Claims
- 1. A method for increasing stearate as a component of total triglycerides found in soybean seed, wherein said method comprises:
growing a soybean plant having integrated into its genome a DNA construct, said construct comprising in the 5′ to 3′ direction of transcription, a promoter functional in a soybean plant seed cell, a DNA sequence encoding an acyl-ACP thioesterase protein having substantial activity on C18:0 acyl-ACP substrates, and a transcription termination region functional in a plant cell.
- 2. The method of claim 1, wherein said encoding sequence has little or no activity on C16:0 substrates.
- 3. The method of claim 1 wherein said thioesterase encoding sequence is from the genus Garcinia.
- 4. The method of claim 1, wherein said DNA sequence encodes the Garm FatA1 thioesterase presented in FIG. 1.
- 5. The method of claim 1, wherein said promoter is from a gene preferentially expressed in plant seed tissue.
- 6. The method according to claim 1, wherein said promoter is the napin promoter.
- 7. The method according to claim 1, wherein said promoter is from the soybean β-conglycinin 7S subunit transcription initiation region.
- 8. The method of claim 1, wherein said plant seed triglycerides comprise about 20 weight percent or greater stearate.
- 9. The method of claim 1, wherein said plant seed triglycerides comprise about 33 weight percent or greater stearate.
- 10. The method of claim 1, wherein said plant seed triglycerides comprise about 40 weight percent or greater stearate.
- 11. The method of claim 1, wherein said plant seed triglycerides comprise about 50 weight percent or greater stearate.
- 12. A soybean seed comprising about 33 weight percent or greater stearate as a component of total fatty acids found in seed triglycerides.
- 13. The soybean seed according to claim 12 comprising about 40 weight percent or greater stearate as a component of said seed triglycerides.
- 14. The soybean seed according to claim 13 comprising about 50 weight percent or greater stearate as a component of said seed triglycerides.
- 15. The soybean seed according to claim 12 comprising about 50 percent or greater stearate as the fatty acid found at the sn-1 and sn-3 positions of seed triglycerides.
- 16. The soybean seed according to claim 13 comprising about 60 percent or greater stearate as the fatty acid found in said sn-1 and sn-3 positions of seed triglycerides.
- 17. The soybean seed according to claim 14 comprising about 75 percent or greater stearate as the fatty acid found in said sn-1 and sn-3 positions of seed triglycerides.
- 18. Soybean seed according to claim 15, and comprising oleic acid as the predominant fatty acid at the sn-2 position of seed triglycerides.
- 19. Seed oil obtained from seed of claim 12.
- 20. A shortening comprising the seed oil of claim 19.
- 21. A soybean plant having seed comprising about 50 weight percent or greater saturated fatty as a component total fatty acids found in seed triglycerides.
- 22. The soybean plant according to claim 21 wherein said saturated fatty acid component is about 60 weight percent or greater.
- 23. The soybean plant according to claim 22 wherein said saturated fatty acid component is about 65 weight percent or greater.
- 24. A soybean plant having seed comprising about 20 weight percent or greater stearate and about 15 weight percent or less oleate as components of total fatty acids found in seed triglycerides.
- 25. The soybean plant according to claim 24 wherein said stearate content is greater than about 33% of the total fatty acid composition.
- 26. A vegetable oil-based edible spread comprising a high stearate soybean oil.
- 27. The vegetable oil-based edible spread of claim 26, wherein said soybean oil comprises a stearate level of about 20 weight percent or greater.
- 28. The vegetable oil-based edible spread of claim 26, wherein said soybean oil is the seed oil of the mutant soybean variety HS2.
- 29. The vegetable oil-based edible spread of claim 26, wherein said soybean oil is the seed oil of claim 19.
- 30. A frying oil comprising a high stearate soybean oil.
- 31. The frying oil of claim 30, wherein said soybean oil comprises a stearate level of about 20 weight percent or greater.
- 32. The frying oil of claim 30, wherein said soybean oil is the seed oil of the mutant soybean variety HS2.
- 33. The frying oil of claim 30, wherein said soybean oil is the seed oil of claim 19.
- 34. A cocoa butter substitute comprising a high stearate soybean oil.
- 35. The cocoa butter substitute of claim 34, wherein said soybean oil comprises a stearate level of about 20 weight percent or greater.
- 36. The cocoa butter substitute of claim 34, wherein said soybean oil is the seed oil of the mutant soybean variety HS2.
- 37. The cocoa butter substitute of claim 34, wherein said soybean oil is the seed oil of claim 19.
- 38. A cocoa butter equivalent comprising a high stearate soybean oil.
- 39. The cocoa butter equivalent of claim 38, wherein said soybean oil comprises a stearate level of about 20 weight percent or greater.
- 40. The cocoa butter equivalent of claim 38, wherein said soybean oil is the seed oil of the mutant soybean variety HS2.
- 41. The cocoa butter equivalent of claim 38, wherein said soybean oil is the seed oil of claim 19.
- 42. A soymilk produced from soybean seed having a high stearate oil.
- 43. The soymilk of claim 42, wherein said oil comprises a stearate level of about 20 weight percent or greater.
- 44. The soymilk of claim 42, wherein said oil is the seed oil of the mutant soybean variety HS2.
- 45. The soymilk of claim 42, wherein said oil is the seed oil of claim 19.
- 46. A tofu product produced from soybean seed having a high stearate oil.
- 47. The tofu product of claim 46, wherein said oil comprises a stearate level of about 20 weight percent or greater.
- 48. The tofu product of claim 46, wherein said oil is the seed oil of the mutant soybean variety HS2.
- 49. The tofu product of claim 46, wherein said oil is the seed oil of claim 19.
- 50. A flour produced from soybean seed having a high stearate oil.
- 51. The flour of claim 50, wherein said oil comprises a stearate level of about 20 weight percent or greater.
- 52. The flour of claim 50, wherein said oil is the seed oil of the mutant soybean variety HS2.
- 53. The flour of claim 50, wherein said oil is the seed oil of claim 19.
Parent Case Info
[0001] This application is a continuation-in-part of Application U.S. Ser. No. 09/134,26 filed Aug. 14, 1998.
Continuations (1)
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Number |
Date |
Country |
Parent |
09359070 |
Jul 1999 |
US |
Child |
10061280 |
Feb 2002 |
US |
Continuation in Parts (1)
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Number |
Date |
Country |
Parent |
09134262 |
Aug 1998 |
US |
Child |
09359070 |
Jul 1999 |
US |