Claims
- 1. A method of identifying and isolating variant T4 DNA polymerases comprising the steps of:
- identifying T4 strains having variant T4 DNA polymerases defective in some aspect of DNA replication;
- isolating further mutated forms of said variant T4 DNA polymerases by selection in an E. coli optA1 host;
- isolating T4 strains which contain variant T4 DNA polymerases having at least one additional mutation which corrects or compensates said defect in DNA replication;
- identifying additional correcting/compensating mutation(s) in said variant T4 DNA polymerases; and
- introducing said identified correcting/compensating mutation(s) T4 DNA polymerases into T4 phage or T4 DNA polymerase expression vectors.
Parent Case Info
This application is a divisional of Ser. No. 08/101,593 filed Aug. 2, 1993 now U.S. Pat. No. 5,547,859.
Non-Patent Literature Citations (1)
Entry |
Reha-Krantz et al., Proc. Natl. Acad. Sci., 88:2417-2421 Mar. 1991. |
Divisions (1)
|
Number |
Date |
Country |
Parent |
101593 |
Aug 1993 |
|