Claims
- 1. A method for monitoring differential expression of a plurality of genes in a first filamentous fungal cell relative to expression of the same genes in one or more second filamentous fungal cells, comprising:
(a) adding a mixture of fluorescence-labeled nucleic acids isolated from the filamentous fungal cells to a substrate containing an array of Fusarium venenatum ESTs selected from the group consisting of SEQ ID NOs. 1-3770 and fragments thereof, under conditions where the nucleic acids hybridize to complementary sequences of the ESTs in the array, wherein the nucleic acids from the first filamentous fungal cell are labeled with a first fluorescent reporter and the one or more second filamentous fungal cells are labeled with one or more different second fluorescent reporters; and (b) examining the array by fluorescence under fluorescence excitation conditions wherein the relative expression of the genes in the filamentous fungal cells is determined by the observed fluorescence emission color of each spot in the array in which (i) the fluorescence-labeled nucleic acids obtained from the first filamentous fungal cell that are hybridized to the ESTs in the array produce a distinct first fluorescence emission color and the fluorescence-labeled nucleic acids obtained from the one or more second filamentous fungal cells that are hybridized to the ESTs in the array produce a distinct second fluorescence emission color, and (ii) the fluorescence-labeled nucleic acids obtained from both the first and the one or more second filamentous fungal cells that are hybridized to the ESTs in the array produce a distinct combined fluorescence emission color.
- 2. The method of claim 1, wherein one or more of the filamentous fungal cells are selected from the group consisting of an Acremonium, Aspergillus, Fusarium, Humicola, Mucor, Myceliophthora, Neurospora, Penicillium, Thielavia, Tolypocladium, and Trichoderma cell.
- 3. The method of claim 1, wherein the two or more filamentous fungal cells are the same cell.
- 4. The method of claim 1, wherein the two or more filamentous fungal cells are Fusarium venenatum cells.
- 5. The method of claim 103, wherein the two or more filamentous fungal cells are Aspergillus niger cells.
- 6. The method of claim 103, wherein the two or more filamentous fungal cells are Aspergillus oryzae cells.
- 7. The method of claim 103, wherein the two or more filamentous fungal cells are different cells.
- 8. The method of claim 103, wherein the hybridization conditions are selected from the group consisting of very low, low, low-medium, medium, medium-high, high, and very high stringency conditions.
CROSS-REFERENCE TO RELATED APPLICATION
[0001] This application is a divisional of pending U.S. application Ser. No. 09/533,559 filed Mar. 22, 2000, which is a continuation-in-part of U.S. application Ser. No. 09/273,623 filed Mar. 22, 1999, now abandoned, which applications are fully incorporated herein by reference.
Divisions (1)
|
Number |
Date |
Country |
Parent |
09533559 |
Mar 2000 |
US |
Child |
10653047 |
Aug 2003 |
US |
Continuation in Parts (1)
|
Number |
Date |
Country |
Parent |
09273623 |
Mar 1999 |
US |
Child |
09533559 |
Mar 2000 |
US |