Claims
- 1. A method of producing a composition comprising a substance which inhibits the specific binding between (i) a herpes simplex virus (HSV) ICP34.5 polypeptide and (ii) proliferating cell nuclear antigen (PCNA), said substance being identified by a method comprising(a) providing a first component selected from the group consisting of an HSV ICP34.5 polypeptide, the 63 amino acid C-terminus of ICP34.5, MyD116, and GADD34; (b) providing PCNA as a second component; (c) contacting the two components with a test substance under conditions that permit the two components to bind in the absence of said test substance; (d) determining whether said test substance inhibits binding of the first and second components; and (e) mixing a test substance determined in step (d) to inhibit said binding of the first and second components with a carrier or diluent to produce said composition.
- 2. The method according to claim 1 wherein said substance induces growth arrest and/or cell death.
- 3. The method according to claim 2 wherein said mammalian cell is a tumour cell.
- 4. The method according to claim 1 wherein said substance prevents cell death.
- 5. The method according to claim 4 wherein said mammalian cell is a cell of the central or peripheral nervous system.
Priority Claims (2)
Number |
Date |
Country |
Kind |
9705299 |
Mar 1997 |
GB |
|
9719919 |
Sep 1997 |
GB |
|
Parent Case Info
This is a continuation of application Ser. No. 09/380,760, filed Nov. 29, 1999, now U.S. Pat. No. 6,235,467 which is a 371 of PCT/GB98/00772, filed Mar. 16, 1998, the entire content of which is hereby incorporated by reference in this application.
US Referenced Citations (1)
Number |
Name |
Date |
Kind |
5834216 |
Roizman et al. |
Nov 1998 |
A |
Foreign Referenced Citations (4)
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Date |
Country |
WO 96 14334 |
May 1996 |
WO |
WO 96 35715 |
Nov 1996 |
WO |
WO 97 10349 |
Mar 1997 |
WO |
WO 96 04726 |
Feb 1998 |
WO |
Non-Patent Literature Citations (4)
Entry |
Bowie et al. (Science, 1990, 257: 1306-1310).* |
Burgess et al. (J. of Cell Bio. 111:2129-2138, 1990).* |
Lazar et al. (Molecular and Cellular Biology, 1988, 8:1247-1252).* |
Bork (Genome Research, 2000, 10:398-400). |
Continuations (1)
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Number |
Date |
Country |
Parent |
09/380760 |
|
US |
Child |
09/750099 |
|
US |