METHODS FOR PRINTING CELLS AND GENERATING ARRAYS OF BARCODED CELLS

Abstract

This disclosure relates to compositions and methods for analyzing single cells using cell printing and spatial analysis.

Description

Claims

1. A method for determining a location of an analyte in a cell comprising: (a) separating the cell from a plurality of cells;(b) printing the cell onto a surface comprising an array, wherein the array comprises a plurality of capture probes, wherein a capture probe of the plurality of capture probes comprises: (i) a spatial barcode and (ii) a capture domain;(c) hybridizing the analyte to the capture domain; and(d) determining (i) all or a part of the sequence of the analyte bound to the capture domain, or a complement thereof, and (ii) all or a part of the sequence of the spatial barcode, or a complement thereof, and using the determined sequence of (i) and (ii) to identify the location of the analyte in the cell.