq ID NO: 62414
1-128
gi|32323987
182-309
100%

Seq ID NO: 62414
173-246
gi|32323987
309-382
100%

Seq ID NO: 62414
173-222
gi|33140527
311-360
98%

Seq ID NO: 62539
200-225
gi|54547695
79-104
96%

Seq ID NO: 62576
170-298
gi|33139556
85-213
94%

Seq ID NO: 62576
42-126
gi|33139556
1-85
98%

Seq ID NO: 46293
154-303
gi|18080373
337-486
91%

Seq ID NO: 62611
1-45
gi|33139645
197-241
97%

Seq ID NO: 62636
254-285
gi|18382597
630-661
96%

Seq ID NO: 46302
262-359
gi|18381117
806-709
92%

Seq ID NO: 46302
262-362
gi|7144103
148-248
91%

Seq ID NO: 46305
314-411
gi|54548221
329-232
88%

Seq ID NO: 62776
3-93
gi|33140348
248-158
89%

Seq ID NO: 62899
1-140
gi|32325217
127-267
96%

Seq ID NO: 62904
154-306
gi|18082113
218-370
89%

Seq ID NO: 62904
158-306
gi|54549200
528-380
87%

Seq ID NO: 62904
159-306
gi|54545591
457-310
87%

Seq ID NO: 62904
179-306
gi|7144024
503-377
87%

Seq ID NO: 62986
504-558
gi|32324842
158-212
92%

Seq ID NO: 63015
1-98
gi|32324253
114-17
94%

Seq ID NO: 63100
554-708
gi|32324459
81-235
95%

Seq ID NO: 63107
36-174
gi|28916076
140-1
92%

Seq ID NO: 63107
154-181
gi|159473
61-34
100%

Seq ID NO: 63107
154-181
gi|2454547
200-227
100%

Seq ID NO: 63107
154-181
gi|551594
221-194
100%

Seq ID NO: 63107
154-181
gi|551595
696-669
100%

Seq ID NO: 63107
154-181
gi|18032254
61-34
100%

Seq ID NO: 63107
154-177
gi|18477256
256-279
100%

Seq ID NO: 63107
154-177
gi|18477260
302-325
100%

Seq ID NO: 63107
154-177
gi|18477262
611-634
100%

Seq ID NO: 63107
160-181
gi|18477259
1-22
100%

Seq ID NO: 63107
160-181
gi|18477261
1-22
100%

Seq ID NO: 63107
160-181
gi|37780968
1-22
100%

Seq ID NO: 63189
13-47
gi|7143962
302-269
94%

Seq ID NO: 63384
576-772
gi|32325427
297-493
97%

Seq ID NO: 63384
217-381
gi|32325427
50-214
98%

Seq ID NO: 63384
428-514
gi|32325427
213-299
100%

Seq ID NO: 63384
110-159
gi|32325427
1-50
94%

Seq ID NO: 63384
629-772
gi|18089917
1-144
88%

Seq ID NO: 63450
15-54
gi|28916076
1-40
97%

Seq ID NO: 63450
8-35
gi|159473
34-61
100%

Seq ID NO: 63450
8-35
gi|2454547
227-200
100%

Seq ID NO: 63450
8-35
gi|551594
194-221
100%

Seq ID NO: 63450
8-35
gi|551595
669-696
100%

Seq ID NO: 63450
8-35
gi|18032254
34-61
100%

Seq ID NO: 63450
12-35
gi|18477256
279-256
100%

Seq ID NO: 63450
12-35
gi|18477260
325-302
100%

Seq ID NO: 63450
12-35
gi|18477262
634-611
100%

Seq ID NO: 63450
8-29
gi|18477259
22-1
100%

Seq ID NO: 63450
8-29
gi|18477261
22-1
100%

Seq ID NO: 63450
8-29
gi|37780968
22-1
100%

Seq ID NO: 63508
16-41
gi|30166029
39-64
96%

Seq ID NO: 63529
4-111
gi|33140348
148-255
93%

Seq ID NO: 46344
3-176
gi|54548367
26-199
85%

Seq ID NO: 46344
461-533
gi|54548367
299-371
90%

Seq ID NO: 63626
1-138
gi|18090776
20-157
88%

Seq ID NO: 46358
286-423
gi|7144085
453-591
91%

Seq ID NO: 63905
1-73
gi|16797830
749-821
100%

Seq ID NO: 63905
1-73
gi|16797832
747-819
100%

Seq ID NO: 63905
1-73
gi|26000759
745-817
100%

Seq ID NO: 63905
1-73
gi|31442320
746-818
100%

Seq ID NO: 63905
1-73
gi|31442322
746-818
98%

Seq ID NO: 63905
8-73
gi|21885259
776-841
98%

Seq ID NO: 63930
36-103
gi|18080500
393-460
86%

Seq ID NO: 64030
21-138
gi|28916076
1-119
91%

Seq ID NO: 64030
14-41
gi|159473
34-61
100%

Seq ID NO: 64030
14-41
gi|2454547
227-200
100%

Seq ID NO: 64030
14-41
gi|551594
194-221
100%

Seq ID NO: 64030
14-41
gi|551595
669-696
100%

Seq ID NO: 64030
14-41
gi|18032254
34-61
100%

Seq ID NO: 64030
18-41
gi|18477256
279-256
100%

Seq ID NO: 64030
18-41
gi|18477260
325-302
100%

Seq ID NO: 64030
18-41
gi|18477262
634-611
100%

Seq ID NO: 64030
14-35
gi|18477259
22-1
100%

Seq ID NO: 64030
14-35
gi|18477261
22-1
100%

Seq ID NO: 64030
14-35
gi|37780968
22-1
100%

Seq ID NO: 64057
318-354
gi|33139730
82-119
97%

Seq ID NO: 64082
276-364
gi|32325034
20-108
96%

Seq ID NO: 64082
74-140
gi|32324889
134-68
100%

Seq ID NO: 64082
325-364
gi|33139589
1-40
97%

Seq ID NO: 64082
74-147
gi|21393259
27-100
85%

Seq ID NO: 64082
74-147
gi|20498904
25-98
85%

Seq ID NO: 64106
2-35
gi|20064584
1-34
97%

Seq ID NO: 64146
339-442
gi|33140237
332-229
100%

Seq ID NO: 64146
21-109
gi|33140237
419-331
97%

Seq ID NO: 64146
452-518
gi|33140237
219-153
98%

Seq ID NO: 64146
580-617
gi|33140237
151-114
100%

Seq ID NO: 64148
457-691
gi|32325211
200-434
98%

Seq ID NO: 64148
302-406
gi|32325211
96-200
96%

Seq ID NO: 64148
145-221
gi|32325211
24-99
96%

Seq ID NO: 64148
735-788
gi|32325211
434-487
100%

Seq ID NO: 64148
897-948
gi|32325211
540-591
100%

Seq ID NO: 64148
798-842
gi|32325211
497-541
100%

Seq ID NO: 64148
75-101
gi|32325211
1-27
100%

Seq ID NO: 64148
897-1003
gi|32324168
477-583
99%

Seq ID NO: 64148
186-221
gi|32324168
1-36
97%

Seq ID NO: 64148
457-684
gi|33139272
319-546
98%

Seq ID NO: 64148
1-101
gi|33139272
46-146
99%

Seq ID NO: 64148
897-996
gi|33139975
485-584
99%

Seq ID NO: 64148
178-221
gi|33139975
1-44
97%

Seq ID NO: 64181
21-138
gi|28916076
1-119
91%

Seq ID NO: 64181
14-41
gi|159473
34-61
100%

Seq ID NO: 64181
14-41
gi|2454547
227-200
100%

Seq ID NO: 64181
14-41
gi|551594
194-221
100%

Seq ID NO: 64181
14-41
gi|551595
669-696
100%

Seq ID NO: 64181
14-41
gi|18032254
34-61
100%

Seq ID NO: 64181
18-41
gi|18477256
279-256
100%

Seq ID NO: 64181
18-41
gi|18477260
325-302
100%

Seq ID NO: 64181
18-41
gi|18477262
634-611
100%

Seq ID NO: 64181
14-35
gi|18477259
22-1
100%

Seq ID NO: 64181
14-35
gi|18477261
22-1
100%

Seq ID NO: 64181
14-35
gi|37780968
22-1
100%

Seq ID NO: 64254
169-189
gi|18083118
116-136
100%

Seq ID NO: 64254
169-189
gi|18382418
749-769
100%

Seq ID NO: 64268
399-518
gi|54546846
257-382
82%

Seq ID NO: 64305
240-260
gi|24467850
41-21
100%

Seq ID NO: 64309
148-249
gi|18088868
401-300
90%

Seq ID NO: 46394
286-347
gi|46987704
361-300
85%

Seq ID NO: 64348
295-324
gi|7922575
190-218
96%

Seq ID NO: 64377
645-702
gi|54544818
520-577
91%

Seq ID NO: 64390
83-126
gi|551594
1028-1070
93%

Seq ID NO: 64390
83-126
gi|551595
267-309
93%

Seq ID NO: 64477
21-138
gi|28916076
1-119
91%

Seq ID NO: 64477
14-41
gi|159473
34-61
100%

Seq ID NO: 64477
14-41
gi|2454547
227-200
100%

Seq ID NO: 64477
14-41
gi|551594
194-221
100%

Seq ID NO: 64477
14-41
gi|551595
669-696
100%

Seq ID NO: 64477
14-41
gi|18032254
34-61
100%

Seq ID NO: 64477
18-41
gi|18477256
279-256
100%

Seq ID NO: 64477
18-41
gi|18477260
325-302
100%

Seq ID NO: 64477
18-41
gi|18477262
634-611
100%

Seq ID NO: 64477
14-35
gi|18477259
22-1
100%

Seq ID NO: 64477
14-35
gi|18477261
22-1
100%

Seq ID NO: 64477
14-35
gi|37780968
22-1
100%

Seq ID NO: 64508
27-157
gi|32324692
9-139
97%

Seq ID NO: 64514
52-204
gi|32324211
369-521
85%

Seq ID NO: 64570
21-138
gi|28916076
1-119
91%

Seq ID NO: 64570
14-41
gi|159473
34-61
100%

Seq ID NO: 64570
14-41
gi|2454547
227-200
100%

Seq ID NO: 64570
14-41
gi|551594
194-221
100%

Seq ID NO: 64570
14-41
gi|551595
669-696
100%

Seq ID NO: 64570
14-41
gi|18032254
34-61
100%

Seq ID NO: 64570
18-41
gi|18477256
279-256
100%

Seq ID NO: 64570
18-41
gi|18477260
325-302
100%

Seq ID NO: 64570
18-41
gi|18477262
634-611
100%

Seq ID NO: 64570
14-35
gi|18477259
22-1
100%

Seq ID NO: 64570
14-35
gi|18477261
22-1
100%

Seq ID NO: 64570
14-35
gi|37780968
22-1
100%

Seq ID NO: 64580
33-147
gi|32325191
1-115
92%

Seq ID NO: 64633
2-114
gi|33140530
1-113
100%

Seq ID NO: 64669
300-320
gi|52129537
617-637
100%

Seq ID NO: 64692
1-57
gi|30028941
55-111
98%

Seq ID NO: 64692
1-57
gi|33140204
55-111
98%

Seq ID NO: 64694
95-147
gi|30028941
402-454
100%

Seq ID NO: 64694
1-43
gi|30028941
308-350
100%

Seq ID NO: 64694
95-146
gi|30029072
395-446
100%

Seq ID NO: 64694
95-146
gi|33140204
402-453
100%

Seq ID NO: 64694
1-43
gi|33140204
308-350
100%

Seq ID NO: 64711
1-93
gi|33139524
233-141
98%

Seq ID NO: 64760
19-144
gi|54546846
257-383
84%

Seq ID NO: 64762
174-194
gi|9829276
404-424
100%

Seq ID NO: 64762
174-194
gi|39747004
520-540
100%

Seq ID NO: 64780
1-118
gi|33140548
49-166
95%

Seq ID NO: 64780
151-225
gi|33140548
199-273
100%

Seq ID NO: 64797
33-77
gi|54549640
11-55
93%

Seq ID NO: 64847
42-190
gi|33140348
108-255
92%

Seq ID NO: 64847
1-70
gi|33140348
105-174
92%

Seq ID NO: 64847
1-28
gi|32324498
17-44
96%

Seq ID NO: 64862
7-144
gi|7144222
169-306
92%

Seq ID NO: 64862
7-144
gi|54547280
139-276
92%

Seq ID NO: 64862
32-149
gi|51334182
168-285
88%

Seq ID NO: 64862
27-146
gi|21494008
204-323
86%

Seq ID NO: 64874
205-293
gi|18082074
129-217
83%

Seq ID NO: 64931
21-41
gi|46984838
273-293
100%

Seq ID NO: 64935
1-84
gi|16797830
749-832
98%

Seq ID NO: 64935
1-84
gi|16797832
747-830
98%

Seq ID NO: 64935
1-84
gi|26000759
745-828
98%

Seq ID NO: 64935
1-84
gi|31442320
746-829
98%

Seq ID NO: 64935
1-84
gi|31442322
746-829
97%

Seq ID NO: 64935
1-77
gi|16797831
747-823
98%

Seq ID NO: 64935
8-84
gi|21885259
776-852
97%

Seq ID NO: 65078
15-54
gi|28916076
1-40
97%

Seq ID NO: 65084
1-166
gi|35504916
448-612
94%

Seq ID NO: 65084
1-85
gi|35504725
448-533
94%

Seq ID NO: 65205
338-610
gi|33140653
131-403
100%

Seq ID NO: 65205
160-291
gi|33140653
1-132
97%

Seq ID NO: 65205
663-717
gi|33140653
401-455
100%

Seq ID NO: 65205
725-746
gi|33140653
463-484
100%

Seq ID NO: 65205
338-462
gi|32325169
130-254
100%

Seq ID NO: 65205
161-291
gi|32325169
1-131
97%

Seq ID NO: 65205
500-610
gi|32325169
254-364
100%

Seq ID NO: 65205
162-291
gi|33139964
1-130
98%

Seq ID NO: 65261
1-243
gi|33140806
9-255
95%

Seq ID NO: 65261
62-234
gi|33139704
24-199
95%

Seq ID NO: 46445
556-665
gi|33139443
1-111
95%

Seq ID NO: 65328
1-145
gi|18090481
97-241
85%

Seq ID NO: 46452
1-163
gi|7143710
57-220
89%

Seq ID NO: 46452
1-171
gi|54549870
42-213
89%

Seq ID NO: 46452
16-163
gi|18089601
5-153
90%

Seq ID NO: 46452
29-163
gi|54547929
1-136
90%

Seq ID NO: 46452
1-171
gi|51237630
23-194
85%

Seq ID NO: 65377
274-524
gi|33140174
121-371
98%

Seq ID NO: 65377
109-229
gi|33140174
1-121
99%

Seq ID NO: 65377
578-680
gi|33140174
372-474
100%

Seq ID NO: 65377
274-527
gi|32324003
302-555
98%

Seq ID NO: 65377
1-229
gi|32324003
74-302
97%

Seq ID NO: 65377
579-621
gi|32324003
554-596
97%

Seq ID NO: 65377
42-229
gi|32324933
13-200
97%

Seq ID NO: 65377
579-680
gi|32324933
452-553
99%

Seq ID NO: 65397
90-152
gi|18081066
414-476
90%

Seq ID NO: 65474
2-53
gi|28916076
52-1
94%

Seq ID NO: 65474
33-59
gi|159473
61-35
100%

Seq ID NO: 65474
33-59
gi|2454547
200-226
100%

Seq ID NO: 65474
33-59
gi|551594
221-195
100%

Seq ID NO: 65474
33-59
gi|551595
696-670
100%

Seq ID NO: 65474
33-59
gi|18032254
61-35
100%

Seq ID NO: 65474
33-56
gi|18477256
256-279
100%

Seq ID NO: 65474
33-56
gi|18477260
302-325
100%

Seq ID NO: 65474
33-56
gi|18477262
611-634
100%

Seq ID NO: 65474
39-59
gi|18477259
1-21
100%

Seq ID NO: 65474
39-59
gi|18477261
1-21
100%

Seq ID NO: 65474
39-59
gi|37780968
1-21
100%

Seq ID NO: 65553
1-63
gi|33140188
109-171
98%

Seq ID NO: 65553
2-50
gi|33140188
305-353
90%

Seq ID NO: 65558
14-173
gi|18383267
801-960
86%

Seq ID NO: 65560
480-623
gi|35504861
212-70
97%

Seq ID NO: 65572
411-582
gi|33139585
153-324
94%

Seq ID NO: 65572
135-258
gi|33139585
1-124
96%

Seq ID NO: 65591
217-309
gi|32324909
25-117
93%

Seq ID NO: 46459
203-386
gi|18081711
111-294
86%

Seq ID NO: 46459
1-35
gi|54549088
64-98
94%

Seq ID NO: 46459
14-35
gi|54549319
1-22
100%

Seq ID NO: 65643
1-194
gi|33140010
238-431
96%

Seq ID NO: 65643
121-194
gi|33139314
1-74
97%

Seq ID NO: 46465
159-238
gi|9829340
394-315
87%

Seq ID NO: 65738
166-205
gi|33140813
1-40
95%

Seq ID NO: 65755
83-126
gi|551594
1028-1070
93%

Seq ID NO: 65755
83-126
gi|551595
267-309
93%

Seq ID NO: 65813
1-121
gi|28916076
122-1
91%

Seq ID NO: 65813
101-126
gi|159473
61-36
100%

Seq ID NO: 65813
101-126
gi|2454547
200-225
100%

Seq ID NO: 65813
101-126
gi|551594
221-196
100%

Seq ID NO: 65813
101-126
gi|551595
696-671
100%

Seq ID NO: 65813
101-126
gi|18032254
61-36
100%

Seq ID NO: 65813
101-124
gi|18477256
256-279
100%

Seq ID NO: 65813
101-124
gi|18477260
302-325
100%

Seq ID NO: 65813
101-124
gi|18477262
611-634
100%

Seq ID NO: 65831
80-203
gi|33140348
128-252
92%

Seq ID NO: 65932
77-335
gi|33139639
245-503
87%

Seq ID NO: 65932
70-225
gi|33140673
199-44
88%

Seq ID NO: 65932
245-349
gi|32324211
165-269
86%

Seq ID NO: 65932
293-327
gi|33139131
293-327
94%

Seq ID NO: 65966
1-215
gi|33140309
306-520
97%

Seq ID NO: 66029
1-143
gi|16797830
62-204
98%

Seq ID NO: 66029
1-143
gi|16797831
62-202
97%

Seq ID NO: 66029
1-143
gi|16797832
63-203
97%

Seq ID NO: 66029
1-143
gi|26000759
62-202
96%

Seq ID NO: 66029
1-142
gi|26000762
62-201
95%

Seq ID NO: 66029
1-143
gi|31442322
62-202
95%

Seq ID NO: 66029
1-143
gi|38096133
62-202
93%

Seq ID NO: 46479
143-293
gi|18090185
96-246
90%

Seq ID NO: 46479
412-474
gi|18090185
320-382
92%

Seq ID NO: 66115
83-126
gi|551594
1028-1070
93%

Seq ID NO: 66115
83-126
gi|551595
267-309
93%

Seq ID NO: 66138
21-168
gi|54546322
121-268
87%

Seq ID NO: 66223
311-331
gi|32184515
225-245
100%

Seq ID NO: 66245
118-139
gi|54544778
203-224
100%

Seq ID NO: 46493
113-266
gi|33139869
326-476
92%

Seq ID NO: 46493
115-269
gi|18080529
142-293
90%

Seq ID NO: 46493
113-240
gi|32325143
467-591
91%

Seq ID NO: 46493
113-229
gi|33139507
467-580
92%

Seq ID NO: 46493
113-236
gi|33139658
467-587
91%

Seq ID NO: 46493
113-217
gi|33139078
467-568
91%

Seq ID NO: 46493
171-286
gi|7143888
280-392
89%

Seq ID NO: 46493
113-216
gi|33140145
467-567
91%

Seq ID NO: 46493
186-269
gi|18080754
174-257
92%

Seq ID NO: 46493
144-269
gi|52129866
585-708
86%

Seq ID NO: 46493
192-286
gi|18080844
1-95
89%

Seq ID NO: 46493
144-254
gi|52129769
700-808
87%

Seq ID NO: 46493
113-190
gi|32324728
467-544
92%

Seq ID NO: 46493
113-204
gi|33140539
467-555
90%

Seq ID NO: 46493
118-239
gi|19264211
454-572
82%

Seq ID NO: 46493
211-245
gi|22140574
144-178
97%

Seq ID NO: 46493
220-254
gi|52129262
3-37
94%

Seq ID NO: 46493
113-182
gi|15784199
440-509
84%

Seq ID NO: 46493
113-148
gi|15785306
458-492
91%

Seq ID NO: 66327
1-81
gi|35504440
192-272
95%

Seq ID NO: 66372
317-509
gi|54546686
284-476
85%

Seq ID NO: 66372
454-509
gi|33139916
1-56
100%

Seq ID NO: 66454
23-43
gi|21285223
168-148
100%

Seq ID NO: 66513
28-80
gi|54545135
100-152
96%

Seq ID NO: 66513
154-198
gi|54549414
410-454
93%

Seq ID NO: 66513
38-80
gi|54548968
1-43
95%

Seq ID NO: 66611
1-107
gi|33140780
433-539
98%

Seq ID NO: 46522
1-101
gi|18382566
65-165
89%

Seq ID NO: 46522
6-101
gi|18089815
1-96
88%

Seq ID NO: 46522
64-98
gi|21493534
40-74
91%

Seq ID NO: 66645
80-246
gi|32324211
1-167
86%

Seq ID NO: 66645
69-219
gi|33140673
199-49
83%

Seq ID NO: 66645
87-219
gi|33139639
256-388
83%

Seq ID NO: 66691
1-141
gi|16797830
62-202
98%

Seq ID NO: 66691
1-141
gi|16797831
62-200
97%

Seq ID NO: 66691
1-141
gi|16797832
63-201
97%

Seq ID NO: 66691
1-141
gi|26000759
62-200
96%

Seq ID NO: 66691
1-141
gi|31442322
62-200
95%

Seq ID NO: 66691
1-141
gi|38096133
62-200
93%

Seq ID NO: 66692
1-160
gi|5107411
189-30
100%

Seq ID NO: 66692
1-160
gi|2149587
189-30
95%

Seq ID NO: 66692
47-160
gi|34105813
1719-1606
100%

Seq ID NO: 66692
1-160
gi|2149585
189-30
92%

Seq ID NO: 66692
1-160
gi|2738785
190-30
92%

Seq ID NO: 66692
1-160
gi|2738792
190-30
92%

Seq ID NO: 66692
1-160
gi|2738799
190-30
92%

Seq ID NO: 66692
1-160
gi|2738800
190-30
92%

Seq ID NO: 66692
2-160
gi|2707748
1766-1607
90%

Seq ID NO: 66692
1-80
gi|48479719
191-112
98%

Seq ID NO: 66692
1-80
gi|37674501
194-115
98%

Seq ID NO: 66692
115-160
gi|37674501
75-30
95%

Seq ID NO: 66692
1-78
gi|1147729
2525-2448
98%

Seq ID NO: 66692
115-160
gi|1147729
2413-2368
97%

Seq ID NO: 66692
1-78
gi|2232021
87-10
98%

Seq ID NO: 66718
182-203
gi|15766531
77-56
100%

Seq ID NO: 66870
256-452
gi|18381486
666-469
84%

Seq ID NO: 66870
407-452
gi|18381486
257-212
95%

Seq ID NO: 67072
1-90
gi|33139333
250-161
96%

Seq ID NO: 46553
13-97
gi|54546152
237-321
94%

Seq ID NO: 67199
23-130
gi|18089328
199-92
87%

Seq ID NO: 46562
169-329
gi|18081141
23-183
90%

Seq ID NO: 67282
129-150
gi|45563616
32-53
100%

Seq ID NO: 67295
19-157
gi|54546846
257-396
85%

Seq ID NO: 46571
9-99
gi|18082482
10-100
92%

Seq ID NO: 67414
1-211
gi|35505285
2-212
99%

Seq ID NO: 67414
251-397
gi|35505285
211-357
99%

Seq ID NO: 67414
446-535
gi|35505285
357-446
87%

Seq ID NO: 67414
152-211
gi|7144110
176-235
91%

Seq ID NO: 67465
21-138
gi|28916076
1-119
91%

Seq ID NO: 67465
14-41
gi|159473
34-61
100%

Seq ID NO: 67465
14-41
gi|2454547
227-200
100%

Seq ID NO: 67465
14-41
gi|551594
194-221
100%

Seq ID NO: 67465
14-41
gi|551595
669-696
100%

Seq ID NO: 67465
14-41
gi|18032254
34-61
100%

Seq ID NO: 67465
18-41
gi|18477256
279-256
100%

Seq ID NO: 67465
18-41
gi|18477260
325-302
100%

Seq ID NO: 67465
18-41
gi|18477262
634-611
100%

Seq ID NO: 67465
14-35
gi|18477259
22-1
100%

Seq ID NO: 67465
14-35
gi|18477261
22-1
100%

Seq ID NO: 67465
14-35
gi|37780968
22-1
100%

Seq ID NO: 67477
36-173
gi|28916076
140-1
90%

Seq ID NO: 67477
157-180
gi|159473
57-34
100%

Seq ID NO: 67477
157-180
gi|2454547
204-227
100%

Seq ID NO: 67477
157-180
gi|551594
217-194
100%

Seq ID NO: 67477
157-180
gi|551595
692-669
100%

Seq ID NO: 67477
157-180
gi|18032254
57-34
100%

Seq ID NO: 67477
159-180
gi|18477259
1-22
100%

Seq ID NO: 67477
159-180
gi|18477261
1-22
100%

Seq ID NO: 67477
159-180
gi|37780968
1-22
100%

Seq ID NO: 67682
1-101
gi|7144157
39-139
97%

Seq ID NO: 67682
1-101
gi|54549688
26-126
96%

Seq ID NO: 67682
22-101
gi|18083086
614-535
96%

Seq ID NO: 67682
25-101
gi|18090403
1-77
96%

Seq ID NO: 67682
54-75
gi|52129301
340-319
100%

Seq ID NO: 67725
80-268
gi|33139635
1-188
96%

Seq ID NO: 67725
324-389
gi|33139635
188-253
98%

Seq ID NO: 67725
81-268
gi|32325323
1-188
93%

Seq ID NO: 67762
5-150
gi|18081743
87-232
83%

Seq ID NO: 67856
66-470
gi|33140136
92-496
90%

Seq ID NO: 67953
1-45
gi|33139122
150-194
97%

Seq ID NO: 67954
469-489
gi|19384388
444-464
100%

Seq ID NO: 67956
88-241
gi|32324211
1-154
89%

Seq ID NO: 67956
82-233
gi|33139131
99-250
86%

Seq ID NO: 67956
312-349
gi|33139131
293-330
94%

Seq ID NO: 67956
77-227
gi|33140673
199-49
86%

Seq ID NO: 67956
95-227
gi|33139639
256-388
85%

Seq ID NO: 67994
273-323
gi|33139445
12-62
96%

Seq ID NO: 67996
1-157
gi|32325279
266-422
98%

Seq ID NO: 68073
21-187
gi|7619738
1078-1244
88%

Seq ID NO: 68073
119-200
gi|33139362
1-82
100%

Seq ID NO: 68073
12-164
gi|1235973
712-864
88%

Seq ID NO: 68073
12-164
gi|54547532
371-523
87%

Seq ID NO: 68073
82-164
gi|54549430
381-463
91%

Seq ID NO: 68073
92-114
gi|6382623
174-152
100%

Seq ID NO: 68073
92-114
gi|23259469
284-306
100%

Seq ID NO: 68317
36-174
gi|28916076
140-1
90%

Seq ID NO: 68317
161-181
gi|551595
689-669
100%

Seq ID NO: 68317
161-181
gi|30168621
52-32
100%

Seq ID NO: 46608
1-166
gi|18081390
329-164
87%

Seq ID NO: 46608
1-166
gi|54545329
221-386
86%

Seq ID NO: 46608
15-116
gi|21056382
128-229
85%

Seq ID NO: 68351
4-128
gi|18083059
281-405
85%

Seq ID NO: 68370
4-68
gi|33140590
143-79
98%

Seq ID NO: 68423
15-54
gi|28916076
1-40
97%

Seq ID NO: 68423
8-35
gi|159473
34-61
100%

Seq ID NO: 68423
8-35
gi|2454547
227-200
100%

Seq ID NO: 68423
8-35
gi|551594
194-221
100%

Seq ID NO: 68423
8-35
gi|551595
669-696
100%

Seq ID NO: 68423
8-35
gi|18032254
34-61
100%

Seq ID NO: 68423
12-35
gi|18477256
279-256
100%

Seq ID NO: 68423
12-35
gi|18477260
325-302
100%

Seq ID NO: 68423
12-35
gi|18477262
634-611
100%

Seq ID NO: 68423
8-29
gi|18477259
22-1
100%

Seq ID NO: 68423
8-29
gi|18477261
22-1
100%

Seq ID NO: 68423
8-29
gi|37780968
22-1
100%

Seq ID NO: 68429
22-42
gi|28916076
280-260
100%

Seq ID NO: 68464
546-566
gi|46985670
328-348
100%

Seq ID NO: 68514
1-59
gi|32324676
60-118
96%

Seq ID NO: 68541
305-325
gi|19103835
404-424
100%

Seq ID NO: 68615
21-138
gi|28916076
1-119
91%

Seq ID NO: 68615
14-41
gi|159473
34-61
100%

Seq ID NO: 68615
14-41
gi|2454547
227-200
100%

Seq ID NO: 68615
14-41
gi|551594
194-221
100%

Seq ID NO: 68615
14-41
gi|551595
669-696
100%

Seq ID NO: 68615
14-41
gi|18032254
34-61
100%

Seq ID NO: 68615
18-41
gi|18477256
279-256
100%

Seq ID NO: 68615
18-41
gi|18477260
325-302
100%

Seq ID NO: 68615
18-41
gi|18477262
634-611
100%

Seq ID NO: 68615
14-35
gi|18477259
22-1
100%

Seq ID NO: 68615
14-35
gi|18477261
22-1
100%

Seq ID NO: 68615
14-35
gi|37780968
22-1
100%

Seq ID NO: 68654
317-337
gi|30165848
173-193
100%

Seq ID NO: 68761
1-197
gi|33139403
83-280
89%

Seq ID NO: 68761
515-623
gi|33139403
421-528
85%

Seq ID NO: 68761
45-157
gi|54546450
388-500
85%

Seq ID NO: 68783
328-492
gi|33140476
83-247
99%

Seq ID NO: 68783
129-212
gi|33140476
1-84
100%

Seq ID NO: 68783
653-732
gi|33140476
245-324
98%

Seq ID NO: 68833
21-138
gi|28916076
1-119
91%

Seq ID NO: 68833
14-41
gi|159473
34-61
100%

Seq ID NO: 68833
14-41
gi|2454547
227-200
100%

Seq ID NO: 68833
14-41
gi|551594
194-221
100%

Seq ID NO: 68833
14-41
gi|551595
669-696
100%

Seq ID NO: 68833
14-41
gi|18032254
34-61
100%

Seq ID NO: 68833
18-41
gi|18477256
279-256
100%

Seq ID NO: 68833
18-41
gi|18477260
325-302
100%

Seq ID NO: 68833
18-41
gi|18477262
634-611
100%

Seq ID NO: 68833
14-35
gi|18477259
22-1
100%

Seq ID NO: 68833
14-35
gi|18477261
22-1
100%

Seq ID NO: 68833
14-35
gi|37780968
22-1
100%

Seq ID NO: 46630
218-408
gi|33139957
1-193
98%

Seq ID NO: 46630
486-631
gi|33139957
192-337
98%

Seq ID NO: 46630
659-791
gi|33139957
325-457
98%

Seq ID NO: 46630
838-944
gi|33139957
452-558
96%

Seq ID NO: 46630
218-406
gi|33139361
1-191
97%

Seq ID NO: 46630
487-631
gi|33139361
193-337
98%

Seq ID NO: 46630
838-887
gi|33139361
452-501
96%

Seq ID NO: 69016
185-264
gi|18383042
974-895
85%

Seq ID NO: 69016
230-264
gi|18383054
1411-1445
97%

Seq ID NO: 69046
16-120
gi|28916076
252-352
88%

Seq ID NO: 69089
15-54
gi|28916076
1-40
97%

Seq ID NO: 69089
8-35
gi|159473
34-61
100%

Seq ID NO: 69089
8-35
gi|2454547
227-200
100%

Seq ID NO: 69089
8-35
gi|551594
194-221
100%

Seq ID NO: 69089
8-35
gi|551595
669-696
100%

Seq ID NO: 69089
8-35
gi|18032254
34-61
100%

Seq ID NO: 69089
12-35
gi|18477256
279-256
100%

Seq ID NO: 69089
12-35
gi|18477260
325-302
100%

Seq ID NO: 69089
12-35
gi|18477262
634-611
100%

Seq ID NO: 69089
8-29
gi|18477259
22-1
100%

Seq ID NO: 69089
8-29
gi|18477261
22-1
100%

Seq ID NO: 69089
8-29
gi|37780968
22-1
100%

Seq ID NO: 69170
15-54
gi|28916076
1-40
97%

Seq ID NO: 69170
8-35
gi|159473
34-61
100%

Seq ID NO: 69170
8-35
gi|2454547
227-200
100%

Seq ID NO: 69170
8-35
gi|551594
194-221
100%

Seq ID NO: 69170
8-35
gi|551595
669-696
100%

Seq ID NO: 69170
8-35
gi|18032254
34-61
100%

Seq ID NO: 69170
12-35
gi|18477256
279-256
100%

Seq ID NO: 69170
12-35
gi|18477260
325-302
100%

Seq ID NO: 69170
12-35
gi|18477262
634-611
100%

Seq ID NO: 69170
8-29
gi|18477259
22-1
100%

Seq ID NO: 69170
8-29
gi|18477261
22-1
100%

Seq ID NO: 69170
8-29
gi|37780968
22-1
100%

Seq ID NO: 69314
36-174
gi|28916076
140-1
92%

Seq ID NO: 69314
154-181
gi|159473
61-34
100%

Seq ID NO: 69314
154-181
gi|2454547
200-227
100%

Seq ID NO: 69314
154-181
gi|551594
221-194
100%

Seq ID NO: 69314
154-181
gi|551595
696-669
100%

Seq ID NO: 69314
154-181
gi|18032254
61-34
100%

Seq ID NO: 69314
154-177
gi|18477256
256-279
100%

Seq ID NO: 69314
154-177
gi|18477260
302-325
100%

Seq ID NO: 69314
154-177
gi|18477262
611-634
100%

Seq ID NO: 69314
160-181
gi|18477259
1-22
100%

Seq ID NO: 69314
160-181
gi|18477261
1-22
100%

Seq ID NO: 69314
160-181
gi|37780968
1-22
100%

Seq ID NO: 69324
1-369
gi|51093880
722-352
84%

Seq ID NO: 69324
1-369
gi|51093884
722-352
83%

Seq ID NO: 69324
1-252
gi|51093883
723-469
85%

Seq ID NO: 69324
313-369
gi|51093883
407-352
91%

Seq ID NO: 69324
134-369
gi|51093882
587-352
84%

Seq ID NO: 69324
134-369
gi|54545658
621-387
83%

Seq ID NO: 69327
1-128
gi|33139874
74-201
90%

Seq ID NO: 69351
1-124
gi|33140792
30-153
98%

Seq ID NO: 69351
43-124
gi|45643642
1-82
98%

Seq ID NO: 69351
58-124
gi|45643646
1-67
92%

Seq ID NO: 46651
3-78
gi|18081363
236-310
96%

Seq ID NO: 46651
3-76
gi|54544882
242-315
95%

Seq ID NO: 69410
1-141
gi|16797830
62-202
98%

Seq ID NO: 69410
1-141
gi|16797831
62-200
97%

Seq ID NO: 69410
1-141
gi|16797832
63-201
97%

Seq ID NO: 69410
1-141
gi|26000759
62-200
96%

Seq ID NO: 69410
1-141
gi|31442322
62-200
95%

Seq ID NO: 69410
1-141
gi|38096133
62-200
93%

Seq ID NO: 69440
221-245
gi|46987193
49-25
96%

Seq ID NO: 69448
1-53
gi|18081057
88-140
94%

Seq ID NO: 69449
42-186
gi|35504946
49-193
97%

Seq ID NO: 69463
1-156
gi|54548643
39-188
87%

Seq ID NO: 69463
21-156
gi|18089480
9-138
86%

Seq ID NO: 69489
1-118
gi|16797830
147-264
99%

Seq ID NO: 69489
1-118
gi|26000761
147-262
95%

Seq ID NO: 69489
1-118
gi|16797832
148-263
94%

Seq ID NO: 69489
1-118
gi|47118286
171-286
94%

Seq ID NO: 69489
1-118
gi|31442322
147-262
94%

Seq ID NO: 69489
1-118
gi|21885259
169-284
91%

Seq ID NO: 69494
323-427
gi|33139639
245-349
90%

Seq ID NO: 69494
316-441
gi|33140673
199-74
86%

Seq ID NO: 69501
113-190
gi|54549674
422-500
94%

Seq ID NO: 69501
13-53
gi|54549674
321-361
95%

Seq ID NO: 69570
16-153
gi|7143498
58-196
85%

Seq ID NO: 69592
1-136
gi|16797830
686-821
100%

Seq ID NO: 69592
1-136
gi|16797832
684-819
100%

Seq ID NO: 69592
1-136
gi|31442320
683-818
100%

Seq ID NO: 69592
1-136
gi|26000759
682-817
99%

Seq ID NO: 69592
1-136
gi|31442322
683-818
99%

Seq ID NO: 69592
1-136
gi|38096133
685-820
97%

Seq ID NO: 69592
1-120
gi|16797846
702-824
90%

Seq ID NO: 69592
1-120
gi|16797847
692-814
90%

Seq ID NO: 69592
1-120
gi|14600264
704-826
90%

Seq ID NO: 69592
1-80
gi|16797841
691-769
97%

Seq ID NO: 69592
1-80
gi|16797843
725-803
97%

Seq ID NO: 69592
1-114
gi|16797848
691-807
90%

Seq ID NO: 69592
1-80
gi|16797849
692-770
97%

Seq ID NO: 69593
1-68
gi|5107411
156-89
100%

Seq ID NO: 69593
14-68
gi|34105813
1719-1665
100%

Seq ID NO: 69593
1-51
gi|2149587
156-106
100%

Seq ID NO: 69593
1-48
gi|2738785
157-110
100%

Seq ID NO: 69593
1-48
gi|2738792
157-110
100%

Seq ID NO: 69593
1-48
gi|2738799
157-110
100%

Seq ID NO: 69593
1-48
gi|2738800
157-110
100%

Seq ID NO: 69593
1-54
gi|2149585
156-103
96%

Seq ID NO: 69593
1-47
gi|31074278
1741-1695
97%

Seq ID NO: 69593
1-47
gi|31074279
1741-1695
97%

Seq ID NO: 69593
1-47
gi|48479719
158-112
97%

Seq ID NO: 69593
1-47
gi|37674501
161-115
97%

Seq ID NO: 69593
1-45
gi|30844179
1735-1691
97%

Seq ID NO: 69716
1-121
gi|28916076
122-1
91%

Seq ID NO: 69716
101-128
gi|159473
61-34
100%

Seq ID NO: 69716
101-128
gi|2454547
200-227
100%

Seq ID NO: 69716
101-128
gi|551594
221-194
100%

Seq ID NO: 69716
101-128
gi|551595
696-669
100%

Seq ID NO: 69716
101-128
gi|18032254
61-34
100%

Seq ID NO: 69716
101-124
gi|18477256
256-279
100%

Seq ID NO: 69716
101-124
gi|18477260
302-325
100%

Seq ID NO: 69716
101-124
gi|18477262
611-634
100%

Seq ID NO: 69716
107-128
gi|18477259
1-22
100%

Seq ID NO: 69716
107-128
gi|18477261
1-22
100%

Seq ID NO: 69716
107-128
gi|37780968
1-22
100%

Seq ID NO: 69747
6-258
gi|51093884
252-7
91%

Seq ID NO: 69747
6-258
gi|18080808
295-50
89%

Seq ID NO: 69747
207-258
gi|18032252
2936-2885
92%

Seq ID NO: 69747
219-252
gi|30049837
62-29
97%

Seq ID NO: 69747
219-252
gi|20064115
400-367
94%

Seq ID NO: 69747
219-251
gi|19267510
33-1
94%

Seq ID NO: 69747
219-241
gi|19268366
27-5
100%

Seq ID NO: 69795
145-251
gi|52129366
202-308
85%

Seq ID NO: 69795
50-74
gi|52129366
161-185
100%

Seq ID NO: 69821
1-101
gi|32324237
442-541
98%

Seq ID NO: 46679
448-820
gi|33139644
1-373
93%

Seq ID NO: 46679
1053-1213
gi|33139644
387-546
95%

Seq ID NO: 69829
21-138
gi|28916076
1-119
91%

Seq ID NO: 69829
14-41
gi|159473
34-61
100%

Seq ID NO: 69829
14-41
gi|2454547
227-200
100%

Seq ID NO: 69829
14-41
gi|551594
194-221
100%

Seq ID NO: 69829
14-41
gi|551595
669-696
100%

Seq ID NO: 69829
14-41
gi|18032254
34-61
100%

Seq ID NO: 69829
18-41
gi|18477256
279-256
100%

Seq ID NO: 69829
18-41
gi|18477260
325-302
100%

Seq ID NO: 69829
18-41
gi|18477262
634-611
100%

Seq ID NO: 69829
14-35
gi|18477259
22-1
100%

Seq ID NO: 69829
14-35
gi|18477261
22-1
100%

Seq ID NO: 69829
14-35
gi|37780968
22-1
100%

Seq ID NO: 69838
1-98
gi|18382603
1075-978
89%

Seq ID NO: 69838
25-98
gi|18382603
520-447
91%

Seq ID NO: 69893
21-138
gi|28916076
1-119
91%

Seq ID NO: 69893
14-41
gi|159473
34-61
100%

Seq ID NO: 69893
14-41
gi|2454547
227-200
100%

Seq ID NO: 69893
14-41
gi|551594
194-221
100%

Seq ID NO: 69893
14-41
gi|551595
669-696
100%

Seq ID NO: 69893
14-41
gi|18032254
34-61
100%

Seq ID NO: 69893
18-41
gi|18477256
279-256
100%

Seq ID NO: 69893
18-41
gi|18477260
325-302
100%

Seq ID NO: 69893
18-41
gi|18477262
634-611
100%

Seq ID NO: 69893
14-35
gi|18477259
22-1
100%

Seq ID NO: 69893
14-35
gi|18477261
22-1
100%

Seq ID NO: 69893
14-35
gi|37780968
22-1
100%

Seq ID NO: 69930
1-65
gi|14280572
7058-7122
100%

Seq ID NO: 69972
1-247
gi|47118286
742-988
97%

Seq ID NO: 69972
1-247
gi|47118285
729-975
96%

Seq ID NO: 69972
1-230
gi|16797830
720-950
96%

Seq ID NO: 69972
1-230
gi|21885260
740-969
96%

Seq ID NO: 69972
1-230
gi|31442322
717-946
96%

Seq ID NO: 69972
1-230
gi|26000759
716-945
96%

Seq ID NO: 69972
1-230
gi|31442320
717-946
96%

Seq ID NO: 69972
1-230
gi|38096133
719-948
94%

Seq ID NO: 69972
1-44
gi|16797827
765-807
95%

Seq ID NO: 69972
142-170
gi|16797844
862-890
96%

Seq ID NO: 69972
227-247
gi|48479719
1112-1132
100%

Seq ID NO: 69978
1-157
gi|33139812
208-364
98%

Seq ID NO: 69998
1-79
gi|28916077
109-31
87%

Seq ID NO: 70006
213-380
gi|33139204
1-168
98%

Seq ID NO: 70006
96-177
gi|33139204
462-542
97%

Seq ID NO: 70006
96-214
gi|33139268
461-579
98%

Seq ID NO: 70072
1-107
gi|18087923
4-110
87%

Seq ID NO: 70072
34-68
gi|51237696
40-74
91%

Seq ID NO: 70096
21-138
gi|28916076
1-119
91%

Seq ID NO: 70096
14-41
gi|159473
34-61
100%

Seq ID NO: 70096
14-41
gi|2454547
227-200
100%

Seq ID NO: 70096
14-41
gi|551594
194-221
100%

Seq ID NO: 70096
14-41
gi|551595
669-696
100%

Seq ID NO: 70096
14-41
gi|18032254
34-61
100%

Seq ID NO: 70096
18-41
gi|18477256
279-256
100%

Seq ID NO: 70096
18-41
gi|18477260
325-302
100%

Seq ID NO: 70096
18-41
gi|18477262
634-611
100%

Seq ID NO: 70096
14-35
gi|18477259
22-1
100%

Seq ID NO: 70096
14-35
gi|18477261
22-1
100%

Seq ID NO: 70096
14-35
gi|37780968
22-1
100%

Seq ID NO: 70127
759-900
gi|33140064
109-250
98%

Seq ID NO: 70127
20-127
gi|33140064
1-108
98%

Seq ID NO: 70140
353-392
gi|32324842
178-217
97%

Seq ID NO: 70163
61-147
gi|32324657
1-89
95%

Seq ID NO: 70163
59-153
gi|32325004
1-95
92%

Seq ID NO: 70163
61-153
gi|32324353
1-93
92%

Seq ID NO: 70163
59-147
gi|33140382
1-89
93%

Seq ID NO: 70253
17-151
gi|33139131
116-250
88%

Seq ID NO: 70253
6-145
gi|32324211
1-140
88%

Seq ID NO: 70253
464-572
gi|32324211
394-502
87%

Seq ID NO: 70253
13-231
gi|33139639
256-471
81%

Seq ID NO: 70253
1-145
gi|33140673
193-49
83%

Seq ID NO: 70344
15-54
gi|28916076
1-40
97%

Seq ID NO: 70344
8-35
gi|159473
34-61
100%

Seq ID NO: 70344
8-35
gi|2454547
227-200
100%

Seq ID NO: 70344
8-35
gi|551594
194-221
100%

Seq ID NO: 70344
8-35
gi|551595
669-696
100%

Seq ID NO: 70344
8-35
gi|18032254
34-61
100%

Seq ID NO: 70344
12-35
gi|18477256
279-256
100%

Seq ID NO: 70344
12-35
gi|18477260
325-302
100%

Seq ID NO: 70344
12-35
gi|18477262
634-611
100%

Seq ID NO: 70344
8-29
gi|18477259
22-1
100%

Seq ID NO: 70344
8-29
gi|18477261
22-1
100%

Seq ID NO: 70344
8-29
gi|37780968
22-1
100%

Seq ID NO: 70357
1-28
gi|33139488
28-1
100%

Seq ID NO: 70376
160-259
gi|18090001
348-447
89%

Seq ID NO: 46707
552-763
gi|18089681
57-268
85%

Seq ID NO: 46707
463-495
gi|18089681
21-53
96%

Seq ID NO: 70457
14-41
gi|159473
34-61
100%

Seq ID NO: 70457
14-41
gi|2454547
227-200
100%

Seq ID NO: 70457
14-41
gi|551594
194-221
100%

Seq ID NO: 70457
14-41
gi|551595
669-696
100%

Seq ID NO: 70457
14-41
gi|18032254
34-61
100%

Seq ID NO: 70457
18-41
gi|18477256
279-256
100%

Seq ID NO: 70457
18-41
gi|18477260
325-302
100%

Seq ID NO: 70457
18-41
gi|18477262
634-611
100%

Seq ID NO: 70457
14-35
gi|18477259
22-1
100%

Seq ID NO: 70457
14-35
gi|18477261
22-1
100%

Seq ID NO: 70457
14-35
gi|37780968
22-1
100%

Seq ID NO: 70484
210-232
gi|18382755
877-899
100%

Seq ID NO: 70513
141-344
gi|33139742
77-280
98%

Seq ID NO: 70513
515-654
gi|33139742
368-507
95%

Seq ID NO: 70513
384-471
gi|33139742
280-367
95%

Seq ID NO: 70513
701-771
gi|33139742
510-579
97%

Seq ID NO: 70513
25-97
gi|33139742
5-77
94%

Seq ID NO: 70575
36-174
gi|28916076
140-1
91%

Seq ID NO: 70575
156-181
gi|159473
59-34
100%

Seq ID NO: 70575
156-181
gi|2454547
202-227
100%

Seq ID NO: 70575
156-181
gi|551594
219-194
100%

Seq ID NO: 70575
156-181
gi|551595
694-669
100%

Seq ID NO: 70575
156-181
gi|18032254
59-34
100%

Seq ID NO: 70575
160-181
gi|18477259
1-22
100%

Seq ID NO: 70575
156-177
gi|18477257
260-281
100%

Seq ID NO: 70575
156-177
gi|18477260
304-325
100%

Seq ID NO: 70575
160-181
gi|18477261
1-22
100%

Seq ID NO: 70575
156-177
gi|18477262
613-634
100%

Seq ID NO: 70575
160-181
gi|37780968
1-22
100%

Seq ID NO: 70581
1-143
gi|16797830
62-204
98%

Seq ID NO: 70581
1-143
gi|16797831
62-202
97%

Seq ID NO: 70581
1-143
gi|16797832
63-203
97%

Seq ID NO: 70581
1-143
gi|26000759
62-202
96%

Seq ID NO: 70581
1-142
gi|26000762
62-201
95%

Seq ID NO: 70581
1-143
gi|31442322
62-202
95%

Seq ID NO: 70581
1-143
gi|38096133
62-202
93%

Seq ID NO: 70595
116-138
gi|27925886
275-297
100%

Seq ID NO: 70736
24-50
gi|32324959
70-44
100%

Seq ID NO: 70765
327-392
gi|18082500
283-348
90%

Seq ID NO: 70765
4-65
gi|18082500
9-69
87%

Seq ID NO: 70808
1-78
gi|33140673
126-49
91%

Seq ID NO: 70808
1-110
gi|33139639
311-420
84%

Seq ID NO: 70808
19-78
gi|33139131
185-244
86%

Seq ID NO: 70815
1-122
gi|28916076
122-1
85%

Seq ID NO: 70815
106-129
gi|159473
57-34
100%

Seq ID NO: 70815
106-129
gi|2454547
204-227
100%

Seq ID NO: 70815
106-129
gi|551594
217-194
100%

Seq ID NO: 70815
106-129
gi|551595
692-669
100%

Seq ID NO: 70815
106-129
gi|18032254
57-34
100%

Seq ID NO: 70815
108-129
gi|18477259
1-22
100%

Seq ID NO: 70815
108-129
gi|18477261
1-22
100%

Seq ID NO: 70815
108-129
gi|37780968
1-22
100%

Seq ID NO: 70833
21-138
gi|28916076
1-119
91%

Seq ID NO: 70833
14-41
gi|159473
34-61
100%

Seq ID NO: 70833
14-41
gi|2454547
227-200
100%

Seq ID NO: 70833
14-41
gi|551594
194-221
100%

Seq ID NO: 70833
14-41
gi|551595
669-696
100%

Seq ID NO: 70833
14-41
gi|18032254
34-61
100%

Seq ID NO: 70833
18-41
gi|18477256
279-256
100%

Seq ID NO: 70833
18-41
gi|18477260
325-302
100%

Seq ID NO: 70833
18-41
gi|18477262
634-611
100%

Seq ID NO: 70833
14-35
gi|18477259
22-1
100%

Seq ID NO: 70833
14-35
gi|18477261
22-1
100%

Seq ID NO: 70833
14-35
gi|37780968
22-1
100%

Seq ID NO: 70838
1-119
gi|30169344
49-167
99%

Seq ID NO: 70968
1-78
gi|33140331
512-592
90%

Seq ID NO: 70972
21-138
gi|28916076
1-119
91%

Seq ID NO: 70972
14-41
gi|159473
34-61
100%

Seq ID NO: 70972
14-41
gi|2454547
227-200
100%

Seq ID NO: 70972
14-41
gi|551594
194-221
100%

Seq ID NO: 70972
14-41
gi|551595
669-696
100%

Seq ID NO: 70972
14-41
gi|18032254
34-61
100%

Seq ID NO: 70972
18-41
gi|18477256
279-256
100%

Seq ID NO: 70972
18-41
gi|18477260
325-302
100%

Seq ID NO: 70972
18-41
gi|18477262
634-611
100%

Seq ID NO: 70972
14-35
gi|18477259
22-1
100%

Seq ID NO: 70972
14-35
gi|18477261
22-1
100%

Seq ID NO: 70972
14-35
gi|37780968
22-1
100%

Seq ID NO: 70987
1-88
gi|32324011
417-505
94%

Seq ID NO: 70987
137-175
gi|32324011
506-544
100%

Seq ID NO: 46732
61-204
gi|18081136
58-201
82%

Seq ID NO: 71126
363-383
gi|19103835
404-424
100%

Seq ID NO: 71130
1-173
gi|33140136
324-496
88%

Seq ID NO: 71131
1-173
gi|33140136
324-496
88%

Seq ID NO: 71227
2-327
gi|16797830
368-43
98%

Seq ID NO: 71227
2-327
gi|47118285
378-55
96%

Seq ID NO: 71227
2-327
gi|47118286
390-67
96%

Seq ID NO: 71227
2-326
gi|31442320
365-44
96%

Seq ID NO: 71227
2-326
gi|16797832
367-45
95%

Seq ID NO: 71227
2-327
gi|21885260
388-67
95%

Seq ID NO: 71227
2-327
gi|31442322
366-43
95%

Seq ID NO: 71227
2-327
gi|38096133
366-43
92%

Seq ID NO: 71227
18-165
gi|31442314
354-207
86%

Seq ID NO: 71227
18-127
gi|38096139
352-244
88%

Seq ID NO: 71227
18-116
gi|31442318
356-259
89%

Seq ID NO: 71227
18-80
gi|16797825
322-260
95%

Seq ID NO: 71227
18-80
gi|31442305
344-282
95%

Seq ID NO: 71227
18-77
gi|16797844
348-289
95%

Seq ID NO: 71227
20-100
gi|28627583
300-220
89%

Seq ID NO: 71227
18-127
gi|31442313
354-246
85%

Seq ID NO: 71249
1-138
gi|33140698
375-512
97%

Seq ID NO: 71249
147-190
gi|33140698
521-564
97%

Seq ID NO: 71288
1-134
gi|16797830
935-802
97%

Seq ID NO: 71288
1-134
gi|26000759
931-798
97%

Seq ID NO: 71288
1-134
gi|31442322
932-799
97%

Seq ID NO: 71288
1-134
gi|16797832
933-800
96%

Seq ID NO: 71288
1-134
gi|47118286
957-824
96%

Seq ID NO: 71288
1-122
gi|26000763
930-809
97%

Seq ID NO: 71288
1-133
gi|16797831
933-801
95%

Seq ID NO: 71288
1-134
gi|38096133
934-801
94%

Seq ID NO: 71288
51-75
gi|16797844
886-862
100%

Seq ID NO: 71288
51-72
gi|16797845
888-867
100%

Seq ID NO: 46750
397-420
gi|52127553
72-95
100%

Seq ID NO: 46750
12-32
gi|403087
324-344
100%

Seq ID NO: 46750
12-32
gi|37517247
324-344
100%

Seq ID NO: 46750
12-32
gi|54548553
9-29
100%

Seq ID NO: 71318
153-177
gi|54547857
370-345
96%

Seq ID NO: 71359
1-93
gi|32324127
486-578
98%

Seq ID NO: 71359
140-211
gi|18089994
172-243
88%

Seq ID NO: 71371
21-138
gi|28916076
1-119
91%

Seq ID NO: 71371
14-41
gi|159473
34-61
100%

Seq ID NO: 71371
14-41
gi|2454547
227-200
100%

Seq ID NO: 71371
14-41
gi|551594
194-221
100%

Seq ID NO: 71371
14-41
gi|551595
669-696
100%

Seq ID NO: 71371
14-41
gi|18032254
34-61
100%

Seq ID NO: 71371
18-41
gi|18477256
279-256
100%

Seq ID NO: 71371
18-41
gi|18477260
325-302
100%

Seq ID NO: 71371
18-41
gi|18477262
634-611
100%

Seq ID NO: 71371
14-35
gi|18477259
22-1
100%

Seq ID NO: 71371
14-35
gi|18477261
22-1
100%

Seq ID NO: 71371
14-35
gi|37780968
22-1
100%

Seq ID NO: 71428
4-75
gi|33139530
421-350
100%

Seq ID NO: 71428
4-75
gi|18080188
489-418
98%

Seq ID NO: 71428
4-71
gi|54545749
478-411
97%

Seq ID NO: 71428
25-75
gi|54545112
474-423
96%

Seq ID NO: 71446
15-54
gi|28916076
1-40
97%

Seq ID NO: 71446
8-35
gi|159473
34-61
100%

Seq ID NO: 71446
8-35
gi|2454547
227-200
100%

Seq ID NO: 71446
8-35
gi|551594
194-221
100%

Seq ID NO: 71446
8-35
gi|551595
669-696
100%

Seq ID NO: 71446
8-35
gi|18032254
34-61
100%

Seq ID NO: 71446
12-35
gi|18477256
279-256
100%

Seq ID NO: 71446
12-35
gi|18477260
325-302
100%

Seq ID NO: 71446
12-35
gi|18477262
634-611
100%

Seq ID NO: 71446
8-29
gi|18477259
22-1
100%

Seq ID NO: 71446
8-29
gi|18477261
22-1
100%

Seq ID NO: 71446
8-29
gi|37780968
22-1
100%

Seq ID NO: 71587
297-344
gi|33139758
490-537
93%

Seq ID NO: 71587
300-344
gi|33140062
109-61
89%

Seq ID NO: 71599
1-184
gi|33139461
183-366
99%

Seq ID NO: 71682
1-47
gi|33139445
16-62
91%

Seq ID NO: 71689
86-112
gi|32324223
1-27
100%

Seq ID NO: 71734
1-156
gi|7144157
23-179
93%

Seq ID NO: 71734
1-156
gi|54549688
10-166
92%

Seq ID NO: 71734
38-156
gi|18083086
614-495
91%

Seq ID NO: 71734
41-156
gi|18090403
1-117
91%

Seq ID NO: 71734
1-91
gi|52129301
409-319
85%

Seq ID NO: 71797
19-95
gi|18081000
336-260
92%

Seq ID NO: 71818
1-136
gi|16797830
686-821
99%

Seq ID NO: 71818
1-136
gi|16797832
684-819
99%

Seq ID NO: 71818
1-136
gi|31442320
683-818
99%

Seq ID NO: 71818
1-136
gi|26000759
682-817
98%

Seq ID NO: 71818
1-136
gi|31442322
683-818
98%

Seq ID NO: 71818
1-136
gi|38096133
685-820
97%

Seq ID NO: 71818
1-80
gi|16797841
691-769
97%

Seq ID NO: 71818
1-80
gi|16797843
725-803
97%

Seq ID NO: 71818
1-98
gi|16797846
702-801
93%

Seq ID NO: 71818
1-98
gi|16797847
692-791
93%

Seq ID NO: 71818
1-98
gi|16797848
691-790
93%

Seq ID NO: 71818
1-80
gi|16797849
692-770
97%

Seq ID NO: 71818
1-98
gi|14600264
704-803
93%

Seq ID NO: 71848
46-108
gi|28916076
140-79
92%

Seq ID NO: 71985
33-55
gi|32325183
375-397
100%

Seq ID NO: 71996
1-141
gi|16797830
62-202
98%

Seq ID NO: 71996
1-141
gi|16797831
62-200
97%

Seq ID NO: 71996
1-141
gi|16797832
63-201
97%

Seq ID NO: 71996
1-141
gi|26000759
62-200
96%

Seq ID NO: 71996
1-141
gi|31442322
62-200
95%

Seq ID NO: 71996
1-141
gi|38096133
62-200
93%

Seq ID NO: 72109
318-338
gi|24467850
41-21
100%

Seq ID NO: 72212
1-47
gi|51093880
468-514
95%

Seq ID NO: 72212
1-39
gi|51093879
469-507
97%

Seq ID NO: 72212
1-38
gi|51093883
469-506
97%

Seq ID NO: 72222
1-181
gi|51093884
193-12
95%

Seq ID NO: 72222
1-181
gi|18080808
236-55
93%

Seq ID NO: 72222
129-180
gi|18032252
2942-2891
92%

Seq ID NO: 72222
130-180
gi|30049837
79-29
90%

Seq ID NO: 72222
147-180
gi|31326537
104-71
97%

Seq ID NO: 72222
130-180
gi|20064115
417-367
88%

Seq ID NO: 72222
130-179
gi|19267510
50-1
88%

Seq ID NO: 72222
130-169
gi|19268366
44-5
90%

Seq ID NO: 72288
5-86
gi|54546009
456-374
87%

Seq ID NO: 72350
1-57
gi|35505078
6-62
96%

Seq ID NO: 72350
21-57
gi|35504499
1-37
100%

Seq ID NO: 72377
36-174
gi|28916076
140-1
92%

Seq ID NO: 72377
154-181
gi|159473
61-34
100%

Seq ID NO: 72377
154-181
gi|2454547
200-227
100%

Seq ID NO: 72377
154-181
gi|551594
221-194
100%

Seq ID NO: 72377
154-181
gi|551595
696-669
100%

Seq ID NO: 72377
154-181
gi|18032254
61-34
100%

Seq ID NO: 72377
154-177
gi|18477256
256-279
100%

Seq ID NO: 72377
154-177
gi|18477260
302-325
100%

Seq ID NO: 72377
154-177
gi|18477262
611-634
100%

Seq ID NO: 72377
160-181
gi|18477259
1-22
100%

Seq ID NO: 72377
160-181
gi|18477261
1-22
100%

Seq ID NO: 72377
160-181
gi|37780968
1-22
100%

Seq ID NO: 72416
345-500
gi|18089572
129-284
82%

Seq ID NO: 72426
15-54
gi|28916076
1-40
97%

Seq ID NO: 72426
8-35
gi|159473
34-61
100%

Seq ID NO: 72426
8-35
gi|2454547
227-200
100%

Seq ID NO: 72426
8-35
gi|551594
194-221
100%

Seq ID NO: 72426
8-35
gi|551595
669-696
100%

Seq ID NO: 72426
8-35
gi|18032254
34-61
100%

Seq ID NO: 72426
12-35
gi|18477256
279-256
100%

Seq ID NO: 72426
12-35
gi|18477260
325-302
100%

Seq ID NO: 72426
12-35
gi|18477262
634-611
100%

Seq ID NO: 72426
8-29
gi|18477259
22-1
100%

Seq ID NO: 72426
8-29
gi|18477261
22-1
100%

Seq ID NO: 72426
8-29
gi|37780968
22-1
100%

Seq ID NO: 72433
106-352
gi|33139639
245-488
87%

Seq ID NO: 72433
99-257
gi|33140673
199-44
85%

Seq ID NO: 72529
625-646
gi|27926103
451-472
100%

Seq ID NO: 72544
77-272
gi|33139337
247-441
97%

Seq ID NO: 72544
77-245
gi|33139239
401-569
98%

Seq ID NO: 72579
564-667
gi|18082454
313-416
89%

Seq ID NO: 46809
356-378
gi|17991863
197-219
100%

Seq ID NO: 72621
15-54
gi|28916076
1-40
97%

Seq ID NO: 72621
8-35
gi|159473
34-61
100%

Seq ID NO: 72621
8-35
gi|2454547
227-200
100%

Seq ID NO: 72621
8-35
gi|551594
194-221
100%

Seq ID NO: 72621
8-35
gi|551595
669-696
100%

Seq ID NO: 72621
8-35
gi|18032254
34-61
100%

Seq ID NO: 72621
12-35
gi|18477256
279-256
100%

Seq ID NO: 72621
12-35
gi|18477260
325-302
100%

Seq ID NO: 72621
12-35
gi|18477262
634-611
100%

Seq ID NO: 72621
8-29
gi|18477259
22-1
100%

Seq ID NO: 72621
8-29
gi|18477261
22-1
100%

Seq ID NO: 72621
8-29
gi|37780968
22-1
100%

Seq ID NO: 72635
1-96
gi|33140348
160-255
96%

Seq ID NO: 72667
361-457
gi|54547761
394-490
86%

Seq ID NO: 72706
1-121
gi|28916076
122-1
91%

Seq ID NO: 72706
101-128
gi|159473
61-34
100%

Seq ID NO: 72706
101-128
gi|2454547
200-227
100%

Seq ID NO: 72706
101-128
gi|551594
221-194
100%

Seq ID NO: 72706
101-128
gi|551595
696-669
100%

Seq ID NO: 72706
101-128
gi|18032254
61-34
100%

Seq ID NO: 72706
101-124
gi|18477256
256-279
100%

Seq ID NO: 72706
101-124
gi|18477260
302-325
100%

Seq ID NO: 72706
101-124
gi|18477262
611-634
100%

Seq ID NO: 72706
107-128
gi|18477259
1-22
100%

Seq ID NO: 72706
107-128
gi|18477261
1-22
100%

Seq ID NO: 72706
107-128
gi|37780968
1-22
100%

Seq ID NO: 72808
21-138
gi|28916076
1-119
91%

Seq ID NO: 72808
14-41
gi|159473
34-61
100%

Seq ID NO: 72808
14-41
gi|2454547
227-200
100%

Seq ID NO: 72808
14-41
gi|551594
194-221
100%

Seq ID NO: 72808
14-41
gi|551595
669-696
100%

Seq ID NO: 72808
14-41
gi|18032254
34-61
100%

Seq ID NO: 72808
18-41
gi|18477256
279-256
100%

Seq ID NO: 72808
18-41
gi|18477260
325-302
100%

Seq ID NO: 72808
18-41
gi|18477262
634-611
100%

Seq ID NO: 72808
14-35
gi|18477259
22-1
100%

Seq ID NO: 72808
14-35
gi|18477261
22-1
100%

Seq ID NO: 72808
14-35
gi|37780968
22-1
100%

Seq ID NO: 72820
5-100
gi|33139131
115-213
86%

Seq ID NO: 72859
169-270
gi|18090422
452-553
89%

Seq ID NO: 73039
1-111
gi|33139007
235-125
98%

Seq ID NO: 73039
119-235
gi|33139007
117-1
94%

Seq ID NO: 73039
132-235
gi|33140543
104-1
98%

Seq ID NO: 73156
73-218
gi|32324285
154-299
97%

Seq ID NO: 73156
266-408
gi|32324285
298-440
96%

Seq ID NO: 73156
454-534
gi|32324285
439-519
100%

Seq ID NO: 73156
454-516
gi|33139772
439-501
100%

Seq ID NO: 73170
167-212
gi|32324562
537-492
91%

Seq ID NO: 73219
223-246
gi|32325459
267-290
100%

Seq ID NO: 73233
75-95
gi|19267575
145-125
100%

Seq ID NO: 73426
36-174
gi|28916076
140-1
92%

Seq ID NO: 73426
154-181
gi|159473
61-34
100%

Seq ID NO: 73426
154-181
gi|2454547
200-227
100%

Seq ID NO: 73426
154-181
gi|551594
221-194
100%

Seq ID NO: 73426
154-181
gi|551595
696-669
100%

Seq ID NO: 73426
154-181
gi|18032254
61-34
100%

Seq ID NO: 73426
154-177
gi|18477256
256-279
100%

Seq ID NO: 73426
154-177
gi|18477260
302-325
100%

Seq ID NO: 73426
154-177
gi|18477262
611-634
100%

Seq ID NO: 73426
160-181
gi|18477259
1-22
100%

Seq ID NO: 73426
160-181
gi|18477261
1-22
100%

Seq ID NO: 73426
160-181
gi|37780968
1-22
100%

Seq ID NO: 73454
1-50
gi|33140348
252-203
98%

Seq ID NO: 46864
225-248
gi|52127553
72-95
100%

Seq ID NO: 73594
79-252
gi|32324211
1-171
84%

Seq ID NO: 73594
73-224
gi|33139131
99-250
85%

Seq ID NO: 73594
294-331
gi|33139131
293-330
94%

Seq ID NO: 73616
1-285
gi|32324082
243-527
97%

Seq ID NO: 73616
331-370
gi|32324082
527-566
100%

Seq ID NO: 46871
1109-1129
gi|15766531
77-57
100%

Seq ID NO: 46896
1-152
gi|32325576
17-168
99%

Seq ID NO: 46896
1-150
gi|54547472
44-193
90%

Seq ID NO: 46896
1-150
gi|18083068
22-171
88%

Seq ID NO: 73960
36-174
gi|28916076
140-1
92%

Seq ID NO: 73960
154-181
gi|159473
61-34
100%

Seq ID NO: 73960
154-181
gi|2454547
200-227
100%

Seq ID NO: 73960
154-181
gi|551594
221-194
100%

Seq ID NO: 73960
154-181
gi|551595
696-669
100%

Seq ID NO: 73960
154-181
gi|18032254
61-34
100%

Seq ID NO: 73960
154-177
gi|18477256
256-279
100%

Seq ID NO: 73960
154-177
gi|18477260
302-325
100%

Seq ID NO: 73960
154-177
gi|18477262
611-634
100%

Seq ID NO: 73960
160-181
gi|18477259
1-22
100%

Seq ID NO: 73960
160-181
gi|18477261
1-22
100%

Seq ID NO: 73960
160-181
gi|37780968
1-22
100%

Seq ID NO: 73986
1-101
gi|33139929
341-441
99%

Seq ID NO: 73986
151-236
gi|33139929
439-524
100%

Seq ID NO: 73986
16-101
gi|32324461
1-86
98%

Seq ID NO: 73987
4-146
gi|7144133
498-641
91%

Seq ID NO: 73987
52-152
gi|18382414
1346-1446
93%

Seq ID NO: 74514
17-120
gi|7143618
355-252
86%

Seq ID NO: 74514
155-180
gi|18080329
30-5
96%

Seq ID NO: 74514
154-187
gi|18080516
211-178
94%

Seq ID NO: 74514
155-187
gi|7143678
478-446
93%

Seq ID NO: 74633
161-181
gi|38512984
421-441
100%

Seq ID NO: 74832
1-56
gi|7641359
475-420
92%

Seq ID NO: 74832
10-53
gi|15003596
5-48
97%

Seq ID NO: 74832
1-57
gi|14085900
55-111
91%

Seq ID NO: 74832
1-53
gi|14086110
51-103
92%

Seq ID NO: 74832
1-53
gi|19265574
41-93
92%

Seq ID NO: 74832
1-57
gi|39747527
43-99
91%

Seq ID NO: 74832
1-56
gi|15767660
65-120
91%

Seq ID NO: 74832
1-56
gi|18495580
501-446
91%

Seq ID NO: 74832
16-53
gi|15003618
64-101
97%

Seq ID NO: 74832
1-53
gi|34025909
42-94
90%

Seq ID NO: 74992
72-256
gi|33139639
245-429
92%

Seq ID NO: 74992
63-214
gi|33140673
201-50
89%

Seq ID NO: 75035
76-97
gi|19266802
444-465
100%

Seq ID NO: 75105
431-590
gi|33139939
312-471
97%

Seq ID NO: 75105
240-388
gi|33139939
165-312
96%

Seq ID NO: 75105
799-910
gi|33139939
472-583
99%

Seq ID NO: 75105
64-176
gi|33139939
52-164
94%

Seq ID NO: 75105
829-959
gi|18090520
33-163
87%

Seq ID NO: 75106
327-348
gi|33140085
432-453
100%

Seq ID NO: 75118
318-457
gi|14280575
2710-2849
100%

Seq ID NO: 75118
133-231
gi|14280575
2614-2712
100%

Seq ID NO: 75118
2-77
gi|14280575
2538-2613
100%

Seq ID NO: 75118
318-355
gi|14280570
3427-3465
92%

Seq ID NO: 75173
161-181
gi|32324086
604-584
100%

Seq ID NO: 75190
67-108
gi|7144329
64-105
100%

Seq ID NO: 75203
175-233
gi|21393342
99-157
88%

Seq ID NO: 75203
190-233
gi|21393558
107-150
91%

Seq ID NO: 75203
175-201
gi|7797714
281-307
96%

Seq ID NO: 75289
66-470
gi|33140136
92-496
90%

Seq ID NO: 75439
292-321
gi|7922575
190-218
96%

Seq ID NO: 75663
438-478
gi|18081901
558-518
92%

Seq ID NO: 47026
543-822
gi|18382431
565-486
91%

Seq ID NO: 75916
57-196
gi|32323999
1-140
95%

Seq ID NO: 75950
6-261
gi|32324211
1-256
84%

Seq ID NO: 75950
17-145
gi|33139131
116-244
89%

Seq ID NO: 75950
13-250
gi|33139639
256-493
81%

Seq ID NO: 75950
1-145
gi|33140673
193-49
84%

Seq ID NO: 75960
1-75
gi|30028941
235-309
96%

Seq ID NO: 75960
1-75
gi|33140204
235-309
96%

Seq ID NO: 47033
313-382
gi|32324686
13-82
98%

Seq ID NO: 76094
66-470
gi|33140136
92-496
89%

Seq ID NO: 76242
181-203
gi|27001433
261-283
100%

Seq ID NO: 76281
16-139
gi|54546240
15-138
90%

Seq ID NO: 76281
16-139
gi|18089390
11-134
89%

Seq ID NO: 76311
128-240
gi|32324311
117-229
88%

Seq ID NO: 76311
1-46
gi|32324311
55-100
93%

Seq ID NO: 76311
130-241
gi|33140078
156-267
87%

Seq ID NO: 76311
152-241
gi|33139028
10-99
90%

Seq ID NO: 47066
255-278
gi|7143978
211-234
100%

Seq ID NO: 76358
1-57
gi|32324009
418-474
98%

Seq ID NO: 76358
1-53
gi|33139304
439-491
98%

Seq ID NO: 76409
5-25
gi|19267937
180-160
100%

Seq ID NO: 76432
620-750
gi|33139974
141-11
99%

Seq ID NO: 76432
339-516
gi|33139974
316-139
91%

Seq ID NO: 76470
296-390
gi|33140673
141-44
85%

Seq ID NO: 76471
1-87
gi|35505085
14-100
97%

Seq ID NO: 76471
8-87
gi|35504991
21-100
95%

Seq ID NO: 76471
49-87
gi|35505046
1-39
100%

Seq ID NO: 76556
591-615
gi|19103651
327-352
96%

Seq ID NO: 76634
17-151
gi|33139131
116-250
88%

Seq ID NO: 76634
6-145
gi|32324211
1-140
88%

Seq ID NO: 76634
13-279
gi|33139639
256-516
80%

Seq ID NO: 76634
1-145
gi|33140673
193-49
84%

Seq ID NO: 76667
3-64
gi|32325283
20-81
96%

Seq ID NO: 76718
1-84
gi|33139696
197-280
94%

Seq ID NO: 76775
1-149
gi|35505158
357-505
99%

Seq ID NO: 47105
82-256
gi|18080493
64-238
87%

Seq ID NO: 47105
16-36
gi|18080493
48-68
100%

Seq ID NO: 47105
82-254
gi|7144379
51-223
87%

Seq ID NO: 76970
61-82
gi|18090014
19-40
100%

Seq ID NO: 77032
570-679
gi|33139969
11-120
96%

Seq ID NO: 77038
5-123
gi|2707748
1563-1445
99%

Seq ID NO: 77038
5-123
gi|34105813
1562-1444
99%

Seq ID NO: 77038
5-123
gi|34105815
1562-1444
99%

Seq ID NO: 77038
17-123
gi|30844179
1550-1444
95%

Seq ID NO: 77038
17-123
gi|34105807
1434-1328
95%

Seq ID NO: 77038
17-123
gi|34105808
1545-1439
95%

Seq ID NO: 77038
17-123
gi|34105810
1561-1455
95%

Seq ID NO: 77038
17-123
gi|31376322
1492-1386
94%

Seq ID NO: 77038
17-123
gi|31376323
1488-1382
94%

Seq ID NO: 77038
17-123
gi|34105806
1558-1452
93%

Seq ID NO: 77038
17-123
gi|51093982
386-280
93%

Seq ID NO: 77038
17-123
gi|6983959
1511-1405
92%

Seq ID NO: 77038
17-123
gi|22544385
274-168
92%

Seq ID NO: 77038
17-123
gi|30169951
41-147
92%

Seq ID NO: 77038
17-123
gi|31376325
1503-1397
92%

Seq ID NO: 77198
308-501
gi|33140188
263-70
96%

Seq ID NO: 77198
302-469
gi|33140188
464-297
87%

Seq ID NO: 77198
4-91
gi|33140188
353-266
97%

Seq ID NO: 77198
1-88
gi|33140188
551-464
90%

Seq ID NO: 77198
389-469
gi|33140188
572-492
91%

Seq ID NO: 77198
1-60
gi|33140188
161-102
90%

Seq ID NO: 77199
5-155
gi|33140188
113-263
96%

Seq ID NO: 77199
372-521
gi|33140188
266-415
96%

Seq ID NO: 77199
403-519
gi|33140188
102-218
94%

Seq ID NO: 77199
5-161
gi|33140188
308-464
88%

Seq ID NO: 77199
375-483
gi|33140188
464-572
91%

Seq ID NO: 77199
5-74
gi|33140188
503-572
91%

Seq ID NO: 77213
199-266
gi|18090001
163-230
85%

Seq ID NO: 77253
1-146
gi|33139770
381-526
98%

Seq ID NO: 77260
4-60
gi|33140397
536-592
98%

Seq ID NO: 77260
4-58
gi|33139624
522-576
98%

Seq ID NO: 77342
1-101
gi|7144157
39-139
97%

Seq ID NO: 77342
1-101
gi|54549688
26-126
96%

Seq ID NO: 77342
22-101
gi|18083086
614-535
96%

Seq ID NO: 77342
25-101
gi|18090403
1-77
96%

Seq ID NO: 77342
54-75
gi|52129301
340-319
100%

Seq ID NO: 77382
7-92
gi|54546009
456-370
88%

Seq ID NO: 77397
2-222
gi|7144372
296-516
90%

Seq ID NO: 77397
2-214
gi|18081853
341-552
90%

Seq ID NO: 77478
1-43
gi|7144081
50-92
90%

Seq ID NO: 77496
474-494
gi|39747177
32-12
100%

Seq ID NO: 77564
203-337
gi|33140348
119-253
86%

Seq ID NO: 77649
1-160
gi|18081259
449-291
93%

Seq ID NO: 77725
1-24
gi|33139696
554-577
100%

Seq ID NO: 78000
1-163
gi|32324951
29-197
94%

Seq ID NO: 47181
709-827
gi|18080339
13-131
88%

Seq ID NO: 78139
149-236
gi|32324369
16-103
97%

Seq ID NO: 78157
789-809
gi|34025242
232-212
100%

Seq ID NO: 78157
789-809
gi|32322566
529-509
100%

Seq ID NO: 47188
202-341
gi|32324409
329-468
95%

Seq ID NO: 47188
1-90
gi|33139667
502-591
98%

Seq ID NO: 78415
107-386
gi|33140127
75-354
96%

Seq ID NO: 78415
1-44
gi|33140127
33-76
95%

Seq ID NO: 78436
8-67
gi|28916076
60-1
90%

Seq ID NO: 78818
18-77
gi|30028941
53-112
95%

Seq ID NO: 78818
18-77
gi|33140204
53-112
95%

Seq ID NO: 78927
1-110
gi|32325405
74-183
95%

Seq ID NO: 78955
118-142
gi|18089765
337-361
100%

Seq ID NO: 79093
105-368
gi|33139802
311-574
98%

Seq ID NO: 79093
1-63
gi|33139802
249-311
100%

Seq ID NO: 79093
105-344
gi|33140772
311-550
98%

Seq ID NO: 79160
206-305
gi|33139131
107-206
87%

Seq ID NO: 79160
429-458
gi|33139131
300-328
93%

Seq ID NO: 79160
291-313
gi|7143644
362-384
100%

Seq ID NO: 79239
183-270
gi|18083061
2-89
87%

Seq ID NO: 79275
94-131
gi|15767717
372-409
92%

Seq ID NO: 79292
768-882
gi|18082714
142-256
88%

Seq ID NO: 79369
124-211
gi|32325459
290-208
84%

Seq ID NO: 79371
10-86
gi|54547347
34-110
88%

Seq ID NO: 79419
1-110
gi|33139485
331-440
95%

Seq ID NO: 79419
161-248
gi|33139485
438-525
98%

Seq ID NO: 79533
1-179
gi|33140348
253-75
89%

Seq ID NO: 79533
122-160
gi|32324498
44-5
92%

Seq ID NO: 79545
1-68
gi|7143918
49-116
91%

Seq ID NO: 79545
1-42
gi|18382970
1050-1009
95%

Seq ID NO: 47286
317-516
gi|18088906
218-417
88%

Seq ID NO: 47286
106-178
gi|18088906
140-212
91%

Seq ID NO: 79576
77-189
gi|32324732
377-489
98%

Seq ID NO: 79576
1-69
gi|32324732
301-369
100%

Seq ID NO: 79602
60-139
gi|7143654
84-5
90%

Seq ID NO: 79614
287-458
gi|30169344
167-338
97%

Seq ID NO: 79614
1-119
gi|30169344
49-167
100%

Seq ID NO: 79729
1-28
gi|33140324
288-315
100%

Seq ID NO: 79741
94-257
gi|33140312
390-553
96%

Seq ID NO: 79741
94-145
gi|33140619
501-552
94%

Seq ID NO: 79818
1-75
gi|32325315
37-111
100%

Seq ID NO: 79818
18-75
gi|35504416
1-58
98%

Seq ID NO: 79818
32-75
gi|35504544
15-58
100%

Seq ID NO: 79818
36-75
gi|35504483
4-43
100%

Seq ID NO: 79885
1-141
gi|33139639
311-451
90%

Seq ID NO: 79885
1-78
gi|33140673
126-49
92%

Seq ID NO: 79885
32-78
gi|32324211
94-140
95%

Seq ID NO: 79893
1-62
gi|32324007
469-408
95%

Seq ID NO: 80017
17-50
gi|7144038
422-455
100%

Seq ID NO: 80102
5-88
gi|33139645
485-570
91%

Seq ID NO: 80192
397-417
gi|17991359
548-528
100%

Seq ID NO: 47324
1-98
gi|35504434
329-426
91%

Seq ID NO: 47325
416-525
gi|7143492
304-195
90%

Seq ID NO: 80394
1-240
gi|33139962
21-260
96%

Seq ID NO: 80394
295-405
gi|33139962
264-374
97%

Seq ID NO: 80394
199-239
gi|18089348
186-227
92%

Seq ID NO: 80422
39-59
gi|27926823
344-364
100%

Seq ID NO: 80601
50-200
gi|33140348
105-255
94%

Seq ID NO: 80601
8-63
gi|33140348
53-107
96%

Seq ID NO: 80601
50-77
gi|32324498
17-44
96%

Seq ID NO: 80750
67-235
gi|54546057
58-226
88%

Seq ID NO: 80750
12-33
gi|33952487
540-561
100%

Seq ID NO: 80750
12-33
gi|34025939
541-562
100%

Seq ID NO: 80750
205-226
gi|453579
1960-1939
100%

Seq ID NO: 80985
458-478
gi|8005746
172-192
100%

Seq ID NO: 81077
19-132
gi|18080124
72-186
84%

Seq ID NO: 81077
35-63
gi|18082691
129-157
96%

Seq ID NO: 81120
854-916
gi|32324217
4-66
100%

Seq ID NO: 81120
862-883
gi|7797944
63-84
100%

Seq ID NO: 47400
1-136
gi|33140804
236-371
100%

Seq ID NO: 47400
194-337
gi|33140804
372-515
95%

Seq ID NO: 81337
1-154
gi|32324094
298-451
97%

Seq ID NO: 81337
2-135
gi|18381585
1162-1029
87%

Seq ID NO: 81377
309-329
gi|30165848
173-193
100%

Seq ID NO: 81381
1-110
gi|33139131
114-225
87%

Seq ID NO: 81412
136-188
gi|32324211
450-502
90%

Seq ID NO: 81471
50-242
gi|18088614
91-283
87%

Seq ID NO: 81471
589-666
gi|18088614
289-366
93%

Seq ID NO: 81471
606-666
gi|32183705
270-330
88%

Seq ID NO: 81477
17-237
gi|18082509
367-587
90%

Seq ID NO: 81477
17-237
gi|54548304
360-580
90%

Seq ID NO: 81477
17-231
gi|54548230
373-587
88%

Seq ID NO: 81624
114-264
gi|33139681
415-565
98%

Seq ID NO: 81624
1-72
gi|33139681
346-417
98%

Seq ID NO: 81634
1-153
gi|7110849
31-183
91%

Seq ID NO: 81634
1-162
gi|54546003
335-496
87%

Seq ID NO: 81648
15-54
gi|28916076
1-40
97%

Seq ID NO: 81648
8-35
gi|159473
34-61
100%

Seq ID NO: 81648
8-35
gi|2454547
227-200
100%

Seq ID NO: 81648
8-35
gi|551594
194-221
100%

Seq ID NO: 81648
8-35
gi|551595
669-696
100%

Seq ID NO: 81648
8-35
gi|18032254
34-61
100%

Seq ID NO: 81648
12-35
gi|18477256
279-256
100%

Seq ID NO: 81648
12-35
gi|18477260
325-302
100%

Seq ID NO: 81648
12-35
gi|18477262
634-611
100%

Seq ID NO: 81648
8-29
gi|18477259
22-1
100%

Seq ID NO: 81648
8-29
gi|18477261
22-1
100%

Seq ID NO: 81648
8-29
gi|37780968
22-1
100%

Seq ID NO: 81702
17-91
gi|33139131
116-190
92%

Seq ID NO: 81702
6-97
gi|32324211
1-92
87%

Seq ID NO: 81703
84-347
gi|33139639
256-516
84%

Seq ID NO: 81703
77-216
gi|32324211
1-140
89%

Seq ID NO: 81703
71-222
gi|33139131
99-250
87%

Seq ID NO: 81703
66-216
gi|33140673
199-49
86%

Seq ID NO: 81813
589-938
gi|33139425
112-463
94%

Seq ID NO: 81813
429-539
gi|33139425
1-111
97%

Seq ID NO: 81813
664-822
gi|32323963
132-290
98%

Seq ID NO: 81813
877-972
gi|32323963
290-385
98%

Seq ID NO: 81813
877-971
gi|32325615
290-384
98%

Seq ID NO: 81813
427-539
gi|33139274
1-113
97%

Seq ID NO: 81813
429-606
gi|33140556
1-181
92%

Seq ID NO: 81813
666-822
gi|32324994
135-291
98%

Seq ID NO: 81813
428-539
gi|32324994
1-112
99%

Seq ID NO: 81813
877-947
gi|33139381
232-302
98%

Seq ID NO: 81813
487-539
gi|33139381
1-53
100%

Seq ID NO: 81813
427-523
gi|33140583
1-97
96%

Seq ID NO: 81817
452-473
gi|33952321
139-160
100%

Seq ID NO: 81839
16-77
gi|7143651
36-97
90%

Seq ID NO: 81895
515-875
gi|32325036
211-571
95%

Seq ID NO: 81895
472-827
gi|32325036
216-571
94%

Seq ID NO: 81895
563-897
gi|32325036
211-545
94%

Seq ID NO: 81895
514-693
gi|32325036
18-197
93%

Seq ID NO: 81895
482-645
gi|32325036
34-197
94%

Seq ID NO: 81895
610-789
gi|32325036
18-197
92%

Seq ID NO: 81895
562-741
gi|32325036
18-197
92%

Seq ID NO: 81895
658-837
gi|32325036
18-197
92%

Seq ID NO: 81895
706-885
gi|32325036
18-197
92%

Seq ID NO: 81895
754-897
gi|32325036
18-161
93%

Seq ID NO: 81895
472-597
gi|32325036
72-197
93%

Seq ID NO: 81895
48-246
gi|32325036
231-428
86%

Seq ID NO: 81895
1-246
gi|32325036
232-476
83%

Seq ID NO: 81895
137-270
gi|32325036
224-356
87%

Seq ID NO: 81895
48-207
gi|32325036
39-197
83%

Seq ID NO: 81895
138-253
gi|32325036
33-147
86%

Seq ID NO: 81895
1-157
gi|32325036
40-196
80%

Seq ID NO: 81895
470-716
gi|33140561
167-413
96%

Seq ID NO: 81895
520-764
gi|33140561
169-413
95%

Seq ID NO: 81895
568-812
gi|33140561
169-413
95%

Seq ID NO: 81895
616-860
gi|33140561
169-413
95%

Seq ID NO: 81895
664-897
gi|33140561
169-402
95%

Seq ID NO: 81895
457-668
gi|33140561
202-413
95%

Seq ID NO: 81895
1-230
gi|33140561
185-413
83%

Seq ID NO: 81895
691-712
gi|33140561
412-433
100%

Seq ID NO: 81895
835-856
gi|33140561
412-433
100%

Seq ID NO: 81895
547-568
gi|33140561
412-433
100%

Seq ID NO: 81895
787-808
gi|33140561
412-433
100%

Seq ID NO: 81895
643-664
gi|33140561
412-433
100%

Seq ID NO: 81895
739-760
gi|33140561
412-433
100%

Seq ID NO: 81895
595-616
gi|33140561
412-433
100%

Seq ID NO: 81895
499-520
gi|33140561
412-433
100%

Seq ID NO: 81895
534-896
gi|7143587
175-537
83%

Seq ID NO: 81895
486-848
gi|7143587
175-537
83%

Seq ID NO: 81895
484-752
gi|7143944
428-699
82%

Seq ID NO: 81895
724-896
gi|7143944
332-504
84%

Seq ID NO: 81895
630-728
gi|7143944
133-231
88%

Seq ID NO: 81895
582-671
gi|7143944
133-222
89%

Seq ID NO: 81895
534-623
gi|7143944
133-222
89%

Seq ID NO: 81895
774-863
gi|7143944
133-222
89%

Seq ID NO: 81895
486-575
gi|7143944
133-222
89%

Seq ID NO: 81895
822-896
gi|7143944
133-207
90%

Seq ID NO: 81895
678-767
gi|7143944
133-222
86%

Seq ID NO: 81895
470-569
gi|33139222
297-396
97%

Seq ID NO: 81895
520-617
gi|33139222
299-396
95%

Seq ID NO: 81895
760-857
gi|33139222
299-396
95%

Seq ID NO: 81895
616-713
gi|33139222
299-396
95%

Seq ID NO: 81895
568-665
gi|33139222
299-396
95%

Seq ID NO: 81895
712-809
gi|33139222
299-396
93%

Seq ID NO: 81895
664-761
gi|33139222
299-396
93%

Seq ID NO: 81895
808-897
gi|33139222
299-388
95%

Seq ID NO: 81895
457-521
gi|33139222
332-396
92%

Seq ID NO: 82088
73-93
gi|33140421
13-33
100%

Seq ID NO: 82094
18-129
gi|33139131
139-250
90%

Seq ID NO: 82094
31-123
gi|32324211
48-140
90%

Seq ID NO: 82094
1-152
gi|33139639
266-417
80%

Seq ID NO: 82109
19-196
gi|18087933
464-287
90%

Seq ID NO: 82109
19-184
gi|54547517
403-568
88%

Seq ID NO: 82109
19-166
gi|18081843
491-638
89%

Seq ID NO: 82109
19-139
gi|18083082
505-625
90%

Seq ID NO: 82109
66-191
gi|51334233
404-529
81%

Seq ID NO: 82142
1-79
gi|33140136
402-480
93%

Seq ID NO: 82163
122-267
gi|33139131
99-244
87%

Seq ID NO: 82163
128-267
gi|32324211
1-140
87%

Seq ID NO: 82166
2-106
gi|33139639
245-349
90%

Seq ID NO: 82166
1-120
gi|33140673
193-74
84%

Seq ID NO: 82199
66-470
gi|33140136
92-496
89%

Seq ID NO: 82221
1-87
gi|18090662
244-330
88%

Seq ID NO: 82256
59-122
gi|14280572
6490-6553
92%

Seq ID NO: 82260
698-718
gi|37972243
197-217
100%

Seq ID NO: 82260
698-718
gi|45566049
213-233
100%

Seq ID NO: 47476
3-211
gi|18089811
280-488
87%

Seq ID NO: 47476
3-167
gi|18080518
317-481
88%

Seq ID NO: 82506
1-174
gi|33140348
248-75
89%

Seq ID NO: 82506
117-155
gi|32324498
44-5
92%

Seq ID NO: 82529
1-86
gi|32324692
54-139
97%

Seq ID NO: 82529
7-86
gi|18082298
52-131
90%

Seq ID NO: 82799
1-40
gi|33140348
254-215
100%

Seq ID NO: 82799
78-135
gi|33140348
181-123
90%

Seq ID NO: 82849
599-663
gi|18080145
213-277
87%

Seq ID NO: 82849
358-380
gi|40670113
439-417
100%

Seq ID NO: 82865
1-127
gi|33140348
255-128
93%

Seq ID NO: 82865
185-227
gi|33140348
70-27
97%

Seq ID NO: 82929
772-793
gi|8930759
49-28
100%

Seq ID NO: 82929
772-793
gi|34026216
41-20
100%

Seq ID NO: 82965
302-322
gi|46984484
89-109
100%

Seq ID NO: 83225
316-338
gi|27540675
36-58
100%

Seq ID NO: 83226
299-358
gi|32325383
584-525
98%

Seq ID NO: 83249
3-237
gi|33139581
1-235
99%

Seq ID NO: 83274
1-55
gi|21393574
296-350
89%

Seq ID NO: 83339
1-268
gi|51093880
722-452
86%

Seq ID NO: 83339
1-252
gi|51093883
723-469
85%

Seq ID NO: 83339
134-268
gi|51093882
587-452
87%

Seq ID NO: 83339
134-252
gi|54545658
621-503
87%

Seq ID NO: 83379
40-139
gi|18081804
201-300
89%

Seq ID NO: 83379
40-130
gi|18088266
516-606
90%

Seq ID NO: 83382
42-429
gi|32325123
663-276
87%

Seq ID NO: 83382
132-519
gi|32325123
663-276
87%

Seq ID NO: 83382
1-384
gi|32325123
659-276
87%

Seq ID NO: 83382
90-474
gi|32325123
660-276
86%

Seq ID NO: 83382
3-294
gi|32325123
567-276
89%

Seq ID NO: 83382
177-526
gi|32325123
663-314
86%

Seq ID NO: 83382
1-204
gi|32325123
479-276
90%

Seq ID NO: 83382
312-530
gi|32325123
663-445
84%

Seq ID NO: 83382
1-87
gi|32325123
344-258
94%

Seq ID NO: 83382
17-165
gi|32325123
236-84
83%

Seq ID NO: 83382
308-435
gi|32325123
214-84
83%

Seq ID NO: 83382
332-384
gi|32325123
236-183
90%

Seq ID NO: 83382
377-429
gi|32325123
236-183
90%

Seq ID NO: 83382
219-345
gi|32325123
213-84
81%

Seq ID NO: 83382
489-519
gi|32325123
213-183
100%

Seq ID NO: 83382
129-159
gi|32325123
213-183
100%

Seq ID NO: 83382
17-75
gi|32325123
143-84
88%

Seq ID NO: 83382
242-294
gi|32325123
236-183
88%

Seq ID NO: 83382
197-255
gi|32325123
143-84
87%

Seq ID NO: 83382
467-525
gi|32325123
143-84
87%

Seq ID NO: 83382
1-24
gi|32325123
206-183
100%

Seq ID NO: 83382
9-30
gi|32325123
105-84
100%

Seq ID NO: 83382
129-519
gi|33139983
660-271
87%

Seq ID NO: 83382
38-519
gi|33139983
661-178
84%

Seq ID NO: 83382
78-474
gi|33139983
666-271
86%

Seq ID NO: 83382
173-526
gi|33139983
661-309
87%

Seq ID NO: 83382
308-530
gi|33139983
661-440
87%

Seq ID NO: 83382
351-519
gi|33139983
663-495
88%

Seq ID NO: 83382
1-337
gi|33139983
429-87
82%

Seq ID NO: 83382
129-513
gi|33139225
635-252
87%

Seq ID NO: 83382
1-378
gi|33139225
628-252
87%

Seq ID NO: 83382
38-423
gi|33139225
636-252
86%

Seq ID NO: 83382
82-464
gi|33139225
637-256
86%

Seq ID NO: 83382
352-519
gi|33139225
637-470
88%

Seq ID NO: 83382
3-159
gi|33139225
312-153
82%

Seq ID NO: 83382
28-384
gi|32324170
684-328
87%

Seq ID NO: 83382
129-474
gi|32324170
673-328
87%

Seq ID NO: 83382
163-519
gi|32324170
684-328
87%

Seq ID NO: 83382
81-519
gi|32324170
676-235
84%

Seq ID NO: 83382
219-528
gi|32324170
673-364
88%

Seq ID NO: 83382
3-439
gi|32324170
574-132
83%

Seq ID NO: 83382
253-526
gi|32324170
684-411
87%

Seq ID NO: 83382
1-357
gi|32324170
486-124
82%

Seq ID NO: 83382
350-530
gi|32324170
677-497
87%

Seq ID NO: 83382
1-78
gi|32324170
666-589
96%

Seq ID NO: 83382
433-528
gi|32324170
684-589
90%

Seq ID NO: 83382
222-519
gi|33140346
602-302
86%

Seq ID NO: 83382
132-429
gi|33140346
602-302
86%

Seq ID NO: 83382
42-428
gi|33140346
602-210
83%

Seq ID NO: 83382
1-338
gi|33140346
553-210
83%

Seq ID NO: 83382
357-526
gi|33140346
602-433
90%

Seq ID NO: 83382
17-87
gi|33140346
168-97
91%

Seq ID NO: 83382
377-439
gi|33140346
168-105
90%

Seq ID NO: 83382
492-528
gi|33140346
602-566
100%

Seq ID NO: 83382
467-529
gi|33140346
168-105
89%

Seq ID NO: 83382
332-394
gi|33140346
168-105
89%

Seq ID NO: 83382
107-169
gi|33140346
168-105
89%

Seq ID NO: 83382
197-259
gi|33140346
168-105
89%

Seq ID NO: 83382
377-434
gi|33140346
75-17
90%

Seq ID NO: 83382
17-74
gi|33140346
75-17
90%

Seq ID NO: 83382
1-114
gi|33140346
418-302
82%

Seq ID NO: 83382
1-34
gi|33140346
138-105
97%

Seq ID NO: 83382
332-389
gi|33140346
75-17
88%

Seq ID NO: 83382
107-164
gi|33140346
75-17
88%

Seq ID NO: 83382
467-524
gi|33140346
75-17
88%

Seq ID NO: 83382
197-254
gi|33140346
75-17
88%

Seq ID NO: 83382
1-29
gi|33140346
45-17
96%

Seq ID NO: 83382
197-384
gi|33140426
442-254
88%

Seq ID NO: 83382
332-519
gi|33140426
442-254
88%

Seq ID NO: 83382
287-474
gi|33140426
442-254
87%

Seq ID NO: 83382
242-429
gi|33140426
442-254
88%

Seq ID NO: 83382
107-294
gi|33140426
442-254
87%

Seq ID NO: 83382
1-159
gi|33140426
412-254
89%

Seq ID NO: 83382
17-204
gi|33140426
442-254
86%

Seq ID NO: 83382
377-528
gi|33140426
442-290
88%

Seq ID NO: 83382
62-249
gi|33140426
442-254
86%

Seq ID NO: 83382
3-87
gi|33140426
320-236
91%

Seq ID NO: 83382
28-169
gi|32325556
222-78
83%

Seq ID NO: 83382
298-439
gi|32325556
222-78
82%

Seq ID NO: 83382
208-357
gi|32325556
222-70
80%

Seq ID NO: 83382
478-519
gi|32325556
222-181
92%

Seq ID NO: 83382
17-153
gi|32324836
149-10
83%

Seq ID NO: 83382
377-513
gi|32324836
149-10
82%

Seq ID NO: 83382
197-329
gi|32324836
149-14
81%

Seq ID NO: 83382
107-243
gi|32324836
149-10
81%

Seq ID NO: 83382
467-519
gi|32324836
149-97
90%

Seq ID NO: 83382
1-104
gi|32324836
120-14
81%

Seq ID NO: 83382
38-161
gi|33139487
219-93
83%

Seq ID NO: 83382
350-384
gi|33139487
222-188
97%

Seq ID NO: 83382
219-339
gi|33139487
218-95
81%

Seq ID NO: 83382
398-429
gi|33139487
219-188
96%

Seq ID NO: 83382
43-153
gi|33139115
124-11
83%

Seq ID NO: 83382
133-243
gi|33139115
124-11
82%

Seq ID NO: 83382
403-513
gi|33139115
124-11
82%

Seq ID NO: 83382
223-329
gi|33139115
124-15
82%

Seq ID NO: 83382
493-519
gi|33139115
124-98
100%

Seq ID NO: 83382
358-384
gi|33139115
124-98
100%

Seq ID NO: 83407
61-104
gi|54546446
55-98
90%

Seq ID NO: 83502
3-97
gi|18090256
296-390
90%

Seq ID NO: 83531
1-55
gi|33140348
201-255
98%

Seq ID NO: 83556
31-110
gi|54547037
1-80
86%

Seq ID NO: 83556
32-110
gi|54547033
1-79
86%

Seq ID NO: 83658
281-303
gi|33138709
165-143
100%

Seq ID NO: 83658
337-357
gi|28916076
280-260
100%

Seq ID NO: 83678
289-309
gi|37853736
373-393
100%

Seq ID NO: 83731
1-134
gi|32324631
49-182
94%

Seq ID NO: 83731
1-107
gi|33139363
49-152
92%

Seq ID NO: 83737
18-39
gi|31326189
232-211
100%

Seq ID NO: 83765
226-357
gi|7144169
364-495
87%

Seq ID NO: 83765
274-335
gi|18081294
455-516
91%

Seq ID NO: 83765
274-358
gi|18089822
443-527
91%

Seq ID NO: 83781
408-429
gi|17971089
242-221
100%

Seq ID NO: 83828
539-605
gi|33139668
557-491
92%

Seq ID NO: 83828
386-419
gi|33139668
530-497
94%

Seq ID NO: 83828
347-368
gi|33139668
530-509
100%

Seq ID NO: 83828
35-56
gi|33139668
530-509
100%

Seq ID NO: 83828
230-251
gi|33139668
530-509
100%

Seq ID NO: 83828
566-605
gi|33139848
530-491
97%

Seq ID NO: 83855
27-51
gi|33140021
1-25
100%

Seq ID NO: 47578
1-101
gi|18382566
65-165
88%

Seq ID NO: 47578
7-101
gi|18089815
2-96
88%

Seq ID NO: 47578
64-98
gi|21493534
40-74
91%

Seq ID NO: 84076
75-164
gi|18081616
391-302
87%

Seq ID NO: 84205
295-402
gi|18079959
358-465
82%

Seq ID NO: 84241
159-179
gi|52127565
269-249
100%

Seq ID NO: 84390
75-97
gi|34028088
28-50
100%

Seq ID NO: 84419
109-281
gi|33139639
255-428
88%

Seq ID NO: 84419
96-242
gi|33140673
195-49
89%

Seq ID NO: 84522
1-73
gi|33140257
196-268
98%

Seq ID NO: 84522
44-73
gi|32324393
1-30
100%

Seq ID NO: 84576
73-112
gi|21393592
395-434
90%

Seq ID NO: 84645
275-323
gi|7143520
433-385
91%

Seq ID NO: 84648
6-28
gi|35504845
119-97
100%

Seq ID NO: 84664
125-168
gi|33139131
293-336
93%

Seq ID NO: 84696
527-650
gi|35504896
483-606
96%

Seq ID NO: 84696
1-127
gi|35504896
213-339
96%

Seq ID NO: 84696
189-267
gi|35504896
337-415
97%

Seq ID NO: 84696
473-526
gi|35504896
413-466
98%

Seq ID NO: 84696
40-98
gi|7143674
296-354
93%

Seq ID NO: 84696
42-98
gi|7144456
296-352
92%

Seq ID NO: 84696
40-92
gi|18382222
454-506
94%

Seq ID NO: 84696
40-89
gi|18090122
491-540
94%

Seq ID NO: 84696
55-92
gi|18381283
389-426
94%

Seq ID NO: 84779
145-167
gi|46985397
123-101
100%

Seq ID NO: 84814
20-58
gi|28916076
1-41
92%

Seq ID NO: 47631
473-497
gi|54549558
143-167
100%

Seq ID NO: 84950
15-54
gi|28916076
1-40
97%

Seq ID NO: 84950
8-35
gi|159473
34-61
100%

Seq ID NO: 84950
8-35
gi|2454547
227-200
100%

Seq ID NO: 84950
8-35
gi|551594
194-221
100%

Seq ID NO: 84950
8-35
gi|551595
669-696
100%

Seq ID NO: 84950
8-35
gi|18032254
34-61
100%

Seq ID NO: 84950
12-35
gi|18477256
279-256
100%

Seq ID NO: 84950
12-35
gi|18477260
325-302
100%

Seq ID NO: 84950
12-35
gi|18477262
634-611
100%

Seq ID NO: 84950
8-29
gi|18477259
22-1
100%

Seq ID NO: 84950
8-29
gi|18477261
22-1
100%

Seq ID NO: 84950
8-29
gi|37780968
22-1
100%

Seq ID NO: 84996
246-365
gi|32324211
375-494
84%

Table 2 Legend:

¹
H. glycines Clone ID No as set forth in Sequence Listing feature fields; searching the H. glycines sequence identifier in column 1 identifies the corresponding SEQ ID NO for that sequence

²nucleotide position in SEQ ID NO corresponding to Clone ID No in column 1 that matches with position of sequence of GeneID in adjacent cell on same row of table 2

³GeneID number of corresponding matching sequence hit from public database that matches with position of Clone ID No from column 1; derivative organism information is associated with the GeneID No.

⁴nucleotide position in GeneID that matches with nucleotides specified on same row corresponding to sequence of Clone ID SEQ ID NO

⁵percent identity between the two sequences in Clone ID and GeneID

Surprisingly the inventors have also discovered that some polynucleotides of the present invention exhibit homology with various insect pests of plants and animals, as illustrated in Table 3. This provides an opportunity to express in plant cells polynucleotides exemplified in Table 3 as double stranded RNA sequences, providing control of many of these insect pests of plants and animals. Mosquitoes, for example, are well known as vectors for spreading malaria, yellow fever, encephalitis, filarial parasites and other serious diseases. Male mosquitoes feed exclusively on plant nectar and on plant cell exudates, and female mosquitoes feed on plants when a blood meal is not available. The present invention therefore provides a means for applying the exemplary sequences as dsRNA molecules expressed in plant cells as a means for controlling nematode and insect pests by expression of sequences identified as representative of common sequences between the two species.

TABLE 3

SCN vcDNA Sequences and Insect Nucleotide Sequence Homologous

SEQ ID NO¹
Position²
Gene ID³
Position⁴
% identity⁵
Genus species⁶

Seq ID NO: 68821
138-159
CRA|agCT42044
945-966
100%

Anopheles gambiae

Seq ID NO: 79019
537-557
CRA|agCT43147
1437-1457
100%

Anopheles gambiae

Seq ID NO: 47443
622-642
CRA|agCT43876
833-853
100%

Anopheles gambiae

Seq ID NO: 73243
46-69
CRA|agCT44110
215-238
100%

Anopheles gambiae

Seq ID NO: 54820
116-136
CRA|agCT44330
197-177
100%

Anopheles gambiae

Seq ID NO: 65924
13-33
CRA|agCT44378
3440-3460
100%

Anopheles gambiae

Seq ID NO: 53889
589-609
CRA|agCT44871
13437-13457
100%

Anopheles gambiae

Seq ID NO: 66729
38-59
CRA|agCT45079
733-754
100%

Anopheles gambiae

Seq ID NO: 60455
259-279
CRA|agCT45391
1624-1644
100%

Anopheles gambiae

Seq ID NO: 69523
121-145
CRA|agCT45432
192-216
96%

Anopheles gambiae

Seq ID NO: 80670
47-67
CRA|agCT46846
1180-1160
100%

Anopheles gambiae

Seq ID NO: 81791
47-69
CRA|agCT46968
1752-1730
100%

Anopheles gambiae

Seq ID NO: 67053
313-339
CRA|agCT47874
1073-1098
96%

Anopheles gambiae

Seq ID NO: 59568
697-718
CRA|agCT48203
192-171
100%

Anopheles gambiae

Seq ID NO: 72334
238-258
CRA|agCT49436
729-749
100%

Anopheles gambiae

Seq ID NO: 63951
103-123
CRA|agCT49483
652-672
100%

Anopheles gambiae

Seq ID NO: 62555
130-150
CRA|agCT51096
2549-2569
100%

Anopheles gambiae

Seq ID NO: 46459
342-364
CRA|agCT51427
215-237
100%

Anopheles gambiae

Seq ID NO: 66786
307-327
CRA|agCT52597
1900-1920
100%

Anopheles gambiae

Seq ID NO: 68784
4-24
CRA|agCT55082
20600-20620
100%

Anopheles gambiae

Seq ID NO: 53634
761-804
CRA|agCT55207
620-577
89%

Anopheles gambiae

Seq ID NO: 53634
754-774
CRA|agCT55207
597-577
100%

Anopheles gambiae

Seq ID NO: 53635
58-101
CRA|agCT55207
577-620
89%

Anopheles gambiae

Seq ID NO: 53635
88-108
CRA|agCT55207
577-597
100%

Anopheles gambiae

Seq ID NO: 73360
270-291
CRA|agCT55621
744-723
100%

Anopheles gambiae

Seq ID NO: 82401
107-127
CRA|agCT55677
848-868
100%

Anopheles gambiae

Seq ID NO: 55012
650-670
EBI|221
281-261
100%

Anopheles gambiae

Seq ID NO: 78551
90-110
EBI|2300
624-644
100%

Anopheles gambiae

Seq ID NO: 68299
84-105
EBI|2307
2080-2101
100%

Anopheles gambiae

Seq ID NO: 47206
84-105
EBI|2307
2080-2101
100%

Anopheles gambiae

Seq ID NO: 68556
241-262
EBI|4053
3009-3030
100%

Anopheles gambiae

Seq ID NO: 70190
174-194
EBI|4283
5460-5440
100%

Anopheles gambiae

Seq ID NO: 53886
405-425
EBI|5326
2105-2085
100%

Anopheles gambiae

Seq ID NO: 70190
243-266
EBI|8982
1366-1389
100%

Anopheles gambiae

Seq ID NO: 51267
164-184
EBI|9090
121-141
100%

Anopheles gambiae

Seq ID NO: 55175
12-41
gi|11119314
36-7
100%

Andrya cuniculi

Seq ID NO: 66692
12-41
gi|11119314
36-7
100%

Andrya cuniculi

Seq ID NO: 55175
12-41
gi|11119315
36-7
100%

Paranoplocephala sp.

Seq ID NO: 66692
12-41
gi|11119315
36-7
100%

Paranoplocephala sp.

Seq ID NO: 55175
12-41
gi|11119317
36-7
100%

Paranoplocephala arctica

Seq ID NO: 66692
12-41
gi|11119317
36-7
100%

Paranoplocephala arctica

Seq ID NO: 55175
12-41
gi|11119319
36-7
100%

Paranoplocephala serrata

Seq ID NO: 66692
12-41
gi|11119319
36-7
100%

Paranoplocephala serrata

Seq ID NO: 47304
674-696
gi|23186984
477-499
100%

Echinococcus granulosus

Seq ID NO: 77038
16-43
gi|2463301
112-85
96%

Neogryporhynchus

cheilancristrotus

Seq ID NO: 59901
75-96
gi|31365321
176-155
100%

Toxoptera citricida

Seq ID NO: 51042
482-505
gi|31365444
320-297
100%

Toxoptera citricida

Seq ID NO: 77354
97-117
gi|31365580
644-664
100%

Toxoptera citricida

Seq ID NO: 82622
494-514
gi|37804570
57-77
100%

Rhopalosiphum padi

Seq ID NO: 66861
73-95
gi|46996593
400-378
100%

Acyrthosiphon pisum

Seq ID NO: 64007
76-96
gi|46996721
468-448
100%

Acyrthosiphon pisum

Seq ID NO: 60081
70-96
gi|46998065
535-562
96%

Acyrthosiphon pisum

Seq ID NO: 54610
316-337
gi|46998427
300-279
100%

Acyrthosiphon pisum

Seq ID NO: 47304
674-695
gi|47163116
387-408
100%

Echinococcus granulosus

Seq ID NO: 62977
216-237
gi|47514887
260-281
100%

Acyrthosiphon pisum

Seq ID NO: 58768
293-314
gi|47517134
76-55
100%

Acyrthosiphon pisum

Seq ID NO: 63826
72-92
gi|47522032
581-561
100%

Acyrthosiphon pisum

Seq ID NO: 82094
183-203
gi|47533062
209-189
100%

Acyrthosiphon pisum

Seq ID NO: 72433
558-578
gi|47536611
364-384
100%

Acyrthosiphon pisum

Seq ID NO: 57774
203-223
gi|47536768
381-401
100%

Acyrthosiphon pisum

Seq ID NO: 55175
12-41
gi|54306309
36-7
100%

Anoplocephaloides cf.

Seq ID NO: 66692
12-41
gi|54306309
36-7
100%

Anoplocephaloides cf.

Seq ID NO: 55175
12-41
gi|54306311
36-7
100%

Anoplocephaloides

kontrimavichusi

Seq ID NO: 66692
12-41
gi|54306311
36-7
100%

Anoplocephaloides

kontrimavichusi

Seq ID NO: 55175
12-41
gi|54306312
36-7
100%

Anoplocephaloides lemmi

Seq ID NO: 66692
12-41
gi|54306312
36-7
100%

Anoplocephaloides lemmi

Seq ID NO: 55175
12-41
gi|54306316
36-7
100%

Andrya rhopalocephala

Seq ID NO: 66692
12-41
gi|54306316
36-7
100%

Andrya rhopalocephala

Seq ID NO: 55175
12-41
gi|54306318
36-7
100%

Diandrya composita

Seq ID NO: 66692
12-41
gi|54306318
36-7
100%

Diandrya composita

Seq ID NO: 55175
12-41
gi|54306319
36-7
100%

Mosgovoyla pectinata

Seq ID NO: 66692
12-41
gi|54306319
36-7
100%

Mosgovoyla pectinata

Seq ID NO: 55175
12-41
gi|54306320
36-7
100%

Moniezia sp.

Seq ID NO: 66692
12-41
gi|54306320
36-7
100%

Moniezia sp.

Seq ID NO: 55175
12-41
gi|54306321
36-7
100%

Monoecocestus americanus

Seq ID NO: 66692
12-41
gi|54306321
36-7
100%

Monoecocestus americanus

Seq ID NO: 55175
12-41
gi|54306322
36-7
100%

Paranoplocephala

blanchardi

Seq ID NO: 66692
12-41
gi|54306322
36-7
100%

Paranoplocephala

blanchardi

Seq ID NO: 55175
12-41
gi|54306323
36-7
100%

Paranoplocephala etholeni

Seq ID NO: 66692
12-41
gi|54306323
36-7
100%

Paranoplocephala etholeni

Seq ID NO: 55175
12-41
gi|54306324
36-7
100%

Paranoplocephala fellmani

Seq ID NO: 66692
12-41
gi|54306324
36-7
100%

Paranoplocephala fellmani

Seq ID NO: 55175
12-41
gi|54306325
36-7
100%

Paranoplocephala gracilis

Seq ID NO: 66692
12-41
gi|54306325
36-7
100%

Paranoplocephala gracilis

Seq ID NO: 55175
12-41
gi|54306326
36-7
100%

Paranoplocephala

longivaginata

Seq ID NO: 66692
12-41
gi|54306326
36-7
100%

Paranoplocephala

longivaginata

Seq ID NO: 55175
12-41
gi|54306327
36-7
100%

Paranoplocephala

macrocephala

Seq ID NO: 66692
12-41
gi|54306327
36-7
100%

Paranoplocephala

macrocephala

Seq ID NO: 55175
12-41
gi|54306328
36-7
100%

Paranoplocephala cf.

Seq ID NO: 66692
12-41
gi|54306328
36-7
100%

Paranoplocephala cf.

Seq ID NO: 55175
12-41
gi|54306329
36-7
100%

Paranoplocephala kalelai

Seq ID NO: 66692
12-41
gi|54306329
36-7
100%

Paranoplocephala kalelai

Seq ID NO: 55175
12-41
gi|54306331
36-7
100%

Paranoplocephala

primordialis

Seq ID NO: 66692
12-41
gi|54306331
36-7
100%

Paranoplocephala

primordialis

Seq ID NO: 55175
12-41
gi|54306332
36-7
100%

Schizorchis sp.

Seq ID NO: 66692
12-41
gi|54306332
36-7
100%

Schizorchis sp.

Seq ID NO: 60083
406-426
gi|55794409
449-429
100%

Acyrthosiphon pisum

Seq ID NO: 62688
3-23
gi|55802365
769-789
100%

Acyrthosiphon pisum

Seq ID NO: 82614
269-290
gi|55803329
472-493
100%

Acyrthosiphon pisum

Seq ID NO: 54865
19-41
gi|55806106
709-731
100%

Acyrthosiphon pisum

Seq ID NO: 65500
126-146
gi|55810448
308-328
100%

Acyrthosiphon pisum

Seq ID NO: 80198
225-245
gi|55810583
245-265
100%

Acyrthosiphon pisum

Seq ID NO: 51184
74-94
gi|55814836
337-317
100%

Acyrthosiphon pisum

Seq ID NO: 52762
1-36
gi|6467344
700-665
91%

Duplicibothrium paulum

Table 3 Legend:

¹H. glycines Clone ID No as set forth in Sequence Listing feature fields; searching the H. glycines sequence identifier in column 1 identifies the corresponding SEQ ID NO for that sequence

²nucleotide position in SEQ ID NO corresponding to Clone ID No in column 1 that matches with position of sequence of Gene ID in column 3 on same row

³Gene ID number of corresponding matching sequence hit from public database that matches with position of Clone ID No from column 1; information in table is sorted by column 3

⁴Gene ID nucleotide position in column 3 that matches with nucleotides specified on same row corresponding to sequence of SCN Clone ID

⁵percent identity between the two sequences in Clone ID and Gene ID (comparison of identity between column 2 and column 4 sequences)

⁶Genus and species of organism corresponding to gene sequence set forth in Column 3

Example 6

This example illustrates the suppression of one or more genes in a soybean cyst nematode by providing in the diet of the nematode a double stranded RNA consisting of a nucleotide sequence that is complementary to the messenger RNA sequence expressed from the one or more soybean cyst nematode genes.

Soybean cyst nematode J2 larvae are treated with a double stranded RNA derived from a nucleotide sequence selected from the group consisting of SEQ ID NO:1-SEQ ID NO:97729 in a soaking assay as described in WO 03052110. Briefly, freshly hatched nematode larvae are treated in a soaking buffer (10 mM octopamine in M9 salts, 1 mg/ml FITC in DMF) with or without 2 microgram/microliter of dsRNA for four hours at room temperature. Larvae ingesting the solution are fluorescent. The fluorescent larvae are separated from non-fluorescent larvae, then inoculated into soil containing a germinated soybean seedling. The number of cysts on each plant are counted about 35 days after inoculation. The tested dsRNA molecules that demonstrate significant reduction in the number of cysts counted are then made into plant expression cassettes contained in DNA constructs designed for plant cell transformation. DNA constructs generally comprise constitutive promoters that cause transcription of a linked DNA that transcribes a dsRNA. Promoters that may exhibit enhanced expression in root tissue may be particularly useful for expressing dsRNA effective against soybean cyst nematodes.

These DNA constructs are transformed into soybean plant cells and the cells regenerated into plants. The plants are tested either as Ro plants for nematode resistance or seed is collected and the R1 seed is germinated and the R1 plant roots tested for nematode resistance. Resistance is demonstrated if the transgenic plants have a significant reduction in cyst number or cyst development.

Example 7

This example describes DNA constructs and the expression of a chimeric RNA molecule of the present invention in a transgenic soybean plant cell. The DNA constructs described herein comprise a promoter that causes transcription of an operably linked DNA into an RNA in a soybean cell, the DNA and the transcribed RNA of one or more segments exhibiting homology or complementarity to a soybean cyst nematode contiguous at least about 21-mer nucleotide sequence (DNA or RNA). Exemplary soybean cyst nematode DNA segments were previously described in Table 1 and are further identified in the Sequence Listing as SEQ ID NO:1-SEQ ID NO:45568. When expressed in a plant cell, the DNA construct provides an RNA transcript molecule comprising a self-complimentary segment, a portion of which folds into a double stranded RNA (dsRNA). Detection of the RNA transcript expressed in a cell or tissue of a transgenic plant is diagnostic for the DNA construct(s) that comprises a region of a soybean cyst nematode DNA molecule, and demonstrates that the DNA segment from which the dsRNA molecule is derived is transcribed/expressed in the transgenic soybean cells. Therefore, the transcribed RNA becomes available in the diet of the nematode as it feeds on a plant root cell. The RNA comprises a region that is double stranded and is complementary to a naturally occurring polynucleic acid molecule contained in a soybean cyst nematode cell, and when ingested by the nematode results in suppression of the normal level of the naturally occurring molecule.

Exemplary DNA constructs of the present invention have various regulatory elements that provide transcription or enhance expression or stability of an RNA molecule transcribed from a transgene in a plant cell. For example, a promoter element of a DNA construct of the present invention provides expression of an RNA transcript in a plant cell. Promoters, which can cause the transcription of a linked heterologous DNA are generally known in the art, for example, DNA plant virus promoters (P-CaMV35S, U.S. Pat. Nos. 5,352,605 and 5,196,525, comprising a duplicated enhancer region herein referred to as P-e35S; P-FMV35S, U.S. Pat. Nos. 5,378,619 and 5,018,100, herein incorporated by reference in their entirety), and various plant derived promoters, for example, plant actin promoters (P-Os.Act, U.S. Pat. Nos. 5,641,876 and 6,429,357, herein incorporated by reference in their entirety), and chimeric promoters, for example, P-FMV-Elf1α (U.S. Pat. No. 6,660,911 and other chimeric promoters disclosed therein, herein incorporated by reference in their entirety). Additionally, promoters that provide enhanced expression in root cells relative to other plant cells, may be tested and selected to express the RNA molecules of the present invention. The DNA constructs described in this example utilize the P-e35S and P-FMV promoters to drive the transcription of a DNA and expression of a dsRNA that exhibits homology to a portion of a soybean cyst nematode nucleotide sequence. For example, a nucleotide sequence was assembled consisting of two segments, the forward and reverse nucleotide sequence of SEQ ID NO:22219 from nucleotide position 552-699, linked by an amorphous 20-200 nucleotide segment that did not exhibit any known complementarity to the SCN genome sequences. Bioinformatics analysis indicates that the nucleotide sequence of SEQ ID NO:22219 corresponds to an open reading frame encoding an SCN specific proteasome A-type subunit peptide referred to herein as Pas-4. This chimeric sequence was incorporated into plant expression vectors for use in testing dsRNA mediated suppression of the pas-4 target gene. The DNA constructs 5749 (P-FMV/Pas-4-dsRNA/E6 3′ UTR) was thus assembled and comprises the necessary transfer molecules and regulatory molecules to provide integration into the genome of plant cells and expression of the dsRNA molecule therein.

The DNA constructs comprise a T-DNA region that is transferred into the genome of a plant cell by an Agrobacterium- or Rhizobium-mediated plant cell transformation method, and additional regulatory elements, for example, a 3′ untranslated region (3′ UTR) of the SIE6-3B gene from Gossypium barbadense, herein referred to as E6 3′ UTR (John Plant Mol Biol 30:297-306, 1996, NCBI accession U30508, nucleotide position from about 992-1304). The DNA construct 5749 (P-FMV/Pas-4-dsRNA/E6 3′ UTR) was transferred into Agrobacterium rhizogenes strain.

A transgenic root culture of soybean has been shown to support soybean cyst nematode infection and is useful for the expression of transgenes (Narayanan, et al., Crop Sci. 39:1680-1686, 1999 and Cho et al., Planta (2000) 210:195-204). Agrobacterium rhizogenes transformed to contain the described DNA construct 5749 was used to transformed soybean cells and create independent transgenic root cultures, referred to herein as events 5749-1, etc. Tissues from the transgenic root cultures were assayed for expression of the chimeric SCN gene suppression RNA molecule. Transgenic root tissues were selected using appropriate selection pressures. Transgenic root tissues from each event were screened for the presence of the fluorescence marker expression that was integrated into and adjacent to the dsRNA expression construct. The transgenic root tissues were also screened for the presence of siRNA segments produced from exposure to the root tissue cells' endogenous DICER molecules. siRNA segments were screened for identity to segments of the corresponding dsRNA coding sequences expressed from the plasmid construct expression cassettes. Methods for detecting the presence of an expressed RNA in a cell are known in the art. For example, in this example, the presence of the 3′ UTR was detected using primers that functioned to amplify the UTR sequence from the expressed RNA sequence. A TAQMAN method was then used along with a 3′ UTR specific fluorescence probe to detect the UTR as well as provide information on the relative level of expression from the construct. The data is shown in Table 4.

TABLE 4

Levels of Pas-4-dsRNA in Transgenic Soybean Root Cells.

Event
Ave fluorescense
St Dev
siRNA Northern

Vector control
0.00
0.00
ND

5749-1
2.30
1.05
+

5749-3
3.83
1.39
+

5749-4
5.47
2.44
+

5749-5
3.73
0.64
+

5749-8
0.45
0.14
ND

5749-10
0.16
0.02
ND

5749-11
0.24
0.04
+

5749-12
0.33
0.17
ND

5749-A
3.14
0.70
+

5749-B
2.91
0.27
+

ND — not detected

The data in Table 4 indicates that ten events comprising the Pas-4-dsRNA contained detectable levels of the RNA molecule. Northern blot analysis of these events showed detectable levels of siRNA that specifically hybridizes to DNA probes made from a homologous fragment of the Pas-4 coding region. These results demonstrate that soybean cells can be transformed with DNA constructs for expression of dsRNA molecules specific for gene suppression of SCN target genes, and that the transformed plant cells recognize the RNA molecules and dice them into detectable siRNA molecules that may be useful for specific gene suppression of the target gene(s) when provided in the diet of soybean cyst nematodes.

All patent publications cited in this specification are incorporated herein by reference to the same extent as if each individual publication or patent application was specifically and individually indicated to be incorporated by reference.

The Sequence Listing is submitted along with this specification on two compact discs. One disc is labeled ‘Sequence Listing’ according to 37 CFR §1.52(e)(4), and the other disc is labeled ‘CRF’ (computer readable form) according to 37 CFR §1.821(e). Each disc contains a single 271,645 kilo-byte text file labeled ‘SCN_seqListing.txt’, created on Feb. 22, 2005, in IBM-PC format and is compatible with IBM-PC, MS-Windows, Macintosh, and UNIX operating systems. The sequence listing information recorded in computer readable form is identical to the written compact disc sequence listing. The Sequence Listing text file is incorporated herein by reference.

	Number	Date	Country
Parent	11360355	Feb 2006	US
Child	12101830		US

METHODS FOR GENETIC CONTROL OF PLANT PEST INFESTATION AND COMPOSITIONS THEREOF

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