Claims
- 1. A method for introducing a nucleic acid into a mammalian cell, said method comprising contacting said cell with a composition comprising a nucleic acid and a compound having the formula: whereinR1 and R2 each independently represent a C8-C24 saturated or unsaturated hydrocarbon chain, uninterrupted or interrupted by from 1 to 3 heteroatom moieties selected from the group consisting of —O—, —S—, —NH— and —NR—; X represents a member selected from the group consisting of —CH2—, —O—, —S—, —NH— and —NR—; wherein R is a lower alkyl group having from 1 to 4 carbon atoms; n is an integer of from 1 to 2; and A− is an anion.
- 2. A method in accordance with claim 1, wherein R1 and R2 each independently represent a C8-C24 saturated or unsaturated hydrocarbon chain.
- 3. A method in accordance with claim 1, wherein R1 and R2 each independently represent a C12-C20 saturated hydrocarbon chain.
- 4. A method in accordance with claim 1, wherein R1 and R2 each independently represent a C12-C20 unsaturated hydrocarbon chain.
- 5. A method in accordance with claim 1, wherein n is 1.
- 6. A method in accordance with claim 1, wherein R1 and R2 each independently represent a C12-C16 unsaturated hydrocarbon chain, X is —O—, and n is 1.
- 7. A method in accordance with claim 1, wherein R1 represents a C18-unsaturated hydrocarbon chain, R2 represents a C17 unsaturated hydrocarbon chain, X is —O—, and n is 1.
- 8. A method in accordance with claim 1, wherein R1 and R2 each contain a single double bond in the cis orientation.
- 9. A method in accordance with claim 1, wherein said compound has the formula:
- 10. A method in accordance with claim 1, wherein said compound has the formula:
- 11. A method in accordance with claim 1, wherein said composition further comprises cholesterol.
- 12. A method in accordance with claim 1, wherein said composition further comprises dilauroylphosphatidylethanolamine.
- 13. A method in accordance with claim 1, wherein said composition is in the form of unilamellar vesicles.
- 14. A method in accordance with claim 1, wherein said nucleic acid is part of an expression cassette.
- 15. A method in accordance with claim 1, wherein said nucleic acid is complexed with liposomes comprising said compound.
- 16. A method in accordance with claim 1, wherein said nucleic acid encodes a therapeutic protein.
- 17. A method in accordance with claim 1, wherein said nucleic acid encodes a ribozyme.
Parent Case Info
This application is a divisional of application Ser No. 09/183,634, filed Oct. 30, 1998, now U.S. Pat No. 6,121,457 which claims priority from Ser. No. 60/065,859 filed Nov. 14, 1997, the disclosures of each being incorporated by reference.
US Referenced Citations (7)
Foreign Referenced Citations (1)
Number |
Date |
Country |
95-14380 |
Jun 1995 |
WO |
Provisional Applications (1)
|
Number |
Date |
Country |
|
60/065859 |
Nov 1997 |
US |